RLG00000015326

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000105
Physical Location & Seq
Reverse (-)
64620183 .. 64626097
5915 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015326

Sequence Viewer

Length: 2418 bp
ATGGAGTCAACCTCTTCTCGTCGTCGACGGAAGTACGATGTGTTTTTAAGTTTCAGAGGGCCAGATGCGCGCAAGGGGATTACCTCCGAGCTGTATCAGGGACTAGAACGGAGAGGAATTGAAACCTTTATGGATGACCCGGATCTTCAGGTAGGAGATTCCATTAGCCCTAAACTTTTGGCTGCAATTGAGGAGTCAAGATTTGCCATTGTTATTCTTTCACCCAAGACCCAACTACGCTTCTTCGCCATGGTGCTTGGAAGAACTCGTCAAGATTATTCAATTTTTGAGCTCAAAAGGAAACCCCGACCCCAAGTGGACGTGGAAGTAAGGGAACATGAAGAAGCTTATGGCAAGACTGAGGATAAATTAAAGGCGTGGAGAGCTGCTTTGACAGAGGTAGCCAATCTTTCTGGGTGGGATTCAAAGAAGTTCAGTACTGATAGAGAACTTGTTGAGGCAATTGTTGGGAAAATTGGGAGTGAGGTGGTGTATAAATCTTCAAGTGTTGACAAGGGCTTCGTAGGAATGGATTCCCGTCTTGATGATTTTTTACGTAAGTACATATATCAAGAGTTGGATGAGGTACGTATTATAGGGATACATGGCATGCGAGGAGTAGGTAAGACAACGCTTGCTCGAGCTTTTTATGATGACTGTTGTCAGGATTTTGAGCATAGATGCTTTCTTCCCAATGTTAGAGAAAGGTTTAAAGGGGGTGGCCTAGTTTCTTTACAAAAGAAGCTTCTTTCCAGCATCTTGATGGACAAAATTGAATACATAGAGGATGAATATACAGGAGCTGCTATGATTCAAAGAAGTTTATGCAAAACAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGGCACAATTAGAAAAATTGGCTGGAAGTCAAGACTGGTTTGGTCCAGGGAGCAGAATTATCATAACCACCACCGATATTCACTTGTTAGAGGCATATGATGTTGATGCTATATACGAGGCTACCACGTTAAATTTTGGTGAGGCTCTTCAACTCTTGAGTTTTAAGGCTTTCAAGAAAAGCTCCCCACCAGAAAATTATTCGGACCTGTGCATCGATATTTTAGAGTATGCTCAGGGGCTGCCGTTAGCTCTTGTGGTTATAGGTTCTTTTCTATGTGGTAGAAGCACCAATGAATGGGCTAGTGCAAGTGATCGGCTAAAAAATACACCAAATAGAGAAATTATTGATGTGCTTCAGATTAGTTTTGATGGTCTAGAGGACATAGAGAAAGAAATATTCCTACATATTGCTTGTCTTTACAAGGGGAAAGATAGGGATCGTGTGAGACAAATACTAGACTATTGTGAGCTGAACCCTGTCATTGGATTAAGAGTTCTAGCTGATAAATCTCTCATTACTACTTCGCACAACGAAGTGTCAATGCATGATTTGCTACAAGAAATGGGCCGGGAAATAGTTCGTCGAGAGTCTCCGAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGAAATCCACAATGTGCTGAAGAAAAATAAGGTGTTCAGAATCTTGAGAGTTTTAGAGCTTGAAGGATGTAAGAGTTTGGTAAGAATCCATCAATCCCTTGGATGTCTCAAAAAGCTTATTGTCCTGAATCTCAAAGACTGCAAAAGTCTTGAGAATCTGCCAGGTAGAATTGAAATGGAATCTCTTGAAAAATTAATTCTTTCTAACTGCTCCAAAATTAAAAAGATTCCCGAGTTTGATGGAAATATGGAGCATCTGTCTGTGCTTCATCTCGACGGGACTGCCATTGAGGGACTGCCTGTTTCAATTGGACGCCTGAGTGGCCTTGTGTCATTGAATCTAAGTAACTGCAGAAATCTTGTTCGTCTTCCAAGCACCATCTTTCATTTATGCAAAGTAGTTTGGGGATCTTTAAATAAGTTCTTGCCTTCTGGATTGCAAAAAGTGAATGGAGAGCCAATGAGTTTCAGCTTGCCTAGGCTGTGTAATTTAACAGATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAATGAATTCAGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGTCTTCCTTCAGGCATTGGGTCGCTTTCCAAGCTTGAGTACTGTAAGTTGAAGAATTGCAAGAGACTTGAAGAGCTGTCAGACCTTCCATTGAATAGTAACCTAGTTTTAAAGGCAGATGATTATGTTTTAGTGATCCTTTCTGGCAATACGGATTTTCACATGCCAGAGAGGTTGGGCGTAGAGGCCAAATGTGATGCCAGCACATTCGGTTCAAGTTGCCTCGGGTGCATGGCAGAATTCGCCGGAAGAAAGCATATTGATCGAGACGACGATCCAAGACTACGGATTGGTATCCTCGCCGGCTATGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

806

Amino Acids

90.68

Weight (kDa)

6.61

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 76 3e-18 TIR domain
TIR_2 PF13676 14 - 76 1.4e-08 TIR domain
NB-ARC PF00931 193 - 350 4.3e-15 NB-ARC domain
WHD_ROQ1 PF23282 418 - 487 3.7e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 505 - 594 9.2e-07 Leucine-rich repeat region
LRR_13 PF23286 561 - 726 1.2e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 582 - 636 1e-05 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1164
AccI GTMKAC 1 cut(s) 25
AccII CGCG 1 cut(s) 70
AclWI GGATC 6 cut(s) 150, 870, 1314, 1918, 2233, 2372
AcsI RAATTY 3 cut(s) 1000, 2048, 2342
AcuI CTGAAG 4 cut(s) 131, 1208, 1541, 2097
AcyI GRCGYC 1 cut(s) 1816
AdeI CACNNNGTG 1 cut(s) 1516
AfaI GTAC 5 cut(s) 35, 439, 563, 588, 2144
AfiI CCNNNNNNNGG 3 cut(s) 1164, 1352, 2412
AjiI CACGTC 1 cut(s) 322
AjnI CCWGG 3 cut(s) 913, 1471, 1663
AloI GAACNNNNNNTCC 2 cut(s) 1347, 1379
Alw21I GWGCWC 1 cut(s) 294
Alw26I GTCTC 6 cut(s) 1309, 1464, 1500, 1613, 2161, 2364
AlwI GGATC 6 cut(s) 150, 870, 1314, 1918, 2233, 2372
AlwNI CAGNNNCTG 2 cut(s) 803, 1108
Ama87I CYCGRG 3 cut(s) 639, 1733, 2327
AoxI GGCC 5 cut(s) 59, 721, 1435, 1825, 2289
ApeKI GCWGC 4 cut(s) 182, 386, 803, 1108
ApoI RAATTY 3 cut(s) 1000, 2048, 2342
ArsI GACNNNNNNTTYG 2 cut(s) 503, 535
AseI ATTAAT 1 cut(s) 1697
Asp700I GAANNNNTTC 2 cut(s) 532, 1446
AspA2I CCTAGG 1 cut(s) 1979
AspLEI GCGC 2 cut(s) 70, 72
AspS9I GGNCC 4 cut(s) 59, 911, 1072, 1435
AsuC2I CCSGG 2 cut(s) 140, 1439
AsuHPI GGTGA 4 cut(s) 213, 1019, 2039, 2083
AvaI CYCGRG 3 cut(s) 639, 1733, 2327
AvaII GGWCC 2 cut(s) 911, 1072
AvrII CCTAGG 1 cut(s) 1979
BanII GRGCYC 1 cut(s) 294
BbsI GAAGAC 2 cut(s) 1862, 2099
Bbv12I GWGCWC 1 cut(s) 294
BbvI GCAGC 4 cut(s) 169, 373, 790, 1095
BccI CCATC 5 cut(s) 757, 1232, 1599, 1736, 1889
BceAI ACGGC 1 cut(s) 1096
BcgI CGANNNNNNTGC 4 cut(s) 1766, 1800, 2348, 2382
