Rroxscaffold_4G00326530

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Reverse (-)
58701458 .. 58705566
4109 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00326530.1

Sequence Viewer

Length: 1704 bp
ATGGAAATACGTTGTCTTTCTAAGTTTCAGGGGGAAGATACCCGCTATGGTTTTACAAATGATCTGTATACTGCACTAAATCGGAAAGGAATTCTGACCATTATGGATGACGCAGAGCTTGAGAAAGGAAAATCCATTAGCCCTGAACTTTTTGGTGCAATTGAGGACTCTAGATATGCTATTGTTCATTCTCTCACTCAACTATGCTTCTTCGTCATGGGGAAGGATAAGGATCGTGTGACACAAATACTAGAGTATTGCCAGCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATCGATGTCTCATAACTATCTCCAACAACGAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAATCAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAAGAGACATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTGAATTACAAGTGGCTCATTGGAAGCCAGAATCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTCGTAATGTGGACCTTCCCGAAGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTAAGATCTCTCCCACAAAATTTTGAAGCAGATGAACTTATTGAACTTAACTTGTGCCACGGCAACATTGAACAGCTTTGGAATGGAACAAAGATGGACTGTTTTGAGGAAACTGATGTGAATAGTGGGTATGCAATAGAAGTCTCATCTGCCCGTGATCGCATAAAATATGTGAGAGGAGCATTTCCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGGAACATTTTTGTTCGTCTTCCTTCAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAACACTTGTTTGATGCATCAAATTTGAAGGGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCAGTTGAAATGCTGAAGATATTCAGTTTCAGGGAGACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCAGAGTGTTGGGTGTTCCTTAAGGGTACCTACACCTGAATATTGGAACAACAGGAGGGTTTTGGGATTTGCTTTGTGTTTTGTTTTTGTACTCCATGAGCACCATCGGGTCGATGAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTTAAGTCAGATCACCTGTGGCTATTCTATGTATCTAGTGGTAAATACTTTCATACAGATTGGCGGCATACATTTTGCGGTCGGCTTGAGTTCTTATTTGAAACCAGAGGGCCAGGTCTGAAGGTGAAGGAGTATGGAGTCCATCTGATATATAAGCACGATGTGCAAAAGTTGAACCGAACAACCACTCAATCAAGCAGTTGGATGTCTCCAGTTGCAGGGGAAACCAGTGGCACTGGTAGCAGAACTTGCACGCTTGAAGAATTGCAGGATTGGCAGAATATAGGGGATCTACCACTGGCTACTCTGAAAGACGTCCACAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

567

Amino Acids

64.9

Weight (kDa)

