RLG00000015528

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000105
Physical Location & Seq
Reverse (-)
67567973 .. 67571568
3596 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015528

Sequence Viewer

Length: 2706 bp
ATGACGTCAACTTGGTATCCTCGTCGACGATGGAAATACGATGTTTTTCTAAGCTTCAGAGGGGAAGATACCCGCGAGACTTTCACCTGCCATCTGTATCGTGCGCTAACTGCGAAAGGAGTTATAACATTTCTAGATGACGAAAGCCTGGAGAGAGGATCAACCATTGGCCCATCACTTTTTACAGCAATTCAGGACTCAAGATCAGCCCTTGTCGTTCTTTCAGAGAATTTCGCTTCTTCATCCTGGTGCTTGGATGAACTCTCGAAGATTGTTCAATGCTTGGTAGAGAGGGGACGATTAGTTATCCCGATCTTCTACCGAGTGGCTCCCTCTCATGTGCGCCACCAAAGGGGAAGTTTTCAGCTCCGTACACTAGAGGTTAGGGAACATGAAGAAGTTTATGGGAATAAGGACAAACCAAAGGAGTGGAGACATGCTTTGACAAAGATGGGCAATTTTGTTGGGTTTGATTCGAAAAACTATGAGGATGACGGAAAATTGGTTGAGGCCATTGTTGAGTATACACAAAAATTTCTACATGATACTTATACCTCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGTATTGATGATTTATTAAGCAATTACATATGTCCGCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATGAAATTTGTCAGGATTTTGATAGAAGCTGCATTCTTTACAATGTTAGAGAAAAATCTGAAAAGGATGGTCTAGTTTCTCTACAAGAAAAACTGCTTTCCAGAATCCTGAGGACAAAAGTTGATATTGAGGATGAATATGCAGGAGCTACTATGATAGAAAGGCGGTTATGTAAACTAAAGGTGCTTGTTGTTATTGATGATGTGGATTACATCAATCAATTAGATAAATTGGCTGGAAGTCGGGACTGGTTCGGTCCGTGGAGTAGAATTATTGTAACCACCCCAGATATTCATTTGCTAAGGGATGTTGATGATACATACAAGGCTACTGGGTTAGCTCGTGGTGAAGCTATTCAACTATTGAGTTTAAAGGCTTTCAAGAAAAGCTTCCCACCAGAAGATTATTTGGACTTGTGCCACCATATTATACGGTATGCTCAGGGGCTTCCGTTGGCTCTTGTGGTTTTAGGTTCTTTTCTATTTGGTAGAACACCCAATGAATGGGAAAGTACAATAGAGAGGCTAAACAATTCACCAAATAGACAAGTTATGGATGTGCTTCAGATTAGTTTTGATGGACTAGAGGAAAAAGACAAACAGATAGTCCTACATATTGCTTGTTTGTACAAGGGGAAGGACAGGGATCGTGTGACACAAATACTAGGCTATTGCAAGCTAGAACCTGGCATTGGTTTAAGGGTTCTTGAGGAAAAATCTCTGATTACTATTTTTAACAACAAACTGTTGATGCACGATTTGCTACAAGAAATGGGCTTGGAAATAGTTCGTCGACAGTCCCCTGAAGAGCCAAGTAAACGGAGTAGATTATGGTCTCCTGAAGATATCCACTATGTGCTGAAGAAAAATAAGAATTTTGACAAGTTAAAGTTCATCAAACTATGCCATTCTCAAAAAATTGTCCAGACCCCAGACCTCACAGGCGTTCAAAATCTTGAGACTTTAGATCTTGAAGGATGTAAGACTTTGGTAAAACTTCATCAATCCCTCGGACAACTCAAAAGGCTTATTGTATTGAATCTTAAAGATTGCGAGAATCTCAAGAATCTGCCAAGTAAGATTGAAATGGAATCTCTTGAAACTTTAATTCTTTCTAACTGCTCAAAAGTTAAAAAGATTCCTGAGTTCGCTGGAAATATGAAGCATTTGTCAAAGCTTTATCTTGATGAGACTGCCATTGAAAAACTACCTGTGTCGATTGGATGCCTGAGTGGCCTTACTTCACTGAATCTTAGCAACTGCAAAAATCTTGTTTGCCTTCCAAGCACCTTTAGTATATTGGAGTCTCTTGAAAATCTTAATCTTTCTGGATGTTTGAAACTTGGCGAACAGGATAGTGCAAGGGAGCATGGTGCAATTAGAACTGCTGTAAATCATCTTATAAAACGTGGTTGTGAAGTTGTTGGGAAATATCTGTCATGGTCCTTGCCTTCTAGATTGGTGCAAAGAGCCGATATAGAGCCAATGAGTATGAAGTTGCCTGTATCTGATCTGTGTTATTCTACAGAGCAAAATGCAAATACATCAATGAGTATCCGGTTGCCACTATCTCATCTATGTAATTTAACAGATCTAAACTTAAGTAACTGTAATCTTGGTGACACTGCATTTGCCAACTTTGCCTGTTTTCCCTCCTCAGTGGCACTAAATCTGAGTGGAAATAATTTTGTTAAGCTTCCTCCAAGCATCAGGTCCTGTTTTAAGCTTCAGAACATTAATTTGGAGAATTGCAAGACACTTCAAGAATTGTCAGGACTTCCATCAAATAGTAAATTGGATGTGAGGGCAGATGGTTGTTCTTCACTGGAAATGCTGTTTGATGTATCCAATTTCAATAGATTGGAGAAATCATATTTCAATTTCATCAATTGCTTCAAACTAAATGACAATCAGGGCTGCAGCAACATAGCGTTTGAAATGCTGAGGACATTCCGTAATCAGCGAATTGCTAGTGCCACGAAACTTTTCAAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

902

Amino Acids

102.35

Weight (kDa)

8.2

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 193 1e-48 TIR domain
TIR_2 PF13676 15 - 112 2.1e-13 TIR domain
NB-ARC PF00931 214 - 366 9.5e-15 NB-ARC domain
AAA_22 PF13401 216 - 312 1.4e-06 AAA domain
NACHT PF05729 218 - 314 3.3e-06 NACHT domain
WHD_ROQ1 PF23282 432 - 503 7.4e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 600 - 686 2.1e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_8 PF13855 601 - 657 1e-05 Leucine rich repeat
LRR_14 PF23598 611 - 679 3.7e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 125, 2111
AarI CACCTGC 1 cut(s) 95
AatII GACGTC 1 cut(s) 8
Acc36I ACCTGC 1 cut(s) 95
AccB7I CCANNNNNTGG 1 cut(s) 1212
AccI GTMKAC 3 cut(s) 25, 524, 1501
AccII CGCG 1 cut(s) 75
AciI CCGC 4 cut(s) 73, 629, 670, 874
AclWI GGATC 4 cut(s) 166, 652, 665, 1362
AcsI RAATTY 4 cut(s) 229, 533, 714, 1582
AcuI CTGAAG 6 cut(s) 40, 1256, 1533, 1569, 1589, 2420
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 1564
AfaI GTAC 3 cut(s) 373, 1222, 1337
AfiI CCNNNNNNNGG 4 cut(s) 352, 635, 1212, 1400
AflII CTTAAG 1 cut(s) 2308
AjnI CCWGG 3 cut(s) 147, 245, 1393
AjuI GAANNNNNNNTTGG 4 cut(s) 1383, 1415, 2432, 2464
Alw26I GTCTC 6 cut(s) 71, 427, 1548, 1661, 1892, 2019
AlwI GGATC 4 cut(s) 166, 652, 665, 1362
AlwNI CAGNNNCTG 1 cut(s) 2424
Ama87I CYCGRG 1 cut(s) 696
AoxI GGCC 3 cut(s) 169, 510, 1942
ApeKI GCWGC 4 cut(s) 631, 738, 2625, 2628
ApoI RAATTY 4 cut(s) 229, 533, 714, 1582
