RLG00000030331

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000105
Physical Location & Seq
Reverse (-)
59706728 .. 59712896
6169 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030331

Sequence Viewer

Length: 2778 bp
ATGGCGTCAACCTCTTCTCGTCGTCGTCAATGGAAATACGATGTGTTTCTAAGTTTCAGGGGGAAGGATACCCGCTACAATTTTACCGGTAGCCTGTATGCTGCACTAAATCGGAAAGGAATTCTCACCTTTATGGATGACCGAGAGCTTCAGAAAGGAAAATCCATTAGCTCTGAACTTTTCGCTGCAATTGAGGAGTCCAGATGTGCGATTGTCATTCTCTCAGAGAACTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGATTGGCCAACAAGTCTTCCCCGTCTTCCGCTGGGTGGATCCTTCTGATGTGCGGCACCAAAGGGGAAGATATGAACGAATACTAATTGAGGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTCATGATTTATTAAGCAATTACATATGTCCACACCTTCTTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGGATAGGTAAGACAACACTTGCTCGAGCTATCCATGATGAAATCCGTCAGGATTTTGACCGAGCCTACTTTCTTTCCAACGTTAGAGAAAGGTCTAAAAACAATGGCCTAGTTTCTCTTCAAGAAAAACTTCTTTCTGAAATCCTAATGGCAAAAATTAAAAATATAGAGCATGAAGACACAGGAGCTGCTATGATAAAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAGAATTGTCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGGTGTTGATGCTACATACAAGGCTAATATGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATGTTCCCCACCAGAAGATTATTTGCATCTGTGCTACGATATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCGCTGATAAATGGGCAAGTGCAATTGATAGGTTAAACAAAACACCAAACAAACACATTATTGGGGTGCTTCGGATTAGTTTTGATGGACTTGATGAAAAAGACCAAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTACTGCCAGCTAGACCCTGTCATTGGTTTAGATGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAACGAACTGTCAATGCATGATTTGCTACAAGAAATGGGCCGGGAAATTGTTCGTGAAGAGTCTCCTAAAGAGCCAGGCAAACGTAGTAGCTTATGGTCTCATGAAGACATCAACAACGTGCTGAAGAGACATAAGAATTTTGATAAGTTGAAGTTCATCAACCTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCACAGGCGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGAGTCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAGAGACTGCAAAAGTCTTGAGAGTCTGCCCAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCCAACTGCTCAAAAATTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTTGATGAGACTGCCATTGAGGAACTGTCGGTTTCAATTGAACAGCTGACTGGCCTTGTGTCATTGAATCTAAGGAACTGCAGAAATCTTGTATGTCTTCCAAGCGCCATCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAGACATCAGGTAAATGTGGGGGAGATGGACTGTTTTGAGGAAATTGATGCAAAGAGTGGGTCTGCAATAGAAATCTCATCTACCCATGATTGCATAAAATATGTGAGAAGTTCAATCTTTCAGGGATGTGAAGCAGTTTGGAGATCTTTAAATAAGTTCTTGCCTACTGGATTGGAGCCAAAAGTGAAAACAGAGCCAATGAGTTTCCGCTