Rroxscaffold_4G00326500

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Reverse (-)
58656335 .. 58662782
6448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326500.1

Sequence Viewer

Length: 3618 bp
ATGGTGATCATTTCCGTGAACAACACAACAAGCGAGTCTGATTCAATTCCGATTAGGAGATCGATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTGTTTCTAAGTTTCAGGGGGGATGATACCCGCAAGGGTTTTACTGGTCGCCTGTATTCTGCACTAAATCAGAGAGGAATTCTCACCTTTATGGATGAGCCAGAGCTTGAGAAAGGAAAATCCATTAGGCCTGAACTTTTAGCTGCAATTGAGGACTCTAGATCTGCCATTGTCATTCTCTCATGGAGATATGCTGAGTCGTCATGGTGCTTGGAAGAACTCGTCAAGATTATTCAATGCATGAAAGAGATGGGCCAACAAGTCCTTCCCGTCTTCTACTGGGTGGATCCTTCAGATGTGCGGCACCAAAGGAGATGGTTTGAGCTCAAACATTGGCAACCCAAAGTAGAAGTAGAAGTAAGGGAGCATGAAGAAGTTTATCGGGATACTCCGATAAACTGCTTGGACAGACATGCTGCTTGGAGAGCTGCTTTGACTGAGGTCGCCAATCTCTCTGGCTGGGATGCAAAGAATACTCCATCTTATGCAGAACTAATTGACGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTCATGATCTATTAAGCAATTACATATGTCCACAGCTTGTTGGGGTGCGTTTTGTAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATCAAATTTGTCAAAATTATGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCCTAGTTTCTGTACAAGAAAAACTTCTTTCTAGAATCCTAATGGCAAAAATTGAAAATATAGAGGATGAATATACAGGAGCTGCTATGATAGAAAGGCGGCTGTGTAGAAGAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGAAGTAGAATCATCATAACCACCACAGATATTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTATTTGGTTAAACTATGCTGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATGTCCCCCACCAGAAGATTATTTGCATCTGTGCCACCATATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCGGTTGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCACTGATAAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTAACGTGCTTCGGGTTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAGACCCTGTCATTGGTTTAGATGTACTTGCTGATAGATCTCTCATAACTATCTCCAACAACAAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAACAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAGACATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTGAATTACAAGTGGCTCATTGGAAGCCAGAATCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTCGTAATGTGGACCTTCCTGAAGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTAAGATCTCTCCCACAAAATTTTGAAGCAGATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTGAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCACAGGCGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGCGTCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTTGAGAGTCTGCCTAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCCAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGAAACGTTTGTTGGTGCTTAGTCTTGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTGGCCTTGTGTCATTGAATCTAAGCAACTGCAGAAATCTTGTATGTCTTCCAAGCACCATCAATAAATTGAAGTCTGTTGAAGATCTTAATCTTTCTGGATGCTTGAAACTTGGCAAACATCAGGTAAATGTGGGGGAGATGGACTGTTTTGAGGAAAATGATGTGAGTAGTGGGTCTGCAATAGAAATCTCATCTGCCCGTGATCGCATAAAATATGTGAGAGGTTCAGTCTTTCGTGGATGCGAAGTAGTTTGGGGATCTGTAAATAAGTTCTTGCCTTCAGGATTGGTGCAAAAAGTGAATACAGAGCCAATGAGTTTCCGCTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGTCTTCCTTCTGGCATTAGATTGCTTTCTAAGCTTGAGAACTTCAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATTTATCAGAGAATCTTTAATAACAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAAGATTCCTAGGTGGTTCAGCCATCAGAGAGTTGGGTGTTCCTTAAGTGTATCTCTACCTGCAAATTGGAATGACAGTCTGTTTTTGGGATTTGCTTTCTGTGCTGTTTTTGTACTCCATGAGCACCATCGGGTGGATAAGCTTCATAGAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCGATGGAAGAGAATTGGAAGTATATGGCAGACACCCTGCATTTCGCTTTAGTGAAGAATTTTGCAAGGTTAAGTCAGATCACCTGTGGCTATTCTATGTATCTCGTGGTGAATACTTTGGTCCAAAGTGGTGGCTTAACGGTTGCAGTCAGCTTGAGTTCTTATTTAAAACCAAAGGACCGGGTCTGGAGTTGAAGAAGTGTGGAGTCCGTCTGATATATGTGCAAGATGAGCAAGAGTTGAACCAAACAATCACTCAATCAAACAGTAGGACTGGCAGAATGACGGGGGATCCACCACTGGCCACTCTGAAAGACCACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1205

Amino Acids

137.43

Weight (kDa)

7.48

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 32 - 217 1.1e-44 TIR domain
TIR_2 PF13676 35 - 146 3.8e-14 TIR domain
NB-ARC PF00931 243 - 397 1.4e-18 NB-ARC domain
NACHT PF05729 246 - 343 1.9e-07 NACHT domain
WHD_ROQ1 PF23282 464 - 535 2.7e-23 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 633 - 651 3.3e-06 Leucine Rich Repeat
LRR_14 PF23598 694 - 804 7.6e-10 Leucine-rich repeat region
LRR_13 PF23286 724 - 809 1.1e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 1027 - 1175 9.8e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 833, 3157
AccB1I GGYRCC 1 cut(s) 405
AccB7I CCANNNNNTGG 2 cut(s) 1699, 3223
AccI GTMKAC 2 cut(s) 638, 2872
AciI CCGC 7 cut(s) 133, 403, 682, 757, 964, 1262, 2635
AclI AACGTT 1 cut(s) 2245
AclWI GGATC 9 cut(s) 383, 396, 739, 752, 1014, 1458, 2578, 3578, 3591
AcoI YGGCCR 1 cut(s) 3594
AcuI CTGAAG 7 cut(s) 378, 1173, 1685, 1824, 2577, 3044, 3308
AcyI GRCGYC 1 cut(s) 68
AfaI GTAC 4 cut(s) 879, 1509, 2899, 3204
AfiI CCNNNNNNNGG 4 cut(s) 1496, 1699, 2648, 3223
AflII CTTAAG 2 cut(s) 1861, 3133
AjnI CCWGG 3 cut(s) 1057, 1615, 3082
AjuI GAANNNNNNNTTGG 6 cut(s) 351, 383, 2234, 2266, 2804, 2836
Alw21I GWGCWC 2 cut(s) 429, 3216
Alw26I GTCTC 8 cut(s) 1608, 1644, 1663, 1767, 1828, 2000, 2264, 2830