ArsI GACNNNNNNTTYG 4 cut(s) 1299, 1331, 2321, 2353
AseI ATTAAT 1 cut(s) 2445
Asp700I GAANNNNTTC 5 cut(s) 589, 1062, 1097, 1494, 2693
AspLEI GCGC 2 cut(s) 106, 345
AspS9I GGNCC 4 cut(s) 170, 965, 2151, 2421
AsuHPI GGTGA 4 cut(s) 76, 1067, 1236, 2339
AsuII TTCGAA 1 cut(s) 476
AvaI CYCGRG 1 cut(s) 696
AvaII GGWCC 3 cut(s) 965, 2151, 2421
AxyI CCTNAGG 1 cut(s) 818
BamHI GGATCC 1 cut(s) 657
BauI CACGAG 1 cut(s) 1050
BbvCI CCTCAGC 1 cut(s) 2651
BbvI GCAGC 4 cut(s) 643, 725, 2612, 2640
BccI CCATC 8 cut(s) 24, 99, 181, 445, 770, 1280, 2497, 2513
BciT130I CCWGG 3 cut(s) 149, 247, 1395
BciVI GTATCC 3 cut(s) 27, 2273, 2563
BcoDI GTCTC 6 cut(s) 71, 427, 1548, 1661, 1892, 2019
BfaI CTAG 8 cut(s) 134, 377, 782, 1292, 1373, 1388, 2163, 2679
BfmI CTRYAG 2 cut(s) 2232, 2626
BfrI CTTAAG 1 cut(s) 2308
BfuAI ACCTGC 1 cut(s) 95
BfuI GTATCC 3 cut(s) 27, 2273, 2563
BglI GCCNNNNNGGC 1 cut(s) 1941
BglII AGATCT 2 cut(s) 1675, 2299
BisI GCNGC 4 cut(s) 632, 739, 2626, 2629
BlsI GCNGC 4 cut(s) 633, 740, 2627, 2630
Bme1390I CCNGG 3 cut(s) 149, 247, 1395
Bme18I GGWCC 3 cut(s) 965, 2151, 2421
BmeT110I CYCGRG 1 cut(s) 696
BmgT120I GGNCC 4 cut(s) 170, 965, 2151, 2421
BmiI GGNNCC 2 cut(s) 330, 659
BmrFI CCNGG 3 cut(s) 149, 247, 1395
BmrI ACTGGG 1 cut(s) 1050
BmsI GCATC 3 cut(s) 1449, 1922, 2424
BmuI ACTGGG 1 cut(s) 1050
BpmI CTGGAG 1 cut(s) 170
Bpu10I CCTNAGC 3 cut(s) 1010, 1149, 2651
Bpu14I TTCGAA 1 cut(s) 476
BpuEI CTTGAG 4 cut(s) 184, 1436, 1685, 1754
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1548
BsaJI CCNNGG 3 cut(s) 661, 968, 1717
BsaWI WCCGGW 1 cut(s) 2265
Bsc4I CCNNNNNNNGG 4 cut(s) 352, 635, 1212, 1400
Bse1I ACTGG 3 cut(s) 962, 1045, 2538
Bse21I CCTNAGG 1 cut(s) 818
BseBI CCWGG 3 cut(s) 149, 247, 1395
BseDI CCNNGG 3 cut(s) 661, 968, 1717
BseLI CCNNNNNNNGG 4 cut(s) 352, 635, 1212, 1400
BseMII CTCAG 7 cut(s) 809, 1163, 1842, 1928, 2372, 2379, 2642
BseNI ACTGG 3 cut(s) 962, 1045, 2538
BseRI GAGGAG 1 cut(s) 2353
BseXI GCAGC 4 cut(s) 643, 725, 2612, 2640
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 3 cut(s) 171, 512, 1944
BsiHKCI CYCGRG 1 cut(s) 696
BsiSI CCGG 1 cut(s) 2266
BslFI GGGAC 3 cut(s) 309, 968, 1492
BslI CCNNNNNNNGG 4 cut(s) 352, 635, 1212, 1400
