Rmu_sc0000749.1_g000041

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000749.1
OG0000105
Physical Location & Seq
Forward (+)
158318 .. 162515
4198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000749.1_g000041.1.cds

Sequence Viewer

Length: 3372 bp
atgttttggtattcgatcggaaaccggttgagctccgacgaacgtgaatccggtcactccaggtgcgatccttctggaatgatgatcaccaggccgttccgccgtgcaaggtcaccacagacaggtgcgcaaggaccatacgaagccaatggaagaagtttggtgaagattggtggccggaggaggaagatatggcttggagaaggaaaggtgcgatttccggtcgggggaacgaagctgcagctcgatttccatttttggaagtttcggaaattattttcggcaaaactacatcaaacagaactaattgaggaaattgttaatcatatactaaagaagtccgtctatacatcttcaagtgttgacaagggcttgataggaatggagtcccgcattgatgatttattaagcaattacatatttgcgcagcttggtggggtgcgttttattgggatccatggcatgcggggcataggtaagacaacagttgctcgagctatctatgatgaaatttgtcagggtttttaccgaagctgctttctttccaatgttagagaaatgtctaaaaacaatggccttgtttctctacaagaaaaacttctttccagaatcctgatggcaaaagttgaaaatatagaggatgaatacacaggagctgctatgatagaaaggcggttgtgtagaataaaggtgcttgttgttattgatgatgtggatcagttgtcacaattagagaaattggctggaagtcgcaactggtttggtccagggagtagaattatcataaccaccacagatattcagttgttaaaggcacatgatgttgatgctacgtacaacgcttatgggttaaactgtgatgaaacactgcaacttttgagtttgaaggcttttaagaagtgttccccaccagaagattatttgcaactgtgctaccatattttagggtatgctcaggggcttccgttggctcttgtggttttaggttcttttctatttggtagaagcactgatgaatgggcaagtgcaatagataggctaaagaacacaccagataaacgcattattgaggtgcttcggattagttttgatggcctggatgaaaaagacaaagaaatattcctacatattgcttgcttttacaaggggaaggataaggatcgagtgacacaaatactagactattgcaagctaaaccctgtcattggtttaagtgttcttgctgatagatgtctcatcactatctccaacaacgaactgtcgatgcatgatttgctacaagaaatgggctgggaaattgttcgtgaacagtctcctgaagagccaggcaaacgtagtagattatggtctcatgaagacatcaacactgtgctgaagagaaataagggaacagattcaatccaaggcatggtaatggagttgactgaattacaagtggctcattggatgccaaaagccttttcaaatttgtctcaacttattctactccatattcataatgtggaccttcctgaaggcctcacttgtctctctaattccttgagactcctcgaatggacggggtatcccttaagatctctcccaaaaaaatttgaagcagatgaacttattgaacttaacttgtgccaaagcaacattaaacagctttggaagggaacaaagaattttgacaagttgaagttcatcaaactctgccattctcaaaacattgtggagaccccagatctcggaggtgttcagaatcttgagactttagatcttgaaggatgtgagaatttggtaagaatccatagatcccttggatttcacaaaaagcttattgtcctgaatcttaaagactgcaaaagtctcaggagtctgccaagtcaaataaaaatggaatctcttgaaacattaattctttctaactgctcaaaagttaagaagattcccgagtttgtcggaaatatggaacgtttgttggtgctttgtcttgatgagactgccattgaggaactgcctgtttcaattgaacggctgagtggccttgtgtcattgaatctaagcaactgcaaaaatcttgtttgtcttccaagcaccatcaataaattgaagtctgttgttaatctttctggatgcttgaaactccgcaaacatcaggtaaatgtgggggagatggaatgttttgaggaaactgatgtgaatagtgggtctgcaatagaaatgtcatctacccatgatcgcataaaatatgtgagaggttcaatctttcatggatgcaaagtagtttggcgaactttaaataatttcttgccttctggattggtgcaaaaagtgaacacagagccaatgaatttccgcttgcctatatctggtctgtgtaatttaacatatctaaacctgagtaattgcaatcttggtgaaggagcatttgcctgcaaatttggttactttccctcattggtgaccttgaatctaagtggaaacaattttgttcgccttccttcaggcattggattgctttctaagcttgagaactttaacttggagaattgcaagagacttcaagagttgtcagatcttccatcaaatagtagactagatttaagggcagatggttgtacttcacttaaatacttgtttgatgcatcaaacttgaacagattaaacaaatcatatttcaatttcatcaattgcttcaatctaaatagcaatcaaggatgcagtaacatagcatttgaaatgctgaagacattcatgtatcagggaatctctaataagagggaaacttttcaaattgtaattcctggaagtaatattcatgagtggttcagccatcagagtgttgggtgttcattaagtgtgagacctgcgtattggaataacagtcggtttttaggatttgctttgtgtgctgtttttgtactccatgagcaccatcaggtggatgagctttatgtagatgaattcaagacttttaatgcaacacatcatcttgtatgttgcatgaagctcgatggcagagaattgaaagtgtatggcaaccagcctgcatttcgctttagtgaagaattttgccaggttgaatcagatcacctgtggctattctatgtatctcgtgataaatactttggtacggagtggtggcataactgttatagtcagcttgagttcttatttgaaacccgagggccgggtctgaaggtaaagaagtgtgcagtccgtctgatatatgagcaagatgtgcaagagttgaaccaaagaatcactcaaccaagcagtaggatgtctccttatgaggatatattgagtggttttgacattccagttgcgagggaaaccagtggcactggtagcagaatttgcacgcttgaagaattgttggactggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1123

Amino Acids

127.9

Weight (kDa)

8.09

Isoelectric Point (pI)

45.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 129, 426
Acc36I ACCTGC 1 cut(s) 2857
AccB7I CCANNNNNTGG 2 cut(s) 1408, 2923
AccI GTMKAC 1 cut(s) 2575
AciI CCGC 6 cut(s) 100, 391, 466, 673, 2128, 2338
AclI AACGTT 1 cut(s) 1954
AclWI GGATC 6 cut(s) 62, 448, 461, 723, 1167, 1788
AcoI YGGCCR 1 cut(s) 175
AcuI CTGAAG 6 cut(s) 1338, 1394, 1533, 2469, 2747, 3200
AfaI GTAC 4 cut(s) 836, 2602, 2904, 3115
AfiI CCNNNNNNNGG 8 cut(s) 122, 227, 1205, 1408, 1727, 2351, 2923, 3172
AflII CTTAAG 1 cut(s) 1570
AgeI ACCGGT 1 cut(s) 24
AjnI CCWGG 7 cut(s) 59, 89, 766, 1095, 1324, 2785, 3057
AjuI GAANNNNNNNTTGG 4 cut(s) 1444, 1476, 2507, 2539
Alw21I GWGCWC 2 cut(s) 35, 2916
AlwI GGATC 6 cut(s) 62, 448, 461, 723, 1167, 1788
AlwNI CAGNNNCTG 1 cut(s) 656
Ama87I CYCGRG 3 cut(s) 492, 1931, 3165
AoxI GGCC 7 cut(s) 92, 175, 574, 1093, 1516, 2023, 3170
ApeKI GCWGC 5 cut(s) 238, 241, 427, 534, 656
AseI ATTAAT 1 cut(s) 1895
