RchiOBHm_Chr6g0250681

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000105
Physical Location & Seq
Forward (+)
6069619 .. 6073850
4232 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22471

Sequence Viewer

Length: 2856 bp
ATGGATTCCCGTATTGATGATTTATTAAGCAATTACATATGTCCGCAGCTTGGTCGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATGAAATTTGTCAAGATTTTGACAGAAGCTGCATTCTTTACAATGTTAGAGAAAAATCTGAAAAGGATGGTCTAGTTTCTCTACAAGAAAAACTACTTTCCAGAATCCTGAGGACAAAAGTTGACATTGAGGATGAATATGCAGGAGCTACTATGATAGAAAGGCGGTTATGTAAACTAAAGGTGCTTGTTGTTATTGATGATGTGGATCACATCAATCAATTAGATAAATTGGCTGGAAGTCGGGACTGGTTCGGTCCGGGGAGTAGAATTATTGTAACCACTCCAGATATTCATTTGCTAAGGGAACATGATGTTGATGCTACATACAAGGCTACTGGGTTAACTCGTGGTGAAGCTATTCAACTATTGAGTTTAAAGGCTTTCAAGAAAAGCTTCCCACCAGAAGATTATTTGGACTTGTGCCACCATATTATACGGTATGCTCAGGGGCTTCCGTTGGCTCTTGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGACCCAATGAATGGGAAAGTACAATAGAGAGGCTAAACAATACACCAAATAGACAAGTTATGGATGTGCTTCAGATTAGTTTTGATGGACTAGAGGAAAAAGACAAACAGATATTCCTACATATTGCTTGTTTGTACAAGGGGAAGGACAGGGATCGTGTGACACAAATACTAGGCTATTGCAAGCTAGAACCTGGCATTGGTTTAAGGGTTCTTGAGGAAAAATCTCTCATTACTATTTTTAACAACAAACTGTCAATGCATGATTTGCTACAAGAAATGGGCTTGGAAATAGTTCGTCGACAGTCCCCTGAAGAGCCAGGTAAACGAAGTAGATTATGGTCTCCTGAAGATATCCACAATGTGCTGAAGAAAAATAAGGGAACAGACAAAATCCGAGCCATGGTAATGGACTTGCCTGAGTTAGAAGTGGCTCATTGGAAACCAGAAGCCTTTTCAAATTTGTCTCAACTCAGTCTTCTCCATATTCGTAACGTGGATCTTCCCGAAGGCCTCACTTTTCTATCTAATTCCTTGAGACTCCTTGATTGGAGTGGGTACCCCTTAAGATCCCTCCCACAAAATTTTGAACCAGACCAACTTATTGAACTTAACTTGTGCCACAGCAACATTGAACACCTCTGGAAAGGAGCAAAGAATTTTGACAAGTTAAAGTTCATCAAACTATGCCATTCTCAAAAAATTGTCCAGACCCCAGACCTCACAGGCGTTCAAAATCTTGAGACTTTAGATCTTGAAGGATGTAAGACTTTGGTAAAACTTCATCAATCCCTCGGACAACTCAAAAGGCTTATTGTATTGAATCTTAAAGATTGCGAGAATCTCGAGAACCTGCCAAGTATGATTGAAATGGAATCTCTTGAAACTTTAATTCTTTCTAACTGCTCAAAAGTTAAAAAGATTCCTGAGTTCGCTGGAAATATGAAGCGTTTATCAAAGCTTTATCTCGGTGAGACTGCCATTGAAAAACTACCTGTGTCAATTGGATGCCTGAGTGGCCTTGCTTCACTGAATCTTAGCAACTGCAAAAATCTTGTTTGCCTTCCAAGCACCTTTAGTATATTGGAGTCTCTTGAAAATGTTAATCTTTCTGGATGTTTGAAACTTGGGGAACAGGATAGTGCAAGGGAGAATGGTGCAATTAGAACTGCTGTAAACCATCTTATAAAACGTGGTTGTGAAGATGTTGGGAAATATCTGTCATGGTCCTTGCCTTCTAGATTGGTGCAAAGAGCCGATATAGAGCCAATAAGTATGAAGTTGCCTGTATCTGATCTGTGTTATTCTACAGAGCAAAATGCAAATACATCAATGAGTATCCGGTTGCCACTATCTCATCTATGTAATTTAACAGATCTAAACTTAAGTAACTGCAATCTTAGTGACACTGCATTTGCCAACTTTGCCTGTTTTCCCTCCTTAGTGGCACTAAATCTGAGTGGAAATAATTTTGTTAAGCTTCCTCCAAGCATCAGGTCCTGTTTTAAGCTTCAGAACATTAATTTGGAGAATTGCAAGACACTTCAAGAATTATCAGGACTTCCATCAAATAGTAAATTGGATGTGAGGGCAGATGGTTGTTCTTCACTGGAAATGCTGTTTGATGTATCCAATTTCAATAGATTGGAGAAATTATATTTCAATTTCATCAATTGCTTCAAACTAAATGACAATCAGGGCTGCAGTAACATAGCGTTTGAAATGCTGAGGACATTCAGTAATCAGCGAATTTCTAGTGCCACGGAAACTTTTCAAATTGTAATTCCTGGAAGCAGAATTCCTGAGTGGTTTGCTTGTCGAATATTTGGGAGTTCATTAATTGTAGGCCTAGTTCCTGGTTGGAATAAAAGTAGGATTTTGGGATTTGTTTTGTGTGCTGTTTTTGTACTCCATGAGCACCGTCACGTTGATGAGCTTGATATCTGTCAGTTCAAGACTTTTAAAGCTACTCATCATCTTGTATGTTGCCTGAACCTCAATGGAAGAGAATTAGAAGTATACGGCAAACAGCCTGCATTTCGCTTTAGCGAACAATTTTGCCAGGTTGAGTCAGATCACCTTTGGATATTCTATGTGTCCCGTGATAAATACTGTGGTCCTGATTGGTGCCATAACAGTTGCAGTCAGCTTGATTTCTTATTTGAAACAAGAGGGCCTGGTCTGGAGGTGAAGGAGTGTGGGCTACGTCTGATATACGAGCAAGATGTGATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

951

Amino Acids

107.93

Weight (kDa)

6.6

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 20 - 173 9.1e-17 NB-ARC domain
AAA_22 PF13401 21 - 119 5e-07 AAA domain
NACHT PF05729 23 - 119 5.6e-06 NACHT domain
WHD_ROQ1 PF23282 239 - 310 1.5e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 499 - 585 2.2e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 664 - 751 5.5e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 802 - 950 1.6e-11 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1808
Acc36I ACCTGC 1 cut(s) 1482
Acc65I GGTACC 1 cut(s) 1179
AccB1I GGYRCC 2 cut(s) 1179, 2748
AccB7I CCANNNNNTGG 1 cut(s) 633
AccI GTMKAC 2 cut(s) 922, 2640
AciI CCGC 3 cut(s) 44, 85, 289
AclWI GGATC 6 cut(s) 67, 80, 339, 783, 1128, 1185
AcsI RAATTY 6 cut(s) 129, 1081, 1204, 1279, 2370, 2418
AcuI CTGAAG 5 cut(s) 677, 954, 990, 1010, 2117
AdeI CACNNNGTG 1 cut(s) 985
AfaI GTAC 4 cut(s) 643, 758, 1181, 2529
AfiI CCNNNNNNNGG 4 cut(s) 50, 633, 821, 1024
AflII CTTAAG 2 cut(s) 1186, 2005
