Rroxscaffold_4G00327230

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Forward (+)
59761966 .. 59767063
5098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00327230.1

Sequence Viewer

Length: 3561 bp
ATGGAAATACGATGTGTTTCTAAGTTTCAGGGGGAAGATACGCGCGGGGGTTTTACCAGTTATCTGTATGGTAAACTAGATCAGAAAGGAATTCTCACCTTTATGGATGACCGAGAGCTCGAGAAAGGAAAATCCATTAGACCTGAACTTTTAGCTGCAATTGAGGAGTCTAGATGTGCGATTGTCATTCTCTCACGGAGATATACTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGCTGGGCCAACAAGTCCTCCCCGTCTTCTTAGGCGTGGATCCTTCTGATGTGCGGCACCAAAGGGGAAGGTTTCAGCTCAAAAGGGAAACCCAAATAGATGTGGAAGTAAGGGAACATGAAGAAGTTTATGGGAAGAAGAATGAGGACAGACTAGATGCGTGGAGAGCTGCTTTGACTGAGGTCGCCAATCTTTCTGGCTGGCATGTCAGGACTTATCCATATAATACAGAAGTAATTGATGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTTCCGCATTCATGATTTATTAAGCAATTACATACGTCCACAGCTTGGTCGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACACTTGCTCGAGCTATCCATGATCAAATTTGTCAGGATTTTGACCGATCCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAACCAATGGCCTTGTTTCTCTACAAGAAAAACTTCTTTCCAAAATCCTGATGGGAAAAATTGAAAACATAGAGGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGCAGTCGCAACTGGTTTGGTCCAGGGAGTAGAATTCTCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGCTAACGGGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAGGGCTTTTAAGAAATGTCCCCCACCAGAAGATTATTTGCATCTGTGCTACCATATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTCTTTGGTAGAAGCGCTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCACATAAACATATTATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAAAGAAATATTCCTACATATCGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATAGATCTCTCATTACTATCTCCAACAACGAACTGTCGATGCATGATTTGCTACAAGAAACAGGCTGGGAAATTGTTCATGAACAGTCTCCTACAGATCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGGAGAGAAATAAGGGAACAGATTCAATCCAAGGAATGGTAATGGAGTTGACTAAATTAAAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTCGTAATGTGGACCTTCCCAAAGGCCTCACATGTCTTTCTAATTCCTTGAGACTCCTTGAATGGACTGGGTGTCCCTTAAGATCTCTCCCCAAAAAATTTAAAGCAAATGAACTTCATGAACTTAACTTGTGCCACAGCAACATTAAACAGCTTTGGAAGGGAACAAAGAATTTCGACAAGTTGAAGTTCATCAAACTCCGCCATTCTCAAACCATTGTGGAGACCCCAGACCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCATTTGGTAAGAATCCATCAATCCCTTGGATTTCACAAAAAGCTTATTTTCCTGAATCTTAAAGACTGCAAAAGTCTCAGGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAGCTGCTCAAAAGTTGAGAAGATTCCCGAGTTTGTTGGAAATATGGAATGCTTGTCGGTGCTCTATCTTGATGAGACTGCCATTAAGGAACTGCCTGTTTCAATTGAACGGCTGAGTGGCCTTGTGTCATTGAATCTAAGCAATTGCAAAAATCTTGTTTTTCTCCCAAGCACCATCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTAAATGTGGGGGAGATGGATTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATAAAAATGTCATCTACCCATGATCACATAAAATATTTGAGAGGTTCAATCTTTCATGGATGCAAAGTAGTTTGGGGATCTTTAAATAAGTTCTTGCCTTCTGGATTGTTGCAAAAAGTGAATATAGAGCCAATGAGTTTCCGCTTGCCTATTTCTGGTCTGTGTAATTTAACGTATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAATGAATTCGGTTACTTTCCCTCTTTGGTTACCTTGAATCTAAGTGGAAACAATTTTGTTCGCGTTCCTTCAGGCATTCGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGACTAGACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAACCAAGGATGCGATAACATAGCATTTGAAATGCTGAAGGCATTCATGTATCAGGGAATCTGTAATAAGAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCATGAGTGGTTCAGCTATCAGAGTGTTGGGTGTTCATTAAGTGTATCTCTACCTGCAAATTGGAATAAGAGTCGGTTTTTGGGATTTGCTTTGTGTGTTGTTTTTGTACTCCATGAGCACCATCCTGTGGATGAGCTTTATATTGATGAATTCAAGACTTTTAATGCAACGCATCATCTTGTATGTTGCCTGAAGCTCGATGGAAGAGAATTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTGAATCAGATCACCTGTGGCTATTCTATGTATCTTGTGATAAATACTTTGGTACAGAGTGGTGGCATAATAGTTGCAGTCATCTTGAGTTCTTATTTGAAACCAGAGGGCTGGGTCTGAATGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAAGATATGCAAGAGTTGAACCAAACAACCACTCAATCAAGCAGTGGGATGTCTCCTTATGAGGAGATATTGATTGGTTTTGACATTCCAGTTGCAGGGGAAACCAGTGGCACTGGTAGCAGAACTTGCACGCTTGAAGAGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1186

Amino Acids

134.77

Weight (kDa)

6.36

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 7 - 183 1.6e-46 TIR domain
TIR_2 PF13676 17 - 107 2.1e-12 TIR domain
NB-ARC PF00931 210 - 363 4.2e-18 NB-ARC domain
AAA_22 PF13401 211 - 310 9.5e-06 AAA domain
NACHT PF05729 213 - 314 1.7e-06 NACHT domain
WHD_ROQ1 PF23282 430 - 501 2.3e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 690 - 775 1.4e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 701 - 770 8.2e-10 Leucine-rich repeat region
C-JID PF20160 993 - 1141 4.2e-18 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1910
Acc36I ACCTGC 2 cut(s) 1910, 3055
AccB1I GGYRCC 1 cut(s) 315
AccB7I CCANNNNNTGG 3 cut(s) 620, 1597, 3121
AccI GTMKAC 2 cut(s) 536, 2770
AccII CGCG 3 cut(s) 43, 45, 2673
AciI CCGC 7 cut(s) 45, 313, 580, 655, 862, 1882, 2533
