Rw6G002220

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000105
Physical Location & Seq
Forward (+)
3574061 .. 3576050
1990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002220.1

Sequence Viewer

Length: 1809 bp
ATGGTAATGGAGTGGTCTGAATTAGAAGTGGCTCATTGGAATCCAGAAGCCTTGTCAAATTTGTCTCAACTTATTCTTCTCCATATTCATAATGTGGACCTTCCTAAAGGCCTTACCTGTCTTCCTAATTCCTTGAGACTCCTGGAATGGCCTGGCTGTCCGTTAAGATCTCTCCCACGAACGTTCGAACCAGATGAACTTATTGAACTTAACTTGTGTCACAGCAATATTGAACATCTTTGGAAGGGAACAAAGAATTTTAACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGATCCCAGACCTTGCAGGTGTTCAGAATCTTGAGAGTTTAGAGCTCGAAGGATGTAAGAGTTTGGTAAGAATCCATCAATCCCTTGGATGTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTTGAGAATCTGCCAGGTAGAATTGAAATGGAATCTCTTGAAAAATTAATTCTTTCTAACTGCTCCAAAATTAAAAAGATTCCCGAGTTTGATGGAAATATGGAGCGTCTGTCTGTGCTTCATCTTGACGGGACTGCCATTGAGGGACTGCCTGTTTCAATTGGACGCCTGAGTGGCCTTGTGTCATTGAATCTAAGTAAGTGCAGAAATCTTGTTTGTCTTCCAAGCACCATCGTTCATTTATGCAAAGTAGTTTGGGGATCTTTAAATAAGTTCTTGCCTTCTGGATTGCAAAAAGTGAATGGAGAGCCAATGAGTTTCAGCTTGCCTAGGCTGTGTAATTTAACAGATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAATGAATTCAGTTACTTTCCCTCTTTGGTGACCTTGAATATAAGTGGAAACAATTTTGTTCGTCTTCCTTCAGGCATTGGGTCGCTTTCCAAGCTTGAGTACTGTAAGTTGAAGAATTGCAAGAGACTTGAAGATCTGTCAGACCTTCCATTGAATAGTAACCTAGTTTTAAAGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTTAACAGATTAAACGAATCATGTTTTGGTTCAGGTTTCAATTTCATGAATTGCGTCAATCTAAAGGGCAATCAAGGATGCAATAGCATGGCATTTGAAATGCTGAAGGCAGTCATGTATCAGGGACTCTCTAGTGAGACGAAAACTATTCGAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCGAAGTGTTGGGTGTTCAGTATGTGTAGAGCTGCCTCCAGATTGGGATAGCAGTAGGTTTATGGGATTTGCTTTGTGTGTTGTTTTTGTACTCCATGAGAACCATCGTCTTAAGCGATTCAAGACTTTTAACGATGATCATTATCTTTCCTGTAGCATGAAGCTCAATGGAGAAGAATTCGCTGGAAATATCCGAGAGCCTTTATTTTGCTTTCGTGACGAAGTTTGCCCGTTTGAGTTGGATCACCTGTGGCTATTGTATGTATCTCGTGATGGATACTTTGGTAGTACAGGGTGGTGGCATAACATTTGTGGTCAGCTTAATTTCTTATTTCATACCAATGAGCTGTATCCGAATGGGAATAAGTGTGAAGTCCGTCTGTGTGGAGTCCGTCTGATATATGAGCAAGACGTGCAAGAGTTGAACAAACAAACAACTCAATCAAGCAGGAGGATGTCTCCAGATGAGGATATATTGATTGATTTAGACATTGCAGTTGATGGGGAAACTAGTGGCACTGGTAGGTGTGGTAGCAGAACTTGCACGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

602

Amino Acids

67.8

Weight (kDa)

6.01

Isoelectric Point (pI)

