Rorug02G0096600

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000105
Physical Location & Seq
Reverse (-)
7940608 .. 7948398
7791 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0096600.1

Sequence Viewer

Length: 3840 bp
ATGGAAGCCATTGAAATGGAAGAGCTGCCGCTAACCCCCACCAGCACCACCGCCTTCGACGACGACGCCGACGCCGACGCCGACATCTGCACCTCAATCCTCACGCGCTTCGGCAACTCCACGCGCGAGGACCACCAGCACCTCTGCGCCGTCATCGGCGGCATGGCCCAGGGTTTCAAAGACCAGAACCTCCCTCCGTCTCCGGTCGCCTACTTCGGCGCCGCGTGCTCGTCTCTCGATCGAATCTTATCGGAGCCGGAGCCCTCCGGTCACATGATCGACGCGCTCCTCACAATTCTCTCCATGGCCGTCCGGAAAGTCTCGCCGGCGATTCTCGTGAAGAAGAGCGACCTCGTTAACGGACTCCTGGTTCGTGCTCTCCTGTCCAGCTCTTTGACCGTCGCCGGAGCCGTCTCCGGGTTGAAATGTATAGCGCATTTGCTCATCGTCGGCAGCCGGGTCAACCACAACAACTGGTCAGACATTTCTCAGCTCTATGGCTTCTTACTGAGCTTCGCTACCGATTCCCGCCCTAAGGTGAGAAGGCAATCACATTTGCGGTTACGTGACGTACTGCAAAGTTTCCAAGGGACTTCGCTGCACTCACCGGCAAGTCAAGGCATTACTGATTTGTTCAAAAGGTTTATTCTGCTTGCTGGTGGAACAAAACCAGCTGCTAGTGAAGGGCCAAAAGGGAGTCTCGAGGTTTTATATCTTTTGGATGCTTTTAAAGAATGTCTTGCTCTTATGTCGACCAAGAATAAAAACGAGATCCTCGAGCTCTTTAAACCTCTCTTGGGACTGCAAAACCCTGTTGTTACAAGGCGCATAACGGATGGTTTGTATAGGCTTTGTCTCGAACCATGCCCAGATGTTTCTCCTCAGATATTGCTTGATCAACTATGCTTATTATCTCTTTCTGTCTCCAACAATAAGACATCTGTGGATGACATGACATTTACGGCTCGTTTGCTCAATATTGGAATGGCAAAGGTTTATTCACTCAACAGGCAATTGTGCGTGATTAAACTCCCTACTGTATTCAGTGCACTACGAGATATATTGGGTTCTGAGCATGAAGAGGCCATTCATGCAGCAGTGAACGCATTTAAGAGTCTTATTCATGCTTGCATTGATGAAAGCTTGATCAAACAGGGGGTTGACCAGATTGTGAAGAATGGAAATATGGATGAATGCAGGTCTGGGCCAACTGTAATTGAAAAAGTGTGTGCTATTATTGAAAGCTTGCTTGGCTACCACTACACTCCTGTCTTGGACCTTGCATTTCAAGTTGTTTCAGCGATGTTTGATAAATTAGGGGTATACTCTTCTTATTTCATGAGGGGGACACTGAAGAGCTTGGCAGACATGGAAAAGCTGCCTGATGAAGATTTCCCTTTCAGGAAAGAGCTGCATGAATGCCTCGGAACAGCTCTTGTTGCGATGGGACCTGAAACTTTTATAGGCTTTCTGCCTCTTAATTTGGAGGCTGAAGACCTTAATGAGGTGAATGTTTGGCTTTTTCCAATATTGAAGCAGTATACAATTGGTGCACGATTGAGCTTTTTTACGGAGTCAATTTTGGGTATGGTCGAGGTGATACGGAACAGATCTCGACAGCTTGAGTCACAAGGACGTATTTTTTCATCAAGGAGTACAGACGCACTTATATACTCCTTGTGGTCACTGTTACCTTCCTTTTGCAACTTCCCTTCTGATACTGCTGAGAGTTTCAACGATCTGAAGCAAGCTTTGTGCAACGCCCTTCGTGATGAACCTGAGATTCGTGGACTAATATGCTTGAGCTTGCAAATTCTTGTTCAACAAAATAAGAAAATTGTGGAAGAAGTGAATGATCTGTCTGATAGCGAAGTAGGTACTGCCAAACAGAGAGCGATGGCTAATTATACCCCTCAGGTTATGACAGATAACTTGAGCGTGCTCAAATCAAGCGCGCGCGAGATCTTGACTGTTCTATCAGGTGTCTTCTTGAATTCTACAAAAGATGATGGAGGTTGTTTACAGTCCACAATTGGTGAGTTTGCTTCAATATCAGATAAAGCAGTTGTGTCAAGGTTGTTTCTAAGTAATATGCACAAGCTTTTGAGGGTCACTAAAGAGGCCAGGGCAGCTGCAAGTTCTAGTGATTCTAATTCCATGCAAAGGGCACTACTATTTGACTTAGCAGTTTCATTTTTACCTGGTTTAAATGCTGATGAGGTTGACGTGTTGTTTAACGCAATAAAGCCTGCATTACAGGATGACGAAGGTTTGATACAGAAGAAGGCGTACAAAGTTCTTTCAATCATTCTTCGGGACTTTGATGGGTTTATCTCTTCGAAGCTCGAGGATTTGCTTAGACTGATGGTGGAATTGTTGCCTTCATGCCATTTTTCAGCTAGACGGCATAGACTTGACTGCTTGTACCTCCTAATAGTCCATGTTTCGAAGAGCGAGACAGAGCAGAGGTGGCATGACGTCATAAGTTCTTTCCTGACAGAGATAATTCTTGGGCTTAAAGAGGCTAACAAGAAAACAAGAAACAAAGCATATGATATTCTAGTCCAGATCGGCCATGCTTGTGGGGATGAGGAGAAAGGCGGGAAGAAAGAGAACCTGTATCAATTCTTTAACATGGTAGCTGGAGGCCTGGCTGGCGAAACCCCTGTTATAATCAGTGCTGCAATGAGAGGCTTAGCTAGGTTGGCTTATGAGTTTTCTGACCTTGTGTCTTCTGCTACCAATTTGCTTCCATCAACGTTTCTGCTCCTCCAGAGAAAGAACAGAGAAATAATTAAAGCTAATTTAGGCTTGCTGAAGGTATTGGTTGCCAAATCACAAGCTGAGGGGTTGCAATTGCACTTGAAAAGCATGGTTGAAGCCTTGTTGAAGTGGCAAGATGATACTAAAACTCATTTCAAAGCCAAGATTAAGCTTCTACTAGAAATGCTCGTCAAGAAATGTGGGCTGGATGCTGTTAAGGCTGTCATGCCTCAAGAGCACATGAAACTACTTACCAACATTCGGAAGATTAAGGAACGAAAAGATAAGAAACAACAGGCTTCTAGATCGGAGGAAGCTAAATCTCATGCATCCAAAGCAACTACATCCAGGCTAAGCAGATGGAACCATTCAAAAGTATTTTCTGATTTTGGTGATGAAGAAACTGATGATAGTAATACGGATTATATGGATTCCCAAACAGTCACTGGCCGGCGTGGCAAGGCTTCACACCTCAAATCTAAAGCATCTTCATCATGGGCCAAGAGTAGAACAAATAAGAACTTGCCTGACCACTTATTTAATCAACTAGAAGACGAGCCTCTTGACTTGCTTGATCGGCAAAGAACAAGGTCCGCACTTCGATCATCAGAGAATTTAAAACGTAAAATGGAGTCTGATGAAGGGCCAGAGATAGACCCTGATGGGCGCCTCATAATTCATGAAGAATCAAACTCGTACAACAAAAAGCCATCTCATCCTGATTCAGATGCAAGGAGTGAAGCAGGAAGTCACTTGTCTGTGAATACAAAGAAAATACAGAAGCGAAGGAAGACATCAGAATCTGGATGGGCTTCAACTGGCAATGAGTATGCCAGCAAGAAGGCTGGTGGGGATTTGAAAAAGAAGGACAAGCTTGAACCATACGCGTATTGGCCACTTGATCGCAAGATGATGAGCCGCAGACCAGAGCATAGGGCTGCTGCCAGGAAAGGGATATCAAGTGTGGTTAGGATGACAAAGAAGCTTGAAGGGAAGAGTGCCTCAAGTATACTCACCAGTAAAGGCTTGAAGTTTAAAAGGGTCCAGAAGAAGGGCAGCAAGAGAAAAAGTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1279

Amino Acids

142.1

Weight (kDa)

8.86

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEAT_RRP12_N PF25772 21 - 291 1.9e-102 RRP12-like, N-terminal HEAT repeats
RRP12_HEAT PF08161 357 - 664 7.5e-76 Ribosomal RNA-processing protein 12, HEAT repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2672
AatII GACGTC 1 cut(s) 2481
Acc36I ACCTGC 1 cut(s) 1188
AccB1I GGYRCC 2 cut(s) 218, 3429
AccI GTMKAC 4 cut(s) 752, 1323, 1541, 3772
AccII CGCG 9 cut(s) 106, 124, 126, 224, 284, 1955, 1957, 1959, 3650
AccIII TCCGGA 1 cut(s) 312
AciI CCGC 9 cut(s) 29, 51, 159, 222, 529, 559, 2601, 3357, 3682
