Prupe.2G059200_v2.0.a1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000105
Physical Location & Seq
Reverse (-)
7379195 .. 7384343
5149 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G059200.1

Sequence Viewer

Length: 3438 bp
ATGGCCGCCCTATTGGCCTCATATAGTTCTTCTTCTTCTTCTTCTCGGGACCCTAAATGGACATACGATGTCTTCCTCAATTTTAGAGGTCAGGACACTCGCAAGAATTTTGTAGACCATTTACATACTGCTTTGGATCAGAAAGGTCTATTCGCATTTAGGGATGTAGAATCTAACTGGGGACAAAGCATCCCGGTAAAAATCTTGGAAGCAATTGAAGAATCAAGATGTGCAGTTGTCATTTTTTCAAGAAATTACGCCGATTCGACATGGTGCTTAGACGAGCTTAGCAAGATTGTAGAATGTAATGAAGGGAGGAGTGGACATATGATACTCCCAGTCTTTTATGATGTGGATCCATCGGACGTACGCAAACAACGAGGGAGTTTCAAGGAGGCTTTCCATAGGCAGGAAGATCGCTTTAGAGACCCAGAGAGGGTGCAAAGGTGGAGAGCTGCACTTACTGTAGTAGCTAATCTCGCCGGATGGAATTTGCGAGAGGGATGTGAACCTGAAGTTATTCAACATATTGTAGAAAGGATTGCCAAACTTAGTCAAACAACCACTAATGTATCCAAGGCTTTAGTTGGAATGGATTCTCGTTTGGAAAAAGTGCTTTCATACTTGGACTTTGGATTGTGTAAAGATGTTCATAGCTTAGGGATATGGGGGATGGGAGGAATAGGCAAGACAACTCTTGCACAAGTGGTGTTTGATACTATACGTGACAAGTTTGAAGCTTGCAGCTTTCTTAACAATGTTCGAGAGGTAACTGAAAAGCAAGGTCTAGTTCGTTTACAAGAAAAACTTCTTTTGGACTTGCTGCAAAGTAATGTAAATATACGGAATACTGATATGGGAAAAAACGTAATAAGGCATAGACTACGTGCTAAAATGGTTCTTATCATTCTTGATGACGTGGATCGGTTAGAACAATTGGAAGCATTGTGTAATCGTAGTTGGTTTGGTTTGGGGAGTAGAATCATCATAACATCACGAGATAAGCATTTGCTGAGAACATTTGGAGTGGACAAAATACATGACGTTGAGGTATTAACTGATGATGAAGCTCTTAAGCTCTTTAGCTGGAAAGCATTCAAGAATTACCAGGTTAAAGAAGAGTATCTCGAACTATCCAAGAATGTTCTAAAATATGCCAATGGCCTTCCATTAGCTCTTGAGATTTTGGGTTCGTTCCTTTGTATGAAAAGTGTACATGAATGGACAATTGTATTGAATAGGCTGATTAAAGAAAGAGATGTTCACAGAAGAATTGTTGATGTGATTAAAATAAGTTTTAATGGGTTAATGGAATCAGATAAGAAAATATTTTTGGACATTGCATGCTTCTTTAAAGGAGAGGACATAGATCGTGTGACAAGAATACTGCAAAGTTGTGGTTACCATCCAAACATTGGTATACCAGTTCTGATTGAAAAAGCTCTAGTAACTGTCTCGAGAGGAAAATTGTGGATGCATGATTTGATACAAGAATTAGGTTGGGATATTGTTCGTCGAGAATGCCGCGACGAGCCTGGCAGACGTAGTCGGTTGTGGCTTCCAGAGGATATCATTCATGTACTCGTAAATAATGAGGTAACAGATGCAGTTGAAGGCATAGCCTTAGACTCGCTTAAATTGGAAGAGGTACGGTGCAACTCAGAGGCCTTCTCCAAGATGCATAATCTTCGGTTTCTGCAAATTCGTAATGTGCATATGACTGAAGGCCCCAAATTTCTTTCTAATGCCTTGGTAGTTCTCGAATGGAGTGAGTACCCTACAAAGTTTCTCCCTCAAAGTTTTCAACCTGTGAAGCTTTGTGAACTTAACTTGTGCTATAGCAGCATTGAACAACTTTGGAATGGAGAAAAGTGCTTGGAGAAGCTGAGATTCATCAATCTTAGCTGGTCCCAGAACCTGACCATGACCCCAGATTTTACAGGTACTCCAAATCTTGAGAGTTTAATTCTTGAAGGTTGTACAAAATTAGCGGTGGTCGACCACTCTATTGCTGGGCTGAAAAGGCTTAAAGTCTTGAATCTTAAAGGATGCAGAAGTCTTGAAAGTCTTCCAAATAAGATTGAAACGAAATGCCTTGAAATTCTTATTCTTTCTAGCTGCTCAAGAATTAAGAAAATTCCAGAATTTGCTGAACCTATGGAACATTTGATGGAGCTTTCTTTAAATGAGACTGCTATAGAAAAATTACCTGCATCAACTGAACTTCTGATTGGCCTTACTTTACTAAATCTAAGAGATTGCTATTATCTCGAGTGTCTTCCACACGACATTAGCAAGTTGAGCTCCCTCAAAAGTCTCAACATTTGTCGCTGCTTTAAACTTAAAGAACTGCCAGAAAGCTTGGGACAGCTTGATTGTTTGGAGGAACTTGATGTGAGCGGTACCTCCTTAAGCACACTACCATCCTCCATTTTCCTTATGAAAAATCTCAAAATTTTATCCCTTCGTGGATGTAAAGGGCTGCCACTTGAGTCAGAAAATACTTCTTGCAACTATCTATCGATGCCCAGAAAGCGGACACGTTTAGTGGGTCTGCAGTTTCCAATTTCTACTTCTGGTTTGTCTTCTTTAACAGAACTGAACCTCAGTGACTGTAATCTTCAGGAGGGAACATTAACGGAAAATCTTGCCTGCCTATTCTCGTTAGTATCTCTAAATCTAAGTAAAAACAATTTTCTGAGTCTTCCCAAAAGCATTAGTCAGCTTTCTAAGCTTCACAGCTTGAATTTGGAAAGTTGTAAGCTTCTTCAGCAAATGCCGGACCTTTCACCAAAAGTGAACTTCGGTGTTGGCTCAGAAGGGAGTAATTCACTGGAAAGGCTGTCATCGTGTTTCAATTATATTAATTGCTCCGAATTGGTTAAGAATCAAGGCTGCAACAATATAGCAATTGCACTGTTAACGAGATTCATTCAGGGAATCCCATATCCAGGACACAGGTTTGAAACTATAATTCCTGGCAGTGAAATTTCTGAGTGGTTCATTGATCGAAGTGCGAGGTCTGGAGTAAGCATGGACCTACCTGAGGACTGGTGTAAGAATAATTGGAGAGGATATGCTTTGTGTGCTGTTTTTGGTCTCGGTCGACGTATCCCTGCCGGTAACCTGCTTGGTAAATGGAAGTATGAGCAAGAGACCTATAGCACTGCACATGGCCTTCGTTGTGAAGTGAAACCCAACAATTTGGGTTCAGGCGGCTGGTGTCCTTTCTTTGGCTGCAGCCCAGAATTAGGCCAAATTGAGTCGGATCACATTTGGCTATCCTTTGTAGGCTGTGAACATTTTGGAACTGCATGGCAAGACAGCTGTCGTCGGCTTGAGTTTTTATTTAAAACTTTAGGCCCTAGCTTGGAGGTGAAGAAGTGTGGAGTCCGTTTGATATATGAGCAAGATATTGGTTTAGGCCCTAATCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1146

Amino Acids

129.86

Weight (kDa)

6.79

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 2218, 3135
Acc65I GGTACC 1 cut(s) 2402
AccB1I GGYRCC 1 cut(s) 2402
AccB7I CCANNNNNTGG 1 cut(s) 1415
AccBSI CCGCTC 1 cut(s) 2400
AccI GTMKAC 4 cut(s) 114, 1420, 1998, 3106
AccII CGCG 1 cut(s) 1527
AciI CCGC 6 cut(s) 6, 1525, 1991, 2400, 2536, 3216
AclWI GGATC 5 cut(s) 144, 350, 363, 930, 3276
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 4 cut(s) 534, 1743, 2606, 2753
AfaI GTAC 8 cut(s) 369, 1215, 1581, 1650, 1775, 1945, 1981, 2404
AfiI CCNNNNNNNGG 9 cut(s) 409, 436, 1415, 2535, 2951, 3046, 3233, 3251, 3370