AjnI CCWGG 6 cut(s) 814, 940, 2407, 2476, 2682, 2797
AjuI GAANNNNNNNTTGG 4 cut(s) 804, 836, 2129, 2161
Alw21I GWGCWC 1 cut(s) 2541
Alw26I GTCTC 6 cut(s) 969, 1092, 1153, 1358, 1589, 1716
AlwI GGATC 6 cut(s) 67, 80, 339, 783, 1128, 1185
AlwNI CAGNNNCTG 2 cut(s) 153, 2121
Ama87I CYCGRG 2 cut(s) 111, 1466
AoxI GGCC 4 cut(s) 1132, 1639, 2467, 2795
ApeKI GCWGC 3 cut(s) 46, 153, 2322
ApoI RAATTY 6 cut(s) 129, 1081, 1204, 1279, 2370, 2418
ArsI GACNNNNNNTTYG 2 cut(s) 2018, 2050
AseI ATTAAT 2 cut(s) 2142, 2459
Asp700I GAANNNNTTC 4 cut(s) 483, 518, 915, 2391
Asp718I GGTACC 1 cut(s) 1179
AspS9I GGNCC 5 cut(s) 380, 1848, 2118, 2738, 2795
AsuC2I CCSGG 1 cut(s) 384
AsuHPI GGTGA 4 cut(s) 488, 1604, 2690, 2821
AvaI CYCGRG 2 cut(s) 111, 1466
AvaII GGWCC 4 cut(s) 380, 1848, 2118, 2738
AxyI CCTNAGG 1 cut(s) 233
BamHI GGATCC 1 cut(s) 72
BanI GGYRCC 2 cut(s) 1179, 2748
BauI CACGAG 1 cut(s) 471
BbsI GAAGAC 2 cut(s) 628, 1091
Bbv12I GWGCWC 1 cut(s) 2541
BbvCI CCTCAGC 1 cut(s) 2348
BbvI GCAGC 3 cut(s) 58, 140, 2309
BccI CCATC 5 cut(s) 185, 701, 1809, 2194, 2210
BceAI ACGGC 1 cut(s) 2659
BcgI CGANNNNNNTGC 2 cut(s) 35, 69
BciT130I CCWGG 6 cut(s) 816, 942, 2409, 2478, 2684, 2799
BciVI GTATCC 2 cut(s) 1970, 2260
BcnI CCSGG 1 cut(s) 384
BcoDI GTCTC 6 cut(s) 969, 1092, 1153, 1358, 1589, 1716
BfaI CTAG 7 cut(s) 197, 713, 794, 809, 1860, 2376, 2471
BfmI CTRYAG 2 cut(s) 1929, 2323
BfrI CTTAAG 2 cut(s) 1186, 2005
BfuAI ACCTGC 1 cut(s) 1482
BfuI GTATCC 2 cut(s) 1970, 2260
BglI GCCNNNNNGGC 1 cut(s) 1638
BglII AGATCT 2 cut(s) 1372, 1996
BisI GCNGC 3 cut(s) 47, 154, 2323
BlsI GCNGC 3 cut(s) 48, 155, 2324
Bme1390I CCNGG 7 cut(s) 384, 816, 942, 2409, 2478, 2684, 2799
Bme18I GGWCC 4 cut(s) 380, 1848, 2118, 2738
BmeT110I CYCGRG 2 cut(s) 111, 1466
BmgT120I GGNCC 5 cut(s) 380, 1848, 2118, 2738, 2795
BmiI GGNNCC 3 cut(s) 74, 1181, 2750
BmrFI CCNGG 7 cut(s) 384, 816, 942, 2409, 2478, 2684, 2799
BmrI ACTGGG 1 cut(s) 471
BmsI GCATC 3 cut(s) 433, 1619, 2121
BmuI ACTGGG 1 cut(s) 471
BpiI GAAGAC 2 cut(s) 628, 1091
BpmI CTGGAG 2 cut(s) 393, 2825
Bpu10I CCTNAGC 3 cut(s) 425, 570, 2348
BpuEI CTTGAG 3 cut(s) 857, 1177, 1382
BpuMI CCSGG 1 cut(s) 384
BsaBI GATNNNNATC 1 cut(s) 330
BsaI GGTCTC 1 cut(s) 969
BsaJI CCNNGG 5 cut(s) 76, 383, 1023, 1414, 2382
BsaWI WCCGGW 1 cut(s) 1962
Bsc4I CCNNNNNNNGG 4 cut(s) 50, 633, 821, 1024
Bse1I ACTGG 3 cut(s) 377, 466, 2235
Bse21I CCTNAGG 1 cut(s) 233
Bse8I GATNNNNATC 1 cut(s) 330
BseBI CCWGG 6 cut(s) 816, 942, 2409, 2478, 2684, 2799
BseDI CCNNGG 5 cut(s) 76, 383, 1023, 1414, 2382
BseGI GGATG 7 cut(s) 196, 262, 691, 1388, 1634, 1742, 2209
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 4 cut(s) 50, 633, 821, 1024
BseMII CTCAG 9 cut(s) 224, 584, 1032, 1108, 1539, 1625, 2069, 2339, 2415
BseNI ACTGG 3 cut(s) 377, 466, 2235
BseXI GCAGC 3 cut(s) 58, 140, 2309
BshFI GGCC 4 cut(s) 1134, 1641, 2469, 2797
BshNI GGYRCC 2 cut(s) 1179, 2748
BsiHKAI GWGCWC 1 cut(s) 2541
BsiHKCI CYCGRG 2 cut(s) 111, 1466
BsiSI CCGG 2 cut(s) 383, 1963
BslFI GGGAC 3 cut(s) 383, 913, 2704
BslI CCNNNNNNNGG 4 cut(s) 50, 633, 821, 1024
BsmAI GTCTC 6 cut(s) 969, 1092, 1153, 1358, 1589, 1716
BsmFI GGGAC 3 cut(s) 383, 913, 2704
BsmI GAATGC 1 cut(s) 156
BsnI GGCC 4 cut(s) 1134, 1641, 2469, 2797
Bso31I GGTCTC 1 cut(s) 969
BsoBI CYCGRG 2 cut(s) 111, 1466
Bsp1286I GDGCHC 1 cut(s) 2541
Bsp1407I TGTACA 1 cut(s) 756
Bsp143I GATC 9 cut(s) 72, 331, 775, 1120, 1190, 1372, 1915, 1996, 2695
Bsp19I CCATGG 2 cut(s) 76, 1023
BspACI CCGC 3 cut(s) 44, 85, 289
BspANI GGCC 4 cut(s) 1134, 1641, 2469, 2797
BspCNI CTCAG 9 cut(s) 225, 583, 1033, 1107, 1540, 1626, 2070, 2340, 2416
BspLI GGNNCC 3 cut(s) 74, 1181, 2750
BspMAI CTGCAG 1 cut(s) 2327
BspMI ACCTGC 1 cut(s) 1482
BspPI GGATC 6 cut(s) 67, 80, 339, 783, 1128, 1185
BspQI GCTCTTC 1 cut(s) 930
BspT107I GGYRCC 2 cut(s) 1179, 2748
BspTI CTTAAG 2 cut(s) 1186, 2005
BspTNI GGTCTC 1 cut(s) 969
BsrGI TGTACA 1 cut(s) 756
BsrI ACTGG 3 cut(s) 377, 466, 2235
BssECI CCNNGG 5 cut(s) 76, 383, 1023, 1414, 2382
BssMI GATC 9 cut(s) 72, 331, 775, 1120, 1190, 1372, 1915, 1996, 2695
BssNAI GTATAC 1 cut(s) 2641
BssSI CACGAG 1 cut(s) 471
BssT1I CCWWGG 2 cut(s) 76, 1023
Bst1107I GTATAC 1 cut(s) 2641
Bst2BI CACGAG 1 cut(s) 471
