Rroxscaffold_4G00327190

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Forward (+)
59690851 .. 59695897
5047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00327190.1

Sequence Viewer

Length: 3705 bp
ATGGAAATACGATGTCTTTCTCAATTTCAGGGGGAGGATACCCGCTATGGTATTACCGATCATCTGTATACTGCACTAGCTCAGAGAGGAATTATCACCTTTCGGGATGACCCATATCTTGATAGAGGAAAATCCATTAGGCCTGAACTTTTAGCTGCAATTGAGGACTCTAGATGTGCGATTGTCATTCTCTCACGGAACTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGCTGGGCCAACAAGTCCTCCCCGTCTTCTTCTGGGTGGATCCTTCTGATGTGCGGCACCAAACGGGAAGTTTTGAGCTCAAAAGGAAAACCCAAGTAGAAGTAACGGAACATGAAGAAGTTCATGGGAAGAATGAGGACAGACTAAATGCCTGGAGAGCTGCTTTGACACAGGTCGCCAATCTTTCTGGCTGGGATTTTACGGGTGGATATCCAACAAACCTAATTGACGAAATTGTTAATCACATACTAAAGAAGTCCGTGTATACATCTTCAAGAGTTGACAAGGGCTTCATAGGAATGAATTCCCGCATTGATGATTTATTAAGCAATTACCTATGTCCACAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATGAAATTTGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAACCAATGGCCTGGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGGCAAAATTTGAAAATATAGAGGATGAATACACAGGAGCTGTTATGATACAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATGAGTTGACACAATTAGAGCAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGACTAGAATTATCATAACCACCACAGATATTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTAATGGGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATGTCCCCCACCAGAAGATTATTTGAATCTGTGCGGTAATATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGTGCTGATGAATGGGCAAGTGCAATAGATAGGCTAAGGAACACACCACCTAAACGAATTATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATCGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTGAGTGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAAAGAACTGTGGATGCATGATTTGCTACAAGAATTGGGCTGGGAAATTGTTCGTGAACAGTCTCCCAAAGAGCCAGGCAAACGAAGTAGATTATGGTCTCATGAAGAGGTCAACAATGTGCTGAAGAAAAATAAGGGAACAGATTCAATTCAAGGCATGGTAATGGAGTTGACTAAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTTGTAATGTGGACCTTCCCAACGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTGAGATCTCTCCCACAAAATTTTGAAGCAGATGAACTTATGGAACTTAACTTGTGCTACAGCAACATTGAACAGCTTTGGAAGGGAACTAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAGCATTGTGGAGACCCCAGACCTCGCAGGCGTTCCGAATCTTGAAACTTTAGATGTTGAAGGATGTGAGAATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGGTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTTGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAATGGCTGAGTGGCCTTGTGTCATTGAATCTAACCAACTGCAAAAATCTTGTTTGTCTTCCAAGCACCATCAATAAGTTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTAAATGTGGGGGTGATGGAGTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGTCATTTACCCATGATCACAGAAAATATGTGAGAGGTTCAATCTTTCATCGATGCAAAGTAGTTTGGCGATCTTTAAAAAAGGTCTTGCCTTCTGGATTGGTGCAACAAGTGAATACAGAGCCAAAGAGTTTCCACTTGCCTATATCACAAAAAGTGAATACAGAGCCAAAGAGTTTCCACTTGCCTATGTCACAAACAGTCCACTTGCCTATGTCACAAACAGTGAATACAGAGCCAAAGAGTTTCCACTTGCCTATATCACAAAAAGTGAATACAGAGCCAATGAGTTTCTGCTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTTGTGAAGGAGCATTTGCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGGAACAATTTTGTTTGTCTTCCTACAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAACAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTAAGTGGTTCAGCCATCAGAGTGATGGGTGTTCTGTAAGTGTATCTCTACCTGCACATTGGAATAACAGTCGGGTTTTGGGATTTGCTCTGTGTGCTGTTTTTGTACTCCATGAGCATCTGGTGGATAAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTACCTGAAGCTCGATGGAAGAGAATTGGACGTATTTGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCAAGGTTAAGTCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTCGGTACAGAGTGGTGGCATAACAGTTGCAGTCAGTTTGAGTTCTTATTTGAAACCAGAGGGCCAGGTCTGACGGTGATGGAGTGTGGAGTCCGTCTGATATATGAGCAAGATGTGCAAGAGTTGAACCAAACAACCACTCAATCAAGCAGTGGGATGTCTCCTTATGAGGATATATTGATTGGTTTTGACATTCCAGTTGCAGGGGAAACCAGTGGCAGTGGTAGCAGAATTTGCACGCTTGAAGAATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1234

Amino Acids

139.66

Weight (kDa)

5.92

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 7 - 177 2.8e-43 TIR domain
TIR_2 PF13676 18 - 100 2.7e-10 TIR domain
NB-ARC PF00931 205 - 359 9.5e-19 NB-ARC domain
NACHT PF05729 209 - 306 1.8e-07 NACHT domain
WHD_ROQ1 PF23282 426 - 497 2.5e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 686 - 771 8.6e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 697 - 765 1.6e-06 Leucine-rich repeat region
C-JID PF20160 1042 - 1190 1.1e-19 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 719, 3202
AccB1I GGYRCC 1 cut(s) 315
AccB7I CCANNNNNTGG 1 cut(s) 608
AccI GTMKAC 3 cut(s) 68, 524, 2917
AciI CCGC 6 cut(s) 43, 313, 568, 643, 850, 1110
AclI AACGTT 1 cut(s) 2131
AclWI GGATC 5 cut(s) 293, 306, 625, 638, 1344
AcuI CTGAAG 3 cut(s) 1571, 3089, 3350
AfaI GTAC 3 cut(s) 2944, 3249, 3457
AfiI CCNNNNNNNGG 5 cut(s) 608, 1382, 2114, 2693, 3653
