RchiOBHm_Chr6g0250801

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000105
Physical Location & Seq
Forward (+)
6156839 .. 6158778
1940 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22481

Sequence Viewer

Length: 993 bp
ATGAGCAATATACCATCCTCTATTTCCCGTTTGGAAAATCTTGAAGTGTTATCTCTTTGTGGATGTAAAGCAATGTCAGGCAACATGACAAGATATCCCCTCAGTTTTCTGTTGCCCATAAGTGGTTCAGGTGGTTTGTGTTCTTTAACGGAGCTAAATCTCAGTGACTGTAATCTTCAGGAGGGAAGCATACTGGAAGATTTTGGCTGCTTATACCCTTTAGTTTCACTGAATCTAAGCAAAAACAACTGTGATAGTCTTCCAAAAAGCATCAAACAGCTTTCAAAGCTTCGCAACTTGAATTTGGAAAGTTGTAAGACTCTTCAAAAATTGCCAGACCTCTCATCAAGCTTAAACTTCAGTTTCAGGGCAGATGGCTGTTTTTCACGGGAAAGGCTGTCATCGTGTTTCAATCTGATTAATTGCTCCAAATTGGTTGTCAATCAACGATGCAACAATATAGCATTGATAATGCTATGGAGATTTCTTCAGGGAATTGCTTCTGCAGGAAATAGATTTGAAACTATAATTCCTGGCAGTGAAATCTCTGAGCTGTTCAGTGATCAAAGTGTGGGACCAGTTATAAGCATGGAGTTACATCAACAATGGCAGAAGAATAAGTGGATCGGATATGTATTATGTGCTGTTTTTGTTATCAGTCGAGATCTTCCAGTTTCAGCTAACCGGCTTGGTAAATGGAGGTTCGGGACCCATAGTACTGCTCATGGTCTTCGATGCGAAGTGAAGCCCGAGAAGCTGGGTGTGACTGGCTGGTGTCCTTTCTTTGCCTGCAGCCAAGAATTAGGCCAAATTGAGTCGGAACACCTTTGGCTATCCTTTGTCTCTGGTGATTATTTTGGAACAGCATGGCAAGAAACCTGTCATAAGATCGAGTTTACATTTAGAACATTAGGCACTGGTATGGAGGTGAAGAAGTGTGGAGTCTGTTTGATATACGAGCAAGATTTAGATTTGATTGGAATGAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

330

Amino Acids

36.85

Weight (kDa)

7.8

Isoelectric Point (pI)