Bst2UI CCWGG 6 cut(s) 816, 942, 2409, 2478, 2684, 2799
Bst4CI ACNGT 6 cut(s) 564, 876, 927, 2543, 2735, 2759
Bst6I CTCTTC 2 cut(s) 930, 2620
BstAFI CTTAAG 2 cut(s) 1186, 2005
BstAPI GCANNNNNTGC 1 cut(s) 889
BstAUI TGTACA 1 cut(s) 756
BstC8I GCNNGC 3 cut(s) 83, 806, 2655
BstDSI CCRYGG 3 cut(s) 76, 1023, 2382
BstF5I GGATG 7 cut(s) 196, 262, 691, 1388, 1634, 1742, 2209
BstKTI GATC 9 cut(s) 75, 334, 778, 1123, 1193, 1375, 1918, 1999, 2698
BstMAI GTCTC 6 cut(s) 969, 1092, 1153, 1358, 1589, 1716
BstMBI GATC 9 cut(s) 72, 331, 775, 1120, 1190, 1372, 1915, 1996, 2695
BstMWI GCNNNNNNNGC 5 cut(s) 87, 889, 1638, 1689, 2045
BstNI CCWGG 6 cut(s) 816, 942, 2409, 2478, 2684, 2799
BstNSI RCATGY 1 cut(s) 85
BstSCI CCNGG 7 cut(s) 382, 814, 940, 2407, 2476, 2682, 2797
BstSFI CTRYAG 2 cut(s) 1929, 2323
BstV1I GCAGC 3 cut(s) 58, 140, 2309
BstV2I GAAGAC 2 cut(s) 628, 1091
BstX2I RGATCY 5 cut(s) 72, 1120, 1190, 1372, 1996
BstXI CCANNNNNNTGG 1 cut(s) 1030
BstYI RGATCY 5 cut(s) 72, 1120, 1190, 1372, 1996
BstZ17I GTATAC 1 cut(s) 2641
Bsu36I CCTNAGG 1 cut(s) 233
BsuI GTATCC 2 cut(s) 1970, 2260
BsuRI GGCC 4 cut(s) 1134, 1641, 2469, 2797
BtgI CCRYGG 3 cut(s) 76, 1023, 2382
BtsCI GGATG 7 cut(s) 196, 262, 691, 1388, 1634, 1742, 2209
BtsI GCAGTG 1 cut(s) 2028
BtsIMutI CAGTG 3 cut(s) 1649, 2028, 2228
BveI ACCTGC 1 cut(s) 1482
Cac8I GCNNGC 3 cut(s) 83, 806, 2655
CaiI CAGNNNCTG 2 cut(s) 153, 2121
Cfr13I GGNCC 5 cut(s) 380, 1848, 2118, 2738, 2795
CpoI CGGWCCG 1 cut(s) 380
Csp6I GTAC 4 cut(s) 642, 757, 1180, 2528
CspI CGGWCCG 1 cut(s) 380
CviAII CATG 8 cut(s) 77, 82, 122, 434, 884, 1024, 1845, 2534
CviQI GTAC 4 cut(s) 642, 757, 1180, 2528
DpnI GATC 9 cut(s) 74, 333, 777, 1122, 1192, 1374, 1917, 1998, 2697
DpnII GATC 9 cut(s) 72, 331, 775, 1120, 1190, 1372, 1915, 1996, 2695
DraI TTTAAA 2 cut(s) 501, 2584
DraIII CACNNNGTG 1 cut(s) 985
Eam1104I CTCTTC 2 cut(s) 930, 2620
EarI CTCTTC 2 cut(s) 930, 2620
Eco130I CCWWGG 2 cut(s) 76, 1023
Eco147I AGGCCT 2 cut(s) 1134, 2469
Eco31I GGTCTC 1 cut(s) 969
Eco32I GATATC 2 cut(s) 976, 2563
Eco47I GGWCC 4 cut(s) 380, 1848, 2118, 2738
