Rw2G011250

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000105
Physical Location & Seq
Forward (+)
11365617 .. 11371680
6064 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G011250.1

Sequence Viewer

Length: 3156 bp
ATGGAGCGAGATATCATACCAGTCTACTATGAAATGGATACATTGCTATTACGGCAACAAGGGGGAGTTAATAAGATCCTTTTGAGACCGGAAGTAACAGAACATGAAGAAGTTTATTGGGAGAAATGGGACAAACTAAATATGAGGCAACAACAGTGCAGGGATGCTTTGGTACAGCTGTGCTACATAGATGGGTTTGATCTGGACTCCGAGAATGTGCCAGAAACTATAATAGGTCAAATTTGTGGCGTAGTAAAGAAATCGTATACACCATCTACAAATGTACACAAGGGCATGGTAGGACTACAAAAGGGCATGGTAGGACTGGAATCCCGCATTCATGATTTAGTACGTAATTATGTATGTCCACATTTTCTTAACGTACGTATCATAGGAATCCATGGAATGCGAGGAATAGGTAAGACAACTGTTGCCCGAGCTGTTTATCATCATATTCGTGATGGTTTTGAAAGTAGCTGCTTTCTTTCCAATGTTAGAGAAAGGTCCAAAAAGGATGGTCTAGTCTCTTTACAAGAAGAACTTCTTTCCAGAATGCTGTCTACAGAAATTGGAGTAGAGGATGAGTATGCAGGAACTGCTCTGATAAAAAAATGGTTATGCAGGCGAAGAGTACTTGTTGTCATTGATGATGTGGATGATCTAACACAATTGGAAAATTTGGCTGGATGTCATGACTGGTTCGGCCTGGGGAGTAGAATTATCATAACTACCACAGATGTTCATTTATTGAAGGCATATGATGATGGTGTTGATGGTATATATGAGGCGACTGGATTACTATATGGTGAATCTCTTGAACTTTTATGTTTGAAGGCTTTTAAGAAGAGCCAACCGCCTGAAGATCATTTGCACCTGTGCCACCATATTTTGAATTATGCATGTGGGCTGCCATTAGCTCTTGAGGTCATTGGTTCCTTTCTATGTGGTAGAAGCATGGGAGAATGGGTAAGTGCCATCGGTAGGCTAAAGAATAAACCAGAAAGAAGTATTATCGATGTGCTTCAGATAAGTTTTGATGGACTAGGGGAAACAGAAAAGGAAATATTCCTACATATTGCATGCTTTTATAAGGGGAAATCCAGGGATCGTGTAATACAAATACTCGACTACTGTCAGCTTTCCCCTGTTATTGGTTTAAGTGTTCTTGTTGACAAATCTCTCATTACTATTTCCAACAACAAACTGTTGATGCATGATTTACTACAAGAAATGGGCTGGGAAATAGTTCGCCGAGAGTCTCCTAATAATCCAGGCAAACGTAGTAGATTATGGTCTCATGAGGACATCCACACTGTGCTGAAGAAAGATAAGGGAACAGAAGCAGTTCAAGGCATGGTGATGGACTTGCCCAAGTTAGAAGTGGCTCAGTGGAAACCGGAAGCCTTTTCAAATTTGTCTCAACTTAGTTTCCTCCAAATTCGAAATGTGGACCTTCCCAAAGGCCCCACTTGTCTCTCTAATTCGTTGAAACTCCTCGAATGGAGTAGATTCCCTTTAAGGTTTCTCCCACAAGATTTTGAATCAGATGAACTCATTGAACTTAACTTGTGCCACAGCAATATTGAACAGCTTTGGCAAGGAGCAAAGAACTTTGACAAGTTGAAGTTCCTCAAACTCTGCCATTCTGAAAGCCTTTGTGAGACCCCAGACTTCACAGGCGTTCAAAATCTTGAACGATTAGATCTTGAAGGATGTAAAAAGTTGGTTAGAATTCATCCATCCCTTGGAGTTCTCAGAAAGCTTATTCTATTGAATCTTAAAGACTGTGAAAGTCTCATGAGTCTGCCAGATAAAATCAAAATGGAATCTCTCGAAACTCTAATTCTTTCAAACTGCTCAAGTCTTAAGAAGATCCCTGAGTTTGTTGGATATATGGAACGTTTGTTGGTGCTTAATCTTGACAAGACTGCCATTGAAACACTTCCTCCCTCAATTGAGCGCTTAAGTGGCCTTGTTTCCTTGGATTTAAGCAACTGCAAAAATCTTGTTTCTCTTCCTCACACTATTAATGGATTGAAGTCCCTCCAAAATCTAAGTCTTTCTGGATGCTTGAAACTTGGCGAACATCAGGGTAGTGTAAGGGAGATGGAGTATATGGAGGGAAATGGTGCAAGTAGAACTGCTGTAGCAGAGTTGCCATCATGGAAATATAGTTTAAGCCATTGTTTTGAAGTGGTCCAGGAACATTTCCCATGGTCCTTCCCTTCTAGATCAATGCAAAGAGTCAATACAGAACCAATGAGTATGCAGTTGCATTTATCTGGTCTCTGTAATTTAACAGACCTAAACATAAGCAATTGCAATCTCGGTGATGGAGCATTTCCCAACCACTTTGGCTGCTTTCCCTCATTGGTGGCATTGAATCTGAGTGGAAATGATTTTGTTAAGCTTCCTTCAAGCATCAAATCGTTTCTTAAGCTTGAAAACCTTAATTTGGAGAATTGCAAGAGACTTAAAGAGTTGTCAGAGCTTCCATCAAATAGTAAATTGGATGTAAGGGCAGATGGCTGTTCATCACTGGACATGCTGTTTGATGCATCCAATTTCAATAGATTGGAGAAATCATATTTCACTTTCATCAATTGCTTCAGACTAAATGACAATCAAGGCTGCAGGAACATAGCATTTGAAATGCTGAAGACATTCATTCCTCAGGTTATCTCGAATGCAAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAAAATTCCTGAGTGGTTCGATCATCAAAGTTTTGGGAGTTCATTAAGTGTACAGTTGCCTCCATTTTGGAGTAACAGTAAGTTTATGGGGTTTGCTTTTTGTGCTGTTTTTGTACTCGATGAGCACCGTGGAGTTGATATACTTGGCCAGCATCAATTCAAGACTTTCAATGCTACACACCATCTTGTATGTTGCATGTCCCTAAATGGGTTCGAGTTAAAACAAATTTATGGCAAACAGCCTGCATTTCGCTTTAGTGAACAATTTTGCCAGGTTAAGTCAGATCACCTTTGGACATTCTATGTATCCCGCGATAAATACTTTGCTGCAGAATGGTGGGATAGCAGTTGCAGTCAGCTTGAGTTCTTATTTAAAACCAGAGGGCCAGGTCTGAAGGTGAAGAAGTGCGGGGTCCATTTGATATATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1051

Amino Acids

119.5

Weight (kDa)

6.34

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 127 - 283 1.6e-17 NB-ARC domain
NACHT PF05729 130 - 226 7e-06 NACHT domain
WHD_ROQ1 PF23282 350 - 420 2.4e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 609 - 696 7.4e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 632 - 865 5.4e-10 Leucine-rich repeat region
C-JID PF20160 905 - 1051 5.7e-20 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1089
AccB7I CCANNNNNTGG 1 cut(s) 1745
AccI GTMKAC 3 cut(s) 24, 266, 560
AccII CGCG 1 cut(s) 3036
AciI CCGC 4 cut(s) 334, 854, 3034, 3132
AclI AACGTT 1 cut(s) 1900
AclWI GGATC 3 cut(s) 70, 1113, 1867
AcoI YGGCCR 1 cut(s) 2869
AcsI RAATTY 7 cut(s) 240, 676, 1411, 1437, 1731, 2727, 2949
