Rw1G003430

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Forward (+)
6408403 .. 6412722
4320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G003430.1

Sequence Viewer

Length: 3666 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTCTTTCTAAGTTTCAGGGGGGAAGATACGCGCAGGGGTTTTACCGATCATCTATATTCTGAACTAATTCGAAAAGGAATTCTCACCTTTAAGGATGACGCAGATCTTGAGAAAGGAAAATCCATTAGACGTAAACTTTTAGCTGCAATTGAGGACTCGAGATATGCGATTGTCATTCTCTCACGGGACTATGCTTATTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGACATGGGCCAACAAGTCCTCCCTGTCTTCTACGGCGTGGATCCTTCTGATGTGCGGCACCAAAGGCGAAGTTTTGAGCTCAAAAGGAAAACCCAAGTAGATGTGGAAATAAGTGAACATGAAGAAGTTTATGAGCAGAATAATGAGGACAGACTAAATGCGTGGAGAGCTGCTTTGACACAGGTGGCCAATCTTTCTGGCTGGGATTCGGGGAATTATGGATATGATTCAAACATAGTTAACGAAATTGTCAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGAGAAGGGCTTCATAGGAATGAATTCCCGCATTAGTGATTTATTAAGCAATTACATACATCCGCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATCAAATCTGTCAGGATTTTGACGAAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCCTAGTTTTTCTGCAAGAAAAACTTCTTTCCAAAATCCTGATGGCAAAAGTTAAAAGTATAGAGGATGAATACACGGGGGCTGCTATGATAAAAAGGCGGTTGTGTAGAAAAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAATCGTAACTGGTTTGGTCCAGGGACTAGAATTATCATAACCACCACAGATCTTCAGTTGTTAAAGGCACATTCTGTTGATGCTATATACAAGGCTAACGGCTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATGTCCCCCACCAGAAGATTATTTGGATCTGTGCTACGATATTATAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCCTTTCTATCTGGTAGAGGGGCTGATAAATGGGCAAGTGCAATAGATAGGCTAAGGAGCACACCAGATAAAGGCATTATTGACGTGCTTCGGATTAGTTTTGATGCCCTGCCTAATAAAGATAAAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAGAACCTGTCATTGGTTTAGATGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAACGAACTGTCAATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTGAAGAGCCAGGCAAGCGTAGTAGATTATGGTCTCATGACGACATCAACAATGTGCTGAAGAAAAATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAATTACGAGCGGCTCATTGGAATCCAGAAGCCTTTTCAAATTTGTCTCAACTTGGTCTTCTCCATATTCGTAATGTGGACCTTCCCAAAGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTTGAATGGACTGGGTATCCCTTACGATCTCTCCCAAAATTTTTTGAAGCAGATGAACTTATTGAACTTAACTTGTGCCACAGCAACATTAAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGTCATTCTCAAAAACTTGTGGAGACCCCAGATCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCATTTGGTAAGAATCCATCAGTCCCTGGGGTTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTCGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTGGCCTTGTGTTATTGAATCTAAGCAACTGCAGAAAACTTGTTTGTCTTCCAAGCACCATCAATAAATTGATGTCCGTTGAAAATCTTAATCTTTCTGGATGCTTGGAACTCGGCAAACATCAGGTAAATATGGGGGAGATGGACTGTTTTCAGGAAACTGATGTGAATAGTGGGTCTGCCATAGAAATGTCATCTACCCATGATCGCATAAAACATGTGAGAGCTTCAATCTTTCATCGATGCAAAGTAGTTTGGGGATCTTTAAATAAGTTTTTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGAGCCAACGAGTTTCCGCTTGCCTATTTTTGCTCTGTGTAATTTAACGTATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAATGAATTCGGTTGCTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGCGTTCCTTCAGGCATTCAATTGCTTTCCAAGCTTGAGAAGTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTTGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAAAAGGTCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATGAAGGATGCAATAACATAGCATTTGAAATGCTTAGGACATCCATATATCAGGGAATCTCTAATAAAAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCAGAGTGTTGGGTGTTCATTAAGTGTATCTCTACCTGTACATTGGAATAACAGTCGGTTTTTGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATGAGCACCATCCGGTGGATGAGCTTTATATGGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCAATGGAGGAGAATTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTGAGTCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAACTGTAGCAGTCAGCTTGAGTTCTTATTTGAAACCAGAGGACCGGGTCTGAAAGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAAGATGTGCAAGAGTTGAACCAAACAACCACTTATGAGGATATATCTATTGGTTTTGACATTCCAGTTGCAGGGGAAGCCAGTGGCACTGGTAGCAGAACTTGCACGCTTGAAGAGTTGAACCAAACAACCACTTATGAGGATATATTGATTGGTTTTGACATTCCAGTTGCAGGGGAAACCAGTGGCACTGGTAGCAGAACTTGCACGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

1221

Amino Acids

138.31

Weight (kDa)

6.07

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 192 6.7e-49 TIR domain
TIR_2 PF13676 14 - 123 7.6e-15 TIR domain
NB-ARC PF00931 218 - 372 1.4e-18 NB-ARC domain
NACHT PF05729 222 - 318 1.8e-06 NACHT domain
WHD_ROQ1 PF23282 440 - 510 4.7e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 699 - 783 6.2e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 710 - 779 5.5e-08 Leucine-rich repeat region
C-JID PF20160 1002 - 1151 1.8e-20 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1937
Acc36I ACCTGC 2 cut(s) 758, 1937
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 2 cut(s) 1624, 3148
AccBSI CCGCTC 1 cut(s) 1652
AccI GTMKAC 2 cut(s) 563, 2797
AccII CGCG 2 cut(s) 70, 2700
AciI CCGC 7 cut(s) 340, 607, 641, 682, 889, 1652, 2560
AclI AACGTT 1 cut(s) 2170
AclWI GGATC 8 cut(s) 320, 333, 664, 677, 939, 1149, 1383, 2503
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 9 cut(s) 117, 601, 1681, 1802, 1879, 2642, 2854, 3170, 3275
AcuI CTGAAG 5 cut(s) 1001, 1554, 1610, 2691, 3233
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 2824, 3078, 3129, 3340
AfiI CCNNNNNNNGG 7 cut(s) 647, 1274, 1421, 1624, 3148, 3512, 3614
AflIII ACRYGT 1 cut(s) 2452
AjiI CACGTC 1 cut(s) 1288
AjnI CCWGG 5 cut(s) 982, 1540, 2013, 3007, 3282
