RchiOBHm_Chr1g0323221

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000105
Physical Location & Seq
Reverse (-)
10773383 .. 10779110
5728 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55314

Sequence Viewer

Length: 3567 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTGTTTCTAAATTTCAGGGGGGCAGATGCCCGCCATCCTTTTACCAGTAATCTGTATGCTGCACTAAATCAGAAAGGAATTCTCACCTTCATGGATGACGCAGAGCTTGAGAAAGGAAAATCCATTAGACCTGAACTTTCAGCTGCAATTGAGGAGTCTAGATCCGCGATTGTCATTCTCTCACGGAACTATGCTCGTTCGTCATGGTGCTTGAATGAACTCGTCAAGATTATTCAATGCATGAAAGAGAAGGGTCAACAAGTCCTCCCCGTCTTCTACAGGGTGGATCCTTCTGAAGTGCGGCACCAAAGTGGAAGATTTGAGCTCAAAAGGAAATCCCAAGTGGATGTGGAAGTAAGGGAACATGAAGAAGTTTATCGGAAGAATCAGGACAGACAAGATGCCTGGAGAGCTGCTTTGACTGAGGTCGCCAATCTTTCTGGCTGGCATACCAAGAATTATGGAGAAGATTCAAGAATATTTGACGAAATTGTTAATCATATACTAAAAAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGGTTCCCGCACTGATGATTTATTAAGCAATTACATATTTCCGCAGCTTTGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGGGCTATCCACGATGATATTTGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCTTAGTTTCTCTACAAGAAAAGCTTCTTTCCAGAATCATGATGGCAAAAGTTGAAAATATAGAGGATGAATTCACAGGAGCTGCAATGATAGAAAGGAGGTCGTGTAGAATAAAGGTGCTTCTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAGGATTATCATAACCACCACAGATATTCAATTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCCAACGGGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAACATCCCCCACCAGAAGATTATTTGGATCTGTGCTACGATATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTCTCTGGTAGAGGGGCTGATAAATGGGCAAGTGCAATAGATAGGCTGAGGAGCACACCAGATAAACGCATTATTGACGTGCTTCGGATTAGTTTTGATGACCTGCATAAAAAAGAGAAAGAAATATTCCTTCATATTGCTTGCTTTTACAAGGGGATGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTTCAACCTGTCATTGGTTTAGATGTTCTTGCTGATAGATCTCTCATAACTGTCTCCAACAATGATTTGTCGATGCATGATTTGCTACAAGAAATGGGCCGGGAAATTGTTCGTGAACAGTCTCCTGATGAGCCAGGCAAATGTAGTAGATTATGGTCTCATAAAGACATCAACAAGGTGCTGGAGAGAAATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTGAATTACAAGTGGCCCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTATTCTTCTCCATATTCGTAATGTTGACCTTCCGGATGGCCTTACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTAAGATCTCTTCCACAAAATTTTGAAGCAGATGAACTTATTGAACTTAACTTCTGCCGCAGCAACATTAAACAGCTTTGGAAGGGGACAAAGAATTTTGACAAGTTGAAATTAATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCACAGGTGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTAAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCATGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTCAGTGCTTTGTCTTGATGAGACTGCCATTGAGGAATTGCCTGTTTCAATTGAACGGCTGATTGGCCTTGTGTCATTGAATCTAAGCAACTGCAGAAATCTTGTATGTCTTCCAAGCACCATCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAATATCAGGTAAATGTAGGGGAGATGGACTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATTGAAATGTCATCTACCCTTGGTCTCATAAAATATGAGAGAGGTTCAAACTTTCATGGATGCGAAGTAGTTTTGGAATCTTTAAATGAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGAGCCAATGAGTTTCCGCTTGCCTATATCTGGTCTCTGTAATTTAACATATCTGAATCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAATGAATTTGGTTACTTTCCCTCTTTAGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGCATTCCTTCAGGCATTCGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGACTAAAGAGATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATGAAGGATGCAATACCATAGCATTTGAAATGCTGAGGACACTCATGTATCAGGGAATCTCTAATAAGAGGGAAACCTTTCAAATTGTAATTCCTGGAAGTAATATCCATGAGTGGTTCAGCCATCAGAGTGTTGGGTGTTCCTTATGTGTATCTCTACCTGCACATTGGAATAAGAGTCGGTTTTTGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATGAGCACCATCCGGTGGATGAGCTTTATGTAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCAATGGAAGAGAATTGGAAGTGTATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTGAGTCAGATCACCTGTGGCTATTCTATGTATCTTGTGATAAATACTTTGGTATAGAGAGTTGGCTTTGCGGTTTGAATCATCTTGAGTTCTTATTTGAAACTAGAGGGCCGGGTCTGAAGGTGAAACAGTGTGGAATCCGTCTGATATATGAGCAAGATGTGCAAGAGTCGAACAAAACAATGCAAGAGTCGAACGAAACAACCACTCAATCAAGCAGTAGGATGTCTCTTAAGAGTATATATGATTGGTTTCAACGTTTCAGCTACATTGGAAACTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1188

Amino Acids

135.29

Weight (kDa)

6.12

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 191 1.4e-52 TIR domain
TIR_2 PF13676 14 - 110 1e-15 TIR domain
NB-ARC PF00931 216 - 371 1.7e-17 NB-ARC domain
NACHT PF05729 221 - 317 2.4e-07 NACHT domain
WHD_ROQ1 PF23282 439 - 509 3e-23 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 677 - 777 5.5e-09 Leucine-rich repeat region
LRR_13 PF23286 698 - 783 3.9e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 1001 - 1150 2.4e-16 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 755, 1317, 3079
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 2 cut(s) 1621, 3145
AccI GTMKAC 2 cut(s) 560, 2794
AccII CGCG 1 cut(s) 206
AccIII TCCGGA 1 cut(s) 1723
AciI CCGC 9 cut(s) 70, 204, 340, 604, 638, 679, 1840, 2557, 3354
AclI AACGTT 2 cut(s) 2167, 3541
AclWI GGATC 8 cut(s) 195, 320, 333, 661, 674, 936, 1146, 1380
AcuI CTGAAG 4 cut(s) 354, 2688, 3230, 3422
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 2821, 3126
AfiI CCNNNNNNNGG 4 cut(s) 1418, 1621, 2570, 3145
