Rroxscaffold_1G00024620

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000105
Physical Location & Seq
Reverse (-)
30599959 .. 30607306
7348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00024620.1

Sequence Viewer

Length: 3153 bp
ATGCTATGCAAGAAAAAGATGGGACAAATAGTCATTCCGGTCTTCTACGAAGTGGATCCTTCTGATGTGCGGCACCAAACGGGAAGTTTTCAGCTCCGTAGGAAATTAAAAGTGGAAGTAATGGAACACGAAGAAGTATATGGGGAGAATGAGGATAAGTTGAAGGAGTGGGCAGCTGCTTTGACAGAGCTGGCCAATCTTTCTGGCTGGCATTCCCAGAGTGGAGATGATAGAGAATTGATTGAGGACATTGTTCATGATCAAGTTCGAAAGTTATTTCATTTAGAATCTTCAAGTGCTGACAAGGGTTTCGTAGGAATGGATTCCCGCATTGGTGATTTGTTGCACAATTATATTTGTCCACAGTTTGGTGGTGTTCGTATTATAGGGATCCATGGCATGCGAGGAATAGGCAAGACAACACTTGCTGTAGCTATTCATGATGAAATCTGTCAGGATTTTGACCGAAGCTGCTTTCTTTCTGATGTTAGAGAAAGTTCCAAAAAGGATGGCCTAGTTTCTCTACAAAAAAAACTTCTTTTCAGTATCCTGGGGGCAAATATTGACATACAGAATGAATATGCAGGAGCTACTATGATCAAAAGGCGGTTATGTAAAATAAAGGTGCTTATTGTTATTGATGATGTGGATGACATCAATCAATTAGAGAAATTGGCAGGAAGTCGTGACTGGTTTGGTCCAGAGAGTAGAATTGTTGTAACCACCCCAGATATTCATTTGCTAATCACACATGATGTTGATGCTACATACAAGGCTGCTGGGTTAAATGGTCATGAAGCTATTGAACTATTGAGTTTAAAGGCTTTCAAGAAAAGCGTCCCACCAGCAGATCATTCGAAGTTGTGCAAACTTATTTTAGCGTATGCTCAAGGGCTTCCATTGGCTGTTGTGGTTTTAGGTTCTTTTCTATTTGGTAGAACCATTAGGGAATGGGAAAGTGCAATAGATAGGCTAAAGAATACACCGCATCCAAAAATTGTCGAGGTGCTTCAGATTAGTTTTGATGGACTACAGGAAAAAGACAAGGATATATTCCTACATATAGCTTGCTTTTACAAGGGTTGGGATAGGGACCGTGTGACACAAATACTAGACTATTGCAAGCTAGAACCTGACATTGGTTTAAGTGATCTTGCTGATAAATCTCTCATAGCCATCTCCAACAACAAACTGTCAATGCATGATTTGCTACAAGAACTGGGCAGGGAAATAGTTCGTCGACAGTCTCCTAGAGAGCCAGGCAGACGTAGTAGATTATGGTCTCATGATGACATCCACAGTGTGTTAAAGAATAATAAGGGAACAGACGCAATCCAAGCCATGGTAGTGGACTTGCCTGAGTTAGAAGTGGCTCATTGGAAACCAGAAGCCTTTTCAAATTTGTCTCAACTCAGTCTTCTCCATATTCGTAACGTGGATCTTCCCAAAGGCCTCACTTTTCTTTCTAATTCCTTGAGACTCATTGAATGGAGTGGATATCCCTTAAGATCCCTCCCACAAAATTTTGAACCAGACAAACTTATTGAACTTAACTTGTGCCACAGCAACATTGAACACCTTTGGAAAGGAGCAAAGAATTGTGACAAGTTAAAGTTCATCAAACTATGCCATTCTCAAAAAATTGTCCACACCCCGGACCTCACAGGCGTTCAAAATCTTGAGACTTTAGATCTTGAAGGATGTAAGAGTTTGGTAAAACTTCATCAATCGCTCGGACAACTCAAAAGGCTTACTGTATTGAATCTTAAAGATTGCGAGAGTCTCGAGAGTCTGCCAAGTAAGATTGAAATGGAATTTCTTGAAACTTTAATTCTTTCGAACTGCTCAAAAGTTAAAAAGATTCCTGAGTTTTCTGGAAATATGGGGCGTTTATTGGAGCTTTGTCTCGATGAGACTGCCATTGAAAAACTACCTGTGTCAATTGGATGCCTGAGTGGCCTTGCTTCGCTGAATCTGAGCAACTGCAAAAATCTTGTTTGCCTTCCAAGCACCTTTAGTAGATTGGAGTCTCTTAAAAAGCTTAATCTTTCTGGATGTTTGAAACTTGGTGAACAGGATAGCGCAAGGGAGAATGGTGCAATTAGAACTGCTGTAAATCATCTTATGAAAAATGGTTGTGAAGTTGTTGGGAAATATTTTTCATGGTCCTTGTCTTCTAGATTGGTGCAAAGAGCAGATATAGAACCAATGAGTATGCAGTCGCCTGTATCTGATCTGTGTTATTCTATGGAGCCAAATGCAAATACATCAATGAGTATCCAGTGTCTACCATCTCATCTTTGTAATTTAACAGATCTAAACATAAGTAACTGCAATCTTGGTGATGCAGCATTTTCCAACTTTGGCTGTTTTCCCTCCTTAGTGGCACTAAATCTGAGTGGAAATAATTTTGTTAAGCTTCCTCCAAGCATCAGATCCTGTTTTAAGCTTCAGAACATTAACTTGGAGAATTGCAAGACACTTCAAGAGCTGTCAGGCCTTCCATCAAATAGTAAATTGGATGTGAGGGCAGGTGGTTGTTCTTCACTGGAAATGCTGTTTGATGTATCCAATTTTAAGAGATTTGAGAAATCATATTTCAATTTCATCAATTGCTTCAAACTAAATGACAATCAGGGCTCCACTAACATAGCATTTGAAATGCTGAGGATATTCCTTAATCAGAGAATTTCTAATGCGACGGAAACTTTTCAAATTGTAATTCCTGGAAGTAGAATTCCTGAGTGGTTCGATCATCGAAGTTTTGAGAGTTCATTAATTGTAGACCTACTTCCAGGTTGGAATAATAAGAGTACGTTTTTGGGATTTGTTTTGTGTGCTGGCTTTGTACTCCATGAGTACTGTCAGGTTGATGAGCTTGATTTCCATCAGTTCAAGACTTTTAACGCTACTCATCATCTTGTATGTTGCCTGAAGCTGAATGGAAGAGAATTAAAAGTATACGGCAAACAGCCTGCATTTCGCTTTAGCAAACAACTTTGCCAAGTTGATTCAGATCACCTTTGGATATTCTATGTGTCCCGTGATAAATACTTTGGTACAGATTGGTGGCATAACAGTTGGAGTCAGCTCGAGTTCCTATTTCAAACCAGAGGGCCAGGTCTGAAGGTGAAGGAGTGCGGGTGCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1050

Amino Acids

118.56

Weight (kDa)

6.64

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 105 5.3e-15 TIR domain
NB-ARC PF00931 125 - 279 3.2e-16 NB-ARC domain
WHD_ROQ1 PF23282 346 - 416 3.3e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 545 - 803 9e-14 Leucine-rich repeat region
LRR_13 PF23286 607 - 691 5.7e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 908 - 1048 1.1e-10 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2522
Acc36I ACCTGC 1 cut(s) 2522
AccB1I GGYRCC 1 cut(s) 72
AccB7I CCANNNNNTGG 2 cut(s) 368, 1342
AccI GTMKAC 4 cut(s) 1240, 2287, 2784, 2961
AciI CCGC 5 cut(s) 70, 328, 607, 986, 3141
AclWI GGATC 7 cut(s) 50, 63, 385, 398, 1446, 1503, 2430
AcoI YGGCCR 1 cut(s) 192
AcsI RAATTY 5 cut(s) 1399, 1522, 1814, 2688, 2736