AclWI GGATC 9 cut(s) 293, 306, 637, 650, 714, 912, 1356, 1505, 2476
AcuI CTGAAG 3 cut(s) 2664, 2942, 3206
AfaI GTAC 3 cut(s) 2797, 3102, 3313
AfeI AGCGCT 1 cut(s) 1198
AfiI CCNNNNNNNGG 6 cut(s) 620, 1394, 1597, 2546, 3121, 3509
AflII CTTAAG 1 cut(s) 1759
AflIII ACRYGT 1 cut(s) 1712
AhdI GACNNNNNGTC 1 cut(s) 1752
AjnI CCWGG 4 cut(s) 955, 1513, 2980, 3255
AjuI GAANNNNNNNTTGG 2 cut(s) 2702, 2734
Alw21I GWGCWC 3 cut(s) 120, 2157, 3114
AlwI GGATC 9 cut(s) 293, 306, 637, 650, 714, 912, 1356, 1505, 2476
AlwNI CAGNNNCTG 1 cut(s) 845
Ama87I CYCGRG 3 cut(s) 119, 681, 2120
Aor51HI AGCGCT 1 cut(s) 1198
AoxI GGCC 4 cut(s) 265, 763, 1705, 2212
ApeKI GCWGC 4 cut(s) 155, 428, 845, 2097
AseI ATTAAT 1 cut(s) 2084
Asp700I GAANNNNTTC 6 cut(s) 786, 1056, 1488, 1582, 2927, 2964
AspLEI GCGC 2 cut(s) 45, 1199
AspS9I GGNCC 3 cut(s) 265, 953, 1693
AsuHPI GGTGA 3 cut(s) 88, 2606, 3263
AvaI CYCGRG 3 cut(s) 119, 681, 2120
AvaII GGWCC 2 cut(s) 953, 1693
BamHI GGATCC 2 cut(s) 298, 642
BanI GGYRCC 1 cut(s) 315
BanII GRGCYC 1 cut(s) 120
BauI CACGAG 1 cut(s) 1163
BbsI GAAGAC 3 cut(s) 277, 1551, 1664
Bbv12I GWGCWC 3 cut(s) 120, 2157, 3114
BbvI GCAGC 4 cut(s) 142, 415, 832, 2084
BccI CCATC 9 cut(s) 799, 1274, 1986, 2276, 2344, 2767, 2783, 3123, 3188
BceAI ACGGC 1 cut(s) 2219
BciT130I CCWGG 4 cut(s) 957, 1515, 2982, 3257
BclI TGATCA 2 cut(s) 694, 2413
BfaI CTAG 7 cut(s) 77, 171, 413, 1367, 2094, 2774, 2840
BfmI CTRYAG 1 cut(s) 1506
BfoI RGCGCY 1 cut(s) 1200
BfrI CTTAAG 1 cut(s) 1759
BfuAI ACCTGC 2 cut(s) 1910, 3055
BglII AGATCT 3 cut(s) 1418, 1763, 1945
BisI GCNGC 5 cut(s) 156, 314, 429, 846, 2098
BlsI GCNGC 5 cut(s) 157, 315, 430, 847, 2099
Bme1390I CCNGG 4 cut(s) 957, 1515, 2982, 3257
Bme18I GGWCC 2 cut(s) 953, 1693
BmeRI GACNNNNNGTC 1 cut(s) 1752
BmeT110I CYCGRG 3 cut(s) 119, 681, 2120
BmgT120I GGNCC 3 cut(s) 265, 953, 1693
BmiI GGNNCC 3 cut(s) 300, 317, 644
BmrFI CCNGG 4 cut(s) 957, 1515, 2982, 3257
BmrI ACTGGG 1 cut(s) 1758
BmuI ACTGGG 1 cut(s) 1758
BoxI GACNNNNGTC 1 cut(s) 440
BpiI GAAGAC 3 cut(s) 277, 1551, 1664
BpmI CTGGAG 1 cut(s) 1583
Bpu10I CCTNAGC 1 cut(s) 1143
BpuEI CTTGAG 4 cut(s) 1750, 1955, 2726, 3365
BsaBI GATNNNNATC 2 cut(s) 903, 1347
BsaI GGTCTC 2 cut(s) 1542, 1898
BsaJI CCNNGG 5 cut(s) 646, 956, 1591, 1987, 2890
BsaXI ACNNNNNCTCC 2 cut(s) 261, 291
