Rw6G003350

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000105
Physical Location & Seq
Forward (+)
5323012 .. 5327795
4784 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G003350.1

Sequence Viewer

Length: 2826 bp
ATGGCGGCAACCTCTTCTCTCCGTCGATGGAAGTACGATGTGTTTCTAAGTTTCAGAGGGCCAGATACGCGCAAGGGGATTACCTCCGAGCTGTATCATAGACTAGAACGGAGAGGAATTAAAACCTTTATGGATGACCCCGATCTTCAGGTAGGAGATTCCGTTAGCCCTGAACTTTTAGCTGCAATTGAGGAGTCAAGATTTGCCATTGTCATGCTTTCACCCACCTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGACTATTCAATGCATGAAAGAGACGGGGCTACGAGTCATGCCCGTCTTCTACAACGTGGAGCCTTGCGATGTGCGGCACCAAAGGGGAAGTTTTGCGCTCAAAAGGAAACCCCGACACCAAGCAGACGTGGAAGTAAGGGAACATGAAGAAGCTTATGGCAAGACTGAGGATAAATTAAAGGCGTGGAGAGCTGCTTTGAAAGAAGTAGCCGATCTTTCTGGGTGGGATTCAAACAAGTTTAGAACTGATAGAGAACTTGTTGAGGCAATTGTTGGGACAATTGAGAATAAGGTGGTGTATAAATCTTCAAGTGTTGACAAGGGCTTATTAGGAATGGATTTCCGTGTTGATGATTTATTACATAATTACATATATCAAGAGTTGGATGAGGTACGTATTATAGGGATACATGGGATGCGAGGCGTAGGTAAGACAACGTTTGCTCGGGCTTTTTATGATGACTTTTGTCAGGATTTTGAGCATAGATGCTTTCTTCCCAATGTTAGAGAAAGGTTTAAAGGGGGTGGCGTAGTTTCTTTACAAAAGAAGCTTCTTTCCAGCATCTTGATGGACAAAATTGAATACATAGAGGATGAATATGCAGGAGCTGCTATGATTCAAAGTAGTTTATGTAAAACAAAGGTGCTTGCTGTTATTGATGATGTGGATCAGTTGGCACAATTAGAAAAATTGGCTGGAAGTCGCGACTGGTTCGGTCCAGGGAGCAGAATTATCATAACCACCACGGATAATCACCTGTTAGAGGCACATGGTGTTGATGCTACATACAACGCTACTAGCTTAAGTCCTGATGAAGCTCTTGAACTCTTGAGTTTAAAGGCTTTCAAGAAAAGCTCCCCACCAGAAAATTATTCGGACCTGTGTGACGATATTTTAGGGTATGCTCAGGGGCTGCCGTTAGCTCTTGTGGTTATAGGTTCTTTTCTATGTGGTAGAAGCACCAATGAATGGGCAAGTGCAATTGACAGGCTAAAAAATACACCAAATAGAGGAATTATGGATGTGCTTCAGATTAGTTTTGATGGACTAGAGGACATAGACAAAGAAATTTTCCTACATATTGCTTGTTTTTACAAGCGGAAAGAGAAGGATCGTGTGACACAAATACTAGACTATTGTGAGCTGAACCCTGTCATTGGATTAAGAATTCTTGCTGATAAATCTCTCATTACTATTTCCAGCAACAAACTGTCAATGCATGATTTGCTACAAGAACTGGGCTGGGAAATAGTTCGTCGACAGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGAAATCCACAACATGTTGAAGAAAAATAAGGGAACAGAAGCAATCCAAGGCATGGTAATGGAGTTGCCTGAATTAGAAGCGGCTAATTGGAATCCAGAAGCCTTCTCAAATATTTCTCAACTTAGTCTTCTCCATATTCATAATGTGGACCTTCCCAATGGCCTCACTTGTCTTCCTAGTTCCTTGAGACTCCTGGAATGGCCTGGGTGTCCGTTAAGATCTCTCCCACGAAAGTTCGAACCAGATGAACTTATTGAACTTAACTTGTGTCACAGCAATATTGAAAATCTTTGGAAGGGAACAAAGAATTTTAAGAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGCGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTAAGAGTTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTACTGTCCTGAATCTTAAAGACTGCAAAAGTCTTGAGAATCTGCCAGATAGAATTGAAATAGAATCTCTTGAAAAATTTATTCTTTCTAACTGCTCAAAAATTAAAAAGATTCCCGAGTTTGATGGAAATATGGAGTGTTTGTCTGTGCTTCATCTCGACGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGGACGCCTGAGTAGCCTTGTGTCATTGAATCTAAGTAACTGCAAAAATCTTGTTTGTCTTCCAAGCACCATCAATAATTTGAAGTTTGTTGAAAATCTTGATCTTTCTGGATGCATGAAACTTGGCAAACATCAGGAAAGCGTGGGGGAGATGGACTGTTATGAGGAAAATGATTTGCATAGTGGGTCTGCAATAGAAATGTCATCTACCCATGATCTCATAAAGTATGTGAGAGGTTCAATCATTCATGGATGCGAAGTAGTTTGGGGAGCTCTAAATAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAACACAGAGCCCATGAGTTTCTACTTGCCTATATCGGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGATCATTTGCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTATGTGGAAACAATTTTGTTCGTCTTCCTTCAGGCATTAGATTGCTTTCTAAGCTTGAGAACTTTAACGTGCAGAATTGCAGGAGACTTGAAGAGTTGTCAGACCTTCCATCAAATAGTTGTATGAAATTATGTGGCTCCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

941

Amino Acids

106.05

Weight (kDa)

5.75

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 193 2.1e-47 TIR domain
TIR_2 PF13676 14 - 113 3.4e-17 TIR domain
NB-ARC PF00931 217 - 374 5.3e-17 NB-ARC domain
WHD_ROQ1 PF23282 442 - 511 8.8e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 609 - 627 4.6e-06 Leucine Rich Repeat
LRR_14 PF23598 671 - 781 2.2e-10 Leucine-rich repeat region
LRR_13 PF23286 700 - 785 1.9e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 2 cut(s) 1236, 1627
AccI GTMKAC 1 cut(s) 1525
AccII CGCG 2 cut(s) 70, 972
AciI CCGC 4 cut(s) 5, 340, 1366, 1655
AclI AACGTT 1 cut(s) 704
AclWI GGATC 3 cut(s) 942, 1386, 2638
AcsI RAATTY 5 cut(s) 1335, 1434, 1882, 2111, 2645
AcuI CTGAAG 3 cut(s) 131, 1280, 2694
AcyI GRCGYC 1 cut(s) 2233
AdeI CACNNNGTG 1 cut(s) 1040
AfaI GTAC 2 cut(s) 35, 660
AfiI CCNNNNNNNGG 6 cut(s) 1030, 1236, 1277, 1424, 1627, 2576
AflII CTTAAG 1 cut(s) 1069
AflIII ACRYGT 1 cut(s) 1587
AjiI CACGTC 1 cut(s) 394
AjnI CCWGG 4 cut(s) 985, 1543, 1767, 1777
Alw21I GWGCWC 1 cut(s) 2503
Alw26I GTCTC 7 cut(s) 281, 1536, 1572, 1756, 1928, 2192, 2758
AlwI GGATC 3 cut(s) 942, 1386, 2638
AlwNI CAGNNNCTG 2 cut(s) 875, 1180
Ama87I CYCGRG 2 cut(s) 711, 2150
AoxI GGCC 3 cut(s) 59, 1735, 1775
ApeKI GCWGC 4 cut(s) 182, 458, 875, 1180
ApoI RAATTY 5 cut(s) 1335, 1434, 1882, 2111, 2645
