Rroxscaffold_160G00433940

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000160
OG0000105
Physical Location & Seq
Reverse (-)
457412 .. 467768
10357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_160G00433940.1

Sequence Viewer

Length: 1872 bp
ATGGACTGGGTGTCCCTTAAGATCTCTCCCAAAAAATTTAAAGCACATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTAAAAAGCTATGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTAAAAAAATTGTGGAGAGCCCCGACCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGACGATTCCCAAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTCGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTGGCCTTGTGTCATTGAATGTAAGCAACTGCAGAAAACTTGTTTGTCTTCCAAGCACCGTCAAGAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAGCATCAGGTAAATATGGAGGAGATGGACTGTTTTGAGGAAACTGATGTGAAGAGTGGGTATGCAATAGAAATGTCATCTACCCTTGATCGCATAAAACATGTGAGAGGTTTAATCTTTCATCGATGCAAAGTAGTTTGGGGATCTTTAAATAAGTTTTTGCCTTCTGGATTGGTGCAAAAAGTGAATACACAGCCAATGAGTTTCTGCTTGCCTATATCTCAAAATGGGAATACAGAGCCAACGAGTTTAGGCTTGCCTATATCTCAAAAAGTGAATACAGAGCCAACGAGTTTCCGCTTGCCTATTTCTGGTCTGTGTAATTTAACGTATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACAAATTCGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGCTTTCCTTCAGGCATTCGATTGCTTTCTAAACTTGAGAACTTTAACTTGGAGAATTGTAAGAGACTTAAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAATAGATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATGAAGGATGCAATAACATAGCATTTGAAATGCTGAGGACATTCATGTATCAGGGAATCTCTAATAAGAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCAGAGTGTTGGGTGTTCATTAAGTGTATCTCTACCTGTCCATTGGAATAACAGTCAGTTTTTGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATGAGCACCATCCGGTGGATGAGCTTTATACAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCAATGGAAGAGAATTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTAAATCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAGTCAGCTTGAGTTCTTATTTGAAACCAGAGGGCCGGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAAGAAGTGCAAGAGTTGAACCAAACAACCACTCAATCAAGCAGTAGGATGTCTCAAGAGGATATATTGGTTGGCACTGGTAGCAGAACTTGCACGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

623

Amino Acids

70.78

Weight (kDa)

