Rw1G004660

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Reverse (-)
8950324 .. 8954539
4216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004660.1

Sequence Viewer

Length: 3687 bp
ATGGCGTCAACCACTTCTCGTCCTCCATGGAAATACGATGTGTTTCTAAGTTTCAGGGGGGAAGATACCCGCAATAATTTTACCGATCATCTGTATTCTACACTAAATCAGAGAGGAATTCTCACCTTCCGGGATGACCCAGAGCTTGAAAAAGGAAAATCCATTGGGCCTGAACTTTTTGCTGCAATTGAGCAGTCCAGATGTGCGATTGTCATTCTCTCACCCAGATATGCTGAGTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAAGGGGGGGCCAACAAGTCCTCCCCGTCTTCTACGGGGTGGATCCTTCTGAAGTGCGGCACCAAAGGGGAAGTTTTGAGCTCAAAAGGGAAACCCAAGTAGAGGTGAAAGTAAAGGAACATGAAGAAGTTTATACGAGGAACGGGGACAGACTAAATGCCTGGAGAGCTGCTTTGACACAAGTCGCCAATCTTTCTGGCTGGGATGCCGAGAATTATCGGTATGGAAAACAAAAAATGATTGAGGATATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATATATTAAGCAATTTCATATATCCGCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGGATAGGTAAGACCACACTTGCTCGAGCTATCCATGATGAAATTTGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAACCAATGGCCTTGTTTCTCTACAAGAAAAACTTCTTTCCAAAATCCTGATGGCAAAAGTTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATACAAAGGCGGTTGTGTAGAATCAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACACAATTAGAGAAATTGGCTGGCAGTCGCAACTGGTTTGGTCCAGGGAGTAGAATCATCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGACGTTGATGCTACATACAAGGCTAACAGGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAGGGCTTTTAAGAAATGTCCCCCACCAGAAGATTATTTGGATCTGTGCTACCATATTCTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCGCTGATAAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGACAAACGCATTATTGGGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACAGAGAAATATTCCTGCATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAGACCCTGTCATTGGTTTAGATGTTCTTGCTGATAGATCTCTCATAACCATCTCCAACAACGAACTGTTGATGCATGATTTACTACAAGCAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTGAATAGAAATACGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTACATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTGCTCCATATTCGTAATGTGGACCTACCCAAAGGCCTCACCTGTCTTTCTAATTCCTTGAGACTTCTTGAATGGACTGGGTGTCCCTTAAGATCTCTCCCACAAAACTTTGAAGCCGATGAACTTGTTGAACTTAACTTGTGCCACAGCAACATTGAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCGTTTGGTAAGAATCCATCAATCCCTTGCATTTCTCAAAAAGCTTATTGTGCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTGCCAAGTAGAATTGAAATGGAGTCTCTTGAATCATTAATTCTTTCTAACTGTTCAAAAGTTAAGAAGATTCCTGAGTTTGTTGGAAATATGGAATGTTTGTTGGTGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCTGATTCAATTGAACGGCTGAGTGGTCTTGTGTCATTGAATCTACGCAACTGCAGAAATCTTGTTTGTCTTCCTAGCACCATCAATAAATTGAAGTCTATTGAAGATCTTAATCTTTCTGGATGCTTGAACCTCGGCAAACCTCAGGTAAATGTGGGGGTGATGGGCTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGCCATCTACCCATGATCGCAAGAAAGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGTCGTCTACCCATGATCGCAAGAAAAAAGGGAGAGGTCCAATCTTTCAAGGATGCAAAGTAGTTTGGCGATCTTTCAAAAAGTTCTTGCCTTCTAGATTGGTGCAAAAAGTGAATACAGAGCCAAAGAGCTTCCACTTGCCTATATCACAAAACGTGAATGCAGAGCCAAAGAGTTTCCACTTGCCTATATCACAAAACGTGAATGCAGAGCCAACGATTTTCCGCTTGCCTATTTCTGGTCTCTGTAATTTAACGTATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTCGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGTGTTCCTTCAGGCATTCGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTGAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCTTATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGACGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCCAATAACAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAATGGTTCAGCCATTGGAGTGCTGGGCGCTCTGTAAGTGTATCTCTGCCTGCACATTGGAATAACAGTCGGGTTTTGGGATTTGCTCTGTGTGCTGTTTTTGTACTCCATGAGCAACAGCGGAATGAAAAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCGATGGAAGAGAATTGGAAGTATATGGCAGACATCCTGCATTTCGCTTTAGTGAAGAATTTTGCAAGGTTAAGTCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTCGGTACAGAGTGGTGGCATAACAGTTGCAGTAAGTTTGAGTTCTTATTTGAAACCAGAGGGCCAGGTCTGCAGGTGAAGGGATGTGGAGTCCATCTGATATATGAGCAAGATGTGCCAGAGTTGAACCAAACAACCACTCAATCAAGGCGTGGGGAAACCAGTAGCAGTGGTAGCAGAACTTGCACGCTTGAAGAATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1228

Amino Acids

139.42

Weight (kDa)

6.98

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 192 7e-51 TIR domain
TIR_2 PF13676 14 - 111 1.9e-15 TIR domain
NB-ARC PF00931 218 - 372 3.9e-19 NB-ARC domain
AAA_22 PF13401 220 - 318 1e-05 AAA domain
NACHT PF05729 222 - 319 5.1e-07 NACHT domain
WHD_ROQ1 PF23282 439 - 510 2.2e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 608 - 626 5.2e-07 Leucine Rich Repeat
LRR_13 PF23286 699 - 783 1.2e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 710 - 778 1.7e-09 Leucine-rich repeat region
C-JID PF20160 1052 - 1200 2.8e-18 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1937, 3547
Acc36I ACCTGC 3 cut(s) 758, 1937, 3547
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 1 cut(s) 1624
AccI GTMKAC 3 cut(s) 563, 2492, 2947
AciI CCGC 8 cut(s) 70, 340, 607, 641, 682, 889, 2710, 3295
AclWI GGATC 7 cut(s) 320, 333, 664, 677, 939, 1149, 1383
AcsI RAATTY 8 cut(s) 117, 726, 1681, 1879, 2792, 3004, 3320, 3425
AcuI CTGAAG 4 cut(s) 354, 2841, 3119, 3383
