Rroxscaffold_4G00321280

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Reverse (-)
50588136 .. 50593347
5212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00321280.1

Sequence Viewer

Length: 2400 bp
ATGGCGTCAATCTCTTCTCGTCGTCAATGGAAATACGATGTGTTTCTAAGTTTCAGGGGAATAGATACGGGCAGGTCTCTAACCGATCATCTGTATTCTGCACTAAATCAGAAAGGAATTCTCACCTTTCGGGATGACCCAGAGCTTGCGAAAGGAAAATCCATTAGGCCTGAACTTTTAGCTGCAATTGAGGAGTCTAGATCTGCCATTGTCATTCTCTCACCCAGATATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAAAGCATGAAAGAGATGGGCCAACAAGTCCTCCCCGTCTTCTACTGGGTGGATCCTTCTGATGTGCGGCACCAAACGGGAAGTTTTGAGCTCAGTGTGAAACCGAAACCGAAGGTGGAAGTGAGGGAACATGAAGAAGTTTATGGGAAGAATGACAACAGACTAAATGCGTGGACAGCTGCTTTGACAGAGGTGGCCAATCTTTCTGGTTGGGTTTCCAAGAGTTATTCACATGAATCAACTTTAATTGAGGAAATTGTTGAACATATACTAAAGAAGTCCGTTTATACCACTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATTTATTAAGCAATTACATTTATCCGCAGCTTTGTGGAGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATCAAATTTGTCAGGATTTTGACCAAAGCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCATAGTTTCTCTACAAGAAAAACTTCTTTCCAAAATCCTGATTGCAAAAGTTGAAAATATAGAAGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTTGTGTAGAAAAAAGGTGCTCGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGAAGTCGCACCTGGTTTGGTACAGGGAGTAGAATTATCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTAACGGGTTAAACTGTGATGAAGCACTTAAACTTTTGAGTTTGAAGGCTTTCAAGAAATGTCCTCCTCCAGAAAATTATTTGCATCTGTGCTTCGATATTATAGGATATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCGCTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCACATAAACGAATTATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGAAGAAAAAGACAAAGAAATCTTCCTACATATCGCTTGCTTTTACAAAAATAAGGATAAGGATCGTGTGATGCAAATACTGGACTATTGCCAGCTAAACCCTGTCATTGGTTTAAGTGTTCTAGCTGATAGATCTCTCGTAACTATCTCCAACAACGAACTGTCGATGCATGATTTGCTACAAGAAACGGGCTGGGAAATTGTTCGTGAACAGTCACCCAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAGAAATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAGCTTAGTCTTCTCCATATTCGTAATGTGGATCTTCCCGACGGCCTCACTTGTCTTTCGAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTGAGATCTCTCCCACAAAATTTTGAAGTAGATGAACTTATTGAACTCAACTTGTGCCACAGCAACATTGAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACTTCGCAGGTGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGAATCCATCAATCCCTTGGATTTCTTAAAAGGCTTATTGTCCTGAATTTTAAAGACTGCAAAAGTCTTGAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAAAAGATTCCCGAGTTTGGTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTTGATGAGACTGCGATTGAGGAACTGCCTATTTCAATTGAACGGCTGACTAGCCTTGTGTCATTGAATCTAAGCAACTGCAAAAATCTTGTATGTCTTCCAAGCACCATCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTAAATGAGGGGAGACGGACTGTTTTGAGGAAACTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

799

Amino Acids

90.56

Weight (kDa)