AsiGI ACCGGT 1 cut(s) 24
Asp700I GAANNNNTTC 5 cut(s) 597, 1299, 1393, 2732, 2769
AspLEI GCGC 2 cut(s) 130, 427
AspS9I GGNCC 4 cut(s) 134, 764, 1504, 3170
AsuC2I CCSGG 1 cut(s) 3174
AsuHPI GGTGA 6 cut(s) 79, 105, 175, 2411, 2455, 3065
AvaI CYCGRG 3 cut(s) 492, 1931, 3165
AvaII GGWCC 3 cut(s) 134, 764, 1504
BamHI GGATCC 1 cut(s) 453
BanII GRGCYC 1 cut(s) 35
BauI CACGAG 1 cut(s) 3096
BbsI GAAGAC 3 cut(s) 1362, 2060, 2735
Bbv12I GWGCWC 2 cut(s) 35, 2916
BbvI GCAGC 5 cut(s) 225, 253, 439, 521, 643
BccI CCATC 9 cut(s) 610, 1085, 2087, 2149, 2572, 2588, 2823, 2925, 2990
BceAI ACGGC 3 cut(s) 79, 87, 2030
BcgI CGANNNNNNTGC 2 cut(s) 2974, 3008
BciT130I CCWGG 7 cut(s) 61, 91, 768, 1097, 1326, 2787, 3059
BciVI GTATCC 1 cut(s) 1575
BclI TGATCA 1 cut(s) 84
BcnI CCSGG 1 cut(s) 3174
BfaI CTAG 2 cut(s) 1178, 2579
BfmI CTRYAG 1 cut(s) 239
BfrI CTTAAG 1 cut(s) 1570
BfuAI ACCTGC 1 cut(s) 2857
BfuI GTATCC 1 cut(s) 1575
BglII AGATCT 4 cut(s) 1574, 1723, 1756, 2557
BisI GCNGC 5 cut(s) 239, 242, 428, 535, 657
BlsI GCNGC 5 cut(s) 240, 243, 429, 536, 658
Bme1390I CCNGG 8 cut(s) 61, 91, 768, 1097, 1326, 2787, 3059, 3174
Bme18I GGWCC 3 cut(s) 134, 764, 1504
BmeT110I CYCGRG 3 cut(s) 492, 1931, 3165
BmgT120I GGNCC 4 cut(s) 134, 764, 1504, 3170
BmiI GGNNCC 1 cut(s) 455
BmrFI CCNGG 8 cut(s) 61, 91, 768, 1097, 1326, 2787, 3059, 3174
BmsI GCATC 8 cut(s) 817, 1254, 1437, 2105, 2246, 2614, 2636, 2690
BpiI GAAGAC 3 cut(s) 1362, 2060, 2735
BpmI CTGGAG 1 cut(s) 43
Bpu10I CCTNAGC 1 cut(s) 954
BpuEI CTTGAG 4 cut(s) 1561, 1766, 2531, 3167
BpuMI CCSGG 1 cut(s) 3174
BsaAI YACGTR 1 cut(s) 834
BsaBI GATNNNNATC 2 cut(s) 714, 1158
BsaI GGTCTC 3 cut(s) 1353, 1709, 2839
BsaJI CCNNGG 5 cut(s) 457, 767, 1402, 1798, 3166
BsaWI WCCGGW 3 cut(s) 24, 50, 220
Bsc4I CCNNNNNNNGG 8 cut(s) 122, 227, 1205, 1408, 1727, 2351, 2923, 3172
Bse118I RCCGGY 1 cut(s) 24
Bse1I ACTGG 5 cut(s) 761, 3305, 3321, 3334, 3371
Bse8I GATNNNNATC 2 cut(s) 714, 1158
BseBI CCWGG 7 cut(s) 61, 91, 768, 1097, 1326, 2787, 3059
BseDI CCNNGG 5 cut(s) 457, 767, 1402, 1798, 3166
BseGI GGATG 9 cut(s) 646, 1105, 1452, 1772, 2120, 2261, 2705, 2932, 3270
BseJI GATNNNNATC 2 cut(s) 714, 1158
BseLI CCNNNNNNNGG 8 cut(s) 122, 227, 1205, 1408, 1727, 2351, 2923, 3172
BseMII CTCAG 4 cut(s) 968, 1864, 2009, 2372
BseNI ACTGG 5 cut(s) 761, 3305, 3321, 3334, 3371
BseRI GAGGAG 2 cut(s) 196, 1538
