RchiOBHm_Chr1g0328941

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000105
Physical Location & Seq
Reverse (-)
18401139 .. 18402487
1349 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55829

Sequence Viewer

Length: 1209 bp
ATGTCATCTACCCATGATCGCATAAAATATGTGGGAGGTTCAATCTTTCATGGATGCAAAGTGGTTTGGGGATCTTTAAATAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAAAACAGAGCCAATGAGTTTTGGCTTGCCTATATCTCAAAAAGTTAATACAGAGCCAACGAGTTTCCGCTTGCCTATTTCTGGTCTGTGTAATTTAATGTATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAACTCGGTTACTTTCCCTCGTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGCGTTCCTTTAGGCATTCGATTGCTTTCTAAGCTTGAGAAATTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATTTTTCAATTTCATCAATTGCTTCAATCTAAATGGGAATGAAGGATGCAATAACATAGCATTTGAAATGCTGAAGATATTCATGTATCAGGGAATCTCTAATAAGAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTTAGCCATCAGAGTGTTGGGTGTTCATTAAGTGTATCTCTACCTGTACATTGGAATAACAGTCGGTTTTTGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATGAGCACCATCCGGTGGATAAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTCTATGTTGCCTGAAGCTCGATGGAAAAGAATTGGAAGTGTATGGCAGACAGCCTGCATTTCGCTTCAGTGAAGAATTTTGCCAGGTTGAATCAGATCACCTGTGGCTATTCTATGTATCTTGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAGTCAGCTTGAGTTCTCATTTGAAACCAGATGGCCGGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAAGAAGTGCAAGAGTTGAACCAAACAAACACTCAATCAAGCAGTAGGATGTCTCCTTATGCGGATATATTGATTGGTTTTGACAGTCCAGTTGCAGGGGAAACCAGTGGCACTGGCAGCACAACTTGCATGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

402

Amino Acids

45.6

Weight (kDa)