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTTGGTTACATTCCCTCTTTGGTGACCTTGAATCTAAGAGGAAACAATTTTGTTCACCTTCCTTCAGGCATTGGATTGCTTTCTAAGCTGGAGAACTTGAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGTTGGTTGTACTTCACTGAAATACTTGTTTGATGCATCATATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCGTTCAAGGATGCAATAACATAGCATTTAAAATGCTGAAGACATTCATGTGTCAGGCAATCTCTAAAAAGGGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTAAATGGTTCCGGCACTGGAGTGTTTCGCAGTCCTTAAGTGTATATCTACCTGCACATTGGAATAACAACGGTGGATTTCTTATAGGTCCAAGGTTGCACCTGGAATTAGTTGAACGCATGCTCGAGTTTGCTGATGACAGGTTGTTCACTAGACCATTTGGCGGTGTCCAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

926

Amino Acids

104.71

Weight (kDa)

8.21

Isoelectric Point (pI)

44.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 12 - 123 1.1e-39 TIR domain
TIR_2 PF13676 15 - 107 2.1e-15 TIR domain
NB-ARC PF00931 169 - 323 8.7e-20 NB-ARC domain
NACHT PF05729 173 - 270 5.9e-07 NACHT domain
WHD_ROQ1 PF23282 390 - 461 6.3e-24 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 498 - 591 7.8e-07 Leucine-rich repeat region
LRR_13 PF23286 558 - 642 5.3e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2659
AccB1I GGYRCC 1 cut(s) 345
AccI GTMKAC 2 cut(s) 416, 2374
AciI CCGC 8 cut(s) 73, 319, 343, 460, 535, 742, 2137, 2763
AclI AACGTT 2 cut(s) 618, 1747
AclWI GGATC 6 cut(s) 323, 336, 517, 530, 792, 1236
AcoI YGGCCR 1 cut(s) 295
AcsI RAATTY 5 cut(s) 120, 1456, 1567, 2219, 2772
AcuI CTGAAG 4 cut(s) 134, 1463, 2268, 2546
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 890
AfaI GTAC 1 cut(s) 2401
AfeI AGCGCT 1 cut(s) 1078
AfiI CCNNNNNNNGG 5 cut(s) 325, 505, 1274, 2150, 2762
AflII CTTAAG 1 cut(s) 2635
AgeI ACCGGT 1 cut(s) 86
AjnI CCWGG 4 cut(s) 835, 1393, 2584, 2700
AjuI GAANNNNNNNTTGG 2 cut(s) 2306, 2338
AleI CACNNNNGTG 1 cut(s) 2619
AluBI AGCT 9 cut(s) 148, 171, 566, 725, 1261, 1410, 1609, 1810, 2308
AluI AGCT 9 cut(s) 148, 171, 566, 725, 1261, 1410, 1609, 1810, 2308
Alw26I GTCTC 7 cut(s) 1386, 1422, 1441, 1502, 1623, 1766, 2332
AlwI GGATC 6 cut(s) 323, 336, 517, 530, 792, 1236
AlwNI CAGNNNCTG 1 cut(s) 725
Ama87I CYCGRG 3 cut(s) 561, 1724, 2723
Aor51HI AGCGCT 1 cut(s) 1078
AoxI GGCC 4 cut(s) 295, 643, 1357, 1816
ApeKI GCWGC 3 cut(s) 101, 185, 725
ApoI RAATTY 5 cut(s) 120, 1456, 1567, 2219, 2772
ArsI GACNNNNNNTTYG 2 cut(s) 1973, 2005
AseI ATTAAT 1 cut(s) 1688
AsiGI ACCGGT 1 cut(s) 86