8.17

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_3 PF07725 18 - 36 2.1e-06 Leucine Rich Repeat
LRR_13 PF23286 109 - 194 4.6e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 120 - 190 6.2e-09 Leucine-rich repeat region
C-JID PF20160 442 - 591 3.2e-21 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 167
Acc36I ACCTGC 1 cut(s) 167
AccB7I CCANNNNNTGG 1 cut(s) 1468
AccI GTMKAC 1 cut(s) 1117
AciI CCGC 1 cut(s) 880
AclI AACGTT 1 cut(s) 400
AclWI GGATC 1 cut(s) 733
AcsI RAATTY 6 cut(s) 35, 109, 962, 1174, 1490, 1595
AcuI CTGAAG 3 cut(s) 1011, 1553, 1745
AfaI GTAC 3 cut(s) 1144, 1449, 1660
AfiI CCNNNNNNNGG 2 cut(s) 893, 1468
AflII CTTAAG 1 cut(s) 17
AflIII ACRYGT 1 cut(s) 682
AhdI GACNNNNNGTC 1 cut(s) 10
AjnI CCWGG 2 cut(s) 1327, 1602
AjuI GAANNNNNNNTTGG 2 cut(s) 1049, 1081
AluBI AGCT 5 cut(s) 91, 262, 1477, 1537, 1690
AluI AGCT 5 cut(s) 91, 262, 1477, 1537, 1690
Alw21I GWGCWC 1 cut(s) 1461
Alw26I GTCTC 6 cut(s) 188, 299, 419, 422, 1075, 1818
AlwI GGATC 1 cut(s) 733
Ama87I CYCGRG 1 cut(s) 296
AoxI GGCC 2 cut(s) 469, 1715
ApoI RAATTY 6 cut(s) 35, 109, 962, 1174, 1490, 1595
AseI ATTAAT 1 cut(s) 341
Asp700I GAANNNNTTC 1 cut(s) 1311
AspS9I GGNCC 1 cut(s) 1715
AsuC2I CCSGG 1 cut(s) 1719
AsuHPI GGTGA 4 cut(s) 953, 997, 1610, 1741
AvaI CYCGRG 1 cut(s) 296
BanII GRGCYC 1 cut(s) 167
BauI CACGAG 1 cut(s) 1641
BbsI GAAGAC 1 cut(s) 506
Bbv12I GWGCWC 1 cut(s) 1461
BbvCI CCTCAGC 1 cut(s) 1268
BccI CCATC 6 cut(s) 243, 601, 1114, 1130, 1365, 1470
BceAI ACGGC 1 cut(s) 476
BcgI CGANNNNNNTGC 4 cut(s) 277, 311, 410, 444
BciT130I CCWGG 2 cut(s) 1329, 1604
BcnI CCSGG 1 cut(s) 1719
BcoDI GTCTC 6 cut(s) 188, 299, 419, 422, 1075, 1818
BfaI CTAG 1 cut(s) 1121
BfmI CTRYAG 1 cut(s) 496
BfrI CTTAAG 1 cut(s) 17
BfuAI ACCTGC 1 cut(s) 167
BglII AGATCT 2 cut(s) 21, 202
Bme1390I CCNGG 3 cut(s) 1329, 1604, 1719
BmeRI GACNNNNNGTC 1 cut(s) 10
BmeT110I CYCGRG 1 cut(s) 296
BmgT120I GGNCC 1 cut(s) 1715
BmrFI CCNGG 3 cut(s) 1329, 1604, 1719
BmrI ACTGGG 1 cut(s) 16
BmsI GCATC 6 cut(s) 557, 595, 698, 1156, 1178, 1232
BmuI ACTGGG 1 cut(s) 16
BpiI GAAGAC 1 cut(s) 506
Bpu10I CCTNAGC 1 cut(s) 1268
BpuEI CTTGAG 4 cut(s) 212, 1073, 1712, 1800
BpuMI CCSGG 1 cut(s) 1719
Bsa29I ATCGAT 1 cut(s) 706
BsaJI CCNNGG 1 cut(s) 244
BsaWI WCCGGW 1 cut(s) 1465
Bsc4I CCNNNNNNNGG 2 cut(s) 893, 1468
Bse1I ACTGG 3 cut(s) 11, 472, 1843
Bse3DI GCAATG 1 cut(s) 1240