AcuI CTGAAG 4 cut(s) 995, 2435, 2954, 3146
AdeI CACNNNGTG 1 cut(s) 1303
AfaI GTAC 4 cut(s) 2815, 2850, 2861, 3061
AfiI CCNNNNNNNGG 4 cut(s) 368, 1139, 1342, 1654
AflII CTTAAG 1 cut(s) 1504
AhdI GACNNNNNGTC 1 cut(s) 29
AjnI CCWGG 5 cut(s) 549, 1258, 2725, 2794, 3118
AjuI GAANNNNNNNTTGG 4 cut(s) 1122, 1154, 2447, 2479
Alw26I GTCTC 9 cut(s) 1251, 1287, 1410, 1471, 1676, 1787, 1907, 1910, 2034
AlwI GGATC 7 cut(s) 50, 63, 385, 398, 1446, 1503, 2430
AlwNI CAGNNNCTG 1 cut(s) 2439
Ama87I CYCGRG 2 cut(s) 1784, 3092
AoxI GGCC 6 cut(s) 192, 511, 1450, 1957, 2497, 3116
ApeKI GCWGC 5 cut(s) 173, 176, 471, 776, 2348
ApoI RAATTY 5 cut(s) 1399, 1522, 1814, 2688, 2736
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
AseI ATTAAT 1 cut(s) 2777
Asp700I GAANNNNTTC 3 cut(s) 322, 1233, 2709
AspLEI GCGC 1 cut(s) 2084
AspS9I GGNCC 5 cut(s) 698, 1093, 1657, 2166, 3116
AsuC2I CCSGG 1 cut(s) 1655
AsuHPI GGTGA 5 cut(s) 347, 2081, 2354, 3011, 3142
AsuII TTCGAA 3 cut(s) 268, 857, 1838
AvaI CYCGRG 2 cut(s) 1784, 3092
AvaII GGWCC 4 cut(s) 698, 1093, 1657, 2166
BalI TGGCCA 1 cut(s) 194
BamHI GGATCC 2 cut(s) 55, 390
BanI GGYRCC 1 cut(s) 72
BanII GRGCYC 1 cut(s) 2642
BarI GAAGNNNNNNTAC 2 cut(s) 2775, 2807
BbsI GAAGAC 3 cut(s) 34, 1409, 2166
BbvCI CCTCAGC 1 cut(s) 2666
BbvI GCAGC 5 cut(s) 163, 185, 458, 763, 2360
BccI CCATC 7 cut(s) 13, 503, 1019, 1184, 2299, 2512, 2895
BceAI ACGGC 1 cut(s) 2980
BcgI CGANNNNNNTGC 4 cut(s) 837, 871, 1898, 1932
BciT130I CCWGG 5 cut(s) 551, 1260, 2727, 2796, 3120
BciVI GTATCC 3 cut(s) 557, 2288, 2578
BclI TGATCA 2 cut(s) 259, 597
BcnI CCSGG 1 cut(s) 1655
BcoDI GTCTC 9 cut(s) 1251, 1287, 1410, 1471, 1676, 1787, 1907, 1910, 2034
BfaI CTAG 5 cut(s) 515, 1112, 1127, 1251, 2178
BfmI CTRYAG 2 cut(s) 429, 1031
BfrI CTTAAG 1 cut(s) 1504
BfuAI ACCTGC 1 cut(s) 2522
BfuI GTATCC 3 cut(s) 557, 2288, 2578
BglI GCCNNNNNGGC 1 cut(s) 1956
BglII AGATCT 2 cut(s) 1690, 2314
BisI GCNGC 6 cut(s) 71, 174, 177, 472, 777, 2349
BlsI GCNGC 6 cut(s) 72, 175, 178, 473, 778, 2350
BmcAI AGTACT 1 cut(s) 2861
Bme1390I CCNGG 6 cut(s) 551, 1260, 1655, 2727, 2796, 3120
Bme18I GGWCC 4 cut(s) 698, 1093, 1657, 2166
BmeRI GACNNNNNGTC 1 cut(s) 29
