Rroxscaffold_4G00326450

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Reverse (-)
58568627 .. 58573227
4601 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326450.1

Sequence Viewer

Length: 1839 bp
ATGGCGTCAACCTCCTCTCGTCGTCAATGGAAATATGATGTGTTTCTAAGTTTCAGGGGACGAGATACCCGCAGTGTTTTTATCGATCATCTGTATTCTGCACTAACTCGGATAGGAATTCTCGCCTTTCGGGATGACCCAGAGCTTGAGAAAGGAAAATCCATCAGGCCTGAACTTTTAGCTGCAATTGAGGACTCCAGATGTGCAGTTGTCATTCTCTCACAGGACTATACTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATGCAATGCATGAAAGAGATGGGCCAACAGGTCCTCCCCGTCTTCTTCTGGGTGGATCCTTCAGATGTGCGGCACCAAAGGGGAAGTTTTGAGCTCAAATGGGAACCCAAAGTAGAAGTAAGGGAACATGAAGAAGTTTATGGGCAGAATGAGGACAGACTAAATGCATGGAGAGCTGCTTTGACTGAGGTGGCCAATCTTTCTGGCTGGCATTTCGAGTCTCATCAATATCTTACAGAACTAATTGACGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCGTTGATGATTTAATAGGCAATTACATATGTCCACAGCTTTGTGGGGGAACAGATTCAATCCAAGGCATGGTAATGGAGATGACAAAATTACAAGTGGCTCATTGGAATCCAGAAGCCTTTTCAAATATGTCTCAACTTAGTCTTCTCCATATTCATAATGTGGACCTTCCCAAAGGCCTCACTTGTCTTTCTAATTCCTTGACACTTCTCGAATGGACTGGGTATCCCTTAAGATCCCTCCCACAAAATTTTGAAGCAGATGAACTTATTGAACTTAACTTGTGCCACAGTAACATTGAACAGCTTTGGAAGGGAACAAAGTTCTTGCCTTCTGGATTGGTGCCAAGAGTGAATACAGAGCCAATGAGTTTCTGCTTGCCTATATCTGGCCTGTGCATTGGATTGCTTTCTAAGCTTGAGAACTTGAACTTGGAGAATTGCAAGACACTTCAAGAGTTGTCAGACCTTCCATCAAATAACATACTAGATTTAAGGGCAGATGGTTGTACTTCGCTGAAATACTTTTCTGAGGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATTTAAATGGCAATGAAGGATGCAATAACATAGCCTTAGAAATGCTGAAGACATTCTTGAATCAGGGAATCTCTAATAACAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCAAAGTGGTTCAGCCATTGGAGTGTTGAGTGTTCATTAAATTTTTTTCTACCTGAACATTGGAATAACAGTCGGTTTTTGGGATTTGCCTTGTGTGCTGTTTTTGTACTCCATGAGCACCATCGGGTGGATGAGCTTTATATAGATGCATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCAACGGAAGAGAATTGGAAGTCTATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTGAGTCAGACCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAGCAGTTGCAGTGAGGTTGAATTCTTATTTGAAACCAGAGGGATGGGTCTGGAGGTGAAGGAGTGTGGAGTTCGTTTTATATACGAGCAAGATGTACAAGGGTTAAACCAAACAACCACTCAATCAAGCAGTGGGATATCTCAAGAGGATATAGGGATGTCTCAAGAGGTTTTTATTCCGCTTGAAGAATTGTATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

612

Amino Acids

70.36

Weight (kDa)