AflII CTTAAG 2 cut(s) 1073, 2410
AflIII ACRYGT 1 cut(s) 2540
AjiI CACGTC 1 cut(s) 919
AjnI CCWGG 4 cut(s) 1107, 1534, 2950, 2977
AjuI GAANNNNNNNTTGG 6 cut(s) 1316, 1348, 2214, 2246, 2557, 2589
Alw21I GWGCWC 1 cut(s) 2306
Alw26I GTCTC 6 cut(s) 420, 1459, 2183, 2321, 3104, 3149
AlwI GGATC 5 cut(s) 144, 350, 363, 930, 3276
AlwNI CAGNNNCTG 2 cut(s) 1918, 2613
Ama87I CYCGRG 3 cut(s) 45, 1456, 2270
AseI ATTAAT 1 cut(s) 2865
Asp700I GAANNNNTTC 5 cut(s) 519, 595, 807, 1094, 2067
Asp718I GGTACC 1 cut(s) 2402
AspS9I GGNCC 7 cut(s) 49, 1727, 1908, 2782, 3037, 3362, 3425
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 2 cut(s) 2781, 3388
AvaI CYCGRG 3 cut(s) 45, 1456, 2270
AvaII GGWCC 4 cut(s) 49, 1908, 2782, 3037
AxyI CCTNAGG 1 cut(s) 3045
BaeI ACNNNNGTAYC 2 cut(s) 555, 588
BamHI GGATCC 1 cut(s) 355
BanI GGYRCC 1 cut(s) 2402
BanII GRGCYC 1 cut(s) 2306
BauI CACGAG 1 cut(s) 996
BbsI GAAGAC 5 cut(s) 64, 2060, 2270, 2577, 2696
Bbv12I GWGCWC 1 cut(s) 2306
BccI CCATC 6 cut(s) 367, 480, 667, 1413, 2164, 2431
BcgI CGANNNNNNTGC 4 cut(s) 398, 432, 1670, 1704
BciT130I CCWGG 4 cut(s) 1109, 1536, 2952, 2979
BciVI GTATCC 2 cut(s) 583, 3122
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 6 cut(s) 420, 1459, 2183, 2321, 3104, 3149
BfaI CTAG 4 cut(s) 788, 1445, 2115, 3366
BfmI CTRYAG 6 cut(s) 465, 1837, 2196, 2555, 3160, 3238
BfrI CTTAAG 2 cut(s) 1073, 2410
BfuAI ACCTGC 2 cut(s) 2218, 3135
BfuI GTATCC 2 cut(s) 583, 3122
BglI GCCNNNNNGGC 1 cut(s) 14
BlpI GCTNAGC 1 cut(s) 287
Bme1390I CCNGG 5 cut(s) 194, 1109, 1536, 2952, 2979
Bme18I GGWCC 4 cut(s) 49, 1908, 2782, 3037
BmeT110I CYCGRG 3 cut(s) 45, 1456, 2270
BmgBI CACGTC 1 cut(s) 919
BmgT120I GGNCC 7 cut(s) 49, 1727, 1908, 2782, 3037, 3362, 3425
BmiI GGNNCC 6 cut(s) 50, 51, 357, 1729, 1910, 2404
BmrFI CCNGG 5 cut(s) 194, 1109, 1536, 2952, 2979
BmrI ACTGGG 2 cut(s) 187, 332
BmsI GCATC 7 cut(s) 198, 1464, 1594, 1668, 2039, 2222, 2514
BmuI ACTGGG 2 cut(s) 187, 332
BoxI GACNNNNGTC 1 cut(s) 3327
BpiI GAAGAC 5 cut(s) 64, 2060, 2270, 2577, 2696
BplI GAGNNNNNCTC 2 cut(s) 1655, 1687
BpmI CTGGAG 1 cut(s) 3045
Bpu10I CCTNAGC 1 cut(s) 658
Bpu1102I GCTNAGC 1 cut(s) 287