48.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 51 - 113 2.8e-06 Leucine-rich repeat region
C-JID PF20160 177 - 323 2.4e-09 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 584
AclWI GGATC 1 cut(s) 632
AcsI RAATTY 1 cut(s) 301
AcuI CTGAAG 3 cut(s) 161, 343, 473
AfaI GTAC 1 cut(s) 718
AfiI CCNNNNNNNGG 1 cut(s) 122
AgsI TTSAA 6 cut(s) 44, 285, 301, 326, 412, 521
AjnI CCWGG 1 cut(s) 532
AluBI AGCT 7 cut(s) 154, 280, 289, 351, 553, 680, 757
AluI AGCT 7 cut(s) 154, 280, 289, 351, 553, 680, 757
Alw26I GTCTC 1 cut(s) 847
AlwI GGATC 1 cut(s) 632
AlwNI CAGNNNCTG 1 cut(s) 168
Ama87I CYCGRG 1 cut(s) 749
AoxI GGCC 1 cut(s) 805
ApeKI GCWGC 2 cut(s) 207, 792
ApoI RAATTY 1 cut(s) 301
ArsI GACNNNNNNTTYG 2 cut(s) 423, 455
AseI ATTAAT 1 cut(s) 420
Asp700I GAANNNNTTC 1 cut(s) 499
AspS9I GGNCC 2 cut(s) 575, 708
AsuHPI GGTGA 2 cut(s) 860, 940
AvaI CYCGRG 1 cut(s) 749
AvaII GGWCC 2 cut(s) 575, 708
BbsI GAAGAC 2 cut(s) 251, 722
BbvI GCAGC 2 cut(s) 194, 804
BccI CCATC 2 cut(s) 22, 368
BciT130I CCWGG 1 cut(s) 534
BclI TGATCA 1 cut(s) 562
BcoDI GTCTC 1 cut(s) 847
BfmI CTRYAG 2 cut(s) 504, 790
BglII AGATCT 1 cut(s) 664
BisI GCNGC 2 cut(s) 208, 793
BlsI GCNGC 2 cut(s) 209, 794
BmcAI AGTACT 1 cut(s) 718
Bme1390I CCNGG 1 cut(s) 534
Bme18I GGWCC 2 cut(s) 575, 708
BmeT110I CYCGRG 1 cut(s) 749
BmgT120I GGNCC 2 cut(s) 575, 708
BmiI GGNNCC 3 cut(s) 576, 709, 710
BmrFI CCNGG 1 cut(s) 534
BmsI GCATC 3 cut(s) 279, 440, 725
BpiI GAAGAC 2 cut(s) 251, 722
Bsc4I CCNNNNNNNGG 1 cut(s) 122
Bse118I RCCGGY 1 cut(s) 684
Bse1I ACTGG 5 cut(s) 198, 578, 671, 772, 922
Bse3DI GCAATG 1 cut(s) 78
BseBI CCWGG 1 cut(s) 534
BseGI GGATG 2 cut(s) 14, 68
BseLI CCNNNNNNNGG 1 cut(s) 122
BseMI GCAATG 1 cut(s) 78
BseMII CTCAG 3 cut(s) 115, 175, 540
BseNI ACTGG 5 cut(s) 198, 578, 671, 772, 922
BseXI GCAGC 2 cut(s) 194, 804
BseYI CCCAGC 1 cut(s) 757
BshFI GGCC 1 cut(s) 807
BsiHKCI CYCGRG 1 cut(s) 749
BsiSI CCGG 1 cut(s) 685
BslFI GGGAC 2 cut(s) 588, 721
BslI CCNNNNNNNGG 1 cut(s) 122
BsmAI GTCTC 1 cut(s) 847
BsmFI GGGAC 2 cut(s) 588, 721
BsnI GGCC 1 cut(s) 807
BsoBI CYCGRG 1 cut(s) 749
Bsp143I GATC 4 cut(s) 562, 624, 664, 888
BspANI GGCC 1 cut(s) 807
BspCNI CTCAG 3 cut(s) 114, 174, 541
BspLI GGNNCC 3 cut(s) 576, 709, 710
BspMAI CTGCAG 2 cut(s) 508, 794
BspPI GGATC 1 cut(s) 632
BsrDI GCAATG 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 684
BsrI ACTGG 5 cut(s) 198, 578, 671, 772, 922
BssAI RCCGGY 1 cut(s) 684
BssMI GATC 4 cut(s) 562, 624, 664, 888
Bst2UI CCWGG 1 cut(s) 534
Bst4CI ACNGT 2 cut(s) 170, 251
Bst6I CTCTTC 1 cut(s) 327
BstC8I GCNNGC 1 cut(s) 790
BstDEI CTNAG 4 cut(s) 101, 161, 236, 549
BstF5I GGATG 2 cut(s) 14, 68
BstKTI GATC 4 cut(s) 565, 627, 667, 891
BstMAI GTCTC 1 cut(s) 847
BstMBI GATC 4 cut(s) 562, 624, 664, 888
BstMWI GCNNNNNNNGC 2 cut(s) 286, 754
BstNI CCWGG 1 cut(s) 534
BstSCI CCNGG 1 cut(s) 532
BstSFI CTRYAG 2 cut(s) 504, 790
BstV1I GCAGC 2 cut(s) 194, 804
BstV2I GAAGAC 2 cut(s) 251, 722
BstX2I RGATCY 1 cut(s) 664
BstYI RGATCY 1 cut(s) 664
BsuRI GGCC 1 cut(s) 807
BtsCI GGATG 2 cut(s) 14, 68
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 5 cut(s) 169, 227, 544, 565, 915
Cac8I GCNNGC 1 cut(s) 790
CaiI CAGNNNCTG 1 cut(s) 168
Cfr10I RCCGGY 1 cut(s) 684
Cfr13I GGNCC 2 cut(s) 575, 708
Csp6I GTAC 1 cut(s) 717
CviAII CATG 4 cut(s) 85, 589, 725, 867
CviQI GTAC 1 cut(s) 717
DdeI CTNAG 4 cut(s) 101, 161, 236, 549
DpnI GATC 4 cut(s) 564, 626, 666, 890
DpnII GATC 4 cut(s) 562, 624, 664, 888
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Eco32I GATATC 1 cut(s) 95
Eco47I GGWCC 2 cut(s) 575, 708