5.81

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 7 - 62 4.6e-10 TIR domain
WHD_ROQ1 PF23282 69 - 129 2.9e-14 Disease resistance protein Roq1-like, winged-helix domain
C-JID PF20160 394 - 515 2e-16 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1695
Acc65I GGTACC 1 cut(s) 1240
AccB1I GGYRCC 1 cut(s) 1240
AccB7I CCANNNNNTGG 1 cut(s) 481
AccI GTMKAC 2 cut(s) 68, 991
AciI CCGC 3 cut(s) 43, 1441, 1455
AclWI GGATC 2 cut(s) 240, 1674
AcsI RAATTY 6 cut(s) 90, 538, 661, 836, 1048, 1346
AcuI CTGAAG 4 cut(s) 467, 885, 1163, 1517
AcyI GRCGYC 1 cut(s) 1692
AdeI CACNNNGTG 1 cut(s) 1540
AfaI GTAC 3 cut(s) 1018, 1242, 1305
AfiI CCNNNNNNNGG 3 cut(s) 278, 481, 1595
AflII CTTAAG 2 cut(s) 643, 1234
AjnI CCWGG 3 cut(s) 397, 1183, 1489
AjuI GAANNNNNNNTTGG 2 cut(s) 923, 955
AluBI AGCT 5 cut(s) 118, 265, 718, 925, 1333
AluI AGCT 5 cut(s) 118, 265, 718, 925, 1333
Alw21I GWGCWC 1 cut(s) 1317
AlwI GGATC 2 cut(s) 240, 1674
AoxI GGCC 2 cut(s) 589, 1487
ApoI RAATTY 6 cut(s) 90, 538, 661, 836, 1048, 1346
Asp700I GAANNNNTTC 3 cut(s) 372, 466, 1148
Asp718I GGTACC 1 cut(s) 1240
AspS9I GGNCC 2 cut(s) 577, 1487
AsuHPI GGTGA 3 cut(s) 871, 1382, 1513
AvaII GGWCC 1 cut(s) 577
BanI GGYRCC 1 cut(s) 1240
BbsI GAAGAC 3 cut(s) 435, 548, 887
Bbv12I GWGCWC 1 cut(s) 1317
BccI CCATC 6 cut(s) 730, 988, 1004, 1221, 1326, 1527
BceAI ACGGC 1 cut(s) 718
BciT130I CCWGG 3 cut(s) 399, 1185, 1491
BciVI GTATCC 1 cut(s) 648
BfaI CTAG 4 cut(s) 171, 251, 995, 1413
BfrI CTTAAG 2 cut(s) 643, 1234
BfuI GTATCC 1 cut(s) 648
BglII AGATCT 1 cut(s) 647
BisI GCNGC 1 cut(s) 1442
BlsI GCNGC 1 cut(s) 1443
Bme1390I CCNGG 3 cut(s) 399, 1185, 1491
Bme18I GGWCC 1 cut(s) 577
BmgT120I GGNCC 2 cut(s) 577, 1487
BmiI GGNNCC 1 cut(s) 1242
BmrFI CCNGG 3 cut(s) 399, 1185, 1491
BmrI ACTGGG 1 cut(s) 642
BmsI GCATC 4 cut(s) 327, 1030, 1052, 1106
BmuI ACTGGG 1 cut(s) 642
BpiI GAAGAC 3 cut(s) 435, 548, 887
BpmI CTGGAG 1 cut(s) 1572
BpuEI CTTGAG 4 cut(s) 140, 634, 947, 1484
Bsa29I ATCGAT 1 cut(s) 301
BsaBI GATNNNNATC 1 cut(s) 231
BsaHI GRCGYC 1 cut(s) 1692
BsaI GGTCTC 1 cut(s) 426
BsaJI CCNNGG 2 cut(s) 475, 700
Bsc4I CCNNNNNNNGG 3 cut(s) 278, 481, 1595
Bse1I ACTGG 5 cut(s) 637, 1589, 1605, 1618, 1680
Bse8I GATNNNNATC 1 cut(s) 231
BseBI CCWGG 3 cut(s) 399, 1185, 1491
BseCI ATCGAT 1 cut(s) 301
BseDI CCNNGG 2 cut(s) 475, 700
BseGI GGATG 3 cut(s) 112, 1121, 1587
BseJI GATNNNNATC 1 cut(s) 231
BseLI CCNNNNNNNGG 3 cut(s) 278, 481, 1595
BseMII CTCAG 1 cut(s) 1191
BseNI ACTGG 5 cut(s) 637, 1589, 1605, 1618, 1680