AclI AACGTT 1 cut(s) 2759
AclWI GGATC 1 cut(s) 766
AcoI YGGCCR 4 cut(s) 306, 2572, 3211, 3656
AcsI RAATTY 3 cut(s) 1812, 1993, 3376
AcuI CTGAAG 4 cut(s) 1373, 1512, 1763, 2837
AcyI GRCGYC 6 cut(s) 66, 72, 78, 219, 2478, 3430
AfaI GTAC 6 cut(s) 573, 1657, 1879, 2291, 2426, 3461
AfiI CCNNNNNNNGG 8 cut(s) 417, 535, 797, 1504, 2163, 3024, 3714, 3814
AflIII ACRYGT 2 cut(s) 2226, 3648
AhdI GACNNNNNGTC 1 cut(s) 2728
AjiI CACGTC 1 cut(s) 2227
AjnI CCWGG 7 cut(s) 168, 366, 2123, 2200, 2649, 3110, 3707
AjuI GAANNNNNNNTTGG 2 cut(s) 1233, 1265
AloI GAACNNNNNNTCC 2 cut(s) 354, 386
Alw21I GWGCWC 7 cut(s) 230, 379, 783, 1051, 1555, 1944, 3003
Alw26I GTCTC 8 cut(s) 204, 237, 325, 418, 704, 860, 928, 2450
Alw44I GTGCAC 2 cut(s) 1047, 1551
AlwI GGATC 1 cut(s) 766
AlwNI CAGNNNCTG 2 cut(s) 3209, 3566
Ama87I CYCGRG 3 cut(s) 701, 776, 2345
Aor13HI TCCGGA 1 cut(s) 312
ApaLI GTGCAC 2 cut(s) 1047, 1551
ApoI RAATTY 3 cut(s) 1812, 1993, 3376
ArsI GACNNNNNNTTYG 2 cut(s) 354, 386
AsuC2I CCSGG 2 cut(s) 418, 458
AsuHPI GGTGA 7 cut(s) 550, 597, 1519, 1609, 2048, 3167, 3769
AsuII TTCGAA 2 cut(s) 2339, 2447
AvaI CYCGRG 3 cut(s) 701, 776, 2345
AvaII GGWCC 5 cut(s) 130, 1276, 1448, 3354, 3805
AxyI CCTNAGG 2 cut(s) 534, 1914
BaeGI GKGCMC 3 cut(s) 1051, 1555, 2170
BalI TGGCCA 1 cut(s) 3658
BanI GGYRCC 2 cut(s) 218, 3429
BanII GRGCYC 2 cut(s) 264, 783
BarI GAAGNNNNNNTAC 4 cut(s) 2259, 2273, 2291, 2305
BauI CACGAG 1 cut(s) 335
BbsI GAAGAC 5 cut(s) 1500, 1978, 2724, 3321, 3560
Bbv12I GWGCWC 7 cut(s) 230, 379, 783, 1051, 1555, 1944, 3003
BbvCI CCTCAGC 1 cut(s) 2844
BceAI ACGGC 5 cut(s) 134, 293, 395, 978, 2420
BciT130I CCWGG 7 cut(s) 170, 368, 2125, 2202, 2651, 3112, 3709
BclI TGATCA 2 cut(s) 895, 1146
BcnI CCSGG 2 cut(s) 418, 458
BcoDI GTCTC 8 cut(s) 204, 237, 325, 418, 704, 860, 928, 2450
BfaI CTAG 8 cut(s) 678, 2142, 2400, 2561, 2700, 2942, 3066, 3311
BfoI RGCGCY 2 cut(s) 222, 3433
BfuAI ACCTGC 1 cut(s) 1188
BglI GCCNNNNNGGC 2 cut(s) 2655, 3219
BglII AGATCT 2 cut(s) 1610, 1962
BlpI GCTNAGC 2 cut(s) 2695, 3116
Bme1390I CCNGG 9 cut(s) 170, 368, 418, 458, 2125, 2202, 2651, 3112, 3709
Bme18I GGWCC 5 cut(s) 130, 1276, 1448, 3354, 3805
BmeRI GACNNNNNGTC 1 cut(s) 2728
BmeT110I CYCGRG 3 cut(s) 701, 776, 2345
BmgBI CACGTC 1 cut(s) 2227
BmiI GGNNCC 8 cut(s) 220, 255, 261, 409, 1449, 3128, 3431, 3806
BmrFI CCNGG 9 cut(s) 170, 368, 418, 458, 2125, 2202, 2651, 3112, 3709