Asp700I GAANNNNTTC 6 cut(s) 454, 666, 936, 1368, 2531, 2568
AspLEI GCGC 2 cut(s) 1079, 1871
AspS9I GGNCC 3 cut(s) 833, 1357, 2687
AsuC2I CCSGG 1 cut(s) 1361
AsuHPI GGTGA 4 cut(s) 118, 2210, 2254, 2267
AvaI CYCGRG 3 cut(s) 561, 1724, 2723
AvaII GGWCC 2 cut(s) 833, 2687
BalI TGGCCA 1 cut(s) 297
BamHI GGATCC 2 cut(s) 328, 522
BanI GGYRCC 1 cut(s) 345
BauI CACGAG 1 cut(s) 1043
BbsI GAAGAC 6 cut(s) 298, 307, 720, 1431, 1853, 2534
BbvI GCAGC 3 cut(s) 88, 172, 712
BccI CCATC 5 cut(s) 1154, 1590, 1880, 1948, 2371
BciT130I CCWGG 4 cut(s) 837, 1395, 2586, 2702
BciVI GTATCC 1 cut(s) 61
BcnI CCSGG 1 cut(s) 1361
BcoDI GTCTC 7 cut(s) 1386, 1422, 1441, 1502, 1623, 1766, 2332
BfaI CTAG 5 cut(s) 647, 1247, 1262, 2378, 2751
BfmI CTRYAG 1 cut(s) 1843
BfoI RGCGCY 2 cut(s) 1080, 1872
BfrI CTTAAG 1 cut(s) 2635
BfuAI ACCTGC 1 cut(s) 2659
BfuI GTATCC 1 cut(s) 61
BglII AGATCT 3 cut(s) 1298, 1549, 2072
BisI GCNGC 4 cut(s) 102, 186, 344, 726
BlsI GCNGC 4 cut(s) 103, 187, 345, 727
Bme1390I CCNGG 5 cut(s) 837, 1361, 1395, 2586, 2702
Bme18I GGWCC 2 cut(s) 833, 2687
BmeT110I CYCGRG 3 cut(s) 561, 1724, 2723
BmgT120I GGNCC 3 cut(s) 833, 1357, 2687
BmiI GGNNCC 5 cut(s) 330, 347, 524, 2106, 2609
BmrFI CCNGG 5 cut(s) 837, 1361, 1395, 2586, 2702
BmrI ACTGGG 1 cut(s) 1650
BmsI GCATC 7 cut(s) 886, 1003, 1904, 1966, 2413, 2435, 2489
BmuI ACTGGG 1 cut(s) 1650
BpiI GAAGAC 6 cut(s) 298, 307, 720, 1431, 1853, 2534
BpmI CTGGAG 2 cut(s) 2330, 2638
Bpu10I CCTNAGC 1 cut(s) 1023
BpuEI CTTGAG 2 cut(s) 1559, 1664
BpuMI CCSGG 1 cut(s) 1361
BsaBI GATNNNNATC 2 cut(s) 783, 1227
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1422, 1502
BsaJI CCNNGG 4 cut(s) 526, 836, 1591, 2690
BsaWI WCCGGW 1 cut(s) 86
Bsc4I CCNNNNNNNGG 5 cut(s) 325, 505, 1274, 2150, 2762
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 5 cut(s) 830, 1656, 1819, 2101, 2621
Bse8I GATNNNNATC 2 cut(s) 783, 1227
BseBI CCWGG 4 cut(s) 837, 1395, 2586, 2702
BseDI CCNNGG 4 cut(s) 526, 836, 1591, 2690
BseGI GGATG 6 cut(s) 142, 262, 1565, 1919, 2060, 2504
BseJI GATNNNNATC 2 cut(s) 783, 1227
BseLI CCNNNNNNNGG 5 cut(s) 325, 505, 1274, 2150, 2762
BseMII CTCAG 3 cut(s) 237, 1037, 2171
BseNI ACTGG 5 cut(s) 830, 1656, 1819, 2101, 2621
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 3 cut(s) 88, 172, 712
BseYI CCCAGC 1 cut(s) 321
BsgI GTGCAG 2 cut(s) 87, 2637
BshFI GGCC 4 cut(s) 297, 645, 1359, 1818
