Rw6G000830

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000105
Physical Location & Seq
Forward (+)
1295658 .. 1299973
4316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G000830.1

Sequence Viewer

Length: 3378 bp
ATGACTACCCAAGCAGATATTCCTTCTTCTTCTGCTCCTCTCCCTCCGATCCCTAAATACGGTGTCTTTCTCAGTTCCAGCGGAGAAGACACCGATCAAAATTTTACAGATCATCTCTGTTATGCTCTGAACCGGAAGGGGATATTGACATTTAGAGATGGTGAAGAACTCGAGAGGGGAAAAGCAATTGAAGAATCAAGATGTGCAATTGTGGTTCTTTCAGGAAACTACACCAATCCATGGTGTTTGGATGCGATTGCAAAGATCGTTGAATGCAAGGAAGTGATGATGAAACTGGGAGTACTCCCAGTCTTTCTTGATGTGAATCCATCTGATGTACGAAAACAAACAGGGGATCATTTCGGGGAAGCCTTCTCGAAGCATGAAGAGGCCTTTAAACACGAACCGGAGAAGGTGCGCAGGTGGAGACGTGCTCTTACTTCAGTAACCAATCTCTCTGGATGGCTTGTGCAAAATCGTCATGAAGCCAAAGTTATTCAAGAAATGACAGAAGTTATTCTCATAGAATTCTTTCCTAGAATCCCAAGTGTACCTGAGGGATTAGTTGGGATGGATTCTCACCTGAACGAAATGCTGTCGTACTTGGAAGTGGGGCGTCCTGGAGTTCGTACCGTAGGGATTTGGGGGATGGGGGGTATCGGAAAGACAACTATTGCAAAAGCTATTTATGAAAGGATTTATTCTCAGTTTGAAGGTCACGTCTTTCTTGAGAATGTCAGAGAATCAAGTCGTATGCCAGGTGGTAGTATTGGTTTACAAGAGGAACTTCTTTCAAACTTGCTGAGACGTAATGTAAATGTACGTGACATCACTAGGGGAAGAACCGTGATCAGGGAAAGACTACATACTAGAATGGTTCTTATCGTTGTTGATGATGTGGATGAACTAGAACAATTGGAAGTATTATGTGGTGCTGCTAGTTGGTTCGGGTCAGGGAGTAGAATCATCATAACATCAAGAGATGATCATTTGCTGAAAGCATTTAGTGTGGAGAACATATACAAGGTCAATGCATTATCTGATGTTGAGGCTCTTCAGCTGTTTTGTTCAAGAGCCTTCAAGAAGGACCAGGTTGGAGAAGATGTTCTCAAGCTTTCTAAGGAGTTTGTAAAATATGCTGCTGGCCTTCCATTAGCTCTTCAAGTATTGGCTGCATCTCTCCGTGGTAAGGAAAAAACTCTGTGGTCAAGTGCATTGGATAGACTAAAACAGAAACCTGATAGAGAAATTATGAATGTGCTTAAGGTAAGTTATGATGGACTAGATGACTTAGAAAAGGTTATTTTTTTAGACATTGCATGCTTCTTTAAAGGAGAGAACGTAGATTGTGTAGAAAGAATACTAGGGGTCGATTATTTTCCAAACTTTGGTATCAGAGTTCTTCTGGATAGATCTCTAGTAACTGTGGTTGGAAGAAAATTATGGATGCATGACCTGCTGCAACAATTGGGTAAAGCAATTGTTATTCAAGAATGTCTTGAAGAGCCTGGCAATCGTAGCAGGTTGTGGGTTCCGCAAGAAATCATCCATGTACTCGAGAAGAATAAGGGTTCAAGTGCACTGCGAGCGATAGCCTTAGACTTTCCAAAAACAGAAGAGATACACTGCCACTCAGAGGCATTCTCTGTGATGCCCAGACTTGAGTTTCTCCAAATCCGTAATGTGCATATGAAGGAAGGCCCCAGTTATCTTCCTGGGGGATTAGTAGTTCTGGAATGGAGTGGGTATCCTATAGAAAGTCTCCCACAAAATTTCAATCCTAAGCGATTATGTGAACTTAACCTCTGCTACAGCAGCATTGAACAACTTTGGAGTGGAATAAAGAAATTGCCCTATTTGACAGTTATCAATTTAAGCTACTCCTGGAAGTTAATCAGGACCCCAGATTTTACACATGCTCCAGGGCTTGAAAGATTAATTCTTGAAGGTTGTACAAGTTTAGTGGACATCCACCCATCCATTGCTCACCTGGAGAGGCTTATTAGCTTGAATCTTAAAGATTGCAGAAGTCTTGCAAGTCTTCCAAGTAAGCTTGAAACGAAATGCCTTCGAATTCTTGTTCTTTCAGGCTGTTCAAAAGTCAAGAACATTCCAGATTTTGTGGGATCTATGGAAGGCTTGTTAGAACTTTATTTGGATGGGACTGCTATAGAGCAAATACCTATATCAGTTCGACTTCTGACTGGCCTCATTTTATTAAATTTGAGAGATTGCAAAAATCTCAAGAGACTTCCAGGAGACATTGGCAATTTAAAGTCCCTCAAGCGTCTCAATATTTGTGGATGCTCAAAACTTGAAACACTACCAGACAGCTTGGGAAAGATAGACTGCTTGGAAGAGCTTGATGCGGCTGGGGGTAGTTCCATAAGCAATATACCATCGTCTATTTCCCGTTTGGAAAGTCTTGAAGTGTTATCTCTTTGTGGATGTAAAGGGATGTCAGGCAACATGACAAGATATCCACTCAGTTTTCTGTTGCACAAAAGTGATTCAGGTGGCTTGCGTTCTTTAACTGAGCTAAATCTCAGTGACTGTAATCTTCAGGAGGGAAGCATACCGGAAGATTTTGGCTGCTTATTCCCTTTAGTTTCACTAAATCTAAGCAAAAACAACTTTGTTAGTCTTCCAAAAAGCATCAGACAGCTTTCAAAGCTTCGAAACTTGAATTTGGAAAGTTGTAAGACTCTTCAAAAATTGCCAGACCTTTCATCAAGCATAAACTTCAGTTTTGGGGCAGAGGGCGGTCTTTCACAGGAAGGGCTGTCATCATGTTTCAATTTGATTAATTGCTCCAAATTGGTTGTCAATCAACGATGCAACAACATAGCATTGAAAATGCTATCGAGATTTCTTCAGGGAATTCCTTCTGCAGGAAATAGATTTGAAACTATAATTCCTGGCAGTGAAATCTCTGAGCTGTTCAGTGATCAAAGTGTGGAACCAGCAATAAGTATGGAGCTACCTCGACAATGTGACAAGAATAAGTGGATGGGATATGCATTTTGTGCTTGTTTTGTTATCCGTCGAGATCTTCCAGTTCCAGCTAACCGGCTTGGTAAATGGAAGTTAGGAACCCATAGCACTGCAAATGGTCTTCGATGCGAAGTGAAGCCTGAGAATCTGGGTGTGGCTGGCTGGTGTCCTTCCTTTGCCTGCAGCCAAGAATTAGGCCAAATTGAGTCGGAACACCTTTGGCTATCCTTTGTCTCTGGTGATTATTTTGGAACAGCATGGCAAGAAACCTGTCATAACATTGAGTTTACATTCAAAACATTAGGCACTGGTATGGAGGTGAAGAAGTGTGGAGTCCGTCTGATATACGAGCAAGATTTAGATTTGACTGGAATGAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

1125

Amino Acids

125.64

Weight (kDa)

6.54

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 19 - 173 5.8e-36 TIR domain
TIR_2 PF13676 22 - 111 1.3e-07 TIR domain
NB-ARC PF00931 196 - 363 9.1e-20 NB-ARC domain
NACHT PF05729 210 - 356 4.7e-06 NACHT domain
WHD_ROQ1 PF23282 432 - 496 5.8e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 679 - 816 3.1e-14 Leucine-rich repeat region
LRR_13 PF23286 689 - 772 1e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 774 - 909 7.3e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 972 - 1118 3.4e-08 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 411