AcuI CTGAAG 6 cut(s) 879, 1007, 1340, 2593, 2678, 3137
AdeI CACNNNGTG 1 cut(s) 1315
AfaI GTAC 7 cut(s) 174, 285, 351, 384, 633, 2775, 2838
AfeI AGCGCT 1 cut(s) 1961
AfiI CCNNNNNNNGG 5 cut(s) 981, 1151, 1745, 2455, 2931
AflII CTTAAG 3 cut(s) 1865, 1963, 2435
AjnI CCWGG 7 cut(s) 705, 1100, 1270, 2199, 2716, 2994, 3109
Alw21I GWGCWC 1 cut(s) 2850
AlwI GGATC 3 cut(s) 70, 1113, 1867
AlwNI CAGNNNCTG 1 cut(s) 596
Ama87I CYCGRG 1 cut(s) 435
Aor51HI AGCGCT 1 cut(s) 1961
AoxI GGCC 5 cut(s) 703, 1462, 1969, 2869, 3107
ApeKI GCWGC 5 cut(s) 477, 907, 2358, 2631, 3050
ApoI RAATTY 7 cut(s) 240, 676, 1411, 1437, 1731, 2727, 2949
ArsI GACNNNNNNTTYG 4 cut(s) 2041, 2073, 2534, 2566
AseI ATTAAT 1 cut(s) 2028
Asp700I GAANNNNTTC 6 cut(s) 540, 1245, 1344, 1941, 2663, 2700
AspLEI GCGC 1 cut(s) 1962
AspS9I GGNCC 7 cut(s) 504, 1450, 1463, 2197, 2217, 3107, 3136
AsuHPI GGTGA 5 cut(s) 818, 1369, 2342, 3002, 3133
AsuII TTCGAA 1 cut(s) 1441
AvaI CYCGRG 1 cut(s) 435
AvaII GGWCC 5 cut(s) 504, 1450, 2197, 2217, 3136
AxyI CCTNAGG 1 cut(s) 2673
BaeI ACNNNNGTAYC 4 cut(s) 164, 197, 370, 403
BalI TGGCCA 1 cut(s) 2871
BbsI GAAGAC 1 cut(s) 2666
Bbv12I GWGCWC 1 cut(s) 2850
BbvI GCAGC 5 cut(s) 464, 894, 2345, 2618, 3037
BceAI ACGGC 1 cut(s) 68
BciT130I CCWGG 7 cut(s) 707, 1102, 1272, 2201, 2718, 2996, 3111
BciVI GTATCC 2 cut(s) 31, 3040
BfaI CTAG 3 cut(s) 521, 1043, 2229
BfmI CTRYAG 4 cut(s) 561, 2145, 2632, 3051
BfoI RGCGCY 1 cut(s) 1963
BfrI CTTAAG 3 cut(s) 1865, 1963, 2435
BfuI GTATCC 2 cut(s) 31, 3040
BglII AGATCT 1 cut(s) 1702
BisI GCNGC 5 cut(s) 478, 908, 2359, 2632, 3051
BlsI GCNGC 5 cut(s) 479, 909, 2360, 2633, 3052
BmcAI AGTACT 1 cut(s) 633
Bme1390I CCNGG 7 cut(s) 707, 1102, 1272, 2201, 2718, 2996, 3111
Bme18I GGWCC 5 cut(s) 504, 1450, 2197, 2217, 3136
BmeT110I CYCGRG 1 cut(s) 435
BmgT120I GGNCC 7 cut(s) 504, 1450, 1463, 2197, 2217, 3107, 3136
BmiI GGNNCC 3 cut(s) 934, 1465, 3137
BmrFI CCNGG 7 cut(s) 707, 1102, 1272, 2201, 2718, 2996, 3111
BmsI GCATC 7 cut(s) 154, 1200, 2057, 2430, 2545, 2567, 2884
BoxI GACNNNNGTC 1 cut(s) 1131
BpiI GAAGAC 1 cut(s) 2666
Bpu14I TTCGAA 1 cut(s) 1441
BpuEI CTTGAG 3 cut(s) 941, 1843, 3104
