RLG00000030322

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000105
Physical Location & Seq
Reverse (-)
59602568 .. 59608666
6099 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030322

Sequence Viewer

Length: 3126 bp
ATGCTAATATTGTTTTTGCCGCTTATGTTACATTGCTTAGTTTCTAACTCACAATTTCATCTGAATTTTCTCAGAAATAATACCGACCTAATTGACGATCTTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCGTCGATGATTTATTAAGCAATTACATATATCCGCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGGATAGGTAAGACAACACTTGCTCGAGCTATCCATGATGAAATATGTCAGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAACCAATGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCAAAATCCTAATGGAAAAAGTTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGAGACAATTAGAGAAATTAGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAAAATCATCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGATGTTGATGCCACATACAAGGTTAACGGGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATATCCCCCACCAGAAGATTATTTGTACCTGTGCAACCTTGTTTTAGGGTATGCTCAGGGGCTTCCATTGGCTCTCGTGGTTTTAGGTTCCTTTCTATTTGGTAGAAGCACTGATAAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAAATAAACACATCATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGATGAAAAAGACCAAGAAATATTCCTACATATTGCTTGCTTTTATAAGGGGAAGCATAAGGATCGTGTGACACAAATACTAGACTATTGCCAGCTAGACCCTGTCATTGGTTTAGATGTTCTTGCTGATAGATCTCTCATAACCATCTCCAATAACGAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTGTCATGAAGACATCAACAATGTGCTGAAGAGAAATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAATTACAAGTGGCTGATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTCGTAATGTGGACCTTCCCAAAGGCCTCACTTGTCTTTCTAATTCCTTAAGACTCCTCGAATGGACTGGGTATCCCTTAAGATCTCTCCCACAAAAATTTGAAGCAGATGAACTTGTTGAACTTAACTTGTGCCACAGCAACATTAAACAGCTTTGGAAGGGAACAAAGAATTTTGACAATTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGCGTTCAGAATCTTGAGGCTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCGAGAGTCTTCCTAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTCAGTGCTTTCTCTTGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACAGCTAAGCGGTCTTGTGTCATTGAATCTGAGCAACTGCAGAAATCTTGTCTATCTTCCGAGCACCATCAATAAATTGAAGTCTATTAAAAATCTTAACCTTTCTGGATGCTTGAAACTTGGCAAACATCAGGTAAATGTGGGGGAGATGGACTGTTTTGAGGAAATGTCAACTACGCACGTTCTTATAAAATATATGACACGTTCAATCTTTCATGGATGTGAAGTAGTTTGGGGATCTGTAAAGAAGTTCTTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGAGCCAATGAGTTTCCACTTGCCTATTTCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGCATTCCTTCAGGCATTGGATTGCTTTCCAAGCTTGAGAACTTTAACTTGGAGAATTGCAGGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTGCAATCTAAATGGCAATCACGAATGCAACAACATAGCATTTGAAATGCTAAAGACATGCATGTATCAGGGAATCTCTAATAAGAGGGAAACTTTTCAAATTGTAATTCCTGGAAATTATATTCCTGAGTGGTTCAGCCACTGGAGTTTTGGGCGTTCATTAAGTGTACCTCTACCTGCAGATTGGAATAACAGTCGATTTTTGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATGAGCACCATCGGGTGGATGAGCTTTATATAAATGAATTCACGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGACGCTCGATGGAAGAGAATTGGAAGTGTACGGCAGACAGCCTGCATTTCGCTTCAGTGAAGAATTTTGCCAGGTTGAGTCGGATCATCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCGTAACAGTTGCAGTCAGCTTGAGTTCTTTTTTGAAACCAGAGGACCGGGTCTGAGGGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAACATGTGCAAGAGTTGAACCAAACAACCACTCAACCAAGCAGTGAGATGTCTCCCTATGAGGATATATTGATTGGTTTTGACATTCCAATTGCAGGGGAAACCAGTGGCACTGGTAGCAGAACTTGCACGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1042

Amino Acids

118.4

Weight (kDa)

5.92

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 74 - 228 4.3e-20 NB-ARC domain
AAA_22 PF13401 76 - 173 7.7e-06 AAA domain
NACHT PF05729 78 - 221 7.8e-08 NACHT domain
WHD_ROQ1 PF23282 295 - 366 3.7e-23 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 464 - 482 2.7e-06 Leucine Rich Repeat
LRR_14 PF23598 495 - 587 2.6e-06 Leucine-rich repeat region
LRR_13 PF23286 555 - 639 1.6e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 574 - 660 4e-08 Leucine-rich repeat region
C-JID PF20160 848 - 996 3.1e-17 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 927, 1985
Acc36I ACCTGC 2 cut(s) 326, 2620
AccB7I CCANNNNNTGG 2 cut(s) 1192, 2686
AccI GTMKAC 2 cut(s) 131, 2335
AccII CGCG 1 cut(s) 177
AciI CCGC 6 cut(s) 20, 175, 209, 250, 457, 1806
AclI AACGTT 1 cut(s) 1738
AclWI GGATC 6 cut(s) 232, 245, 507, 951, 2041, 2841
AcsI RAATTY 9 cut(s) 64, 1249, 1373, 1447, 1558, 2180, 2392, 2708, 2813
AcuI CTGAAG 3 cut(s) 1178, 2229, 2788
AfaI GTAC 6 cut(s) 710, 2362, 2604, 2667, 2780, 2878
AfiI CCNNNNNNNGG 6 cut(s) 215, 989, 1192, 2111, 2686, 3074
AflII CTTAAG 2 cut(s) 1324, 1354
AflIII ACRYGT 2 cut(s) 1997, 2983
AjnI CCWGG 4 cut(s) 550, 1108, 2545, 2820
AjuI GAANNNNNNNTTGG 2 cut(s) 2267, 2299
Alw21I GWGCWC 2 cut(s) 1862, 2679
Alw26I GTCTC 8 cut(s) 502, 1101, 1260, 1493, 1637, 1757, 2293, 3036
AlwI GGATC 6 cut(s) 232, 245, 507, 951, 2041, 2841
AlwNI CAGNNNCTG 2 cut(s) 440, 2577
Ama87I CYCGRG 3 cut(s) 276, 1634, 1715
AoxI GGCC 2 cut(s) 358, 1300
ApeKI GCWGC 3 cut(s) 211, 440, 2436
ApoI RAATTY 9 cut(s) 64, 1249, 1373, 1447, 1558, 2180, 2392, 2708, 2813
AseI ATTAAT 1 cut(s) 1679
Asp700I GAANNNNTTC 6 cut(s) 169, 381, 651, 1083, 1177, 2529
AspS9I GGNCC 3 cut(s) 548, 1288, 2933
AsuC2I CCSGG 1 cut(s) 2937
AsuHPI GGTGA 3 cut(s) 2171, 2215, 2959
AvaI CYCGRG 3 cut(s) 276, 1634, 1715
AvaII GGWCC 3 cut(s) 548, 1288, 2933
BamHI GGATCC 1 cut(s) 237
BauI CACGAG 2 cut(s) 758, 2859
BbsI GAAGAC 3 cut(s) 1146, 1259, 1634
Bbv12I GWGCWC 2 cut(s) 1862, 2679