Asp700I GAANNNNTTC 1 cut(s) 1518
AspLEI GCGC 2 cut(s) 72, 364
AspS9I GGNCC 4 cut(s) 59, 983, 1144, 1723
AsuHPI GGTGA 4 cut(s) 213, 1013, 2636, 2680
AsuII TTCGAA 1 cut(s) 1812
AvaI CYCGRG 2 cut(s) 711, 2150
AvaII GGWCC 3 cut(s) 983, 1144, 1723
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 2 cut(s) 2503, 2553
BbsI GAAGAC 5 cut(s) 304, 1694, 1739, 2279, 2696
Bbv12I GWGCWC 1 cut(s) 2503
BbvI GCAGC 4 cut(s) 169, 445, 862, 1167
BccI CCATC 8 cut(s) 21, 829, 1304, 2016, 2153, 2306, 2374, 2797
BceAI ACGGC 1 cut(s) 1168
BcgI CGANNNNNNTGC 2 cut(s) 2183, 2217
BciT130I CCWGG 4 cut(s) 987, 1545, 1769, 1779
BciVI GTATCC 1 cut(s) 666
BcoDI GTCTC 7 cut(s) 281, 1536, 1572, 1756, 1928, 2192, 2758
BfaI CTAG 5 cut(s) 104, 1065, 1316, 1397, 1752
BfrI CTTAAG 1 cut(s) 1069
BfuI GTATCC 1 cut(s) 666
BglII AGATCT 2 cut(s) 1793, 1975
BisI GCNGC 7 cut(s) 6, 183, 341, 459, 876, 1181, 1656
BlsI GCNGC 7 cut(s) 7, 184, 342, 460, 877, 1182, 1657
Bme1390I CCNGG 4 cut(s) 987, 1545, 1769, 1779
Bme18I GGWCC 3 cut(s) 983, 1144, 1723
BmeT110I CYCGRG 2 cut(s) 711, 2150
BmgBI CACGTC 1 cut(s) 394
BmgT120I GGNCC 4 cut(s) 59, 983, 1144, 1723
BmiI GGNNCC 3 cut(s) 327, 344, 2818
BmrFI CCNGG 4 cut(s) 987, 1545, 1769, 1779
BmrI ACTGGG 1 cut(s) 1514
BmsI GCATC 6 cut(s) 672, 743, 837, 1036, 2330, 2471
BmuI ACTGGG 1 cut(s) 1514
BoxI GACNNNNGTC 1 cut(s) 732
BpiI GAAGAC 5 cut(s) 304, 1694, 1739, 2279, 2696
Bpu10I CCTNAGC 1 cut(s) 1173
Bpu14I TTCGAA 1 cut(s) 1812
BpuEI CTTGAG 5 cut(s) 1117, 1780, 1985, 2090, 2756
BsaAI YACGTR 1 cut(s) 662
BsaBI GATNNNNATC 1 cut(s) 933
BsaHI GRCGYC 1 cut(s) 2233
BsaI GGTCTC 2 cut(s) 1572, 1928
BsaJI CCNNGG 5 cut(s) 986, 1011, 1621, 1778, 2017
BsaXI ACNNNNNCTCC 4 cut(s) 147, 177, 2162, 2192
Bsc4I CCNNNNNNNGG 6 cut(s) 1030, 1236, 1277, 1424, 1627, 2576
Bse1I ACTGG 2 cut(s) 980, 1509
Bse8I GATNNNNATC 1 cut(s) 933
BseBI CCWGG 4 cut(s) 987, 1545, 1769, 1779
BseDI CCNNGG 5 cut(s) 986, 1011, 1621, 1778, 2017
BseGI GGATG 9 cut(s) 139, 259, 658, 687, 865, 1294, 1991, 2345, 2486
BseJI GATNNNNATC 1 cut(s) 933
BseLI CCNNNNNNNGG 6 cut(s) 1030, 1236, 1277, 1424, 1627, 2576
BseMII CTCAG 4 cut(s) 423, 1187, 2228, 2597
BseNI ACTGG 2 cut(s) 980, 1509
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 4 cut(s) 169, 445, 862, 1167
BseYI CCCAGC 1 cut(s) 1509