6.7

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 191 3e-50 TIR domain
TIR_2 PF13676 14 - 107 1.8e-12 TIR domain
NB-ARC PF00931 216 - 371 6.6e-21 NB-ARC domain
AAA_22 PF13401 219 - 318 6.3e-06 AAA domain
NACHT PF05729 221 - 318 9.6e-08 NACHT domain
WHD_ROQ1 PF23282 438 - 509 3.2e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 607 - 625 2.1e-06 Leucine Rich Repeat
LRR_13 PF23286 698 - 782 8.2e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 709 - 779 2.4e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1934
Acc36I ACCTGC 2 cut(s) 63, 1934
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 1 cut(s) 1621
AciI CCGC 5 cut(s) 340, 604, 638, 679, 886
AclI AACGTT 1 cut(s) 2167
AclWI GGATC 7 cut(s) 320, 333, 661, 674, 936, 1380, 1725
AcoI YGGCCR 1 cut(s) 468
AcsI RAATTY 8 cut(s) 117, 723, 1678, 1747, 1801, 1876, 1987, 2041
AcuI CTGAAG 1 cut(s) 1607
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 979
AfeI AGCGCT 1 cut(s) 1222
AfiI CCNNNNNNNGG 3 cut(s) 1418, 1621, 2150
AjnI CCWGG 2 cut(s) 968, 1537
AjuI GAANNNNNNNTTGG 2 cut(s) 735, 767
Alw21I GWGCWC 2 cut(s) 366, 909
Alw26I GTCTC 7 cut(s) 81, 1566, 1689, 1750, 1922, 2186, 2365
AlwI GGATC 7 cut(s) 320, 333, 661, 674, 936, 1380, 1725
AlwNI CAGNNNCTG 1 cut(s) 869
Ama87I CYCGRG 2 cut(s) 705, 2144
Aor51HI AGCGCT 1 cut(s) 1222
AoxI GGCC 4 cut(s) 167, 292, 468, 1729
ApeKI GCWGC 5 cut(s) 182, 452, 640, 747, 869
ApoI RAATTY 8 cut(s) 117, 723, 1678, 1747, 1801, 1876, 1987, 2041
ArsI GACNNNNNNTTYG 2 cut(s) 439, 471
AseI ATTAAT 1 cut(s) 2108
Asp700I GAANNNNTTC 5 cut(s) 598, 810, 1103, 1512, 1606
AspLEI GCGC 1 cut(s) 1223
AspS9I GGNCC 1 cut(s) 292
AsuHPI GGTGA 3 cut(s) 115, 213, 1518
AsuII TTCGAA 1 cut(s) 1745
AvaI CYCGRG 2 cut(s) 705, 2144
BalI TGGCCA 1 cut(s) 470
BamHI GGATCC 2 cut(s) 325, 666
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 1 cut(s) 1187
BbsI GAAGAC 4 cut(s) 304, 1575, 1688, 2273
Bbv12I GWGCWC 2 cut(s) 366, 909
BbvI GCAGC 5 cut(s) 169, 439, 652, 734, 856
BccI CCATC 4 cut(s) 283, 1298, 2010, 2300
BceAI ACGGC 2 cut(s) 1744, 2243
BciT130I CCWGG 2 cut(s) 970, 1539
BciVI GTATCC 1 cut(s) 1788
BclI TGATCA 1 cut(s) 718
BcoDI GTCTC 7 cut(s) 81, 1566, 1689, 1750, 1922, 2186, 2365
BfaI CTAG 3 cut(s) 198, 1433, 2235
BfoI RGCGCY 1 cut(s) 1224
BfuAI ACCTGC 2 cut(s) 63, 1934
BfuI GTATCC 1 cut(s) 1788
BglII AGATCT 4 cut(s) 200, 1442, 1787, 1969
BisI GCNGC 6 cut(s) 183, 341, 453, 641, 748, 870
BlsI GCNGC 6 cut(s) 184, 342, 454, 642, 749, 871
Bme1390I CCNGG 2 cut(s) 970, 1539
BmeT110I CYCGRG 2 cut(s) 705, 2144
BmgT120I GGNCC 1 cut(s) 292
BmiI GGNNCC 3 cut(s) 327, 344, 668
BmrFI CCNGG 2 cut(s) 970, 1539
BmrI ACTGGG 2 cut(s) 328, 1782
BmsI GCATC 5 cut(s) 1030, 1147, 1371, 1467, 2324
BmuI ACTGGG 2 cut(s) 328, 1782
BpiI GAAGAC 4 cut(s) 304, 1575, 1688, 2273
BpmI CTGGAG 1 cut(s) 1107
Bpu10I CCTNAGC 1 cut(s) 1167
Bpu14I TTCGAA 1 cut(s) 1745