Acc16I TGCGCA 1 cut(s) 419
Acc36I ACCTGC 3 cut(s) 411, 1464, 1512
AccB7I CCANNNNNTGG 2 cut(s) 240, 1388
AciI CCGC 4 cut(s) 81, 1535, 2369, 2763
AclWI GGATC 3 cut(s) 43, 363, 2134
AcsI RAATTY 7 cut(s) 100, 527, 1771, 2073, 2221, 2686, 2880
AcuI CTGAAG 5 cut(s) 426, 1040, 2546, 2728, 2858
AcyI GRCGYC 1 cut(s) 616
AfaI GTAC 8 cut(s) 303, 339, 552, 602, 631, 822, 1554, 1954
AfiI CCNNNNNNNGG 7 cut(s) 59, 240, 852, 1189, 1388, 1636, 2891
AflII CTTAAG 1 cut(s) 1262
AjiI CACGTC 2 cut(s) 431, 721
AjuI GAANNNNNNNTTGG 1 cut(s) 35
AleI CACNNNNGTG 1 cut(s) 2505
AloI GAACNNNNNNTCC 4 cut(s) 1089, 1121, 1713, 1745
Alw21I GWGCWC 2 cut(s) 436, 1582
Alw26I GTCTC 7 cut(s) 421, 799, 1766, 2242, 2253, 2294, 3232
Alw44I GTGCAC 1 cut(s) 1578
AlwI GGATC 3 cut(s) 43, 363, 2134
AlwNI CAGNNNCTG 1 cut(s) 2553
Ama87I CYCGRG 2 cut(s) 170, 1556
AoxI GGCC 5 cut(s) 390, 1144, 1699, 2206, 3190
ApaLI GTGCAC 1 cut(s) 1578
ApeKI GCWGC 7 cut(s) 935, 1139, 1172, 1459, 1815, 2592, 3177
ApoI RAATTY 7 cut(s) 100, 527, 1771, 2073, 2221, 2686, 2880
ArsI GACNNNNNNTTYG 2 cut(s) 2808, 2840
AseI ATTAAT 2 cut(s) 1937, 2805
Asp700I GAANNNNTTC 4 cut(s) 516, 531, 1104, 2884
AspLEI GCGC 1 cut(s) 420
AspS9I GGNCC 3 cut(s) 1087, 1700, 1899
AsuHPI GGTGA 5 cut(s) 173, 572, 1979, 3245, 3325
AsuII TTCGAA 2 cut(s) 2071, 2677
AvaI CYCGRG 2 cut(s) 170, 1556
AvaII GGWCC 2 cut(s) 1087, 1899
AxyI CCTNAGG 1 cut(s) 555
BaeGI GKGCMC 1 cut(s) 1582
BaeI ACNNNNGTAYC 2 cut(s) 1375, 1408
BbsI GAAGAC 4 cut(s) 93, 2033, 2636, 3107
Bbv12I GWGCWC 2 cut(s) 436, 1582
BbvI GCAGC 7 cut(s) 922, 1126, 1159, 1446, 1827, 2579, 3189
BciVI GTATCC 1 cut(s) 1758
BclI TGATCA 3 cut(s) 849, 985, 2947
BcoDI GTCTC 7 cut(s) 421, 799, 1766, 2242, 2253, 2294, 3232
BfaI CTAG 8 cut(s) 537, 834, 870, 908, 939, 1283, 1364, 1418
BfmI CTRYAG 5 cut(s) 1752, 1810, 2169, 2889, 3175
BfrI CTTAAG 1 cut(s) 1262
BfuAI ACCTGC 3 cut(s) 411, 1464, 1512
BfuI GTATCC 1 cut(s) 1758
BglII AGATCT 2 cut(s) 1412, 3049
BisI GCNGC 8 cut(s) 936, 1140, 1173, 1460, 1816, 2370, 2593, 3178
BlsI GCNGC 8 cut(s) 937, 1141, 1174, 1461, 1817, 2371, 2594, 3179
BmcAI AGTACT 1 cut(s) 303
Bme18I GGWCC 2 cut(s) 1087, 1899
BmeT110I CYCGRG 2 cut(s) 170, 1556