AflII CTTAAG 2 cut(s) 1783, 3515
AjiI CACGTC 1 cut(s) 1285
AjnI CCWGG 5 cut(s) 443, 979, 1537, 3004, 3279
AjuI GAANNNNNNNTTGG 4 cut(s) 2217, 2249, 2726, 2758
AleI CACNNNNGTG 1 cut(s) 348
Alw21I GWGCWC 3 cut(s) 366, 1262, 3138
AlwI GGATC 8 cut(s) 195, 320, 333, 661, 674, 936, 1146, 1380
AlwNI CAGNNNCTG 1 cut(s) 869
Ama87I CYCGRG 3 cut(s) 705, 2063, 2144
Aor13HI TCCGGA 1 cut(s) 1723
AoxI GGCC 6 cut(s) 1053, 1501, 1650, 1729, 2236, 3392
ApeKI GCWGC 6 cut(s) 98, 182, 452, 640, 869, 1842
AseI ATTAAT 2 cut(s) 1895, 2108
Asp700I GAANNNNTTC 6 cut(s) 598, 810, 1080, 1512, 1606, 2988
AspS9I GGNCC 4 cut(s) 977, 1501, 1651, 3392
AsuC2I CCSGG 2 cut(s) 1505, 3396
AsuHPI GGTGA 4 cut(s) 115, 2630, 3287, 3418
AvaI CYCGRG 3 cut(s) 705, 2063, 2144
AvaII GGWCC 1 cut(s) 977
BaeI ACNNNNGTAYC 2 cut(s) 2942, 2975
BamHI GGATCC 2 cut(s) 325, 666
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 1187
BbsI GAAGAC 2 cut(s) 304, 2273
Bbv12I GWGCWC 3 cut(s) 366, 1262, 3138
BbvCI CCTCAGC 2 cut(s) 1253, 2945
BbvI GCAGC 6 cut(s) 85, 169, 439, 652, 856, 1854
BceAI ACGGC 1 cut(s) 2243
BcgI CGANNNNNNTGC 2 cut(s) 2044, 2078
BciT130I CCWGG 5 cut(s) 445, 981, 1539, 3006, 3281
BciVI GTATCC 1 cut(s) 1788
BcnI CCSGG 2 cut(s) 1505, 3396
BfaI CTAG 4 cut(s) 198, 1391, 2798, 3387
BfmI CTRYAG 2 cut(s) 316, 2263
BfrI CTTAAG 2 cut(s) 1783, 3515
BfuAI ACCTGC 3 cut(s) 755, 1317, 3079
BfuI GTATCC 1 cut(s) 1788
BglII AGATCT 3 cut(s) 1442, 1787, 1969
BisI GCNGC 8 cut(s) 99, 183, 341, 453, 641, 870, 1840, 1843
BlsI GCNGC 8 cut(s) 100, 184, 342, 454, 642, 871, 1841, 1844
Bme1390I CCNGG 7 cut(s) 445, 981, 1505, 1539, 3006, 3281, 3396
Bme18I GGWCC 1 cut(s) 977
BmeT110I CYCGRG 3 cut(s) 705, 2063, 2144
BmgBI CACGTC 1 cut(s) 1285
BmgT120I GGNCC 4 cut(s) 977, 1501, 1651, 3392
BmiI GGNNCC 4 cut(s) 327, 344, 601, 668
BmrFI CCNGG 7 cut(s) 445, 981, 1505, 1539, 3006, 3281, 3396
BmrI ACTGGG 1 cut(s) 1782
BmsI GCATC 9 cut(s) 55, 430, 1030, 1467, 2324, 2465, 2833, 2855, 2909
BmuI ACTGGG 1 cut(s) 1782
BoxI GACNNNNGTC 1 cut(s) 464
BpiI GAAGAC 2 cut(s) 304, 2273
BpmI CTGGAG 2 cut(s) 466, 1607
Bpu10I CCTNAGC 3 cut(s) 1167, 1253, 2945
BpuEI CTTGAG 5 cut(s) 167, 1774, 1979, 2750, 3389
BpuMI CCSGG 2 cut(s) 1505, 3396
BsaBI GATNNNNATC 2 cut(s) 927, 1371
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 4 cut(s) 1566, 1922, 2444, 2579
BsaJI CCNNGG 5 cut(s) 670, 980, 1615, 2011, 2434
BsaWI WCCGGW 2 cut(s) 1723, 3142
BsaXI ACNNNNNCTCC 4 cut(s) 288, 318, 877, 907
