Rw6G003430

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000105
Physical Location & Seq
Forward (+)
5437725 .. 5442218
4494 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G003430.1

Sequence Viewer

Length: 3714 bp
ATGGCGATGATTCAAGATATATTGATGGGGGACCGCAAGAACCACGAAGACAGCCTGCGGCGCGAAGGGCGGCCACGTGATCAGCATCCAATTCAGCAGGGGTTGGTGCCGCATCGTGGGTTACCACGTGATCTACTGCAGCGGTTGAAGCCGCCGCCACTTCCTGCGGTGTCGCCGAGAAGGAAGTACGATGTGTTTCTAAGTTTCAGAGGGCCAGATACGCGCAAGGGGATTACCTCCGAGCTGTATGATAGACTAGAACGGAGAGGAATTAAAACCTTTATGGATGACCCCGATCTTCAGGTAGGAGATTCCATTAGCCCGTCTCTTGTTTCAGCAATTGAGGAGTCAAGATTTGCCATTGTCATTCTTTCACCCAACTATGCTTCTTCGTCATGGTGCTTGGAAGAACTGGTCAAGATTATTCAATGCATGAAAGAGAAGGGGCTACGAGTCATGCCCGTGTTCTACAACGTGGAGCCTTCTGATGTGCGGCACCAAAGGGGAAGTTTTGAGCTCAAAGGGAAAACCCGACCCCGAGTAGAAGTAAGGGAACATGAAGAAGTTTATGGCAAGGAGGAGGACAAATTCAAGGCGTGGAGAGCTGCTTTGACAGAGGTAGCCAATCTTTCTGGGTGGGATTCAAAGAATTTTAGAACTGAGAGAGAACTTGTTGAGGCAATTGTCGGGGATATTGGGAGTAAGGCGGTGTATAAATCTTCAAGTGTTGACAAGGGCTTCGTAGGAATGGATGTCCGTGTTGATGTTTTATTACATAATTGCATACATCAAGAGTTGGATGAGGTACGAATAATAGGGATACATGGGATGCGAGGAGTTGGTAAGACAACGCTTGCTCGAGCTTTTTACGATGACTTTTGTCACGATTTCGAGCAGAGATGCTTTCTTTTCAATGTTAGAGAAAAGTTTAAAAGGAATGGCCTAGTTTCTTTACAAAAGAACCTGCTTTCCAGCATGTTCATGGACAAAACTGATTTCATAGAGGATGAATATATAGGAGCTGCTATGATTCAAAGGAGTTTACGTAACAGAAAGGTGCTTGTTGTTATTGATGATGTGGATCGGTTGGAACAATTAGAAAAATTGGCTGGAAGTCCCAACTGGTTCGGTCCAGGGAGCAGAATTATCATAACCACCACAGATTTTCACTTGTTAGAGGTACATGGTGTTGATGCTACATACAACGCTACCGGGTTAAGTTCTGATGAAGCTCTTGAACTCTTGAGTTTAAAGGCTTTCAAGAAAATGCCCCCACCAGAAAATTATTTGGACCTGTGCTACCATATTTTAGAGTATGCTCAGGGGCTGCCGTTAGCTCTTGTGGTTATAGGTTCTTTTCTATGTGGTAGAAGCACCAATGAATGGGCAAGTGCAATTGATAGGCTAAAAAATACACCAAATAGAGGAATTATGGATGTGCTTCAGATTAGTTTTGATGGACTAGAGGACATAGACAAAGAAATATTCCTACATATTGCTTGTTTTTACAAGGGGAAAGATAGGAATCGTGTGACACAAATACTAGACTATTGTGAGCTGAACCCTGTCATTGGATTAAGAGTTCTTGCTGATAAATCTCTCATTACTATTTCCAGCAACGAACTGTCAATGCATGATTTGCTACAAGAAATGGGCTGGGAAATAGTTCGTCGACAGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTATTGAAGACATCCATAATGTGTTGAAGAAAAATAAGGGAACAAAAGCAATCCAAGGCATGGTAATTGAATTGCCTAAATTAGAAGTGGCTCATTGGAATCGAAAAGCCTTCTCAAATTTGTCTCAACTTAGTCTTCTCCATATTCGTAATGTGGTCCTTCCCAAAGGCCTCACGTGTCTTTCTAATTCCTTGAGACTCCTGGAATGGCCTGGGTGTCCCTTAAGATCACTCCCACGAAAATTCGAACCAGATGAACTTATTGAACTTAACTTGTGTCACAGCAATATTAGACATCTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGCGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTAAGAGTTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTTGAGAATCTGCCAGGTAGAATTGAAATGGAATCTCTTGAAATATTAATTCTGTCTAACTGCTCAAAGATTAAAAAGATTCCCGAGTTTGATGGAAATATGGAGCGTCTGTCTGTGCTTTCTCTCGATGAGACTGCCATTGAGAAACTGCCTGTTTCAATTGGACGCCTGAGTGGCCTTGTGTCATTGAATCTAAGCAACTGCACAAATCTTGTTTGTCTTCCATGCACCATCAATAATTTGAAGTTTGTTGAAATTCTTAATCTTTCTGGGTGCATAAAACTTGGTAAACAGGTAAGAGTGGGGGAGATGGACTGTTATGAGGAAAATGATGTGAATAGTGGGTCTGCAATAGAAATGCTCATAAAGTATGTGAGAGGTTCAATCGTTCGTGGACGCGAAGTAGTTTTGGGATCTTTACGCTTGCCTTCTGGATTGGTGAAAAAAGTGAATACAAAGCCAATGAGTTTCCACTTGCCTATATCGGGTCTCTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTCCCAACGAATTTGGTTACTTTTCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGTCTTCCTTCGGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTAGCCTAGATTTAAGGGCAGATGGTTGTAGTTCACTGAAATACTTGTTTGATGCATCAAACTTGAACAGATTAAACAAATCATATTTTAATTTCATCAATTGCGTCAATATAAATGGCAATCAAGTATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTGATAAGAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCGAAGTGCTGGGTGTTCATTAAGTGTGGAGCTGCCTCCAGATTGGGATAGCAGTAGGTTTATGGGATTTGCTCTGTGTGTTGTTTTTGTACTCCATGAGCACCATCAGGTTGATGAGCTTGATATTCATCAATTCAAGAATTTTAACGCTACTCATCATCTTGTATGTTGCCTGAAGCTCAATGGAAGAGAATTAGAAGTATACGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAGTTTTGCCAGCTTAACTCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAGTACTTTGGTACAGAGTGGTGGCATAACAGTTGCAGTCAGCTTGATTTCTTATTTGAAACCAGAGGGCCAGGTCTGAAGGTGAAAGAGTGTGGAGTCCGTCTGATATATGAGCTAGATGTGCAAGAGTTGAACCAAACAACCACTCAATCAAGCAGTAGGATGTCTCCATATGAGGACATATTGGTTGATTTTGATATTCCAGTTGAAGGGGAAACCAGTGGCCCTGGTAGCAGAACTTGCAGACTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1237

Amino Acids

140.6

Weight (kDa)

6.56

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 62 - 241 5e-53 TIR domain
TIR_2 PF13676 65 - 162 3.2e-18 TIR domain
NB-ARC PF00931 266 - 422 2.4e-18 NB-ARC domain
AAA_22 PF13401 270 - 369 5e-06 AAA domain
NACHT PF05729 271 - 368 1e-05 NACHT domain
WHD_ROQ1 PF23282 491 - 560 5.5e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 749 - 838 4.2e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 754 - 828 5.7e-07 Leucine-rich repeat region
LRR_14 PF23598 899 - 999 2.7e-06 Leucine-rich repeat region