BmgBI CACGTC 2 cut(s) 431, 721
BmgT120I GGNCC 3 cut(s) 1087, 1700, 1899
BmiI GGNNCC 5 cut(s) 1533, 1702, 1901, 2961, 3094
BmrI ACTGGG 3 cut(s) 302, 305, 1698
BmsI GCATC 9 cut(s) 241, 1184, 1437, 1641, 2294, 2356, 2664, 2825, 3110
BmuI ACTGGG 3 cut(s) 302, 305, 1698
BpiI GAAGAC 4 cut(s) 93, 2033, 2636, 3107
BplI GAGNNNNNCTC 4 cut(s) 418, 450, 1628, 1660
BpmI CTGGAG 3 cut(s) 642, 1905, 2012
Bpu10I CCTNAGC 1 cut(s) 1782
Bpu14I TTCGAA 2 cut(s) 2071, 2677
BpuEI CTTGAG 5 cut(s) 749, 1094, 1682, 2228, 2267
BsaAI YACGTR 1 cut(s) 824
BsaBI GATNNNNATC 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 616
BsaJI CCNNGG 4 cut(s) 239, 1183, 1715, 1922
BsaWI WCCGGW 3 cut(s) 132, 406, 2578
BsaXI ACNNNNNCTCC 6 cut(s) 1089, 1119, 1326, 1356, 1903, 1933
Bsc4I CCNNNNNNNGG 7 cut(s) 59, 240, 852, 1189, 1388, 1636, 2891
Bse118I RCCGGY 1 cut(s) 3069
Bse1I ACTGG 7 cut(s) 300, 308, 1704, 2209, 3056, 3307, 3367
Bse21I CCTNAGG 1 cut(s) 555
Bse3DI GCAATG 2 cut(s) 1314, 1980
Bse8I GATNNNNATC 1 cut(s) 324
BseDI CCNNGG 4 cut(s) 239, 1183, 1715, 1922
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 7 cut(s) 59, 240, 852, 1189, 1388, 1636, 2891
BseMI GCAATG 2 cut(s) 1314, 1980
BseNI ACTGG 7 cut(s) 300, 308, 1704, 2209, 3056, 3307, 3367
BseRI GAGGAG 1 cut(s) 27
BseSI GKGCMC 1 cut(s) 1582
BseXI GCAGC 7 cut(s) 922, 1126, 1159, 1446, 1827, 2579, 3189
BseYI CCCAGC 1 cut(s) 2372
BshFI GGCC 5 cut(s) 392, 1146, 1701, 2208, 3192
BsiHKAI GWGCWC 2 cut(s) 436, 1582
BsiHKCI CYCGRG 2 cut(s) 170, 1556
BsiSI CCGG 4 cut(s) 133, 407, 2579, 3070
BslFI GGGAC 2 cut(s) 2176, 2263
BslI CCNNNNNNNGG 7 cut(s) 59, 240, 852, 1189, 1388, 1636, 2891
BsmAI GTCTC 7 cut(s) 421, 799, 1766, 2242, 2253, 2294, 3232
BsmBI CGTCTC 3 cut(s) 421, 799, 2294
BsmFI GGGAC 2 cut(s) 2176, 2263
BsmI GAATGC 2 cut(s) 278, 1640
BsnI GGCC 5 cut(s) 392, 1146, 1701, 2208, 3192
BsoBI CYCGRG 2 cut(s) 170, 1556
Bsp119I TTCGAA 2 cut(s) 2071, 2677
Bsp1286I GDGCHC 2 cut(s) 436, 1582
Bsp1407I TGTACA 1 cut(s) 1952
Bsp19I CCATGG 1 cut(s) 239
BspACI CCGC 4 cut(s) 81, 1535, 2369, 2763
BspANI GGCC 5 cut(s) 392, 1146, 1701, 2208, 3192
BspHI TCATGA 1 cut(s) 481