Bsa29I ATCGAT 1 cut(s) 1014
BsaAI YACGTR 2 cut(s) 353, 386
BsaI GGTCTC 4 cut(s) 79, 1299, 1655, 2291
BsaJI CCNNGG 7 cut(s) 400, 706, 1101, 1744, 1980, 2213, 2851
BsaWI WCCGGW 2 cut(s) 88, 1396
BsaXI ACNNNNNCTCC 8 cut(s) 951, 981, 1930, 1960, 2096, 2126, 2785, 2815
Bsc4I CCNNNNNNNGG 5 cut(s) 981, 1151, 1745, 2455, 2931
Bse1I ACTGG 5 cut(s) 20, 330, 701, 796, 2544
Bse21I CCTNAGG 1 cut(s) 2673
Bse3DI GCAATG 1 cut(s) 41
BseBI CCWGG 7 cut(s) 707, 1102, 1272, 2201, 2718, 2996, 3111
BseCI ATCGAT 1 cut(s) 1014
BseDI CCNNGG 7 cut(s) 400, 706, 1101, 1744, 1980, 2213, 2851
BseLI CCNNNNNNNGG 5 cut(s) 981, 1151, 1745, 2455, 2931
BseMI GCAATG 1 cut(s) 41
BseMII CTCAG 6 cut(s) 1400, 1768, 1869, 2378, 2687, 2724
BseNI ACTGG 5 cut(s) 20, 330, 701, 796, 2544
BseRI GAGGAG 1 cut(s) 1484
BseXI GCAGC 5 cut(s) 464, 894, 2345, 2618, 3037
BseYI CCCAGC 1 cut(s) 1236
BsgI GTGCAG 1 cut(s) 178
Bsh1236I CGCG 1 cut(s) 3036
BshFI GGCC 5 cut(s) 705, 1464, 1971, 2871, 3109
BshVI ATCGAT 1 cut(s) 1014
BsiHKAI GWGCWC 1 cut(s) 2850
BsiHKCI CYCGRG 1 cut(s) 435
BsiSI CCGG 2 cut(s) 89, 1397
BsiWI CGTACG 1 cut(s) 382
BslFI GGGAC 3 cut(s) 143, 2026, 2908
BslI CCNNNNNNNGG 5 cut(s) 981, 1151, 1745, 2455, 2931
BsmFI GGGAC 3 cut(s) 143, 2026, 2908
BsmI GAATGC 4 cut(s) 336, 411, 558, 2692
BsnI GGCC 5 cut(s) 705, 1464, 1971, 2871, 3109
Bso31I GGTCTC 4 cut(s) 79, 1299, 1655, 2291
BsoBI CYCGRG 1 cut(s) 435
Bsp119I TTCGAA 1 cut(s) 1441
Bsp1286I GDGCHC 1 cut(s) 2850
Bsp1407I TGTACA 2 cut(s) 283, 2773
Bsp19I CCATGG 2 cut(s) 400, 2213
BspACI CCGC 4 cut(s) 334, 854, 3034, 3132
BspANI GGCC 5 cut(s) 705, 1464, 1971, 2871, 3109
BspCNI CTCAG 6 cut(s) 1399, 1767, 1870, 2379, 2686, 2725
BspDI ATCGAT 1 cut(s) 1014
BspFNI CGCG 1 cut(s) 3036
BspHI TCATGA 4 cut(s) 340, 691, 1297, 1797
BspLI GGNNCC 3 cut(s) 934, 1465, 3137
BspMAI CTGCAG 2 cut(s) 2636, 3055
BspPI GGATC 3 cut(s) 70, 1113, 1867
BspQI GCTCTTC 1 cut(s) 839
BspT104I TTCGAA 1 cut(s) 1441
BspTI CTTAAG 3 cut(s) 1865, 1963, 2435
BspTNI GGTCTC 4 cut(s) 79, 1299, 1655, 2291
BsrDI GCAATG 1 cut(s) 41
BsrGI TGTACA 2 cut(s) 283, 2773
BsrI ACTGG 5 cut(s) 20, 330, 701, 796, 2544