Bsc4I CCNNNNNNNGG 4 cut(s) 1418, 1621, 2570, 3145
Bse1I ACTGG 4 cut(s) 84, 974, 1777, 3566
Bse3DI GCAATG 2 cut(s) 879, 2917
Bse8I GATNNNNATC 2 cut(s) 927, 1371
BseAI TCCGGA 1 cut(s) 1723
BseBI CCWGG 5 cut(s) 445, 981, 1539, 3006, 3281
BseDI CCNNGG 5 cut(s) 670, 980, 1615, 2011, 2434
BseJI GATNNNNATC 2 cut(s) 927, 1371
BseLI CCNNNNNNNGG 4 cut(s) 1418, 1621, 2570, 3145
BseMI GCAATG 2 cut(s) 879, 2917
BseMII CTCAG 5 cut(s) 453, 1181, 1244, 2591, 2936
BseNI ACTGG 4 cut(s) 84, 974, 1777, 3566
BseRI GAGGAG 3 cut(s) 206, 1270, 1751
BseXI GCAGC 6 cut(s) 85, 169, 439, 652, 856, 1854
BsgI GTGCAG 2 cut(s) 84, 3057
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 6 cut(s) 1055, 1503, 1652, 1731, 2238, 3394
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 3 cut(s) 366, 1262, 3138
BsiHKCI CYCGRG 3 cut(s) 705, 2063, 2144
BsiSI CCGG 4 cut(s) 1504, 1724, 3143, 3395
BslFI GGGAC 1 cut(s) 1882
BslI CCNNNNNNNGG 4 cut(s) 1418, 1621, 2570, 3145
BsmFI GGGAC 1 cut(s) 1882
BsmI GAATGC 2 cut(s) 2697, 2709
BsnI GGCC 6 cut(s) 1055, 1503, 1652, 1731, 2238, 3394
Bso31I GGTCTC 4 cut(s) 1566, 1922, 2444, 2579
BsoBI CYCGRG 3 cut(s) 705, 2063, 2144
Bsp1286I GDGCHC 3 cut(s) 366, 1262, 3138
Bsp13I TCCGGA 1 cut(s) 1723
Bsp19I CCATGG 1 cut(s) 670
BspACI CCGC 9 cut(s) 70, 204, 340, 604, 638, 679, 1840, 2557, 3354
BspANI GGCC 6 cut(s) 1055, 1503, 1652, 1731, 2238, 3394
BspCNI CTCAG 5 cut(s) 454, 1180, 1245, 2592, 2937
BspEI TCCGGA 1 cut(s) 1723
BspFNI CGCG 1 cut(s) 206
BspHI TCATGA 1 cut(s) 825
BspLI GGNNCC 4 cut(s) 327, 344, 601, 668
BspMAI CTGCAG 1 cut(s) 2267
BspMI ACCTGC 3 cut(s) 755, 1317, 3079
BspPI GGATC 8 cut(s) 195, 320, 333, 661, 674, 936, 1146, 1380
BspT107I GGYRCC 1 cut(s) 342
BspTI CTTAAG 2 cut(s) 1783, 3515
BspTNI GGTCTC 4 cut(s) 1566, 1922, 2444, 2579
BsrDI GCAATG 2 cut(s) 879, 2917
BsrI ACTGG 4 cut(s) 84, 974, 1777, 3566
BssECI CCNNGG 5 cut(s) 670, 980, 1615, 2011, 2434
BssNAI GTATAC 1 cut(s) 561
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1187
BssT1I CCWWGG 4 cut(s) 670, 1615, 2011, 2434
Bst1107I GTATAC 1 cut(s) 561
Bst2BI CACGAG 1 cut(s) 1187
Bst2UI CCWGG 5 cut(s) 445, 981, 1539, 3006, 3281
Bst4CI ACNGT 5 cut(s) 1070, 1456, 1524, 2381, 3414
Bst6I CTCTTC 3 cut(s) 19, 1797, 3217
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 2 cut(s) 1783, 3515
BstAPI GCANNNNNTGC 2 cut(s) 1486, 3445
BstC8I GCNNGC 7 cut(s) 70, 485, 677, 1348, 1403, 2561, 3252
BstDEI CTNAG 9 cut(s) 462, 790, 1167, 1253, 2255, 2600, 2675, 2724, 2945