BciT130I CCWGG 3 cut(s) 915, 1473, 1665
BciVI GTATCC 2 cut(s) 594, 2408
BcnI CCSGG 2 cut(s) 140, 1439
BcoDI GTCTC 6 cut(s) 1309, 1464, 1500, 1613, 2161, 2364
BfaI CTAG 8 cut(s) 104, 725, 1170, 1244, 1325, 1367, 1980, 2207
BfmI CTRYAG 1 cut(s) 1852
BfuI GTATCC 2 cut(s) 594, 2408
BglI GCCNNNNNGGC 1 cut(s) 1824
BglII AGATCT 1 cut(s) 1999
BisI GCNGC 4 cut(s) 183, 387, 804, 1109
BlnI CCTAGG 1 cut(s) 1979
BlsI GCNGC 4 cut(s) 184, 388, 805, 1110
BmcAI AGTACT 2 cut(s) 439, 2144
Bme1390I CCNGG 5 cut(s) 140, 915, 1439, 1473, 1665
Bme18I GGWCC 2 cut(s) 911, 1072
BmeT110I CYCGRG 3 cut(s) 639, 1733, 2327
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 4 cut(s) 59, 911, 1072, 1435
BmrFI CCNGG 5 cut(s) 140, 915, 1439, 1473, 1665
BmsI GCATC 7 cut(s) 55, 671, 765, 964, 1089, 1765, 2290
BoxI GACNNNNGTC 1 cut(s) 660
BpiI GAAGAC 2 cut(s) 1862, 2099
BplI GAGNNNNNCTC 1 cut(s) 28
Bpu10I CCTNAGC 1 cut(s) 1101
BpuEI CTTGAG 4 cut(s) 1045, 1567, 1673, 2159
BpuMI CCSGG 2 cut(s) 140, 1439
Bsa29I ATCGAT 1 cut(s) 1083
BsaAI YACGTR 2 cut(s) 557, 590
BsaBI GATNNNNATC 3 cut(s) 861, 1305, 2396
BsaHI GRCGYC 1 cut(s) 1816
BsaI GGTCTC 1 cut(s) 1500
BsaJI CCNNGG 5 cut(s) 249, 914, 1600, 1979, 2326
BsaXI ACNNNNNCTCC 2 cut(s) 1745, 1775
Bsc4I CCNNNNNNNGG 3 cut(s) 1164, 1352, 2412
Bse118I RCCGGY 1 cut(s) 2405
Bse1I ACTGG 1 cut(s) 908
Bse8I GATNNNNATC 3 cut(s) 861, 1305, 2396
BseBI CCWGG 3 cut(s) 915, 1473, 1665
BseCI ATCGAT 1 cut(s) 1083
BseDI CCNNGG 5 cut(s) 249, 914, 1600, 1979, 2326
BseGI GGATG 5 cut(s) 139, 586, 793, 1574, 1610
BseJI GATNNNNATC 3 cut(s) 861, 1305, 2396
BseLI CCNNNNNNNGG 3 cut(s) 1164, 1352, 2412
BseMII CTCAG 4 cut(s) 351, 1115, 1811, 2000
BseNI ACTGG 1 cut(s) 908
BsePI GCGCGC 1 cut(s) 68
BseRI GAGGAG 2 cut(s) 206, 630
BseXI GCAGC 4 cut(s) 169, 373, 790, 1095
Bsh1236I CGCG 1 cut(s) 70
BshFI GGCC 5 cut(s) 61, 723, 1437, 1827, 2291
BshVI ATCGAT 1 cut(s) 1083
BsiHKAI GWGCWC 1 cut(s) 294
BsiHKCI CYCGRG 3 cut(s) 639, 1733, 2327
BsiSI CCGG 4 cut(s) 140, 1438, 2349, 2406
BslFI GGGAC 3 cut(s) 114, 1795, 1809
BslI CCNNNNNNNGG 3 cut(s) 1164, 1352, 2412
BsmAI GTCTC 6 cut(s) 1309, 1464, 1500, 1613, 2161, 2364
BsmBI CGTCTC 1 cut(s) 2364
BsmFI GGGAC 3 cut(s) 114, 1795, 1809
BsnI GGCC 5 cut(s) 61, 723, 1437, 1827, 2291
Bso31I GGTCTC 1 cut(s) 1500
BsoBI CYCGRG 3 cut(s) 639, 1733, 2327
Bsp1286I GDGCHC 1 cut(s) 294