BssECI CCNNGG 7 cut(s) 400, 706, 1101, 1744, 1980, 2213, 2851
BssNAI GTATAC 1 cut(s) 267
BssT1I CCWWGG 4 cut(s) 400, 1744, 1980, 2213
Bst1107I GTATAC 1 cut(s) 267
Bst2UI CCWGG 7 cut(s) 707, 1102, 1272, 2201, 2718, 2996, 3111
Bst4CI ACNGT 9 cut(s) 156, 430, 1133, 1206, 1315, 1787, 2778, 2801, 2852
Bst6I CTCTTC 3 cut(s) 622, 839, 2019
BstAFI CTTAAG 3 cut(s) 1865, 1963, 2435
BstAPI GCANNNNNTGC 1 cut(s) 596
BstAUI TGTACA 2 cut(s) 283, 2773
BstBAI YACGTR 2 cut(s) 353, 386
BstBI TTCGAA 1 cut(s) 1441
BstC8I GCNNGC 4 cut(s) 623, 1081, 2873, 2967
BstDEI CTNAG 8 cut(s) 1386, 1424, 1754, 1878, 2054, 2387, 2673, 2733
BstDSI CCRYGG 3 cut(s) 400, 2213, 2851
BstFNI CGCG 1 cut(s) 3036
BstH2I RGCGCY 1 cut(s) 1963
BstHHI GCGC 1 cut(s) 1962
BstMWI GCNNNNNNNGC 4 cut(s) 52, 596, 1968, 2825
BstNI CCWGG 7 cut(s) 707, 1102, 1272, 2201, 2718, 2996, 3111
BstNSI RCATGY 4 cut(s) 903, 1083, 2548, 2923
BstPAI GACNNNNGTC 1 cut(s) 1131
BstSCI CCNGG 7 cut(s) 705, 1100, 1270, 2199, 2716, 2994, 3109
BstSFI CTRYAG 4 cut(s) 561, 2145, 2632, 3051
BstSNI TACGTA 2 cut(s) 353, 386
BstUI CGCG 1 cut(s) 3036
BstV1I GCAGC 5 cut(s) 464, 894, 2345, 2618, 3037
BstV2I GAAGAC 1 cut(s) 2666
BstX2I RGATCY 3 cut(s) 75, 1702, 1872
BstYI RGATCY 3 cut(s) 75, 1702, 1872
BstZ17I GTATAC 1 cut(s) 267
Bsu15I ATCGAT 1 cut(s) 1014
Bsu36I CCTNAGG 1 cut(s) 2673
BsuI GTATCC 2 cut(s) 31, 3040
BsuRI GGCC 5 cut(s) 705, 1464, 1971, 2871, 3109
BsuTUI ATCGAT 1 cut(s) 1014
BtgI CCRYGG 3 cut(s) 400, 2213, 2851
BtsIMutI CAGTG 4 cut(s) 161, 1311, 1394, 2537
Cac8I GCNNGC 4 cut(s) 623, 1081, 2873, 2967
CaiI CAGNNNCTG 1 cut(s) 596
CciI TCATGA 4 cut(s) 340, 691, 1297, 1797
CfoI GCGC 1 cut(s) 1962
Cfr13I GGNCC 7 cut(s) 504, 1450, 1463, 2197, 2217, 3107, 3136
ClaI ATCGAT 1 cut(s) 1014
Csp6I GTAC 7 cut(s) 173, 284, 350, 383, 632, 2774, 2837
CviQI GTAC 7 cut(s) 173, 284, 350, 383, 632, 2774, 2837
DdeI CTNAG 8 cut(s) 1386, 1424, 1754, 1878, 2054, 2387, 2673, 2733
DraI TTTAAA 1 cut(s) 3097
DraIII CACNNNGTG 1 cut(s) 1315
EaeI YGGCCR 1 cut(s) 2869
Eam1104I CTCTTC 3 cut(s) 622, 839, 2019
EarI CTCTTC 3 cut(s) 622, 839, 2019
Eco105I TACGTA 2 cut(s) 353, 386