BmsI GCATC 5 cut(s) 712, 2962, 3101, 3257, 3481
BpiI GAAGAC 5 cut(s) 1500, 1978, 2724, 3321, 3560
BpmI CTGGAG 2 cut(s) 2664, 2756
Bpu10I CCTNAGC 1 cut(s) 2844
Bpu1102I GCTNAGC 2 cut(s) 2695, 3116
Bpu14I TTCGAA 2 cut(s) 2339, 2447
BpuEI CTTGAG 5 cut(s) 1643, 1822, 1954, 2979, 3751
BpuMI CCSGG 2 cut(s) 418, 458
BsaAI YACGTR 1 cut(s) 566
BsaHI GRCGYC 6 cut(s) 66, 72, 78, 219, 2478, 3430
BsaJI CCNNGG 6 cut(s) 168, 169, 303, 586, 1423, 2124
BsaWI WCCGGW 3 cut(s) 202, 266, 312
Bsc4I CCNNNNNNNGG 8 cut(s) 417, 535, 797, 1504, 2163, 3024, 3714, 3814
Bse118I RCCGGY 3 cut(s) 325, 607, 3213
Bse1I ACTGG 4 cut(s) 479, 3214, 3586, 3780
Bse21I CCTNAGG 2 cut(s) 534, 1914
Bse3DI GCAATG 2 cut(s) 2691, 3592
BseAI TCCGGA 1 cut(s) 312
BseBI CCWGG 7 cut(s) 170, 368, 2125, 2202, 2651, 3112, 3709
BseDI CCNNGG 6 cut(s) 168, 169, 303, 586, 1423, 2124
BseLI CCNNNNNNNGG 8 cut(s) 417, 535, 797, 1504, 2163, 3024, 3714, 3814
BseMI GCAATG 2 cut(s) 2691, 3592
BseMII CTCAG 8 cut(s) 500, 503, 896, 1062, 1716, 1770, 1928, 2835
BseNI ACTGG 4 cut(s) 479, 3214, 3586, 3780
BsePI GCGCGC 2 cut(s) 1953, 1955
BseRI GAGGAG 4 cut(s) 278, 870, 2606, 2759
BseSI GKGCMC 3 cut(s) 1051, 1555, 2170
BsgI GTGCAG 2 cut(s) 73, 584
Bsh1236I CGCG 9 cut(s) 106, 124, 126, 224, 284, 1955, 1957, 1959, 3650
Bsh1285I CGRYCG 2 cut(s) 207, 241
BshNI GGYRCC 2 cut(s) 218, 3429
BsiEI CGRYCG 2 cut(s) 207, 241
BsiHKAI GWGCWC 7 cut(s) 230, 379, 783, 1051, 1555, 1944, 3003
BsiHKCI CYCGRG 3 cut(s) 701, 776, 2345
BslFI GGGAC 5 cut(s) 604, 813, 1360, 1461, 2330
BslI CCNNNNNNNGG 8 cut(s) 417, 535, 797, 1504, 2163, 3024, 3714, 3814
BsmAI GTCTC 8 cut(s) 204, 237, 325, 418, 704, 860, 928, 2450
BsmBI CGTCTC 3 cut(s) 204, 237, 418
BsmFI GGGAC 5 cut(s) 604, 813, 1360, 1461, 2330
BsmI GAATGC 2 cut(s) 1199, 1424
BsoBI CYCGRG 3 cut(s) 701, 776, 2345
Bsp119I TTCGAA 2 cut(s) 2339, 2447
Bsp1286I GDGCHC 9 cut(s) 230, 264, 379, 783, 1051, 1555, 1944, 2170, 3003
Bsp13I TCCGGA 1 cut(s) 312
Bsp1720I GCTNAGC 2 cut(s) 2695, 3116
Bsp19I CCATGG 1 cut(s) 303
BspACI CCGC 9 cut(s) 29, 51, 159, 222, 529, 559, 2601, 3357, 3682
BspCNI CTCAG 8 cut(s) 501, 502, 895, 1063, 1717, 1771, 1927, 2836
BspEI TCCGGA 1 cut(s) 312
BspFNI CGCG 9 cut(s) 106, 124, 126, 224, 284, 1955, 1957, 1959, 3650
BspHI TCATGA 2 cut(s) 1338, 3442
BspLI GGNNCC 8 cut(s) 220, 255, 261, 409, 1449, 3128, 3431, 3806