C-JID PF20160 1044 - 1192 3e-19 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 972
AccB1I GGYRCC 2 cut(s) 106, 495
AccB7I CCANNNNNTGG 2 cut(s) 1383, 1774
AccI GTMKAC 2 cut(s) 1672, 3366
AccII CGCG 3 cut(s) 63, 223, 2613
AclWI GGATC 2 cut(s) 1089, 2635
AcoI YGGCCR 1 cut(s) 71
AcsI RAATTY 8 cut(s) 587, 649, 1831, 1955, 2029, 2469, 2768, 3304
AcuI CTGAAG 5 cut(s) 284, 1427, 3095, 3359, 3551
AcvI CACGTG 3 cut(s) 77, 128, 1890
AcyI GRCGYC 1 cut(s) 2380
AfaI GTAC 6 cut(s) 188, 807, 1182, 3255, 3458, 3466
AfiI CCNNNNNNNGG 7 cut(s) 116, 1383, 1424, 1571, 1774, 2699, 3662
AflII CTTAAG 1 cut(s) 1936
AflIII ACRYGT 1 cut(s) 1889
AjnI CCWGG 8 cut(s) 1132, 1690, 1914, 1924, 2227, 3133, 3523, 3679
AjuI GAANNNNNNNTTGG 2 cut(s) 2855, 2887
AloI GAACNNNNNNTCC 4 cut(s) 953, 985, 1566, 1598
Alw21I GWGCWC 2 cut(s) 519, 3267
Alw26I GTCTC 9 cut(s) 330, 1683, 1842, 1903, 2075, 2339, 2708, 2881, 3624
AlwI GGATC 2 cut(s) 1089, 2635
AlwNI CAGNNNCTG 1 cut(s) 1327
Ama87I CYCGRG 3 cut(s) 537, 858, 2297
AoxI GGCC 8 cut(s) 71, 212, 940, 1882, 1922, 2389, 3521, 3676
ApeKI GCWGC 5 cut(s) 139, 605, 1022, 1327, 3196
ApoI RAATTY 8 cut(s) 587, 649, 1831, 1955, 2029, 2469, 2768, 3304
ArsI GACNNNNNNTTYG 2 cut(s) 722, 754
AseI ATTAAT 1 cut(s) 2261
Asp700I GAANNNNTTC 3 cut(s) 1665, 3080, 3117
AspLEI GCGC 2 cut(s) 63, 225
AspS9I GGNCC 7 cut(s) 31, 212, 1130, 1291, 1870, 3521, 3677
AsuC2I CCSGG 1 cut(s) 1213
AsuHPI GGTGA 6 cut(s) 366, 2665, 2759, 2803, 3416, 3547
AsuII TTCGAA 1 cut(s) 1959
AvaI CYCGRG 3 cut(s) 537, 858, 2297
AvaII GGWCC 4 cut(s) 31, 1130, 1291, 1870
BanI GGYRCC 2 cut(s) 106, 495
BanII GRGCYC 1 cut(s) 519
BauI CACGAG 1 cut(s) 3447
BbrPI CACGTG 3 cut(s) 77, 128, 1890
BbsI GAAGAC 6 cut(s) 54, 1728, 1841, 2426, 2819, 3083
Bbv12I GWGCWC 2 cut(s) 519, 3267
BbvI GCAGC 5 cut(s) 151, 592, 1009, 1314, 3183
BceAI ACGGC 2 cut(s) 1315, 3385
BcgI CGANNNNNNTGC 2 cut(s) 2330, 2364
BciT130I CCWGG 8 cut(s) 1134, 1692, 1916, 1926, 2229, 3135, 3525, 3681
BciVI GTATCC 1 cut(s) 813
BclI TGATCA 1 cut(s) 79
BcnI CCSGG 1 cut(s) 1213
BcoDI GTCTC 9 cut(s) 330, 1683, 1842, 1903, 2075, 2339, 2708, 2881, 3624
BfaI CTAG 6 cut(s) 257, 944, 1463, 1544, 2927, 3569
BfmI CTRYAG 1 cut(s) 137
BfrI CTTAAG 1 cut(s) 1936
BfuAI ACCTGC 1 cut(s) 972
BfuI GTATCC 1 cut(s) 813