BbvI GCAGC 3 cut(s) 223, 427, 2423
BccI CCATC 9 cut(s) 869, 1034, 1581, 1871, 1939, 2332, 2348, 2688, 2753
BceAI ACGGC 1 cut(s) 2797
BcgI CGANNNNNNTGC 4 cut(s) 926, 960, 1615, 1649
BciT130I CCWGG 4 cut(s) 552, 1110, 2547, 2822
BciVI GTATCC 1 cut(s) 1359
BcnI CCSGG 1 cut(s) 2937
BcoDI GTCTC 8 cut(s) 502, 1101, 1260, 1493, 1637, 1757, 2293, 3036
BfaI CTAG 5 cut(s) 362, 962, 977, 1647, 2339
BfmI CTRYAG 2 cut(s) 1834, 2613
BfrI CTTAAG 2 cut(s) 1324, 1354
BfuAI ACCTGC 2 cut(s) 326, 2620
BfuI GTATCC 1 cut(s) 1359
BglII AGATCT 3 cut(s) 1013, 1358, 1540
BisI GCNGC 4 cut(s) 20, 212, 441, 2437
BlpI GCTNAGC 1 cut(s) 1802
BlsI GCNGC 4 cut(s) 21, 213, 442, 2438
Bme1390I CCNGG 5 cut(s) 552, 1110, 2547, 2822, 2937
Bme18I GGWCC 3 cut(s) 548, 1288, 2933
BmeT110I CYCGRG 3 cut(s) 276, 1634, 1715
BmgT120I GGNCC 3 cut(s) 548, 1288, 2933
BmiI GGNNCC 2 cut(s) 239, 772
BmrFI CCNGG 5 cut(s) 552, 1110, 2547, 2822, 2937
BmrI ACTGGG 1 cut(s) 1353
BmsI GCATC 5 cut(s) 601, 1038, 1895, 2374, 2396
BmuI ACTGGG 1 cut(s) 1353
BpiI GAAGAC 3 cut(s) 1146, 1259, 1634
BpmI CTGGAG 1 cut(s) 2599
Bpu10I CCTNAGC 1 cut(s) 738
Bpu1102I GCTNAGC 1 cut(s) 1802
BpuEI CTTGAG 3 cut(s) 1550, 2291, 2930
BpuMI CCSGG 1 cut(s) 2937
BsaBI GATNNNNATC 1 cut(s) 498
BsaI GGTCTC 1 cut(s) 1493
BsaJI CCNNGG 4 cut(s) 241, 551, 1186, 1582
Bsc4I CCNNNNNNNGG 6 cut(s) 215, 989, 1192, 2111, 2686, 3074
Bse1I ACTGG 6 cut(s) 545, 885, 1348, 2582, 3084, 3097
Bse3DI GCAATG 1 cut(s) 31
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 4 cut(s) 552, 1110, 2547, 2822
BseDI CCNNGG 4 cut(s) 241, 551, 1186, 1582
BseGI GGATG 6 cut(s) 430, 889, 1556, 1910, 2021, 2695
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 6 cut(s) 215, 989, 1192, 2111, 2686, 3074
BseMI GCAATG 1 cut(s) 31
BseMII CTCAG 6 cut(s) 85, 752, 1817, 2132, 2553, 2933
BseNI ACTGG 6 cut(s) 545, 885, 1348, 2582, 3084, 3097
BseRI GAGGAG 1 cut(s) 1322
BseXI GCAGC 3 cut(s) 223, 427, 2423
BseYI CCCAGC 1 cut(s) 1074
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 2 cut(s) 360, 1302
BsiHKAI GWGCWC 2 cut(s) 1862, 2679
BsiHKCI CYCGRG 3 cut(s) 276, 1634, 1715
BsiSI CCGG 1 cut(s) 2936
BslI CCNNNNNNNGG 6 cut(s) 215, 989, 1192, 2111, 2686, 3074
BsmAI GTCTC 8 cut(s) 502, 1101, 1260, 1493, 1637, 1757, 2293, 3036
BsmI GAATGC 2 cut(s) 2238, 2465
BsnI GGCC 2 cut(s) 360, 1302
Bso31I GGTCTC 1 cut(s) 1493
BsoBI CYCGRG 3 cut(s) 276, 1634, 1715
Bsp1286I GDGCHC 2 cut(s) 1862, 2679
Bsp143I GATC 9 cut(s) 97, 237, 499, 943, 1013, 1358, 1540, 2033, 2833
Bsp1720I GCTNAGC 1 cut(s) 1802
Bsp19I CCATGG 1 cut(s) 241
BspACI CCGC 6 cut(s) 20, 175, 209, 250, 457, 1806
BspANI GGCC 2 cut(s) 360, 1302