BmeT110I CYCGRG 2 cut(s) 1784, 3092
BmgT120I GGNCC 5 cut(s) 698, 1093, 1657, 2166, 3116
BmiI GGNNCC 6 cut(s) 57, 74, 392, 1094, 2253, 2641
BmrFI CCNGG 6 cut(s) 551, 1260, 1655, 2727, 2796, 3120
BmrI ACTGGG 1 cut(s) 1229
BmsI GCATC 5 cut(s) 751, 997, 1937, 2335, 2439
BmuI ACTGGG 1 cut(s) 1229
BpiI GAAGAC 3 cut(s) 34, 1409, 2166
Bpu10I CCTNAGC 1 cut(s) 2666
Bpu14I TTCGAA 3 cut(s) 268, 857, 1838
BpuEI CTTGAG 3 cut(s) 873, 1495, 1700
BpuMI CCSGG 1 cut(s) 1655
BsaBI GATNNNNATC 2 cut(s) 2886, 3015
BsaI GGTCTC 1 cut(s) 1287
BsaJI CCNNGG 4 cut(s) 394, 550, 1341, 1653
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 1 cut(s) 3128
Bsc4I CCNNNNNNNGG 4 cut(s) 368, 1139, 1342, 1654
Bse1I ACTGG 4 cut(s) 695, 1224, 2281, 2553
Bse8I GATNNNNATC 2 cut(s) 2886, 3015
BseBI CCWGG 5 cut(s) 551, 1260, 2727, 2796, 3120
BseDI CCNNGG 4 cut(s) 394, 550, 1341, 1653
BseGI GGATG 8 cut(s) 514, 655, 988, 1293, 1706, 1952, 2060, 2527
BseJI GATNNNNATC 2 cut(s) 2886, 3015
BseLI CCNNNNNNNGG 4 cut(s) 368, 1139, 1342, 1654
BseMII CTCAG 8 cut(s) 1350, 1426, 1857, 1943, 1967, 2387, 2657, 2733
BseNI ACTGG 4 cut(s) 695, 1224, 2281, 2553
BseXI GCAGC 5 cut(s) 163, 185, 458, 763, 2360
BseYI CCCAGC 1 cut(s) 779
BshFI GGCC 6 cut(s) 194, 513, 1452, 1959, 2499, 3118
BshNI GGYRCC 1 cut(s) 72
BsiHKCI CYCGRG 2 cut(s) 1784, 3092
BsiSI CCGG 2 cut(s) 38, 1655
BslFI GGGAC 4 cut(s) 36, 824, 1106, 3025
BslI CCNNNNNNNGG 4 cut(s) 368, 1139, 1342, 1654
BsmAI GTCTC 9 cut(s) 1251, 1287, 1410, 1471, 1676, 1787, 1907, 1910, 2034
BsmFI GGGAC 4 cut(s) 36, 824, 1106, 3025
BsmI GAATGC 1 cut(s) 211
BsnI GGCC 6 cut(s) 194, 513, 1452, 1959, 2499, 3118
Bso31I GGTCTC 1 cut(s) 1287
BsoBI CYCGRG 2 cut(s) 1784, 3092
Bsp119I TTCGAA 3 cut(s) 268, 857, 1838
Bsp1286I GDGCHC 1 cut(s) 2642
Bsp19I CCATGG 2 cut(s) 394, 1341
BspACI CCGC 5 cut(s) 70, 328, 607, 986, 3141
BspANI GGCC 6 cut(s) 194, 513, 1452, 1959, 2499, 3118
BspCNI CTCAG 8 cut(s) 1351, 1425, 1858, 1944, 1968, 2388, 2658, 2734
BspHI TCATGA 4 cut(s) 256, 439, 793, 1285
BspLI GGNNCC 6 cut(s) 57, 74, 392, 1094, 2253, 2641
BspMI ACCTGC 1 cut(s) 2522
BspPI GGATC 7 cut(s) 50, 63, 385, 398, 1446, 1503, 2430
