Rroxscaffold_4G00327120

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Forward (+)
59548046 .. 59557610
9565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00327120.1

Sequence Viewer

Length: 1128 bp
ATGTCATCTACCCATGATCGCAGAAAATATGTGAGAGGTTCAATCTTTCATGGATCCAAAGTGGTTTGGCGATCTTTAAATAAGTTCTTGCCTTCTGGGTTGGTGCAAAAAAGGAGTTTCCGCTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTAAAGGAGCATTTGCCAACGAATTTGGTTACTTTCCTTCTTTGGTGACCTTGAATCTAAGTGGGAACAATTTTGTTCGTCTTCCTTCAGGCATTGGATTGCTTCCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTAAAGAGTTGTCGGACCTTCCATCAAATAATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATCAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTCGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAACAGGGAAACTTTTCAAATTGTAATTCCTGGAAGGAATATTCCTGAGTGGTTCAGCCATCGGAGTGTTGGGTGTTCTGTAAGTGTAGCTCTACCTGCACATTCGAAAAACAGTCGGGTTTTGGGATTTGCTCTGTGTGCTGTTTTTGTAGTCCAAGAGCACCATCAGATGGATAAGCCTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCGATGGAAGAGAATTGGAAGTGTATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCAAGGTTAAGTCGGATCACCTGTGGCTATTCTATGTATCTGGTGATAAATACTTTGGTACAAAGTGGTGGCATAACAGTTACAGTCAGTTTGAGTTCTTATTTGAAACCAGAGGGCCAGGTCTGCAGGTGAAGGATTGTGGAGTCCGTCTGATATATGAGCGAGATGTGCAAGAGTTGAGCCGGACAACCACTCAATCAAGCAGTAGGGTGTCTCCTTATGAGGATATATTGATTGATTTTGACATTCCGGTTGAAGGGGAAACCAGTGGCACTGGTAGCAGAACTTGCACGCTTGAAGAATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

375

Amino Acids

42.8

Weight (kDa)

8.71

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C-JID PF20160 182 - 331 8.5e-19 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 937
Acc36I ACCTGC 2 cut(s) 622, 937
AccB7I CCANNNNNTGG 1 cut(s) 688
AciI CCGC 1 cut(s) 121
AclWI GGATC 3 cut(s) 48, 61, 843
AcsI RAATTY 4 cut(s) 203, 394, 710, 815
AcuI CTGAAG 3 cut(s) 252, 509, 773
AfaI GTAC 2 cut(s) 364, 880
AfiI CCNNNNNNNGG 3 cut(s) 134, 688, 1076
AjnI CCWGG 2 cut(s) 547, 937
AjuI GAANNNNNNNTTGG 2 cut(s) 290, 322
AluBI AGCT 3 cut(s) 292, 608, 757
AluI AGCT 3 cut(s) 292, 608, 757
Alw21I GWGCWC 1 cut(s) 681
Alw26I GTCTC 2 cut(s) 316, 1038
AlwI GGATC 3 cut(s) 48, 61, 843
AoxI GGCC 1 cut(s) 935
ApoI RAATTY 4 cut(s) 203, 394, 710, 815
Asp700I GAANNNNTTC 2 cut(s) 494, 531
AspS9I GGNCC 2 cut(s) 340, 935
AsuHPI GGTGA 4 cut(s) 238, 830, 875, 961
AsuII TTCGAA 2 cut(s) 480, 623
AvaII GGWCC 1 cut(s) 340
BamHI GGATCC 1 cut(s) 53
BbsI GAAGAC 2 cut(s) 254, 497
Bbv12I GWGCWC 1 cut(s) 681
BccI CCATC 5 cut(s) 355, 585, 682, 690, 755
BciT130I CCWGG 2 cut(s) 549, 939
BcoDI GTCTC 2 cut(s) 316, 1038
BfmI CTRYAG 1 cut(s) 944
BfuAI ACCTGC 2 cut(s) 622, 937
Bme1390I CCNGG 2 cut(s) 549, 939
Bme18I GGWCC 1 cut(s) 340
BmgT120I GGNCC 2 cut(s) 340, 935
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 2 cut(s) 549, 939
BmsI GCATC 3 cut(s) 376, 398, 452
BpiI GAAGAC 2 cut(s) 254, 497
Bpu10I CCTNAGC 1 cut(s) 288
Bpu14I TTCGAA 2 cut(s) 480, 623
BpuEI CTTGAG 1 cut(s) 314
BsaWI WCCGGW 1 cut(s) 1069
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 688, 1076
Bse1I ACTGG 2 cut(s) 1086, 1099
BseBI CCWGG 2 cut(s) 549, 939
BseGI GGATG 1 cut(s) 467
BseLI CCNNNNNNNGG 3 cut(s) 134, 688, 1076
BseMII CTCAG 2 cut(s) 155, 555
BseNI ACTGG 2 cut(s) 1086, 1099
BsgI GTGCAG 1 cut(s) 600
BshFI GGCC 1 cut(s) 937
BsiHKAI GWGCWC 1 cut(s) 681
BsiSI CCGG 2 cut(s) 1003, 1070
BslI CCNNNNNNNGG 3 cut(s) 134, 688, 1076
BsmAI GTCTC 2 cut(s) 316, 1038
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 1 cut(s) 937
Bsp119I TTCGAA 2 cut(s) 480, 623
Bsp1286I GDGCHC 1 cut(s) 681
Bsp143I GATC 5 cut(s) 16, 53, 71, 404, 835
BspACI CCGC 1 cut(s) 121
BspANI GGCC 1 cut(s) 937
BspCNI CTCAG 2 cut(s) 156, 556
BspLI GGNNCC 1 cut(s) 55
BspMAI CTGCAG 1 cut(s) 948
BspMI ACCTGC 2 cut(s) 622, 937
BspPI GGATC 3 cut(s) 48, 61, 843
BspT104I TTCGAA 2 cut(s) 480, 623
BsrI ACTGG 2 cut(s) 1086, 1099
BssMI GATC 5 cut(s) 16, 53, 71, 404, 835
Bst2UI CCWGG 2 cut(s) 549, 939
Bst4CI ACNGT 3 cut(s) 632, 899, 905
Bst6I CTCTTC 1 cut(s) 760
BstAPI GCANNNNNTGC 1 cut(s) 1107
BstBI TTCGAA 2 cut(s) 480, 623
BstC8I GCNNGC 3 cut(s) 125, 795, 1112
BstDEI CTNAG 4 cut(s) 164, 239, 288, 564
BstEII GGTNACC 1 cut(s) 226
BstF5I GGATG 1 cut(s) 467
BstKTI GATC 5 cut(s) 19, 56, 74, 407, 838
BstMAI GTCTC 2 cut(s) 316, 1038
BstMBI GATC 5 cut(s) 16, 53, 71, 404, 835
BstMWI GCNNNNNNNGC 7 cut(s) 289, 614, 656, 943, 988, 1098, 1107
BstNI CCWGG 2 cut(s) 549, 939
BstPI GGTNACC 1 cut(s) 226
BstSCI CCNGG 2 cut(s) 547, 937
BstSFI CTRYAG 1 cut(s) 944
BstV2I GAAGAC 2 cut(s) 254, 497
BstX2I RGATCY 1 cut(s) 53
BstYI RGATCY 1 cut(s) 53
BsuRI GGCC 1 cut(s) 937
BtsCI GGATG 1 cut(s) 467
BtsIMutI CAGTG 3 cut(s) 368, 1092, 1093
BveI ACCTGC 2 cut(s) 622, 937
Cac8I GCNNGC 3 cut(s) 125, 795, 1112
Cfr13I GGNCC 2 cut(s) 340, 935
Csp6I GTAC 2 cut(s) 363, 879
CviAII CATG 3 cut(s) 14, 50, 499
CviJI RGCY 9 cut(s) 292, 576, 608, 697, 757, 793, 847, 937, 1002
CviKI_1 RGCY 9 cut(s) 292, 576, 608, 697, 757, 793, 847, 937, 1002
CviQI GTAC 2 cut(s) 363, 879
DdeI CTNAG 4 cut(s) 164, 239, 288, 564
DpnI GATC 5 cut(s) 18, 55, 73, 406, 837
DpnII GATC 5 cut(s) 16, 53, 71, 404, 835