5.1

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 189 2.1e-50 TIR domain
TIR_2 PF13676 14 - 107 5.3e-14 TIR domain
LRR_3 PF07725 289 - 307 1.5e-06 Leucine Rich Repeat
C-JID PF20160 430 - 579 2.2e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 342, 940
AccB7I CCANNNNNTGG 2 cut(s) 667, 1432
AccI GTMKAC 1 cut(s) 554
AccII CGCG 1 cut(s) 600
AciI CCGC 4 cut(s) 70, 340, 598, 1817
AclWI GGATC 3 cut(s) 320, 333, 828
AcoI YGGCCR 1 cut(s) 462
AcsI RAATTY 6 cut(s) 117, 847, 1138, 1345, 1559, 1658
AcuI CTGAAG 3 cut(s) 315, 1253, 1517
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 1108, 1413, 1624, 1734
AfiI CCNNNNNNNGG 3 cut(s) 667, 986, 1432
AflII CTTAAG 1 cut(s) 829
AjnI CCWGG 2 cut(s) 1291, 1566
AjuI GAANNNNNNNTTGG 2 cut(s) 1013, 1045
AluBI AGCT 9 cut(s) 145, 182, 364, 446, 637, 904, 1015, 1441, 1501
AluI AGCT 9 cut(s) 145, 182, 364, 446, 637, 904, 1015, 1441, 1501
Alw21I GWGCWC 2 cut(s) 366, 1425
Alw26I GTCTC 3 cut(s) 495, 735, 1803
AlwI GGATC 3 cut(s) 320, 333, 828
AoxI GGCC 5 cut(s) 167, 292, 462, 775, 988
ApeKI GCWGC 2 cut(s) 182, 446
ApoI RAATTY 6 cut(s) 117, 847, 1138, 1345, 1559, 1658
Asp700I GAANNNNTTC 4 cut(s) 592, 652, 1238, 1275
AspS9I GGNCC 3 cut(s) 292, 301, 763
AsuHPI GGTGA 1 cut(s) 1705
AvaII GGWCC 2 cut(s) 301, 763
BalI TGGCCA 1 cut(s) 464
BamHI GGATCC 1 cut(s) 325
BanI GGYRCC 2 cut(s) 342, 940
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 1605
BbsI GAAGAC 3 cut(s) 304, 734, 1241
Bbv12I GWGCWC 2 cut(s) 366, 1425
BbvI GCAGC 2 cut(s) 169, 433
BccI CCATC 6 cut(s) 170, 283, 1078, 1094, 1434, 1675
BciT130I CCWGG 2 cut(s) 1293, 1568
BciVI GTATCC 1 cut(s) 834
BcoDI GTCTC 3 cut(s) 495, 735, 1803
BfaI CTAG 2 cut(s) 1085, 1837
BfrI CTTAAG 1 cut(s) 829
BfuI GTATCC 1 cut(s) 834
BisI GCNGC 3 cut(s) 183, 341, 447
BlsI GCNGC 3 cut(s) 184, 342, 448
Bme1390I CCNGG 2 cut(s) 1293, 1568
Bme18I GGWCC 2 cut(s) 301, 763
BmgT120I GGNCC 3 cut(s) 292, 301, 763
BmiI GGNNCC 4 cut(s) 327, 344, 375, 942
BmrFI CCNGG 2 cut(s) 1293, 1568
BmrI ACTGGG 1 cut(s) 828
BmsI GCATC 3 cut(s) 1142, 1196, 1441
BmuI ACTGGG 1 cut(s) 828
BpiI GAAGAC 3 cut(s) 304, 734, 1241
BpmI CTGGAG 2 cut(s) 181, 1709
BpuEI CTTGAG 4 cut(s) 167, 1037, 1764, 1785
Bsa29I ATCGAT 1 cut(s) 84
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 661
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 3 cut(s) 667, 986, 1432
Bse1I ACTGG 1 cut(s) 823
Bse3DI GCAATG 2 cut(s) 281, 1204
BseBI CCWGG 2 cut(s) 1293, 1568
BseCI ATCGAT 1 cut(s) 84
BseDI CCNNGG 1 cut(s) 661
BseGI GGATG 6 cut(s) 139, 259, 1211, 1441, 1686, 1800
BseLI CCNNNNNNNGG 3 cut(s) 667, 986, 1432