BstDSI CCRYGG 1 cut(s) 670
BstFNI CGCG 1 cut(s) 206
BstMWI GCNNNNNNNGC 6 cut(s) 449, 681, 1486, 2725, 3113, 3445
BstNI CCWGG 5 cut(s) 445, 981, 1539, 3006, 3281
BstNSI RCATGY 1 cut(s) 679
BstPAI GACNNNNGTC 1 cut(s) 464
BstSCI CCNGG 7 cut(s) 443, 979, 1503, 1537, 3004, 3279, 3394
BstSFI CTRYAG 2 cut(s) 316, 2263
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 6 cut(s) 85, 169, 439, 652, 856, 1854
BstV2I GAAGAC 2 cut(s) 304, 2273
BstX2I RGATCY 7 cut(s) 200, 325, 666, 1138, 1442, 1787, 1969
BstYI RGATCY 7 cut(s) 200, 325, 666, 1138, 1442, 1787, 1969
BstZ17I GTATAC 1 cut(s) 561
BsuI GTATCC 1 cut(s) 1788
BsuRI GGCC 6 cut(s) 1055, 1503, 1652, 1731, 2238, 3394
BtgI CCRYGG 1 cut(s) 670
BtrI CACGTC 1 cut(s) 1285
BtsIMutI CAGTG 4 cut(s) 606, 2181, 2825, 3419
BveI ACCTGC 3 cut(s) 755, 1317, 3079
Cac8I GCNNGC 7 cut(s) 70, 485, 677, 1348, 1403, 2561, 3252
CaiI CAGNNNCTG 1 cut(s) 869
CciI TCATGA 1 cut(s) 825
Cfr13I GGNCC 4 cut(s) 977, 1501, 1651, 3392
CseI GACGC 1 cut(s) 146
Csp6I GTAC 2 cut(s) 2820, 3125
CviQI GTAC 2 cut(s) 2820, 3125
DdeI CTNAG 9 cut(s) 462, 790, 1167, 1253, 2255, 2600, 2675, 2724, 2945
DraI TTTAAA 1 cut(s) 2499
Eam1104I CTCTTC 3 cut(s) 19, 1797, 3217
EarI CTCTTC 3 cut(s) 19, 1797, 3217
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 4 cut(s) 670, 1615, 2011, 2434
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 4 cut(s) 1566, 1922, 2444, 2579
Eco47I GGWCC 1 cut(s) 977
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 4 cut(s) 354, 2688, 3230, 3422
Eco88I CYCGRG 3 cut(s) 705, 2063, 2144
EcoICRI GAGCTC 1 cut(s) 364
EcoRI GAATTC 3 cut(s) 117, 857, 3167
EcoRII CCWGG 5 cut(s) 443, 979, 1537, 3004, 3279
EcoT14I CCWWGG 4 cut(s) 670, 1615, 2011, 2434
EcoT22I ATGCAT 3 cut(s) 281, 1482, 2848
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 4 cut(s) 670, 1615, 2011, 2434
FalI AAGNNNNNCTT 2 cut(s) 795, 827
FaqI GGGAC 1 cut(s) 1882
FauI CCCGC 3 cut(s) 77, 611, 672
FblI GTMKAC 2 cut(s) 560, 2794
Fnu4HI GCNGC 8 cut(s) 99, 183, 341, 453, 641, 870, 1840, 1843
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 8 cut(s) 99, 183, 341, 453, 641, 870, 1840, 1843
FspBI CTAG 4 cut(s) 198, 1391, 2798, 3387
GluI GCNGC 8 cut(s) 99, 183, 341, 453, 641, 870, 1840, 1843
GsuI CTGGAG 2 cut(s) 466, 1607
HaeIII GGCC 6 cut(s) 1055, 1503, 1652, 1731, 2238, 3394
HapII CCGG 4 cut(s) 1504, 1724, 3143, 3395
HgaI GACGC 1 cut(s) 146
Hin1I GRCGYC 1 cut(s) 5
HincII GTYRAC 6 cut(s) 9, 296, 577, 936, 1635, 1717