BspT104I TTCGAA 3 cut(s) 268, 857, 1838
BspT107I GGYRCC 1 cut(s) 72
BspTI CTTAAG 1 cut(s) 1504
BspTNI GGTCTC 1 cut(s) 1287
BsrI ACTGG 4 cut(s) 695, 1224, 2281, 2553
BssECI CCNNGG 4 cut(s) 394, 550, 1341, 1653
BssNAI GTATAC 1 cut(s) 2962
BssT1I CCWWGG 2 cut(s) 394, 1341
Bst1107I GTATAC 1 cut(s) 2962
Bst2UI CCWGG 5 cut(s) 551, 1260, 2727, 2796, 3120
Bst4CI ACNGT 8 cut(s) 366, 1097, 1194, 1245, 1301, 1756, 2864, 3080
Bst6I CTCTTC 1 cut(s) 2941
BstAFI CTTAAG 1 cut(s) 1504
BstAPI GCANNNNNTGC 1 cut(s) 1207
BstBI TTCGAA 3 cut(s) 268, 857, 1838
BstC8I GCNNGC 7 cut(s) 192, 209, 401, 1069, 1124, 2842, 2976
BstDEI CTNAG 9 cut(s) 1359, 1412, 1866, 1952, 1976, 2380, 2396, 2666, 2742
BstDSI CCRYGG 2 cut(s) 394, 1341
BstF5I GGATG 8 cut(s) 514, 655, 988, 1293, 1706, 1952, 2060, 2527
BstHHI GCGC 1 cut(s) 2084
BstMAI GTCTC 9 cut(s) 1251, 1287, 1410, 1471, 1676, 1787, 1907, 1910, 2034
BstMWI GCNNNNNNNGC 4 cut(s) 1207, 1337, 1956, 2007
BstNI CCWGG 5 cut(s) 551, 1260, 2727, 2796, 3120
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 6 cut(s) 549, 1258, 1653, 2725, 2794, 3118
BstSFI CTRYAG 2 cut(s) 429, 1031
BstV1I GCAGC 5 cut(s) 163, 185, 458, 763, 2360
BstV2I GAAGAC 3 cut(s) 34, 1409, 2166
BstX2I RGATCY 7 cut(s) 55, 390, 1438, 1508, 1690, 2314, 2435
BstXI CCANNNNNNTGG 1 cut(s) 1348
BstYI RGATCY 7 cut(s) 55, 390, 1438, 1508, 1690, 2314, 2435
BstZ17I GTATAC 1 cut(s) 2962
BsuI GTATCC 3 cut(s) 557, 2288, 2578
BsuRI GGCC 6 cut(s) 194, 513, 1452, 1959, 2499, 3118
BtgI CCRYGG 2 cut(s) 394, 1341
BtsCI GGATG 8 cut(s) 514, 655, 988, 1293, 1706, 1952, 2060, 2527
BtsIMutI CAGTG 3 cut(s) 1306, 2288, 2546
BveI ACCTGC 1 cut(s) 2522
Cac8I GCNNGC 7 cut(s) 192, 209, 401, 1069, 1124, 2842, 2976
CaiI CAGNNNCTG 1 cut(s) 2439
CciI TCATGA 4 cut(s) 256, 439, 793, 1285
CfoI GCGC 1 cut(s) 2084
Cfr13I GGNCC 5 cut(s) 698, 1093, 1657, 2166, 3116
CseI GACGC 3 cut(s) 826, 1337, 3136
Csp6I GTAC 4 cut(s) 2814, 2849, 2860, 3060
CviQI GTAC 4 cut(s) 2814, 2849, 2860, 3060
DdeI CTNAG 9 cut(s) 1359, 1412, 1866, 1952, 1976, 2380, 2396, 2666, 2742
DraI TTTAAA 1 cut(s) 819
DraIII CACNNNGTG 1 cut(s) 1303
DriI GACNNNNNGTC 1 cut(s) 29