BglI GCCNNNNNGGC 1 cut(s) 2388
BglII AGATCT 1 cut(s) 2122
BmcAI AGTACT 1 cut(s) 3458
Bme1390I CCNGG 9 cut(s) 1134, 1213, 1692, 1916, 1926, 2229, 3135, 3525, 3681
Bme18I GGWCC 4 cut(s) 31, 1130, 1291, 1870
BmeT110I CYCGRG 3 cut(s) 537, 858, 2297
BmgT120I GGNCC 7 cut(s) 31, 212, 1130, 1291, 1870, 3521, 3677
BmiI GGNNCC 4 cut(s) 32, 108, 480, 497
BmrFI CCNGG 9 cut(s) 1134, 1213, 1692, 1916, 1926, 2229, 3135, 3525, 3681
BmsI GCATC 7 cut(s) 94, 121, 819, 890, 1183, 2962, 2984
BoxI GACNNNNGTC 1 cut(s) 879
BpiI GAAGAC 6 cut(s) 54, 1728, 1841, 2426, 2819, 3083
BpmI CTGGAG 1 cut(s) 3186
Bpu10I CCTNAGC 1 cut(s) 1320
Bpu14I TTCGAA 1 cut(s) 1959
BpuEI CTTGAG 5 cut(s) 1264, 1927, 2132, 2237, 2879
BpuMI CCSGG 1 cut(s) 1213
BsaAI YACGTR 4 cut(s) 77, 128, 1046, 1890
BsaBI GATNNNNATC 4 cut(s) 84, 1080, 1524, 3291
BsaHI GRCGYC 1 cut(s) 2380
BsaI GGTCTC 2 cut(s) 2075, 2708
BsaJI CCNNGG 5 cut(s) 1133, 1768, 1925, 2164, 3679
BsaXI ACNNNNNCTCC 4 cut(s) 1030, 1060, 2309, 2339
Bsc4I CCNNNNNNNGG 7 cut(s) 116, 1383, 1424, 1571, 1774, 2699, 3662
Bse1I ACTGG 4 cut(s) 417, 1127, 3656, 3672
Bse8I GATNNNNATC 4 cut(s) 84, 1080, 1524, 3291
BseBI CCWGG 8 cut(s) 1134, 1692, 1916, 1926, 2229, 3135, 3525, 3681
BseDI CCNNGG 5 cut(s) 1133, 1768, 1925, 2164, 3679
BseJI GATNNNNATC 4 cut(s) 84, 1080, 1524, 3291
BseLI CCNNNNNNNGG 7 cut(s) 116, 1383, 1424, 1571, 1774, 2699, 3662
BseMII CTCAG 6 cut(s) 651, 1334, 2375, 2720, 3141, 3432
BseNI ACTGG 4 cut(s) 417, 1127, 3656, 3672
BseRI GAGGAG 3 cut(s) 359, 593, 849
BseXI GCAGC 5 cut(s) 151, 592, 1009, 1314, 3183
BseYI CCCAGC 2 cut(s) 1656, 3173
BsgI GTGCAG 1 cut(s) 2401
Bsh1236I CGCG 3 cut(s) 63, 223, 2613
BshFI GGCC 8 cut(s) 73, 214, 942, 1884, 1924, 2391, 3523, 3678
BshNI GGYRCC 2 cut(s) 106, 495
BsiHKAI GWGCWC 2 cut(s) 519, 3267
BsiHKCI CYCGRG 3 cut(s) 537, 858, 2297
BsiSI CCGG 1 cut(s) 1212
BslFI GGGAC 3 cut(s) 44, 1101, 1917
BslI CCNNNNNNNGG 7 cut(s) 116, 1383, 1424, 1571, 1774, 2699, 3662
BsmAI GTCTC 9 cut(s) 330, 1683, 1842, 1903, 2075, 2339, 2708, 2881, 3624
BsmBI CGTCTC 1 cut(s) 330
BsmFI GGGAC 3 cut(s) 44, 1101, 1917
BsnI GGCC 8 cut(s) 73, 214, 942, 1884, 1924, 2391, 3523, 3678
Bso31I GGTCTC 2 cut(s) 2075, 2708
BsoBI CYCGRG 3 cut(s) 537, 858, 2297
Bsp119I TTCGAA 1 cut(s) 1959
Bsp1286I GDGCHC 2 cut(s) 519, 3267