BsgI GTGCAG 1 cut(s) 2771
Bsh1236I CGCG 2 cut(s) 70, 972
BshFI GGCC 3 cut(s) 61, 1737, 1777
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 2503
BsiHKCI CYCGRG 2 cut(s) 711, 2150
BslFI GGGAC 1 cut(s) 556
BslI CCNNNNNNNGG 6 cut(s) 1030, 1236, 1277, 1424, 1627, 2576
BsmAI GTCTC 7 cut(s) 281, 1536, 1572, 1756, 1928, 2192, 2758
BsmBI CGTCTC 1 cut(s) 281
BsmFI GGGAC 1 cut(s) 556
BsnI GGCC 3 cut(s) 61, 1737, 1777
Bso31I GGTCTC 2 cut(s) 1572, 1928
BsoBI CYCGRG 2 cut(s) 711, 2150
Bsp119I TTCGAA 1 cut(s) 1812
Bsp1286I GDGCHC 2 cut(s) 2503, 2553
Bsp143I GATC 9 cut(s) 142, 478, 934, 1378, 1793, 1975, 2329, 2443, 2630
Bsp68I TCGCGA 1 cut(s) 972
BspACI CCGC 4 cut(s) 5, 340, 1366, 1655
BspANI GGCC 3 cut(s) 61, 1737, 1777
BspCNI CTCAG 4 cut(s) 424, 1186, 2229, 2598
BspFNI CGCG 2 cut(s) 70, 972
BspHI TCATGA 1 cut(s) 1570
BspLI GGNNCC 3 cut(s) 327, 344, 2818
BspPI GGATC 3 cut(s) 942, 1386, 2638
BspT104I TTCGAA 1 cut(s) 1812
BspT107I GGYRCC 1 cut(s) 342
BspTI CTTAAG 1 cut(s) 1069
BspTNI GGTCTC 2 cut(s) 1572, 1928
BsrI ACTGG 2 cut(s) 980, 1509
BssECI CCNNGG 5 cut(s) 986, 1011, 1621, 1778, 2017
BssMI GATC 9 cut(s) 142, 478, 934, 1378, 1793, 1975, 2329, 2443, 2630
BssNI GRCGYC 1 cut(s) 2233
BssT1I CCWWGG 2 cut(s) 1621, 2017
Bst2UI CCWGG 4 cut(s) 987, 1545, 1769, 1779
Bst4CI ACNGT 4 cut(s) 1479, 1530, 2041, 2387
Bst6I CTCTTC 2 cut(s) 19, 2766
BstACI GRCGYC 1 cut(s) 2233
BstAFI CTTAAG 1 cut(s) 1069
BstAPI GCANNNNNTGC 2 cut(s) 875, 1492
BstBAI YACGTR 1 cut(s) 662
BstBI TTCGAA 1 cut(s) 1812
BstC8I GCNNGC 2 cut(s) 915, 1951
BstDEI CTNAG 8 cut(s) 47, 432, 1173, 1697, 2237, 2261, 2606, 2730
BstDSI CCRYGG 1 cut(s) 1011
BstEII GGTNACC 1 cut(s) 2668
BstENI CCTNNNNNAGG 1 cut(s) 1028
BstF5I GGATG 9 cut(s) 139, 259, 658, 687, 865, 1294, 1991, 2345, 2486
BstFNI CGCG 2 cut(s) 70, 972
BstHHI GCGC 2 cut(s) 72, 364
BstKTI GATC 9 cut(s) 145, 481, 937, 1381, 1796, 1978, 2332, 2446, 2633
BstMAI GTCTC 7 cut(s) 281, 1536, 1572, 1756, 1928, 2192, 2758
BstMBI GATC 9 cut(s) 142, 478, 934, 1378, 1793, 1975, 2329, 2443, 2630
BstMWI GCNNNNNNNGC 6 cut(s) 67, 455, 875, 1492, 2241, 2731
BstNI CCWGG 4 cut(s) 987, 1545, 1769, 1779
BstNSI RCATGY 1 cut(s) 1591
BstPAI GACNNNNGTC 1 cut(s) 732
BstPI GGTNACC 1 cut(s) 2668
BstSCI CCNGG 4 cut(s) 985, 1543, 1767, 1777