AlwI GGATC 9 cut(s) 383, 396, 739, 752, 1014, 1458, 2578, 3578, 3591
AlwNI CAGNNNCTG 1 cut(s) 947
Ama87I CYCGRG 2 cut(s) 783, 2222
AoxI GGCC 6 cut(s) 230, 355, 865, 1807, 2314, 3594
ApeKI GCWGC 4 cut(s) 245, 518, 530, 947
AseI ATTAAT 1 cut(s) 2186
Asp700I GAANNNNTTC 7 cut(s) 676, 888, 1158, 1590, 1684, 3029, 3066
AspA2I CCTAGG 1 cut(s) 3098
AspS9I GGNCC 5 cut(s) 355, 1055, 1795, 3413, 3470
AsuC2I CCSGG 1 cut(s) 3474
AsuHPI GGTGA 6 cut(s) 16, 178, 2708, 2752, 3365, 3413
AvaI CYCGRG 2 cut(s) 783, 2222
AvaII GGWCC 4 cut(s) 1055, 1795, 3413, 3470
AvrII CCTAGG 1 cut(s) 3098
BalI TGGCCA 1 cut(s) 3596
BamHI GGATCC 3 cut(s) 388, 744, 3583
BanI GGYRCC 1 cut(s) 405
BanII GRGCYC 1 cut(s) 429
BauI CACGAG 1 cut(s) 3396
BbsI GAAGAC 6 cut(s) 367, 1653, 1766, 2351, 2768, 3032
Bbv12I GWGCWC 2 cut(s) 429, 3216
BbvI GCAGC 4 cut(s) 232, 505, 517, 934
BceAI ACGGC 1 cut(s) 2321
BciT130I CCWGG 3 cut(s) 1059, 1617, 3084
BciVI GTATCC 2 cut(s) 481, 1866
BclI TGATCA 2 cut(s) 6, 796
BcnI CCSGG 1 cut(s) 3474
BcoDI GTCTC 8 cut(s) 1608, 1644, 1663, 1767, 1828, 2000, 2264, 2830
BfaI CTAG 8 cut(s) 261, 869, 897, 1469, 1484, 2154, 2876, 3099
BfmI CTRYAG 1 cut(s) 2341
BfrI CTTAAG 2 cut(s) 1861, 3133
BfuAI ACCTGC 2 cut(s) 833, 3157
BfuI GTATCC 2 cut(s) 481, 1866
BglII AGATCT 5 cut(s) 263, 1520, 1865, 2047, 2395
BisI GCNGC 6 cut(s) 246, 404, 519, 531, 948, 965
BlnI CCTAGG 1 cut(s) 3098
BlsI GCNGC 6 cut(s) 247, 405, 520, 532, 949, 966
Bme1390I CCNGG 4 cut(s) 1059, 1617, 3084, 3474
Bme18I GGWCC 4 cut(s) 1055, 1795, 3413, 3470
BmeT110I CYCGRG 2 cut(s) 783, 2222
BmgT120I GGNCC 5 cut(s) 355, 1055, 1795, 3413, 3470
BmiI GGNNCC 4 cut(s) 390, 407, 746, 3585
BmrFI CCNGG 4 cut(s) 1059, 1617, 3084, 3474
BmrI ACTGGG 2 cut(s) 391, 1860
BmsI GCATC 9 cut(s) 556, 1108, 1225, 1545, 2402, 2543, 2911, 2933, 2987
BmuI ACTGGG 2 cut(s) 391, 1860
BoxI GACNNNNGTC 1 cut(s) 542
BpiI GAAGAC 6 cut(s) 367, 1653, 1766, 2351, 2768, 3032
BplI GAGNNNNNCTC 2 cut(s) 168, 200
BpmI CTGGAG 1 cut(s) 3500
Bpu10I CCTNAGC 1 cut(s) 1245
BpuEI CTTGAG 6 cut(s) 230, 1852, 2057, 2162, 2828, 3467
BpuMI CCSGG 1 cut(s) 3474
Bsa29I ATCGAT 1 cut(s) 62
BsaBI GATNNNNATC 3 cut(s) 1005, 1449, 2400
BsaHI GRCGYC 1 cut(s) 68
BsaI GGTCTC 2 cut(s) 1644, 2000
BsaJI CCNNGG 4 cut(s) 748, 1693, 2089, 3098
Bsc4I CCNNNNNNNGG 4 cut(s) 1496, 1699, 2648, 3223
Bse1I ACTGG 8 cut(s) 151, 386, 1052, 1392, 1855, 2317, 3571, 3597
Bse8I GATNNNNATC 3 cut(s) 1005, 1449, 2400
BseBI CCWGG 3 cut(s) 1059, 1617, 3084
BseCI ATCGAT 1 cut(s) 62