42.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_13 PF23286 158 - 325 3.8e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 405 - 555 6.3e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 314
Acc36I ACCTGC 1 cut(s) 314
AclI AACGTT 1 cut(s) 182
AclWI GGATC 3 cut(s) 304, 709, 1497
AcsI RAATTY 5 cut(s) 58, 256, 839, 1051, 1424
AcuI CTGAAG 2 cut(s) 888, 1178
AcyI GRCGYC 1 cut(s) 607
AfaI GTAC 4 cut(s) 935, 1021, 1338, 1537
AflII CTTAAG 1 cut(s) 1358
AhlI ACTAGT 1 cut(s) 1757
AjiI CACGTC 1 cut(s) 1660
AjnI CCWGG 4 cut(s) 141, 151, 454, 1216
AjuI GAANNNNNNNTTGG 2 cut(s) 1062, 1094
AluBI AGCT 8 cut(s) 352, 409, 765, 928, 1279, 1411, 1567, 1594
AluI AGCT 8 cut(s) 352, 409, 765, 928, 1279, 1411, 1567, 1594
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 6 cut(s) 69, 130, 404, 952, 1184, 1710
AlwI GGATC 3 cut(s) 304, 709, 1497
Ama87I CYCGRG 1 cut(s) 524
AoxI GGCC 3 cut(s) 109, 149, 616
ApeKI GCWGC 1 cut(s) 1279
ApoI RAATTY 5 cut(s) 58, 256, 839, 1051, 1424
AseI ATTAAT 1 cut(s) 488
AspA2I CCTAGG 1 cut(s) 770
AspS9I GGNCC 1 cut(s) 97
AsuHPI GGTGA 3 cut(s) 830, 874, 1484
AsuII TTCGAA 2 cut(s) 186, 1204
AvaI CYCGRG 1 cut(s) 524
AvaII GGWCC 1 cut(s) 97
AvrII CCTAGG 1 cut(s) 770
BaeI ACNNNNGTAYC 2 cut(s) 1516, 1549
BanII GRGCYC 1 cut(s) 354
BauI CACGAG 1 cut(s) 1515
BbsI GAAGAC 3 cut(s) 113, 653, 890
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 1 cut(s) 1266
BccI CCATC 8 cut(s) 390, 527, 680, 1007, 1254, 1359, 1514, 1742
BciT130I CCWGG 4 cut(s) 143, 153, 456, 1218
BciVI GTATCC 2 cut(s) 1517, 1608
BclI TGATCA 1 cut(s) 1384
BcoDI GTCTC 6 cut(s) 69, 130, 404, 952, 1184, 1710
BcuI ACTAGT 1 cut(s) 1757
BfaI CTAG 4 cut(s) 771, 998, 1185, 1758
BfmI CTRYAG 1 cut(s) 1399
BfrI CTTAAG 1 cut(s) 1358
BfuAI ACCTGC 1 cut(s) 314
BfuI GTATCC 2 cut(s) 1517, 1608
BglI GCCNNNNNGGC 1 cut(s) 615
BglII AGATCT 3 cut(s) 167, 790, 967
BisI GCNGC 1 cut(s) 1280
BlnI CCTAGG 1 cut(s) 770
BlsI GCNGC 1 cut(s) 1281
BmcAI AGTACT 1 cut(s) 935
Bme1390I CCNGG 4 cut(s) 143, 153, 456, 1218
Bme18I GGWCC 1 cut(s) 97
BmeT110I CYCGRG 1 cut(s) 524
BmgBI CACGTC 1 cut(s) 1660
BmgT120I GGNCC 1 cut(s) 97
BmrFI CCNGG 4 cut(s) 143, 153, 456, 1218
BmsI GCATC 3 cut(s) 1033, 1055, 1121
BpiI GAAGAC 3 cut(s) 113, 653, 890
BplI GAGNNNNNCTC 2 cut(s) 1690, 1722
BpmI CTGGAG 2 cut(s) 1269, 1692
Bpu14I TTCGAA 2 cut(s) 186, 1204
BpuEI CTTGAG 4 cut(s) 154, 359, 464, 950
BsaBI GATNNNNATC 1 cut(s) 1389
BsaHI GRCGYC 1 cut(s) 607
BsaJI CCNNGG 2 cut(s) 391, 770
BsaXI ACNNNNNCTCC 2 cut(s) 536, 566
Bse1I ACTGG 1 cut(s) 1771