AjuI GAANNNNNNNTTGG 4 cut(s) 1404, 1436, 2729, 2761
Alw21I GWGCWC 3 cut(s) 366, 1265, 3141
AlwI GGATC 8 cut(s) 320, 333, 664, 677, 939, 1149, 1383, 2503
AlwNI CAGNNNCTG 1 cut(s) 2014
Ama87I CYCGRG 4 cut(s) 196, 708, 2066, 2147
AoxI GGCC 5 cut(s) 292, 471, 790, 1732, 2239
ApeKI GCWGC 4 cut(s) 182, 455, 643, 872
ApoI RAATTY 9 cut(s) 117, 601, 1681, 1802, 1879, 2642, 2854, 3170, 3275
AseI ATTAAT 1 cut(s) 2111
Asp700I GAANNNNTTC 8 cut(s) 105, 587, 601, 813, 1083, 1515, 1609, 2991
AspLEI GCGC 1 cut(s) 72
AspS9I GGNCC 4 cut(s) 292, 980, 1720, 3395
AsuC2I CCSGG 1 cut(s) 3399
AsuHPI GGTGA 4 cut(s) 115, 2633, 2677, 3290
AsuII TTCGAA 1 cut(s) 109
AvaI CYCGRG 4 cut(s) 196, 708, 2066, 2147
AvaII GGWCC 3 cut(s) 980, 1720, 3395
BalI TGGCCA 1 cut(s) 473
BamHI GGATCC 2 cut(s) 325, 669
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 2 cut(s) 1190, 3321
BbsI GAAGAC 3 cut(s) 304, 1691, 2276
Bbv12I GWGCWC 3 cut(s) 366, 1265, 3141
BbvI GCAGC 4 cut(s) 169, 442, 655, 859
BccI CCATC 8 cut(s) 826, 2013, 2303, 2371, 2794, 2810, 3045, 3150
BceAI ACGGC 3 cut(s) 334, 1078, 2246
BcgI CGANNNNNNTGC 4 cut(s) 2047, 2081, 2180, 2214
BciT130I CCWGG 5 cut(s) 984, 1542, 2015, 3009, 3284
BciVI GTATCC 1 cut(s) 1791
BclI TGATCA 1 cut(s) 721
BcnI CCSGG 1 cut(s) 3399
BfaI CTAG 4 cut(s) 794, 990, 1394, 1409
BfmI CTRYAG 2 cut(s) 2266, 3358
BfuAI ACCTGC 2 cut(s) 758, 1937
BfuI GTATCC 1 cut(s) 1791
BglII AGATCT 5 cut(s) 142, 1012, 1445, 1939, 1972
BisI GCNGC 6 cut(s) 183, 341, 456, 644, 873, 1653
BlsI GCNGC 6 cut(s) 184, 342, 457, 645, 874, 1654
Bme1390I CCNGG 6 cut(s) 984, 1542, 2015, 3009, 3284, 3399
Bme18I GGWCC 3 cut(s) 980, 1720, 3395
BmeT110I CYCGRG 4 cut(s) 196, 708, 2066, 2147
BmgBI CACGTC 1 cut(s) 1288
BmgT120I GGNCC 4 cut(s) 292, 980, 1720, 3395
BmiI GGNNCC 4 cut(s) 327, 344, 671, 1204
BmrFI CCNGG 6 cut(s) 984, 1542, 2015, 3009, 3284, 3399
BmrI ACTGGG 1 cut(s) 1785
BmsI GCATC 7 cut(s) 1033, 1297, 2327, 2468, 2836, 2858, 2912
BmuI ACTGGG 1 cut(s) 1785
BpiI GAAGAC 3 cut(s) 304, 1691, 2276
Bpu10I CCTNAGC 3 cut(s) 1170, 1256, 2948
Bpu14I TTCGAA 1 cut(s) 109
BpuEI CTTGAG 5 cut(s) 167, 1777, 1982, 2753, 3392
BpuMI CCSGG 1 cut(s) 3399
Bsa29I ATCGAT 1 cut(s) 2476
BsaBI GATNNNNATC 2 cut(s) 930, 1374
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1569, 1925
BsaJI CCNNGG 5 cut(s) 673, 983, 1618, 2013, 2014
BsaWI WCCGGW 1 cut(s) 3145
BsaXI ACNNNNNCTCC 4 cut(s) 288, 318, 1779, 1809
Bsc4I CCNNNNNNNGG 7 cut(s) 647, 1274, 1421, 1624, 3148, 3512, 3614
Bse1I ACTGG 9 cut(s) 977, 1780, 2242, 3506, 3522, 3535, 3608, 3624, 3637
Bse3DI GCAATG 1 cut(s) 2920
Bse8I GATNNNNATC 2 cut(s) 930, 1374
