Rroxscaffold_2G00146640

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000105
Physical Location & Seq
Reverse (-)
84497970 .. 84505116
7147 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00146640.1

Sequence Viewer

Length: 2664 bp
ATGGAAAAAACTGGAAATGTAGAGGATGAATATAATGGTGCTGCTATGATACAAAAATGCCTGTGTAGAATAAGGGTGCTTCTTGTTCTTGATGATGTGGATCAATTGACACAATTAGAAAAACTAGCTGGACATCCTAACTGGTTCGGTCCGGGCAGTAGAGTTATCATAACAAGCACAGATATTCATCTGCTGAACATGCATGGTGTTGATGCTGTCTATCAGGCTACTGTGTTAGATCCTAATGAAGCACTTCAACTTATGAGTTTAAAGGCTTTTAAGAAAATTCCCCCGCCTGAAAATTATTTGGAGCTATGCCACAGCATTTTAACCTATGCTCATGGACTTCCCTTGGCTCTTGTGGTTATGGGTTGCTTTCTGTATGGTAAAAGCACCAATGAATGGAGAAGTGCAATAGATATGCTCAAGAAAACACCTGAAAAAAGTATCACTGATGTGCTTCATATAAGTTTTGATGGACTAAGGGAAATAGAAAAAGAAGTATTCCTACATATTGCTTGTTTTTATAAGGGCAAGGATAGGAATCGTGTGACAGAAATACTAGACTATTGTCAGCTTCACCCTGATATTAATTTAAGAGTTCTTGTTGATAAATCTCTCATTACTATTTCAAACAACGAAATCTGCATGCATGATTTGCTACAAGAAATGGGAAGGGAAATAGTTCGTCAAGAGTCCCCTAAAGAGCCAGGCAAACGTAGTAGACTATGGTCACATGAAGACATACACAACGTGCTGAAGAAAAATGAGGGAACAGATGCAATTCAAGGCATTGTAATGGACTTGCCTGAATTAGAAATGGCTCATTGGCACCCAGAAGCCTTCTCAAATTTGTCTAAACTTACTCTTCTCCATATTCATAATGTCAATCTCTCGAAGGGCCTCACTTGTCTTTCTAGCTCCTTGAGACTTGTGGAATGGATTGGGTACCCCTTAAGATCTCTCCCACAAAATTTCAAACCAGATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTGATCAGCTTTGGAAGGGAAAACAGTGTTTGGACAAGTTGAAGCTCCTCAAACTCTGCCATTCCCATAACATTGTCAAAACGCCAGACTTCACGGGCGTTCAAAATCTTGAGACATTAGATCTTGAAGGATGCATAAATCTGACACGAGTTCATCAATCCCTTCGATTTCTCAAGAGGCTTGTTGTACTGAATCTTAAAGACTGCAAAAGTCTTGAGGGCCTGCCAGGTAAAATCGAATCACAGTCTCTTGAAACTTTTATTCTTTCTGGCTGCTCAAAAGTTAAGAAGATCCCTGAGTTTGTTGAAAATATGAAACACTTGTCGACGCTTTGTCTCGACAAGACTGCCATAAAAGAACTACCTTCCTCAATTGAACACCTCAGTGGCCTTGCTTCTTTGGATCTAAGCAACTGCAAAGACCTTGTTTCACTTCCGAGCACAATTCATTCGTTGAAGTCTCTTAAAAAGCTGAATCTTTCTGGATGCTTGAAACTTGGCCAAAGCCATGCCAGTGTAACAGAATGGAAGGATCTTTTAAGACACGGTTGTGAAGTGGTTTGGGAATATTTCTCATGGTCCTTGTCTTCTGTATGGATGCAAAGCGACGATACAGAACCAACTAGTATGCCGTTGTCTGTATCTGATCTGTGTAGCTCTACAGAGCAAAGAATGAACAGATCACTGAGCATGCGGTTGCCTTTATTTGGTCTTTGTAATTTAACAGATCTAAACCTAAGTAACTGCAATCTTGGTGATACAGCATTTGCCAACAACTTCGGCTACTTTCCCTCTTTACTGGCATTAAATCTCAGTGGAAATAATTTTTCTAAGCTTCCCCCAGGCATCAGATCATGTTTGAAACTAGAGAAGATTAACTTGGAGAACTGCATGAGTCTTCAAGACTTGTCTGGCCTTCCTTCAAATAGTACATTGGATATAAGAGCAGATGGTTGTTCTTCACTGGAAATGTTGCTTCATGCATTCAATTTTGATAGATTGAACATATCATATTTGAATTTCATCAACTGCTTCAAAATAAAGGACAATCAAGGCTGCAATAGCATGTCATTTGAAATGCTGAAGTTATTCCTTTGCCAGGGCATCTCTAATGCAAAGCAAACTTTTCAAGTTGTACTTCCTGGAAGGAAAATTCCCAGCTGGTTCAGCCATGGAAGGCCTGGAGTTTCGTTAAGTCATAGGCAAAGGCCAGATTTCGATTTCTGGGATACCAAATCTTTGATGGGTTTTGCTCTCTGTGTTGTGTTTAGACTCCATGAGCACCATCACATTAATGAACTCAACACAGATCAATTCAAGACATTTAAAGCTACACATCATCTGGTATGTTGCCTGAAAGTCAATGGAAGAGAACTAGAAGTGTGTGGCAGACAACCTGCGTTTCGCTTTAGTGAAGAATTTTGCCGGGTTGAGTCGCATCACATTTGGCTATTCTATGTTCCCTGCGATTACTTTGGTACAGAGTGGAAGACTAGCAATCATATTGAGTTCTTATTTGAAACCAAAGGGCCTGGCCTGAAGGTGAAAAGATGTGGTGTCCGTCCGATATATGAACAAGATGTGCAAGAGATGATCAAAACAATGAATCACTCCAACAGCAGGATTGAAGCACTTTCACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

887

Amino Acids

100.82

Weight (kDa)

6.76

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 10 - 95 9.2e-08 NB-ARC domain
WHD_ROQ1 PF23282 164 - 233 1.5e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 394 - 503 5.5e-10 Leucine-rich repeat region
LRR_13 PF23286 423 - 510 3.6e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 720 - 869 1.1e-10 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 528
Acc36I ACCTGC 1 cut(s) 2425
Acc65I GGTACC 1 cut(s) 948
AccB1I GGYRCC 2 cut(s) 831, 948
AccB7I CCANNNNNTGG 1 cut(s) 402
AccI GTMKAC 2 cut(s) 724, 1346
AciI CCGC 2 cut(s) 293, 1714
AclWI GGATC 5 cut(s) 108, 233, 1306, 1431, 1560
AcoI YGGCCR 1 cut(s) 1519
AcsI RAATTY 6 cut(s) 285, 850, 973, 2038, 2172, 2438
AcuI CTGAAG 3 cut(s) 779, 2123, 2579
AdeI CACNNNGTG 1 cut(s) 754
AfaI GTAC 5 cut(s) 950, 1209, 1951, 2157, 2500
AfiI CCNNNNNNNGG 4 cut(s) 402, 1727, 2119, 2640
AflII CTTAAG 1 cut(s) 955
AhdI GACNNNNNGTC 1 cut(s) 1353
AhlI ACTAGT 1 cut(s) 1643
AjnI CCWGG 7 cut(s) 709, 1246, 1861, 2118, 2161, 2200, 2551
AjuI GAANNNNNNNTTGG 2 cut(s) 1883, 1915
AleI CACNNNNGTG 3 cut(s) 455, 1404, 1569
Alw21I GWGCWC 2 cut(s) 1463, 2304
Alw26I GTCTC 5 cut(s) 922, 1127, 1272, 1361, 1485
AlwI GGATC 5 cut(s) 108, 233, 1306, 1431, 1560
AoxI GGCC 9 cut(s) 901, 1240, 1408, 1519, 1933, 2198, 2228, 2549, 2554
ApeKI GCWGC 3 cut(s) 41, 1293, 2076