BshNI GGYRCC 1 cut(s) 345
BshTI ACCGGT 1 cut(s) 86
BsiHKCI CYCGRG 3 cut(s) 561, 1724, 2723
BsiSI CCGG 3 cut(s) 87, 1360, 2611
BslI CCNNNNNNNGG 5 cut(s) 325, 505, 1274, 2150, 2762
BsmAI GTCTC 7 cut(s) 1386, 1422, 1441, 1502, 1623, 1766, 2332
BsmI GAATGC 1 cut(s) 462
BsnI GGCC 4 cut(s) 297, 645, 1359, 1818
Bso31I GGTCTC 2 cut(s) 1422, 1502
BsoBI CYCGRG 3 cut(s) 561, 1724, 2723
Bsp143I GATC 7 cut(s) 328, 522, 784, 1228, 1298, 1549, 2072
Bsp19I CCATGG 1 cut(s) 526
BspACI CCGC 8 cut(s) 73, 319, 343, 460, 535, 742, 2137, 2763
BspANI GGCC 4 cut(s) 297, 645, 1359, 1818
BspCNI CTCAG 3 cut(s) 236, 1036, 2172
BspHI TCATGA 2 cut(s) 466, 1420
BspLI GGNNCC 5 cut(s) 330, 347, 524, 2106, 2609
BspMAI CTGCAG 1 cut(s) 1847
BspMI ACCTGC 1 cut(s) 2659
BspPI GGATC 6 cut(s) 323, 336, 517, 530, 792, 1236
BspT107I GGYRCC 1 cut(s) 345
BspTI CTTAAG 1 cut(s) 2635
BspTNI GGTCTC 2 cut(s) 1422, 1502
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 5 cut(s) 830, 1656, 1819, 2101, 2621
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 4 cut(s) 526, 836, 1591, 2690
BssMI GATC 7 cut(s) 328, 522, 784, 1228, 1298, 1549, 2072
BssNAI GTATAC 2 cut(s) 417, 2375
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1043
BssT1I CCWWGG 3 cut(s) 526, 1591, 2690
Bst1107I GTATAC 2 cut(s) 417, 2375
Bst2BI CACGAG 1 cut(s) 1043
Bst2UI CCWGG 4 cut(s) 837, 1395, 2586, 2702
Bst4CI ACNGT 5 cut(s) 926, 1329, 1791, 1961, 2672
Bst6I CTCTTC 4 cut(s) 19, 660, 1371, 1439
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 2635
BstAPI GCANNNNNTGC 1 cut(s) 1342
BstC8I GCNNGC 5 cut(s) 533, 1204, 1259, 2141, 2720
BstDEI CTNAG 8 cut(s) 50, 223, 1023, 1625, 1835, 2180, 2255, 2304
BstDSI CCRYGG 1 cut(s) 526
BstEII GGTNACC 1 cut(s) 2242
BstF5I GGATG 6 cut(s) 142, 262, 1565, 1919, 2060, 2504
BstH2I RGCGCY 2 cut(s) 1080, 1872
BstHHI GCGC 2 cut(s) 1079, 1871
BstKTI GATC 7 cut(s) 331, 525, 787, 1231, 1301, 1552, 2075
BstMAI GTCTC 7 cut(s) 1386, 1422, 1441, 1502, 1623, 1766, 2332
BstMBI GATC 7 cut(s) 328, 522, 784, 1228, 1298, 1549, 2072
BstMWI GCNNNNNNNGC 2 cut(s) 1342, 2305
BstNI CCWGG 4 cut(s) 837, 1395, 2586, 2702
BstNSI RCATGY 2 cut(s) 535, 2722
BstPI GGTNACC 1 cut(s) 2242
BstSCI CCNGG 5 cut(s) 835, 1359, 1393, 2584, 2700
BstSFI CTRYAG 1 cut(s) 1843
BstV1I GCAGC 3 cut(s) 88, 172, 712
BstV2I GAAGAC 6 cut(s) 298, 307, 720, 1431, 1853, 2534
BstX2I RGATCY 5 cut(s) 328, 522, 1298, 1549, 2072