BspMI ACCTGC 1 cut(s) 1188
BspPI GGATC 1 cut(s) 766
BspQI GCTCTTC 4 cut(s) 15, 338, 1349, 2444
BspT104I TTCGAA 2 cut(s) 2339, 2447
BspT107I GGYRCC 2 cut(s) 218, 3429
BsrDI GCAATG 2 cut(s) 2691, 3592
BsrFI RCCGGY 3 cut(s) 325, 607, 3213
BsrI ACTGG 4 cut(s) 479, 3214, 3586, 3780
BssAI RCCGGY 3 cut(s) 325, 607, 3213
BssECI CCNNGG 6 cut(s) 168, 169, 303, 586, 1423, 2124
BssHII GCGCGC 2 cut(s) 1953, 1955
BssNAI GTATAC 3 cut(s) 1324, 1542, 3773
BssNI GRCGYC 6 cut(s) 66, 72, 78, 219, 2478, 3430
BssSI CACGAG 1 cut(s) 335
BssT1I CCWWGG 2 cut(s) 303, 586
Bst1107I GTATAC 3 cut(s) 1324, 1542, 3773
Bst2BI CACGAG 1 cut(s) 335
Bst2UI CCWGG 7 cut(s) 170, 368, 2125, 2202, 2651, 3112, 3709
Bst4CI ACNGT 7 cut(s) 400, 1039, 1213, 1689, 1972, 2025, 3205
Bst6I CTCTTC 8 cut(s) 15, 338, 1074, 1333, 1349, 2341, 2444, 3752
BstACI GRCGYC 6 cut(s) 66, 72, 78, 219, 2478, 3430
BstBAI YACGTR 1 cut(s) 566
BstBI TTCGAA 2 cut(s) 2339, 2447
BstDSI CCRYGG 1 cut(s) 303
BstENI CCTNNNNNAGG 1 cut(s) 1502
BstFNI CGCG 9 cut(s) 106, 124, 126, 224, 284, 1955, 1957, 1959, 3650
BstH2I RGCGCY 2 cut(s) 222, 3433
BstMAI GTCTC 8 cut(s) 204, 237, 325, 418, 704, 860, 928, 2450
BstMCI CGRYCG 2 cut(s) 207, 241
BstNI CCWGG 7 cut(s) 170, 368, 2125, 2202, 2651, 3112, 3709
BstSCI CCNGG 9 cut(s) 168, 366, 416, 456, 2123, 2200, 2649, 3110, 3707
BstSLI GKGCMC 3 cut(s) 1051, 1555, 2170
BstUI CGCG 9 cut(s) 106, 124, 126, 224, 284, 1955, 1957, 1959, 3650
BstV2I GAAGAC 5 cut(s) 1500, 1978, 2724, 3321, 3560
BstX2I RGATCY 3 cut(s) 771, 1610, 1962
BstXI CCANNNNNNTGG 2 cut(s) 16, 2582
BstYI RGATCY 3 cut(s) 771, 1610, 1962
BstZ17I GTATAC 3 cut(s) 1324, 1542, 3773
Bsu36I CCTNAGG 2 cut(s) 534, 1914
BtgI CCRYGG 1 cut(s) 303
BtgZI GCGATG 3 cut(s) 1316, 1457, 1910
BtrI CACGTC 1 cut(s) 2227
BtsI GCAGTG 1 cut(s) 1104
BtsIMutI CAGTG 6 cut(s) 1051, 1104, 1349, 1685, 2683, 3207
BveI ACCTGC 1 cut(s) 1188
CaiI CAGNNNCTG 2 cut(s) 3209, 3566
CciI TCATGA 2 cut(s) 1338, 3442
Cfr10I RCCGGY 3 cut(s) 325, 607, 3213
CseI GACGC 5 cut(s) 74, 80, 86, 290, 1670
CsiI ACCWGGT 1 cut(s) 2200
Csp6I GTAC 6 cut(s) 572, 1656, 1878, 2290, 2425, 3460
CspCI CAANNNNNGTGG 2 cut(s) 2944, 2979
CviQI GTAC 6 cut(s) 572, 1656, 1878, 2290, 2425, 3460
DinI GGCGCC 2 cut(s) 220, 3431
DraI TTTAAA 5 cut(s) 730, 787, 2208, 3381, 3799
DriI GACNNNNNGTC 1 cut(s) 2728
EaeI YGGCCR 4 cut(s) 306, 2572, 3211, 3656