BseDI CCNNGG 4 cut(s) 748, 1693, 2089, 3098
BseGI GGATG 9 cut(s) 130, 202, 571, 937, 1396, 2063, 2417, 2558, 3002
BseJI GATNNNNATC 3 cut(s) 1005, 1449, 2400
BseLI CCNNNNNNNGG 4 cut(s) 1496, 1699, 2648, 3223
BseMII CTCAG 4 cut(s) 288, 531, 1259, 2669
BseNI ACTGG 8 cut(s) 151, 386, 1052, 1392, 1855, 2317, 3571, 3597
BseRI GAGGAG 1 cut(s) 1829
BseXI GCAGC 4 cut(s) 232, 505, 517, 934
BseYI CCCAGC 2 cut(s) 561, 1581
BsgI GTGCAG 1 cut(s) 147
BshFI GGCC 6 cut(s) 232, 357, 867, 1809, 2316, 3596
BshNI GGYRCC 1 cut(s) 405
BshVI ATCGAT 1 cut(s) 62
BsiHKAI GWGCWC 2 cut(s) 429, 3216
BsiHKCI CYCGRG 2 cut(s) 783, 2222
BsiSI CCGG 1 cut(s) 3473
BslFI GGGAC 1 cut(s) 1179
BslI CCNNNNNNNGG 4 cut(s) 1496, 1699, 2648, 3223
BsmAI GTCTC 8 cut(s) 1608, 1644, 1663, 1767, 1828, 2000, 2264, 2830
BsmFI GGGAC 1 cut(s) 1179
BsmI GAATGC 1 cut(s) 684
BsnI GGCC 6 cut(s) 232, 357, 867, 1809, 2316, 3596
Bso31I GGTCTC 2 cut(s) 1644, 2000
BsoBI CYCGRG 2 cut(s) 783, 2222
Bsp1286I GDGCHC 2 cut(s) 429, 3216
Bsp1407I TGTACA 1 cut(s) 877
Bsp19I CCATGG 1 cut(s) 748
BspACI CCGC 7 cut(s) 133, 403, 682, 757, 964, 1262, 2635
BspANI GGCC 6 cut(s) 232, 357, 867, 1809, 2316, 3596
BspCNI CTCAG 4 cut(s) 289, 532, 1258, 2670
BspDI ATCGAT 1 cut(s) 62
BspHI TCATGA 2 cut(s) 688, 1642
BspLI GGNNCC 4 cut(s) 390, 407, 746, 3585
BspMAI CTGCAG 1 cut(s) 2345
BspMI ACCTGC 2 cut(s) 833, 3157
BspPI GGATC 9 cut(s) 383, 396, 739, 752, 1014, 1458, 2578, 3578, 3591
BspT107I GGYRCC 1 cut(s) 405
BspTI CTTAAG 2 cut(s) 1861, 3133
BspTNI GGTCTC 2 cut(s) 1644, 2000
BsrGI TGTACA 1 cut(s) 877
BsrI ACTGG 8 cut(s) 151, 386, 1052, 1392, 1855, 2317, 3571, 3597
BssECI CCNNGG 4 cut(s) 748, 1693, 2089, 3098
BssNAI GTATAC 2 cut(s) 639, 2873
BssNI GRCGYC 1 cut(s) 68
BssSI CACGAG 1 cut(s) 3396
BssT1I CCWWGG 4 cut(s) 748, 1693, 2089, 3098
Bst1107I GTATAC 2 cut(s) 639, 2873
Bst2BI CACGAG 1 cut(s) 3396
Bst2UI CCWGG 3 cut(s) 1059, 1617, 3084
Bst4CI ACNGT 6 cut(s) 1551, 1602, 2459, 3167, 3434, 3560
Bst6I CTCTTC 3 cut(s) 82, 1661, 3295
BstACI GRCGYC 1 cut(s) 68
BstAFI CTTAAG 2 cut(s) 1861, 3133
BstAPI GCANNNNNTGC 1 cut(s) 1564
BstAUI TGTACA 1 cut(s) 877
BstC8I GCNNGC 4 cut(s) 755, 1426, 1481, 2639
BstDSI CCRYGG 1 cut(s) 748
BstEII GGTNACC 1 cut(s) 2740
BstF5I GGATG 9 cut(s) 130, 202, 571, 937, 1396, 2063, 2417, 2558, 3002
BstMAI GTCTC 8 cut(s) 1608, 1644, 1663, 1767, 1828, 2000, 2264, 2830
BstMWI GCNNNNNNNGC 6 cut(s) 527, 759, 1564, 2803, 3191, 3523
BstNI CCWGG 3 cut(s) 1059, 1617, 3084
BstNSI RCATGY 2 cut(s) 518, 757
BstPAI GACNNNNGTC 1 cut(s) 542