Bse3DI GCAATG 1 cut(s) 1737
Bse8I GATNNNNATC 1 cut(s) 1389
BseBI CCWGG 4 cut(s) 143, 153, 456, 1218
BseDI CCNNGG 2 cut(s) 391, 770
BseGI GGATG 4 cut(s) 365, 401, 1136, 1707
BseJI GATNNNNATC 1 cut(s) 1389
BseMI GCAATG 1 cut(s) 1737
BseMII CTCAG 3 cut(s) 602, 791, 1224
BseNI ACTGG 1 cut(s) 1771
BseXI GCAGC 1 cut(s) 1266
BsgI GTGCAG 1 cut(s) 664
BshFI GGCC 3 cut(s) 111, 151, 618
BsiHKAI GWGCWC 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 524
BslFI GGGAC 3 cut(s) 586, 600, 1191
BsmAI GTCTC 6 cut(s) 69, 130, 404, 952, 1184, 1710
BsmBI CGTCTC 1 cut(s) 1184
BsmFI GGGAC 3 cut(s) 586, 600, 1191
BsnI GGCC 3 cut(s) 111, 151, 618
BsoBI CYCGRG 1 cut(s) 524
Bsp119I TTCGAA 2 cut(s) 186, 1204
Bsp1286I GDGCHC 1 cut(s) 354
Bsp143I GATC 7 cut(s) 167, 309, 701, 790, 967, 1384, 1489
BspANI GGCC 3 cut(s) 111, 151, 618
BspCNI CTCAG 3 cut(s) 603, 792, 1225
BspHI TCATGA 1 cut(s) 1098
BspMI ACCTGC 1 cut(s) 314
BspPI GGATC 3 cut(s) 304, 709, 1497
BspT104I TTCGAA 2 cut(s) 186, 1204
BspTI CTTAAG 1 cut(s) 1358
BsrDI GCAATG 1 cut(s) 1737
BsrI ACTGG 1 cut(s) 1771
BssECI CCNNGG 2 cut(s) 391, 770
BssMI GATC 7 cut(s) 167, 309, 701, 790, 967, 1384, 1489
BssNI GRCGYC 1 cut(s) 607
BssSI CACGAG 1 cut(s) 1515
BssT1I CCWWGG 2 cut(s) 391, 770
Bst2BI CACGAG 1 cut(s) 1515
Bst2UI CCWGG 4 cut(s) 143, 153, 456, 1218
Bst4CI ACNGT 1 cut(s) 938
BstACI GRCGYC 1 cut(s) 607
BstAFI CTTAAG 1 cut(s) 1358
BstAPI GCANNNNNTGC 2 cut(s) 1660, 1788
BstBI TTCGAA 2 cut(s) 186, 1204
BstC8I GCNNGC 2 cut(s) 767, 1793
BstDEI CTNAG 4 cut(s) 611, 635, 800, 1233
BstEII GGTNACC 1 cut(s) 862
BstF5I GGATG 4 cut(s) 365, 401, 1136, 1707
BstKTI GATC 7 cut(s) 170, 312, 704, 793, 970, 1387, 1492
BstMAI GTCTC 6 cut(s) 69, 130, 404, 952, 1184, 1710
BstMBI GATC 7 cut(s) 167, 309, 701, 790, 967, 1384, 1489
BstMWI GCNNNNNNNGC 4 cut(s) 615, 925, 1660, 1788
BstNI CCWGG 4 cut(s) 143, 153, 456, 1218
BstPI GGTNACC 1 cut(s) 862
BstSCI CCNGG 4 cut(s) 141, 151, 454, 1216
BstSFI CTRYAG 1 cut(s) 1399
BstV1I GCAGC 1 cut(s) 1266
BstV2I GAAGAC 3 cut(s) 113, 653, 890
BstX2I RGATCY 5 cut(s) 167, 309, 701, 790, 967
BstYI RGATCY 5 cut(s) 167, 309, 701, 790, 967
BsuI GTATCC 2 cut(s) 1517, 1608
BsuRI GGCC 3 cut(s) 111, 151, 618
BtrI CACGTC 1 cut(s) 1660
BtsCI GGATG 4 cut(s) 365, 401, 1136, 1707
BtsIMutI CAGTG 2 cut(s) 1025, 1764
BveI ACCTGC 1 cut(s) 314
Cac8I GCNNGC 2 cut(s) 767, 1793
CciI TCATGA 1 cut(s) 1098
Cfr13I GGNCC 1 cut(s) 97
CseI GACGC 3 cut(s) 536, 615, 1096
Csp6I GTAC 4 cut(s) 934, 1020, 1337, 1536
CviAII CATG 6 cut(s) 1074, 1099, 1141, 1168, 1343, 1405