Eco57I CTGAAG 5 cut(s) 677, 954, 990, 1010, 2117
Eco81I CCTNAGG 1 cut(s) 233
Eco88I CYCGRG 2 cut(s) 111, 1466
EcoO109I RGGNCCY 2 cut(s) 2118, 2795
EcoRI GAATTC 1 cut(s) 2418
EcoRII CCWGG 6 cut(s) 814, 940, 2407, 2476, 2682, 2797
EcoRV GATATC 2 cut(s) 976, 2563
EcoT14I CCWWGG 2 cut(s) 76, 1023
EcoT22I ATGCAT 1 cut(s) 885
ErhI CCWWGG 2 cut(s) 76, 1023
FaeI CATG 8 cut(s) 80, 85, 125, 437, 887, 1027, 1848, 2537
FalI AAGNNNNNCTT 2 cut(s) 503, 535
FaqI GGGAC 3 cut(s) 383, 913, 2704
FatI CATG 8 cut(s) 76, 81, 121, 433, 883, 1023, 1844, 2533
FauI CCCGC 1 cut(s) 78
FauNDI CATATG 1 cut(s) 38
FblI GTMKAC 2 cut(s) 922, 2640
Fnu4HI GCNGC 3 cut(s) 47, 154, 2323
FokI GGATG 7 cut(s) 203, 269, 698, 1395, 1641, 1749, 2216
Fsp4HI GCNGC 3 cut(s) 47, 154, 2323
FspBI CTAG 7 cut(s) 197, 713, 794, 809, 1860, 2376, 2471
GluI GCNGC 3 cut(s) 47, 154, 2323
GsuI CTGGAG 2 cut(s) 393, 2825
HaeIII GGCC 4 cut(s) 1134, 1641, 2469, 2797
HapII CCGG 2 cut(s) 383, 1963
Hin1II CATG 8 cut(s) 80, 85, 125, 437, 887, 1027, 1848, 2537
HincII GTYRAC 3 cut(s) 247, 468, 923
HindII GTYRAC 3 cut(s) 247, 468, 923
HindIII AAGCTT 4 cut(s) 517, 1580, 2099, 2129
HpaI GTTAAC 1 cut(s) 468
HpaII CCGG 2 cut(s) 383, 1963
HphI GGTGA 4 cut(s) 488, 1604, 2690, 2821
Hpy166II GTNNAC 7 cut(s) 247, 299, 468, 923, 947, 1798, 2641
Hpy188I TCNGA 9 cut(s) 184, 696, 1019, 1418, 1915, 2079, 2136, 2695, 2832
Hpy8I GTNNAC 7 cut(s) 247, 299, 468, 923, 947, 1798, 2641
Hpy99I CGWCG 1 cut(s) 924
HpyAV CCTTC 6 cut(s) 760, 1124, 1373, 1694, 1866, 2806
HpyCH4III ACNGT 6 cut(s) 564, 876, 927, 2543, 2735, 2759
HpyCH4IV ACGT 4 cut(s) 1116, 1813, 2547, 2827
HpyF10VI GCNNNNNNNGC 5 cut(s) 87, 889, 1638, 1689, 2045
HpySE526I ACGT 4 cut(s) 1116, 1813, 2547, 2827
Hsp92II CATG 8 cut(s) 80, 85, 125, 437, 887, 1027, 1848, 2537
KpnI GGTACC 1 cut(s) 1183
KspAI GTTAAC 1 cut(s) 468
Kzo9I GATC 9 cut(s) 72, 331, 775, 1120, 1190, 1372, 1915, 1996, 2695
LguI GCTCTTC 1 cut(s) 930
LmnI GCTCC 2 cut(s) 269, 1271
Lsp1109I GCAGC 3 cut(s) 58, 140, 2309
LweI GCATC 3 cut(s) 433, 1619, 2121
MaeI CTAG 7 cut(s) 197, 713, 794, 809, 1860, 2376, 2471