BseXI GCAGC 5 cut(s) 225, 253, 439, 521, 643
BseYI CCCAGC 1 cut(s) 1290
BsgI GTGCAG 1 cut(s) 3216
Bsh1285I CGRYCG 2 cut(s) 18, 225
BshFI GGCC 7 cut(s) 94, 177, 576, 1095, 1518, 2025, 3172
BshTI ACCGGT 1 cut(s) 24
BsiEI CGRYCG 2 cut(s) 18, 225
BsiHKAI GWGCWC 2 cut(s) 35, 2916
BsiHKCI CYCGRG 3 cut(s) 492, 1931, 3165
BsiSI CCGG 5 cut(s) 25, 51, 178, 221, 3173
BslFI GGGAC 1 cut(s) 373
BslI CCNNNNNNNGG 8 cut(s) 122, 227, 1205, 1408, 1727, 2351, 2923, 3172
BsmFI GGGAC 1 cut(s) 373
BsnI GGCC 7 cut(s) 94, 177, 576, 1095, 1518, 2025, 3172
Bso31I GGTCTC 3 cut(s) 1353, 1709, 2839
BsoBI CYCGRG 3 cut(s) 492, 1931, 3165
Bsp1286I GDGCHC 2 cut(s) 35, 2916
Bsp19I CCATGG 1 cut(s) 457
BspACI CCGC 6 cut(s) 100, 391, 466, 673, 2128, 2338
BspANI GGCC 7 cut(s) 94, 177, 576, 1095, 1518, 2025, 3172
BspCNI CTCAG 4 cut(s) 967, 1863, 2010, 2373
BspHI TCATGA 2 cut(s) 1351, 2800
BspLI GGNNCC 1 cut(s) 455
BspMAI CTGCAG 1 cut(s) 243
BspMI ACCTGC 1 cut(s) 2857
BspPI GGATC 6 cut(s) 62, 448, 461, 723, 1167, 1788
BspQI GCTCTTC 1 cut(s) 1314
BspTI CTTAAG 1 cut(s) 1570
BspTNI GGTCTC 3 cut(s) 1353, 1709, 2839
BsrFI RCCGGY 1 cut(s) 24
BsrI ACTGG 5 cut(s) 761, 3305, 3321, 3334, 3371
BssAI RCCGGY 1 cut(s) 24
BssECI CCNNGG 5 cut(s) 457, 767, 1402, 1798, 3166
BssSI CACGAG 1 cut(s) 3096
BssT1I CCWWGG 3 cut(s) 457, 1402, 1798
Bst2BI CACGAG 1 cut(s) 3096
Bst2UI CCWGG 7 cut(s) 61, 91, 768, 1097, 1326, 2787, 3059
Bst4CI ACNGT 8 cut(s) 487, 857, 930, 1260, 1311, 1369, 2867, 3134
Bst6I CTCTTC 2 cut(s) 1314, 1370
BstAFI CTTAAG 1 cut(s) 1570
BstAPI GCANNNNNTGC 3 cut(s) 1273, 3223, 3342
BstBAI YACGTR 1 cut(s) 834
BstC8I GCNNGC 7 cut(s) 464, 1135, 1190, 2342, 2416, 3030, 3347
BstDEI CTNAG 7 cut(s) 954, 1850, 2018, 2042, 2381, 2456, 2505
BstDSI CCRYGG 1 cut(s) 457
BstEII GGTNACC 2 cut(s) 111, 2443
BstF5I GGATG 9 cut(s) 646, 1105, 1452, 1772, 2120, 2261, 2705, 2932, 3270
BstHHI GCGC 2 cut(s) 130, 427
BstMCI CGRYCG 2 cut(s) 18, 225
BstMWI GCNNNNNNNGC 7 cut(s) 468, 1273, 2506, 2891, 3223, 3333, 3342
BstNI CCWGG 7 cut(s) 61, 91, 768, 1097, 1326, 2787, 3059
BstNSI RCATGY 1 cut(s) 466
BstPI GGTNACC 2 cut(s) 111, 2443
BstSCI CCNGG 8 cut(s) 59, 89, 766, 1095, 1324, 2785, 3057, 3172
BstSFI CTRYAG 1 cut(s) 239
BstSNI TACGTA 1 cut(s) 834
BstV1I GCAGC 5 cut(s) 225, 253, 439, 521, 643
BstV2I GAAGAC 3 cut(s) 1362, 2060, 2735