Eam1104I CTCTTC 8 cut(s) 15, 338, 1074, 1333, 1349, 2341, 2444, 3752
Eam1105I GACNNNNNGTC 1 cut(s) 2728
EarI CTCTTC 8 cut(s) 15, 338, 1074, 1333, 1349, 2341, 2444, 3752
Ecl136II GAGCTC 1 cut(s) 781
Eco130I CCWWGG 2 cut(s) 303, 586
Eco147I AGGCCT 1 cut(s) 2649
Eco24I GRGCYC 2 cut(s) 264, 783
Eco32I GATATC 1 cut(s) 3720
Eco47I GGWCC 5 cut(s) 130, 1276, 1448, 3354, 3805
Eco53kI GAGCTC 1 cut(s) 781
Eco57I CTGAAG 4 cut(s) 1373, 1512, 1763, 2837
Eco81I CCTNAGG 2 cut(s) 534, 1914
Eco88I CYCGRG 3 cut(s) 701, 776, 2345
EcoICRI GAGCTC 1 cut(s) 781
EcoNI CCTNNNNNAGG 1 cut(s) 1502
EcoO109I RGGNCCY 1 cut(s) 1448
EcoRI GAATTC 1 cut(s) 1993
EcoRII CCWGG 7 cut(s) 168, 366, 2123, 2200, 2649, 3110, 3707
EcoRV GATATC 1 cut(s) 3720
EcoT14I CCWWGG 2 cut(s) 303, 586
EcoT22I ATGCAT 1 cut(s) 3094
EcoT38I GRGCYC 2 cut(s) 264, 783
EgeI GGCGCC 2 cut(s) 220, 3431
EheI GGCGCC 2 cut(s) 220, 3431
ErhI CCWWGG 2 cut(s) 303, 586
Esp3I CGTCTC 3 cut(s) 204, 237, 418
FalI AAGNNNNNCTT 2 cut(s) 723, 755
FaqI GGGAC 5 cut(s) 604, 813, 1360, 1461, 2330
FauI CCCGC 2 cut(s) 536, 2594
FauNDI CATATG 1 cut(s) 2551
FbaI TGATCA 2 cut(s) 895, 1146
FblI GTMKAC 4 cut(s) 752, 1323, 1541, 3772
FriOI GRGCYC 2 cut(s) 264, 783
FspBI CTAG 8 cut(s) 678, 2142, 2400, 2561, 2700, 2942, 3066, 3311
GsuI CTGGAG 2 cut(s) 2664, 2756
HaeII RGCGCY 2 cut(s) 222, 3433
HgaI GACGC 5 cut(s) 74, 80, 86, 290, 1670
Hin1I GRCGYC 6 cut(s) 66, 72, 78, 219, 2478, 3430
HincII GTYRAC 5 cut(s) 358, 463, 753, 1162, 2224
HindII GTYRAC 5 cut(s) 358, 463, 753, 1162, 2224
HindIII AAGCTT 7 cut(s) 1141, 1243, 1749, 2099, 2933, 3635, 3746
HpaI GTTAAC 1 cut(s) 358
HphI GGTGA 7 cut(s) 550, 597, 1519, 1609, 2048, 3167, 3769
Hpy99I CGWCG 8 cut(s) 62, 65, 68, 74, 80, 284, 404, 452
HpyCH4III ACNGT 7 cut(s) 400, 1039, 1213, 1689, 1972, 2025, 3205
HpyCH4IV ACGT 7 cut(s) 565, 570, 1636, 2226, 2478, 2759, 3385
HpySE526I ACGT 7 cut(s) 565, 570, 1636, 2226, 2478, 2759, 3385
Hsp92I GRCGYC 6 cut(s) 66, 72, 78, 219, 2478, 3430
KasI GGCGCC 2 cut(s) 218, 3429
Kpn2I TCCGGA 1 cut(s) 312
KroI GCCGGC 2 cut(s) 325, 3213
KroNI GCCGGC 2 cut(s) 327, 3215
Ksp22I TGATCA 2 cut(s) 895, 1146
KspAI GTTAAC 1 cut(s) 358
LguI GCTCTTC 4 cut(s) 15, 338, 1349, 2444
LmnI GCTCC 5 cut(s) 253, 259, 291, 407, 2772
LweI GCATC 5 cut(s) 712, 2962, 3101, 3257, 3481
MabI ACCWGGT 1 cut(s) 2200
MaeI CTAG 8 cut(s) 678, 2142, 2400, 2561, 2700, 2942, 3066, 3311