BspLI GGNNCC 5 cut(s) 1533, 1702, 1901, 2961, 3094
BspMAI CTGCAG 2 cut(s) 2893, 3179
BspMI ACCTGC 3 cut(s) 411, 1464, 1512
BspPI GGATC 3 cut(s) 43, 363, 2134
BspQI GCTCTTC 4 cut(s) 1059, 1164, 1497, 2352
BspT104I TTCGAA 2 cut(s) 2071, 2677
BspTI CTTAAG 1 cut(s) 1262
BsrDI GCAATG 2 cut(s) 1314, 1980
BsrFI RCCGGY 1 cut(s) 3069
BsrGI TGTACA 1 cut(s) 1952
BsrI ACTGG 7 cut(s) 300, 308, 1704, 2209, 3056, 3307, 3367
BssAI RCCGGY 1 cut(s) 3069
BssECI CCNNGG 4 cut(s) 239, 1183, 1715, 1922
BssNI GRCGYC 1 cut(s) 616
BssT1I CCWWGG 1 cut(s) 239
Bst4CI ACNGT 6 cut(s) 62, 634, 847, 1426, 1864, 2555
Bst6I CTCTTC 7 cut(s) 381, 1059, 1164, 1497, 1611, 2352, 2712
BstACI GRCGYC 1 cut(s) 616
BstAFI CTTAAG 1 cut(s) 1262
BstAPI GCANNNNNTGC 2 cut(s) 1456, 3026
BstAUI TGTACA 1 cut(s) 1952
BstBAI YACGTR 1 cut(s) 824
BstBI TTCGAA 2 cut(s) 2071, 2677
BstC8I GCNNGC 6 cut(s) 1144, 1321, 1587, 2522, 3154, 3175
BstDSI CCRYGG 2 cut(s) 239, 1183
BstENI CCTNNNNNAGG 1 cut(s) 2889
BstHHI GCGC 1 cut(s) 420
BstMAI GTCTC 7 cut(s) 421, 799, 1766, 2242, 2253, 2294, 3232
BstMWI GCNNNNNNNGC 6 cut(s) 1456, 1518, 1586, 1815, 2671, 3026
BstNSI RCATGY 2 cut(s) 1323, 1919
BstSFI CTRYAG 5 cut(s) 1752, 1810, 2169, 2889, 3175
BstSLI GKGCMC 1 cut(s) 1582
BstV1I GCAGC 7 cut(s) 922, 1126, 1159, 1446, 1827, 2579, 3189
BstV2I GAAGAC 4 cut(s) 93, 2033, 2636, 3107
BstX2I RGATCY 3 cut(s) 1412, 2126, 3049
BstYI RGATCY 3 cut(s) 1412, 2126, 3049
Bsu36I CCTNAGG 1 cut(s) 555
BsuI GTATCC 1 cut(s) 1758
BsuRI GGCC 5 cut(s) 392, 1146, 1701, 2208, 3192
BtgI CCRYGG 2 cut(s) 239, 1183
BtrI CACGTC 2 cut(s) 431, 721
BtsI GCAGTG 4 cut(s) 1580, 1624, 2929, 3102
BtsIMutI CAGTG 7 cut(s) 1580, 1624, 2554, 2929, 2950, 3102, 3300
BveI ACCTGC 3 cut(s) 411, 1464, 1512
Cac8I GCNNGC 6 cut(s) 1144, 1321, 1587, 2522, 3154, 3175
CaiI CAGNNNCTG 1 cut(s) 2553
CciI TCATGA 1 cut(s) 481
CfoI GCGC 1 cut(s) 420
Cfr10I RCCGGY 1 cut(s) 3069
Cfr13I GGNCC 3 cut(s) 1087, 1700, 1899
CseI GACGC 2 cut(s) 605, 2276
CsiI ACCWGGT 1 cut(s) 1089
Csp6I GTAC 8 cut(s) 302, 338, 551, 601, 630, 821, 1553, 1953
CspCI CAANNNNNGTGG 4 cut(s) 1944, 1979, 2281, 2316
CviQI GTAC 8 cut(s) 302, 338, 551, 601, 630, 821, 1553, 1953