BstX2I RGATCY 6 cut(s) 453, 1574, 1723, 1756, 1793, 2557
BstYI RGATCY 6 cut(s) 453, 1574, 1723, 1756, 1793, 2557
BsuI GTATCC 1 cut(s) 1575
BsuRI GGCC 7 cut(s) 94, 177, 576, 1095, 1518, 2025, 3172
BtgI CCRYGG 1 cut(s) 457
BtsCI GGATG 9 cut(s) 646, 1105, 1452, 1772, 2120, 2261, 2705, 2932, 3270
BtsI GCAGTG 1 cut(s) 866
BtsIMutI CAGTG 5 cut(s) 866, 1008, 1365, 3327, 3328
BveI ACCTGC 1 cut(s) 2857
Cac8I GCNNGC 7 cut(s) 464, 1135, 1190, 2342, 2416, 3030, 3347
CaiI CAGNNNCTG 1 cut(s) 656
CciI TCATGA 2 cut(s) 1351, 2800
CfoI GCGC 2 cut(s) 130, 427
Cfr10I RCCGGY 1 cut(s) 24
Cfr13I GGNCC 4 cut(s) 134, 764, 1504, 3170
Csp6I GTAC 4 cut(s) 835, 2601, 2903, 3114
CspAI ACCGGT 1 cut(s) 24
CviQI GTAC 4 cut(s) 835, 2601, 2903, 3114
DdeI CTNAG 7 cut(s) 954, 1850, 2018, 2042, 2381, 2456, 2505
DraI TTTAAA 1 cut(s) 2280
EaeI YGGCCR 1 cut(s) 175
Eam1104I CTCTTC 2 cut(s) 1314, 1370
EarI CTCTTC 2 cut(s) 1314, 1370
EciI GGCGGA 1 cut(s) 89
Ecl136II GAGCTC 1 cut(s) 33
Eco105I TACGTA 1 cut(s) 834
Eco130I CCWWGG 3 cut(s) 457, 1402, 1798
Eco147I AGGCCT 1 cut(s) 1518
Eco24I GRGCYC 1 cut(s) 35
Eco31I GGTCTC 3 cut(s) 1353, 1709, 2839
Eco47I GGWCC 3 cut(s) 134, 764, 1504
Eco53kI GAGCTC 1 cut(s) 33
Eco57I CTGAAG 6 cut(s) 1338, 1394, 1533, 2469, 2747, 3200
Eco88I CYCGRG 3 cut(s) 492, 1931, 3165
Eco91I GGTNACC 2 cut(s) 111, 2443
EcoICRI GAGCTC 1 cut(s) 33
EcoO65I GGTNACC 2 cut(s) 111, 2443
EcoRI GAATTC 1 cut(s) 2945
EcoRII CCWGG 7 cut(s) 59, 89, 766, 1095, 1324, 2785, 3057
EcoT14I CCWWGG 3 cut(s) 457, 1402, 1798
EcoT22I ATGCAT 2 cut(s) 1269, 2629
EcoT38I GRGCYC 1 cut(s) 35
ErhI CCWWGG 3 cut(s) 457, 1402, 1798
FalI AAGNNNNNCTT 2 cut(s) 582, 614
FaqI GGGAC 1 cut(s) 373
FauI CCCGC 2 cut(s) 398, 459
FbaI TGATCA 1 cut(s) 84
FblI GTMKAC 1 cut(s) 2575
Fnu4HI GCNGC 5 cut(s) 239, 242, 428, 535, 657
FokI GGATG 9 cut(s) 653, 1112, 1459, 1779, 2127, 2268, 2712, 2939, 3277
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 5 cut(s) 239, 242, 428, 535, 657
FspBI CTAG 2 cut(s) 1178, 2579
FspI TGCGCA 2 cut(s) 129, 426
GlaI GCGC 2 cut(s) 129, 426
GluI GCNGC 5 cut(s) 239, 242, 428, 535, 657
GsaI CCCAGC 1 cut(s) 1294
GsuI CTGGAG 1 cut(s) 43
HaeIII GGCC 7 cut(s) 94, 177, 576, 1095, 1518, 2025, 3172
HapII CCGG 5 cut(s) 25, 51, 178, 221, 3173
HhaI GCGC 2 cut(s) 130, 427
Hin6I GCGC 2 cut(s) 128, 425
HinP1I GCGC 2 cut(s) 128, 425
HincII GTYRAC 2 cut(s) 364, 1422