Eco130I CCWWGG 4 cut(s) 400, 1744, 1980, 2213
Eco31I GGTCTC 4 cut(s) 79, 1299, 1655, 2291
Eco32I GATATC 1 cut(s) 13
Eco47I GGWCC 5 cut(s) 504, 1450, 2197, 2217, 3136
Eco47III AGCGCT 1 cut(s) 1961
Eco57I CTGAAG 6 cut(s) 879, 1007, 1340, 2593, 2678, 3137
Eco81I CCTNAGG 1 cut(s) 2673
Eco88I CYCGRG 1 cut(s) 435
EcoO109I RGGNCCY 1 cut(s) 1463
EcoRI GAATTC 1 cut(s) 1731
EcoRII CCWGG 7 cut(s) 705, 1100, 1270, 2199, 2716, 2994, 3109
EcoRV GATATC 1 cut(s) 13
EcoT14I CCWWGG 4 cut(s) 400, 1744, 1980, 2213
EcoT22I ATGCAT 3 cut(s) 901, 1215, 2560
ErhI CCWWGG 4 cut(s) 400, 1744, 1980, 2213
FalI AAGNNNNNCTT 6 cut(s) 525, 557, 528, 560, 2683, 2715
FaqI GGGAC 3 cut(s) 143, 2026, 2908
FauI CCCGC 3 cut(s) 341, 3041, 3125
FauNDI CATATG 1 cut(s) 757
FblI GTMKAC 3 cut(s) 24, 266, 560
Fnu4HI GCNGC 5 cut(s) 478, 908, 2359, 2632, 3051
Fsp4HI GCNGC 5 cut(s) 478, 908, 2359, 2632, 3051
FspBI CTAG 3 cut(s) 521, 1043, 2229
GlaI GCGC 1 cut(s) 1961
GluI GCNGC 5 cut(s) 478, 908, 2359, 2632, 3051
GsaI CCCAGC 1 cut(s) 1240
HaeII RGCGCY 1 cut(s) 1963
HaeIII GGCC 5 cut(s) 705, 1464, 1971, 2871, 3109
HapII CCGG 2 cut(s) 89, 1397
HhaI GCGC 1 cut(s) 1962
Hin6I GCGC 1 cut(s) 1960
HinP1I GCGC 1 cut(s) 1960
HincII GTYRAC 1 cut(s) 1171
HindII GTYRAC 1 cut(s) 1171
HindIII AAGCTT 3 cut(s) 1760, 2408, 2438
HpaII CCGG 2 cut(s) 89, 1397
HphI GGTGA 5 cut(s) 818, 1369, 2342, 3002, 3133
Hpy166II GTNNAC 9 cut(s) 25, 267, 286, 368, 561, 1171, 1450, 2774, 2984
Hpy8I GTNNAC 9 cut(s) 25, 267, 286, 368, 561, 1171, 1450, 2774, 2984
HpyAV CCTTC 8 cut(s) 745, 826, 1463, 1703, 2230, 2235, 2424, 3112
HpyCH4III ACNGT 9 cut(s) 156, 430, 1133, 1206, 1315, 1787, 2778, 2801, 2852
HpyCH4IV ACGT 5 cut(s) 352, 381, 385, 1279, 1900
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 596, 1968, 2825
HpyF3I CTNAG 8 cut(s) 1386, 1424, 1754, 1878, 2054, 2387, 2673, 2733
HpySE526I ACGT 5 cut(s) 352, 381, 385, 1279, 1900
HspAI GCGC 1 cut(s) 1960
LguI GCTCTTC 1 cut(s) 839
LmnI GCTCC 3 cut(s) 4, 1601, 2336
Lsp1109I GCAGC 5 cut(s) 464, 894, 2345, 2618, 3037
LweI GCATC 7 cut(s) 154, 1200, 2057, 2430, 2545, 2567, 2884
MaeI CTAG 3 cut(s) 521, 1043, 2229