BstSNI TACGTA 1 cut(s) 662
BstUI CGCG 2 cut(s) 70, 972
BstV1I GCAGC 4 cut(s) 169, 445, 862, 1167
BstV2I GAAGAC 5 cut(s) 304, 1694, 1739, 2279, 2696
BstX2I RGATCY 2 cut(s) 1793, 1975
BstYI RGATCY 2 cut(s) 1793, 1975
BsuI GTATCC 1 cut(s) 666
BsuRI GGCC 3 cut(s) 61, 1737, 1777
BtgI CCRYGG 1 cut(s) 1011
BtgZI GCGATG 1 cut(s) 348
BtrI CACGTC 1 cut(s) 394
BtsCI GGATG 9 cut(s) 139, 259, 658, 687, 865, 1294, 1991, 2345, 2486
BtuMI TCGCGA 1 cut(s) 972
Cac8I GCNNGC 2 cut(s) 915, 1951
CaiI CAGNNNCTG 2 cut(s) 875, 1180
CciI TCATGA 1 cut(s) 1570
CfoI GCGC 2 cut(s) 72, 364
Cfr13I GGNCC 4 cut(s) 59, 983, 1144, 1723
CseI GACGC 1 cut(s) 2241
Csp6I GTAC 2 cut(s) 34, 659
CviQI GTAC 2 cut(s) 34, 659
DdeI CTNAG 8 cut(s) 47, 432, 1173, 1697, 2237, 2261, 2606, 2730
DpnI GATC 9 cut(s) 144, 480, 936, 1380, 1795, 1977, 2331, 2445, 2632
DpnII GATC 9 cut(s) 142, 478, 934, 1378, 1793, 1975, 2329, 2443, 2630
DraI TTTAAA 2 cut(s) 784, 1104
DraIII CACNNNGTG 1 cut(s) 1040
Eam1104I CTCTTC 2 cut(s) 19, 2766
EarI CTCTTC 2 cut(s) 19, 2766
Ecl136II GAGCTC 1 cut(s) 2501
Eco105I TACGTA 1 cut(s) 662
Eco130I CCWWGG 2 cut(s) 1621, 2017
Eco24I GRGCYC 2 cut(s) 2503, 2553
Eco31I GGTCTC 2 cut(s) 1572, 1928
Eco47I GGWCC 3 cut(s) 983, 1144, 1723
Eco53kI GAGCTC 1 cut(s) 2501
Eco57I CTGAAG 3 cut(s) 131, 1280, 2694
Eco88I CYCGRG 2 cut(s) 711, 2150
Eco91I GGTNACC 1 cut(s) 2668
EcoICRI GAGCTC 1 cut(s) 2501
EcoNI CCTNNNNNAGG 1 cut(s) 1028
EcoO65I GGTNACC 1 cut(s) 2668
EcoRI GAATTC 1 cut(s) 1434
EcoRII CCWGG 4 cut(s) 985, 1543, 1767, 1777
EcoT14I CCWWGG 2 cut(s) 1621, 2017
EcoT22I ATGCAT 3 cut(s) 281, 1488, 2345
EcoT38I GRGCYC 2 cut(s) 2503, 2553
ErhI CCWWGG 2 cut(s) 1621, 2017
Esp3I CGTCTC 1 cut(s) 281
FaqI GGGAC 1 cut(s) 556
FblI GTMKAC 1 cut(s) 1525
Fnu4HI GCNGC 7 cut(s) 6, 183, 341, 459, 876, 1181, 1656
FokI GGATG 9 cut(s) 146, 266, 665, 694, 872, 1301, 1998, 2352, 2493
FriOI GRGCYC 2 cut(s) 2503, 2553
Fsp4HI GCNGC 7 cut(s) 6, 183, 341, 459, 876, 1181, 1656
FspBI CTAG 5 cut(s) 104, 1065, 1316, 1397, 1752
GlaI GCGC 2 cut(s) 71, 363
GluI GCNGC 7 cut(s) 6, 183, 341, 459, 876, 1181, 1656
GsaI CCCAGC 1 cut(s) 1513
HaeIII GGCC 3 cut(s) 61, 1737, 1777
HgaI GACGC 1 cut(s) 2241
HhaI GCGC 2 cut(s) 72, 364
Hin1I GRCGYC 1 cut(s) 2233
Hin6I GCGC 2 cut(s) 70, 362
HinP1I GCGC 2 cut(s) 70, 362