BstPI GGTNACC 1 cut(s) 2740
BstSCI CCNGG 4 cut(s) 1057, 1615, 3082, 3472
BstSFI CTRYAG 1 cut(s) 2341
BstV1I GCAGC 4 cut(s) 232, 505, 517, 934
BstV2I GAAGAC 6 cut(s) 367, 1653, 1766, 2351, 2768, 3032
BstX2I RGATCY 9 cut(s) 263, 388, 744, 1520, 1865, 2047, 2395, 2570, 3583
BstXI CCANNNNNNTGG 1 cut(s) 3423
BstYI RGATCY 9 cut(s) 263, 388, 744, 1520, 1865, 2047, 2395, 2570, 3583
BstZ17I GTATAC 2 cut(s) 639, 2873
Bsu15I ATCGAT 1 cut(s) 62
BsuI GTATCC 2 cut(s) 481, 1866
BsuRI GGCC 6 cut(s) 232, 357, 867, 1809, 2316, 3596
BsuTUI ATCGAT 1 cut(s) 62
BtgI CCRYGG 1 cut(s) 748
BtsCI GGATG 9 cut(s) 130, 202, 571, 937, 1396, 2063, 2417, 2558, 3002
BtsIMutI CAGTG 3 cut(s) 1299, 2903, 3590
BveI ACCTGC 2 cut(s) 833, 3157
Cac8I GCNNGC 4 cut(s) 755, 1426, 1481, 2639
CaiI CAGNNNCTG 1 cut(s) 947
CciI TCATGA 2 cut(s) 688, 1642
Cfr13I GGNCC 5 cut(s) 355, 1055, 1795, 3413, 3470
ClaI ATCGAT 1 cut(s) 62
CseI GACGC 2 cut(s) 57, 2065
Csp6I GTAC 4 cut(s) 878, 1508, 2898, 3203
CviQI GTAC 4 cut(s) 878, 1508, 2898, 3203
DraI TTTAAA 1 cut(s) 3460
EaeI YGGCCR 1 cut(s) 3594
Eam1104I CTCTTC 3 cut(s) 82, 1661, 3295
EarI CTCTTC 3 cut(s) 82, 1661, 3295
Ecl136II GAGCTC 1 cut(s) 427
Eco130I CCWWGG 4 cut(s) 748, 1693, 2089, 3098
Eco147I AGGCCT 2 cut(s) 232, 1809
Eco24I GRGCYC 1 cut(s) 429
Eco31I GGTCTC 2 cut(s) 1644, 2000
Eco47I GGWCC 4 cut(s) 1055, 1795, 3413, 3470
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 7 cut(s) 378, 1173, 1685, 1824, 2577, 3044, 3308
Eco88I CYCGRG 2 cut(s) 783, 2222
Eco91I GGTNACC 1 cut(s) 2740
EcoICRI GAGCTC 1 cut(s) 427
EcoO65I GGTNACC 1 cut(s) 2740
EcoRI GAATTC 2 cut(s) 180, 3245
EcoRII CCWGG 3 cut(s) 1057, 1615, 3082
EcoT14I CCWWGG 4 cut(s) 748, 1693, 2089, 3098
EcoT22I ATGCAT 3 cut(s) 344, 1560, 2926
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 4 cut(s) 748, 1693, 2089, 3098
FalI AAGNNNNNCTT 2 cut(s) 873, 905
FaqI GGGAC 1 cut(s) 1179
FauI CCCGC 3 cut(s) 140, 689, 750
FauNDI CATATG 1 cut(s) 710
FbaI TGATCA 2 cut(s) 6, 796
FblI GTMKAC 2 cut(s) 638, 2872
Fnu4HI GCNGC 6 cut(s) 246, 404, 519, 531, 948, 965
FokI GGATG 9 cut(s) 137, 209, 578, 944, 1403, 2070, 2424, 2565, 3009
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 6 cut(s) 246, 404, 519, 531, 948, 965
FspBI CTAG 8 cut(s) 261, 869, 897, 1469, 1484, 2154, 2876, 3099
GluI GCNGC 6 cut(s) 246, 404, 519, 531, 948, 965
GsaI CCCAGC 2 cut(s) 565, 1585
GsuI CTGGAG 1 cut(s) 3500
HaeIII GGCC 6 cut(s) 232, 357, 867, 1809, 2316, 3596
HapII CCGG 1 cut(s) 3473
HgaI GACGC 2 cut(s) 57, 2065