Bsp143I GATC 8 cut(s) 79, 130, 295, 1081, 1940, 2122, 2627, 3421
BspANI GGCC 8 cut(s) 73, 214, 942, 1884, 1924, 2391, 3523, 3678
BspCNI CTCAG 6 cut(s) 652, 1333, 2376, 2721, 3142, 3431
BspFNI CGCG 3 cut(s) 63, 223, 2613
BspLI GGNNCC 4 cut(s) 32, 108, 480, 497
BspMAI CTGCAG 1 cut(s) 141
BspMI ACCTGC 1 cut(s) 972
BspPI GGATC 2 cut(s) 1089, 2635
BspT104I TTCGAA 1 cut(s) 1959
BspT107I GGYRCC 2 cut(s) 106, 495
BspTI CTTAAG 1 cut(s) 1936
BspTNI GGTCTC 2 cut(s) 2075, 2708
BsrI ACTGG 4 cut(s) 417, 1127, 3656, 3672
BssECI CCNNGG 5 cut(s) 1133, 1768, 1925, 2164, 3679
BssMI GATC 8 cut(s) 79, 130, 295, 1081, 1940, 2122, 2627, 3421
BssNAI GTATAC 1 cut(s) 3367
BssNI GRCGYC 1 cut(s) 2380
BssSI CACGAG 1 cut(s) 3447
BssT1I CCWWGG 2 cut(s) 1768, 2164
Bst1107I GTATAC 1 cut(s) 3367
Bst2BI CACGAG 1 cut(s) 3447
Bst2UI CCWGG 8 cut(s) 1134, 1692, 1916, 1926, 2229, 3135, 3525, 3681
Bst4CI ACNGT 4 cut(s) 1626, 1677, 2531, 3485
Bst6I CTCTTC 2 cut(s) 3346, 3393
BstACI GRCGYC 1 cut(s) 2380
BstAFI CTTAAG 1 cut(s) 1936
BstAPI GCANNNNNTGC 2 cut(s) 1639, 3693
BstBAI YACGTR 4 cut(s) 77, 128, 1046, 1890
BstBI TTCGAA 1 cut(s) 1959
BstC8I GCNNGC 6 cut(s) 56, 855, 2098, 2639, 3381, 3410
BstEII GGTNACC 2 cut(s) 120, 2791
BstFNI CGCG 3 cut(s) 63, 223, 2613
BstHHI GCGC 2 cut(s) 63, 225
BstKTI GATC 8 cut(s) 82, 133, 298, 1084, 1943, 2125, 2630, 3424
BstMAI GTCTC 9 cut(s) 330, 1683, 1842, 1903, 2075, 2339, 2708, 2881, 3624
BstMBI GATC 8 cut(s) 79, 130, 295, 1081, 1940, 2122, 2627, 3421
BstNI CCWGG 8 cut(s) 1134, 1692, 1916, 1926, 2229, 3135, 3525, 3681
BstNSI RCATGY 1 cut(s) 979
BstPAI GACNNNNGTC 1 cut(s) 879
BstPI GGTNACC 2 cut(s) 120, 2791
BstSCI CCNGG 9 cut(s) 1132, 1211, 1690, 1914, 1924, 2227, 3133, 3523, 3679
BstSFI CTRYAG 1 cut(s) 137
BstSNI TACGTA 1 cut(s) 1046
BstUI CGCG 3 cut(s) 63, 223, 2613
BstV1I GCAGC 5 cut(s) 151, 592, 1009, 1314, 3183
BstV2I GAAGAC 6 cut(s) 54, 1728, 1841, 2426, 2819, 3083
BstX2I RGATCY 2 cut(s) 2122, 2627
BstYI RGATCY 2 cut(s) 2122, 2627
BstZ17I GTATAC 1 cut(s) 3367
BsuI GTATCC 1 cut(s) 813
BsuRI GGCC 8 cut(s) 73, 214, 942, 1884, 1924, 2391, 3523, 3678
BtgZI GCGATG 1 cut(s) 20
BtsIMutI CAGTG 2 cut(s) 2954, 3679
BveI ACCTGC 1 cut(s) 972
Cac8I GCNNGC 6 cut(s) 56, 855, 2098, 2639, 3381, 3410
CaiI CAGNNNCTG 1 cut(s) 1327
CfoI GCGC 2 cut(s) 63, 225