BseBI CCWGG 5 cut(s) 984, 1542, 2015, 3009, 3284
BseCI ATCGAT 1 cut(s) 2476
BseDI CCNNGG 5 cut(s) 673, 983, 1618, 2013, 2014
BseJI GATNNNNATC 2 cut(s) 930, 1374
BseLI CCNNNNNNNGG 7 cut(s) 647, 1274, 1421, 1624, 3148, 3512, 3614
BseMI GCAATG 1 cut(s) 2920
BseMII CTCAG 3 cut(s) 1184, 2594, 3015
BseNI ACTGG 9 cut(s) 977, 1780, 2242, 3506, 3522, 3535, 3608, 3624, 3637
BseRI GAGGAG 1 cut(s) 3240
BseXI GCAGC 4 cut(s) 169, 442, 655, 859
BseYI CCCAGC 2 cut(s) 486, 1506
Bsh1236I CGCG 2 cut(s) 70, 2700
BshFI GGCC 5 cut(s) 294, 473, 792, 1734, 2241
BshNI GGYRCC 1 cut(s) 342
BshVI ATCGAT 1 cut(s) 2476
BsiHKAI GWGCWC 3 cut(s) 366, 1265, 3141
BsiHKCI CYCGRG 4 cut(s) 196, 708, 2066, 2147
BsiSI CCGG 2 cut(s) 3146, 3398
BslFI GGGAC 4 cut(s) 239, 1000, 1104, 1996
BslI CCNNNNNNNGG 7 cut(s) 647, 1274, 1421, 1624, 3148, 3512, 3614
BsmFI GGGAC 4 cut(s) 239, 1000, 1104, 1996
BsmI GAATGC 1 cut(s) 2712
BsnI GGCC 5 cut(s) 294, 473, 792, 1734, 2241
Bso31I GGTCTC 2 cut(s) 1569, 1925
BsoBI CYCGRG 4 cut(s) 196, 708, 2066, 2147
Bsp119I TTCGAA 1 cut(s) 109
Bsp1286I GDGCHC 3 cut(s) 366, 1265, 3141
Bsp1407I TGTACA 1 cut(s) 3076
Bsp19I CCATGG 1 cut(s) 673
BspACI CCGC 7 cut(s) 340, 607, 641, 682, 889, 1652, 2560
BspANI GGCC 5 cut(s) 294, 473, 792, 1734, 2241
BspCNI CTCAG 3 cut(s) 1183, 2595, 3016
BspDI ATCGAT 1 cut(s) 2476
BspFNI CGCG 2 cut(s) 70, 2700
BspHI TCATGA 1 cut(s) 1567
BspLI GGNNCC 4 cut(s) 327, 344, 671, 1204
BspMAI CTGCAG 1 cut(s) 2270
BspMI ACCTGC 2 cut(s) 758, 1937
BspPI GGATC 8 cut(s) 320, 333, 664, 677, 939, 1149, 1383, 2503
BspQI GCTCTTC 1 cut(s) 1530
BspT104I TTCGAA 1 cut(s) 109
BspT107I GGYRCC 1 cut(s) 342
BspTNI GGTCTC 2 cut(s) 1569, 1925
BsrBI CCGCTC 1 cut(s) 1652
BsrDI GCAATG 1 cut(s) 2920
BsrGI TGTACA 1 cut(s) 3076
BsrI ACTGG 9 cut(s) 977, 1780, 2242, 3506, 3522, 3535, 3608, 3624, 3637
BssECI CCNNGG 5 cut(s) 673, 983, 1618, 2013, 2014
BssNAI GTATAC 1 cut(s) 564
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 1190, 3321
BssT1I CCWWGG 2 cut(s) 673, 1618
Bst1107I GTATAC 1 cut(s) 564
Bst2BI CACGAG 2 cut(s) 1190, 3321
Bst2UI CCWGG 5 cut(s) 984, 1542, 2015, 3009, 3284
Bst4CI ACNGT 6 cut(s) 1073, 1476, 1527, 2384, 3092, 3359
Bst6I CTCTTC 3 cut(s) 19, 1530, 3549
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 4 cut(s) 1489, 3448, 3543, 3645
BstAUI TGTACA 1 cut(s) 3076
BstBI TTCGAA 1 cut(s) 109
BstC8I GCNNGC 8 cut(s) 680, 1351, 1406, 1547, 2564, 3255, 3548, 3650
BstDEI CTNAG 8 cut(s) 47, 1170, 1256, 2258, 2603, 2678, 2948, 3024
BstDSI CCRYGG 1 cut(s) 673
BstEII GGTNACC 1 cut(s) 2665
BstFNI CGCG 2 cut(s) 70, 2700
BstHHI GCGC 1 cut(s) 72