BpuEI CTTGAG 4 cut(s) 1774, 1804, 1979, 2084
BsaBI GATNNNNATC 2 cut(s) 927, 1371
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 3 cut(s) 81, 1566, 1922
BsaJI CCNNGG 3 cut(s) 670, 1615, 2011
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 3 cut(s) 1418, 1621, 2150
Bse1I ACTGG 4 cut(s) 323, 1314, 1395, 1777
Bse8I GATNNNNATC 2 cut(s) 927, 1371
BseBI CCWGG 2 cut(s) 970, 1539
BseDI CCNNGG 3 cut(s) 670, 1615, 2011
BseGI GGATG 4 cut(s) 139, 259, 1985, 2339
BseJI GATNNNNATC 2 cut(s) 927, 1371
BseLI CCNNNNNNNGG 3 cut(s) 1418, 1621, 2150
BseMII CTCAG 3 cut(s) 379, 1181, 1700
BseNI ACTGG 4 cut(s) 323, 1314, 1395, 1777
BseRI GAGGAG 3 cut(s) 206, 1110, 1751
BseXI GCAGC 5 cut(s) 169, 439, 652, 734, 856
BseYI CCCAGC 1 cut(s) 1503
BsgI GTGCAG 1 cut(s) 84
BshFI GGCC 4 cut(s) 169, 294, 470, 1731
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 2 cut(s) 366, 909
BsiHKCI CYCGRG 2 cut(s) 705, 2144
BslI CCNNNNNNNGG 3 cut(s) 1418, 1621, 2150
BsmAI GTCTC 7 cut(s) 81, 1566, 1689, 1750, 1922, 2186, 2365
BsmBI CGTCTC 1 cut(s) 2365
BsnI GGCC 4 cut(s) 169, 294, 470, 1731
Bso31I GGTCTC 3 cut(s) 81, 1566, 1922
BsoBI CYCGRG 2 cut(s) 705, 2144
Bsp119I TTCGAA 1 cut(s) 1745
Bsp1286I GDGCHC 2 cut(s) 366, 909
Bsp19I CCATGG 1 cut(s) 670
BspACI CCGC 5 cut(s) 340, 604, 638, 679, 886
BspANI GGCC 4 cut(s) 169, 294, 470, 1731
BspCNI CTCAG 3 cut(s) 378, 1180, 1699
BspHI TCATGA 1 cut(s) 1564
BspLI GGNNCC 3 cut(s) 327, 344, 668
BspMI ACCTGC 2 cut(s) 63, 1934
BspPI GGATC 7 cut(s) 320, 333, 661, 674, 936, 1380, 1725
BspT104I TTCGAA 1 cut(s) 1745
BspT107I GGYRCC 1 cut(s) 342
BspTNI GGTCTC 3 cut(s) 81, 1566, 1922
BsrI ACTGG 4 cut(s) 323, 1314, 1395, 1777
BssECI CCNNGG 3 cut(s) 670, 1615, 2011
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1187
BssT1I CCWWGG 3 cut(s) 670, 1615, 2011
Bst2BI CACGAG 1 cut(s) 1187
Bst2UI CCWGG 2 cut(s) 970, 1539
Bst4CI ACNGT 4 cut(s) 1070, 1473, 1524, 2380
Bst6I CTCTTC 2 cut(s) 19, 1583
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1486
BstBI TTCGAA 1 cut(s) 1745
BstC8I GCNNGC 4 cut(s) 147, 677, 1348, 1403
BstDEI CTNAG 6 cut(s) 47, 365, 1167, 1686, 1691, 2255
BstDSI CCRYGG 1 cut(s) 670
BstF5I GGATG 4 cut(s) 139, 259, 1985, 2339
BstH2I RGCGCY 1 cut(s) 1224
BstHHI GCGC 1 cut(s) 1223
BstMAI GTCTC 7 cut(s) 81, 1566, 1689, 1750, 1922, 2186, 2365
BstMWI GCNNNNNNNGC 3 cut(s) 449, 681, 1486
BstNI CCWGG 2 cut(s) 970, 1539
BstNSI RCATGY 1 cut(s) 679
BstSCI CCNGG 2 cut(s) 968, 1537
BstV1I GCAGC 5 cut(s) 169, 439, 652, 734, 856
BstV2I GAAGAC 4 cut(s) 304, 1575, 1688, 2273
BstX2I RGATCY 7 cut(s) 200, 325, 666, 1442, 1717, 1787, 1969
BstYI RGATCY 7 cut(s) 200, 325, 666, 1442, 1717, 1787, 1969
BsuI GTATCC 1 cut(s) 1788
BsuRI GGCC 4 cut(s) 169, 294, 470, 1731
BtgI CCRYGG 1 cut(s) 670
BtsCI GGATG 4 cut(s) 139, 259, 1985, 2339
BtsIMutI CAGTG 1 cut(s) 373
BveI ACCTGC 2 cut(s) 63, 1934
Cac8I GCNNGC 4 cut(s) 147, 677, 1348, 1403