CviQI GTAC 4 cut(s) 934, 1020, 1337, 1536
DdeI CTNAG 4 cut(s) 611, 635, 800, 1233
DpnI GATC 7 cut(s) 169, 311, 703, 792, 969, 1386, 1491
DpnII GATC 7 cut(s) 167, 309, 701, 790, 967, 1384, 1489
DraI TTTAAA 2 cut(s) 708, 1005
Ecl136II GAGCTC 1 cut(s) 352
Eco130I CCWWGG 2 cut(s) 391, 770
Eco147I AGGCCT 1 cut(s) 111
Eco24I GRGCYC 1 cut(s) 354
Eco47I GGWCC 1 cut(s) 97
Eco53kI GAGCTC 1 cut(s) 352
Eco57I CTGAAG 2 cut(s) 888, 1178
Eco88I CYCGRG 1 cut(s) 524
Eco91I GGTNACC 1 cut(s) 862
EcoICRI GAGCTC 1 cut(s) 352
EcoO65I GGTNACC 1 cut(s) 862
EcoRI GAATTC 2 cut(s) 839, 1424
EcoRII CCWGG 4 cut(s) 141, 151, 454, 1216
EcoT14I CCWWGG 2 cut(s) 391, 770
EcoT22I ATGCAT 1 cut(s) 1048
EcoT38I GRGCYC 1 cut(s) 354
ErhI CCWWGG 2 cut(s) 391, 770
Esp3I CGTCTC 1 cut(s) 1184
FaeI CATG 6 cut(s) 1077, 1102, 1144, 1171, 1346, 1408
FaqI GGGAC 3 cut(s) 586, 600, 1191
FatI CATG 6 cut(s) 1073, 1098, 1140, 1167, 1342, 1404
FbaI TGATCA 1 cut(s) 1384
Fnu4HI GCNGC 1 cut(s) 1280
FokI GGATG 4 cut(s) 372, 408, 1143, 1714
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 1 cut(s) 1280
FspBI CTAG 4 cut(s) 771, 998, 1185, 1758
GluI GCNGC 1 cut(s) 1280
GsuI CTGGAG 2 cut(s) 1269, 1692
HaeIII GGCC 3 cut(s) 111, 151, 618
HgaI GACGC 3 cut(s) 536, 615, 1096
Hin1I GRCGYC 1 cut(s) 607
Hin1II CATG 6 cut(s) 1077, 1102, 1144, 1171, 1346, 1408
HindIII AAGCTT 2 cut(s) 407, 926
HphI GGTGA 3 cut(s) 830, 874, 1484
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 7 cut(s) 19, 333, 795, 976, 1442, 1602, 1644
Hpy8I GTNNAC 1 cut(s) 97
HpyAV CCTTC 8 cut(s) 110, 238, 350, 732, 815, 912, 989, 1153
HpyCH4III ACNGT 1 cut(s) 938
HpyCH4IV ACGT 2 cut(s) 182, 1659
HpyF10VI GCNNNNNNNGC 4 cut(s) 615, 925, 1660, 1788
HpyF3I CTNAG 4 cut(s) 611, 635, 800, 1233
HpySE526I ACGT 2 cut(s) 182, 1659
Hsp92I GRCGYC 1 cut(s) 607
Hsp92II CATG 6 cut(s) 1077, 1102, 1144, 1171, 1346, 1408
Ksp22I TGATCA 1 cut(s) 1384
Kzo9I GATC 7 cut(s) 167, 309, 701, 790, 967, 1384, 1489
LmnI GCTCC 3 cut(s) 509, 544, 824
Lsp1109I GCAGC 1 cut(s) 1266
LweI GCATC 3 cut(s) 1033, 1055, 1121
MaeI CTAG 4 cut(s) 771, 998, 1185, 1758
MaeII ACGT 2 cut(s) 182, 1659
MaeIII GTNAC 5 cut(s) 218, 845, 862, 992, 1463
MalI GATC 7 cut(s) 169, 311, 703, 792, 969, 1386, 1491
MboI GATC 7 cut(s) 167, 309, 701, 790, 967, 1384, 1489
MboII GAAGA 7 cut(s) 68, 113, 653, 890, 958, 977, 1433
MfeI CAATTG 1 cut(s) 600
MflI RGATCY 5 cut(s) 167, 309, 701, 790, 967
MhlI GDGCHC 1 cut(s) 354
MlyI GAGTC 3 cut(s) 132, 1173, 1644
MmeI TCCRAC 1 cut(s) 1467
MnlI CCTC 5 cut(s) 577, 865, 1293, 1692, 1708