Bsc4I CCNNNNNNNGG 6 cut(s) 620, 1394, 1597, 2546, 3121, 3509
Bse1I ACTGG 7 cut(s) 57, 950, 1290, 1753, 3503, 3519, 3532
Bse8I GATNNNNATC 2 cut(s) 903, 1347
BseBI CCWGG 4 cut(s) 957, 1515, 2982, 3257
BseDI CCNNGG 5 cut(s) 646, 956, 1591, 1987, 2890
BseJI GATNNNNATC 2 cut(s) 903, 1347
BseLI CCNNNNNNNGG 6 cut(s) 620, 1394, 1597, 2546, 3121, 3509
BseMII CTCAG 5 cut(s) 429, 1157, 2053, 2198, 2567
BseNI ACTGG 7 cut(s) 57, 950, 1290, 1753, 3503, 3519, 3532
BseRI GAGGAG 2 cut(s) 179, 3491
BseXI GCAGC 4 cut(s) 142, 415, 832, 2084
BseYI CCCAGC 3 cut(s) 262, 1479, 3370
Bsh1236I CGCG 3 cut(s) 43, 45, 2673
BshFI GGCC 4 cut(s) 267, 765, 1707, 2214
BshNI GGYRCC 1 cut(s) 315
BsiHKAI GWGCWC 3 cut(s) 120, 2157, 3114
BsiHKCI CYCGRG 3 cut(s) 119, 681, 2120
BslFI GGGAC 2 cut(s) 1077, 1740
BslI CCNNNNNNNGG 6 cut(s) 620, 1394, 1597, 2546, 3121, 3509
BsmFI GGGAC 2 cut(s) 1077, 1740
BsmI GAATGC 4 cut(s) 582, 2147, 2685, 2927
BsnI GGCC 4 cut(s) 267, 765, 1707, 2214
Bso31I GGTCTC 2 cut(s) 1542, 1898
BsoBI CYCGRG 3 cut(s) 119, 681, 2120
Bsp1286I GDGCHC 3 cut(s) 120, 2157, 3114
Bsp19I CCATGG 1 cut(s) 646
BspACI CCGC 7 cut(s) 45, 313, 580, 655, 862, 1882, 2533
BspANI GGCC 4 cut(s) 267, 765, 1707, 2214
BspCNI CTCAG 5 cut(s) 430, 1156, 2052, 2199, 2568
BspFNI CGCG 3 cut(s) 43, 45, 2673
BspHI TCATGA 5 cut(s) 586, 1492, 1540, 1798, 2995
BspLI GGNNCC 3 cut(s) 300, 317, 644
BspMI ACCTGC 2 cut(s) 1910, 3055
BspPI GGATC 9 cut(s) 293, 306, 637, 650, 714, 912, 1356, 1505, 2476
BspT107I GGYRCC 1 cut(s) 315
BspTI CTTAAG 1 cut(s) 1759
BspTNI GGTCTC 2 cut(s) 1542, 1898
BsrI ACTGG 7 cut(s) 57, 950, 1290, 1753, 3503, 3519, 3532
BssECI CCNNGG 5 cut(s) 646, 956, 1591, 1987, 2890
BssNAI GTATAC 1 cut(s) 537
BssSI CACGAG 1 cut(s) 1163
BssT1I CCWWGG 4 cut(s) 646, 1591, 1987, 2890
Bst1107I GTATAC 1 cut(s) 537
Bst2BI CACGAG 1 cut(s) 1163
Bst2UI CCWGG 4 cut(s) 957, 1515, 2982, 3257
Bst4CI ACNGT 3 cut(s) 1046, 1449, 1500
Bst6I CTCTTC 2 cut(s) 3193, 3546
BstAFI CTTAAG 1 cut(s) 1759
BstAPI GCANNNNNTGC 3 cut(s) 1462, 3421, 3540
BstC8I GCNNGC 8 cut(s) 461, 653, 934, 1324, 1379, 2537, 3228, 3545
BstDSI CCRYGG 1 cut(s) 646
BstEII GGTNACC 1 cut(s) 2638
BstFNI CGCG 3 cut(s) 43, 45, 2673
BstH2I RGCGCY 1 cut(s) 1200
BstHHI GCGC 2 cut(s) 45, 1199
BstMWI GCNNNNNNNGC 7 cut(s) 425, 657, 1462, 2701, 3421, 3531, 3540
BstNI CCWGG 4 cut(s) 957, 1515, 2982, 3257