MaeII ACGT 5 cut(s) 352, 381, 385, 1279, 1900
MaeIII GTNAC 2 cut(s) 94, 2795
MfeI CAATTG 4 cut(s) 668, 1953, 2317, 2602
MflI RGATCY 3 cut(s) 75, 1702, 1872
MhlI GDGCHC 1 cut(s) 2850
MlsI TGGCCA 1 cut(s) 2871
MluNI TGGCCA 1 cut(s) 2871
MlyI GAGTC 4 cut(s) 200, 1265, 1810, 2253
MmeI TCCRAC 2 cut(s) 1218, 1867
Mox20I TGGCCA 1 cut(s) 2871
Mph1103I ATGCAT 3 cut(s) 901, 1215, 2560
MroXI GAANNNNTTC 6 cut(s) 540, 1245, 1344, 1941, 2663, 2700
MscI TGGCCA 1 cut(s) 2871
MslI CAYNNNNRTG 2 cut(s) 456, 1358
Msp20I TGGCCA 1 cut(s) 2871
MspA1I CMGCKG 1 cut(s) 178
MspCI CTTAAG 3 cut(s) 1865, 1963, 2435
MspI CCGG 2 cut(s) 89, 1397
MspR9I CCNGG 7 cut(s) 707, 1102, 1272, 2201, 2718, 2996, 3111
MunI CAATTG 4 cut(s) 668, 1953, 2317, 2602
Mva1269I GAATGC 4 cut(s) 336, 411, 558, 2692
MvaI CCWGG 7 cut(s) 707, 1102, 1272, 2201, 2718, 2996, 3111
MvnI CGCG 1 cut(s) 3036
MwoI GCNNNNNNNGC 4 cut(s) 52, 596, 1968, 2825
NcoI CCATGG 2 cut(s) 400, 2213
NdeI CATATG 1 cut(s) 757
NlaIV GGNNCC 3 cut(s) 934, 1465, 3137
NmeAIII GCCGAG 1 cut(s) 1277
NsiI ATGCAT 3 cut(s) 901, 1215, 2560
NspI RCATGY 4 cut(s) 903, 1083, 2548, 2923
NspV TTCGAA 1 cut(s) 1441
PaeI GCATGC 1 cut(s) 1083
PagI TCATGA 4 cut(s) 340, 691, 1297, 1797
PciSI GCTCTTC 1 cut(s) 839
PctI GAATGC 4 cut(s) 336, 411, 558, 2692
PdmI GAANNNNTTC 6 cut(s) 540, 1245, 1344, 1941, 2663, 2700
PfeI GAWTC 8 cut(s) 329, 396, 809, 1509, 1541, 1774, 1826, 2383
Pfl23II CGTACG 1 cut(s) 382
PflMI CCANNNNNTGG 1 cut(s) 1745
PfoI TCCNGGA 2 cut(s) 2199, 2716
PkrI GCNGC 5 cut(s) 479, 909, 2360, 2633, 3052
PleI GAGTC 4 cut(s) 200, 1264, 1809, 2252
PpsI GAGTC 4 cut(s) 200, 1264, 1809, 2252
Ppu21I YACGTR 2 cut(s) 353, 386
PshAI GACNNNNGTC 1 cut(s) 1131
PshBI ATTAAT 1 cut(s) 2028
PsiI TTATAA 1 cut(s) 1089
Psp1406I AACGTT 1 cut(s) 1900
Psp6I CCWGG 7 cut(s) 705, 1100, 1270, 2199, 2716, 2994, 3109
PspFI CCCAGC 1 cut(s) 1236
PspGI CCWGG 7 cut(s) 705, 1100, 1270, 2199, 2716, 2994, 3109
PspLI CGTACG 1 cut(s) 382
PspN4I GGNNCC 3 cut(s) 934, 1465, 3137
PspPI GGNCC 7 cut(s) 504, 1450, 1463, 2197, 2217, 3107, 3136
PstI CTGCAG 2 cut(s) 2636, 3055
PstNI CAGNNNCTG 1 cut(s) 596