BpuEI CTTGAG 5 cut(s) 1199, 1976, 2107, 2510, 3359
BpuMI CCSGG 1 cut(s) 194
Bsa29I ATCGAT 1 cut(s) 2522
BsaAI YACGTR 2 cut(s) 725, 887
BsaBI GATNNNNATC 1 cut(s) 354
BsaI GGTCTC 3 cut(s) 420, 3104, 3149
BsaJI CCNNGG 2 cut(s) 576, 1749
BsaXI ACNNNNNCTCC 2 cut(s) 1930, 1960
Bsc4I CCNNNNNNNGG 9 cut(s) 409, 436, 1415, 2535, 2951, 3046, 3233, 3251, 3370
Bse118I RCCGGY 1 cut(s) 3119
Bse1I ACTGG 5 cut(s) 182, 338, 1424, 2838, 3056
Bse21I CCTNAGG 1 cut(s) 3045
Bse3DI GCAATG 1 cut(s) 1338
Bse8I GATNNNNATC 1 cut(s) 354
BseBI CCWGG 4 cut(s) 1109, 1536, 2952, 2979
BseCI ATCGAT 1 cut(s) 2522
BseDI CCNNGG 2 cut(s) 576, 1749
BseJI GATNNNNATC 1 cut(s) 354
BseLI CCNNNNNNNGG 9 cut(s) 409, 436, 1415, 2535, 2951, 3046, 3233, 3251, 3370
BseMI GCAATG 1 cut(s) 1338
BseMII CTCAG 8 cut(s) 1004, 1674, 1877, 2620, 2690, 2829, 2985, 3036
BseNI ACTGG 5 cut(s) 182, 338, 1424, 2838, 3056
BseRI GAGGAG 1 cut(s) 331
BseYI CCCAGC 1 cut(s) 2012
BsgI GTGCAG 3 cut(s) 252, 441, 3153
Bsh1236I CGCG 1 cut(s) 1527
Bsh1285I CGRYCG 1 cut(s) 3106
BshNI GGYRCC 1 cut(s) 2402
BshVI ATCGAT 1 cut(s) 2522
BsiEI CGRYCG 1 cut(s) 3106
BsiHKAI GWGCWC 1 cut(s) 2306
BsiHKCI CYCGRG 3 cut(s) 45, 1456, 2270
BsiSI CCGG 4 cut(s) 194, 483, 2780, 3120
BsiWI CGTACG 1 cut(s) 367
BslFI GGGAC 4 cut(s) 62, 195, 1894, 2379
BslI CCNNNNNNNGG 9 cut(s) 409, 436, 1415, 2535, 2951, 3046, 3233, 3251, 3370
BsmAI GTCTC 6 cut(s) 420, 1459, 2183, 2321, 3104, 3149
BsmFI GGGAC 4 cut(s) 62, 195, 1894, 2379
BsmI GAATGC 2 cut(s) 1094, 1526
Bso31I GGTCTC 3 cut(s) 420, 3104, 3149
BsoBI CYCGRG 3 cut(s) 45, 1456, 2270
Bsp1286I GDGCHC 1 cut(s) 2306
Bsp1407I TGTACA 2 cut(s) 1213, 1979
Bsp143I GATC 7 cut(s) 136, 355, 415, 922, 1369, 3007, 3268
Bsp1720I GCTNAGC 1 cut(s) 287
BspACI CCGC 6 cut(s) 6, 1525, 1991, 2400, 2536, 3216
BspCNI CTCAG 8 cut(s) 1005, 1673, 1878, 2619, 2691, 2828, 2986, 3037
BspDI ATCGAT 1 cut(s) 2522
BspFNI CGCG 1 cut(s) 1527
BspLI GGNNCC 6 cut(s) 50, 51, 357, 1729, 1910, 2404
BspMAI CTGCAG 2 cut(s) 2559, 3242
BspMI ACCTGC 2 cut(s) 2218, 3135
BspPI GGATC 5 cut(s) 144, 350, 363, 930, 3276
BspT107I GGYRCC 1 cut(s) 2402
BspTI CTTAAG 2 cut(s) 1073, 2410
BspTNI GGTCTC 3 cut(s) 420, 3104, 3149
BsrBI CCGCTC 1 cut(s) 2400