Cfr13I GGNCC 7 cut(s) 31, 212, 1130, 1291, 1870, 3521, 3677
CseI GACGC 4 cut(s) 2309, 2388, 2619, 3013
Csp6I GTAC 6 cut(s) 187, 806, 1181, 3254, 3457, 3465
CviQI GTAC 6 cut(s) 187, 806, 1181, 3254, 3457, 3465
DpnI GATC 8 cut(s) 81, 132, 297, 1083, 1942, 2124, 2629, 3423
DpnII GATC 8 cut(s) 79, 130, 295, 1081, 1940, 2122, 2627, 3421
DraI TTTAAA 2 cut(s) 931, 1251
EaeI YGGCCR 1 cut(s) 71
Eam1104I CTCTTC 2 cut(s) 3346, 3393
EarI CTCTTC 2 cut(s) 3346, 3393
Ecl136II GAGCTC 1 cut(s) 517
Eco105I TACGTA 1 cut(s) 1046
Eco130I CCWWGG 2 cut(s) 1768, 2164
Eco147I AGGCCT 1 cut(s) 1884
Eco24I GRGCYC 1 cut(s) 519
Eco31I GGTCTC 2 cut(s) 2075, 2708
Eco47I GGWCC 4 cut(s) 31, 1130, 1291, 1870
Eco53kI GAGCTC 1 cut(s) 517
Eco57I CTGAAG 5 cut(s) 284, 1427, 3095, 3359, 3551
Eco72I CACGTG 3 cut(s) 77, 128, 1890
Eco88I CYCGRG 3 cut(s) 537, 858, 2297
Eco91I GGTNACC 2 cut(s) 120, 2791
EcoICRI GAGCTC 1 cut(s) 517
EcoO65I GGTNACC 2 cut(s) 120, 2791
EcoRII CCWGG 8 cut(s) 1132, 1690, 1914, 1924, 2227, 3133, 3523, 3679
EcoT14I CCWWGG 2 cut(s) 1768, 2164
EcoT22I ATGCAT 3 cut(s) 434, 1635, 2977
EcoT38I GRGCYC 1 cut(s) 519
ErhI CCWWGG 2 cut(s) 1768, 2164
Esp3I CGTCTC 1 cut(s) 330
FaqI GGGAC 3 cut(s) 44, 1101, 1917
FauNDI CATATG 1 cut(s) 3625
FbaI TGATCA 1 cut(s) 79
FblI GTMKAC 2 cut(s) 1672, 3366
FriOI GRGCYC 1 cut(s) 519
FspBI CTAG 6 cut(s) 257, 944, 1463, 1544, 2927, 3569
GlaI GCGC 2 cut(s) 62, 224
GsaI CCCAGC 2 cut(s) 1660, 3177
GsuI CTGGAG 1 cut(s) 3186
HaeIII GGCC 8 cut(s) 73, 214, 942, 1884, 1924, 2391, 3523, 3678
HapII CCGG 1 cut(s) 1212
HgaI GACGC 4 cut(s) 2309, 2388, 2619, 3013
HhaI GCGC 2 cut(s) 63, 225
Hin1I GRCGYC 1 cut(s) 2380
Hin6I GCGC 2 cut(s) 61, 223
HinP1I GCGC 2 cut(s) 61, 223
HincII GTYRAC 2 cut(s) 730, 1673
HindII GTYRAC 2 cut(s) 730, 1673
HindIII AAGCTT 2 cut(s) 2180, 2855
HpaII CCGG 1 cut(s) 1212
HphI GGTGA 6 cut(s) 366, 2665, 2759, 2803, 3416, 3547
Hpy166II GTNNAC 7 cut(s) 730, 1043, 1673, 2504, 2609, 2954, 3367
Hpy8I GTNNAC 7 cut(s) 730, 1043, 1673, 2504, 2609, 2954, 3367
Hpy99I CGWCG 1 cut(s) 1674
HpyCH4III ACNGT 4 cut(s) 1626, 1677, 2531, 3485
HpyCH4IV ACGT 6 cut(s) 76, 127, 474, 1045, 1699, 1889
HpySE526I ACGT 6 cut(s) 76, 127, 474, 1045, 1699, 1889
Hsp92I GRCGYC 1 cut(s) 2380
HspAI GCGC 2 cut(s) 61, 223