MaeII ACGT 7 cut(s) 565, 570, 1636, 2226, 2478, 2759, 3385
MauBI CGCGCGCG 1 cut(s) 1955
MfeI CAATTG 4 cut(s) 1013, 1545, 2031, 2855
MflI RGATCY 3 cut(s) 771, 1610, 1962
MhlI GDGCHC 9 cut(s) 230, 264, 379, 783, 1051, 1555, 1944, 2170, 3003
MlsI TGGCCA 1 cut(s) 3658
MluI ACGCGT 1 cut(s) 3648
MluNI TGGCCA 1 cut(s) 3658
Mly113I GGCGCC 2 cut(s) 219, 3430
MlyI GAGTC 6 cut(s) 357, 706, 1123, 1583, 1634, 3404
MmeI TCCRAC 1 cut(s) 951
Mox20I TGGCCA 1 cut(s) 3658
Mph1103I ATGCAT 1 cut(s) 3094
MreI CGCCGGCG 1 cut(s) 325
MroI TCCGGA 1 cut(s) 312
MroNI GCCGGC 2 cut(s) 325, 3213
MscI TGGCCA 1 cut(s) 3658
MslI CAYNNNNRTG 2 cut(s) 14, 2580
Msp20I TGGCCA 1 cut(s) 3658
MspA1I CMGCKG 2 cut(s) 674, 2132
MspR9I CCNGG 9 cut(s) 170, 368, 418, 458, 2125, 2202, 2651, 3112, 3709
MunI CAATTG 4 cut(s) 1013, 1545, 2031, 2855
Mva1269I GAATGC 2 cut(s) 1199, 1424
MvaI CCWGG 7 cut(s) 170, 368, 2125, 2202, 2651, 3112, 3709
MvnI CGCG 9 cut(s) 106, 124, 126, 224, 284, 1955, 1957, 1959, 3650
NaeI GCCGGC 2 cut(s) 327, 3215
NarI GGCGCC 2 cut(s) 219, 3430
NciI CCSGG 2 cut(s) 418, 458
NcoI CCATGG 1 cut(s) 303
NdeI CATATG 1 cut(s) 2551
NgoMIV GCCGGC 2 cut(s) 325, 3213
NlaIV GGNNCC 8 cut(s) 220, 255, 261, 409, 1449, 3128, 3431, 3806
NmuCI GTSAC 7 cut(s) 269, 566, 1626, 1683, 2110, 3205, 3512
NsiI ATGCAT 1 cut(s) 3094
NspV TTCGAA 2 cut(s) 2339, 2447
PaeR7I CTCGAG 3 cut(s) 701, 776, 2345
PagI TCATGA 2 cut(s) 1338, 3442
PasI CCCWGGG 1 cut(s) 169
PauI GCGCGC 2 cut(s) 1953, 1955
PceI AGGCCT 1 cut(s) 2649
PciSI GCTCTTC 4 cut(s) 15, 338, 1349, 2444
PcsI WCGNNNNNNNCGW 5 cut(s) 66, 72, 78, 408, 774
PctI GAATGC 2 cut(s) 1199, 1424
PdiI GCCGGC 2 cut(s) 327, 3215
PfeI GAWTC 9 cut(s) 243, 331, 524, 1783, 2147, 3194, 3449, 3485, 3563
Ple19I CGATCG 1 cut(s) 241
PleI GAGTC 6 cut(s) 357, 705, 1122, 1582, 1633, 3403
PluTI GGCGCC 2 cut(s) 222, 3433
PpsI GAGTC 6 cut(s) 357, 705, 1122, 1582, 1633, 3403
Ppu21I YACGTR 1 cut(s) 566
PpuMI RGGWCCY 1 cut(s) 1448
PsiI TTATAA 1 cut(s) 2672
Psp124BI GAGCTC 1 cut(s) 783
Psp1406I AACGTT 1 cut(s) 2759
Psp5II RGGWCCY 1 cut(s) 1448
Psp6I CCWGG 7 cut(s) 168, 366, 2123, 2200, 2649, 3110, 3707
PspGI CCWGG 7 cut(s) 168, 366, 2123, 2200, 2649, 3110, 3707
PspN4I GGNNCC 8 cut(s) 220, 255, 261, 409, 1449, 3128, 3431, 3806
PspPPI RGGWCCY 1 cut(s) 1448
PspXI VCTCGAGB 2 cut(s) 776, 2345
PstNI CAGNNNCTG 2 cut(s) 3209, 3566
PsuI RGATCY 3 cut(s) 771, 1610, 1962