BstNSI RCATGY 3 cut(s) 467, 655, 1716
BstPAI GACNNNNGTC 1 cut(s) 440
BstPI GGTNACC 1 cut(s) 2638
BstSCI CCNGG 4 cut(s) 955, 1513, 2980, 3255
BstSFI CTRYAG 1 cut(s) 1506
BstUI CGCG 3 cut(s) 43, 45, 2673
BstV1I GCAGC 4 cut(s) 142, 415, 832, 2084
BstV2I GAAGAC 3 cut(s) 277, 1551, 1664
BstX2I RGATCY 7 cut(s) 298, 642, 1418, 1510, 1763, 1945, 2468
BstXI CCANNNNNNTGG 1 cut(s) 3370
BstYI RGATCY 7 cut(s) 298, 642, 1418, 1510, 1763, 1945, 2468
BstZ17I GTATAC 1 cut(s) 537
BsuRI GGCC 4 cut(s) 267, 765, 1707, 2214
BtgI CCRYGG 1 cut(s) 646
BtsI GCAGTG 1 cut(s) 3463
BtsIMutI CAGTG 4 cut(s) 2801, 3463, 3525, 3526
BveI ACCTGC 2 cut(s) 1910, 3055
Cac8I GCNNGC 8 cut(s) 461, 653, 934, 1324, 1379, 2537, 3228, 3545
CaiI CAGNNNCTG 1 cut(s) 845
CciI TCATGA 5 cut(s) 586, 1492, 1540, 1798, 2995
CfoI GCGC 2 cut(s) 45, 1199
Cfr13I GGNCC 3 cut(s) 265, 953, 1693
Csp6I GTAC 3 cut(s) 2796, 3101, 3312
CviQI GTAC 3 cut(s) 2796, 3101, 3312
DraI TTTAAA 2 cut(s) 1783, 2475
DriI GACNNNNNGTC 1 cut(s) 1752
Eam1104I CTCTTC 2 cut(s) 3193, 3546
Eam1105I GACNNNNNGTC 1 cut(s) 1752
EarI CTCTTC 2 cut(s) 3193, 3546
EciI GGCGGA 1 cut(s) 1871
Ecl136II GAGCTC 1 cut(s) 118
Eco130I CCWWGG 4 cut(s) 646, 1591, 1987, 2890
Eco147I AGGCCT 1 cut(s) 1707
Eco24I GRGCYC 1 cut(s) 120
Eco31I GGTCTC 2 cut(s) 1542, 1898
Eco47I GGWCC 2 cut(s) 953, 1693
Eco47III AGCGCT 1 cut(s) 1198
Eco53kI GAGCTC 1 cut(s) 118
Eco57I CTGAAG 3 cut(s) 2664, 2942, 3206
Eco88I CYCGRG 3 cut(s) 119, 681, 2120
Eco91I GGTNACC 1 cut(s) 2638
EcoICRI GAGCTC 1 cut(s) 118
EcoO65I GGTNACC 1 cut(s) 2638
EcoRI GAATTC 4 cut(s) 90, 966, 2615, 3143
EcoRII CCWGG 4 cut(s) 955, 1513, 2980, 3255
EcoT14I CCWWGG 4 cut(s) 646, 1591, 1987, 2890
EcoT22I ATGCAT 3 cut(s) 254, 1458, 2824
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 4 cut(s) 646, 1591, 1987, 2890
FalI AAGNNNNNCTT 2 cut(s) 771, 803
FaqI GGGAC 2 cut(s) 1077, 1740
FauI CCCGC 2 cut(s) 38, 648
FbaI TGATCA 2 cut(s) 694, 2413
FblI GTMKAC 2 cut(s) 536, 2770
Fnu4HI GCNGC 5 cut(s) 156, 314, 429, 846, 2098
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 5 cut(s) 156, 314, 429, 846, 2098
FspBI CTAG 7 cut(s) 77, 171, 413, 1367, 2094, 2774, 2840
GlaI GCGC 2 cut(s) 44, 1198
GluI GCNGC 5 cut(s) 156, 314, 429, 846, 2098
GsaI CCCAGC 3 cut(s) 266, 1483, 3374
GsuI CTGGAG 1 cut(s) 1583