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 8 cut(s) 79, 130, 295, 1081, 1940, 2122, 2627, 3421
LmnI GCTCC 6 cut(s) 478, 1019, 1137, 2317, 2753, 3193
Lsp1109I GCAGC 5 cut(s) 151, 592, 1009, 1314, 3183
LweI GCATC 7 cut(s) 94, 121, 819, 890, 1183, 2962, 2984
MaeI CTAG 6 cut(s) 257, 944, 1463, 1544, 2927, 3569
MaeII ACGT 6 cut(s) 76, 127, 474, 1045, 1699, 1889
MaeIII GTNAC 7 cut(s) 120, 881, 1046, 1531, 1991, 2774, 2791
MalI GATC 8 cut(s) 81, 132, 297, 1083, 1942, 2124, 2629, 3423
MboI GATC 8 cut(s) 79, 130, 295, 1081, 1940, 2122, 2627, 3421
MfeI CAATTG 5 cut(s) 339, 681, 1395, 2373, 3019
MflI RGATCY 2 cut(s) 2122, 2627
MhlI GDGCHC 2 cut(s) 519, 3267
MlyI GAGTC 4 cut(s) 356, 462, 1905, 3558
MmeI TCCRAC 2 cut(s) 777, 1068
Mph1103I ATGCAT 3 cut(s) 434, 1635, 2977
MroXI GAANNNNTTC 3 cut(s) 1665, 3080, 3117
MslI CAYNNNNRTG 3 cut(s) 461, 980, 3168
MspA1I CMGCKG 1 cut(s) 142
MspCI CTTAAG 1 cut(s) 1936
MspI CCGG 1 cut(s) 1212
MspR9I CCNGG 9 cut(s) 1134, 1213, 1692, 1916, 1926, 2229, 3135, 3525, 3681
MunI CAATTG 5 cut(s) 339, 681, 1395, 2373, 3019
MvaI CCWGG 8 cut(s) 1134, 1692, 1916, 1926, 2229, 3135, 3525, 3681
MvnI CGCG 3 cut(s) 63, 223, 2613
NciI CCSGG 1 cut(s) 1213
NdeI CATATG 1 cut(s) 3625
NdeII GATC 8 cut(s) 79, 130, 295, 1081, 1940, 2122, 2627, 3421
NlaIV GGNNCC 4 cut(s) 32, 108, 480, 497
NmeAIII GCCGAG 1 cut(s) 201
NmuCI GTSAC 4 cut(s) 881, 1531, 1991, 2791
NsiI ATGCAT 3 cut(s) 434, 1635, 2977
NspI RCATGY 1 cut(s) 979
NspV TTCGAA 1 cut(s) 1959
PaeR7I CTCGAG 1 cut(s) 858
PceI AGGCCT 1 cut(s) 1884
PdmI GAANNNNTTC 3 cut(s) 1665, 3080, 3117
PflMI CCANNNNNTGG 2 cut(s) 1383, 1774
PfoI TCCNGGA 2 cut(s) 1914, 3133
PleI GAGTC 4 cut(s) 355, 461, 1905, 3557
PmaCI CACGTG 3 cut(s) 77, 128, 1890
PmlI CACGTG 3 cut(s) 77, 128, 1890
PpsI GAGTC 4 cut(s) 355, 461, 1905, 3557
Ppu21I YACGTR 4 cut(s) 77, 128, 1046, 1890
PshAI GACNNNNGTC 1 cut(s) 879
PshBI ATTAAT 1 cut(s) 2261
Psp124BI GAGCTC 1 cut(s) 519
Psp6I CCWGG 8 cut(s) 1132, 1690, 1914, 1924, 2227, 3133, 3523, 3679
PspCI CACGTG 3 cut(s) 77, 128, 1890
PspEI GGTNACC 2 cut(s) 120, 2791
PspFI CCCAGC 2 cut(s) 1656, 3173
PspGI CCWGG 8 cut(s) 1132, 1690, 1914, 1924, 2227, 3133, 3523, 3679
PspN4I GGNNCC 4 cut(s) 32, 108, 480, 497
PspPI GGNCC 7 cut(s) 31, 212, 1130, 1291, 1870, 3521, 3677
PspXI VCTCGAGB 1 cut(s) 858
PstI CTGCAG 1 cut(s) 141