BsmAI GTCTC 6 cut(s) 71, 427, 1548, 1661, 1892, 2019
BsmFI GGGAC 3 cut(s) 309, 968, 1492
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 3 cut(s) 171, 512, 1944
Bso31I GGTCTC 1 cut(s) 1548
BsoBI CYCGRG 1 cut(s) 696
Bsp119I TTCGAA 1 cut(s) 476
Bsp1407I TGTACA 1 cut(s) 1335
Bsp143I GATC 8 cut(s) 158, 203, 312, 657, 1354, 1675, 2218, 2299
Bsp19I CCATGG 1 cut(s) 661
BspACI CCGC 4 cut(s) 73, 629, 670, 874
BspANI GGCC 3 cut(s) 171, 512, 1944
BspCNI CTCAG 7 cut(s) 810, 1162, 1843, 1929, 2373, 2378, 2643
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 2 cut(s) 330, 659
BspMAI CTGCAG 1 cut(s) 2630
BspMI ACCTGC 1 cut(s) 95
BspPI GGATC 4 cut(s) 166, 652, 665, 1362
BspQI GCTCTTC 1 cut(s) 1509
BspT104I TTCGAA 1 cut(s) 476
BspTI CTTAAG 1 cut(s) 2308
BspTNI GGTCTC 1 cut(s) 1548
BsrGI TGTACA 1 cut(s) 1335
BsrI ACTGG 3 cut(s) 962, 1045, 2538
BssECI CCNNGG 3 cut(s) 661, 968, 1717
BssMI GATC 8 cut(s) 158, 203, 312, 657, 1354, 1675, 2218, 2299
BssNAI GTATAC 1 cut(s) 525
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1050
BssT1I CCWWGG 1 cut(s) 661
Bst1107I GTATAC 1 cut(s) 525
Bst2BI CACGAG 1 cut(s) 1050
Bst2UI CCWGG 3 cut(s) 149, 247, 1395
Bst4CI ACNGT 4 cut(s) 1143, 1455, 1506, 2318
Bst6I CTCTTC 2 cut(s) 562, 1509
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 2308
BstAPI GCANNNNNTGC 1 cut(s) 1468
BstAUI TGTACA 1 cut(s) 1335
BstBI TTCGAA 1 cut(s) 476
BstC8I GCNNGC 2 cut(s) 668, 1385
BstDSI CCRYGG 2 cut(s) 661, 968
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 2 cut(s) 106, 345
BstKTI GATC 8 cut(s) 161, 206, 315, 660, 1357, 1678, 2221, 2302
BstMAI GTCTC 6 cut(s) 71, 427, 1548, 1661, 1892, 2019
BstMBI GATC 8 cut(s) 158, 203, 312, 657, 1354, 1675, 2218, 2299
BstMWI GCNNNNNNNGC 6 cut(s) 110, 672, 1468, 1941, 1992, 2348
BstNI CCWGG 3 cut(s) 149, 247, 1395
BstNSI RCATGY 2 cut(s) 440, 670
BstSCI CCNGG 3 cut(s) 147, 245, 1393
BstSFI CTRYAG 2 cut(s) 2232, 2626
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 4 cut(s) 643, 725, 2612, 2640
BstX2I RGATCY 3 cut(s) 657, 1675, 2299
BstXI CCANNNNNNTGG 1 cut(s) 429
BstYI RGATCY 3 cut(s) 657, 1675, 2299
BstZ17I GTATAC 1 cut(s) 525
Bsu36I CCTNAGG 1 cut(s) 818
BsuI GTATCC 3 cut(s) 27, 2273, 2563
BsuRI GGCC 3 cut(s) 171, 512, 1944