6.14

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 209 - 358 2.8e-22 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 769
AccI GTMKAC 1 cut(s) 418
AccII CGCG 1 cut(s) 321
AciI CCGC 2 cut(s) 181, 1124
AclWI GGATC 1 cut(s) 79
AcoI YGGCCR 1 cut(s) 1016
AcsI RAATTY 4 cut(s) 359, 475, 791, 896
AcuI CTGAAG 4 cut(s) 590, 854, 871, 1046
AfaI GTAC 4 cut(s) 445, 699, 750, 961
AfiI CCNNNNNNNGG 3 cut(s) 194, 769, 1157
AjnI CCWGG 2 cut(s) 628, 903
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AluBI AGCT 4 cut(s) 352, 778, 838, 991
AluI AGCT 4 cut(s) 352, 778, 838, 991
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 2 cut(s) 376, 1119
AlwI GGATC 1 cut(s) 79
AoxI GGCC 1 cut(s) 1016
ApeKI GCWGC 1 cut(s) 1179
ApoI RAATTY 4 cut(s) 359, 475, 791, 896
Asp700I GAANNNNTTC 2 cut(s) 575, 612
AsuC2I CCSGG 1 cut(s) 1020
AsuHPI GGTGA 4 cut(s) 254, 298, 911, 1042
Bbv12I GWGCWC 1 cut(s) 762
BbvI GCAGC 1 cut(s) 1191
BccI CCATC 6 cut(s) 415, 431, 666, 771, 836, 1008
BciT130I CCWGG 2 cut(s) 630, 905
BcnI CCSGG 1 cut(s) 1020
BcoDI GTCTC 2 cut(s) 376, 1119
BfaI CTAG 1 cut(s) 422
BisI GCNGC 1 cut(s) 1180
BlsI GCNGC 1 cut(s) 1181
Bme1390I CCNGG 3 cut(s) 630, 905, 1020
BmrFI CCNGG 3 cut(s) 630, 905, 1020
BmsI GCATC 4 cut(s) 44, 457, 479, 533
BpuEI CTTGAG 2 cut(s) 374, 1013
BpuMI CCSGG 1 cut(s) 1020
BsaWI WCCGGW 1 cut(s) 766
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 769, 1157
Bse1I ACTGG 3 cut(s) 1151, 1167, 1180
BseBI CCWGG 2 cut(s) 630, 905
BseGI GGATG 4 cut(s) 59, 548, 763, 1116
BseLI CCNNNNNNNGG 3 cut(s) 194, 769, 1157
BseMII CTCAG 2 cut(s) 215, 636
BseNI ACTGG 3 cut(s) 1151, 1167, 1180
BseXI GCAGC 1 cut(s) 1191
Bsh1236I CGCG 1 cut(s) 321
BshFI GGCC 1 cut(s) 1018
BsiHKAI GWGCWC 1 cut(s) 762
BsiSI CCGG 2 cut(s) 767, 1019
BslI CCNNNNNNNGG 3 cut(s) 194, 769, 1157
BsmAI GTCTC 2 cut(s) 376, 1119
BsmI GAATGC 1 cut(s) 333
BsnI GGCC 1 cut(s) 1018
Bsp1286I GDGCHC 1 cut(s) 762
Bsp1407I TGTACA 1 cut(s) 697
Bsp143I GATC 3 cut(s) 16, 71, 916
BspACI CCGC 2 cut(s) 181, 1124
BspANI GGCC 1 cut(s) 1018
BspCNI CTCAG 2 cut(s) 216, 637
BspFNI CGCG 1 cut(s) 321
BspPI GGATC 1 cut(s) 79
BsrGI TGTACA 1 cut(s) 697
BsrI ACTGG 3 cut(s) 1151, 1167, 1180
BssMI GATC 3 cut(s) 16, 71, 916
Bst2UI CCWGG 2 cut(s) 630, 905
Bst4CI ACNGT 3 cut(s) 713, 980, 1148
BstAPI GCANNNNNTGC 2 cut(s) 1069, 1188
BstAUI TGTACA 1 cut(s) 697
BstC8I GCNNGC 4 cut(s) 140, 185, 876, 1193
BstDEI CTNAG 4 cut(s) 224, 299, 348, 645
BstEII GGTNACC 1 cut(s) 286
BstF5I GGATG 4 cut(s) 59, 548, 763, 1116
BstFNI CGCG 1 cut(s) 321
BstKTI GATC 3 cut(s) 19, 74, 919
BstMAI GTCTC 2 cut(s) 376, 1119
BstMBI GATC 3 cut(s) 16, 71, 916
BstMWI GCNNNNNNNGC 5 cut(s) 349, 737, 1069, 1179, 1188
BstNI CCWGG 2 cut(s) 630, 905
BstNSI RCATGY 1 cut(s) 1195
BstPI GGTNACC 1 cut(s) 286
BstSCI CCNGG 3 cut(s) 628, 903, 1018
BstUI CGCG 1 cut(s) 321
BstV1I GCAGC 1 cut(s) 1191
BstX2I RGATCY 1 cut(s) 71
BstYI RGATCY 1 cut(s) 71
BsuRI GGCC 1 cut(s) 1018
BtsCI GGATG 4 cut(s) 59, 548, 763, 1116
BtsIMutI CAGTG 4 cut(s) 449, 895, 1173, 1174
Cac8I GCNNGC 4 cut(s) 140, 185, 876, 1193
Csp6I GTAC 4 cut(s) 444, 698, 749, 960
CviAII CATG 5 cut(s) 14, 50, 580, 755, 1192
CviQI GTAC 4 cut(s) 444, 698, 749, 960
DdeI CTNAG 4 cut(s) 224, 299, 348, 645
DpnI GATC 3 cut(s) 18, 73, 918
DpnII GATC 3 cut(s) 16, 71, 916
DraI TTTAAA 1 cut(s) 78
EaeI YGGCCR 1 cut(s) 1016
Eco57I CTGAAG 4 cut(s) 590, 854, 871, 1046
Eco91I GGTNACC 1 cut(s) 286
EcoO65I GGTNACC 1 cut(s) 286
EcoRI GAATTC 1 cut(s) 791
EcoRII CCWGG 2 cut(s) 628, 903
EcoT22I ATGCAT 1 cut(s) 472
FaeI CATG 5 cut(s) 17, 53, 583, 758, 1195