PstNI CAGNNNCTG 1 cut(s) 1327
PsuI RGATCY 2 cut(s) 2122, 2627
RsaI GTAC 6 cut(s) 188, 807, 1182, 3255, 3458, 3466
RsaNI GTAC 6 cut(s) 187, 806, 1181, 3254, 3457, 3465
RseI CAYNNNNRTG 3 cut(s) 461, 980, 3168
SacI GAGCTC 1 cut(s) 519
SalI GTCGAC 1 cut(s) 1671
Sau3AI GATC 8 cut(s) 79, 130, 295, 1081, 1940, 2122, 2627, 3421
Sau96I GGNCC 7 cut(s) 31, 212, 1130, 1291, 1870, 3521, 3677
ScaI AGTACT 1 cut(s) 3458
SchI GAGTC 4 cut(s) 356, 462, 1905, 3558
ScrFI CCNGG 9 cut(s) 1134, 1213, 1692, 1916, 1926, 2229, 3135, 3525, 3681
SduI GDGCHC 2 cut(s) 519, 3267
SfaNI GCATC 7 cut(s) 94, 121, 819, 890, 1183, 2962, 2984
SfcI CTRYAG 1 cut(s) 137
Sfr274I CTCGAG 1 cut(s) 858
SfuI TTCGAA 1 cut(s) 1959
SinI GGWCC 4 cut(s) 31, 1130, 1291, 1870
SlaI CTCGAG 1 cut(s) 858
SmiMI CAYNNNNRTG 3 cut(s) 461, 980, 3168
SmlI CTYRAG 7 cut(s) 858, 1243, 1906, 1936, 2111, 2216, 2858
SmoI CTYRAG 7 cut(s) 858, 1243, 1906, 1936, 2111, 2216, 2858
SnaBI TACGTA 1 cut(s) 1046
SseBI AGGCCT 1 cut(s) 1884
SspI AATATT 4 cut(s) 1485, 2002, 2259, 3145
SspMI CTAG 6 cut(s) 257, 944, 1463, 1544, 2927, 3569
SstI GAGCTC 1 cut(s) 519
StuI AGGCCT 1 cut(s) 1884
StyD4I CCNGG 9 cut(s) 1132, 1211, 1690, 1914, 1924, 2227, 3133, 3523, 3679
StyI CCWWGG 2 cut(s) 1768, 2164
TaaI ACNGT 4 cut(s) 1626, 1677, 2531, 3485
TaiI ACGT 6 cut(s) 79, 130, 477, 1048, 1702, 1892
TaqI TCGA 7 cut(s) 859, 891, 1672, 1816, 1959, 2340, 3166
TaqII GACCGA 1 cut(s) 1118
TatI WGTACW 2 cut(s) 3253, 3456
TauI GCSGC 6 cut(s) 61, 73, 112, 154, 157, 496
TscAI CASTG 2 cut(s) 2961, 3679
TseFI GTSAC 4 cut(s) 881, 1531, 1991, 2791
TseI GCWGC 5 cut(s) 139, 605, 1022, 1327, 3196
Tsp45I GTSAC 4 cut(s) 881, 1531, 1991, 2791
TspGWI ACGGA 3 cut(s) 277, 746, 3542
TspRI CASTG 2 cut(s) 2961, 3679
Van91I CCANNNNNTGG 2 cut(s) 1383, 1774
Vha464I CTTAAG 1 cut(s) 1936
VpaK11BI GGWCC 4 cut(s) 31, 1130, 1291, 1870
VspI ATTAAT 1 cut(s) 2261
XapI RAATTY 8 cut(s) 587, 649, 1831, 1955, 2029, 2469, 2768, 3304
XceI RCATGY 1 cut(s) 979
XcmI CCANNNNNNNNNTGG 3 cut(s) 979, 2162, 3170
XhoI CTCGAG 1 cut(s) 858
XmiI GTMKAC 2 cut(s) 1672, 3366
XmnI GAANNNNTTC 3 cut(s) 1665, 3080, 3117
XspI CTAG 6 cut(s) 257, 944, 1463, 1544, 2927, 3569
ZrmI AGTACT 1 cut(s) 3458
Zsp2I ATGCAT 3 cut(s) 434, 1635, 2977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.