ApoI RAATTY 6 cut(s) 285, 850, 973, 2038, 2172, 2438
AseI ATTAAT 2 cut(s) 591, 2313
Asp700I GAANNNNTTC 3 cut(s) 252, 684, 2108
Asp718I GGTACC 1 cut(s) 948
AspS9I GGNCC 5 cut(s) 149, 901, 1240, 1599, 2549
AsuC2I CCSGG 2 cut(s) 153, 2447
AsuHPI GGTGA 4 cut(s) 572, 1787, 2575, 2649
AvaII GGWCC 2 cut(s) 149, 1599
BalI TGGCCA 1 cut(s) 1521
BanI GGYRCC 2 cut(s) 831, 948
BarI GAAGNNNNNNTAC 2 cut(s) 492, 524
BauI CACGAG 1 cut(s) 1167
BbsI GAAGAC 4 cut(s) 747, 1599, 1910, 2516
Bbv12I GWGCWC 2 cut(s) 1463, 2304
BbvI GCAGC 3 cut(s) 28, 1280, 2063
BccI CCATC 4 cut(s) 470, 1964, 2257, 2313
BceAI ACGGC 1 cut(s) 1636
BcgI CGANNNNNNTGC 2 cut(s) 1349, 1383
BciT130I CCWGG 7 cut(s) 711, 1248, 1863, 2120, 2163, 2202, 2553
BciVI GTATCC 1 cut(s) 2242
BclI TGATCA 2 cut(s) 1024, 2613
BcnI CCSGG 2 cut(s) 153, 2447
BcoDI GTCTC 5 cut(s) 922, 1127, 1272, 1361, 1485
BcuI ACTAGT 1 cut(s) 1643
BfaI CTAG 7 cut(s) 125, 563, 918, 1644, 1886, 2396, 2514
BfmI CTRYAG 1 cut(s) 1680
BfrI CTTAAG 1 cut(s) 955
BfuAI ACCTGC 1 cut(s) 2425
BfuI GTATCC 1 cut(s) 2242
BglII AGATCT 3 cut(s) 959, 1141, 1747
BisI GCNGC 3 cut(s) 42, 1294, 2077
BlsI GCNGC 3 cut(s) 43, 1295, 2078
Bme1390I CCNGG 9 cut(s) 153, 711, 1248, 1863, 2120, 2163, 2202, 2447, 2553
Bme18I GGWCC 2 cut(s) 149, 1599
BmeRI GACNNNNNGTC 1 cut(s) 1353
BmgT120I GGNCC 5 cut(s) 149, 901, 1240, 1599, 2549
BmiI GGNNCC 2 cut(s) 833, 950
BmrFI CCNGG 9 cut(s) 153, 711, 1248, 1863, 2120, 2163, 2202, 2447, 2553
BmsI GCATC 8 cut(s) 202, 769, 1142, 1496, 1608, 1875, 2133, 2467
BoxI GACNNNNGTC 2 cut(s) 570, 730
BpiI GAAGAC 4 cut(s) 747, 1599, 1910, 2516
BpmI CTGGAG 1 cut(s) 2223
BpuEI CTTGAG 5 cut(s) 410, 946, 1151, 1178, 1256
BpuMI CCSGG 2 cut(s) 153, 2447
BsaBI GATNNNNATC 3 cut(s) 99, 186, 543
BsaJI CCNNGG 4 cut(s) 351, 1861, 2119, 2191
Bsc4I CCNNNNNNNGG 4 cut(s) 402, 1727, 2119, 2640
Bse1I ACTGG 5 cut(s) 16, 146, 1533, 1824, 1989
Bse8I GATNNNNATC 3 cut(s) 99, 186, 543
BseBI CCWGG 7 cut(s) 711, 1248, 1863, 2120, 2163, 2202, 2553
BseDI CCNNGG 4 cut(s) 351, 1861, 2119, 2191
BseGI GGATG 5 cut(s) 31, 133, 1157, 1511, 1623
BseJI GATNNNNATC 3 cut(s) 99, 186, 543
BseLI CCNNNNNNNGG 4 cut(s) 402, 1727, 2119, 2640
BseMII CTCAG 4 cut(s) 1308, 1417, 1697, 1846