HindII GTYRAC 2 cut(s) 364, 1422
HindIII AAGCTT 2 cut(s) 1814, 2507
HpaII CCGG 5 cut(s) 25, 51, 178, 221, 3173
HphI GGTGA 6 cut(s) 79, 105, 175, 2411, 2455, 3065
Hpy166II GTNNAC 6 cut(s) 364, 1307, 1422, 1504, 2317, 2576
Hpy8I GTNNAC 6 cut(s) 364, 1307, 1422, 1504, 2317, 2576
Hpy99I CGWCG 1 cut(s) 41
HpyCH4III ACNGT 8 cut(s) 487, 857, 930, 1260, 1311, 1369, 2867, 3134
HpyCH4IV ACGT 4 cut(s) 43, 833, 1333, 1954
HpyF10VI GCNNNNNNNGC 7 cut(s) 468, 1273, 2506, 2891, 3223, 3333, 3342
HpyF3I CTNAG 7 cut(s) 954, 1850, 2018, 2042, 2381, 2456, 2505
HpySE526I ACGT 4 cut(s) 43, 833, 1333, 1954
HspAI GCGC 2 cut(s) 128, 425
Ksp22I TGATCA 1 cut(s) 84
LguI GCTCTTC 1 cut(s) 1314
LmnI GCTCC 3 cut(s) 38, 653, 2405
Lsp1109I GCAGC 5 cut(s) 225, 253, 439, 521, 643
LweI GCATC 8 cut(s) 817, 1254, 1437, 2105, 2246, 2614, 2636, 2690
MaeI CTAG 2 cut(s) 1178, 2579
MaeII ACGT 4 cut(s) 43, 833, 1333, 1954
MaeIII GTNAC 7 cut(s) 53, 111, 723, 1165, 2426, 2443, 2705
MfeI CAATTG 2 cut(s) 2007, 2671
MflI RGATCY 6 cut(s) 453, 1574, 1723, 1756, 1793, 2557
MhlI GDGCHC 2 cut(s) 35, 2916
MlyI GAGTC 3 cut(s) 395, 1539, 1864
MmeI TCCRAC 4 cut(s) 60, 1272, 1921, 3342
Mph1103I ATGCAT 2 cut(s) 1269, 2629
MroXI GAANNNNTTC 5 cut(s) 597, 1299, 1393, 2732, 2769
MslI CAYNNNNRTG 2 cut(s) 1412, 2820
MspCI CTTAAG 1 cut(s) 1570
MspI CCGG 5 cut(s) 25, 51, 178, 221, 3173
MspR9I CCNGG 8 cut(s) 61, 91, 768, 1097, 1326, 2787, 3059, 3174
MunI CAATTG 2 cut(s) 2007, 2671
MvaI CCWGG 7 cut(s) 61, 91, 768, 1097, 1326, 2787, 3059
MwoI GCNNNNNNNGC 7 cut(s) 468, 1273, 2506, 2891, 3223, 3333, 3342
NciI CCSGG 1 cut(s) 3174
NcoI CCATGG 1 cut(s) 457
NlaIV GGNNCC 1 cut(s) 455
NmuCI GTSAC 5 cut(s) 53, 111, 723, 1165, 2443
NsbI TGCGCA 2 cut(s) 129, 426
NsiI ATGCAT 2 cut(s) 1269, 2629
NspI RCATGY 1 cut(s) 466
PaeI GCATGC 1 cut(s) 466
PaeR7I CTCGAG 1 cut(s) 492
PagI TCATGA 2 cut(s) 1351, 2800
PceI AGGCCT 1 cut(s) 1518
PciSI GCTCTTC 1 cut(s) 1314
PdmI GAANNNNTTC 5 cut(s) 597, 1299, 1393, 2732, 2769
PflMI CCANNNNNTGG 2 cut(s) 1408, 2923
PfoI TCCNGGA 1 cut(s) 2785
PinAI ACCGGT 1 cut(s) 24
PkrI GCNGC 5 cut(s) 240, 243, 429, 536, 658
Ple19I CGATCG 1 cut(s) 18
PleI GAGTC 3 cut(s) 394, 1539, 1863
PpsI GAGTC 3 cut(s) 394, 1539, 1863
Ppu21I YACGTR 1 cut(s) 834
PshBI ATTAAT 1 cut(s) 1895
Psp124BI GAGCTC 1 cut(s) 35
Psp1406I AACGTT 1 cut(s) 1954
Psp6I CCWGG 7 cut(s) 59, 89, 766, 1095, 1324, 2785, 3057