HaeII RGCGCY 1 cut(s) 1200
HaeIII GGCC 4 cut(s) 267, 765, 1707, 2214
HhaI GCGC 2 cut(s) 45, 1199
Hin6I GCGC 2 cut(s) 43, 1197
HinP1I GCGC 2 cut(s) 43, 1197
HincII GTYRAC 3 cut(s) 553, 912, 1611
HindII GTYRAC 3 cut(s) 553, 912, 1611
HindIII AAGCTT 2 cut(s) 2003, 2702
HphI GGTGA 3 cut(s) 88, 2606, 3263
Hpy166II GTNNAC 8 cut(s) 74, 537, 553, 614, 912, 1611, 1693, 2771
Hpy8I GTNNAC 8 cut(s) 74, 537, 553, 614, 912, 1611, 1693, 2771
HpyCH4III ACNGT 3 cut(s) 1046, 1449, 1500
HpyCH4IV ACGT 3 cut(s) 610, 1522, 2564
HpyF10VI GCNNNNNNNGC 7 cut(s) 425, 657, 1462, 2701, 3421, 3531, 3540
HpySE526I ACGT 3 cut(s) 610, 1522, 2564
HspAI GCGC 2 cut(s) 43, 1197
Ksp22I TGATCA 2 cut(s) 694, 2413
LmnI GCTCC 2 cut(s) 842, 2600
Lsp1109I GCAGC 4 cut(s) 142, 415, 832, 2084
MaeI CTAG 7 cut(s) 77, 171, 413, 1367, 2094, 2774, 2840
MaeII ACGT 3 cut(s) 610, 1522, 2564
MaeIII GTNAC 3 cut(s) 1354, 2621, 2638
MfeI CAATTG 4 cut(s) 159, 2196, 2236, 2866
MflI RGATCY 7 cut(s) 298, 642, 1418, 1510, 1763, 1945, 2468
MhlI GDGCHC 3 cut(s) 120, 2157, 3114
MlyI GAGTC 5 cut(s) 176, 1728, 2053, 3073, 3405
MmeI TCCRAC 2 cut(s) 1461, 2110
Mph1103I ATGCAT 3 cut(s) 254, 1458, 2824
MroXI GAANNNNTTC 6 cut(s) 786, 1056, 1488, 1582, 2927, 2964
MslI CAYNNNNRTG 2 cut(s) 101, 569
MspCI CTTAAG 1 cut(s) 1759
MspR9I CCNGG 4 cut(s) 957, 1515, 2982, 3257
MunI CAATTG 4 cut(s) 159, 2196, 2236, 2866
Mva1269I GAATGC 4 cut(s) 582, 2147, 2685, 2927
MvaI CCWGG 4 cut(s) 957, 1515, 2982, 3257
MvnI CGCG 3 cut(s) 43, 45, 2673
MwoI GCNNNNNNNGC 7 cut(s) 425, 657, 1462, 2701, 3421, 3531, 3540
NcoI CCATGG 1 cut(s) 646
NlaIV GGNNCC 3 cut(s) 300, 317, 644
NmeAIII GCCGAG 1 cut(s) 2301
NmuCI GTSAC 1 cut(s) 1354
NsiI ATGCAT 3 cut(s) 254, 1458, 2824
NspI RCATGY 3 cut(s) 467, 655, 1716
PaeI GCATGC 1 cut(s) 655
PaeR7I CTCGAG 2 cut(s) 119, 681
PagI TCATGA 5 cut(s) 586, 1492, 1540, 1798, 2995
PaqCI CACCTGC 1 cut(s) 1910
PceI AGGCCT 1 cut(s) 1707
PciI ACATGT 1 cut(s) 1712
PctI GAATGC 4 cut(s) 582, 2147, 2685, 2927
PdmI GAANNNNTTC 6 cut(s) 786, 1056, 1488, 1582, 2927, 2964
PflMI CCANNNNNTGG 3 cut(s) 620, 1597, 3121
PfoI TCCNGGA 1 cut(s) 2980
PkrI GCNGC 5 cut(s) 157, 315, 430, 847, 2099
PleI GAGTC 5 cut(s) 175, 1728, 2052, 3072, 3404
PpsI GAGTC 5 cut(s) 175, 1728, 2052, 3072, 3404
PscI ACATGT 1 cut(s) 1712
PshAI GACNNNNGTC 1 cut(s) 440