BseNI ACTGG 5 cut(s) 16, 146, 1533, 1824, 1989
BseRI GAGGAG 1 cut(s) 1058
BseXI GCAGC 3 cut(s) 28, 1280, 2063
BseYI CCCAGC 1 cut(s) 2177
BshFI GGCC 9 cut(s) 903, 1242, 1410, 1521, 1935, 2200, 2230, 2551, 2556
BshNI GGYRCC 2 cut(s) 831, 948
BsiHKAI GWGCWC 2 cut(s) 1463, 2304
BsiSI CCGG 2 cut(s) 152, 2446
BslFI GGGAC 1 cut(s) 682
BslI CCNNNNNNNGG 4 cut(s) 402, 1727, 2119, 2640
BsmAI GTCTC 5 cut(s) 922, 1127, 1272, 1361, 1485
BsmFI GGGAC 1 cut(s) 682
BsmI GAATGC 1 cut(s) 2003
BsnI GGCC 9 cut(s) 903, 1242, 1410, 1521, 1935, 2200, 2230, 2551, 2556
Bsp1286I GDGCHC 2 cut(s) 1463, 2304
Bsp19I CCATGG 1 cut(s) 2191
BspACI CCGC 2 cut(s) 293, 1714
BspANI GGCC 9 cut(s) 903, 1242, 1410, 1521, 1935, 2200, 2230, 2551, 2556
BspCNI CTCAG 4 cut(s) 1309, 1416, 1698, 1845
BspLI GGNNCC 2 cut(s) 833, 950
BspMI ACCTGC 1 cut(s) 2425
BspPI GGATC 5 cut(s) 108, 233, 1306, 1431, 1560
BspT107I GGYRCC 2 cut(s) 831, 948
BspTI CTTAAG 1 cut(s) 955
BsrI ACTGG 5 cut(s) 16, 146, 1533, 1824, 1989
BssECI CCNNGG 4 cut(s) 351, 1861, 2119, 2191
BssSI CACGAG 1 cut(s) 1167
BssT1I CCWWGG 2 cut(s) 351, 2191
Bst2BI CACGAG 1 cut(s) 1167
Bst2UI CCWGG 7 cut(s) 711, 1248, 1863, 2120, 2163, 2202, 2553
Bst4CI ACNGT 4 cut(s) 232, 1047, 1266, 1568
Bst6I CTCTTC 2 cut(s) 873, 2383
BstAFI CTTAAG 1 cut(s) 955
BstAPI GCANNNNNTGC 1 cut(s) 658
BstC8I GCNNGC 3 cut(s) 650, 1244, 1712
BstDEI CTNAG 8 cut(s) 482, 1317, 1403, 1427, 1706, 1757, 1832, 1851
BstDSI CCRYGG 1 cut(s) 2191
BstENI CCTNNNNNAGG 1 cut(s) 2117
BstF5I GGATG 5 cut(s) 31, 133, 1157, 1511, 1623
BstMAI GTCTC 5 cut(s) 922, 1127, 1272, 1361, 1485
BstMWI GCNNNNNNNGC 4 cut(s) 199, 658, 2082, 2187
BstNI CCWGG 7 cut(s) 711, 1248, 1863, 2120, 2163, 2202, 2553
BstNSI RCATGY 4 cut(s) 202, 652, 1714, 2089
BstPAI GACNNNNGTC 2 cut(s) 570, 730
BstSCI CCNGG 9 cut(s) 151, 709, 1246, 1861, 2118, 2161, 2200, 2445, 2551
BstSFI CTRYAG 1 cut(s) 1680
BstV1I GCAGC 3 cut(s) 28, 1280, 2063
BstV2I GAAGAC 4 cut(s) 747, 1599, 1910, 2516
BstX2I RGATCY 7 cut(s) 238, 959, 1141, 1311, 1423, 1552, 1747
BstYI RGATCY 7 cut(s) 238, 959, 1141, 1311, 1423, 1552, 1747
BsuI GTATCC 1 cut(s) 2242
BsuRI GGCC 9 cut(s) 903, 1242, 1410, 1521, 1935, 2200, 2230, 2551, 2556