BseMI GCAATG 2 cut(s) 281, 1204
BseMII CTCAG 2 cut(s) 447, 1119
BseNI ACTGG 1 cut(s) 823
BseRI GAGGAG 1 cut(s) 4
BseXI GCAGC 2 cut(s) 169, 433
BsgI GTGCAG 2 cut(s) 84, 225
Bsh1236I CGCG 1 cut(s) 600
BshFI GGCC 5 cut(s) 169, 294, 464, 777, 990
BshNI GGYRCC 2 cut(s) 342, 940
BshVI ATCGAT 1 cut(s) 84
BsiHKAI GWGCWC 2 cut(s) 366, 1425
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 3 cut(s) 667, 986, 1432
BsmAI GTCTC 3 cut(s) 495, 735, 1803
BsmFI GGGAC 1 cut(s) 72
BsmI GAATGC 1 cut(s) 1454
BsnI GGCC 5 cut(s) 169, 294, 464, 777, 990
Bsp1286I GDGCHC 2 cut(s) 366, 1425
Bsp1407I TGTACA 1 cut(s) 1732
Bsp143I GATC 3 cut(s) 85, 325, 833
BspACI CCGC 4 cut(s) 70, 340, 598, 1817
BspANI GGCC 5 cut(s) 169, 294, 464, 777, 990
BspCNI CTCAG 2 cut(s) 448, 1120
BspDI ATCGAT 1 cut(s) 84
BspFNI CGCG 1 cut(s) 600
BspLI GGNNCC 4 cut(s) 327, 344, 375, 942
BspPI GGATC 3 cut(s) 320, 333, 828
BspT107I GGYRCC 2 cut(s) 342, 940
BspTI CTTAAG 1 cut(s) 829
BsrDI GCAATG 2 cut(s) 281, 1204
BsrGI TGTACA 1 cut(s) 1732
BsrI ACTGG 1 cut(s) 823
BssECI CCNNGG 1 cut(s) 661
BssMI GATC 3 cut(s) 85, 325, 833
BssNAI GTATAC 1 cut(s) 555
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1605
BssT1I CCWWGG 1 cut(s) 661
Bst1107I GTATAC 1 cut(s) 555
Bst2BI CACGAG 1 cut(s) 1605
Bst2UI CCWGG 2 cut(s) 1293, 1568
Bst4CI ACNGT 2 cut(s) 890, 1376
Bst6I CTCTTC 1 cut(s) 1504
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 829
BstAUI TGTACA 1 cut(s) 1732
BstC8I GCNNGC 3 cut(s) 479, 977, 1539
BstDEI CTNAG 6 cut(s) 47, 456, 737, 1011, 1128, 1222
BstF5I GGATG 6 cut(s) 139, 259, 1211, 1441, 1686, 1800
BstFNI CGCG 1 cut(s) 600
BstKTI GATC 3 cut(s) 88, 328, 836
BstMAI GTCTC 3 cut(s) 495, 735, 1803
BstMBI GATC 3 cut(s) 85, 325, 833
BstMWI GCNNNNNNNGC 3 cut(s) 443, 1012, 1400
BstNI CCWGG 2 cut(s) 1293, 1568
BstSCI CCNGG 2 cut(s) 1291, 1566
BstUI CGCG 1 cut(s) 600
BstV1I GCAGC 2 cut(s) 169, 433
BstV2I GAAGAC 3 cut(s) 304, 734, 1241
BstX2I RGATCY 2 cut(s) 325, 833
BstXI CCANNNNNNTGG 1 cut(s) 1681
BstYI RGATCY 2 cut(s) 325, 833
BstZ17I GTATAC 1 cut(s) 555
Bsu15I ATCGAT 1 cut(s) 84
BsuI GTATCC 1 cut(s) 834
BsuRI GGCC 5 cut(s) 169, 294, 464, 777, 990
BsuTUI ATCGAT 1 cut(s) 84
BtsCI GGATG 6 cut(s) 139, 259, 1211, 1441, 1686, 1800
BtsI GCAGTG 3 cut(s) 79, 1654, 1774
BtsIMutI CAGTG 3 cut(s) 79, 1654, 1774
Cac8I GCNNGC 3 cut(s) 479, 977, 1539
Cfr13I GGNCC 3 cut(s) 292, 301, 763
ClaI ATCGAT 1 cut(s) 84
Csp6I GTAC 4 cut(s) 1107, 1412, 1623, 1733
CviAII CATG 6 cut(s) 243, 280, 398, 438, 667, 1418
CviQI GTAC 4 cut(s) 1107, 1412, 1623, 1733
DdeI CTNAG 6 cut(s) 47, 456, 737, 1011, 1128, 1222