BseBI CCWGG 2 cut(s) 1329, 1604
BseCI ATCGAT 1 cut(s) 706
BseDI CCNNGG 1 cut(s) 244
BseGI GGATG 6 cut(s) 218, 572, 1247, 1462, 1477, 1815
BseLI CCNNNNNNNGG 2 cut(s) 893, 1468
BseMI GCAATG 1 cut(s) 1240
BseMII CTCAG 3 cut(s) 914, 1259, 1335
BseNI ACTGG 3 cut(s) 11, 472, 1843
BseRI GAGGAG 1 cut(s) 617
BshFI GGCC 2 cut(s) 471, 1717
BshVI ATCGAT 1 cut(s) 706
BsiHKAI GWGCWC 1 cut(s) 1461
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 2 cut(s) 1466, 1718
BslI CCNNNNNNNGG 2 cut(s) 893, 1468
BsmAI GTCTC 6 cut(s) 188, 299, 419, 422, 1075, 1818
BsmI GAATGC 1 cut(s) 1032
BsnI GGCC 2 cut(s) 471, 1717
BsoBI CYCGRG 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 167, 1461
Bsp143I GATC 6 cut(s) 21, 202, 670, 725, 1193, 1615
BspACI CCGC 1 cut(s) 880
BspANI GGCC 2 cut(s) 471, 1717
BspCNI CTCAG 3 cut(s) 915, 1260, 1336
BspDI ATCGAT 1 cut(s) 706
BspMAI CTGCAG 1 cut(s) 500
BspMI ACCTGC 1 cut(s) 167
BspPI GGATC 1 cut(s) 733
BspTI CTTAAG 1 cut(s) 17
BsrDI GCAATG 1 cut(s) 1240
BsrI ACTGG 3 cut(s) 11, 472, 1843
BssECI CCNNGG 1 cut(s) 244
BssMI GATC 6 cut(s) 21, 202, 670, 725, 1193, 1615
BssSI CACGAG 1 cut(s) 1641
BssT1I CCWWGG 1 cut(s) 244
Bst2BI CACGAG 1 cut(s) 1641
Bst2UI CCWGG 2 cut(s) 1329, 1604
Bst4CI ACNGT 4 cut(s) 526, 614, 1412, 1679
Bst6I CTCTTC 2 cut(s) 629, 1540
BstAFI CTTAAG 1 cut(s) 17
BstAPI GCANNNNNTGC 2 cut(s) 1768, 1851
BstC8I GCNNGC 6 cut(s) 584, 794, 839, 884, 1575, 1856
BstDEI CTNAG 4 cut(s) 923, 998, 1268, 1344
BstEII GGTNACC 1 cut(s) 985
BstF5I GGATG 6 cut(s) 218, 572, 1247, 1462, 1477, 1815
BstKTI GATC 6 cut(s) 24, 205, 673, 728, 1196, 1618
BstMAI GTCTC 6 cut(s) 188, 299, 419, 422, 1075, 1818
BstMBI GATC 6 cut(s) 21, 202, 670, 725, 1193, 1615
BstMWI GCNNNNNNNGC 4 cut(s) 1436, 1768, 1842, 1851
BstNI CCWGG 2 cut(s) 1329, 1604
BstNSI RCATGY 1 cut(s) 686
BstPI GGTNACC 1 cut(s) 985
BstSCI CCNGG 3 cut(s) 1327, 1602, 1717
BstSFI CTRYAG 1 cut(s) 496
BstV2I GAAGAC 1 cut(s) 506
BstX2I RGATCY 3 cut(s) 21, 202, 725
BstYI RGATCY 3 cut(s) 21, 202, 725
Bsu15I ATCGAT 1 cut(s) 706
BsuRI GGCC 2 cut(s) 471, 1717
BsuTUI ATCGAT 1 cut(s) 706
BtsCI GGATG 6 cut(s) 218, 572, 1247, 1462, 1477, 1815
BtsIMutI CAGTG 2 cut(s) 1148, 1836
BveI ACCTGC 1 cut(s) 167
Cac8I GCNNGC 6 cut(s) 584, 794, 839, 884, 1575, 1856
Cfr13I GGNCC 1 cut(s) 1715
ClaI ATCGAT 1 cut(s) 706
Csp6I GTAC 3 cut(s) 1143, 1448, 1659
CviAII CATG 4 cut(s) 47, 683, 1279, 1454