HindII GTYRAC 6 cut(s) 9, 296, 577, 936, 1635, 1717
HindIII AAGCTT 3 cut(s) 809, 2027, 2726
HpaII CCGG 4 cut(s) 1504, 1724, 3143, 3395
HphI GGTGA 4 cut(s) 115, 2630, 3287, 3418
Hpy166II GTNNAC 9 cut(s) 9, 296, 561, 577, 936, 1520, 1635, 1717, 2795
Hpy8I GTNNAC 9 cut(s) 9, 296, 561, 577, 936, 1520, 1635, 1717, 2795
Hpy99I CGWCG 1 cut(s) 24
HpyCH4III ACNGT 5 cut(s) 1070, 1456, 1524, 2381, 3414
HpyCH4IV ACGT 3 cut(s) 1284, 2167, 3541
HpyF10VI GCNNNNNNNGC 6 cut(s) 449, 681, 1486, 2725, 3113, 3445
HpyF3I CTNAG 9 cut(s) 462, 790, 1167, 1253, 2255, 2600, 2675, 2724, 2945
HpySE526I ACGT 3 cut(s) 1284, 2167, 3541
Hsp92I GRCGYC 1 cut(s) 5
Kpn2I TCCGGA 1 cut(s) 1723
LmnI GCTCC 3 cut(s) 866, 1257, 2624
Lsp1109I GCAGC 6 cut(s) 85, 169, 439, 652, 856, 1854
LweI GCATC 9 cut(s) 55, 430, 1030, 1467, 2324, 2465, 2833, 2855, 2909
MaeI CTAG 4 cut(s) 198, 1391, 2798, 3387
MaeII ACGT 3 cut(s) 1284, 2167, 3541
MaeIII GTNAC 3 cut(s) 1378, 2645, 2662
MfeI CAATTG 5 cut(s) 186, 1016, 2220, 2415, 2890
MflI RGATCY 7 cut(s) 200, 325, 666, 1138, 1442, 1787, 1969
MhlI GDGCHC 3 cut(s) 366, 1262, 3138
MlyI GAGTC 7 cut(s) 203, 1752, 2077, 3097, 3296, 3461, 3482
MmeI TCCRAC 2 cut(s) 1485, 2134
Mph1103I ATGCAT 3 cut(s) 281, 1482, 2848
MroI TCCGGA 1 cut(s) 1723
MroXI GAANNNNTTC 6 cut(s) 598, 810, 1080, 1512, 1606, 2988
MslI CAYNNNNRTG 5 cut(s) 128, 348, 593, 1625, 3039
MspA1I CMGCKG 1 cut(s) 182
MspCI CTTAAG 2 cut(s) 1783, 3515
MspI CCGG 4 cut(s) 1504, 1724, 3143, 3395
MspR9I CCNGG 7 cut(s) 445, 981, 1505, 1539, 3006, 3281, 3396
MunI CAATTG 5 cut(s) 186, 1016, 2220, 2415, 2890
Mva1269I GAATGC 2 cut(s) 2697, 2709
MvaI CCWGG 5 cut(s) 445, 981, 1539, 3006, 3281
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 6 cut(s) 449, 681, 1486, 2725, 3113, 3445
NciI CCSGG 2 cut(s) 1505, 3396
NcoI CCATGG 1 cut(s) 670
NlaIV GGNNCC 4 cut(s) 327, 344, 601, 668
NmeAIII GCCGAG 1 cut(s) 2325
NmuCI GTSAC 2 cut(s) 1378, 2662
NsiI ATGCAT 3 cut(s) 281, 1482, 2848
NspI RCATGY 1 cut(s) 679
OliI CACNNNNGTG 1 cut(s) 348
PaeI GCATGC 1 cut(s) 679
PaeR7I CTCGAG 1 cut(s) 2063
PagI TCATGA 1 cut(s) 825
PctI GAATGC 2 cut(s) 2697, 2709
PdmI GAANNNNTTC 6 cut(s) 598, 810, 1080, 1512, 1606, 2988
PflMI CCANNNNNTGG 2 cut(s) 1621, 3145
PfoI TCCNGGA 1 cut(s) 3004
PkrI GCNGC 8 cut(s) 100, 184, 342, 454, 642, 871, 1841, 1844
PleI GAGTC 7 cut(s) 202, 1752, 2076, 3096, 3295, 3460, 3481
PpsI GAGTC 7 cut(s) 202, 1752, 2076, 3096, 3295, 3460, 3481
PshAI GACNNNNGTC 1 cut(s) 464