AjnI CCWGG 6 cut(s) 410, 753, 943, 1501, 3127, 3514
AjuI GAANNNNNNNTTGG 2 cut(s) 2849, 2881
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 9 cut(s) 1494, 1530, 1653, 1714, 1886, 2030, 2150, 2875, 3615
AlwI GGATC 5 cut(s) 293, 306, 625, 638, 1344
AlwNI CAGNNNCTG 1 cut(s) 833
Ama87I CYCGRG 3 cut(s) 669, 2027, 2108
AoxI GGCC 6 cut(s) 140, 265, 751, 1693, 2200, 3512
ApeKI GCWGC 2 cut(s) 155, 419
AseI ATTAAT 1 cut(s) 2072
Asp700I GAANNNNTTC 8 cut(s) 378, 562, 774, 1044, 1476, 1570, 3074, 3111
AspS9I GGNCC 4 cut(s) 265, 941, 1681, 3512
AsuHPI GGTGA 5 cut(s) 88, 2347, 2797, 3407, 3538
AvaI CYCGRG 3 cut(s) 669, 2027, 2108
AvaII GGWCC 2 cut(s) 941, 1681
BamHI GGATCC 2 cut(s) 298, 630
BanI GGYRCC 1 cut(s) 315
BanII GRGCYC 1 cut(s) 339
BauI CACGAG 2 cut(s) 1151, 3438
BbsI GAAGAC 5 cut(s) 277, 1652, 2237, 2813, 3077
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 2 cut(s) 142, 406
BceAI ACGGC 1 cut(s) 1708
BcgI CGANNNNNNTGC 2 cut(s) 2008, 2042
BciT130I CCWGG 6 cut(s) 412, 755, 945, 1503, 3129, 3516
BciVI GTATCC 2 cut(s) 31, 1752
BclI TGATCA 1 cut(s) 2401
BcoDI GTCTC 9 cut(s) 1494, 1530, 1653, 1714, 1886, 2030, 2150, 2875, 3615
BfaI CTAG 5 cut(s) 77, 171, 951, 1355, 2921
BfmI CTRYAG 2 cut(s) 1804, 2826
BfuAI ACCTGC 2 cut(s) 719, 3202
BfuI GTATCC 2 cut(s) 31, 1752
BglII AGATCT 2 cut(s) 1406, 1751
BisI GCNGC 3 cut(s) 156, 314, 420
BlsI GCNGC 3 cut(s) 157, 315, 421
Bme1390I CCNGG 6 cut(s) 412, 755, 945, 1503, 3129, 3516
Bme18I GGWCC 2 cut(s) 941, 1681
BmeT110I CYCGRG 3 cut(s) 669, 2027, 2108
BmgT120I GGNCC 4 cut(s) 265, 941, 1681, 3512
BmiI GGNNCC 3 cut(s) 300, 317, 632
BmrFI CCNGG 6 cut(s) 412, 755, 945, 1503, 3129, 3516
BmrI ACTGGG 1 cut(s) 1746
BmsI GCATC 8 cut(s) 994, 1431, 2288, 2429, 2956, 2978, 3032, 3268
BmuI ACTGGG 1 cut(s) 1746
BoxI GACNNNNGTC 1 cut(s) 431
BpiI GAAGAC 5 cut(s) 277, 1652, 2237, 2813, 3077
BpmI CTGGAG 1 cut(s) 433
Bpu10I CCTNAGC 2 cut(s) 1131, 1217
BpuEI CTTGAG 3 cut(s) 1738, 1768, 2873
Bsa29I ATCGAT 1 cut(s) 2437
BsaBI GATNNNNATC 1 cut(s) 1335
BsaI GGTCTC 2 cut(s) 1530, 1886
BsaJI CCNNGG 3 cut(s) 634, 944, 1975
BsaXI ACNNNNNCTCC 2 cut(s) 261, 291
Bsc4I CCNNNNNNNGG 5 cut(s) 608, 1382, 2114, 2693, 3653
Bse1I ACTGG 5 cut(s) 938, 1278, 1741, 3647, 3663
Bse8I GATNNNNATC 1 cut(s) 1335
BseBI CCWGG 6 cut(s) 412, 755, 945, 1503, 3129, 3516
BseCI ATCGAT 1 cut(s) 2437