CviQI GTAC 3 cut(s) 1143, 1448, 1659
DdeI CTNAG 4 cut(s) 923, 998, 1268, 1344
DpnI GATC 6 cut(s) 23, 204, 672, 727, 1195, 1617
DpnII GATC 6 cut(s) 21, 202, 670, 725, 1193, 1615
DraI TTTAAA 2 cut(s) 40, 732
DriI GACNNNNNGTC 1 cut(s) 10
Eam1104I CTCTTC 2 cut(s) 629, 1540
Eam1105I GACNNNNNGTC 1 cut(s) 10
EarI CTCTTC 2 cut(s) 629, 1540
Eco130I CCWWGG 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 167
Eco57I CTGAAG 3 cut(s) 1011, 1553, 1745
Eco88I CYCGRG 1 cut(s) 296
Eco91I GGTNACC 1 cut(s) 985
EcoO65I GGTNACC 1 cut(s) 985
EcoRI GAATTC 1 cut(s) 1490
EcoRII CCWGG 2 cut(s) 1327, 1602
EcoT14I CCWWGG 1 cut(s) 244
EcoT22I ATGCAT 1 cut(s) 1171
EcoT38I GRGCYC 1 cut(s) 167
ErhI CCWWGG 1 cut(s) 244
FaeI CATG 4 cut(s) 50, 686, 1282, 1457
FatI CATG 4 cut(s) 46, 682, 1278, 1453
FblI GTMKAC 1 cut(s) 1117
FokI GGATG 6 cut(s) 225, 579, 1254, 1449, 1484, 1822
FriOI GRGCYC 1 cut(s) 167
FspBI CTAG 1 cut(s) 1121
HaeIII GGCC 2 cut(s) 471, 1717
HapII CCGG 2 cut(s) 1466, 1718
Hin1II CATG 4 cut(s) 50, 686, 1282, 1457
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 9 cut(s) 187, 231, 274, 301, 326, 373, 994, 1290, 1743
HpaII CCGG 2 cut(s) 1466, 1718
HphI GGTGA 4 cut(s) 953, 997, 1610, 1741
Hpy166II GTNNAC 1 cut(s) 1118
Hpy188I TCNGA 7 cut(s) 186, 918, 1099, 1362, 1615, 1725, 1752
Hpy8I GTNNAC 1 cut(s) 1118
HpyAV CCTTC 9 cut(s) 91, 203, 756, 938, 1035, 1112, 1232, 1720, 1726
HpyCH4III ACNGT 4 cut(s) 526, 614, 1412, 1679
HpyCH4IV ACGT 2 cut(s) 400, 911
HpyF10VI GCNNNNNNNGC 4 cut(s) 1436, 1768, 1842, 1851
HpyF3I CTNAG 4 cut(s) 923, 998, 1268, 1344
HpySE526I ACGT 2 cut(s) 400, 911
Hsp92II CATG 4 cut(s) 50, 686, 1282, 1457
Kzo9I GATC 6 cut(s) 21, 202, 670, 725, 1193, 1615
LmnI GCTCC 1 cut(s) 947
LweI GCATC 6 cut(s) 557, 595, 698, 1156, 1178, 1232
MaeI CTAG 1 cut(s) 1121
MaeII ACGT 2 cut(s) 400, 911
MaeIII GTNAC 2 cut(s) 968, 985
MalI GATC 6 cut(s) 23, 204, 672, 727, 1195, 1617
MboI GATC 6 cut(s) 21, 202, 670, 725, 1193, 1615
MboII GAAGA 4 cut(s) 506, 646, 1557, 1604
MfeI CAATTG 2 cut(s) 453, 1213
MflI RGATCY 3 cut(s) 21, 202, 725
MhlI GDGCHC 2 cut(s) 167, 1461
MlyI GAGTC 2 cut(s) 310, 1752
MmeI TCCRAC 1 cut(s) 367
Mph1103I ATGCAT 1 cut(s) 1171
MroXI GAANNNNTTC 1 cut(s) 1311
MslI CAYNNNNRTG 1 cut(s) 1362
MspCI CTTAAG 1 cut(s) 17
MspI CCGG 2 cut(s) 1466, 1718
MspR9I CCNGG 3 cut(s) 1329, 1604, 1719
MunI CAATTG 2 cut(s) 453, 1213