Hin1I GRCGYC 1 cut(s) 68
HincII GTYRAC 4 cut(s) 72, 655, 1014, 1713
HindII GTYRAC 4 cut(s) 72, 655, 1014, 1713
HindIII AAGCTT 3 cut(s) 2105, 2804, 3230
HpaII CCGG 1 cut(s) 3473
HphI GGTGA 6 cut(s) 16, 178, 2708, 2752, 3365, 3413
Hpy99I CGWCG 1 cut(s) 87
HpyCH4III ACNGT 6 cut(s) 1551, 1602, 2459, 3167, 3434, 3560
HpyCH4IV ACGT 3 cut(s) 1362, 1624, 2245
HpyF10VI GCNNNNNNNGC 6 cut(s) 527, 759, 1564, 2803, 3191, 3523
HpySE526I ACGT 3 cut(s) 1362, 1624, 2245
Hsp92I GRCGYC 1 cut(s) 68
Ksp22I TGATCA 2 cut(s) 6, 796
LmnI GCTCC 3 cut(s) 466, 944, 2702
Lsp1109I GCAGC 4 cut(s) 232, 505, 517, 934
LweI GCATC 9 cut(s) 556, 1108, 1225, 1545, 2402, 2543, 2911, 2933, 2987
MaeI CTAG 8 cut(s) 261, 869, 897, 1469, 1484, 2154, 2876, 3099
MaeII ACGT 3 cut(s) 1362, 1624, 2245
MaeIII GTNAC 3 cut(s) 1456, 2723, 2740
MfeI CAATTG 3 cut(s) 249, 2298, 2968
MflI RGATCY 9 cut(s) 263, 388, 744, 1520, 1865, 2047, 2395, 2570, 3583
MhlI GDGCHC 2 cut(s) 429, 3216
MlsI TGGCCA 1 cut(s) 3596
MluNI TGGCCA 1 cut(s) 3596
MlyI GAGTC 6 cut(s) 44, 251, 308, 1830, 2155, 3507
MmeI TCCRAC 3 cut(s) 1563, 2212, 2220
Mox20I TGGCCA 1 cut(s) 3596
Mph1103I ATGCAT 3 cut(s) 344, 1560, 2926
MroXI GAANNNNTTC 7 cut(s) 676, 888, 1158, 1590, 1684, 3029, 3066
MscI TGGCCA 1 cut(s) 3596
MslI CAYNNNNRTG 5 cut(s) 14, 191, 671, 1703, 3240
Msp20I TGGCCA 1 cut(s) 3596
MspCI CTTAAG 2 cut(s) 1861, 3133
MspI CCGG 1 cut(s) 3473
MspR9I CCNGG 4 cut(s) 1059, 1617, 3084, 3474
MunI CAATTG 3 cut(s) 249, 2298, 2968
Mva1269I GAATGC 1 cut(s) 684
MvaI CCWGG 3 cut(s) 1059, 1617, 3084
MwoI GCNNNNNNNGC 6 cut(s) 527, 759, 1564, 2803, 3191, 3523
NciI CCSGG 1 cut(s) 3474
NcoI CCATGG 1 cut(s) 748
NdeI CATATG 1 cut(s) 710
NlaIV GGNNCC 4 cut(s) 390, 407, 746, 3585
NmuCI GTSAC 2 cut(s) 1456, 2740
NsiI ATGCAT 3 cut(s) 344, 1560, 2926
NspI RCATGY 2 cut(s) 518, 757
PaeI GCATGC 1 cut(s) 757
PaeR7I CTCGAG 1 cut(s) 783
PagI TCATGA 2 cut(s) 688, 1642
PceI AGGCCT 2 cut(s) 232, 1809
PctI GAATGC 1 cut(s) 684
PdmI GAANNNNTTC 7 cut(s) 676, 888, 1158, 1590, 1684, 3029, 3066
PflFI GACNNNGTC 2 cut(s) 1490, 3474
PflMI CCANNNNNTGG 2 cut(s) 1699, 3223
PfoI TCCNGGA 2 cut(s) 1057, 3082
PkrI GCNGC 6 cut(s) 247, 405, 520, 532, 949, 966
PleI GAGTC 6 cut(s) 43, 251, 307, 1830, 2154, 3506
PpsI GAGTC 6 cut(s) 43, 251, 307, 1830, 2154, 3506
PshAI GACNNNNGTC 1 cut(s) 542
PshBI ATTAAT 1 cut(s) 2186
Psp124BI GAGCTC 1 cut(s) 429
Psp1406I AACGTT 1 cut(s) 2245
Psp6I CCWGG 3 cut(s) 1057, 1615, 3082
PspEI GGTNACC 1 cut(s) 2740
PspFI CCCAGC 2 cut(s) 561, 1581