Bsp143I GATC 9 cut(s) 142, 862, 1180, 1306, 1910, 1999, 2238, 2365, 2377
Bsp19I CCATGG 1 cut(s) 249
BspANI GGCC 5 cut(s) 61, 723, 1437, 1827, 2291
BspCNI CTCAG 4 cut(s) 352, 1114, 1812, 2001
BspDI ATCGAT 1 cut(s) 1083
BspFNI CGCG 1 cut(s) 70
BspHI TCATGA 1 cut(s) 1498
BspMAI CTGCAG 1 cut(s) 1856
BspPI GGATC 6 cut(s) 150, 870, 1314, 1918, 2233, 2372
BspQI GCTCTTC 2 cut(s) 1020, 2169
BspTNI GGTCTC 1 cut(s) 1500
BsrFI RCCGGY 1 cut(s) 2405
BsrI ACTGG 1 cut(s) 908
BssAI RCCGGY 1 cut(s) 2405
BssECI CCNNGG 5 cut(s) 249, 914, 1600, 1979, 2326
BssHII GCGCGC 1 cut(s) 68
BssMI GATC 9 cut(s) 142, 862, 1180, 1306, 1910, 1999, 2238, 2365, 2377
BssNI GRCGYC 1 cut(s) 1816
BssT1I CCWWGG 3 cut(s) 249, 1600, 1979
Bst2UI CCWGG 3 cut(s) 915, 1473, 1665
Bst4CI ACNGT 2 cut(s) 659, 2147
Bst6I CTCTTC 3 cut(s) 19, 1020, 2169
BstACI GRCGYC 1 cut(s) 1816
BstAPI GCANNNNNTGC 1 cut(s) 1420
BstBAI YACGTR 2 cut(s) 557, 590
BstC8I GCNNGC 6 cut(s) 70, 611, 636, 1976, 2305, 2407
BstDEI CTNAG 6 cut(s) 360, 1101, 1820, 1844, 2009, 2084
BstDSI CCRYGG 1 cut(s) 249
BstEII GGTNACC 1 cut(s) 2071
BstF5I GGATG 5 cut(s) 139, 586, 793, 1574, 1610
BstFNI CGCG 1 cut(s) 70
BstHHI GCGC 2 cut(s) 70, 72
BstKTI GATC 9 cut(s) 145, 865, 1183, 1309, 1913, 2002, 2241, 2368, 2380
BstMAI GTCTC 6 cut(s) 1309, 1464, 1500, 1613, 2161, 2364
BstMBI GATC 9 cut(s) 142, 862, 1180, 1306, 1910, 1999, 2238, 2365, 2377
BstMWI GCNNNNNNNGC 7 cut(s) 67, 383, 1420, 1824, 2134, 2331, 2345
BstNI CCWGG 3 cut(s) 915, 1473, 1665
BstNSI RCATGY 2 cut(s) 613, 2269
BstPAI GACNNNNGTC 1 cut(s) 660
BstPI GGTNACC 1 cut(s) 2071
BstSCI CCNGG 5 cut(s) 138, 913, 1437, 1471, 1663
BstSFI CTRYAG 1 cut(s) 1852
BstSNI TACGTA 2 cut(s) 557, 590
BstUI CGCG 1 cut(s) 70
BstV1I GCAGC 4 cut(s) 169, 373, 790, 1095
BstV2I GAAGAC 2 cut(s) 1862, 2099
BstX2I RGATCY 3 cut(s) 142, 1910, 1999
BstYI RGATCY 3 cut(s) 142, 1910, 1999
Bsu15I ATCGAT 1 cut(s) 1083
BsuI GTATCC 2 cut(s) 594, 2408
BsuRI GGCC 5 cut(s) 61, 723, 1437, 1827, 2291
BsuTUI ATCGAT 1 cut(s) 1083
BtgI CCRYGG 1 cut(s) 249
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 5 cut(s) 139, 586, 793, 1574, 1610
Cac8I GCNNGC 6 cut(s) 70, 611, 636, 1976, 2305, 2407
CaiI CAGNNNCTG 2 cut(s) 803, 1108
CciI TCATGA 1 cut(s) 1498
CfoI GCGC 2 cut(s) 70, 72
Cfr10I RCCGGY 1 cut(s) 2405
Cfr13I GGNCC 4 cut(s) 59, 911, 1072, 1435