BtgI CCRYGG 1 cut(s) 2191
BtsCI GGATG 5 cut(s) 31, 133, 1157, 1511, 1623
BtsIMutI CAGTG 7 cut(s) 450, 1052, 1411, 1540, 1703, 1840, 1982
BveI ACCTGC 1 cut(s) 2425
Cac8I GCNNGC 3 cut(s) 650, 1244, 1712
Cfr13I GGNCC 5 cut(s) 149, 901, 1240, 1599, 2549
CpoI CGGWCCG 1 cut(s) 149
CseI GACGC 1 cut(s) 1357
Csp6I GTAC 5 cut(s) 949, 1208, 1950, 2156, 2499
CspI CGGWCCG 1 cut(s) 149
CviQI GTAC 5 cut(s) 949, 1208, 1950, 2156, 2499
DdeI CTNAG 8 cut(s) 482, 1317, 1403, 1427, 1706, 1757, 1832, 1851
DraI TTTAAA 2 cut(s) 270, 2347
DraIII CACNNNGTG 1 cut(s) 754
DriI GACNNNNNGTC 1 cut(s) 1353
EaeI YGGCCR 1 cut(s) 1519
Eam1104I CTCTTC 2 cut(s) 873, 2383
Eam1105I GACNNNNNGTC 1 cut(s) 1353
EarI CTCTTC 2 cut(s) 873, 2383
Eco130I CCWWGG 2 cut(s) 351, 2191
Eco147I AGGCCT 1 cut(s) 2200
Eco47I GGWCC 2 cut(s) 149, 1599
Eco57I CTGAAG 3 cut(s) 779, 2123, 2579
EcoNI CCTNNNNNAGG 1 cut(s) 2117
EcoO109I RGGNCCY 3 cut(s) 901, 1240, 2549
EcoRII CCWGG 7 cut(s) 709, 1246, 1861, 2118, 2161, 2200, 2551
EcoT14I CCWWGG 2 cut(s) 351, 2191
EcoT22I ATGCAT 4 cut(s) 204, 654, 1157, 2005
ErhI CCWWGG 2 cut(s) 351, 2191
FalI AAGNNNNNCTT 4 cut(s) 1883, 1915, 2142, 2174
FaqI GGGAC 1 cut(s) 682
FauI CCCGC 1 cut(s) 300
FbaI TGATCA 2 cut(s) 1024, 2613
FblI GTMKAC 2 cut(s) 724, 1346
Fnu4HI GCNGC 3 cut(s) 42, 1294, 2077
FokI GGATG 5 cut(s) 38, 120, 1164, 1518, 1630
Fsp4HI GCNGC 3 cut(s) 42, 1294, 2077
FspBI CTAG 7 cut(s) 125, 563, 918, 1644, 1886, 2396, 2514
GluI GCNGC 3 cut(s) 42, 1294, 2077
GsaI CCCAGC 1 cut(s) 2181
GsuI CTGGAG 1 cut(s) 2223
HaeIII GGCC 9 cut(s) 903, 1242, 1410, 1521, 1935, 2200, 2230, 2551, 2556
HapII CCGG 2 cut(s) 152, 2446
HgaI GACGC 1 cut(s) 1357
HincII GTYRAC 1 cut(s) 1347
HindII GTYRAC 1 cut(s) 1347
HindIII AAGCTT 1 cut(s) 1853
HinfI GANTC 9 cut(s) 544, 695, 1213, 1259, 1495, 1915, 2292, 2453, 2626
HpaII CCGG 2 cut(s) 152, 2446
HphI GGTGA 4 cut(s) 572, 1787, 2575, 2649
Hpy166II GTNNAC 2 cut(s) 725, 1347
Hpy188I TCNGA 5 cut(s) 1164, 1458, 1666, 1871, 2586
Hpy8I GTNNAC 2 cut(s) 725, 1347
Hpy99I CGWCG 2 cut(s) 1351, 1631
HpyCH4III ACNGT 4 cut(s) 232, 1047, 1266, 1568
HpyCH4IV ACGT 2 cut(s) 718, 753
HpyF10VI GCNNNNNNNGC 4 cut(s) 199, 658, 2082, 2187