EaeI YGGCCR 1 cut(s) 192
Eam1104I CTCTTC 1 cut(s) 2941
Eam1105I GACNNNNNGTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 2941
Eco130I CCWWGG 2 cut(s) 394, 1341
Eco147I AGGCCT 2 cut(s) 1452, 2499
Eco24I GRGCYC 1 cut(s) 2642
Eco31I GGTCTC 1 cut(s) 1287
Eco32I GATATC 1 cut(s) 1499
Eco47I GGWCC 4 cut(s) 698, 1093, 1657, 2166
Eco57I CTGAAG 4 cut(s) 995, 2435, 2954, 3146
Eco88I CYCGRG 2 cut(s) 1784, 3092
EcoRI GAATTC 1 cut(s) 2736
EcoRII CCWGG 5 cut(s) 549, 1258, 2725, 2794, 3118
EcoRV GATATC 1 cut(s) 1499
EcoT14I CCWWGG 2 cut(s) 394, 1341
EcoT22I ATGCAT 1 cut(s) 1203
EcoT38I GRGCYC 1 cut(s) 2642
ErhI CCWWGG 2 cut(s) 394, 1341
FaqI GGGAC 4 cut(s) 36, 824, 1106, 3025
FauI CCCGC 2 cut(s) 335, 3134
FbaI TGATCA 2 cut(s) 259, 597
FblI GTMKAC 4 cut(s) 1240, 2287, 2784, 2961
Fnu4HI GCNGC 6 cut(s) 71, 174, 177, 472, 777, 2349
FokI GGATG 8 cut(s) 521, 662, 975, 1280, 1713, 1959, 2067, 2534
FriOI GRGCYC 1 cut(s) 2642
Fsp4HI GCNGC 6 cut(s) 71, 174, 177, 472, 777, 2349
FspBI CTAG 5 cut(s) 515, 1112, 1127, 1251, 2178
GlaI GCGC 1 cut(s) 2083
GluI GCNGC 6 cut(s) 71, 174, 177, 472, 777, 2349
GsaI CCCAGC 1 cut(s) 783
HaeIII GGCC 6 cut(s) 194, 513, 1452, 1959, 2499, 3118
HapII CCGG 2 cut(s) 38, 1655
HgaI GACGC 3 cut(s) 826, 1337, 3136
HhaI GCGC 1 cut(s) 2084
Hin6I GCGC 1 cut(s) 2082
HinP1I GCGC 1 cut(s) 2082
HincII GTYRAC 1 cut(s) 1241
HindII GTYRAC 1 cut(s) 1241
HindIII AAGCTT 3 cut(s) 2039, 2417, 2447
HpaII CCGG 2 cut(s) 38, 1655
HphI GGTGA 5 cut(s) 347, 2081, 2354, 3011, 3142
Hpy166II GTNNAC 8 cut(s) 362, 1241, 1351, 1648, 2072, 2288, 2785, 2962
Hpy8I GTNNAC 8 cut(s) 362, 1241, 1351, 1648, 2072, 2288, 2785, 2962
Hpy99I CGWCG 2 cut(s) 1242, 2704
HpyAV CCTTC 7 cut(s) 69, 157, 1691, 2012, 2510, 3121, 3127
HpyCH4III ACNGT 8 cut(s) 366, 1097, 1194, 1245, 1301, 1756, 2864, 3080
HpyCH4IV ACGT 3 cut(s) 1267, 1434, 2816
HpyF10VI GCNNNNNNNGC 4 cut(s) 1207, 1337, 1956, 2007
HpyF3I CTNAG 9 cut(s) 1359, 1412, 1866, 1952, 1976, 2380, 2396, 2666, 2742
HpySE526I ACGT 3 cut(s) 1267, 1434, 2816
HspAI GCGC 1 cut(s) 2082
Ksp22I TGATCA 2 cut(s) 259, 597
LmnI GCTCC 6 cut(s) 99, 587, 1589, 1897, 2251, 2645