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 2974, 3279, 3490
AfeI AGCGCT 1 cut(s) 1225
AfiI CCNNNNNNNGG 6 cut(s) 385, 647, 1421, 1624, 2723, 3132
AflII CTTAAG 1 cut(s) 1786
AhdI GACNNNNNGTC 1 cut(s) 1779
AjnI CCWGG 5 cut(s) 443, 982, 1540, 3157, 3547
AjuI GAANNNNNNNTTGG 2 cut(s) 2879, 2911
Alw21I GWGCWC 1 cut(s) 366
AlwI GGATC 7 cut(s) 320, 333, 664, 677, 939, 1149, 1383
AlwNI CAGNNNCTG 1 cut(s) 872
Ama87I CYCGRG 2 cut(s) 708, 2066
Aor51HI AGCGCT 1 cut(s) 1225
AoxI GGCC 5 cut(s) 167, 292, 790, 1732, 3545
ApeKI GCWGC 4 cut(s) 182, 452, 643, 872
ApoI RAATTY 8 cut(s) 117, 726, 1681, 1879, 2792, 3004, 3320, 3425
AseI ATTAAT 1 cut(s) 2111
Asp700I GAANNNNTTC 7 cut(s) 601, 813, 1083, 1515, 1609, 3104, 3141
AspLEI GCGC 2 cut(s) 1226, 3204
AspS9I GGNCC 6 cut(s) 167, 292, 980, 1720, 2522, 3545
AsuC2I CCSGG 1 cut(s) 131
AsuHPI GGTGA 9 cut(s) 115, 213, 400, 1729, 2386, 2783, 2827, 3440, 3571
AvaI CYCGRG 2 cut(s) 708, 2066
AvaII GGWCC 3 cut(s) 980, 1720, 2522
AxyI CCTNAGG 1 cut(s) 2358
BamHI GGATCC 2 cut(s) 325, 669
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 366
BauI CACGAG 2 cut(s) 1190, 3471
BbsI GAAGAC 4 cut(s) 304, 1578, 2276, 3107
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 4 cut(s) 169, 439, 655, 859
BceAI ACGGC 1 cut(s) 2246
BcgI CGANNNNNNTGC 2 cut(s) 2047, 2081
BciT130I CCWGG 5 cut(s) 445, 984, 1542, 3159, 3549
BcnI CCSGG 1 cut(s) 131
BfaI CTAG 6 cut(s) 1160, 1394, 1409, 2289, 2580, 2951
BfmI CTRYAG 2 cut(s) 2266, 3554
BfoI RGCGCY 2 cut(s) 1227, 3205
BfrI CTTAAG 1 cut(s) 1786
BfuAI ACCTGC 3 cut(s) 758, 1937, 3547
BglII AGATCT 4 cut(s) 1445, 1790, 1972, 2320
BisI GCNGC 5 cut(s) 183, 341, 453, 644, 873
BlsI GCNGC 5 cut(s) 184, 342, 454, 645, 874
Bme1390I CCNGG 6 cut(s) 131, 445, 984, 1542, 3159, 3549
Bme18I GGWCC 3 cut(s) 980, 1720, 2522
BmeRI GACNNNNNGTC 1 cut(s) 1779
BmeT110I CYCGRG 2 cut(s) 708, 2066
BmgT120I GGNCC 6 cut(s) 167, 292, 980, 1720, 2522, 3545
BmiI GGNNCC 4 cut(s) 293, 327, 344, 671
BmrFI CCNGG 6 cut(s) 131, 445, 984, 1542, 3159, 3549
BmrI ACTGGG 1 cut(s) 1785
BmsI GCATC 7 cut(s) 478, 1033, 1470, 2327, 2528, 2986, 3008
BmuI ACTGGG 1 cut(s) 1785
BoxI GACNNNNGTC 1 cut(s) 464
BpiI GAAGAC 4 cut(s) 304, 1578, 2276, 3107
BplI GAGNNNNNCTC 2 cut(s) 105, 137
BpmI CTGGAG 1 cut(s) 466
Bpu10I CCTNAGC 1 cut(s) 1170
BpuEI CTTGAG 3 cut(s) 1777, 1982, 2903
BpuMI CCSGG 1 cut(s) 131
BsaBI GATNNNNATC 3 cut(s) 930, 1374, 2325
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 3 cut(s) 1569, 1925, 2732
BsaJI CCNNGG 5 cut(s) 26, 673, 983, 1618, 2347