DpnI GATC 3 cut(s) 87, 327, 835
DpnII GATC 3 cut(s) 85, 325, 833
DraI TTTAAA 1 cut(s) 1191
EaeI YGGCCR 1 cut(s) 462
Eam1104I CTCTTC 1 cut(s) 1504
EarI CTCTTC 1 cut(s) 1504
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 661
Eco147I AGGCCT 2 cut(s) 169, 777
Eco24I GRGCYC 1 cut(s) 366
Eco32I GATATC 1 cut(s) 1776
Eco47I GGWCC 2 cut(s) 301, 763
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 3 cut(s) 315, 1253, 1517
EcoICRI GAGCTC 1 cut(s) 364
EcoO109I RGGNCCY 1 cut(s) 301
EcoRI GAATTC 2 cut(s) 117, 1658
EcoRII CCWGG 2 cut(s) 1291, 1566
EcoRV GATATC 1 cut(s) 1776
EcoT14I CCWWGG 1 cut(s) 661
EcoT22I ATGCAT 3 cut(s) 281, 439, 1456
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 661
FaeI CATG 6 cut(s) 246, 283, 401, 441, 670, 1421
FalI AAGNNNNNCTT 2 cut(s) 1226, 1258
FaqI GGGAC 1 cut(s) 72
FatI CATG 6 cut(s) 242, 279, 397, 437, 666, 1417
FauI CCCGC 2 cut(s) 77, 605
FauNDI CATATG 1 cut(s) 626
FblI GTMKAC 1 cut(s) 554
Fnu4HI GCNGC 3 cut(s) 183, 341, 447
FokI GGATG 6 cut(s) 146, 266, 1218, 1448, 1693, 1807
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 3 cut(s) 183, 341, 447
FspBI CTAG 2 cut(s) 1085, 1837
GluI GCNGC 3 cut(s) 183, 341, 447
GsuI CTGGAG 2 cut(s) 181, 1709
HaeIII GGCC 5 cut(s) 169, 294, 464, 777, 990
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 246, 283, 401, 441, 670, 1421
HincII GTYRAC 2 cut(s) 9, 571
HindII GTYRAC 2 cut(s) 9, 571
HindIII AAGCTT 1 cut(s) 1013
HinfI GANTC 8 cut(s) 194, 488, 593, 653, 706, 1246, 1254, 1574
HphI GGTGA 1 cut(s) 1705
Hpy166II GTNNAC 5 cut(s) 9, 555, 571, 632, 763
Hpy188I TCNGA 5 cut(s) 111, 334, 1063, 1129, 1579
Hpy8I GTNNAC 5 cut(s) 9, 555, 571, 632, 763
Hpy99I CGWCG 1 cut(s) 24
HpyAV CCTTC 7 cut(s) 339, 776, 904, 939, 1076, 1196, 1690
HpyCH4III ACNGT 2 cut(s) 890, 1376
HpyF10VI GCNNNNNNNGC 3 cut(s) 443, 1012, 1400
HpyF3I CTNAG 6 cut(s) 47, 456, 737, 1011, 1128, 1222
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 246, 283, 401, 441, 670, 1421
Kzo9I GATC 3 cut(s) 85, 325, 833
Lsp1109I GCAGC 2 cut(s) 169, 433
LweI GCATC 3 cut(s) 1142, 1196, 1441
MaeI CTAG 2 cut(s) 1085, 1837
MaeIII GTNAC 1 cut(s) 890
MalI GATC 3 cut(s) 87, 327, 835
MboI GATC 3 cut(s) 85, 325, 833
MfeI CAATTG 2 cut(s) 186, 1177
MflI RGATCY 2 cut(s) 325, 833
MhlI GDGCHC 2 cut(s) 366, 1425
MlsI TGGCCA 1 cut(s) 464
MluNI TGGCCA 1 cut(s) 464
MlyI GAGTC 3 cut(s) 188, 497, 1583
Mox20I TGGCCA 1 cut(s) 464
Mph1103I ATGCAT 3 cut(s) 281, 439, 1456
MroXI GAANNNNTTC 4 cut(s) 592, 652, 1238, 1275
MscI TGGCCA 1 cut(s) 464
MslI CAYNNNNRTG 3 cut(s) 587, 671, 1326
Msp20I TGGCCA 1 cut(s) 464
MspCI CTTAAG 1 cut(s) 829