PspEI GGTNACC 2 cut(s) 111, 2443
PspFI CCCAGC 1 cut(s) 1290
PspGI CCWGG 7 cut(s) 59, 89, 766, 1095, 1324, 2785, 3057
PspN4I GGNNCC 1 cut(s) 455
PspPI GGNCC 4 cut(s) 134, 764, 1504, 3170
PspXI VCTCGAGB 1 cut(s) 492
PstI CTGCAG 1 cut(s) 243
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 6 cut(s) 453, 1574, 1723, 1756, 1793, 2557
PvuI CGATCG 1 cut(s) 18
RsaI GTAC 4 cut(s) 836, 2602, 2904, 3115
RsaNI GTAC 4 cut(s) 835, 2601, 2903, 3114
RseI CAYNNNNRTG 2 cut(s) 1412, 2820
SacI GAGCTC 1 cut(s) 35
SapI GCTCTTC 1 cut(s) 1314
SatI GCNGC 5 cut(s) 239, 242, 428, 535, 657
Sau96I GGNCC 4 cut(s) 134, 764, 1504, 3170
SchI GAGTC 3 cut(s) 395, 1539, 1864
ScrFI CCNGG 8 cut(s) 61, 91, 768, 1097, 1326, 2787, 3059, 3174
SduI GDGCHC 2 cut(s) 35, 2916
SfaNI GCATC 8 cut(s) 817, 1254, 1437, 2105, 2246, 2614, 2636, 2690
SfcI CTRYAG 1 cut(s) 239
Sfr274I CTCGAG 1 cut(s) 492
SinI GGWCC 3 cut(s) 134, 764, 1504
SlaI CTCGAG 1 cut(s) 492
SmiMI CAYNNNNRTG 2 cut(s) 1412, 2820
SmlI CTYRAG 6 cut(s) 492, 1540, 1570, 1745, 2510, 3146
SmoI CTYRAG 6 cut(s) 492, 1540, 1570, 1745, 2510, 3146
SnaBI TACGTA 1 cut(s) 834
SphI GCATGC 1 cut(s) 466
SseBI AGGCCT 1 cut(s) 1518
SsiI CCGC 6 cut(s) 100, 391, 466, 673, 2128, 2338
SspI AATATT 2 cut(s) 1119, 2797
SspMI CTAG 2 cut(s) 1178, 2579
SstI GAGCTC 1 cut(s) 35
StuI AGGCCT 1 cut(s) 1518
StyD4I CCNGG 8 cut(s) 59, 89, 766, 1095, 1324, 2785, 3057, 3172
StyI CCWWGG 3 cut(s) 457, 1402, 1798
TaaI ACNGT 8 cut(s) 487, 857, 930, 1260, 1311, 1369, 2867, 3134
TaiI ACGT 4 cut(s) 46, 836, 1336, 1957
TaqI TCGA 7 cut(s) 14, 246, 493, 1162, 1262, 1551, 2994
TatI WGTACW 2 cut(s) 2600, 2902
TscAI CASTG 5 cut(s) 873, 1015, 1372, 3328, 3334
TseFI GTSAC 5 cut(s) 53, 111, 723, 1165, 2443
TseI GCWGC 5 cut(s) 238, 241, 427, 534, 656
Tsp45I GTSAC 5 cut(s) 53, 111, 723, 1165, 2443
TspGWI ACGGA 4 cut(s) 331, 954, 3131, 3191
TspRI CASTG 5 cut(s) 873, 1015, 1372, 3328, 3334
Van91I CCANNNNNTGG 2 cut(s) 1408, 2923
Vha464I CTTAAG 1 cut(s) 1570
VpaK11BI GGWCC 3 cut(s) 134, 764, 1504
VspI ATTAAT 1 cut(s) 1895
XceI RCATGY 1 cut(s) 466
XcmI CCANNNNNNNNNTGG 3 cut(s) 613, 1796, 2822
XhoI CTCGAG 1 cut(s) 492
XmiI GTMKAC 1 cut(s) 2575
XmnI GAANNNNTTC 5 cut(s) 597, 1299, 1393, 2732, 2769
XspI CTAG 2 cut(s) 1178, 2579
Zsp2I ATGCAT 2 cut(s) 1269, 2629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.