HincII GTYRAC 2 cut(s) 583, 1526
HindII GTYRAC 2 cut(s) 583, 1526
HindIII AAGCTT 4 cut(s) 417, 815, 2033, 2732
HphI GGTGA 4 cut(s) 213, 1013, 2636, 2680
Hpy166II GTNNAC 4 cut(s) 583, 1526, 1723, 2542
Hpy188I TCNGA 7 cut(s) 56, 88, 1144, 1299, 1959, 2601, 2782
Hpy8I GTNNAC 4 cut(s) 583, 1526, 1723, 2542
Hpy99I CGWCG 3 cut(s) 27, 1527, 2198
HpyAV CCTTC 9 cut(s) 1369, 1687, 1736, 1864, 1976, 2529, 2621, 2718, 2795
HpyCH4III ACNGT 4 cut(s) 1479, 1530, 2041, 2387
HpyCH4IV ACGT 6 cut(s) 321, 393, 661, 704, 1552, 2748
HpyF10VI GCNNNNNNNGC 6 cut(s) 67, 455, 875, 1492, 2241, 2731
HpyF3I CTNAG 8 cut(s) 47, 432, 1173, 1697, 2237, 2261, 2606, 2730
HpySE526I ACGT 6 cut(s) 321, 393, 661, 704, 1552, 2748
Hsp92I GRCGYC 1 cut(s) 2233
HspAI GCGC 2 cut(s) 70, 362
Kzo9I GATC 9 cut(s) 142, 478, 934, 1378, 1793, 1975, 2329, 2443, 2630
LmnI GCTCC 6 cut(s) 325, 872, 990, 1127, 2498, 2822
Lsp1109I GCAGC 4 cut(s) 169, 445, 862, 1167
LweI GCATC 6 cut(s) 672, 743, 837, 1036, 2330, 2471
MaeI CTAG 5 cut(s) 104, 1065, 1316, 1397, 1752
MaeII ACGT 6 cut(s) 321, 393, 661, 704, 1552, 2748
MaeIII GTNAC 6 cut(s) 1151, 1384, 1844, 2264, 2651, 2668
MalI GATC 9 cut(s) 144, 480, 936, 1380, 1795, 1977, 2331, 2445, 2632
MboI GATC 9 cut(s) 142, 478, 934, 1378, 1793, 1975, 2329, 2443, 2630
MfeI CAATTG 5 cut(s) 186, 534, 546, 1248, 2226
MflI RGATCY 2 cut(s) 1793, 1975
MhlI GDGCHC 2 cut(s) 2503, 2553
MlyI GAGTC 3 cut(s) 203, 309, 1758
MmeI TCCRAC 1 cut(s) 630
Mph1103I ATGCAT 3 cut(s) 281, 1488, 2345
MroXI GAANNNNTTC 1 cut(s) 1518
MslI CAYNNNNRTG 3 cut(s) 212, 833, 1631
MspCI CTTAAG 1 cut(s) 1069
MspR9I CCNGG 4 cut(s) 987, 1545, 1769, 1779
MunI CAATTG 5 cut(s) 186, 534, 546, 1248, 2226
MvaI CCWGG 4 cut(s) 987, 1545, 1769, 1779
MvnI CGCG 2 cut(s) 70, 972
MwoI GCNNNNNNNGC 6 cut(s) 67, 455, 875, 1492, 2241, 2731
NdeII GATC 9 cut(s) 142, 478, 934, 1378, 1793, 1975, 2329, 2443, 2630
NlaIV GGNNCC 3 cut(s) 327, 344, 2818
NmuCI GTSAC 4 cut(s) 1151, 1384, 1844, 2668
NruI TCGCGA 1 cut(s) 972
NsiI ATGCAT 3 cut(s) 281, 1488, 2345
NspI RCATGY 1 cut(s) 1591
NspV TTCGAA 1 cut(s) 1812
PagI TCATGA 1 cut(s) 1570
PciI ACATGT 1 cut(s) 1587
PdmI GAANNNNTTC 1 cut(s) 1518
PflMI CCANNNNNTGG 2 cut(s) 1236, 1627
PfoI TCCNGGA 1 cut(s) 1767
PkrI GCNGC 7 cut(s) 7, 184, 342, 460, 877, 1182, 1657
PleI GAGTC 3 cut(s) 202, 308, 1758