Mph1103I ATGCAT 1 cut(s) 1048
MslI CAYNNNNRTG 2 cut(s) 1251, 1587
MspCI CTTAAG 1 cut(s) 1358
MspR9I CCNGG 4 cut(s) 143, 153, 456, 1218
MunI CAATTG 1 cut(s) 600
MvaI CCWGG 4 cut(s) 143, 153, 456, 1218
MwoI GCNNNNNNNGC 4 cut(s) 615, 925, 1660, 1788
NdeII GATC 7 cut(s) 167, 309, 701, 790, 967, 1384, 1489
NlaIII CATG 6 cut(s) 1077, 1102, 1144, 1171, 1346, 1408
NmuCI GTSAC 3 cut(s) 218, 862, 1463
NsiI ATGCAT 1 cut(s) 1048
NspV TTCGAA 2 cut(s) 186, 1204
PagI TCATGA 1 cut(s) 1098
PaqCI CACCTGC 1 cut(s) 314
PceI AGGCCT 1 cut(s) 111
PcsI WCGNNNNNNNCGW 1 cut(s) 1360
PfoI TCCNGGA 2 cut(s) 141, 1216
PkrI GCNGC 1 cut(s) 1281
PleI GAGTC 3 cut(s) 132, 1173, 1643
PpsI GAGTC 3 cut(s) 132, 1173, 1643
PshBI ATTAAT 1 cut(s) 488
Psp124BI GAGCTC 1 cut(s) 354
Psp1406I AACGTT 1 cut(s) 182
Psp6I CCWGG 4 cut(s) 141, 151, 454, 1216
PspEI GGTNACC 1 cut(s) 862
PspGI CCWGG 4 cut(s) 141, 151, 454, 1216
PspPI GGNCC 1 cut(s) 97
PsuI RGATCY 5 cut(s) 167, 309, 701, 790, 967
RsaI GTAC 4 cut(s) 935, 1021, 1338, 1537
RsaNI GTAC 4 cut(s) 934, 1020, 1337, 1536
RseI CAYNNNNRTG 2 cut(s) 1251, 1587
SacI GAGCTC 1 cut(s) 354
SatI GCNGC 1 cut(s) 1280
Sau3AI GATC 7 cut(s) 167, 309, 701, 790, 967, 1384, 1489
Sau96I GGNCC 1 cut(s) 97
ScaI AGTACT 1 cut(s) 935
SchI GAGTC 3 cut(s) 132, 1173, 1644
ScrFI CCNGG 4 cut(s) 143, 153, 456, 1218
SduI GDGCHC 1 cut(s) 354
SfaNI GCATC 3 cut(s) 1033, 1055, 1121
SfcI CTRYAG 1 cut(s) 1399
SfuI TTCGAA 2 cut(s) 186, 1204
SinI GGWCC 1 cut(s) 97
SmiMI CAYNNNNRTG 2 cut(s) 1251, 1587
SmlI CTYRAG 5 cut(s) 133, 338, 443, 929, 1358
SmoI CTYRAG 5 cut(s) 133, 338, 443, 929, 1358
SpeI ACTAGT 1 cut(s) 1757
SseBI AGGCCT 1 cut(s) 111
SspI AATATT 2 cut(s) 229, 1228
SspMI CTAG 4 cut(s) 771, 998, 1185, 1758
SstI GAGCTC 1 cut(s) 354
StuI AGGCCT 1 cut(s) 111
StyD4I CCNGG 4 cut(s) 141, 151, 454, 1216
StyI CCWWGG 2 cut(s) 391, 770
TaaI ACNGT 1 cut(s) 938
TaiI ACGT 2 cut(s) 185, 1662
TaqI TCGA 4 cut(s) 186, 354, 1204, 1249
TatI WGTACW 4 cut(s) 933, 1019, 1336, 1535
TscAI CASTG 2 cut(s) 1032, 1771
TseFI GTSAC 3 cut(s) 218, 862, 1463
TseI GCWGC 1 cut(s) 1279
Tsp45I GTSAC 3 cut(s) 218, 862, 1463
TspGWI ACGGA 3 cut(s) 150, 1613, 1628
TspRI CASTG 2 cut(s) 1032, 1771
Vha464I CTTAAG 1 cut(s) 1358
VpaK11BI GGWCC 1 cut(s) 97
VspI ATTAAT 1 cut(s) 488
XapI RAATTY 5 cut(s) 58, 256, 839, 1051, 1424
XcmI CCANNNNNNNNNTGG 2 cut(s) 389, 1253
XmaJI CCTAGG 1 cut(s) 770
XspI CTAG 4 cut(s) 771, 998, 1185, 1758
ZrmI AGTACT 1 cut(s) 935
Zsp2I ATGCAT 1 cut(s) 1048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.