PsuI RGATCY 3 cut(s) 75, 1702, 1872
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 7 cut(s) 174, 285, 351, 384, 633, 2775, 2838
RsaNI GTAC 7 cut(s) 173, 284, 350, 383, 632, 2774, 2837
RseI CAYNNNNRTG 2 cut(s) 456, 1358
SapI GCTCTTC 1 cut(s) 839
SatI GCNGC 5 cut(s) 478, 908, 2359, 2632, 3051
Sau96I GGNCC 7 cut(s) 504, 1450, 1463, 2197, 2217, 3107, 3136
ScaI AGTACT 1 cut(s) 633
SchI GAGTC 4 cut(s) 200, 1265, 1810, 2253
ScrFI CCNGG 7 cut(s) 707, 1102, 1272, 2201, 2718, 2996, 3111
SduI GDGCHC 1 cut(s) 2850
SfaNI GCATC 7 cut(s) 154, 1200, 2057, 2430, 2545, 2567, 2884
SfcI CTRYAG 4 cut(s) 561, 2145, 2632, 3051
SfuI TTCGAA 1 cut(s) 1441
SinI GGWCC 5 cut(s) 504, 1450, 2197, 2217, 3136
SmiMI CAYNNNNRTG 2 cut(s) 456, 1358
SmlI CTYRAG 6 cut(s) 920, 1858, 1865, 1963, 2435, 3083
SmoI CTYRAG 6 cut(s) 920, 1858, 1865, 1963, 2435, 3083
SnaBI TACGTA 2 cut(s) 353, 386
SphI GCATGC 1 cut(s) 1083
SsiI CCGC 4 cut(s) 334, 854, 3034, 3132
SspI AATATT 2 cut(s) 1065, 1582
SspMI CTAG 3 cut(s) 521, 1043, 2229
StyD4I CCNGG 7 cut(s) 705, 1100, 1270, 2199, 2716, 2994, 3109
StyI CCWWGG 4 cut(s) 400, 1744, 1980, 2213
TaaI ACNGT 9 cut(s) 156, 430, 1133, 1206, 1315, 1787, 2778, 2801, 2852
TaiI ACGT 5 cut(s) 355, 384, 388, 1282, 1903
TaqI TCGA 9 cut(s) 1014, 1125, 1441, 1497, 1833, 2684, 2742, 2841, 2937
TatI WGTACW 4 cut(s) 283, 631, 2773, 2836
TfiI GAWTC 8 cut(s) 329, 396, 809, 1509, 1541, 1774, 1826, 2383
TscAI CASTG 4 cut(s) 161, 1318, 1394, 2544
TseI GCWGC 5 cut(s) 477, 907, 2358, 2631, 3050
TspRI CASTG 4 cut(s) 161, 1318, 1394, 2544
Van91I CCANNNNNTGG 1 cut(s) 1745
Vha464I CTTAAG 3 cut(s) 1865, 1963, 2435
VpaK11BI GGWCC 5 cut(s) 504, 1450, 2197, 2217, 3136
VspI ATTAAT 1 cut(s) 2028
XapI RAATTY 7 cut(s) 240, 676, 1411, 1437, 1731, 2727, 2949
XbaI TCTAGA 1 cut(s) 2228
XceI RCATGY 4 cut(s) 903, 1083, 2548, 2923
XcmI CCANNNNNNNNNTGG 1 cut(s) 1378
XmiI GTMKAC 3 cut(s) 24, 266, 560
XmnI GAANNNNTTC 6 cut(s) 540, 1245, 1344, 1941, 2663, 2700
XspI CTAG 3 cut(s) 521, 1043, 2229
ZrmI AGTACT 1 cut(s) 633
Zsp2I ATGCAT 3 cut(s) 901, 1215, 2560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.