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 6 cut(s) 385, 647, 1421, 1624, 2723, 3132
Bse1I ACTGG 4 cut(s) 977, 1317, 1780, 3645
Bse21I CCTNAGG 1 cut(s) 2358
Bse3DI GCAATG 1 cut(s) 1506
Bse8I GATNNNNATC 3 cut(s) 930, 1374, 2325
BseBI CCWGG 5 cut(s) 445, 984, 1542, 3159, 3549
BseDI CCNNGG 5 cut(s) 26, 673, 983, 1618, 2347
BseJI GATNNNNATC 3 cut(s) 930, 1374, 2325
BseLI CCNNNNNNNGG 6 cut(s) 385, 647, 1421, 1624, 2723, 3132
BseMI GCAATG 1 cut(s) 1506
BseMII CTCAG 6 cut(s) 225, 1184, 2139, 2225, 2372, 2744
BseNI ACTGG 4 cut(s) 977, 1317, 1780, 3645
BseXI GCAGC 4 cut(s) 169, 439, 655, 859
BseYI CCCAGC 3 cut(s) 483, 1506, 3197
BsgI GTGCAG 1 cut(s) 3210
BshFI GGCC 5 cut(s) 169, 294, 792, 1734, 3547
BshNI GGYRCC 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 2 cut(s) 708, 2066
BsiSI CCGG 1 cut(s) 130
BslFI GGGAC 3 cut(s) 443, 1104, 1767
BslI CCNNNNNNNGG 6 cut(s) 385, 647, 1421, 1624, 2723, 3132
BsmFI GGGAC 3 cut(s) 443, 1104, 1767
BsmI GAATGC 3 cut(s) 2650, 2695, 2862
BsnI GGCC 5 cut(s) 169, 294, 792, 1734, 3547
Bso31I GGTCTC 3 cut(s) 1569, 1925, 2732
BsoBI CYCGRG 2 cut(s) 708, 2066
Bsp1286I GDGCHC 1 cut(s) 366
Bsp19I CCATGG 2 cut(s) 26, 673
BspACI CCGC 8 cut(s) 70, 340, 607, 641, 682, 889, 2710, 3295
BspANI GGCC 5 cut(s) 169, 294, 792, 1734, 3547
BspCNI CTCAG 6 cut(s) 226, 1183, 2140, 2226, 2371, 2745
BspHI TCATGA 1 cut(s) 1567
BspLI GGNNCC 4 cut(s) 293, 327, 344, 671
BspMAI CTGCAG 2 cut(s) 2270, 3558
BspMI ACCTGC 3 cut(s) 758, 1937, 3547
BspPI GGATC 7 cut(s) 320, 333, 664, 677, 939, 1149, 1383
BspT107I GGYRCC 1 cut(s) 342
BspTI CTTAAG 1 cut(s) 1786
BspTNI GGTCTC 3 cut(s) 1569, 1925, 2732
BsrDI GCAATG 1 cut(s) 1506
BsrI ACTGG 4 cut(s) 977, 1317, 1780, 3645
BssECI CCNNGG 5 cut(s) 26, 673, 983, 1618, 2347
BssNAI GTATAC 1 cut(s) 564
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 2 cut(s) 1190, 3471
BssT1I CCWWGG 3 cut(s) 26, 673, 1618
Bst1107I GTATAC 1 cut(s) 564
Bst2BI CACGAG 2 cut(s) 1190, 3471
Bst2UI CCWGG 5 cut(s) 445, 984, 1542, 3159, 3549
Bst4CI ACNGT 6 cut(s) 1073, 1476, 1527, 2126, 3242, 3509
Bst6I CTCTTC 2 cut(s) 2913, 3370
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 1786
BstAPI GCANNNNNTGC 2 cut(s) 3598, 3666
BstC8I GCNNGC 7 cut(s) 680, 961, 1351, 1406, 2714, 3225, 3671
BstDSI CCRYGG 2 cut(s) 26, 673
BstEII GGTNACC 1 cut(s) 2815
BstH2I RGCGCY 2 cut(s) 1227, 3205
BstHHI GCGC 2 cut(s) 1226, 3204
BstMWI GCNNNNNNNGC 8 cut(s) 449, 2038, 2878, 3266, 3553, 3598, 3657, 3666
BstNI CCWGG 5 cut(s) 445, 984, 1542, 3159, 3549