CaiI CAGNNNCTG 1 cut(s) 869
CciI TCATGA 1 cut(s) 1564
CfoI GCGC 1 cut(s) 1223
Cfr13I GGNCC 1 cut(s) 292
CsiI ACCWGGT 1 cut(s) 968
Csp6I GTAC 1 cut(s) 978
CviQI GTAC 1 cut(s) 978
DdeI CTNAG 6 cut(s) 47, 365, 1167, 1686, 1691, 2255
DraI TTTAAA 1 cut(s) 2047
EaeI YGGCCR 1 cut(s) 468
Eam1104I CTCTTC 2 cut(s) 19, 1583
EarI CTCTTC 2 cut(s) 19, 1583
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 3 cut(s) 670, 1615, 2011
Eco147I AGGCCT 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 3 cut(s) 81, 1566, 1922
Eco47III AGCGCT 1 cut(s) 1222
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 1 cut(s) 1607
Eco88I CYCGRG 2 cut(s) 705, 2144
EcoICRI GAGCTC 1 cut(s) 364
EcoRI GAATTC 2 cut(s) 117, 1747
EcoRII CCWGG 2 cut(s) 968, 1537
EcoT14I CCWWGG 3 cut(s) 670, 1615, 2011
EcoT22I ATGCAT 1 cut(s) 1482
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 3 cut(s) 670, 1615, 2011
Esp3I CGTCTC 1 cut(s) 2365
FalI AAGNNNNNCTT 2 cut(s) 795, 827
FauI CCCGC 2 cut(s) 611, 672
FbaI TGATCA 1 cut(s) 718
Fnu4HI GCNGC 6 cut(s) 183, 341, 453, 641, 748, 870
FokI GGATG 4 cut(s) 146, 266, 1992, 2346
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 6 cut(s) 183, 341, 453, 641, 748, 870
FspBI CTAG 3 cut(s) 198, 1433, 2235
GlaI GCGC 1 cut(s) 1222
GluI GCNGC 6 cut(s) 183, 341, 453, 641, 748, 870
GsaI CCCAGC 1 cut(s) 1507
GsuI CTGGAG 1 cut(s) 1107
HaeII RGCGCY 1 cut(s) 1224
HaeIII GGCC 4 cut(s) 169, 294, 470, 1731
HhaI GCGC 1 cut(s) 1223
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 1221
HinP1I GCGC 1 cut(s) 1221
HincII GTYRAC 3 cut(s) 577, 936, 1635
HindII GTYRAC 3 cut(s) 577, 936, 1635
HphI GGTGA 3 cut(s) 115, 213, 1518
Hpy166II GTNNAC 5 cut(s) 447, 577, 936, 1520, 1635
Hpy188I TCNGA 4 cut(s) 111, 334, 1293, 1953
Hpy8I GTNNAC 5 cut(s) 447, 577, 936, 1520, 1635
Hpy99I CGWCG 2 cut(s) 24, 1730
HpyAV CCTTC 5 cut(s) 339, 379, 1093, 1858, 1970
HpyCH4III ACNGT 4 cut(s) 1070, 1473, 1524, 2380
HpyCH4IV ACGT 2 cut(s) 1546, 2167
HpyCH4V TGCA 9 cut(s) 101, 185, 833, 1138, 1241, 1384, 1480, 2055, 2265
HpyF10VI GCNNNNNNNGC 3 cut(s) 449, 681, 1486
HpyF3I CTNAG 6 cut(s) 47, 365, 1167, 1686, 1691, 2255
HpySE526I ACGT 2 cut(s) 1546, 2167
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 1221
Ksp22I TGATCA 1 cut(s) 718
LmnI GCTCC 1 cut(s) 866
Lsp1109I GCAGC 5 cut(s) 169, 439, 652, 734, 856
LweI GCATC 5 cut(s) 1030, 1147, 1371, 1467, 2324
MabI ACCWGGT 1 cut(s) 968
MaeI CTAG 3 cut(s) 198, 1433, 2235
MaeII ACGT 2 cut(s) 1546, 2167
MaeIII GTNAC 2 cut(s) 1450, 1524
MfeI CAATTG 2 cut(s) 186, 2220
MflI RGATCY 7 cut(s) 200, 325, 666, 1442, 1717, 1787, 1969
MhlI GDGCHC 2 cut(s) 366, 909
MlsI TGGCCA 1 cut(s) 470
MluNI TGGCCA 1 cut(s) 470
MlyI GAGTC 3 cut(s) 203, 1752, 2077
MmeI TCCRAC 1 cut(s) 1485
Mox20I TGGCCA 1 cut(s) 470
Mph1103I ATGCAT 1 cut(s) 1482
MroXI GAANNNNTTC 5 cut(s) 598, 810, 1103, 1512, 1606
MscI TGGCCA 1 cut(s) 470