BtgI CCRYGG 2 cut(s) 661, 968
BtsI GCAGTG 1 cut(s) 2331
BtsIMutI CAGTG 4 cut(s) 1952, 2331, 2373, 2531
BveI ACCTGC 1 cut(s) 95
Cac8I GCNNGC 2 cut(s) 668, 1385
CaiI CAGNNNCTG 1 cut(s) 2424
CfoI GCGC 2 cut(s) 106, 345
Cfr13I GGNCC 4 cut(s) 170, 965, 2151, 2421
CpoI CGGWCCG 1 cut(s) 965
Csp6I GTAC 3 cut(s) 372, 1221, 1336
CspI CGGWCCG 1 cut(s) 965
CviAII CATG 9 cut(s) 338, 392, 437, 542, 662, 667, 707, 2078, 2148
CviQI GTAC 3 cut(s) 372, 1221, 1336
DpnI GATC 8 cut(s) 160, 205, 314, 659, 1356, 1677, 2220, 2301
DpnII GATC 8 cut(s) 158, 203, 312, 657, 1354, 1675, 2218, 2299
DraI TTTAAA 1 cut(s) 1080
DraIII CACNNNGTG 1 cut(s) 1564
Eam1104I CTCTTC 2 cut(s) 562, 1509
EarI CTCTTC 2 cut(s) 562, 1509
Eco130I CCWWGG 1 cut(s) 661
Eco31I GGTCTC 1 cut(s) 1548
Eco32I GATATC 1 cut(s) 1555
Eco47I GGWCC 3 cut(s) 965, 2151, 2421
Eco57I CTGAAG 6 cut(s) 40, 1256, 1533, 1569, 1589, 2420
Eco81I CCTNAGG 1 cut(s) 818
Eco88I CYCGRG 1 cut(s) 696
EcoO109I RGGNCCY 1 cut(s) 2421
EcoRII CCWGG 3 cut(s) 147, 245, 1393
EcoRV GATATC 1 cut(s) 1555
EcoT14I CCWWGG 1 cut(s) 661
ErhI CCWWGG 1 cut(s) 661
FaeI CATG 9 cut(s) 341, 395, 440, 545, 665, 670, 710, 2081, 2151
FalI AAGNNNNNCTT 2 cut(s) 1082, 1114
FaqI GGGAC 3 cut(s) 309, 968, 1492
FatI CATG 9 cut(s) 337, 391, 436, 541, 661, 666, 706, 2077, 2147
FauI CCCGC 2 cut(s) 80, 663
FauNDI CATATG 1 cut(s) 623
FblI GTMKAC 3 cut(s) 25, 524, 1501
Fnu4HI GCNGC 4 cut(s) 632, 739, 2626, 2629
Fsp4HI GCNGC 4 cut(s) 632, 739, 2626, 2629
FspBI CTAG 8 cut(s) 134, 377, 782, 1292, 1373, 1388, 2163, 2679
GlaI GCGC 2 cut(s) 105, 344
GluI GCNGC 4 cut(s) 632, 739, 2626, 2629
GsuI CTGGAG 1 cut(s) 170
HaeIII GGCC 3 cut(s) 171, 512, 1944
HapII CCGG 1 cut(s) 2266
HhaI GCGC 2 cut(s) 106, 345
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 9 cut(s) 341, 395, 440, 545, 665, 670, 710, 2081, 2151
Hin6I GCGC 2 cut(s) 104, 343
HinP1I GCGC 2 cut(s) 104, 343
HincII GTYRAC 4 cut(s) 9, 26, 568, 1502
HindII GTYRAC 4 cut(s) 9, 26, 568, 1502
HindIII AAGCTT 5 cut(s) 52, 1096, 1883, 2402, 2432
HpaII CCGG 1 cut(s) 2266
HphI GGTGA 4 cut(s) 76, 1067, 1236, 2339
Hpy166II GTNNAC 8 cut(s) 9, 26, 374, 525, 568, 884, 1502, 1526
Hpy188I TCNGA 9 cut(s) 59, 226, 769, 1275, 1431, 1721, 2218, 2382, 2439