ClaI ATCGAT 1 cut(s) 1083
CseI GACGC 1 cut(s) 1824
Csp6I GTAC 5 cut(s) 34, 438, 562, 587, 2143
CviAII CATG 8 cut(s) 250, 338, 605, 610, 1415, 1499, 2266, 2335
CviQI GTAC 5 cut(s) 34, 438, 562, 587, 2143
DdeI CTNAG 6 cut(s) 360, 1101, 1820, 1844, 2009, 2084
DpnI GATC 9 cut(s) 144, 864, 1182, 1308, 1912, 2001, 2240, 2367, 2379
DpnII GATC 9 cut(s) 142, 862, 1180, 1306, 1910, 1999, 2238, 2365, 2377
DraI TTTAAA 3 cut(s) 712, 1917, 2214
DraIII CACNNNGTG 1 cut(s) 1516
Eam1104I CTCTTC 3 cut(s) 19, 1020, 2169
EarI CTCTTC 3 cut(s) 19, 1020, 2169
Ecl136II GAGCTC 1 cut(s) 292
Eco105I TACGTA 2 cut(s) 557, 590
Eco130I CCWWGG 3 cut(s) 249, 1600, 1979
Eco24I GRGCYC 1 cut(s) 294
Eco31I GGTCTC 1 cut(s) 1500
Eco47I GGWCC 2 cut(s) 911, 1072
Eco53kI GAGCTC 1 cut(s) 292
Eco57I CTGAAG 4 cut(s) 131, 1208, 1541, 2097
Eco88I CYCGRG 3 cut(s) 639, 1733, 2327
Eco91I GGTNACC 1 cut(s) 2071
EcoICRI GAGCTC 1 cut(s) 292
EcoO65I GGTNACC 1 cut(s) 2071
EcoRI GAATTC 2 cut(s) 2048, 2342
EcoRII CCWGG 3 cut(s) 913, 1471, 1663
EcoT14I CCWWGG 3 cut(s) 249, 1600, 1979
EcoT22I ATGCAT 1 cut(s) 1416
EcoT38I GRGCYC 1 cut(s) 294
ErhI CCWWGG 3 cut(s) 249, 1600, 1979
Esp3I CGTCTC 1 cut(s) 2364
FaeI CATG 8 cut(s) 253, 341, 608, 613, 1418, 1502, 2269, 2338
FaqI GGGAC 3 cut(s) 114, 1795, 1809
FatI CATG 8 cut(s) 249, 337, 604, 609, 1414, 1498, 2265, 2334
FauNDI CATATG 1 cut(s) 964
FblI GTMKAC 1 cut(s) 25
Fnu4HI GCNGC 4 cut(s) 183, 387, 804, 1109
FokI GGATG 5 cut(s) 146, 593, 800, 1581, 1617
FriOI GRGCYC 1 cut(s) 294
Fsp4HI GCNGC 4 cut(s) 183, 387, 804, 1109
FspBI CTAG 8 cut(s) 104, 725, 1170, 1244, 1325, 1367, 1980, 2207
GlaI GCGC 2 cut(s) 69, 71
GluI GCNGC 4 cut(s) 183, 387, 804, 1109
HaeIII GGCC 5 cut(s) 61, 723, 1437, 1827, 2291
HapII CCGG 4 cut(s) 140, 1438, 2349, 2406
HgaI GACGC 1 cut(s) 1824
HhaI GCGC 2 cut(s) 70, 72
Hin1I GRCGYC 1 cut(s) 1816
Hin1II CATG 8 cut(s) 253, 341, 608, 613, 1418, 1502, 2269, 2338
Hin6I GCGC 2 cut(s) 68, 70
HinP1I GCGC 2 cut(s) 68, 70
HincII GTYRAC 3 cut(s) 9, 26, 511
HindII GTYRAC 3 cut(s) 9, 26, 511
HindIII AAGCTT 4 cut(s) 345, 743, 1616, 2135
HpaII CCGG 4 cut(s) 140, 1438, 2349, 2406
HphI GGTGA 4 cut(s) 213, 1019, 2039, 2083
Hpy166II GTNNAC 4 cut(s) 9, 26, 319, 511
Hpy188I TCNGA 8 cut(s) 56, 88, 1072, 1227, 1464, 1541, 2004, 2185
Hpy8I GTNNAC 4 cut(s) 9, 26, 319, 511