Eco57I CTGAAG 3 cut(s) 161, 343, 473
Eco88I CYCGRG 1 cut(s) 749
EcoO109I RGGNCCY 1 cut(s) 708
EcoRII CCWGG 1 cut(s) 532
EcoRV GATATC 1 cut(s) 95
FaeI CATG 4 cut(s) 88, 592, 728, 870
FaqI GGGAC 2 cut(s) 588, 721
FatI CATG 4 cut(s) 84, 588, 724, 866
FbaI TGATCA 1 cut(s) 562
Fnu4HI GCNGC 2 cut(s) 208, 793
FokI GGATG 1 cut(s) 75
Fsp4HI GCNGC 2 cut(s) 208, 793
GluI GCNGC 2 cut(s) 208, 793
GsaI CCCAGC 1 cut(s) 761
HaeIII GGCC 1 cut(s) 807
HapII CCGG 1 cut(s) 685
Hin1II CATG 4 cut(s) 88, 592, 728, 870
HindIII AAGCTT 2 cut(s) 287, 349
HinfI GANTC 4 cut(s) 232, 319, 815, 942
HpaII CCGG 1 cut(s) 685
HphI GGTGA 2 cut(s) 860, 940
Hpy166II GTNNAC 1 cut(s) 897
Hpy188I TCNGA 4 cut(s) 417, 550, 629, 820
Hpy188III TCNNGA 4 cut(s) 41, 179, 662, 706
Hpy8I GTNNAC 1 cut(s) 897
HpyCH4III ACNGT 2 cut(s) 170, 251
HpyCH4V TGCA 3 cut(s) 453, 506, 792
HpyF10VI GCNNNNNNNGC 2 cut(s) 286, 754
HpyF3I CTNAG 4 cut(s) 101, 161, 236, 549
Hsp92II CATG 4 cut(s) 88, 592, 728, 870
KflI GGGWCCC 1 cut(s) 708
Ksp22I TGATCA 1 cut(s) 562
Kzo9I GATC 4 cut(s) 562, 624, 664, 888
LmnI GCTCC 2 cut(s) 151, 431
Lsp1109I GCAGC 2 cut(s) 194, 804
LweI GCATC 3 cut(s) 279, 440, 725
MaeIII GTNAC 3 cut(s) 164, 594, 763
MalI GATC 4 cut(s) 564, 626, 666, 890
MboI GATC 4 cut(s) 562, 624, 664, 888
MboII GAAGA 9 cut(s) 167, 209, 251, 314, 479, 625, 659, 722, 943
MflI RGATCY 1 cut(s) 664
MluCI AATT 8 cut(s) 301, 329, 421, 431, 495, 528, 800, 810
MlyI GAGTC 3 cut(s) 313, 824, 951
MmeI TCCRAC 1 cut(s) 798
MnlI CCTC 6 cut(s) 28, 110, 175, 350, 693, 919
MroXI GAANNNNTTC 1 cut(s) 499
MseI TTAA 3 cut(s) 146, 353, 420
MslI CAYNNNNRTG 1 cut(s) 920
MspI CCGG 1 cut(s) 685
MspR9I CCNGG 1 cut(s) 534
MvaI CCWGG 1 cut(s) 534
MwoI GCNNNNNNNGC 2 cut(s) 286, 754
NdeII GATC 4 cut(s) 562, 624, 664, 888
NlaIII CATG 4 cut(s) 88, 592, 728, 870
NlaIV GGNNCC 3 cut(s) 576, 709, 710
NmuCI GTSAC 2 cut(s) 164, 763
PdmI GAANNNNTTC 1 cut(s) 499
PfeI GAWTC 1 cut(s) 232
PkrI GCNGC 2 cut(s) 209, 794
PleI GAGTC 3 cut(s) 313, 823, 950
PpsI GAGTC 3 cut(s) 313, 823, 950
PpuMI RGGWCCY 1 cut(s) 708
PshBI ATTAAT 1 cut(s) 420
PsiI TTATAA 1 cut(s) 584
Psp5II RGGWCCY 1 cut(s) 708
Psp6I CCWGG 1 cut(s) 532
PspFI CCCAGC 1 cut(s) 757
PspGI CCWGG 1 cut(s) 532
PspN4I GGNNCC 3 cut(s) 576, 709, 710
PspPI GGNCC 2 cut(s) 575, 708
PspPPI RGGWCCY 1 cut(s) 708
PstI CTGCAG 2 cut(s) 508, 794
PstNI CAGNNNCTG 1 cut(s) 168
PsuI RGATCY 1 cut(s) 664
RsaI GTAC 1 cut(s) 718
RsaNI GTAC 1 cut(s) 717
RseI CAYNNNNRTG 1 cut(s) 920
SaqAI TTAA 3 cut(s) 146, 353, 420
SatI GCNGC 2 cut(s) 208, 793
Sau3AI GATC 4 cut(s) 562, 624, 664, 888
Sau96I GGNCC 2 cut(s) 575, 708
ScaI AGTACT 1 cut(s) 718
SchI GAGTC 3 cut(s) 313, 824, 951
ScrFI CCNGG 1 cut(s) 534
SfaNI GCATC 3 cut(s) 279, 440, 725
SfcI CTRYAG 2 cut(s) 504, 790
SinI GGWCC 2 cut(s) 575, 708
SmiMI CAYNNNNRTG 1 cut(s) 920
Sse9I AATT 8 cut(s) 301, 329, 421, 431, 495, 528, 800, 810
StyD4I CCNGG 1 cut(s) 532
TaaI ACNGT 2 cut(s) 170, 251
TaqI TCGA 3 cut(s) 661, 733, 891
TasI AATT 8 cut(s) 301, 329, 421, 431, 495, 528, 800, 810
TatI WGTACW 1 cut(s) 716
TfiI GAWTC 1 cut(s) 232
Tru1I TTAA 3 cut(s) 146, 353, 420
Tru9I TTAA 3 cut(s) 146, 353, 420
TscAI CASTG 5 cut(s) 169, 234, 544, 565, 922
TseFI GTSAC 2 cut(s) 164, 763
TseI GCWGC 2 cut(s) 207, 792
Tsp45I GTSAC 2 cut(s) 164, 763
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 5 cut(s) 169, 234, 544, 565, 922
VpaK11BI GGWCC 2 cut(s) 575, 708
VspI ATTAAT 1 cut(s) 420
XapI RAATTY 1 cut(s) 301
XmnI GAANNNNTTC 1 cut(s) 499
ZrmI AGTACT 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.