PteI GCGCGC 2 cut(s) 1953, 1955
PvuI CGATCG 1 cut(s) 241
PvuII CAGCTG 2 cut(s) 674, 2132
RsaI GTAC 6 cut(s) 573, 1657, 1879, 2291, 2426, 3461
RsaNI GTAC 6 cut(s) 572, 1656, 1878, 2290, 2425, 3460
RseI CAYNNNNRTG 2 cut(s) 14, 2580
SacI GAGCTC 1 cut(s) 783
SalI GTCGAC 1 cut(s) 751
SapI GCTCTTC 4 cut(s) 15, 338, 1349, 2444
SchI GAGTC 6 cut(s) 357, 706, 1123, 1583, 1634, 3404
ScrFI CCNGG 9 cut(s) 170, 368, 418, 458, 2125, 2202, 2651, 3112, 3709
SduI GDGCHC 9 cut(s) 230, 264, 379, 783, 1051, 1555, 1944, 2170, 3003
SexAI ACCWGGT 1 cut(s) 2200
SfaNI GCATC 5 cut(s) 712, 2962, 3101, 3257, 3481
SfoI GGCGCC 2 cut(s) 220, 3431
Sfr274I CTCGAG 3 cut(s) 701, 776, 2345
SfuI TTCGAA 2 cut(s) 2339, 2447
SgrAI CRCCGGYG 1 cut(s) 325
SinI GGWCC 5 cut(s) 130, 1276, 1448, 3354, 3805
SlaI CTCGAG 3 cut(s) 701, 776, 2345
SmiMI CAYNNNNRTG 2 cut(s) 14, 2580
SmlI CTYRAG 8 cut(s) 701, 776, 1622, 1801, 1933, 2345, 2994, 3766
SmoI CTYRAG 8 cut(s) 701, 776, 1622, 1801, 1933, 2345, 2994, 3766
SseBI AGGCCT 1 cut(s) 2649
SsiI CCGC 9 cut(s) 29, 51, 159, 222, 529, 559, 2601, 3357, 3682
SspDI GGCGCC 2 cut(s) 218, 3429
SspI AATATT 2 cut(s) 979, 1530
SspMI CTAG 8 cut(s) 678, 2142, 2400, 2561, 2700, 2942, 3066, 3311
SstI GAGCTC 1 cut(s) 783
StuI AGGCCT 1 cut(s) 2649
StyD4I CCNGG 9 cut(s) 168, 366, 416, 456, 2123, 2200, 2649, 3110, 3707
StyI CCWWGG 2 cut(s) 303, 586
TaaI ACNGT 7 cut(s) 400, 1039, 1213, 1689, 1972, 2025, 3205
TaiI ACGT 7 cut(s) 568, 573, 1639, 2229, 2481, 2762, 3388
TatI WGTACW 1 cut(s) 1655
TauI GCSGC 4 cut(s) 31, 162, 224, 3684
TfiI GAWTC 9 cut(s) 243, 331, 524, 1783, 2147, 3194, 3449, 3485, 3563
TscAI CASTG 6 cut(s) 1051, 1104, 1356, 1692, 2683, 3214
TseFI GTSAC 7 cut(s) 269, 566, 1626, 1683, 2110, 3205, 3512
Tsp45I GTSAC 7 cut(s) 269, 566, 1626, 1683, 2110, 3205, 3512
TspGWI ACGGA 6 cut(s) 186, 375, 848, 1586, 1618, 3197
TspRI CASTG 6 cut(s) 1051, 1104, 1356, 1692, 2683, 3214
VneI GTGCAC 2 cut(s) 1047, 1551
VpaK11BI GGWCC 5 cut(s) 130, 1276, 1448, 3354, 3805
XagI CCTNNNNNAGG 1 cut(s) 1502
XapI RAATTY 3 cut(s) 1812, 1993, 3376
XbaI TCTAGA 1 cut(s) 3065
XcmI CCANNNNNNNNNTGG 2 cut(s) 3206, 3651
XhoI CTCGAG 3 cut(s) 701, 776, 2345
XmiI GTMKAC 4 cut(s) 752, 1323, 1541, 3772
XspI CTAG 8 cut(s) 678, 2142, 2400, 2561, 2700, 2942, 3066, 3311
ZraI GACGTC 1 cut(s) 2479
Zsp2I ATGCAT 1 cut(s) 3094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.