PshBI ATTAAT 1 cut(s) 2084
Psp124BI GAGCTC 1 cut(s) 120
Psp6I CCWGG 4 cut(s) 955, 1513, 2980, 3255
PspEI GGTNACC 1 cut(s) 2638
PspFI CCCAGC 3 cut(s) 262, 1479, 3370
PspGI CCWGG 4 cut(s) 955, 1513, 2980, 3255
PspN4I GGNNCC 3 cut(s) 300, 317, 644
PspPI GGNCC 3 cut(s) 265, 953, 1693
PspXI VCTCGAGB 1 cut(s) 681
PstNI CAGNNNCTG 1 cut(s) 845
PsuI RGATCY 7 cut(s) 298, 642, 1418, 1510, 1763, 1945, 2468
RsaI GTAC 3 cut(s) 2797, 3102, 3313
RsaNI GTAC 3 cut(s) 2796, 3101, 3312
RseI CAYNNNNRTG 2 cut(s) 101, 569
SacI GAGCTC 1 cut(s) 120
SatI GCNGC 5 cut(s) 156, 314, 429, 846, 2098
Sau96I GGNCC 3 cut(s) 265, 953, 1693
SchI GAGTC 5 cut(s) 176, 1728, 2053, 3073, 3405
ScrFI CCNGG 4 cut(s) 957, 1515, 2982, 3257
SduI GDGCHC 3 cut(s) 120, 2157, 3114
SfcI CTRYAG 1 cut(s) 1506
Sfr274I CTCGAG 2 cut(s) 119, 681
SinI GGWCC 2 cut(s) 953, 1693
SlaI CTCGAG 2 cut(s) 119, 681
SmiMI CAYNNNNRTG 2 cut(s) 101, 569
SmlI CTYRAG 7 cut(s) 119, 681, 1729, 1759, 1934, 2705, 3344
SmoI CTYRAG 7 cut(s) 119, 681, 1729, 1759, 1934, 2705, 3344
SphI GCATGC 1 cut(s) 655
SseBI AGGCCT 1 cut(s) 1707
SsiI CCGC 7 cut(s) 45, 313, 580, 655, 862, 1882, 2533
SspI AATATT 3 cut(s) 1308, 2426, 2992
SspMI CTAG 7 cut(s) 77, 171, 413, 1367, 2094, 2774, 2840
SstI GAGCTC 1 cut(s) 120
StuI AGGCCT 1 cut(s) 1707
StyD4I CCNGG 4 cut(s) 955, 1513, 2980, 3255
StyI CCWWGG 4 cut(s) 646, 1591, 1987, 2890
TaaI ACNGT 3 cut(s) 1046, 1449, 1500
TaiI ACGT 3 cut(s) 613, 1525, 2567
TaqI TCGA 6 cut(s) 120, 682, 1451, 1857, 2689, 3192
TaqII GACCGA 2 cut(s) 126, 732
TatI WGTACW 2 cut(s) 2795, 3100
TauI GCSGC 1 cut(s) 316
TscAI CASTG 4 cut(s) 2808, 3463, 3526, 3532
TseFI GTSAC 1 cut(s) 1354
TseI GCWGC 4 cut(s) 155, 428, 845, 2097
Tsp45I GTSAC 1 cut(s) 1354
TspGWI ACGGA 4 cut(s) 211, 520, 1143, 3389
TspRI CASTG 4 cut(s) 2808, 3463, 3526, 3532
Van91I CCANNNNNTGG 3 cut(s) 620, 1597, 3121
Vha464I CTTAAG 1 cut(s) 1759
VpaK11BI GGWCC 2 cut(s) 953, 1693
VspI ATTAAT 1 cut(s) 2084
XbaI TCTAGA 1 cut(s) 170
XceI RCATGY 3 cut(s) 467, 655, 1716
XcmI CCANNNNNNNNNTGG 2 cut(s) 802, 1985
XhoI CTCGAG 2 cut(s) 119, 681
XmiI GTMKAC 2 cut(s) 536, 2770
XmnI GAANNNNTTC 6 cut(s) 786, 1056, 1488, 1582, 2927, 2964
XspI CTAG 7 cut(s) 77, 171, 413, 1367, 2094, 2774, 2840
Zsp2I ATGCAT 3 cut(s) 254, 1458, 2824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.