HpyF3I CTNAG 8 cut(s) 482, 1317, 1403, 1427, 1706, 1757, 1832, 1851
HpySE526I ACGT 2 cut(s) 718, 753
KpnI GGTACC 1 cut(s) 952
Ksp22I TGATCA 2 cut(s) 1024, 2613
LmnI GCTCC 3 cut(s) 310, 926, 1071
Lsp1109I GCAGC 3 cut(s) 28, 1280, 2063
LweI GCATC 8 cut(s) 202, 769, 1142, 1496, 1608, 1875, 2133, 2467
MaeI CTAG 7 cut(s) 125, 563, 918, 1644, 1886, 2396, 2514
MaeII ACGT 2 cut(s) 718, 753
MaeIII GTNAC 4 cut(s) 550, 732, 1537, 1760
MfeI CAATTG 2 cut(s) 104, 1392
MflI RGATCY 7 cut(s) 238, 959, 1141, 1311, 1423, 1552, 1747
MhlI GDGCHC 2 cut(s) 1463, 2304
MlsI TGGCCA 1 cut(s) 1521
MluNI TGGCCA 1 cut(s) 1521
MlyI GAGTC 4 cut(s) 704, 1924, 2286, 2462
MmeI TCCRAC 1 cut(s) 2658
MnlI CCTC 9 cut(s) 16, 763, 914, 1079, 1191, 1231, 1399, 1412, 1822
Mox20I TGGCCA 1 cut(s) 1521
Mph1103I ATGCAT 4 cut(s) 204, 654, 1157, 2005
MroXI GAANNNNTTC 3 cut(s) 252, 684, 2108
MscI TGGCCA 1 cut(s) 1521
MslI CAYNNNNRTG 6 cut(s) 455, 797, 1404, 1533, 1569, 2313
Msp20I TGGCCA 1 cut(s) 1521
MspA1I CMGCKG 1 cut(s) 2181
MspCI CTTAAG 1 cut(s) 955
MspI CCGG 2 cut(s) 152, 2446
MspR9I CCNGG 9 cut(s) 153, 711, 1248, 1863, 2120, 2163, 2202, 2447, 2553
MunI CAATTG 2 cut(s) 104, 1392
Mva1269I GAATGC 1 cut(s) 2003
MvaI CCWGG 7 cut(s) 711, 1248, 1863, 2120, 2163, 2202, 2553
MwoI GCNNNNNNNGC 4 cut(s) 199, 658, 2082, 2187
NciI CCSGG 2 cut(s) 153, 2447
NcoI CCATGG 1 cut(s) 2191
NlaIV GGNNCC 2 cut(s) 833, 950
NmuCI GTSAC 2 cut(s) 550, 732
NsiI ATGCAT 4 cut(s) 204, 654, 1157, 2005
NspI RCATGY 4 cut(s) 202, 652, 1714, 2089
OliI CACNNNNGTG 3 cut(s) 455, 1404, 1569
PaeI GCATGC 2 cut(s) 652, 1714
PceI AGGCCT 1 cut(s) 2200
PctI GAATGC 1 cut(s) 2003
PdmI GAANNNNTTC 3 cut(s) 252, 684, 2108
PfeI GAWTC 5 cut(s) 544, 1213, 1259, 1495, 2626
PflMI CCANNNNNTGG 1 cut(s) 402
PfoI TCCNGGA 1 cut(s) 2161
PkrI GCNGC 3 cut(s) 43, 1295, 2078
PleI GAGTC 4 cut(s) 703, 1923, 2286, 2461
PpsI GAGTC 4 cut(s) 703, 1923, 2286, 2461
PshAI GACNNNNGTC 2 cut(s) 570, 730
PshBI ATTAAT 2 cut(s) 591, 2313
PsiI TTATAA 1 cut(s) 528
Psp6I CCWGG 7 cut(s) 709, 1246, 1861, 2118, 2161, 2200, 2551
PspFI CCCAGC 1 cut(s) 2177
PspGI CCWGG 7 cut(s) 709, 1246, 1861, 2118, 2161, 2200, 2551
PspN4I GGNNCC 2 cut(s) 833, 950
PspPI GGNCC 5 cut(s) 149, 901, 1240, 1599, 2549