DraI TTTAAA 3 cut(s) 397, 1330, 2274
Eam1104I CTCTTC 7 cut(s) 381, 1059, 1164, 1497, 1611, 2352, 2712
EarI CTCTTC 7 cut(s) 381, 1059, 1164, 1497, 1611, 2352, 2712
Eco130I CCWWGG 1 cut(s) 239
Eco147I AGGCCT 1 cut(s) 392
Eco32I GATATC 1 cut(s) 2480
Eco47I GGWCC 2 cut(s) 1087, 1899
Eco57I CTGAAG 5 cut(s) 426, 1040, 2546, 2728, 2858
Eco81I CCTNAGG 1 cut(s) 555
Eco88I CYCGRG 2 cut(s) 170, 1556
EcoNI CCTNNNNNAGG 1 cut(s) 2889
EcoO109I RGGNCCY 2 cut(s) 1700, 1899
EcoRI GAATTC 3 cut(s) 527, 2073, 2880
EcoRV GATATC 1 cut(s) 2480
EcoT14I CCWWGG 1 cut(s) 239
EcoT22I ATGCAT 3 cut(s) 1036, 1452, 3022
ErhI CCWWGG 1 cut(s) 239
Esp3I CGTCTC 3 cut(s) 421, 799, 2294
FalI AAGNNNNNCTT 4 cut(s) 771, 803, 1482, 1514
FaqI GGGAC 2 cut(s) 2176, 2263
FauNDI CATATG 1 cut(s) 1689
FbaI TGATCA 3 cut(s) 849, 985, 2947
Fnu4HI GCNGC 8 cut(s) 936, 1140, 1173, 1460, 1816, 2370, 2593, 3178
Fsp4HI GCNGC 8 cut(s) 936, 1140, 1173, 1460, 1816, 2370, 2593, 3178
FspBI CTAG 8 cut(s) 537, 834, 870, 908, 939, 1283, 1364, 1418
FspI TGCGCA 1 cut(s) 419
GlaI GCGC 1 cut(s) 419
GluI GCNGC 8 cut(s) 936, 1140, 1173, 1460, 1816, 2370, 2593, 3178
GsaI CCCAGC 1 cut(s) 2376
GsuI CTGGAG 3 cut(s) 642, 1905, 2012
HaeIII GGCC 5 cut(s) 392, 1146, 1701, 2208, 3192
HapII CCGG 4 cut(s) 133, 407, 2579, 3070
HgaI GACGC 2 cut(s) 605, 2276
HhaI GCGC 1 cut(s) 420
Hin1I GRCGYC 1 cut(s) 616
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HindIII AAGCTT 3 cut(s) 1112, 2051, 2672
HpaII CCGG 4 cut(s) 133, 407, 2579, 3070
HphI GGTGA 5 cut(s) 173, 572, 1979, 3245, 3325
Hpy166II GTNNAC 6 cut(s) 551, 776, 1580, 1796, 1966, 3282
Hpy8I GTNNAC 6 cut(s) 551, 776, 1580, 1796, 1966, 3282
Hpy99I CGWCG 1 cut(s) 3048
HpyCH4III ACNGT 6 cut(s) 62, 634, 847, 1426, 1864, 2555
HpyCH4IV ACGT 5 cut(s) 430, 720, 808, 823, 1341
HpyF10VI GCNNNNNNNGC 6 cut(s) 1456, 1518, 1586, 1815, 2671, 3026
HpySE526I ACGT 5 cut(s) 430, 720, 808, 823, 1341
Hsp92I GRCGYC 1 cut(s) 616
HspAI GCGC 1 cut(s) 418
Ksp22I TGATCA 3 cut(s) 849, 985, 2947
LguI GCTCTTC 4 cut(s) 1059, 1164, 1497, 2352
LmnI GCTCC 4 cut(s) 40, 1924, 2816, 2977
Lsp1109I GCAGC 7 cut(s) 922, 1126, 1159, 1446, 1827, 2579, 3189