Hpy99I CGWCG 6 cut(s) 24, 27, 30, 1455, 1781, 2378
HpyAV CCTTC 5 cut(s) 1559, 1941, 2024, 2121, 2198
HpyCH4III ACNGT 2 cut(s) 659, 2147
HpyCH4IV ACGT 5 cut(s) 321, 556, 589, 995, 1480
HpyF10VI GCNNNNNNNGC 7 cut(s) 67, 383, 1420, 1824, 2134, 2331, 2345
HpyF3I CTNAG 6 cut(s) 360, 1101, 1820, 1844, 2009, 2084
HpySE526I ACGT 5 cut(s) 321, 556, 589, 995, 1480
Hsp92I GRCGYC 1 cut(s) 1816
Hsp92II CATG 8 cut(s) 253, 341, 608, 613, 1418, 1502, 2269, 2338
HspAI GCGC 2 cut(s) 68, 70
KroI GCCGGC 1 cut(s) 2405
KroNI GCCGGC 1 cut(s) 2407
Kzo9I GATC 9 cut(s) 142, 862, 1180, 1306, 1910, 1999, 2238, 2365, 2377
LguI GCTCTTC 2 cut(s) 1020, 2169
LmnI GCTCC 6 cut(s) 800, 918, 1055, 1718, 1753, 2033
Lsp1109I GCAGC 4 cut(s) 169, 373, 790, 1095
LweI GCATC 7 cut(s) 55, 671, 765, 964, 1089, 1765, 2290
MaeI CTAG 8 cut(s) 104, 725, 1170, 1244, 1325, 1367, 1980, 2207
MaeII ACGT 5 cut(s) 321, 556, 589, 995, 1480
MaeIII GTNAC 4 cut(s) 1847, 2054, 2071, 2201
MalI GATC 9 cut(s) 144, 864, 1182, 1308, 1912, 2001, 2240, 2367, 2379
MboI GATC 9 cut(s) 142, 862, 1180, 1306, 1910, 1999, 2238, 2365, 2377
MfeI CAATTG 3 cut(s) 186, 462, 1809
MflI RGATCY 3 cut(s) 142, 1910, 1999
MhlI GDGCHC 1 cut(s) 294
MlyI GAGTC 3 cut(s) 14, 203, 1466
MmeI TCCRAC 1 cut(s) 558
Mph1103I ATGCAT 1 cut(s) 1416
MroNI GCCGGC 1 cut(s) 2405
MroXI GAANNNNTTC 2 cut(s) 532, 1446
MslI CAYNNNNRTG 1 cut(s) 761
MspI CCGG 4 cut(s) 140, 1438, 2349, 2406
MspR9I CCNGG 5 cut(s) 140, 915, 1439, 1473, 1665
MunI CAATTG 3 cut(s) 186, 462, 1809
MvaI CCWGG 3 cut(s) 915, 1473, 1665
MvnI CGCG 1 cut(s) 70
MwoI GCNNNNNNNGC 7 cut(s) 67, 383, 1420, 1824, 2134, 2331, 2345
NaeI GCCGGC 1 cut(s) 2407
NciI CCSGG 2 cut(s) 140, 1439
NcoI CCATGG 1 cut(s) 249
NdeI CATATG 1 cut(s) 964
NdeII GATC 9 cut(s) 142, 862, 1180, 1306, 1910, 1999, 2238, 2365, 2377
NgoMIV GCCGGC 1 cut(s) 2405
NlaIII CATG 8 cut(s) 253, 341, 608, 613, 1418, 1502, 2269, 2338
NmuCI GTSAC 1 cut(s) 2071
NsiI ATGCAT 1 cut(s) 1416
NspI RCATGY 2 cut(s) 613, 2269
PaeI GCATGC 1 cut(s) 613
PaeR7I CTCGAG 1 cut(s) 639
PagI TCATGA 1 cut(s) 1498
PauI GCGCGC 1 cut(s) 68
PciSI GCTCTTC 2 cut(s) 1020, 2169
PdiI GCCGGC 1 cut(s) 2407
PdmI GAANNNNTTC 2 cut(s) 532, 1446
PflMI CCANNNNNTGG 1 cut(s) 1164
PkrI GCNGC 4 cut(s) 184, 388, 805, 1110
PleI GAGTC 3 cut(s) 13, 202, 1465
PpsI GAGTC 3 cut(s) 13, 202, 1465
Ppu21I YACGTR 2 cut(s) 557, 590