Lsp1109I GCAGC 5 cut(s) 163, 185, 458, 763, 2360
LweI GCATC 5 cut(s) 751, 997, 1937, 2335, 2439
MaeI CTAG 5 cut(s) 515, 1112, 1127, 1251, 2178
MaeII ACGT 3 cut(s) 1267, 1434, 2816
MaeIII GTNAC 6 cut(s) 686, 718, 1099, 1430, 1601, 2327
MboII GAAGA 8 cut(s) 34, 143, 282, 1409, 1433, 2166, 2535, 2958
MfeI CAATTG 2 cut(s) 1941, 2611
MflI RGATCY 7 cut(s) 55, 390, 1438, 1508, 1690, 2314, 2435
MhlI GDGCHC 1 cut(s) 2642
MlsI TGGCCA 1 cut(s) 194
MluNI TGGCCA 1 cut(s) 194
MlyI GAGTC 5 cut(s) 1473, 1789, 1798, 2036, 3094
MmeI TCCRAC 4 cut(s) 1206, 2382, 2780, 3062
Mox20I TGGCCA 1 cut(s) 194
Mph1103I ATGCAT 1 cut(s) 1203
MroXI GAANNNNTTC 3 cut(s) 322, 1233, 2709
MscI TGGCCA 1 cut(s) 194
MslI CAYNNNNRTG 1 cut(s) 1346
Msp20I TGGCCA 1 cut(s) 194
MspA1I CMGCKG 1 cut(s) 176
MspCI CTTAAG 1 cut(s) 1504
MspI CCGG 2 cut(s) 38, 1655
MspR9I CCNGG 6 cut(s) 551, 1260, 1655, 2727, 2796, 3120
MunI CAATTG 2 cut(s) 1941, 2611
Mva1269I GAATGC 1 cut(s) 211
MvaI CCWGG 5 cut(s) 551, 1260, 2727, 2796, 3120
MwoI GCNNNNNNNGC 4 cut(s) 1207, 1337, 1956, 2007
NciI CCSGG 1 cut(s) 1655
NcoI CCATGG 2 cut(s) 394, 1341
NlaIV GGNNCC 6 cut(s) 57, 74, 392, 1094, 2253, 2641
NmuCI GTSAC 3 cut(s) 686, 1099, 1601
NsiI ATGCAT 1 cut(s) 1203
NspI RCATGY 1 cut(s) 403
NspV TTCGAA 3 cut(s) 268, 857, 1838
PaeI GCATGC 1 cut(s) 403
PaeR7I CTCGAG 2 cut(s) 1784, 3092
PagI TCATGA 4 cut(s) 256, 439, 793, 1285
PaqCI CACCTGC 1 cut(s) 2522
PceI AGGCCT 2 cut(s) 1452, 2499
PctI GAATGC 1 cut(s) 211
PdmI GAANNNNTTC 3 cut(s) 322, 1233, 2709
PfeI GAWTC 6 cut(s) 287, 323, 1762, 1861, 1972, 3011
PflMI CCANNNNNTGG 2 cut(s) 368, 1342
PfoI TCCNGGA 1 cut(s) 2725
PkrI GCNGC 6 cut(s) 72, 175, 178, 473, 778, 2350
PleI GAGTC 5 cut(s) 1473, 1788, 1797, 2035, 3093
PpsI GAGTC 5 cut(s) 1473, 1788, 1797, 2035, 3093
PshBI ATTAAT 1 cut(s) 2777
Psp6I CCWGG 5 cut(s) 549, 1258, 2725, 2794, 3118
PspFI CCCAGC 1 cut(s) 779
PspGI CCWGG 5 cut(s) 549, 1258, 2725, 2794, 3118
PspN4I GGNNCC 6 cut(s) 57, 74, 392, 1094, 2253, 2641
PspPI GGNCC 5 cut(s) 698, 1093, 1657, 2166, 3116
PspXI VCTCGAGB 1 cut(s) 3092
PstNI CAGNNNCTG 1 cut(s) 2439
PsuI RGATCY 7 cut(s) 55, 390, 1438, 1508, 1690, 2314, 2435