PpsI GAGTC 3 cut(s) 202, 308, 1758
Ppu21I YACGTR 1 cut(s) 662
PscI ACATGT 1 cut(s) 1587
PshAI GACNNNNGTC 1 cut(s) 732
Psp124BI GAGCTC 1 cut(s) 2503
Psp1406I AACGTT 1 cut(s) 704
Psp6I CCWGG 4 cut(s) 985, 1543, 1767, 1777
PspEI GGTNACC 1 cut(s) 2668
PspFI CCCAGC 1 cut(s) 1509
PspGI CCWGG 4 cut(s) 985, 1543, 1767, 1777
PspN4I GGNNCC 3 cut(s) 327, 344, 2818
PspPI GGNCC 4 cut(s) 59, 983, 1144, 1723
PstNI CAGNNNCTG 2 cut(s) 875, 1180
PsuI RGATCY 2 cut(s) 1793, 1975
RruI TCGCGA 1 cut(s) 972
RsaI GTAC 2 cut(s) 35, 660
RsaNI GTAC 2 cut(s) 34, 659
RseI CAYNNNNRTG 3 cut(s) 212, 833, 1631
SacI GAGCTC 1 cut(s) 2503
SalI GTCGAC 1 cut(s) 1524
SatI GCNGC 7 cut(s) 6, 183, 341, 459, 876, 1181, 1656
Sau3AI GATC 9 cut(s) 142, 478, 934, 1378, 1793, 1975, 2329, 2443, 2630
Sau96I GGNCC 4 cut(s) 59, 983, 1144, 1723
SchI GAGTC 3 cut(s) 203, 309, 1758
ScrFI CCNGG 4 cut(s) 987, 1545, 1769, 1779
SduI GDGCHC 2 cut(s) 2503, 2553
SfaNI GCATC 6 cut(s) 672, 743, 837, 1036, 2330, 2471
SfuI TTCGAA 1 cut(s) 1812
SinI GGWCC 3 cut(s) 983, 1144, 1723
SmiMI CAYNNNNRTG 3 cut(s) 212, 833, 1631
SmlI CTYRAG 6 cut(s) 1069, 1096, 1759, 1964, 2069, 2735
SmoI CTYRAG 6 cut(s) 1069, 1096, 1759, 1964, 2069, 2735
SnaBI TACGTA 1 cut(s) 662
SsiI CCGC 4 cut(s) 5, 340, 1366, 1655
SspI AATATT 2 cut(s) 1687, 1855
SspMI CTAG 5 cut(s) 104, 1065, 1316, 1397, 1752
SstI GAGCTC 1 cut(s) 2503
StyD4I CCNGG 4 cut(s) 985, 1543, 1767, 1777
StyI CCWWGG 2 cut(s) 1621, 2017
TaaI ACNGT 4 cut(s) 1479, 1530, 2041, 2387
TaiI ACGT 6 cut(s) 324, 396, 664, 707, 1555, 2751
TaqI TCGA 4 cut(s) 25, 1525, 1812, 2193
TaqII GACCGA 1 cut(s) 971
TauI GCSGC 3 cut(s) 8, 343, 1658
TseFI GTSAC 4 cut(s) 1151, 1384, 1844, 2668
TseI GCWGC 4 cut(s) 182, 458, 875, 1180
Tsp45I GTSAC 4 cut(s) 1151, 1384, 1844, 2668
TspGWI ACGGA 6 cut(s) 11, 124, 151, 599, 1028, 1776
Van91I CCANNNNNTGG 2 cut(s) 1236, 1627
Vha464I CTTAAG 1 cut(s) 1069
VpaK11BI GGWCC 3 cut(s) 983, 1144, 1723
XagI CCTNNNNNAGG 1 cut(s) 1028
XapI RAATTY 5 cut(s) 1335, 1434, 1882, 2111, 2645
XceI RCATGY 1 cut(s) 1591
XcmI CCANNNNNNNNNTGG 2 cut(s) 832, 2015
XmiI GTMKAC 1 cut(s) 1525
XmnI GAANNNNTTC 1 cut(s) 1518
XspI CTAG 5 cut(s) 104, 1065, 1316, 1397, 1752
Zsp2I ATGCAT 3 cut(s) 281, 1488, 2345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.