BstNI CCWGG 5 cut(s) 984, 1542, 2015, 3009, 3284
BstNSI RCATGY 2 cut(s) 682, 2456
BstPI GGTNACC 1 cut(s) 2665
BstSCI CCNGG 6 cut(s) 982, 1540, 2013, 3007, 3282, 3397
BstSFI CTRYAG 2 cut(s) 2266, 3358
BstUI CGCG 2 cut(s) 70, 2700
BstV1I GCAGC 4 cut(s) 169, 442, 655, 859
BstV2I GAAGAC 3 cut(s) 304, 1691, 2276
BstX2I RGATCY 9 cut(s) 142, 325, 669, 1012, 1141, 1445, 1939, 1972, 2495
BstYI RGATCY 9 cut(s) 142, 325, 669, 1012, 1141, 1445, 1939, 1972, 2495
BstZ17I GTATAC 1 cut(s) 564
Bsu15I ATCGAT 1 cut(s) 2476
BsuI GTATCC 1 cut(s) 1791
BsuRI GGCC 5 cut(s) 294, 473, 792, 1734, 2241
BsuTUI ATCGAT 1 cut(s) 2476
BtgI CCRYGG 1 cut(s) 673
BtrI CACGTC 1 cut(s) 1288
BtsIMutI CAGTG 5 cut(s) 2828, 3528, 3529, 3630, 3631
BveI ACCTGC 2 cut(s) 758, 1937
Cac8I GCNNGC 8 cut(s) 680, 1351, 1406, 1547, 2564, 3255, 3548, 3650
CaiI CAGNNNCTG 1 cut(s) 2014
CciI TCATGA 1 cut(s) 1567
CfoI GCGC 1 cut(s) 72
Cfr13I GGNCC 4 cut(s) 292, 980, 1720, 3395
ClaI ATCGAT 1 cut(s) 2476
CseI GACGC 1 cut(s) 146
Csp6I GTAC 4 cut(s) 2823, 3077, 3128, 3339
CviQI GTAC 4 cut(s) 2823, 3077, 3128, 3339
DdeI CTNAG 8 cut(s) 47, 1170, 1256, 2258, 2603, 2678, 2948, 3024
DraI TTTAAA 1 cut(s) 2502
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 3 cut(s) 19, 1530, 3549
EarI CTCTTC 3 cut(s) 19, 1530, 3549
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 2 cut(s) 673, 1618
Eco147I AGGCCT 1 cut(s) 1734
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 2 cut(s) 1569, 1925
Eco47I GGWCC 3 cut(s) 980, 1720, 3395
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 5 cut(s) 1001, 1554, 1610, 2691, 3233
Eco88I CYCGRG 4 cut(s) 196, 708, 2066, 2147
Eco91I GGTNACC 1 cut(s) 2665
EcoICRI GAGCTC 1 cut(s) 364
EcoO65I GGTNACC 1 cut(s) 2665
EcoRI GAATTC 4 cut(s) 117, 601, 2642, 3170
EcoRII CCWGG 5 cut(s) 982, 1540, 2013, 3007, 3282
EcoT14I CCWWGG 2 cut(s) 673, 1618
EcoT22I ATGCAT 3 cut(s) 281, 1485, 2851
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 2 cut(s) 673, 1618
FalI AAGNNNNNCTT 6 cut(s) 798, 830, 2729, 2761, 2974, 3006
FaqI GGGAC 4 cut(s) 239, 1000, 1104, 1996
FauI CCCGC 2 cut(s) 614, 675
FbaI TGATCA 1 cut(s) 721
FblI GTMKAC 2 cut(s) 563, 2797
Fnu4HI GCNGC 6 cut(s) 183, 341, 456, 644, 873, 1653
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 6 cut(s) 183, 341, 456, 644, 873, 1653
FspBI CTAG 4 cut(s) 794, 990, 1394, 1409
GlaI GCGC 1 cut(s) 71
GluI GCNGC 6 cut(s) 183, 341, 456, 644, 873, 1653
GsaI CCCAGC 2 cut(s) 490, 1510
HaeIII GGCC 5 cut(s) 294, 473, 792, 1734, 2241
HapII CCGG 2 cut(s) 3146, 3398
HgaI GACGC 1 cut(s) 146
HhaI GCGC 1 cut(s) 72
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 70