Hpy8I GTNNAC 8 cut(s) 9, 26, 374, 525, 568, 884, 1502, 1526
Hpy99I CGWCG 3 cut(s) 27, 30, 1503
HpyAV CCTTC 4 cut(s) 1339, 1676, 1997, 2169
HpyCH4III ACNGT 4 cut(s) 1143, 1455, 1506, 2318
HpyCH4IV ACGT 2 cut(s) 5, 2116
HpyF10VI GCNNNNNNNGC 6 cut(s) 110, 672, 1468, 1941, 1992, 2348
HpySE526I ACGT 2 cut(s) 5, 2116
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 9 cut(s) 341, 395, 440, 545, 665, 670, 710, 2081, 2151
HspAI GCGC 2 cut(s) 104, 343
Kzo9I GATC 8 cut(s) 158, 203, 312, 657, 1354, 1675, 2218, 2299
LguI GCTCTTC 1 cut(s) 1509
LmnI GCTCC 4 cut(s) 334, 372, 854, 2074
Lsp1109I GCAGC 4 cut(s) 643, 725, 2612, 2640
LweI GCATC 3 cut(s) 1449, 1922, 2424
MaeI CTAG 8 cut(s) 134, 377, 782, 1292, 1373, 1388, 2163, 2679
MaeII ACGT 2 cut(s) 5, 2116
MaeIII GTNAC 4 cut(s) 985, 1360, 2312, 2327
MalI GATC 8 cut(s) 160, 205, 314, 659, 1356, 1677, 2220, 2301
MboI GATC 8 cut(s) 158, 203, 312, 657, 1354, 1675, 2218, 2299
MfeI CAATTG 1 cut(s) 2596
MflI RGATCY 3 cut(s) 657, 1675, 2299
MlyI GAGTC 2 cut(s) 191, 2021
MroXI GAANNNNTTC 5 cut(s) 589, 1062, 1097, 1494, 2693
MslI CAYNNNNRTG 2 cut(s) 247, 584
MspCI CTTAAG 1 cut(s) 2308
MspI CCGG 1 cut(s) 2266
MspR9I CCNGG 3 cut(s) 149, 247, 1395
MunI CAATTG 1 cut(s) 2596
Mva1269I GAATGC 1 cut(s) 741
MvaI CCWGG 3 cut(s) 149, 247, 1395
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 6 cut(s) 110, 672, 1468, 1941, 1992, 2348
NcoI CCATGG 1 cut(s) 661
NdeI CATATG 1 cut(s) 623
NdeII GATC 8 cut(s) 158, 203, 312, 657, 1354, 1675, 2218, 2299
NlaIII CATG 9 cut(s) 341, 395, 440, 545, 665, 670, 710, 2081, 2151
NlaIV GGNNCC 2 cut(s) 330, 659
NmuCI GTSAC 2 cut(s) 1360, 2327
NspI RCATGY 2 cut(s) 440, 670
NspV TTCGAA 1 cut(s) 476
PaeI GCATGC 1 cut(s) 670
PaeR7I CTCGAG 1 cut(s) 696
PaqCI CACCTGC 1 cut(s) 95
PciSI GCTCTTC 1 cut(s) 1509
PctI GAATGC 1 cut(s) 741
PdmI GAANNNNTTC 5 cut(s) 589, 1062, 1097, 1494, 2693
PfeI GAWTC 9 cut(s) 473, 590, 813, 1747, 1765, 1774, 1799, 1846, 1957
PflMI CCANNNNNTGG 1 cut(s) 1212
PkrI GCNGC 4 cut(s) 633, 740, 2627, 2630
PleI GAGTC 2 cut(s) 191, 2020
PpsI GAGTC 2 cut(s) 191, 2020
PpuMI RGGWCCY 1 cut(s) 2421
PshBI ATTAAT 1 cut(s) 2445
PsiI TTATAA 2 cut(s) 125, 2111
Psp5II RGGWCCY 1 cut(s) 2421
Psp6I CCWGG 3 cut(s) 147, 245, 1393