BsrDI GCAATG 1 cut(s) 1338
BsrFI RCCGGY 1 cut(s) 3119
BsrGI TGTACA 2 cut(s) 1213, 1979
BsrI ACTGG 5 cut(s) 182, 338, 1424, 2838, 3056
BssAI RCCGGY 1 cut(s) 3119
BssECI CCNNGG 2 cut(s) 576, 1749
BssMI GATC 7 cut(s) 136, 355, 415, 922, 1369, 3007, 3268
BssNAI GTATAC 1 cut(s) 1421
BssSI CACGAG 1 cut(s) 996
BssT1I CCWWGG 2 cut(s) 576, 1749
Bst1107I GTATAC 1 cut(s) 1421
Bst2BI CACGAG 1 cut(s) 996
Bst2UI CCWGG 4 cut(s) 1109, 1536, 2952, 2979
Bst4CI ACNGT 5 cut(s) 466, 1453, 1653, 2615, 2919
Bst6I CTCTTC 2 cut(s) 1113, 1638
BstAFI CTTAAG 2 cut(s) 1073, 2410
BstAUI TGTACA 2 cut(s) 1213, 1979
BstBAI YACGTR 2 cut(s) 725, 887
BstC8I GCNNGC 3 cut(s) 742, 1345, 2653
BstEII GGTNACC 2 cut(s) 1400, 3122
BstENI CCTNNNNNAGG 1 cut(s) 3044
BstFNI CGCG 1 cut(s) 1527
BstKTI GATC 7 cut(s) 139, 358, 418, 925, 1372, 3010, 3271
BstMAI GTCTC 6 cut(s) 420, 1459, 2183, 2321, 3104, 3149
BstMBI GATC 7 cut(s) 136, 355, 415, 922, 1369, 3007, 3268
BstMCI CGRYCG 1 cut(s) 3106
BstMWI GCNNNNNNNGC 9 cut(s) 14, 479, 1842, 2023, 2301, 2533, 2731, 2770, 3086
BstNI CCWGG 4 cut(s) 1109, 1536, 2952, 2979
BstNSI RCATGY 1 cut(s) 1347
BstPAI GACNNNNGTC 1 cut(s) 3327
BstPI GGTNACC 2 cut(s) 1400, 3122
BstSCI CCNGG 5 cut(s) 192, 1107, 1534, 2950, 2977
BstSFI CTRYAG 6 cut(s) 465, 1837, 2196, 2555, 3160, 3238
BstUI CGCG 1 cut(s) 1527
BstV2I GAAGAC 5 cut(s) 64, 2060, 2270, 2577, 2696
BstX2I RGATCY 1 cut(s) 355
BstXI CCANNNNNNTGG 1 cut(s) 3205
BstYI RGATCY 1 cut(s) 355
BstZ17I GTATAC 1 cut(s) 1421
Bsu15I ATCGAT 1 cut(s) 2522
Bsu36I CCTNAGG 1 cut(s) 3045
BsuI GTATCC 2 cut(s) 583, 3122
BsuTUI ATCGAT 1 cut(s) 2522
BtrI CACGTC 1 cut(s) 919
BtsI GCAGTG 2 cut(s) 2989, 3165
BtsIMutI CAGTG 5 cut(s) 2614, 2831, 2915, 2989, 3165
BveI ACCTGC 2 cut(s) 2218, 3135
Cac8I GCNNGC 3 cut(s) 742, 1345, 2653
CaiI CAGNNNCTG 2 cut(s) 1918, 2613
Cfr10I RCCGGY 1 cut(s) 3119
Cfr13I GGNCC 7 cut(s) 49, 1727, 1908, 2782, 3037, 3362, 3425
ClaI ATCGAT 1 cut(s) 2522
CsiI ACCWGGT 1 cut(s) 1107
Csp6I GTAC 8 cut(s) 368, 1214, 1580, 1649, 1774, 1944, 1980, 2403
CviQI GTAC 8 cut(s) 368, 1214, 1580, 1649, 1774, 1944, 1980, 2403
DpnI GATC 7 cut(s) 138, 357, 417, 924, 1371, 3009, 3270