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 4 cut(s) 2815, 2850, 2861, 3061
RsaNI GTAC 4 cut(s) 2814, 2849, 2860, 3060
RseI CAYNNNNRTG 1 cut(s) 1346
SalI GTCGAC 1 cut(s) 1239
SatI GCNGC 6 cut(s) 71, 174, 177, 472, 777, 2349
Sau96I GGNCC 5 cut(s) 698, 1093, 1657, 2166, 3116
ScaI AGTACT 1 cut(s) 2861
SchI GAGTC 5 cut(s) 1473, 1789, 1798, 2036, 3094
ScrFI CCNGG 6 cut(s) 551, 1260, 1655, 2727, 2796, 3120
SduI GDGCHC 1 cut(s) 2642
SfaNI GCATC 5 cut(s) 751, 997, 1937, 2335, 2439
SfcI CTRYAG 2 cut(s) 429, 1031
Sfr274I CTCGAG 2 cut(s) 1784, 3092
SfuI TTCGAA 3 cut(s) 268, 857, 1838
SinI GGWCC 4 cut(s) 698, 1093, 1657, 2166
SlaI CTCGAG 2 cut(s) 1784, 3092
SmiMI CAYNNNNRTG 1 cut(s) 1346
SmlI CTYRAG 6 cut(s) 888, 1474, 1504, 1679, 1784, 3092
SmoI CTYRAG 6 cut(s) 888, 1474, 1504, 1679, 1784, 3092
SphI GCATGC 1 cut(s) 403
SseBI AGGCCT 2 cut(s) 1452, 2499
SsiI CCGC 5 cut(s) 70, 328, 607, 986, 3141
SspI AATATT 2 cut(s) 562, 2156
SspMI CTAG 5 cut(s) 515, 1112, 1127, 1251, 2178
StuI AGGCCT 2 cut(s) 1452, 2499
StyD4I CCNGG 6 cut(s) 549, 1258, 1653, 2725, 2794, 3118
StyI CCWWGG 2 cut(s) 394, 1341
TaaI ACNGT 8 cut(s) 366, 1097, 1194, 1245, 1301, 1756, 2864, 3080
TaiI ACGT 3 cut(s) 1270, 1437, 2819
TaqII GACCGA 1 cut(s) 480
TatI WGTACW 2 cut(s) 2848, 2859
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 6 cut(s) 287, 323, 1762, 1861, 1972, 3011
TscAI CASTG 3 cut(s) 1306, 2288, 2553
TseFI GTSAC 3 cut(s) 686, 1099, 1601
TseI GCWGC 5 cut(s) 173, 176, 471, 776, 2348
Tsp45I GTSAC 3 cut(s) 686, 1099, 1601
TspGWI ACGGA 2 cut(s) 86, 2717
TspRI CASTG 3 cut(s) 1306, 2288, 2553
Van91I CCANNNNNTGG 2 cut(s) 368, 1342
Vha464I CTTAAG 1 cut(s) 1504
VpaK11BI GGWCC 4 cut(s) 698, 1093, 1657, 2166
VspI ATTAAT 1 cut(s) 2777
XapI RAATTY 5 cut(s) 1399, 1522, 1814, 2688, 2736
XbaI TCTAGA 1 cut(s) 2177
XceI RCATGY 1 cut(s) 403
XhoI CTCGAG 2 cut(s) 1784, 3092
XmiI GTMKAC 4 cut(s) 1240, 2287, 2784, 2961
XmnI GAANNNNTTC 3 cut(s) 322, 1233, 2709
XspI CTAG 5 cut(s) 515, 1112, 1127, 1251, 2178
ZrmI AGTACT 1 cut(s) 2861
Zsp2I ATGCAT 1 cut(s) 1203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.