BstNSI RCATGY 1 cut(s) 682
BstPAI GACNNNNGTC 1 cut(s) 464
BstPI GGTNACC 1 cut(s) 2815
BstSCI CCNGG 6 cut(s) 129, 443, 982, 1540, 3157, 3547
BstSFI CTRYAG 2 cut(s) 2266, 3554
BstV1I GCAGC 4 cut(s) 169, 439, 655, 859
BstV2I GAAGAC 4 cut(s) 304, 1578, 2276, 3107
BstX2I RGATCY 7 cut(s) 325, 669, 1141, 1445, 1790, 1972, 2320
BstYI RGATCY 7 cut(s) 325, 669, 1141, 1445, 1790, 1972, 2320
BstZ17I GTATAC 1 cut(s) 564
Bsu36I CCTNAGG 1 cut(s) 2358
BsuRI GGCC 5 cut(s) 169, 294, 792, 1734, 3547
BtgI CCRYGG 2 cut(s) 26, 673
BtsI GCAGTG 1 cut(s) 3658
BtsIMutI CAGTG 2 cut(s) 2978, 3658
BveI ACCTGC 3 cut(s) 758, 1937, 3547
Cac8I GCNNGC 7 cut(s) 680, 961, 1351, 1406, 2714, 3225, 3671
CaiI CAGNNNCTG 1 cut(s) 872
CciI TCATGA 1 cut(s) 1567
CfoI GCGC 2 cut(s) 1226, 3204
Cfr13I GGNCC 6 cut(s) 167, 292, 980, 1720, 2522, 3545
CseI GACGC 1 cut(s) 3081
Csp6I GTAC 3 cut(s) 2973, 3278, 3489
CviQI GTAC 3 cut(s) 2973, 3278, 3489
DriI GACNNNNNGTC 1 cut(s) 1779
Eam1104I CTCTTC 2 cut(s) 2913, 3370
Eam1105I GACNNNNNGTC 1 cut(s) 1779
EarI CTCTTC 2 cut(s) 2913, 3370
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 3 cut(s) 26, 673, 1618
Eco147I AGGCCT 1 cut(s) 1734
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 3 cut(s) 1569, 1925, 2732
Eco47I GGWCC 3 cut(s) 980, 1720, 2522
Eco47III AGCGCT 1 cut(s) 1225
Eco53kI GAGCTC 1 cut(s) 364
Eco57I CTGAAG 4 cut(s) 354, 2841, 3119, 3383
Eco81I CCTNAGG 1 cut(s) 2358
Eco88I CYCGRG 2 cut(s) 708, 2066
Eco91I GGTNACC 1 cut(s) 2815
EcoICRI GAGCTC 1 cut(s) 364
EcoO65I GGTNACC 1 cut(s) 2815
EcoRI GAATTC 3 cut(s) 117, 2792, 3320
EcoRII CCWGG 5 cut(s) 443, 982, 1540, 3157, 3547
EcoT14I CCWWGG 3 cut(s) 26, 673, 1618
EcoT22I ATGCAT 3 cut(s) 281, 1485, 3001
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 3 cut(s) 26, 673, 1618
FalI AAGNNNNNCTT 2 cut(s) 798, 830
FaqI GGGAC 3 cut(s) 443, 1104, 1767
FauI CCCGC 3 cut(s) 77, 614, 675
FblI GTMKAC 3 cut(s) 563, 2492, 2947
Fnu4HI GCNGC 5 cut(s) 183, 341, 453, 644, 873
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 5 cut(s) 183, 341, 453, 644, 873
FspBI CTAG 6 cut(s) 1160, 1394, 1409, 2289, 2580, 2951
GlaI GCGC 2 cut(s) 1225, 3203
GluI GCNGC 5 cut(s) 183, 341, 453, 644, 873
GsaI CCCAGC 3 cut(s) 487, 1510, 3201
GsuI CTGGAG 1 cut(s) 466
HaeII RGCGCY 2 cut(s) 1227, 3205
HaeIII GGCC 5 cut(s) 169, 294, 792, 1734, 3547
HapII CCGG 1 cut(s) 130
HgaI GACGC 1 cut(s) 3081
HhaI GCGC 2 cut(s) 1226, 3204
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 2 cut(s) 1224, 3202
HinP1I GCGC 2 cut(s) 1224, 3202