DpnII GATC 7 cut(s) 136, 355, 415, 922, 1369, 3007, 3268
DraI TTTAAA 4 cut(s) 1354, 2184, 2338, 3352
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 1113, 1638
EarI CTCTTC 2 cut(s) 1113, 1638
Ecl136II GAGCTC 1 cut(s) 2304
Eco130I CCWWGG 2 cut(s) 576, 1749
Eco147I AGGCCT 1 cut(s) 1667
Eco24I GRGCYC 1 cut(s) 2306
Eco31I GGTCTC 3 cut(s) 420, 3104, 3149
Eco32I GATATC 1 cut(s) 1570
Eco47I GGWCC 4 cut(s) 49, 1908, 2782, 3037
Eco53kI GAGCTC 1 cut(s) 2304
Eco57I CTGAAG 4 cut(s) 534, 1743, 2606, 2753
Eco81I CCTNAGG 1 cut(s) 3045
Eco88I CYCGRG 3 cut(s) 45, 1456, 2270
Eco91I GGTNACC 2 cut(s) 1400, 3122
EcoICRI GAGCTC 1 cut(s) 2304
EcoNI CCTNNNNNAGG 1 cut(s) 3044
EcoO109I RGGNCCY 4 cut(s) 49, 1727, 3362, 3425
EcoO65I GGTNACC 2 cut(s) 1400, 3122
EcoRII CCWGG 4 cut(s) 1107, 1534, 2950, 2977
EcoRV GATATC 1 cut(s) 1570
EcoT14I CCWWGG 2 cut(s) 576, 1749
EcoT22I ATGCAT 2 cut(s) 1479, 1683
EcoT38I GRGCYC 1 cut(s) 2306
ErhI CCWWGG 2 cut(s) 576, 1749
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 4 cut(s) 62, 195, 1894, 2379
FauNDI CATATG 2 cut(s) 327, 1716
FblI GTMKAC 4 cut(s) 114, 1420, 1998, 3106
FriOI GRGCYC 1 cut(s) 2306
FspBI CTAG 4 cut(s) 788, 1445, 2115, 3366
GsaI CCCAGC 1 cut(s) 2016
GsuI CTGGAG 1 cut(s) 3045
HapII CCGG 4 cut(s) 194, 483, 2780, 3120
HincII GTYRAC 3 cut(s) 1999, 2922, 3107
HindII GTYRAC 3 cut(s) 1999, 2922, 3107
HindIII AAGCTT 5 cut(s) 738, 1814, 2359, 2732, 2762
HpaI GTTAAC 1 cut(s) 2922
HpaII CCGG 4 cut(s) 194, 483, 2780, 3120
HphI GGTGA 2 cut(s) 2781, 3388
Hpy99I CGWCG 4 cut(s) 1518, 1532, 3111, 3336
HpyAV CCTTC 9 cut(s) 305, 1175, 1607, 1678, 1718, 1967, 2474, 2813, 3188
HpyCH4III ACNGT 5 cut(s) 466, 1453, 1653, 2615, 2919
HpyCH4IV ACGT 9 cut(s) 366, 724, 867, 886, 918, 1044, 1543, 2542, 3109
HpyF10VI GCNNNNNNNGC 9 cut(s) 14, 479, 1842, 2023, 2301, 2533, 2731, 2770, 3086
HpySE526I ACGT 9 cut(s) 366, 724, 867, 886, 918, 1044, 1543, 2542, 3109
KflI GGGWCCC 1 cut(s) 49
KpnI GGTACC 1 cut(s) 2406
KspAI GTTAAC 1 cut(s) 2922
Kzo9I GATC 7 cut(s) 136, 355, 415, 922, 1369, 3007, 3268
LmnI GCTCC 3 cut(s) 2173, 2309, 2876
LweI GCATC 7 cut(s) 198, 1464, 1594, 1668, 2039, 2222, 2514
MabI ACCWGGT 1 cut(s) 1107
MaeI CTAG 4 cut(s) 788, 1445, 2115, 3366