BspCNI CTCAG 6 cut(s) 84, 751, 1818, 2133, 2554, 2934
BspFNI CGCG 1 cut(s) 177
BspHI TCATGA 1 cut(s) 1135
BspLI GGNNCC 2 cut(s) 239, 772
BspMAI CTGCAG 2 cut(s) 1838, 2617
BspMI ACCTGC 2 cut(s) 326, 2620
BspPI GGATC 6 cut(s) 232, 245, 507, 951, 2041, 2841
BspTI CTTAAG 2 cut(s) 1324, 1354
BspTNI GGTCTC 1 cut(s) 1493
BsrDI GCAATG 1 cut(s) 31
BsrI ACTGG 6 cut(s) 545, 885, 1348, 2582, 3084, 3097
BssECI CCNNGG 4 cut(s) 241, 551, 1186, 1582
BssMI GATC 9 cut(s) 97, 237, 499, 943, 1013, 1358, 1540, 2033, 2833
BssNAI GTATAC 1 cut(s) 132
BssSI CACGAG 2 cut(s) 758, 2859
BssT1I CCWWGG 3 cut(s) 241, 1186, 1582
Bst1107I GTATAC 1 cut(s) 132
Bst2BI CACGAG 2 cut(s) 758, 2859
Bst2UI CCWGG 4 cut(s) 552, 1110, 2547, 2822
Bst4CI ACNGT 6 cut(s) 641, 1044, 1095, 1952, 2630, 2897
Bst6I CTCTTC 2 cut(s) 1154, 2758
BstAFI CTTAAG 2 cut(s) 1324, 1354
BstAPI GCANNNNNTGC 3 cut(s) 1057, 2986, 3105
BstC8I GCNNGC 6 cut(s) 248, 919, 974, 1516, 2793, 3110
BstDSI CCRYGG 1 cut(s) 241
BstEII GGTNACC 1 cut(s) 2203
BstF5I GGATG 6 cut(s) 430, 889, 1556, 1910, 2021, 2695
BstFNI CGCG 1 cut(s) 177
BstKTI GATC 9 cut(s) 100, 240, 502, 946, 1016, 1361, 1543, 2036, 2836
BstMAI GTCTC 8 cut(s) 502, 1101, 1260, 1493, 1637, 1757, 2293, 3036
BstMBI GATC 9 cut(s) 97, 237, 499, 943, 1013, 1358, 1540, 2033, 2833
BstMWI GCNNNNNNNGC 6 cut(s) 1057, 2266, 2654, 2986, 3096, 3105
BstNI CCWGG 4 cut(s) 552, 1110, 2547, 2822
BstNSI RCATGY 4 cut(s) 250, 2496, 2500, 2987
BstPI GGTNACC 1 cut(s) 2203
BstSCI CCNGG 5 cut(s) 550, 1108, 2545, 2820, 2935
BstSFI CTRYAG 2 cut(s) 1834, 2613
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 3 cut(s) 223, 427, 2423
BstV2I GAAGAC 3 cut(s) 1146, 1259, 1634
BstX2I RGATCY 5 cut(s) 237, 1013, 1358, 1540, 2033
BstYI RGATCY 5 cut(s) 237, 1013, 1358, 1540, 2033
BstZ17I GTATAC 1 cut(s) 132
BsuI GTATCC 1 cut(s) 1359
BsuRI GGCC 2 cut(s) 360, 1302
BtgI CCRYGG 1 cut(s) 241
BtsCI GGATG 6 cut(s) 430, 889, 1556, 1910, 2021, 2695
BtsI GCAGTG 1 cut(s) 3028
BtsIMutI CAGTG 8 cut(s) 792, 1752, 2366, 2575, 2812, 3028, 3090, 3091
BveI ACCTGC 2 cut(s) 326, 2620
Cac8I GCNNGC 6 cut(s) 248, 919, 974, 1516, 2793, 3110
CaiI CAGNNNCTG 2 cut(s) 440, 2577
CciI TCATGA 1 cut(s) 1135
Cfr13I GGNCC 3 cut(s) 548, 1288, 2933
CseI GACGC 2 cut(s) 166, 2761
Csp6I GTAC 6 cut(s) 709, 2361, 2603, 2666, 2779, 2877
CviQI GTAC 6 cut(s) 709, 2361, 2603, 2666, 2779, 2877
DpnI GATC 9 cut(s) 99, 239, 501, 945, 1015, 1360, 1542, 2035, 2835
DpnII GATC 9 cut(s) 97, 237, 499, 943, 1013, 1358, 1540, 2033, 2833
Eam1104I CTCTTC 2 cut(s) 1154, 2758
EarI CTCTTC 2 cut(s) 1154, 2758
Eco130I CCWWGG 3 cut(s) 241, 1186, 1582
Eco147I AGGCCT 1 cut(s) 1302
Eco31I GGTCTC 1 cut(s) 1493