BseRI GAGGAG 2 cut(s) 611, 834
BseYI CCCAGC 1 cut(s) 363
BsgI GTGCAG 1 cut(s) 57
Bsh1285I CGRYCG 1 cut(s) 1459
BshFI GGCC 2 cut(s) 591, 1489
BshNI GGYRCC 1 cut(s) 1240
BshVI ATCGAT 1 cut(s) 301
BsiEI CGRYCG 1 cut(s) 1459
BsiHKAI GWGCWC 1 cut(s) 1317
BslI CCNNNNNNNGG 3 cut(s) 278, 481, 1595
BsnI GGCC 2 cut(s) 591, 1489
Bso31I GGTCTC 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 1317
Bsp143I GATC 7 cut(s) 61, 232, 298, 647, 799, 1387, 1666
BspACI CCGC 3 cut(s) 43, 1441, 1455
BspANI GGCC 2 cut(s) 591, 1489
BspCNI CTCAG 1 cut(s) 1192
BspDI ATCGAT 1 cut(s) 301
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 1 cut(s) 1242
BspPI GGATC 2 cut(s) 240, 1674
BspT107I GGYRCC 1 cut(s) 1240
BspTI CTTAAG 2 cut(s) 643, 1234
BspTNI GGTCTC 1 cut(s) 426
BsrI ACTGG 5 cut(s) 637, 1589, 1605, 1618, 1680
BssECI CCNNGG 2 cut(s) 475, 700
BssMI GATC 7 cut(s) 61, 232, 298, 647, 799, 1387, 1666
BssNAI GTATAC 2 cut(s) 69, 992
BssNI GRCGYC 1 cut(s) 1692
BssT1I CCWWGG 1 cut(s) 475
Bst1107I GTATAC 2 cut(s) 69, 992
Bst2UI CCWGG 3 cut(s) 399, 1185, 1491
Bst4CI ACNGT 3 cut(s) 333, 384, 743
Bst6I CTCTTC 1 cut(s) 443
BstACI GRCGYC 1 cut(s) 1692
BstAFI CTTAAG 2 cut(s) 643, 1234
BstAPI GCANNNNNTGC 3 cut(s) 346, 1540, 1626
BstC8I GCNNGC 2 cut(s) 263, 1631
BstDEI CTNAG 5 cut(s) 21, 551, 872, 921, 1200
BstDSI CCRYGG 1 cut(s) 700
BstEII GGTNACC 1 cut(s) 859
BstF5I GGATG 3 cut(s) 112, 1121, 1587
BstKTI GATC 7 cut(s) 64, 235, 301, 650, 802, 1390, 1669
BstMBI GATC 7 cut(s) 61, 232, 298, 647, 799, 1387, 1666
BstMCI CGRYCG 1 cut(s) 1459
BstMWI GCNNNNNNNGC 6 cut(s) 346, 922, 1540, 1617, 1626, 1651
BstNI CCWGG 3 cut(s) 399, 1185, 1491
BstPI GGTNACC 1 cut(s) 859
BstSCI CCNGG 3 cut(s) 397, 1183, 1489
BstV2I GAAGAC 3 cut(s) 435, 548, 887
BstX2I RGATCY 2 cut(s) 647, 1666
BstYI RGATCY 2 cut(s) 647, 1666
BstZ17I GTATAC 2 cut(s) 69, 992
Bsu15I ATCGAT 1 cut(s) 301
BsuI GTATCC 1 cut(s) 648
BsuRI GGCC 2 cut(s) 591, 1489
BsuTUI ATCGAT 1 cut(s) 301
BtgI CCRYGG 1 cut(s) 700
BtsCI GGATG 3 cut(s) 112, 1121, 1587
BtsIMutI CAGTG 4 cut(s) 1022, 1611, 1612, 1673
Cac8I GCNNGC 2 cut(s) 263, 1631
CciI TCATGA 1 cut(s) 424
Cfr13I GGNCC 2 cut(s) 577, 1487
ClaI ATCGAT 1 cut(s) 301
CseI GACGC 1 cut(s) 119
Csp6I GTAC 3 cut(s) 1017, 1241, 1304
CviAII CATG 5 cut(s) 217, 341, 425, 481, 1310
CviQI GTAC 3 cut(s) 1017, 1241, 1304
DdeI CTNAG 5 cut(s) 21, 551, 872, 921, 1200
DpnI GATC 7 cut(s) 63, 234, 300, 649, 801, 1389, 1668
DpnII GATC 7 cut(s) 61, 232, 298, 647, 799, 1387, 1666