BseDI CCNNGG 3 cut(s) 634, 944, 1975
BseJI GATNNNNATC 1 cut(s) 1335
BseLI CCNNNNNNNGG 5 cut(s) 608, 1382, 2114, 2693, 3653
BseMII CTCAG 4 cut(s) 95, 1145, 2186, 2714
BseNI ACTGG 5 cut(s) 938, 1278, 1741, 3647, 3663
BseRI GAGGAG 1 cut(s) 1715
BseXI GCAGC 2 cut(s) 142, 406
BseYI CCCAGC 3 cut(s) 262, 450, 1467
BsgI GTGCAG 2 cut(s) 57, 3180
BshFI GGCC 6 cut(s) 142, 267, 753, 1695, 2202, 3514
BshNI GGYRCC 1 cut(s) 315
BshVI ATCGAT 1 cut(s) 2437
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 3 cut(s) 669, 2027, 2108
BslFI GGGAC 2 cut(s) 961, 1065
BslI CCNNNNNNNGG 5 cut(s) 608, 1382, 2114, 2693, 3653
BsmAI GTCTC 9 cut(s) 1494, 1530, 1653, 1714, 1886, 2030, 2150, 2875, 3615
BsmFI GGGAC 2 cut(s) 961, 1065
BsnI GGCC 6 cut(s) 142, 267, 753, 1695, 2202, 3514
Bso31I GGTCTC 2 cut(s) 1530, 1886
BsoBI CYCGRG 3 cut(s) 669, 2027, 2108
Bsp1286I GDGCHC 1 cut(s) 339
Bsp143I GATC 9 cut(s) 58, 298, 630, 1336, 1406, 1751, 2401, 2456, 3412
Bsp19I CCATGG 1 cut(s) 634
BspACI CCGC 6 cut(s) 43, 313, 568, 643, 850, 1110
BspANI GGCC 6 cut(s) 142, 267, 753, 1695, 2202, 3514
BspCNI CTCAG 4 cut(s) 94, 1144, 2187, 2715
BspDI ATCGAT 1 cut(s) 2437
BspHI TCATGA 1 cut(s) 1528
BspLI GGNNCC 3 cut(s) 300, 317, 632
BspMI ACCTGC 2 cut(s) 719, 3202
BspPI GGATC 5 cut(s) 293, 306, 625, 638, 1344
BspT107I GGYRCC 1 cut(s) 315
BspTNI GGTCTC 2 cut(s) 1530, 1886
BsrI ACTGG 5 cut(s) 938, 1278, 1741, 3647, 3663
BssECI CCNNGG 3 cut(s) 634, 944, 1975
BssMI GATC 9 cut(s) 58, 298, 630, 1336, 1406, 1751, 2401, 2456, 3412
BssNAI GTATAC 3 cut(s) 69, 525, 2918
BssSI CACGAG 2 cut(s) 1151, 3438
BssT1I CCWWGG 2 cut(s) 634, 1975
Bst1107I GTATAC 3 cut(s) 69, 525, 2918
Bst2BI CACGAG 2 cut(s) 1151, 3438
Bst2UI CCWGG 6 cut(s) 412, 755, 945, 1503, 3129, 3516
Bst4CI ACNGT 8 cut(s) 1034, 1437, 1488, 2587, 2611, 3212, 3476, 3526
Bst6I CTCTTC 2 cut(s) 1527, 3337
BstAPI GCANNNNNTGC 3 cut(s) 1450, 3565, 3684
BstC8I GCNNGC 7 cut(s) 641, 1312, 1367, 1909, 2684, 3372, 3689
BstDSI CCRYGG 1 cut(s) 634
BstEII GGTNACC 1 cut(s) 2785
BstKTI GATC 9 cut(s) 61, 301, 633, 1339, 1409, 1754, 2404, 2459, 3415
BstMAI GTCTC 9 cut(s) 1494, 1530, 1653, 1714, 1886, 2030, 2150, 2875, 3615
BstMBI GATC 9 cut(s) 58, 298, 630, 1336, 1406, 1751, 2401, 2456, 3412
BstMWI GCNNNNNNNGC 8 cut(s) 416, 645, 1450, 2848, 3236, 3565, 3675, 3684
BstNI CCWGG 6 cut(s) 412, 755, 945, 1503, 3129, 3516