PshBI ATTAAT 2 cut(s) 1895, 2108
Psp124BI GAGCTC 1 cut(s) 366
Psp1406I AACGTT 2 cut(s) 2167, 3541
Psp6I CCWGG 5 cut(s) 443, 979, 1537, 3004, 3279
PspGI CCWGG 5 cut(s) 443, 979, 1537, 3004, 3279
PspN4I GGNNCC 4 cut(s) 327, 344, 601, 668
PspPI GGNCC 4 cut(s) 977, 1501, 1651, 3392
PstI CTGCAG 1 cut(s) 2267
PstNI CAGNNNCTG 1 cut(s) 869
PsuI RGATCY 7 cut(s) 200, 325, 666, 1138, 1442, 1787, 1969
PvuII CAGCTG 1 cut(s) 182
RsaI GTAC 2 cut(s) 2821, 3126
RsaNI GTAC 2 cut(s) 2820, 3125
RseI CAYNNNNRTG 5 cut(s) 128, 348, 593, 1625, 3039
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 8 cut(s) 99, 183, 341, 453, 641, 870, 1840, 1843
Sau96I GGNCC 4 cut(s) 977, 1501, 1651, 3392
SchI GAGTC 7 cut(s) 203, 1752, 2077, 3097, 3296, 3461, 3482
ScrFI CCNGG 7 cut(s) 445, 981, 1505, 1539, 3006, 3281, 3396
SduI GDGCHC 3 cut(s) 366, 1262, 3138
SfaNI GCATC 9 cut(s) 55, 430, 1030, 1467, 2324, 2465, 2833, 2855, 2909
SfcI CTRYAG 2 cut(s) 316, 2263
Sfr274I CTCGAG 1 cut(s) 2063
SinI GGWCC 1 cut(s) 977
SlaI CTCGAG 1 cut(s) 2063
SmiMI CAYNNNNRTG 5 cut(s) 128, 348, 593, 1625, 3039
SmlI CTYRAG 8 cut(s) 146, 1753, 1783, 1958, 2063, 2729, 3368, 3515
SmoI CTYRAG 8 cut(s) 146, 1753, 1783, 1958, 2063, 2729, 3368, 3515
SphI GCATGC 1 cut(s) 679
SsiI CCGC 9 cut(s) 70, 204, 340, 604, 638, 679, 1840, 2557, 3354
SspI AATATT 2 cut(s) 519, 1332
SspMI CTAG 4 cut(s) 198, 1391, 2798, 3387
SstI GAGCTC 1 cut(s) 366
StyD4I CCNGG 7 cut(s) 443, 979, 1503, 1537, 3004, 3279, 3394
StyI CCWWGG 4 cut(s) 670, 1615, 2011, 2434
TaaI ACNGT 5 cut(s) 1070, 1456, 1524, 2381, 3414
TaiI ACGT 3 cut(s) 1287, 2170, 3544
TaqI TCGA 6 cut(s) 1475, 1764, 2064, 2713, 3455, 3476
TaqII GACCGA 1 cut(s) 756
TatI WGTACW 2 cut(s) 2819, 3124
TauI GCSGC 2 cut(s) 343, 1842
TscAI CASTG 4 cut(s) 613, 2181, 2832, 3419
TseFI GTSAC 2 cut(s) 1378, 2662
TseI GCWGC 6 cut(s) 98, 182, 452, 640, 869, 1842
Tsp45I GTSAC 2 cut(s) 1378, 2662
TspGWI ACGGA 4 cut(s) 238, 544, 1167, 3413
TspRI CASTG 4 cut(s) 613, 2181, 2832, 3419
Van91I CCANNNNNTGG 2 cut(s) 1621, 3145
Vha464I CTTAAG 2 cut(s) 1783, 3515
VpaK11BI GGWCC 1 cut(s) 977
VspI ATTAAT 2 cut(s) 1895, 2108
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 1 cut(s) 679
XcmI CCANNNNNNNNNTGG 3 cut(s) 826, 2009, 3041
XhoI CTCGAG 1 cut(s) 2063
XmiI GTMKAC 2 cut(s) 560, 2794
XmnI GAANNNNTTC 6 cut(s) 598, 810, 1080, 1512, 1606, 2988
XspI CTAG 4 cut(s) 198, 1391, 2798, 3387
Zsp2I ATGCAT 3 cut(s) 281, 1482, 2848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.