PspGI CCWGG 3 cut(s) 1057, 1615, 3082
PspN4I GGNNCC 4 cut(s) 390, 407, 746, 3585
PspPI GGNCC 5 cut(s) 355, 1055, 1795, 3413, 3470
PspXI VCTCGAGB 1 cut(s) 783
PstI CTGCAG 1 cut(s) 2345
PstNI CAGNNNCTG 1 cut(s) 947
PsuI RGATCY 9 cut(s) 263, 388, 744, 1520, 1865, 2047, 2395, 2570, 3583
PsyI GACNNNGTC 2 cut(s) 1490, 3474
RsaI GTAC 4 cut(s) 879, 1509, 2899, 3204
RsaNI GTAC 4 cut(s) 878, 1508, 2898, 3203
RseI CAYNNNNRTG 5 cut(s) 14, 191, 671, 1703, 3240
SacI GAGCTC 1 cut(s) 429
SatI GCNGC 6 cut(s) 246, 404, 519, 531, 948, 965
Sau96I GGNCC 5 cut(s) 355, 1055, 1795, 3413, 3470
SchI GAGTC 6 cut(s) 44, 251, 308, 1830, 2155, 3507
ScrFI CCNGG 4 cut(s) 1059, 1617, 3084, 3474
SduI GDGCHC 2 cut(s) 429, 3216
SfaNI GCATC 9 cut(s) 556, 1108, 1225, 1545, 2402, 2543, 2911, 2933, 2987
SfcI CTRYAG 1 cut(s) 2341
Sfr274I CTCGAG 1 cut(s) 783
SinI GGWCC 4 cut(s) 1055, 1795, 3413, 3470
SlaI CTCGAG 1 cut(s) 783
SmiMI CAYNNNNRTG 5 cut(s) 14, 191, 671, 1703, 3240
SmlI CTYRAG 9 cut(s) 209, 783, 1831, 1861, 2036, 2141, 2807, 3133, 3446
SmoI CTYRAG 9 cut(s) 209, 783, 1831, 1861, 2036, 2141, 2807, 3133, 3446
SphI GCATGC 1 cut(s) 757
SseBI AGGCCT 2 cut(s) 232, 1809
SsiI CCGC 7 cut(s) 133, 403, 682, 757, 964, 1262, 2635
SspI AATATT 1 cut(s) 1410
SspMI CTAG 8 cut(s) 261, 869, 897, 1469, 1484, 2154, 2876, 3099
SstI GAGCTC 1 cut(s) 429
StuI AGGCCT 2 cut(s) 232, 1809
StyD4I CCNGG 4 cut(s) 1057, 1615, 3082, 3472
StyI CCWWGG 4 cut(s) 748, 1693, 2089, 3098
TaaI ACNGT 6 cut(s) 1551, 1602, 2459, 3167, 3434, 3560
TaiI ACGT 3 cut(s) 1365, 1627, 2248
TaqI TCGA 5 cut(s) 62, 784, 1553, 1842, 3294
TaqII GACCGA 1 cut(s) 834
TatI WGTACW 4 cut(s) 877, 1507, 2897, 3202
TauI GCSGC 2 cut(s) 406, 967
TscAI CASTG 3 cut(s) 1306, 2910, 3597
TseFI GTSAC 2 cut(s) 1456, 2740
TseI GCWGC 4 cut(s) 245, 518, 530, 947
Tsp45I GTSAC 2 cut(s) 1456, 2740
TspGWI ACGGA 4 cut(s) 4, 622, 1245, 3491
TspRI CASTG 3 cut(s) 1306, 2910, 3597
Tth111I GACNNNGTC 2 cut(s) 1490, 3474
Van91I CCANNNNNTGG 2 cut(s) 1699, 3223
Vha464I CTTAAG 2 cut(s) 1861, 3133
VpaK11BI GGWCC 4 cut(s) 1055, 1795, 3413, 3470
VspI ATTAAT 1 cut(s) 2186
XbaI TCTAGA 2 cut(s) 260, 896
XceI RCATGY 2 cut(s) 518, 757
XcmI CCANNNNNNNNNTGG 2 cut(s) 2087, 3119
XhoI CTCGAG 1 cut(s) 783
XmaJI CCTAGG 1 cut(s) 3098
XmiI GTMKAC 2 cut(s) 638, 2872
XmnI GAANNNNTTC 7 cut(s) 676, 888, 1158, 1590, 1684, 3029, 3066
XspI CTAG 8 cut(s) 261, 869, 897, 1469, 1484, 2154, 2876, 3099
Zsp2I ATGCAT 3 cut(s) 344, 1560, 2926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.