HincII GTYRAC 4 cut(s) 9, 580, 939, 1638
HindII GTYRAC 4 cut(s) 9, 580, 939, 1638
HindIII AAGCTT 3 cut(s) 2030, 2879, 3305
HpaII CCGG 1 cut(s) 130
HphI GGTGA 9 cut(s) 115, 213, 400, 1729, 2386, 2783, 2827, 3440, 3571
Hpy166II GTNNAC 9 cut(s) 9, 564, 580, 939, 1523, 1638, 1720, 2493, 2948
Hpy188I TCNGA 8 cut(s) 111, 334, 1296, 1956, 2748, 2929, 3445, 3582
Hpy8I GTNNAC 9 cut(s) 9, 564, 580, 939, 1523, 1638, 1720, 2493, 2948
HpyCH4III ACNGT 6 cut(s) 1073, 1476, 1527, 2126, 3242, 3509
HpyCH4IV ACGT 5 cut(s) 1038, 1549, 2640, 2685, 2741
HpyF10VI GCNNNNNNNGC 8 cut(s) 449, 2038, 2878, 3266, 3553, 3598, 3657, 3666
HpySE526I ACGT 5 cut(s) 1038, 1549, 2640, 2685, 2741
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 2 cut(s) 1224, 3202
LmnI GCTCC 3 cut(s) 869, 1707, 2777
Lsp1109I GCAGC 4 cut(s) 169, 439, 655, 859
LweI GCATC 7 cut(s) 478, 1033, 1470, 2327, 2528, 2986, 3008
MaeI CTAG 6 cut(s) 1160, 1394, 1409, 2289, 2580, 2951
MaeII ACGT 5 cut(s) 1038, 1549, 2640, 2685, 2741
MaeIII GTNAC 3 cut(s) 1381, 2798, 2815
MfeI CAATTG 3 cut(s) 186, 2223, 3043
MflI RGATCY 7 cut(s) 325, 669, 1141, 1445, 1790, 1972, 2320
MhlI GDGCHC 1 cut(s) 366
MlyI GAGTC 4 cut(s) 245, 2080, 2105, 3582
MmeI TCCRAC 2 cut(s) 1488, 2137
Mph1103I ATGCAT 3 cut(s) 281, 1485, 3001
MroXI GAANNNNTTC 7 cut(s) 601, 813, 1083, 1515, 1609, 3104, 3141
MslI CAYNNNNRTG 3 cut(s) 596, 1628, 3192
MspA1I CMGCKG 1 cut(s) 3295
MspCI CTTAAG 1 cut(s) 1786
MspI CCGG 1 cut(s) 130
MspR9I CCNGG 6 cut(s) 131, 445, 984, 1542, 3159, 3549
MunI CAATTG 3 cut(s) 186, 2223, 3043
Mva1269I GAATGC 3 cut(s) 2650, 2695, 2862
MvaI CCWGG 5 cut(s) 445, 984, 1542, 3159, 3549
MwoI GCNNNNNNNGC 8 cut(s) 449, 2038, 2878, 3266, 3553, 3598, 3657, 3666
NciI CCSGG 1 cut(s) 131
NcoI CCATGG 2 cut(s) 26, 673
NlaIV GGNNCC 4 cut(s) 293, 327, 344, 671
NmeAIII GCCGAG 2 cut(s) 517, 2328
NmuCI GTSAC 2 cut(s) 1381, 2815
NsiI ATGCAT 3 cut(s) 281, 1485, 3001
NspI RCATGY 1 cut(s) 682
PaeI GCATGC 1 cut(s) 682
PaeR7I CTCGAG 2 cut(s) 708, 2066
PagI TCATGA 1 cut(s) 1567
PaqCI CACCTGC 2 cut(s) 1937, 3547
PceI AGGCCT 1 cut(s) 1734
PctI GAATGC 3 cut(s) 2650, 2695, 2862
PdmI GAANNNNTTC 7 cut(s) 601, 813, 1083, 1515, 1609, 3104, 3141
PflFI GACNNNGTC 1 cut(s) 1415
PflMI CCANNNNNTGG 1 cut(s) 1624
PfoI TCCNGGA 2 cut(s) 129, 3157
PkrI GCNGC 5 cut(s) 184, 342, 454, 645, 874
PleI GAGTC 4 cut(s) 244, 2079, 2104, 3581
PpsI GAGTC 4 cut(s) 244, 2079, 2104, 3581
PshAI GACNNNNGTC 1 cut(s) 464
PshBI ATTAAT 1 cut(s) 2111
Psp124BI GAGCTC 1 cut(s) 366
Psp6I CCWGG 5 cut(s) 443, 982, 1540, 3157, 3547