LweI GCATC 9 cut(s) 241, 1184, 1437, 1641, 2294, 2356, 2664, 2825, 3110
MabI ACCWGGT 1 cut(s) 1089
MaeI CTAG 8 cut(s) 537, 834, 870, 908, 939, 1283, 1364, 1418
MaeII ACGT 5 cut(s) 430, 720, 808, 823, 1341
MaeIII GTNAC 6 cut(s) 445, 716, 824, 1420, 2549, 2993
MfeI CAATTG 5 cut(s) 186, 207, 914, 1466, 1479
MflI RGATCY 3 cut(s) 1412, 2126, 3049
MhlI GDGCHC 2 cut(s) 436, 1582
MlyI GAGTC 3 cut(s) 2698, 3209, 3336
MmeI TCCRAC 3 cut(s) 1075, 1411, 3183
Mph1103I ATGCAT 3 cut(s) 1036, 1452, 3022
MroXI GAANNNNTTC 4 cut(s) 516, 531, 1104, 2884
MslI CAYNNNNRTG 2 cut(s) 2505, 3305
MspA1I CMGCKG 2 cut(s) 81, 1060
MspCI CTTAAG 1 cut(s) 1262
MspI CCGG 4 cut(s) 133, 407, 2579, 3070
MunI CAATTG 5 cut(s) 186, 207, 914, 1466, 1479
Mva1269I GAATGC 2 cut(s) 278, 1640
MwoI GCNNNNNNNGC 6 cut(s) 1456, 1518, 1586, 1815, 2671, 3026
NcoI CCATGG 1 cut(s) 239
NdeI CATATG 1 cut(s) 1689
NlaIV GGNNCC 5 cut(s) 1533, 1702, 1901, 2961, 3094
NmuCI GTSAC 4 cut(s) 716, 824, 2549, 2993
NsbI TGCGCA 1 cut(s) 419
NsiI ATGCAT 3 cut(s) 1036, 1452, 3022
NspI RCATGY 2 cut(s) 1323, 1919
NspV TTCGAA 2 cut(s) 2071, 2677
OliI CACNNNNGTG 1 cut(s) 2505
PaeI GCATGC 1 cut(s) 1323
PaeR7I CTCGAG 2 cut(s) 170, 1556
PagI TCATGA 1 cut(s) 481
PaqCI CACCTGC 1 cut(s) 411
PceI AGGCCT 1 cut(s) 392
PciSI GCTCTTC 4 cut(s) 1059, 1164, 1497, 2352
PctI GAATGC 2 cut(s) 278, 1640
PdmI GAANNNNTTC 4 cut(s) 516, 531, 1104, 2884
PfeI GAWTC 9 cut(s) 194, 325, 540, 575, 743, 963, 2011, 2510, 3139
PflMI CCANNNNNTGG 2 cut(s) 240, 1388
PfoI TCCNGGA 3 cut(s) 619, 1883, 2254
PkrI GCNGC 8 cut(s) 937, 1141, 1174, 1461, 1817, 2371, 2594, 3179
PleI GAGTC 3 cut(s) 2698, 3208, 3335
PpsI GAGTC 3 cut(s) 2698, 3208, 3335
Ppu21I YACGTR 1 cut(s) 824
PpuMI RGGWCCY 1 cut(s) 1899
PshBI ATTAAT 2 cut(s) 1937, 2805
Psp5II RGGWCCY 1 cut(s) 1899
PspFI CCCAGC 1 cut(s) 2372
PspN4I GGNNCC 5 cut(s) 1533, 1702, 1901, 2961, 3094
PspPI GGNCC 3 cut(s) 1087, 1700, 1899
PspPPI RGGWCCY 1 cut(s) 1899
PstI CTGCAG 2 cut(s) 2893, 3179
PstNI CAGNNNCTG 1 cut(s) 2553
PsuI RGATCY 3 cut(s) 1412, 2126, 3049
PvuII CAGCTG 1 cut(s) 1060
RsaI GTAC 8 cut(s) 303, 339, 552, 602, 631, 822, 1554, 1954
RsaNI GTAC 8 cut(s) 302, 338, 551, 601, 630, 821, 1553, 1953