DraIII CACNNNGTG 1 cut(s) 1540
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
Eco130I CCWWGG 1 cut(s) 475
Eco147I AGGCCT 1 cut(s) 591
Eco31I GGTCTC 1 cut(s) 426
Eco47I GGWCC 1 cut(s) 577
Eco57I CTGAAG 4 cut(s) 467, 885, 1163, 1517
Eco91I GGTNACC 1 cut(s) 859
EcoO65I GGTNACC 1 cut(s) 859
EcoRI GAATTC 2 cut(s) 90, 1346
EcoRII CCWGG 3 cut(s) 397, 1183, 1489
EcoT14I CCWWGG 1 cut(s) 475
EcoT22I ATGCAT 2 cut(s) 342, 1045
ErhI CCWWGG 1 cut(s) 475
FaeI CATG 5 cut(s) 220, 344, 428, 484, 1313
FatI CATG 5 cut(s) 216, 340, 424, 480, 1309
FauI CCCGC 1 cut(s) 50
FblI GTMKAC 2 cut(s) 68, 991
Fnu4HI GCNGC 1 cut(s) 1442
FokI GGATG 3 cut(s) 119, 1128, 1594
Fsp4HI GCNGC 1 cut(s) 1442
FspBI CTAG 4 cut(s) 171, 251, 995, 1413
GluI GCNGC 1 cut(s) 1442
GsaI CCCAGC 1 cut(s) 367
GsuI CTGGAG 1 cut(s) 1572
HaeIII GGCC 2 cut(s) 591, 1489
HgaI GACGC 1 cut(s) 119
Hin1I GRCGYC 1 cut(s) 1692
Hin1II CATG 5 cut(s) 220, 344, 428, 484, 1313
HincII GTYRAC 1 cut(s) 495
HindII GTYRAC 1 cut(s) 495
HindIII AAGCTT 1 cut(s) 923
HinfI GANTC 6 cut(s) 167, 467, 527, 618, 868, 1515
HphI GGTGA 3 cut(s) 871, 1382, 1513
Hpy166II GTNNAC 6 cut(s) 69, 380, 495, 577, 992, 1696
Hpy188I TCNGA 8 cut(s) 84, 96, 973, 1218, 1387, 1497, 1524, 1686
Hpy188III TCNNGA 7 cut(s) 171, 377, 425, 584, 962, 1199, 1351
Hpy8I GTNNAC 6 cut(s) 69, 380, 495, 577, 992, 1696
HpyAV CCTTC 8 cut(s) 217, 581, 590, 909, 986, 1048, 1492, 1498
HpyCH4III ACNGT 3 cut(s) 333, 384, 743
HpyCH4IV ACGT 3 cut(s) 10, 406, 1692
HpyF10VI GCNNNNNNNGC 6 cut(s) 346, 922, 1540, 1617, 1626, 1651
HpyF3I CTNAG 5 cut(s) 21, 551, 872, 921, 1200
HpySE526I ACGT 3 cut(s) 10, 406, 1692
Hsp92I GRCGYC 1 cut(s) 1692
Hsp92II CATG 5 cut(s) 220, 344, 428, 484, 1313
KpnI GGTACC 1 cut(s) 1244
Kzo9I GATC 7 cut(s) 61, 232, 298, 647, 799, 1387, 1666
LmnI GCTCC 1 cut(s) 821
LweI GCATC 4 cut(s) 327, 1030, 1052, 1106
MaeI CTAG 4 cut(s) 171, 251, 995, 1413
MaeII ACGT 3 cut(s) 10, 406, 1692
MaeIII GTNAC 3 cut(s) 238, 842, 859
MalI GATC 7 cut(s) 63, 234, 300, 649, 801, 1389, 1668
MboI GATC 7 cut(s) 61, 232, 298, 647, 799, 1387, 1666
MboII GAAGA 8 cut(s) 47, 202, 440, 460, 548, 887, 1156, 1649
MfeI CAATTG 2 cut(s) 159, 1087
MflI RGATCY 2 cut(s) 647, 1666
MhlI GDGCHC 1 cut(s) 1317
MlyI GAGTC 3 cut(s) 161, 612, 1524
MmeI TCCRAC 2 cut(s) 345, 1559
MnlI CCTC 8 cut(s) 157, 602, 632, 742, 812, 862, 1263, 1478
Mph1103I ATGCAT 2 cut(s) 342, 1045
MroXI GAANNNNTTC 3 cut(s) 372, 466, 1148
MslI CAYNNNNRTG 2 cut(s) 485, 1218