MspR9I CCNGG 2 cut(s) 1293, 1568
MunI CAATTG 2 cut(s) 186, 1177
Mva1269I GAATGC 1 cut(s) 1454
MvaI CCWGG 2 cut(s) 1293, 1568
MvnI CGCG 1 cut(s) 600
MwoI GCNNNNNNNGC 3 cut(s) 443, 1012, 1400
NdeI CATATG 1 cut(s) 626
NdeII GATC 3 cut(s) 85, 325, 833
NlaIII CATG 6 cut(s) 246, 283, 401, 441, 670, 1421
NlaIV GGNNCC 4 cut(s) 327, 344, 375, 942
NsiI ATGCAT 3 cut(s) 281, 439, 1456
PceI AGGCCT 2 cut(s) 169, 777
PctI GAATGC 1 cut(s) 1454
PdmI GAANNNNTTC 4 cut(s) 592, 652, 1238, 1275
PfeI GAWTC 5 cut(s) 593, 653, 706, 1246, 1254
PflMI CCANNNNNTGG 2 cut(s) 667, 1432
PfoI TCCNGGA 1 cut(s) 1291
PkrI GCNGC 3 cut(s) 184, 342, 448
PleI GAGTC 3 cut(s) 188, 496, 1582
PpsI GAGTC 3 cut(s) 188, 496, 1582
PpuMI RGGWCCY 1 cut(s) 301
Psp124BI GAGCTC 1 cut(s) 366
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 2 cut(s) 1291, 1566
PspGI CCWGG 2 cut(s) 1291, 1566
PspN4I GGNNCC 4 cut(s) 327, 344, 375, 942
PspPI GGNCC 3 cut(s) 292, 301, 763
PspPPI RGGWCCY 1 cut(s) 301
PsuI RGATCY 2 cut(s) 325, 833
RsaI GTAC 4 cut(s) 1108, 1413, 1624, 1734
RsaNI GTAC 4 cut(s) 1107, 1412, 1623, 1733
RseI CAYNNNNRTG 3 cut(s) 587, 671, 1326
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 3 cut(s) 183, 341, 447
Sau3AI GATC 3 cut(s) 85, 325, 833
Sau96I GGNCC 3 cut(s) 292, 301, 763
SchI GAGTC 3 cut(s) 188, 497, 1583
ScrFI CCNGG 2 cut(s) 1293, 1568
SduI GDGCHC 2 cut(s) 366, 1425
SfaNI GCATC 3 cut(s) 1142, 1196, 1441
SinI GGWCC 2 cut(s) 301, 763
SmiI ATTTAAAT 1 cut(s) 1191
SmiMI CAYNNNNRTG 3 cut(s) 587, 671, 1326
SmlI CTYRAG 5 cut(s) 146, 829, 1016, 1779, 1800
SmoI CTYRAG 5 cut(s) 146, 829, 1016, 1779, 1800
SseBI AGGCCT 2 cut(s) 169, 777
SsiI CCGC 4 cut(s) 70, 340, 598, 1817
SspI AATATT 1 cut(s) 1303
SspMI CTAG 2 cut(s) 1085, 1837
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 2 cut(s) 169, 777
StyD4I CCNGG 2 cut(s) 1291, 1566
StyI CCWWGG 1 cut(s) 661
SwaI ATTTAAAT 1 cut(s) 1191
TaaI ACNGT 2 cut(s) 890, 1376
TaqI TCGA 3 cut(s) 84, 486, 810
TatI WGTACW 3 cut(s) 1106, 1411, 1732
TauI GCSGC 1 cut(s) 343
TfiI GAWTC 5 cut(s) 593, 653, 706, 1246, 1254
TscAI CASTG 3 cut(s) 79, 1654, 1774
TseI GCWGC 2 cut(s) 182, 446
TspDTI ATGAA 9 cut(s) 270, 296, 414, 571, 743, 876, 1162, 1215, 1329
TspGWI ACGGA 2 cut(s) 538, 1521
TspRI CASTG 3 cut(s) 79, 1654, 1774
Van91I CCANNNNNTGG 2 cut(s) 667, 1432
Vha464I CTTAAG 1 cut(s) 829
VpaK11BI GGWCC 2 cut(s) 301, 763
XapI RAATTY 6 cut(s) 117, 847, 1138, 1345, 1559, 1658
XmiI GTMKAC 1 cut(s) 554
XmnI GAANNNNTTC 4 cut(s) 592, 652, 1238, 1275
XspI CTAG 2 cut(s) 1085, 1837
Zsp2I ATGCAT 3 cut(s) 281, 439, 1456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.