HinP1I GCGC 1 cut(s) 70
HincII GTYRAC 4 cut(s) 9, 526, 939, 1638
HindII GTYRAC 4 cut(s) 9, 526, 939, 1638
HindIII AAGCTT 2 cut(s) 2030, 2729
HpaI GTTAAC 1 cut(s) 526
HpaII CCGG 2 cut(s) 3146, 3398
HphI GGTGA 4 cut(s) 115, 2633, 2677, 3290
Hpy99I CGWCG 1 cut(s) 24
HpyCH4III ACNGT 6 cut(s) 1073, 1476, 1527, 2384, 3092, 3359
HpyCH4IV ACGT 4 cut(s) 169, 1287, 2170, 2591
HpyF3I CTNAG 8 cut(s) 47, 1170, 1256, 2258, 2603, 2678, 2948, 3024
HpySE526I ACGT 4 cut(s) 169, 1287, 2170, 2591
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 70
Ksp22I TGATCA 1 cut(s) 721
KspAI GTTAAC 1 cut(s) 526
LguI GCTCTTC 1 cut(s) 1530
LmnI GCTCC 2 cut(s) 1260, 2627
Lsp1109I GCAGC 4 cut(s) 169, 442, 655, 859
LweI GCATC 7 cut(s) 1033, 1297, 2327, 2468, 2836, 2858, 2912
MaeI CTAG 4 cut(s) 794, 990, 1394, 1409
MaeII ACGT 4 cut(s) 169, 1287, 2170, 2591
MaeIII GTNAC 3 cut(s) 968, 1381, 2665
MbiI CCGCTC 1 cut(s) 1652
MfeI CAATTG 4 cut(s) 186, 2223, 2717, 2893
MflI RGATCY 9 cut(s) 142, 325, 669, 1012, 1141, 1445, 1939, 1972, 2495
MhlI GDGCHC 3 cut(s) 366, 1265, 3141
MlsI TGGCCA 1 cut(s) 473
MluNI TGGCCA 1 cut(s) 473
MlyI GAGTC 5 cut(s) 188, 1755, 2080, 3299, 3432
MmeI TCCRAC 2 cut(s) 1488, 2137
Mox20I TGGCCA 1 cut(s) 473
Mph1103I ATGCAT 3 cut(s) 281, 1485, 2851
MroXI GAANNNNTTC 8 cut(s) 105, 587, 601, 813, 1083, 1515, 1609, 2991
MscI TGGCCA 1 cut(s) 473
MslI CAYNNNNRTG 4 cut(s) 596, 1628, 2423, 3042
Msp20I TGGCCA 1 cut(s) 473
MspI CCGG 2 cut(s) 3146, 3398
MspR9I CCNGG 6 cut(s) 984, 1542, 2015, 3009, 3284, 3399
MunI CAATTG 4 cut(s) 186, 2223, 2717, 2893
Mva1269I GAATGC 1 cut(s) 2712
MvaI CCWGG 5 cut(s) 984, 1542, 2015, 3009, 3284
MvnI CGCG 2 cut(s) 70, 2700
NciI CCSGG 1 cut(s) 3399
NcoI CCATGG 1 cut(s) 673
NlaIV GGNNCC 4 cut(s) 327, 344, 671, 1204
NmeAIII GCCGAG 1 cut(s) 2328
NmuCI GTSAC 2 cut(s) 1381, 2665
NsiI ATGCAT 3 cut(s) 281, 1485, 2851
NspI RCATGY 2 cut(s) 682, 2456
NspV TTCGAA 1 cut(s) 109
PaeI GCATGC 1 cut(s) 682
PaeR7I CTCGAG 3 cut(s) 196, 708, 2066
PagI TCATGA 1 cut(s) 1567
PaqCI CACCTGC 1 cut(s) 1937
PasI CCCWGGG 1 cut(s) 2014
PceI AGGCCT 1 cut(s) 1734
PciI ACATGT 1 cut(s) 2452
PciSI GCTCTTC 1 cut(s) 1530
PcsI WCGNNNNNNNCGW 1 cut(s) 203
PctI GAATGC 1 cut(s) 2712
PdmI GAANNNNTTC 8 cut(s) 105, 587, 601, 813, 1083, 1515, 1609, 2991
PflFI GACNNNGTC 1 cut(s) 3399
PflMI CCANNNNNTGG 2 cut(s) 1624, 3148
PfoI TCCNGGA 1 cut(s) 3007
PkrI GCNGC 6 cut(s) 184, 342, 457, 645, 874, 1654
PleI GAGTC 5 cut(s) 188, 1755, 2079, 3298, 3431
PpsI GAGTC 5 cut(s) 188, 1755, 2079, 3298, 3431
PscI ACATGT 1 cut(s) 2452
PshBI ATTAAT 1 cut(s) 2111
Psp124BI GAGCTC 1 cut(s) 366
Psp1406I AACGTT 1 cut(s) 2170