BstNSI RCATGY 1 cut(s) 643
BstPAI GACNNNNGTC 1 cut(s) 431
BstPI GGTNACC 1 cut(s) 2785
BstSCI CCNGG 6 cut(s) 410, 753, 943, 1501, 3127, 3514
BstSFI CTRYAG 2 cut(s) 1804, 2826
BstV1I GCAGC 2 cut(s) 142, 406
BstV2I GAAGAC 5 cut(s) 277, 1652, 2237, 2813, 3077
BstX2I RGATCY 4 cut(s) 298, 630, 1406, 1751
BstXI CCANNNNNNTGG 1 cut(s) 754
BstYI RGATCY 4 cut(s) 298, 630, 1406, 1751
BstZ17I GTATAC 3 cut(s) 69, 525, 2918
Bsu15I ATCGAT 1 cut(s) 2437
BsuI GTATCC 2 cut(s) 31, 1752
BsuRI GGCC 6 cut(s) 142, 267, 753, 1695, 2202, 3514
BsuTUI ATCGAT 1 cut(s) 2437
BtgI CCRYGG 1 cut(s) 634
BtsI GCAGTG 2 cut(s) 3607, 3676
BtsIMutI CAGTG 5 cut(s) 2616, 2948, 3607, 3670, 3676
BveI ACCTGC 2 cut(s) 719, 3202
Cac8I GCNNGC 7 cut(s) 641, 1312, 1367, 1909, 2684, 3372, 3689
CaiI CAGNNNCTG 1 cut(s) 833
CciI TCATGA 1 cut(s) 1528
Cfr13I GGNCC 4 cut(s) 265, 941, 1681, 3512
ClaI ATCGAT 1 cut(s) 2437
Csp6I GTAC 3 cut(s) 2943, 3248, 3456
CviQI GTAC 3 cut(s) 2943, 3248, 3456
DpnI GATC 9 cut(s) 60, 300, 632, 1338, 1408, 1753, 2403, 2458, 3414
DpnII GATC 9 cut(s) 58, 298, 630, 1336, 1406, 1751, 2401, 2456, 3412
DraI TTTAAA 1 cut(s) 2463
Eam1104I CTCTTC 2 cut(s) 1527, 3337
EarI CTCTTC 2 cut(s) 1527, 3337
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 2 cut(s) 634, 1975
Eco147I AGGCCT 1 cut(s) 142
Eco24I GRGCYC 1 cut(s) 339
Eco31I GGTCTC 2 cut(s) 1530, 1886
Eco32I GATATC 1 cut(s) 470
Eco47I GGWCC 2 cut(s) 941, 1681
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 3 cut(s) 1571, 3089, 3350
Eco88I CYCGRG 3 cut(s) 669, 2027, 2108
Eco91I GGTNACC 1 cut(s) 2785
EcoICRI GAGCTC 1 cut(s) 337
EcoO65I GGTNACC 1 cut(s) 2785
EcoRI GAATTC 2 cut(s) 562, 3287
EcoRII CCWGG 6 cut(s) 410, 753, 943, 1501, 3127, 3514
EcoRV GATATC 1 cut(s) 470
EcoT14I CCWWGG 2 cut(s) 634, 1975
EcoT22I ATGCAT 3 cut(s) 254, 1446, 2971
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 2 cut(s) 634, 1975
FalI AAGNNNNNCTT 2 cut(s) 759, 791
FaqI GGGAC 2 cut(s) 961, 1065
FauI CCCGC 3 cut(s) 50, 575, 636
FbaI TGATCA 1 cut(s) 2401
FblI GTMKAC 3 cut(s) 68, 524, 2917
Fnu4HI GCNGC 3 cut(s) 156, 314, 420
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 3 cut(s) 156, 314, 420
FspBI CTAG 5 cut(s) 77, 171, 951, 1355, 2921
GluI GCNGC 3 cut(s) 156, 314, 420
GsaI CCCAGC 3 cut(s) 266, 454, 1471
GsuI CTGGAG 1 cut(s) 433