MaeII ACGT 9 cut(s) 366, 724, 867, 886, 918, 1044, 1543, 2542, 3109
MaeIII GTNAC 8 cut(s) 725, 769, 1375, 1400, 1447, 1597, 2609, 3122
MalI GATC 7 cut(s) 138, 357, 417, 924, 1371, 3009, 3270
MbiI CCGCTC 1 cut(s) 2400
MboI GATC 7 cut(s) 136, 355, 415, 922, 1369, 3007, 3268
MfeI CAATTG 4 cut(s) 213, 935, 1227, 2910
MflI RGATCY 1 cut(s) 355
MhlI GDGCHC 1 cut(s) 2306
MlyI GAGTC 5 cut(s) 1622, 2501, 2710, 3272, 3399
MmeI TCCRAC 2 cut(s) 568, 3246
Mph1103I ATGCAT 2 cut(s) 1479, 1683
MroXI GAANNNNTTC 5 cut(s) 519, 595, 807, 1094, 2067
MspA1I CMGCKG 1 cut(s) 3327
MspCI CTTAAG 2 cut(s) 1073, 2410
MspI CCGG 4 cut(s) 194, 483, 2780, 3120
MspR9I CCNGG 5 cut(s) 194, 1109, 1536, 2952, 2979
MunI CAATTG 4 cut(s) 213, 935, 1227, 2910
Mva1269I GAATGC 2 cut(s) 1094, 1526
MvaI CCWGG 4 cut(s) 1109, 1536, 2952, 2979
MvnI CGCG 1 cut(s) 1527
MwoI GCNNNNNNNGC 9 cut(s) 14, 479, 1842, 2023, 2301, 2533, 2731, 2770, 3086
NciI CCSGG 1 cut(s) 194
NdeI CATATG 2 cut(s) 327, 1716
NdeII GATC 7 cut(s) 136, 355, 415, 922, 1369, 3007, 3268
NlaIV GGNNCC 6 cut(s) 50, 51, 357, 1729, 1910, 2404
NmuCI GTSAC 3 cut(s) 725, 1375, 2609
NsiI ATGCAT 2 cut(s) 1479, 1683
NspI RCATGY 1 cut(s) 1347
PaeI GCATGC 1 cut(s) 1347
PaeR7I CTCGAG 2 cut(s) 1456, 2270
PceI AGGCCT 1 cut(s) 1667
PcsI WCGNNNNNNNCGW 1 cut(s) 376
PctI GAATGC 2 cut(s) 1094, 1526
PdmI GAANNNNTTC 5 cut(s) 519, 595, 807, 1094, 2067
Pfl23II CGTACG 1 cut(s) 367
PflFI GACNNNGTC 1 cut(s) 1545
PflMI CCANNNNNTGG 1 cut(s) 1415
PfoI TCCNGGA 1 cut(s) 2950
PleI GAGTC 5 cut(s) 1622, 2500, 2709, 3271, 3398
PpsI GAGTC 5 cut(s) 1622, 2500, 2709, 3271, 3398
Ppu21I YACGTR 2 cut(s) 725, 887
PpuMI RGGWCCY 1 cut(s) 49
PshAI GACNNNNGTC 1 cut(s) 3327
PshBI ATTAAT 1 cut(s) 2865
Psp124BI GAGCTC 1 cut(s) 2306
Psp5II RGGWCCY 1 cut(s) 49
Psp6I CCWGG 4 cut(s) 1107, 1534, 2950, 2977
PspEI GGTNACC 2 cut(s) 1400, 3122
PspFI CCCAGC 1 cut(s) 2012
PspGI CCWGG 4 cut(s) 1107, 1534, 2950, 2977
PspLI CGTACG 1 cut(s) 367
PspN4I GGNNCC 6 cut(s) 50, 51, 357, 1729, 1910, 2404
PspPI GGNCC 7 cut(s) 49, 1727, 1908, 2782, 3037, 3362, 3425
PspPPI RGGWCCY 1 cut(s) 49
PstI CTGCAG 2 cut(s) 2559, 3242
PstNI CAGNNNCTG 2 cut(s) 1918, 2613