MaeII ACGT 4 cut(s) 1116, 1813, 2547, 2827
MaeIII GTNAC 7 cut(s) 400, 781, 1112, 2009, 2024, 2327, 2543
MalI GATC 9 cut(s) 74, 333, 777, 1122, 1192, 1374, 1917, 1998, 2697
MboI GATC 9 cut(s) 72, 331, 775, 1120, 1190, 1372, 1915, 1996, 2695
MfeI CAATTG 2 cut(s) 1623, 2293
MflI RGATCY 5 cut(s) 72, 1120, 1190, 1372, 1996
MhlI GDGCHC 1 cut(s) 2541
MlyI GAGTC 3 cut(s) 1155, 1718, 2699
MmeI TCCRAC 1 cut(s) 2462
Mph1103I ATGCAT 1 cut(s) 885
MroXI GAANNNNTTC 4 cut(s) 483, 518, 915, 2391
MslI CAYNNNNRTG 2 cut(s) 1028, 2550
MspCI CTTAAG 2 cut(s) 1186, 2005
MspI CCGG 2 cut(s) 383, 1963
MspR9I CCNGG 7 cut(s) 384, 816, 942, 2409, 2478, 2684, 2799
MunI CAATTG 2 cut(s) 1623, 2293
Mva1269I GAATGC 1 cut(s) 156
MvaI CCWGG 6 cut(s) 816, 942, 2409, 2478, 2684, 2799
MwoI GCNNNNNNNGC 5 cut(s) 87, 889, 1638, 1689, 2045
NciI CCSGG 1 cut(s) 384
NcoI CCATGG 2 cut(s) 76, 1023
NdeI CATATG 1 cut(s) 38
NdeII GATC 9 cut(s) 72, 331, 775, 1120, 1190, 1372, 1915, 1996, 2695
NlaIII CATG 8 cut(s) 80, 85, 125, 437, 887, 1027, 1848, 2537
NlaIV GGNNCC 3 cut(s) 74, 1181, 2750
NmuCI GTSAC 3 cut(s) 781, 2024, 2543
NsiI ATGCAT 1 cut(s) 885
NspI RCATGY 1 cut(s) 85
PaeI GCATGC 1 cut(s) 85
PaeR7I CTCGAG 2 cut(s) 111, 1466
PceI AGGCCT 2 cut(s) 1134, 2469
PciSI GCTCTTC 1 cut(s) 930
PctI GAATGC 1 cut(s) 156
PdmI GAANNNNTTC 4 cut(s) 483, 518, 915, 2391
PfeI GAWTC 7 cut(s) 5, 228, 1444, 1462, 1496, 1543, 1654
PflMI CCANNNNNTGG 1 cut(s) 633
PfoI TCCNGGA 1 cut(s) 2407
PkrI GCNGC 3 cut(s) 48, 155, 2324
PleI GAGTC 3 cut(s) 1155, 1717, 2698
PpsI GAGTC 3 cut(s) 1155, 1717, 2698
PpuMI RGGWCCY 1 cut(s) 2118
PshBI ATTAAT 2 cut(s) 2142, 2459
PsiI TTATAA 1 cut(s) 1808
Psp5II RGGWCCY 1 cut(s) 2118
Psp6I CCWGG 6 cut(s) 814, 940, 2407, 2476, 2682, 2797
PspGI CCWGG 6 cut(s) 814, 940, 2407, 2476, 2682, 2797
PspN4I GGNNCC 3 cut(s) 74, 1181, 2750
PspPI GGNCC 5 cut(s) 380, 1848, 2118, 2738, 2795
PspPPI RGGWCCY 1 cut(s) 2118
PspXI VCTCGAGB 1 cut(s) 111
PsrI GAACNNNNNNTAC 2 cut(s) 2457, 2489
PstI CTGCAG 1 cut(s) 2327
PstNI CAGNNNCTG 2 cut(s) 153, 2121
PsuI RGATCY 5 cut(s) 72, 1120, 1190, 1372, 1996