HaeIII GGCC 6 cut(s) 142, 267, 753, 1695, 2202, 3514
HincII GTYRAC 4 cut(s) 541, 900, 1540, 1599
HindII GTYRAC 4 cut(s) 541, 900, 1540, 1599
HindIII AAGCTT 3 cut(s) 1991, 2849, 3272
HphI GGTGA 5 cut(s) 88, 2347, 2797, 3407, 3538
HpyAV CCTTC 9 cut(s) 312, 1057, 1321, 1694, 1822, 1934, 2487, 2738, 2912
HpyCH4III ACNGT 8 cut(s) 1034, 1437, 1488, 2587, 2611, 3212, 3476, 3526
HpyCH4IV ACGT 2 cut(s) 2131, 3354
HpyF10VI GCNNNNNNNGC 8 cut(s) 416, 645, 1450, 2848, 3236, 3565, 3675, 3684
HpySE526I ACGT 2 cut(s) 2131, 3354
Ksp22I TGATCA 1 cut(s) 2401
Kzo9I GATC 9 cut(s) 58, 298, 630, 1336, 1406, 1751, 2401, 2456, 3412
LmnI GCTCC 2 cut(s) 830, 2747
Lsp1109I GCAGC 2 cut(s) 142, 406
LweI GCATC 8 cut(s) 994, 1431, 2288, 2429, 2956, 2978, 3032, 3268
MaeI CTAG 5 cut(s) 77, 171, 951, 1355, 2921
MaeII ACGT 2 cut(s) 2131, 3354
MaeIII GTNAC 7 cut(s) 361, 1342, 2577, 2601, 2768, 2785, 3323
MalI GATC 9 cut(s) 60, 300, 632, 1338, 1408, 1753, 2403, 2458, 3414
MboI GATC 9 cut(s) 58, 298, 630, 1336, 1406, 1751, 2401, 2456, 3412
MfeI CAATTG 4 cut(s) 159, 914, 2184, 3013
MflI RGATCY 4 cut(s) 298, 630, 1406, 1751
MhlI GDGCHC 1 cut(s) 339
MlyI GAGTC 4 cut(s) 161, 1716, 2041, 3549
MmeI TCCRAC 2 cut(s) 497, 1449
Mph1103I ATGCAT 3 cut(s) 254, 1446, 2971
MroXI GAANNNNTTC 8 cut(s) 378, 562, 774, 1044, 1476, 1570, 3074, 3111
MslI CAYNNNNRTG 3 cut(s) 557, 1589, 3162
MspR9I CCNGG 6 cut(s) 412, 755, 945, 1503, 3129, 3516
MunI CAATTG 4 cut(s) 159, 914, 2184, 3013
MvaI CCWGG 6 cut(s) 412, 755, 945, 1503, 3129, 3516
MwoI GCNNNNNNNGC 8 cut(s) 416, 645, 1450, 2848, 3236, 3565, 3675, 3684
NcoI CCATGG 1 cut(s) 634
NdeII GATC 9 cut(s) 58, 298, 630, 1336, 1406, 1751, 2401, 2456, 3412
NlaIV GGNNCC 3 cut(s) 300, 317, 632
NmeAIII GCCGAG 1 cut(s) 2289
NmuCI GTSAC 4 cut(s) 1342, 2577, 2601, 2785
NsiI ATGCAT 3 cut(s) 254, 1446, 2971
NspI RCATGY 1 cut(s) 643
PaeI GCATGC 1 cut(s) 643
PaeR7I CTCGAG 2 cut(s) 669, 2027
PagI TCATGA 1 cut(s) 1528
PceI AGGCCT 1 cut(s) 142
PdmI GAANNNNTTC 8 cut(s) 378, 562, 774, 1044, 1476, 1570, 3074, 3111
PflMI CCANNNNNTGG 1 cut(s) 608
PfoI TCCNGGA 1 cut(s) 3127
PkrI GCNGC 3 cut(s) 157, 315, 421
PleI GAGTC 4 cut(s) 161, 1716, 2040, 3548
PpsI GAGTC 4 cut(s) 161, 1716, 2040, 3548
PshAI GACNNNNGTC 1 cut(s) 431
PshBI ATTAAT 1 cut(s) 2072
Psp124BI GAGCTC 1 cut(s) 339
Psp1406I AACGTT 1 cut(s) 2131
Psp6I CCWGG 6 cut(s) 410, 753, 943, 1501, 3127, 3514