MspCI CTTAAG 2 cut(s) 643, 1234
MspR9I CCNGG 3 cut(s) 399, 1185, 1491
MunI CAATTG 2 cut(s) 159, 1087
MvaI CCWGG 3 cut(s) 399, 1185, 1491
MwoI GCNNNNNNNGC 6 cut(s) 346, 922, 1540, 1617, 1626, 1651
NdeII GATC 7 cut(s) 61, 232, 298, 647, 799, 1387, 1666
NlaIII CATG 5 cut(s) 220, 344, 428, 484, 1313
NlaIV GGNNCC 1 cut(s) 1242
NmuCI GTSAC 2 cut(s) 238, 859
NsiI ATGCAT 2 cut(s) 342, 1045
PagI TCATGA 1 cut(s) 424
PceI AGGCCT 1 cut(s) 591
PdmI GAANNNNTTC 3 cut(s) 372, 466, 1148
PfeI GAWTC 3 cut(s) 467, 527, 868
PflMI CCANNNNNTGG 1 cut(s) 481
PfoI TCCNGGA 1 cut(s) 1183
PkrI GCNGC 1 cut(s) 1443
PleI GAGTC 3 cut(s) 161, 612, 1523
PpsI GAGTC 3 cut(s) 161, 612, 1523
Psp6I CCWGG 3 cut(s) 397, 1183, 1489
PspEI GGTNACC 1 cut(s) 859
PspFI CCCAGC 1 cut(s) 363
PspGI CCWGG 3 cut(s) 397, 1183, 1489
PspN4I GGNNCC 1 cut(s) 1242
PspPI GGNCC 2 cut(s) 577, 1487
PsuI RGATCY 2 cut(s) 647, 1666
RsaI GTAC 3 cut(s) 1018, 1242, 1305
RsaNI GTAC 3 cut(s) 1017, 1241, 1304
RseI CAYNNNNRTG 2 cut(s) 485, 1218
SatI GCNGC 1 cut(s) 1442
Sau3AI GATC 7 cut(s) 61, 232, 298, 647, 799, 1387, 1666
Sau96I GGNCC 2 cut(s) 577, 1487
SchI GAGTC 3 cut(s) 161, 612, 1524
ScrFI CCNGG 3 cut(s) 399, 1185, 1491
SduI GDGCHC 1 cut(s) 1317
SfaNI GCATC 4 cut(s) 327, 1030, 1052, 1106
SinI GGWCC 1 cut(s) 577
SmiMI CAYNNNNRTG 2 cut(s) 485, 1218
SmlI CTYRAG 6 cut(s) 119, 613, 643, 926, 1234, 1463
SmoI CTYRAG 6 cut(s) 119, 613, 643, 926, 1234, 1463
SseBI AGGCCT 1 cut(s) 591
SsiI CCGC 3 cut(s) 43, 1441, 1455
SspI AATATT 2 cut(s) 1195, 1256
SspMI CTAG 4 cut(s) 171, 251, 995, 1413
StuI AGGCCT 1 cut(s) 591
StyD4I CCNGG 3 cut(s) 397, 1183, 1489
StyI CCWWGG 1 cut(s) 475
TaaI ACNGT 3 cut(s) 333, 384, 743
TaiI ACGT 3 cut(s) 13, 409, 1695
TaqI TCGA 4 cut(s) 301, 335, 624, 1326
TatI WGTACW 2 cut(s) 1016, 1303
TauI GCSGC 1 cut(s) 1444
TfiI GAWTC 3 cut(s) 467, 527, 868
TscAI CASTG 4 cut(s) 1029, 1612, 1618, 1680
TseFI GTSAC 2 cut(s) 238, 859
Tsp45I GTSAC 2 cut(s) 238, 859
TspDTI ATGAA 6 cut(s) 176, 441, 690, 1072, 1359, 1418
TspRI CASTG 4 cut(s) 1029, 1612, 1618, 1680
Van91I CCANNNNNTGG 1 cut(s) 481
Vha464I CTTAAG 2 cut(s) 643, 1234
VpaK11BI GGWCC 1 cut(s) 577
XapI RAATTY 6 cut(s) 90, 538, 661, 836, 1048, 1346
XbaI TCTAGA 1 cut(s) 170
XcmI CCANNNNNNNNNTGG 1 cut(s) 1220
XmiI GTMKAC 2 cut(s) 68, 991
XmnI GAANNNNTTC 3 cut(s) 372, 466, 1148
XspI CTAG 4 cut(s) 171, 251, 995, 1413
ZraI GACGTC 1 cut(s) 1693
Zsp2I ATGCAT 2 cut(s) 342, 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.