PspGI CCWGG 3 cut(s) 147, 245, 1393
PspN4I GGNNCC 2 cut(s) 330, 659
PspPI GGNCC 4 cut(s) 170, 965, 2151, 2421
PspPPI RGGWCCY 1 cut(s) 2421
PspXI VCTCGAGB 1 cut(s) 696
PstI CTGCAG 1 cut(s) 2630
PstNI CAGNNNCTG 1 cut(s) 2424
PsuI RGATCY 3 cut(s) 657, 1675, 2299
RsaI GTAC 3 cut(s) 373, 1222, 1337
RsaNI GTAC 3 cut(s) 372, 1221, 1336
RseI CAYNNNNRTG 2 cut(s) 247, 584
Rsr2I CGGWCCG 1 cut(s) 965
RsrII CGGWCCG 1 cut(s) 965
SalI GTCGAC 2 cut(s) 24, 1500
SapI GCTCTTC 1 cut(s) 1509
SatI GCNGC 4 cut(s) 632, 739, 2626, 2629
Sau3AI GATC 8 cut(s) 158, 203, 312, 657, 1354, 1675, 2218, 2299
Sau96I GGNCC 4 cut(s) 170, 965, 2151, 2421
SchI GAGTC 2 cut(s) 191, 2021
ScrFI CCNGG 3 cut(s) 149, 247, 1395
SfaNI GCATC 3 cut(s) 1449, 1922, 2424
SfcI CTRYAG 2 cut(s) 2232, 2626
Sfr274I CTCGAG 1 cut(s) 696
SfuI TTCGAA 1 cut(s) 476
SgrDI CGTCGACG 1 cut(s) 24
SinI GGWCC 3 cut(s) 965, 2151, 2421
SlaI CTCGAG 1 cut(s) 696
SmiMI CAYNNNNRTG 2 cut(s) 247, 584
SmlI CTYRAG 6 cut(s) 199, 696, 1415, 1664, 1769, 2308
SmoI CTYRAG 6 cut(s) 199, 696, 1415, 1664, 1769, 2308
SphI GCATGC 1 cut(s) 670
SsiI CCGC 4 cut(s) 73, 629, 670, 874
SspMI CTAG 8 cut(s) 134, 377, 782, 1292, 1373, 1388, 2163, 2679
StyD4I CCNGG 3 cut(s) 147, 245, 1393
StyI CCWWGG 1 cut(s) 661
TaaI ACNGT 4 cut(s) 1143, 1455, 1506, 2318
TaiI ACGT 2 cut(s) 8, 2119
TaqI TCGA 6 cut(s) 25, 266, 476, 697, 1501, 1925
TaqII GACCGA 1 cut(s) 953
TatI WGTACW 2 cut(s) 1220, 1335
TfiI GAWTC 9 cut(s) 473, 590, 813, 1747, 1765, 1774, 1799, 1846, 1957
TscAI CASTG 4 cut(s) 1959, 2338, 2373, 2538
TseFI GTSAC 2 cut(s) 1360, 2327
TseI GCWGC 4 cut(s) 631, 738, 2625, 2628
Tsp45I GTSAC 2 cut(s) 1360, 2327
TspGWI ACGGA 6 cut(s) 359, 510, 957, 1149, 1543, 2651
TspRI CASTG 4 cut(s) 1959, 2338, 2373, 2538
Van91I CCANNNNNTGG 1 cut(s) 1212
Vha464I CTTAAG 1 cut(s) 2308
VpaK11BI GGWCC 3 cut(s) 965, 2151, 2421
VspI ATTAAT 1 cut(s) 2445
XapI RAATTY 4 cut(s) 229, 533, 714, 1582
XbaI TCTAGA 2 cut(s) 133, 2162
XceI RCATGY 2 cut(s) 440, 670
XhoI CTCGAG 1 cut(s) 696
XmiI GTMKAC 3 cut(s) 25, 524, 1501
XmnI GAANNNNTTC 5 cut(s) 589, 1062, 1097, 1494, 2693
XspI CTAG 8 cut(s) 134, 377, 782, 1292, 1373, 1388, 2163, 2679
ZraI GACGTC 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.