DraI TTTAAA 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 760
EarI CTCTTC 1 cut(s) 760
Eco47I GGWCC 1 cut(s) 340
Eco57I CTGAAG 3 cut(s) 252, 509, 773
Eco91I GGTNACC 1 cut(s) 226
EcoO65I GGTNACC 1 cut(s) 226
EcoRI GAATTC 1 cut(s) 710
EcoRII CCWGG 2 cut(s) 547, 937
EcoT22I ATGCAT 1 cut(s) 391
FaeI CATG 3 cut(s) 17, 53, 502
FatI CATG 3 cut(s) 13, 49, 498
FokI GGATG 1 cut(s) 474
HaeIII GGCC 1 cut(s) 937
HapII CCGG 2 cut(s) 1003, 1070
Hin1II CATG 3 cut(s) 17, 53, 502
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 3 cut(s) 235, 510, 963
HpaII CCGG 2 cut(s) 1003, 1070
HphI GGTGA 4 cut(s) 238, 830, 875, 961
Hpy188I TCNGA 6 cut(s) 159, 340, 582, 687, 835, 972
Hpy188III TCNNGA 2 cut(s) 563, 715
HpyAV CCTTC 7 cut(s) 102, 228, 276, 353, 546, 946, 1070
HpyCH4III ACNGT 3 cut(s) 632, 899, 905
HpyF10VI GCNNNNNNNGC 7 cut(s) 289, 614, 656, 943, 988, 1098, 1107
HpyF3I CTNAG 4 cut(s) 164, 239, 288, 564
Hsp92II CATG 3 cut(s) 17, 53, 502
Kzo9I GATC 5 cut(s) 16, 53, 71, 404, 835
LmnI GCTCC 1 cut(s) 188
LweI GCATC 3 cut(s) 376, 398, 452
MaeIII GTNAC 3 cut(s) 209, 226, 899
MalI GATC 5 cut(s) 18, 55, 73, 406, 837
MboI GATC 5 cut(s) 16, 53, 71, 404, 835
MboII GAAGA 4 cut(s) 254, 502, 777, 824
MfeI CAATTG 1 cut(s) 433
MflI RGATCY 1 cut(s) 53
MhlI GDGCHC 1 cut(s) 681
MlyI GAGTC 1 cut(s) 972
MmeI TCCRAC 2 cut(s) 318, 813
MnlI CCTC 3 cut(s) 29, 926, 1036
Mph1103I ATGCAT 1 cut(s) 391
MroXI GAANNNNTTC 2 cut(s) 494, 531
MseI TTAA 6 cut(s) 77, 149, 303, 327, 723, 828
MslI CAYNNNNRTG 1 cut(s) 582
MspI CCGG 2 cut(s) 1003, 1070
MspR9I CCNGG 2 cut(s) 549, 939
MunI CAATTG 1 cut(s) 433
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 2 cut(s) 549, 939
MwoI GCNNNNNNNGC 7 cut(s) 289, 614, 656, 943, 988, 1098, 1107
NdeII GATC 5 cut(s) 16, 53, 71, 404, 835
NlaIII CATG 3 cut(s) 17, 53, 502
NlaIV GGNNCC 1 cut(s) 55
NmuCI GTSAC 1 cut(s) 226
NsiI ATGCAT 1 cut(s) 391
NspV TTCGAA 2 cut(s) 480, 623
PaqCI CACCTGC 1 cut(s) 937
PctI GAATGC 1 cut(s) 476
PdmI GAANNNNTTC 2 cut(s) 494, 531
PfeI GAWTC 2 cut(s) 235, 510
PflMI CCANNNNNTGG 1 cut(s) 688
PfoI TCCNGGA 1 cut(s) 547
PleI GAGTC 1 cut(s) 971
PpsI GAGTC 1 cut(s) 971
Psp6I CCWGG 2 cut(s) 547, 937
PspEI GGTNACC 1 cut(s) 226
PspGI CCWGG 2 cut(s) 547, 937
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 2 cut(s) 340, 935
PstI CTGCAG 1 cut(s) 948
PsuI RGATCY 1 cut(s) 53
RsaI GTAC 2 cut(s) 364, 880
RsaNI GTAC 2 cut(s) 363, 879
RseI CAYNNNNRTG 1 cut(s) 582
SaqAI TTAA 6 cut(s) 77, 149, 303, 327, 723, 828
Sau3AI GATC 5 cut(s) 16, 53, 71, 404, 835
Sau96I GGNCC 2 cut(s) 340, 935
SchI GAGTC 1 cut(s) 972
ScrFI CCNGG 2 cut(s) 549, 939
SduI GDGCHC 1 cut(s) 681
SfaNI GCATC 3 cut(s) 376, 398, 452
SfcI CTRYAG 1 cut(s) 944
SfuI TTCGAA 2 cut(s) 480, 623
SinI GGWCC 1 cut(s) 340
SmiMI CAYNNNNRTG 1 cut(s) 582
SmlI CTYRAG 1 cut(s) 293
SmoI CTYRAG 1 cut(s) 293
SsiI CCGC 1 cut(s) 121
SspI AATATT 1 cut(s) 559
StyD4I CCNGG 2 cut(s) 547, 937
TaaI ACNGT 3 cut(s) 632, 899, 905
TaqI TCGA 3 cut(s) 480, 623, 759
TatI WGTACW 1 cut(s) 362
TfiI GAWTC 2 cut(s) 235, 510
Tru1I TTAA 6 cut(s) 77, 149, 303, 327, 723, 828
Tru9I TTAA 6 cut(s) 77, 149, 303, 327, 723, 828
TscAI CASTG 3 cut(s) 375, 1093, 1099
TseFI GTSAC 1 cut(s) 226
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 4 cut(s) 38, 418, 487, 723
TspGWI ACGGA 1 cut(s) 956
TspRI CASTG 3 cut(s) 375, 1093, 1099
Van91I CCANNNNNTGG 1 cut(s) 688
VpaK11BI GGWCC 1 cut(s) 340
XapI RAATTY 4 cut(s) 203, 394, 710, 815
XcmI CCANNNNNNNNNTGG 1 cut(s) 584
XmnI GAANNNNTTC 2 cut(s) 494, 531
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.