BstYI RGATCY 5 cut(s) 328, 522, 1298, 1549, 2072
BstZ17I GTATAC 2 cut(s) 417, 2375
BsuI GTATCC 1 cut(s) 61
BsuRI GGCC 4 cut(s) 297, 645, 1359, 1818
BtgI CCRYGG 1 cut(s) 526
BtsCI GGATG 6 cut(s) 142, 262, 1565, 1919, 2060, 2504
BtsIMutI CAGTG 2 cut(s) 2405, 2614
BveI ACCTGC 1 cut(s) 2659
Cac8I GCNNGC 5 cut(s) 533, 1204, 1259, 2141, 2720
CaiI CAGNNNCTG 1 cut(s) 725
CciI TCATGA 2 cut(s) 466, 1420
CfoI GCGC 2 cut(s) 1079, 1871
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 3 cut(s) 833, 1357, 2687
Csp6I GTAC 1 cut(s) 2400
CspAI ACCGGT 1 cut(s) 86
CviQI GTAC 1 cut(s) 2400
DdeI CTNAG 8 cut(s) 50, 223, 1023, 1625, 1835, 2180, 2255, 2304
DpnI GATC 7 cut(s) 330, 524, 786, 1230, 1300, 1551, 2074
DpnII GATC 7 cut(s) 328, 522, 784, 1228, 1298, 1549, 2072
DraI TTTAAA 2 cut(s) 2079, 2518
DraIII CACNNNGTG 1 cut(s) 890
EaeI YGGCCR 1 cut(s) 295
Eam1104I CTCTTC 4 cut(s) 19, 660, 1371, 1439
EarI CTCTTC 4 cut(s) 19, 660, 1371, 1439
Eco130I CCWWGG 3 cut(s) 526, 1591, 2690
Eco31I GGTCTC 2 cut(s) 1422, 1502
Eco47I GGWCC 2 cut(s) 833, 2687
Eco47III AGCGCT 1 cut(s) 1078
Eco57I CTGAAG 4 cut(s) 134, 1463, 2268, 2546
Eco88I CYCGRG 3 cut(s) 561, 1724, 2723
Eco91I GGTNACC 1 cut(s) 2242
EcoO65I GGTNACC 1 cut(s) 2242
EcoRI GAATTC 1 cut(s) 120
EcoRII CCWGG 4 cut(s) 835, 1393, 2584, 2700
EcoT14I CCWWGG 3 cut(s) 526, 1591, 2690
EcoT22I ATGCAT 3 cut(s) 284, 1338, 2428
ErhI CCWWGG 3 cut(s) 526, 1591, 2690
FalI AAGNNNNNCTT 2 cut(s) 651, 683
FauI CCCGC 3 cut(s) 80, 467, 528
FauNDI CATATG 1 cut(s) 488
FblI GTMKAC 2 cut(s) 416, 2374
Fnu4HI GCNGC 4 cut(s) 102, 186, 344, 726
FokI GGATG 6 cut(s) 149, 269, 1572, 1926, 2067, 2511
Fsp4HI GCNGC 4 cut(s) 102, 186, 344, 726
FspBI CTAG 5 cut(s) 647, 1247, 1262, 2378, 2751
GlaI GCGC 2 cut(s) 1078, 1870
GluI GCNGC 4 cut(s) 102, 186, 344, 726
GsaI CCCAGC 1 cut(s) 325
GsuI CTGGAG 2 cut(s) 2330, 2638
HaeII RGCGCY 2 cut(s) 1080, 1872
HaeIII GGCC 4 cut(s) 297, 645, 1359, 1818
HapII CCGG 3 cut(s) 87, 1360, 2611
HhaI GCGC 2 cut(s) 1079, 1871
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 1077, 1869
HinP1I GCGC 2 cut(s) 1077, 1869
HincII GTYRAC 3 cut(s) 9, 433, 792
HindII GTYRAC 3 cut(s) 9, 433, 792
HindIII AAGCTT 1 cut(s) 1607
HpaII CCGG 3 cut(s) 87, 1360, 2611
HphI GGTGA 4 cut(s) 118, 2210, 2254, 2267
Hpy166II GTNNAC 8 cut(s) 9, 417, 433, 494, 792, 2275, 2375, 2748
Hpy8I GTNNAC 8 cut(s) 9, 417, 433, 494, 792, 2275, 2375, 2748