Psp6I CCWGG 5 cut(s) 982, 1540, 2013, 3007, 3282
PspEI GGTNACC 1 cut(s) 2665
PspFI CCCAGC 2 cut(s) 486, 1506
PspGI CCWGG 5 cut(s) 982, 1540, 2013, 3007, 3282
PspN4I GGNNCC 4 cut(s) 327, 344, 671, 1204
PspPI GGNCC 4 cut(s) 292, 980, 1720, 3395
PspXI VCTCGAGB 1 cut(s) 708
PstI CTGCAG 1 cut(s) 2270
PstNI CAGNNNCTG 1 cut(s) 2014
PsuI RGATCY 9 cut(s) 142, 325, 669, 1012, 1141, 1445, 1939, 1972, 2495
PsyI GACNNNGTC 1 cut(s) 3399
RsaI GTAC 4 cut(s) 2824, 3078, 3129, 3340
RsaNI GTAC 4 cut(s) 2823, 3077, 3128, 3339
RseI CAYNNNNRTG 4 cut(s) 596, 1628, 2423, 3042
SacI GAGCTC 1 cut(s) 366
SapI GCTCTTC 1 cut(s) 1530
SatI GCNGC 6 cut(s) 183, 341, 456, 644, 873, 1653
Sau96I GGNCC 4 cut(s) 292, 980, 1720, 3395
SchI GAGTC 5 cut(s) 188, 1755, 2080, 3299, 3432
ScrFI CCNGG 6 cut(s) 984, 1542, 2015, 3009, 3284, 3399
SduI GDGCHC 3 cut(s) 366, 1265, 3141
SfaNI GCATC 7 cut(s) 1033, 1297, 2327, 2468, 2836, 2858, 2912
SfcI CTRYAG 2 cut(s) 2266, 3358
Sfr274I CTCGAG 3 cut(s) 196, 708, 2066
SfuI TTCGAA 1 cut(s) 109
SinI GGWCC 3 cut(s) 980, 1720, 3395
SlaI CTCGAG 3 cut(s) 196, 708, 2066
SmiMI CAYNNNNRTG 4 cut(s) 596, 1628, 2423, 3042
SmlI CTYRAG 8 cut(s) 146, 196, 708, 1756, 1961, 2066, 2732, 3371
SmoI CTYRAG 8 cut(s) 146, 196, 708, 1756, 1961, 2066, 2732, 3371
SphI GCATGC 1 cut(s) 682
SseBI AGGCCT 1 cut(s) 1734
SsiI CCGC 7 cut(s) 340, 607, 641, 682, 889, 1652, 2560
SspI AATATT 2 cut(s) 1335, 3019
SspMI CTAG 4 cut(s) 794, 990, 1394, 1409
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 1734
StyD4I CCNGG 6 cut(s) 982, 1540, 2013, 3007, 3282, 3397
StyI CCWWGG 2 cut(s) 673, 1618
TaaI ACNGT 6 cut(s) 1073, 1476, 1527, 2384, 3092, 3359
TaiI ACGT 4 cut(s) 172, 1290, 2173, 2594
TaqI TCGA 6 cut(s) 109, 197, 709, 2067, 2190, 2476
TatI WGTACW 3 cut(s) 2822, 3076, 3127
TauI GCSGC 2 cut(s) 343, 1655
TscAI CASTG 5 cut(s) 2835, 3529, 3535, 3631, 3637
TseFI GTSAC 2 cut(s) 1381, 2665
TseI GCWGC 4 cut(s) 182, 455, 643, 872
Tsp45I GTSAC 2 cut(s) 1381, 2665
TspGWI ACGGA 4 cut(s) 547, 1170, 2302, 3416
TspRI CASTG 5 cut(s) 2835, 3529, 3535, 3631, 3637
Tth111I GACNNNGTC 1 cut(s) 3399
Van91I CCANNNNNTGG 2 cut(s) 1624, 3148
VpaK11BI GGWCC 3 cut(s) 980, 1720, 3395
VspI ATTAAT 1 cut(s) 2111
XapI RAATTY 9 cut(s) 117, 601, 1681, 1802, 1879, 2642, 2854, 3170, 3275
XceI RCATGY 2 cut(s) 682, 2456
XcmI CCANNNNNNNNNTGG 2 cut(s) 829, 3044
XhoI CTCGAG 3 cut(s) 196, 708, 2066
XmiI GTMKAC 2 cut(s) 563, 2797
XmnI GAANNNNTTC 8 cut(s) 105, 587, 601, 813, 1083, 1515, 1609, 2991
XspI CTAG 4 cut(s) 794, 990, 1394, 1409
Zsp2I ATGCAT 3 cut(s) 281, 1485, 2851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.