PsuI RGATCY 1 cut(s) 355
PsyI GACNNNGTC 1 cut(s) 1545
PvuII CAGCTG 1 cut(s) 3327
RsaI GTAC 8 cut(s) 369, 1215, 1581, 1650, 1775, 1945, 1981, 2404
RsaNI GTAC 8 cut(s) 368, 1214, 1580, 1649, 1774, 1944, 1980, 2403
SacI GAGCTC 1 cut(s) 2306
SalI GTCGAC 2 cut(s) 1997, 3105
Sau3AI GATC 7 cut(s) 136, 355, 415, 922, 1369, 3007, 3268
Sau96I GGNCC 7 cut(s) 49, 1727, 1908, 2782, 3037, 3362, 3425
SchI GAGTC 5 cut(s) 1622, 2501, 2710, 3272, 3399
ScrFI CCNGG 5 cut(s) 194, 1109, 1536, 2952, 2979
SduI GDGCHC 1 cut(s) 2306
SexAI ACCWGGT 1 cut(s) 1107
SfaNI GCATC 7 cut(s) 198, 1464, 1594, 1668, 2039, 2222, 2514
SfcI CTRYAG 6 cut(s) 465, 1837, 2196, 2555, 3160, 3238
Sfr274I CTCGAG 2 cut(s) 1456, 2270
SinI GGWCC 4 cut(s) 49, 1908, 2782, 3037
SlaI CTCGAG 2 cut(s) 1456, 2270
SmlI CTYRAG 9 cut(s) 1073, 1178, 1456, 1955, 2122, 2270, 2410, 2489, 3338
SmoI CTYRAG 9 cut(s) 1073, 1178, 1456, 1955, 2122, 2270, 2410, 2489, 3338
SphI GCATGC 1 cut(s) 1347
SseBI AGGCCT 1 cut(s) 1667
SsiI CCGC 6 cut(s) 6, 1525, 1991, 2400, 2536, 3216
SspI AATATT 1 cut(s) 1329
SspMI CTAG 4 cut(s) 788, 1445, 2115, 3366
SstI GAGCTC 1 cut(s) 2306
StuI AGGCCT 1 cut(s) 1667
StyD4I CCNGG 5 cut(s) 192, 1107, 1534, 2950, 2977
StyI CCWWGG 2 cut(s) 576, 1749
TaaI ACNGT 5 cut(s) 466, 1453, 1653, 2615, 2919
TaiI ACGT 9 cut(s) 369, 727, 870, 889, 921, 1047, 1546, 2545, 3112
TaqII GACCGA 1 cut(s) 3092
TatI WGTACW 3 cut(s) 1213, 1579, 1979
TauI GCSGC 3 cut(s) 8, 1527, 3219
TscAI CASTG 5 cut(s) 2614, 2838, 2922, 2989, 3172
TseFI GTSAC 3 cut(s) 725, 1375, 2609
Tsp45I GTSAC 3 cut(s) 725, 1375, 2609
TspGWI ACGGA 3 cut(s) 859, 2654, 3383
TspRI CASTG 5 cut(s) 2614, 2838, 2922, 2989, 3172
Tth111I GACNNNGTC 1 cut(s) 1545
Van91I CCANNNNNTGG 1 cut(s) 1415
Vha464I CTTAAG 2 cut(s) 1073, 2410
VpaK11BI GGWCC 4 cut(s) 49, 1908, 2782, 3037
VspI ATTAAT 1 cut(s) 2865
XagI CCTNNNNNAGG 1 cut(s) 3044
XceI RCATGY 1 cut(s) 1347
XcmI CCANNNNNNNNNTGG 2 cut(s) 1412, 2009
XhoI CTCGAG 2 cut(s) 1456, 2270
XmiI GTMKAC 4 cut(s) 114, 1420, 1998, 3106
XmnI GAANNNNTTC 5 cut(s) 519, 595, 807, 1094, 2067
XspI CTAG 4 cut(s) 788, 1445, 2115, 3366
Zsp2I ATGCAT 2 cut(s) 1479, 1683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.