MslI CAYNNNNRTG 2 cut(s) 593, 1625
Msp20I TGGCCA 1 cut(s) 470
MspA1I CMGCKG 1 cut(s) 452
MspR9I CCNGG 2 cut(s) 970, 1539
MunI CAATTG 2 cut(s) 186, 2220
MvaI CCWGG 2 cut(s) 970, 1539
MwoI GCNNNNNNNGC 3 cut(s) 449, 681, 1486
NcoI CCATGG 1 cut(s) 670
NlaIV GGNNCC 3 cut(s) 327, 344, 668
NmeAIII GCCGAG 1 cut(s) 2325
NmuCI GTSAC 1 cut(s) 1524
NsiI ATGCAT 1 cut(s) 1482
NspI RCATGY 1 cut(s) 679
NspV TTCGAA 1 cut(s) 1745
PaeI GCATGC 1 cut(s) 679
PaeR7I CTCGAG 1 cut(s) 705
PagI TCATGA 1 cut(s) 1564
PaqCI CACCTGC 1 cut(s) 1934
PceI AGGCCT 1 cut(s) 169
PdmI GAANNNNTTC 5 cut(s) 598, 810, 1103, 1512, 1606
PfeI GAWTC 8 cut(s) 509, 599, 1607, 1954, 1998, 2093, 2140, 2251
PflMI CCANNNNNTGG 1 cut(s) 1621
PkrI GCNGC 6 cut(s) 184, 342, 454, 642, 749, 871
PleI GAGTC 3 cut(s) 202, 1752, 2076
PpsI GAGTC 3 cut(s) 202, 1752, 2076
PshBI ATTAAT 1 cut(s) 2108
Psp124BI GAGCTC 1 cut(s) 366
Psp1406I AACGTT 1 cut(s) 2167
Psp6I CCWGG 2 cut(s) 968, 1537
PspFI CCCAGC 1 cut(s) 1503
PspGI CCWGG 2 cut(s) 968, 1537
PspN4I GGNNCC 3 cut(s) 327, 344, 668
PspPI GGNCC 1 cut(s) 292
PspXI VCTCGAGB 1 cut(s) 705
PstNI CAGNNNCTG 1 cut(s) 869
PsuI RGATCY 7 cut(s) 200, 325, 666, 1442, 1717, 1787, 1969
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 1 cut(s) 979
RsaNI GTAC 1 cut(s) 978
RseI CAYNNNNRTG 2 cut(s) 593, 1625
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 6 cut(s) 183, 341, 453, 641, 748, 870
Sau96I GGNCC 1 cut(s) 292
SchI GAGTC 3 cut(s) 203, 1752, 2077
ScrFI CCNGG 2 cut(s) 970, 1539
SduI GDGCHC 2 cut(s) 366, 909
SexAI ACCWGGT 1 cut(s) 968
SfaNI GCATC 5 cut(s) 1030, 1147, 1371, 1467, 2324
Sfr274I CTCGAG 1 cut(s) 705
SfuI TTCGAA 1 cut(s) 1745
SlaI CTCGAG 1 cut(s) 705
SmiMI CAYNNNNRTG 2 cut(s) 593, 1625
SmlI CTYRAG 5 cut(s) 705, 1753, 1783, 1958, 2063
SmoI CTYRAG 5 cut(s) 705, 1753, 1783, 1958, 2063
SphI GCATGC 1 cut(s) 679
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 5 cut(s) 340, 604, 638, 679, 886
SspMI CTAG 3 cut(s) 198, 1433, 2235
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 2 cut(s) 968, 1537
StyI CCWWGG 3 cut(s) 670, 1615, 2011
TaaI ACNGT 4 cut(s) 1070, 1473, 1524, 2380
TaiI ACGT 2 cut(s) 1549, 2170
TaqI TCGA 5 cut(s) 706, 1149, 1475, 1745, 1764
TauI GCSGC 1 cut(s) 343
TfiI GAWTC 8 cut(s) 509, 599, 1607, 1954, 1998, 2093, 2140, 2251
TscAI CASTG 1 cut(s) 373
TseFI GTSAC 1 cut(s) 1524
TseI GCWGC 5 cut(s) 182, 452, 640, 747, 869
Tsp45I GTSAC 1 cut(s) 1524
TspGWI ACGGA 3 cut(s) 544, 1167, 2389
TspRI CASTG 1 cut(s) 373
Van91I CCANNNNNTGG 1 cut(s) 1621
VspI ATTAAT 1 cut(s) 2108
XapI RAATTY 8 cut(s) 117, 723, 1678, 1747, 1801, 1876, 1987, 2041
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 1 cut(s) 679
XcmI CCANNNNNNNNNTGG 1 cut(s) 2009
XhoI CTCGAG 1 cut(s) 705
XmnI GAANNNNTTC 5 cut(s) 598, 810, 1103, 1512, 1606
XspI CTAG 3 cut(s) 198, 1433, 2235
Zsp2I ATGCAT 1 cut(s) 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.