FatI CATG 5 cut(s) 13, 49, 579, 754, 1191
FblI GTMKAC 1 cut(s) 418
Fnu4HI GCNGC 1 cut(s) 1180
FokI GGATG 4 cut(s) 66, 555, 750, 1123
Fsp4HI GCNGC 1 cut(s) 1180
FspBI CTAG 1 cut(s) 422
GluI GCNGC 1 cut(s) 1180
HaeIII GGCC 1 cut(s) 1018
HapII CCGG 2 cut(s) 767, 1019
Hin1II CATG 5 cut(s) 17, 53, 583, 758, 1195
HindIII AAGCTT 2 cut(s) 350, 776
HinfI GANTC 4 cut(s) 295, 591, 911, 1044
HpaII CCGG 2 cut(s) 767, 1019
HphI GGTGA 4 cut(s) 254, 298, 911, 1042
Hpy166II GTNNAC 1 cut(s) 419
Hpy188I TCNGA 6 cut(s) 219, 400, 663, 916, 1026, 1053
Hpy188III TCNNGA 4 cut(s) 96, 389, 644, 796
Hpy8I GTNNAC 1 cut(s) 419
HpyAV CCTTC 6 cut(s) 102, 239, 413, 533, 1021, 1027
HpyCH4III ACNGT 3 cut(s) 713, 980, 1148
HpyF10VI GCNNNNNNNGC 5 cut(s) 349, 737, 1069, 1179, 1188
HpyF3I CTNAG 4 cut(s) 224, 299, 348, 645
Hsp92II CATG 5 cut(s) 17, 53, 583, 758, 1195
Kzo9I GATC 3 cut(s) 16, 71, 916
LmnI GCTCC 1 cut(s) 248
Lsp1109I GCAGC 1 cut(s) 1191
LweI GCATC 4 cut(s) 44, 457, 479, 533
MaeI CTAG 1 cut(s) 422
MaeIII GTNAC 2 cut(s) 269, 286
MalI GATC 3 cut(s) 18, 73, 918
MboI GATC 3 cut(s) 16, 71, 916
MboII GAAGA 3 cut(s) 583, 812, 905
MfeI CAATTG 1 cut(s) 514
MflI RGATCY 1 cut(s) 71
MhlI GDGCHC 1 cut(s) 762
MlyI GAGTC 1 cut(s) 1053
MnlI CCTC 3 cut(s) 29, 289, 597
Mph1103I ATGCAT 1 cut(s) 472
MroXI GAANNNNTTC 2 cut(s) 575, 612
MseI TTAA 8 cut(s) 77, 159, 209, 363, 428, 488, 680, 804
MslI CAYNNNNRTG 1 cut(s) 663
MspI CCGG 2 cut(s) 767, 1019
MspR9I CCNGG 3 cut(s) 630, 905, 1020
MunI CAATTG 1 cut(s) 514
Mva1269I GAATGC 1 cut(s) 333
MvaI CCWGG 2 cut(s) 630, 905
MvnI CGCG 1 cut(s) 321
MwoI GCNNNNNNNGC 5 cut(s) 349, 737, 1069, 1179, 1188
NciI CCSGG 1 cut(s) 1020
NdeII GATC 3 cut(s) 16, 71, 916
NlaIII CATG 5 cut(s) 17, 53, 583, 758, 1195
NmuCI GTSAC 1 cut(s) 286
NsiI ATGCAT 1 cut(s) 472
NspI RCATGY 1 cut(s) 1195
PaeI GCATGC 1 cut(s) 1195
PctI GAATGC 1 cut(s) 333
PdmI GAANNNNTTC 2 cut(s) 575, 612
PfeI GAWTC 3 cut(s) 295, 591, 911
PflMI CCANNNNNTGG 1 cut(s) 769
PfoI TCCNGGA 1 cut(s) 628
PkrI GCNGC 1 cut(s) 1181
PleI GAGTC 1 cut(s) 1052
PpsI GAGTC 1 cut(s) 1052
Psp6I CCWGG 2 cut(s) 628, 903
PspEI GGTNACC 1 cut(s) 286
PspGI CCWGG 2 cut(s) 628, 903
PsuI RGATCY 1 cut(s) 71
RsaI GTAC 4 cut(s) 445, 699, 750, 961
RsaNI GTAC 4 cut(s) 444, 698, 749, 960
RseI CAYNNNNRTG 1 cut(s) 663
SaqAI TTAA 8 cut(s) 77, 159, 209, 363, 428, 488, 680, 804
SatI GCNGC 1 cut(s) 1180
Sau3AI GATC 3 cut(s) 16, 71, 916
SchI GAGTC 1 cut(s) 1053
ScrFI CCNGG 3 cut(s) 630, 905, 1020
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 4 cut(s) 44, 457, 479, 533
SmiMI CAYNNNNRTG 1 cut(s) 663
SmlI CTYRAG 2 cut(s) 353, 992
SmoI CTYRAG 2 cut(s) 353, 992
SphI GCATGC 1 cut(s) 1195
SsiI CCGC 2 cut(s) 181, 1124
SspI AATATT 1 cut(s) 640
SspMI CTAG 1 cut(s) 422
StyD4I CCNGG 3 cut(s) 628, 903, 1018
TaaI ACNGT 3 cut(s) 713, 980, 1148
TaqI TCGA 2 cut(s) 337, 840
TatI WGTACW 3 cut(s) 443, 697, 748
TfiI GAWTC 3 cut(s) 295, 591, 911
Tru1I TTAA 8 cut(s) 77, 159, 209, 363, 428, 488, 680, 804
Tru9I TTAA 8 cut(s) 77, 159, 209, 363, 428, 488, 680, 804
TscAI CASTG 4 cut(s) 456, 895, 1174, 1180
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 1 cut(s) 1179
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 6 cut(s) 38, 499, 552, 568, 666, 804
TspGWI ACGGA 1 cut(s) 1037
TspRI CASTG 4 cut(s) 456, 895, 1174, 1180
Van91I CCANNNNNTGG 1 cut(s) 769
XapI RAATTY 4 cut(s) 359, 475, 791, 896
XceI RCATGY 1 cut(s) 1195
XcmI CCANNNNNNNNNTGG 1 cut(s) 665
XmiI GTMKAC 1 cut(s) 418
XmnI GAANNNNTTC 2 cut(s) 575, 612
XspI CTAG 1 cut(s) 422
Zsp2I ATGCAT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.