Hpy99I CGWCG 2 cut(s) 24, 27
HpyCH4III ACNGT 5 cut(s) 926, 1329, 1791, 1961, 2672
HpyCH4IV ACGT 4 cut(s) 618, 1402, 1437, 1747
HpyF10VI GCNNNNNNNGC 2 cut(s) 1342, 2305
HpyF3I CTNAG 8 cut(s) 50, 223, 1023, 1625, 1835, 2180, 2255, 2304
HpySE526I ACGT 4 cut(s) 618, 1402, 1437, 1747
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 1077, 1869
Kzo9I GATC 7 cut(s) 328, 522, 784, 1228, 1298, 1549, 2072
LmnI GCTCC 3 cut(s) 722, 2104, 2204
Lsp1109I GCAGC 3 cut(s) 88, 172, 712
LweI GCATC 7 cut(s) 886, 1003, 1904, 1966, 2413, 2435, 2489
MaeI CTAG 5 cut(s) 647, 1247, 1262, 2378, 2751
MaeII ACGT 4 cut(s) 618, 1402, 1437, 1747
MaeIII GTNAC 3 cut(s) 1234, 2225, 2242
MalI GATC 7 cut(s) 330, 524, 786, 1230, 1300, 1551, 2074
MboI GATC 7 cut(s) 328, 522, 784, 1228, 1298, 1549, 2072
MfeI CAATTG 4 cut(s) 189, 1098, 1800, 2470
MflI RGATCY 5 cut(s) 328, 522, 1298, 1549, 2072
MlsI TGGCCA 1 cut(s) 297
MluNI TGGCCA 1 cut(s) 297
MlyI GAGTC 4 cut(s) 206, 1388, 1587, 1657
MmeI TCCRAC 4 cut(s) 639, 1341, 1714, 1722
MnlI CCTC 9 cut(s) 22, 187, 373, 1493, 1529, 1777, 1960, 2245, 2252
Mox20I TGGCCA 1 cut(s) 297
Mph1103I ATGCAT 3 cut(s) 284, 1338, 2428
MroXI GAANNNNTTC 6 cut(s) 454, 666, 936, 1368, 2531, 2568
MscI TGGCCA 1 cut(s) 297
MslI CAYNNNNRTG 4 cut(s) 131, 449, 2536, 2619
Msp20I TGGCCA 1 cut(s) 297
MspA1I CMGCKG 2 cut(s) 321, 1810
MspCI CTTAAG 1 cut(s) 2635
MspI CCGG 3 cut(s) 87, 1360, 2611
MspR9I CCNGG 5 cut(s) 837, 1361, 1395, 2586, 2702
MunI CAATTG 4 cut(s) 189, 1098, 1800, 2470
Mva1269I GAATGC 1 cut(s) 462
MvaI CCWGG 4 cut(s) 837, 1395, 2586, 2702
MwoI GCNNNNNNNGC 2 cut(s) 1342, 2305
NciI CCSGG 1 cut(s) 1361
NcoI CCATGG 1 cut(s) 526
NdeI CATATG 1 cut(s) 488
NdeII GATC 7 cut(s) 328, 522, 784, 1228, 1298, 1549, 2072
NlaIV GGNNCC 5 cut(s) 330, 347, 524, 2106, 2609
NmeAIII GCCGAG 1 cut(s) 1905
NmuCI GTSAC 2 cut(s) 1234, 2242
NsiI ATGCAT 3 cut(s) 284, 1338, 2428
NspI RCATGY 2 cut(s) 535, 2722
OliI CACNNNNGTG 1 cut(s) 2619
PaeI GCATGC 2 cut(s) 535, 2722
PaeR7I CTCGAG 2 cut(s) 561, 2723
PagI TCATGA 2 cut(s) 466, 1420
PctI GAATGC 1 cut(s) 462
PdmI GAANNNNTTC 6 cut(s) 454, 666, 936, 1368, 2531, 2568
PfeI GAWTC 7 cut(s) 455, 1534, 1621, 1673, 1720, 1831, 2251
PflFI GACNNNGTC 1 cut(s) 1268
PfoI TCCNGGA 1 cut(s) 2584
PinAI ACCGGT 1 cut(s) 86
PkrI GCNGC 4 cut(s) 103, 187, 345, 727
PleI GAGTC 4 cut(s) 205, 1387, 1586, 1656