RseI CAYNNNNRTG 2 cut(s) 2505, 3305
SapI GCTCTTC 4 cut(s) 1059, 1164, 1497, 2352
SatI GCNGC 8 cut(s) 936, 1140, 1173, 1460, 1816, 2370, 2593, 3178
Sau96I GGNCC 3 cut(s) 1087, 1700, 1899
ScaI AGTACT 1 cut(s) 303
SchI GAGTC 3 cut(s) 2698, 3209, 3336
SduI GDGCHC 2 cut(s) 436, 1582
SexAI ACCWGGT 1 cut(s) 1089
SfaNI GCATC 9 cut(s) 241, 1184, 1437, 1641, 2294, 2356, 2664, 2825, 3110
SfcI CTRYAG 5 cut(s) 1752, 1810, 2169, 2889, 3175
Sfr274I CTCGAG 2 cut(s) 170, 1556
SfuI TTCGAA 2 cut(s) 2071, 2677
SinI GGWCC 2 cut(s) 1087, 1899
SlaI CTCGAG 2 cut(s) 170, 1556
SmiMI CAYNNNNRTG 2 cut(s) 2505, 3305
SmlI CTYRAG 8 cut(s) 170, 728, 1109, 1262, 1556, 1661, 2243, 2282
SmoI CTYRAG 8 cut(s) 170, 728, 1109, 1262, 1556, 1661, 2243, 2282
SphI GCATGC 1 cut(s) 1323
SseBI AGGCCT 1 cut(s) 392
SsiI CCGC 4 cut(s) 81, 1535, 2369, 2763
SspI AATATT 1 cut(s) 2296
SspMI CTAG 8 cut(s) 537, 834, 870, 908, 939, 1283, 1364, 1418
StuI AGGCCT 1 cut(s) 392
StyI CCWWGG 1 cut(s) 239
TaaI ACNGT 6 cut(s) 62, 634, 847, 1426, 1864, 2555
TaiI ACGT 5 cut(s) 433, 723, 811, 826, 1344
TatI WGTACW 3 cut(s) 301, 1552, 1952
TauI GCSGC 1 cut(s) 2372
TfiI GAWTC 9 cut(s) 194, 325, 540, 575, 743, 963, 2011, 2510, 3139
TscAI CASTG 7 cut(s) 1587, 1631, 2554, 2929, 2950, 3109, 3307
TseFI GTSAC 4 cut(s) 716, 824, 2549, 2993
TseI GCWGC 7 cut(s) 935, 1139, 1172, 1459, 1815, 2592, 3177
Tsp45I GTSAC 4 cut(s) 716, 824, 2549, 2993
TspDTI ATGAA 8 cut(s) 305, 399, 498, 705, 918, 1268, 1706, 2718
TspGWI ACGGA 4 cut(s) 1172, 1667, 3032, 3320
TspRI CASTG 7 cut(s) 1587, 1631, 2554, 2929, 2950, 3109, 3307
Van91I CCANNNNNTGG 2 cut(s) 240, 1388
Vha464I CTTAAG 1 cut(s) 1262
VneI GTGCAC 1 cut(s) 1578
VpaK11BI GGWCC 2 cut(s) 1087, 1899
VspI ATTAAT 2 cut(s) 1937, 2805
XagI CCTNNNNNAGG 1 cut(s) 2889
XapI RAATTY 7 cut(s) 100, 527, 1771, 2073, 2221, 2686, 2880
XceI RCATGY 2 cut(s) 1323, 1919
XcmI CCANNNNNNNNNTGG 1 cut(s) 1987
XhoI CTCGAG 2 cut(s) 170, 1556
XmnI GAANNNNTTC 4 cut(s) 516, 531, 1104, 2884
XspI CTAG 8 cut(s) 537, 834, 870, 908, 939, 1283, 1364, 1418
ZrmI AGTACT 1 cut(s) 303
Zsp2I ATGCAT 3 cut(s) 1036, 1452, 3022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.