PsuI RGATCY 7 cut(s) 238, 959, 1141, 1311, 1423, 1552, 1747
PvuII CAGCTG 1 cut(s) 2181
RsaI GTAC 5 cut(s) 950, 1209, 1951, 2157, 2500
RsaNI GTAC 5 cut(s) 949, 1208, 1950, 2156, 2499
RseI CAYNNNNRTG 6 cut(s) 455, 797, 1404, 1533, 1569, 2313
Rsr2I CGGWCCG 1 cut(s) 149
RsrII CGGWCCG 1 cut(s) 149
SalI GTCGAC 1 cut(s) 1345
SatI GCNGC 3 cut(s) 42, 1294, 2077
Sau96I GGNCC 5 cut(s) 149, 901, 1240, 1599, 2549
SchI GAGTC 4 cut(s) 704, 1924, 2286, 2462
ScrFI CCNGG 9 cut(s) 153, 711, 1248, 1863, 2120, 2163, 2202, 2447, 2553
SduI GDGCHC 2 cut(s) 1463, 2304
SfaNI GCATC 8 cut(s) 202, 769, 1142, 1496, 1608, 1875, 2133, 2467
SfcI CTRYAG 1 cut(s) 1680
SinI GGWCC 2 cut(s) 149, 1599
SmiMI CAYNNNNRTG 6 cut(s) 455, 797, 1404, 1533, 1569, 2313
SmlI CTYRAG 6 cut(s) 425, 925, 955, 1130, 1193, 1235
SmoI CTYRAG 6 cut(s) 425, 925, 955, 1130, 1193, 1235
SpeI ACTAGT 1 cut(s) 1643
SphI GCATGC 2 cut(s) 652, 1714
SseBI AGGCCT 1 cut(s) 2200
SsiI CCGC 2 cut(s) 293, 1714
SspI AATATT 1 cut(s) 1589
SspMI CTAG 7 cut(s) 125, 563, 918, 1644, 1886, 2396, 2514
StuI AGGCCT 1 cut(s) 2200
StyD4I CCNGG 9 cut(s) 151, 709, 1246, 1861, 2118, 2161, 2200, 2445, 2551
StyI CCWWGG 2 cut(s) 351, 2191
TaaI ACNGT 4 cut(s) 232, 1047, 1266, 1568
TaiI ACGT 2 cut(s) 721, 756
TaqI TCGA 6 cut(s) 896, 1186, 1257, 1346, 1359, 2238
TaqII GACCGA 1 cut(s) 137
TatI WGTACW 3 cut(s) 1207, 1949, 2155
TfiI GAWTC 5 cut(s) 544, 1213, 1259, 1495, 2626
TscAI CASTG 7 cut(s) 457, 1052, 1411, 1540, 1710, 1840, 1989
TseFI GTSAC 2 cut(s) 550, 732
TseI GCWGC 3 cut(s) 41, 1293, 2076
Tsp45I GTSAC 2 cut(s) 550, 732
TspGWI ACGGA 1 cut(s) 2570
TspRI CASTG 7 cut(s) 457, 1052, 1411, 1540, 1710, 1840, 1989
Van91I CCANNNNNTGG 1 cut(s) 402
Vha464I CTTAAG 1 cut(s) 955
VpaK11BI GGWCC 2 cut(s) 149, 1599
VspI ATTAAT 2 cut(s) 591, 2313
XagI CCTNNNNNAGG 1 cut(s) 2117
XapI RAATTY 6 cut(s) 285, 850, 973, 2038, 2172, 2438
XceI RCATGY 4 cut(s) 202, 652, 1714, 2089
XcmI CCANNNNNNNNNTGG 2 cut(s) 2198, 2260
XmiI GTMKAC 2 cut(s) 724, 1346
XmnI GAANNNNTTC 3 cut(s) 252, 684, 2108
XspI CTAG 7 cut(s) 125, 563, 918, 1644, 1886, 2396, 2514
Zsp2I ATGCAT 4 cut(s) 204, 654, 1157, 2005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.