PpsI GAGTC 4 cut(s) 205, 1387, 1586, 1656
PshBI ATTAAT 1 cut(s) 1688
Psp1406I AACGTT 2 cut(s) 618, 1747
Psp6I CCWGG 4 cut(s) 835, 1393, 2584, 2700
PspEI GGTNACC 1 cut(s) 2242
PspFI CCCAGC 1 cut(s) 321
PspGI CCWGG 4 cut(s) 835, 1393, 2584, 2700
PspN4I GGNNCC 5 cut(s) 330, 347, 524, 2106, 2609
PspPI GGNCC 3 cut(s) 833, 1357, 2687
PspXI VCTCGAGB 2 cut(s) 561, 2723
PstI CTGCAG 1 cut(s) 1847
PstNI CAGNNNCTG 1 cut(s) 725
PsuI RGATCY 5 cut(s) 328, 522, 1298, 1549, 2072
PsyI GACNNNGTC 1 cut(s) 1268
PvuII CAGCTG 1 cut(s) 1810
RsaI GTAC 1 cut(s) 2401
RsaNI GTAC 1 cut(s) 2400
RseI CAYNNNNRTG 4 cut(s) 131, 449, 2536, 2619
SatI GCNGC 4 cut(s) 102, 186, 344, 726
Sau3AI GATC 7 cut(s) 328, 522, 784, 1228, 1298, 1549, 2072
Sau96I GGNCC 3 cut(s) 833, 1357, 2687
SchI GAGTC 4 cut(s) 206, 1388, 1587, 1657
ScrFI CCNGG 5 cut(s) 837, 1361, 1395, 2586, 2702
SfaNI GCATC 7 cut(s) 886, 1003, 1904, 1966, 2413, 2435, 2489
SfcI CTRYAG 1 cut(s) 1843
Sfr274I CTCGAG 2 cut(s) 561, 2723
SinI GGWCC 2 cut(s) 833, 2687
SlaI CTCGAG 2 cut(s) 561, 2723
SmiMI CAYNNNNRTG 4 cut(s) 131, 449, 2536, 2619
SmlI CTYRAG 5 cut(s) 561, 1538, 1643, 2635, 2723
SmoI CTYRAG 5 cut(s) 561, 1538, 1643, 2635, 2723
SphI GCATGC 2 cut(s) 535, 2722
SsiI CCGC 8 cut(s) 73, 319, 343, 460, 535, 742, 2137, 2763
SspI AATATT 2 cut(s) 1188, 2596
SspMI CTAG 5 cut(s) 647, 1247, 1262, 2378, 2751
StyD4I CCNGG 5 cut(s) 835, 1359, 1393, 2584, 2700
StyI CCWWGG 3 cut(s) 526, 1591, 2690
TaaI ACNGT 5 cut(s) 926, 1329, 1791, 1961, 2672
TaiI ACGT 4 cut(s) 621, 1405, 1440, 1750
TaqI TCGA 2 cut(s) 562, 2724
TaqII GACCGA 2 cut(s) 156, 612
TatI WGTACW 1 cut(s) 2399
TauI GCSGC 1 cut(s) 346
TfiI GAWTC 7 cut(s) 455, 1534, 1621, 1673, 1720, 1831, 2251
TscAI CASTG 2 cut(s) 2412, 2621
TseFI GTSAC 2 cut(s) 1234, 2242
TseI GCWGC 3 cut(s) 101, 185, 725
Tsp45I GTSAC 2 cut(s) 1234, 2242
TspGWI ACGGA 3 cut(s) 400, 572, 1023
TspRI CASTG 2 cut(s) 2412, 2621
Tth111I GACNNNGTC 1 cut(s) 1268
Vha464I CTTAAG 1 cut(s) 2635
VpaK11BI GGWCC 2 cut(s) 833, 2687
VspI ATTAAT 1 cut(s) 1688
XapI RAATTY 5 cut(s) 120, 1456, 1567, 2219, 2772
XceI RCATGY 2 cut(s) 535, 2722
XcmI CCANNNNNNNNNTGG 1 cut(s) 1589
XhoI CTCGAG 2 cut(s) 561, 2723
XmiI GTMKAC 2 cut(s) 416, 2374
XmnI GAANNNNTTC 6 cut(s) 454, 666, 936, 1368, 2531, 2568
XspI CTAG 5 cut(s) 647, 1247, 1262, 2378, 2751
Zsp2I ATGCAT 3 cut(s) 284, 1338, 2428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.