PshAI GACNNNNGTC 1 cut(s) 660
PshBI ATTAAT 1 cut(s) 1697
Psp124BI GAGCTC 1 cut(s) 294
Psp6I CCWGG 3 cut(s) 913, 1471, 1663
PspEI GGTNACC 1 cut(s) 2071
PspGI CCWGG 3 cut(s) 913, 1471, 1663
PspPI GGNCC 4 cut(s) 59, 911, 1072, 1435
PspXI VCTCGAGB 1 cut(s) 639
PstI CTGCAG 1 cut(s) 1856
PstNI CAGNNNCTG 2 cut(s) 803, 1108
PsuI RGATCY 3 cut(s) 142, 1910, 1999
PteI GCGCGC 1 cut(s) 68
RsaI GTAC 5 cut(s) 35, 439, 563, 588, 2144
RsaNI GTAC 5 cut(s) 34, 438, 562, 587, 2143
RseI CAYNNNNRTG 1 cut(s) 761
SacI GAGCTC 1 cut(s) 294
SalI GTCGAC 1 cut(s) 24
SapI GCTCTTC 2 cut(s) 1020, 2169
SatI GCNGC 4 cut(s) 183, 387, 804, 1109
Sau3AI GATC 9 cut(s) 142, 862, 1180, 1306, 1910, 1999, 2238, 2365, 2377
Sau96I GGNCC 4 cut(s) 59, 911, 1072, 1435
ScaI AGTACT 2 cut(s) 439, 2144
SchI GAGTC 3 cut(s) 14, 203, 1466
ScrFI CCNGG 5 cut(s) 140, 915, 1439, 1473, 1665
SduI GDGCHC 1 cut(s) 294
SfaNI GCATC 7 cut(s) 55, 671, 765, 964, 1089, 1765, 2290
SfcI CTRYAG 1 cut(s) 1852
Sfr274I CTCGAG 1 cut(s) 639
SgrDI CGTCGACG 1 cut(s) 24
SinI GGWCC 2 cut(s) 911, 1072
SlaI CTCGAG 1 cut(s) 639
SmiMI CAYNNNNRTG 1 cut(s) 761
SmlI CTYRAG 5 cut(s) 639, 1024, 1546, 1652, 2138
SmoI CTYRAG 5 cut(s) 639, 1024, 1546, 1652, 2138
SnaBI TACGTA 2 cut(s) 557, 590
SphI GCATGC 1 cut(s) 613
SspI AATATT 1 cut(s) 1266
SspMI CTAG 8 cut(s) 104, 725, 1170, 1244, 1325, 1367, 1980, 2207
SstI GAGCTC 1 cut(s) 294
StyD4I CCNGG 5 cut(s) 138, 913, 1437, 1471, 1663
StyI CCWWGG 3 cut(s) 249, 1600, 1979
TaaI ACNGT 2 cut(s) 659, 2147
TaiI ACGT 5 cut(s) 324, 559, 592, 998, 1483
TaqI TCGA 6 cut(s) 25, 640, 1083, 1453, 1776, 2368
TatI WGTACW 3 cut(s) 437, 561, 2142
TseFI GTSAC 1 cut(s) 2071
TseI GCWGC 4 cut(s) 182, 386, 803, 1108
Tsp45I GTSAC 1 cut(s) 2071
TspDTI ATGAA 7 cut(s) 354, 804, 1176, 1515, 1760, 1877, 2061
TspGWI ACGGA 4 cut(s) 43, 124, 2270, 2404
Van91I CCANNNNNTGG 1 cut(s) 1164
VpaK11BI GGWCC 2 cut(s) 911, 1072
VspI ATTAAT 1 cut(s) 1697
XapI RAATTY 3 cut(s) 1000, 2048, 2342
XbaI TCTAGA 1 cut(s) 1243
XceI RCATGY 2 cut(s) 613, 2269
XcmI CCANNNNNNNNNTGG 2 cut(s) 760, 1598
XhoI CTCGAG 1 cut(s) 639
XmaJI CCTAGG 1 cut(s) 1979
XmiI GTMKAC 1 cut(s) 25
XmnI GAANNNNTTC 2 cut(s) 532, 1446
XspI CTAG 8 cut(s) 104, 725, 1170, 1244, 1325, 1367, 1980, 2207
ZrmI AGTACT 2 cut(s) 439, 2144
Zsp2I ATGCAT 1 cut(s) 1416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.