PspEI GGTNACC 1 cut(s) 2815
PspFI CCCAGC 3 cut(s) 483, 1506, 3197
PspGI CCWGG 5 cut(s) 443, 982, 1540, 3157, 3547
PspN4I GGNNCC 4 cut(s) 293, 327, 344, 671
PspPI GGNCC 6 cut(s) 167, 292, 980, 1720, 2522, 3545
PspXI VCTCGAGB 1 cut(s) 708
PstI CTGCAG 2 cut(s) 2270, 3558
PstNI CAGNNNCTG 1 cut(s) 872
PsuI RGATCY 7 cut(s) 325, 669, 1141, 1445, 1790, 1972, 2320
PsyI GACNNNGTC 1 cut(s) 1415
RsaI GTAC 3 cut(s) 2974, 3279, 3490
RsaNI GTAC 3 cut(s) 2973, 3278, 3489
RseI CAYNNNNRTG 3 cut(s) 596, 1628, 3192
SacI GAGCTC 1 cut(s) 366
SatI GCNGC 5 cut(s) 183, 341, 453, 644, 873
Sau96I GGNCC 6 cut(s) 167, 292, 980, 1720, 2522, 3545
SchI GAGTC 4 cut(s) 245, 2080, 2105, 3582
ScrFI CCNGG 6 cut(s) 131, 445, 984, 1542, 3159, 3549
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 7 cut(s) 478, 1033, 1470, 2327, 2528, 2986, 3008
SfcI CTRYAG 2 cut(s) 2266, 3554
Sfr274I CTCGAG 2 cut(s) 708, 2066
SinI GGWCC 3 cut(s) 980, 1720, 2522
SlaI CTCGAG 2 cut(s) 708, 2066
SmiMI CAYNNNNRTG 3 cut(s) 596, 1628, 3192
SmlI CTYRAG 6 cut(s) 708, 1756, 1786, 1961, 2066, 2882
SmoI CTYRAG 6 cut(s) 708, 1756, 1786, 1961, 2066, 2882
SphI GCATGC 1 cut(s) 682
SseBI AGGCCT 1 cut(s) 1734
SsiI CCGC 8 cut(s) 70, 340, 607, 641, 682, 889, 2710, 3295
SspI AATATT 2 cut(s) 1335, 3169
SspMI CTAG 6 cut(s) 1160, 1394, 1409, 2289, 2580, 2951
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 1734
StyD4I CCNGG 6 cut(s) 129, 443, 982, 1540, 3157, 3547
StyI CCWWGG 3 cut(s) 26, 673, 1618
TaaI ACNGT 6 cut(s) 1073, 1476, 1527, 2126, 3242, 3509
TaiI ACGT 5 cut(s) 1041, 1552, 2643, 2688, 2744
TaqI TCGA 4 cut(s) 709, 2067, 2866, 3369
TaqII GACCGA 1 cut(s) 759
TatI WGTACW 2 cut(s) 2972, 3277
TauI GCSGC 1 cut(s) 343
TscAI CASTG 2 cut(s) 2985, 3658
TseFI GTSAC 2 cut(s) 1381, 2815
TseI GCWGC 4 cut(s) 182, 452, 643, 872
Tsp45I GTSAC 2 cut(s) 1381, 2815
TspGWI ACGGA 2 cut(s) 547, 1170
TspRI CASTG 2 cut(s) 2985, 3658
Tth111I GACNNNGTC 1 cut(s) 1415
Van91I CCANNNNNTGG 1 cut(s) 1624
Vha464I CTTAAG 1 cut(s) 1786
VpaK11BI GGWCC 3 cut(s) 980, 1720, 2522
VspI ATTAAT 1 cut(s) 2111
XapI RAATTY 8 cut(s) 117, 726, 1681, 1879, 2792, 3004, 3320, 3425
XbaI TCTAGA 1 cut(s) 2579
XceI RCATGY 1 cut(s) 682
XcmI CCANNNNNNNNNTGG 2 cut(s) 829, 3194
XhoI CTCGAG 2 cut(s) 708, 2066
XmiI GTMKAC 3 cut(s) 563, 2492, 2947
XmnI GAANNNNTTC 7 cut(s) 601, 813, 1083, 1515, 1609, 3104, 3141
XspI CTAG 6 cut(s) 1160, 1394, 1409, 2289, 2580, 2951
Zsp2I ATGCAT 3 cut(s) 281, 1485, 3001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.