PspEI GGTNACC 1 cut(s) 2785
PspFI CCCAGC 3 cut(s) 262, 450, 1467
PspGI CCWGG 6 cut(s) 410, 753, 943, 1501, 3127, 3514
PspN4I GGNNCC 3 cut(s) 300, 317, 632
PspPI GGNCC 4 cut(s) 265, 941, 1681, 3512
PspXI VCTCGAGB 1 cut(s) 669
PstNI CAGNNNCTG 1 cut(s) 833
PsuI RGATCY 4 cut(s) 298, 630, 1406, 1751
RsaI GTAC 3 cut(s) 2944, 3249, 3457
RsaNI GTAC 3 cut(s) 2943, 3248, 3456
RseI CAYNNNNRTG 3 cut(s) 557, 1589, 3162
SacI GAGCTC 1 cut(s) 339
SatI GCNGC 3 cut(s) 156, 314, 420
Sau3AI GATC 9 cut(s) 58, 298, 630, 1336, 1406, 1751, 2401, 2456, 3412
Sau96I GGNCC 4 cut(s) 265, 941, 1681, 3512
SchI GAGTC 4 cut(s) 161, 1716, 2041, 3549
ScrFI CCNGG 6 cut(s) 412, 755, 945, 1503, 3129, 3516
SduI GDGCHC 1 cut(s) 339
SfaNI GCATC 8 cut(s) 994, 1431, 2288, 2429, 2956, 2978, 3032, 3268
SfcI CTRYAG 2 cut(s) 1804, 2826
Sfr274I CTCGAG 2 cut(s) 669, 2027
SinI GGWCC 2 cut(s) 941, 1681
SlaI CTCGAG 2 cut(s) 669, 2027
SmiMI CAYNNNNRTG 3 cut(s) 557, 1589, 3162
SmlI CTYRAG 5 cut(s) 669, 1717, 1747, 2027, 2852
SmoI CTYRAG 5 cut(s) 669, 1717, 1747, 2027, 2852
SphI GCATGC 1 cut(s) 643
SseBI AGGCCT 1 cut(s) 142
SsiI CCGC 6 cut(s) 43, 313, 568, 643, 850, 1110
SspI AATATT 3 cut(s) 1117, 1296, 3139
SspMI CTAG 5 cut(s) 77, 171, 951, 1355, 2921
SstI GAGCTC 1 cut(s) 339
StuI AGGCCT 1 cut(s) 142
StyD4I CCNGG 6 cut(s) 410, 753, 943, 1501, 3127, 3514
StyI CCWWGG 2 cut(s) 634, 1975
TaaI ACNGT 8 cut(s) 1034, 1437, 1488, 2587, 2611, 3212, 3476, 3526
TaiI ACGT 2 cut(s) 2134, 3357
TaqI TCGA 5 cut(s) 670, 1728, 2028, 2437, 3336
TaqII GACCGA 1 cut(s) 720
TatI WGTACW 2 cut(s) 2942, 3247
TauI GCSGC 1 cut(s) 316
TscAI CASTG 5 cut(s) 2616, 2955, 3607, 3670, 3676
TseFI GTSAC 4 cut(s) 1342, 2577, 2601, 2785
TseI GCWGC 2 cut(s) 155, 419
Tsp45I GTSAC 4 cut(s) 1342, 2577, 2601, 2785
TspGWI ACGGA 5 cut(s) 211, 380, 508, 1131, 3533
TspRI CASTG 5 cut(s) 2616, 2955, 3607, 3670, 3676
Van91I CCANNNNNTGG 1 cut(s) 608
VpaK11BI GGWCC 2 cut(s) 941, 1681
VspI ATTAAT 1 cut(s) 2072
XbaI TCTAGA 1 cut(s) 170
XceI RCATGY 1 cut(s) 643
XcmI CCANNNNNNNNNTGG 3 cut(s) 790, 1973, 3164
XhoI CTCGAG 2 cut(s) 669, 2027
XmiI GTMKAC 3 cut(s) 68, 524, 2917
XmnI GAANNNNTTC 8 cut(s) 378, 562, 774, 1044, 1476, 1570, 3074, 3111
XspI CTAG 5 cut(s) 77, 171, 951, 1355, 2921
Zsp2I ATGCAT 3 cut(s) 254, 1446, 2971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.