RsaI GTAC 4 cut(s) 643, 758, 1181, 2529
RsaNI GTAC 4 cut(s) 642, 757, 1180, 2528
RseI CAYNNNNRTG 2 cut(s) 1028, 2550
Rsr2I CGGWCCG 1 cut(s) 380
RsrII CGGWCCG 1 cut(s) 380
SalI GTCGAC 1 cut(s) 921
SapI GCTCTTC 1 cut(s) 930
SatI GCNGC 3 cut(s) 47, 154, 2323
Sau3AI GATC 9 cut(s) 72, 331, 775, 1120, 1190, 1372, 1915, 1996, 2695
Sau96I GGNCC 5 cut(s) 380, 1848, 2118, 2738, 2795
SchI GAGTC 3 cut(s) 1155, 1718, 2699
ScrFI CCNGG 7 cut(s) 384, 816, 942, 2409, 2478, 2684, 2799
SduI GDGCHC 1 cut(s) 2541
SfaNI GCATC 3 cut(s) 433, 1619, 2121
SfcI CTRYAG 2 cut(s) 1929, 2323
Sfr274I CTCGAG 2 cut(s) 111, 1466
SinI GGWCC 4 cut(s) 380, 1848, 2118, 2738
SlaI CTCGAG 2 cut(s) 111, 1466
SmiMI CAYNNNNRTG 2 cut(s) 1028, 2550
SmlI CTYRAG 7 cut(s) 111, 836, 1156, 1186, 1361, 1466, 2005
SmoI CTYRAG 7 cut(s) 111, 836, 1156, 1186, 1361, 1466, 2005
SphI GCATGC 1 cut(s) 85
SseBI AGGCCT 2 cut(s) 1134, 2469
SsiI CCGC 3 cut(s) 44, 85, 289
SspI AATATT 1 cut(s) 2445
SspMI CTAG 7 cut(s) 197, 713, 794, 809, 1860, 2376, 2471
StuI AGGCCT 2 cut(s) 1134, 2469
StyD4I CCNGG 7 cut(s) 382, 814, 940, 2407, 2476, 2682, 2797
StyI CCWWGG 2 cut(s) 76, 1023
TaaI ACNGT 6 cut(s) 564, 876, 927, 2543, 2735, 2759
TaiI ACGT 4 cut(s) 1119, 1816, 2550, 2830
TaqI TCGA 4 cut(s) 112, 922, 1467, 2440
TaqII GACCGA 1 cut(s) 368
TatI WGTACW 3 cut(s) 641, 756, 2527
TfiI GAWTC 7 cut(s) 5, 228, 1444, 1462, 1496, 1543, 1654
TscAI CASTG 3 cut(s) 1656, 2035, 2235
TseFI GTSAC 3 cut(s) 781, 2024, 2543
TseI GCWGC 3 cut(s) 46, 153, 2322
Tsp45I GTSAC 3 cut(s) 781, 2024, 2543
TspGWI ACGGA 2 cut(s) 570, 2399
TspRI CASTG 3 cut(s) 1656, 2035, 2235
Van91I CCANNNNNTGG 1 cut(s) 633
Vha464I CTTAAG 2 cut(s) 1186, 2005
VpaK11BI GGWCC 4 cut(s) 380, 1848, 2118, 2738
VspI ATTAAT 2 cut(s) 2142, 2459
XapI RAATTY 6 cut(s) 129, 1081, 1204, 1279, 2370, 2418
XbaI TCTAGA 1 cut(s) 1859
XceI RCATGY 1 cut(s) 85
XhoI CTCGAG 2 cut(s) 111, 1466
XmiI GTMKAC 2 cut(s) 922, 2640
XmnI GAANNNNTTC 4 cut(s) 483, 518, 915, 2391
XspI CTAG 7 cut(s) 197, 713, 794, 809, 1860, 2376, 2471
Zsp2I ATGCAT 1 cut(s) 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.