Mva1269I GAATGC 1 cut(s) 1032
MvaI CCWGG 2 cut(s) 1329, 1604
MwoI GCNNNNNNNGC 4 cut(s) 1436, 1768, 1842, 1851
NciI CCSGG 1 cut(s) 1719
NdeII GATC 6 cut(s) 21, 202, 670, 725, 1193, 1615
NlaIII CATG 4 cut(s) 50, 686, 1282, 1457
NmeAIII GCCGAG 1 cut(s) 558
NmuCI GTSAC 1 cut(s) 985
NsiI ATGCAT 1 cut(s) 1171
NspI RCATGY 1 cut(s) 686
PaeR7I CTCGAG 1 cut(s) 296
PaqCI CACCTGC 1 cut(s) 167
PciI ACATGT 1 cut(s) 682
PctI GAATGC 1 cut(s) 1032
PdmI GAANNNNTTC 1 cut(s) 1311
PfeI GAWTC 7 cut(s) 187, 231, 274, 326, 373, 994, 1290
PflMI CCANNNNNTGG 1 cut(s) 1468
PfoI TCCNGGA 1 cut(s) 1327
PleI GAGTC 2 cut(s) 309, 1751
PpsI GAGTC 2 cut(s) 309, 1751
PscI ACATGT 1 cut(s) 682
PshBI ATTAAT 1 cut(s) 341
Psp1406I AACGTT 1 cut(s) 400
Psp6I CCWGG 2 cut(s) 1327, 1602
PspEI GGTNACC 1 cut(s) 985
PspGI CCWGG 2 cut(s) 1327, 1602
PspPI GGNCC 1 cut(s) 1715
PstI CTGCAG 1 cut(s) 500
PsuI RGATCY 3 cut(s) 21, 202, 725
RsaI GTAC 3 cut(s) 1144, 1449, 1660
RsaNI GTAC 3 cut(s) 1143, 1448, 1659
RseI CAYNNNNRTG 1 cut(s) 1362
Sau3AI GATC 6 cut(s) 21, 202, 670, 725, 1193, 1615
Sau96I GGNCC 1 cut(s) 1715
SchI GAGTC 2 cut(s) 310, 1752
ScrFI CCNGG 3 cut(s) 1329, 1604, 1719
SduI GDGCHC 2 cut(s) 167, 1461
SfaNI GCATC 6 cut(s) 557, 595, 698, 1156, 1178, 1232
SfcI CTRYAG 1 cut(s) 496
Sfr274I CTCGAG 1 cut(s) 296
SlaI CTCGAG 1 cut(s) 296
SmiMI CAYNNNNRTG 1 cut(s) 1362
SmlI CTYRAG 6 cut(s) 17, 191, 296, 1052, 1691, 1815
SmoI CTYRAG 6 cut(s) 17, 191, 296, 1052, 1691, 1815
SsiI CCGC 1 cut(s) 880
SspI AATATT 1 cut(s) 1339
SspMI CTAG 1 cut(s) 1121
StyD4I CCNGG 3 cut(s) 1327, 1602, 1717
StyI CCWWGG 1 cut(s) 244
TaaI ACNGT 4 cut(s) 526, 614, 1412, 1679
TaiI ACGT 2 cut(s) 403, 914
TaqI TCGA 4 cut(s) 297, 420, 706, 1036
TatI WGTACW 2 cut(s) 1142, 1447
TfiI GAWTC 7 cut(s) 187, 231, 274, 326, 373, 994, 1290
TscAI CASTG 2 cut(s) 1155, 1843
TseFI GTSAC 1 cut(s) 985
Tsp45I GTSAC 1 cut(s) 985
TspDTI ATGAA 8 cut(s) 63, 118, 692, 1198, 1251, 1267, 1365, 1503
TspGWI ACGGA 1 cut(s) 1736
TspRI CASTG 2 cut(s) 1155, 1843
Van91I CCANNNNNTGG 1 cut(s) 1468
Vha464I CTTAAG 1 cut(s) 17
VspI ATTAAT 1 cut(s) 341
XapI RAATTY 6 cut(s) 35, 109, 962, 1174, 1490, 1595
XceI RCATGY 1 cut(s) 686
XcmI CCANNNNNNNNNTGG 2 cut(s) 242, 1364
XhoI CTCGAG 1 cut(s) 296
XmiI GTMKAC 1 cut(s) 1117
XmnI GAANNNNTTC 1 cut(s) 1311
XspI CTAG 1 cut(s) 1121
Zsp2I ATGCAT 1 cut(s) 1171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.