Eco47I GGWCC 3 cut(s) 548, 1288, 2933
Eco57I CTGAAG 3 cut(s) 1178, 2229, 2788
Eco88I CYCGRG 3 cut(s) 276, 1634, 1715
Eco91I GGTNACC 1 cut(s) 2203
EcoO65I GGTNACC 1 cut(s) 2203
EcoRI GAATTC 1 cut(s) 2708
EcoRII CCWGG 4 cut(s) 550, 1108, 2545, 2820
EcoT14I CCWWGG 3 cut(s) 241, 1186, 1582
EcoT22I ATGCAT 3 cut(s) 1053, 2389, 2498
ErhI CCWWGG 3 cut(s) 241, 1186, 1582
FalI AAGNNNNNCTT 4 cut(s) 366, 398, 2033, 2065
FauI CCCGC 2 cut(s) 182, 243
FblI GTMKAC 2 cut(s) 131, 2335
Fnu4HI GCNGC 4 cut(s) 20, 212, 441, 2437
FokI GGATG 6 cut(s) 437, 896, 1563, 1917, 2028, 2702
Fsp4HI GCNGC 4 cut(s) 20, 212, 441, 2437
FspBI CTAG 5 cut(s) 362, 962, 977, 1647, 2339
GluI GCNGC 4 cut(s) 20, 212, 441, 2437
GsaI CCCAGC 1 cut(s) 1078
GsuI CTGGAG 1 cut(s) 2599
HaeIII GGCC 2 cut(s) 360, 1302
HapII CCGG 1 cut(s) 2936
HgaI GACGC 2 cut(s) 166, 2761
HincII GTYRAC 4 cut(s) 148, 628, 1206, 1968
HindII GTYRAC 4 cut(s) 148, 628, 1206, 1968
HindIII AAGCTT 2 cut(s) 1598, 2267
HpaI GTTAAC 1 cut(s) 628
HpaII CCGG 1 cut(s) 2936
HphI GGTGA 3 cut(s) 2171, 2215, 2959
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 9 cut(s) 664, 1301, 1429, 1541, 2064, 2156, 2253, 2330, 2944
HpyCH4III ACNGT 6 cut(s) 641, 1044, 1095, 1952, 2630, 2897
HpyCH4IV ACGT 4 cut(s) 1117, 1738, 1977, 1999
HpyF10VI GCNNNNNNNGC 6 cut(s) 1057, 2266, 2654, 2986, 3096, 3105
HpySE526I ACGT 4 cut(s) 1117, 1738, 1977, 1999
KspAI GTTAAC 1 cut(s) 628
Kzo9I GATC 9 cut(s) 97, 237, 499, 943, 1013, 1358, 1540, 2033, 2833
LmnI GCTCC 2 cut(s) 437, 2165
Lsp1109I GCAGC 3 cut(s) 223, 427, 2423
LweI GCATC 5 cut(s) 601, 1038, 1895, 2374, 2396
MaeI CTAG 5 cut(s) 362, 962, 977, 1647, 2339
MaeII ACGT 4 cut(s) 1117, 1738, 1977, 1999
MaeIII GTNAC 5 cut(s) 27, 949, 2186, 2203, 2891
MalI GATC 9 cut(s) 99, 239, 501, 945, 1015, 1360, 1542, 2035, 2835
MboI GATC 9 cut(s) 97, 237, 499, 943, 1013, 1358, 1540, 2033, 2833
MfeI CAATTG 3 cut(s) 1791, 2431, 3069
MflI RGATCY 5 cut(s) 237, 1013, 1358, 1540, 2033
MhlI GDGCHC 2 cut(s) 1862, 2679
MlyI GAGTC 4 cut(s) 1323, 1648, 2837, 2970
MmeI TCCRAC 2 cut(s) 1705, 2811
Mph1103I ATGCAT 3 cut(s) 1053, 2389, 2498
MroXI GAANNNNTTC 6 cut(s) 169, 381, 651, 1083, 1177, 2529
MslI CAYNNNNRTG 4 cut(s) 164, 854, 1196, 2016
MspCI CTTAAG 2 cut(s) 1324, 1354
MspI CCGG 1 cut(s) 2936
MspR9I CCNGG 5 cut(s) 552, 1110, 2547, 2822, 2937
MunI CAATTG 3 cut(s) 1791, 2431, 3069
Mva1269I GAATGC 2 cut(s) 2238, 2465
MvaI CCWGG 4 cut(s) 552, 1110, 2547, 2822
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 6 cut(s) 1057, 2266, 2654, 2986, 3096, 3105
NciI CCSGG 1 cut(s) 2937
NcoI CCATGG 1 cut(s) 241
NdeII GATC 9 cut(s) 97, 237, 499, 943, 1013, 1358, 1540, 2033, 2833
NlaIV GGNNCC 2 cut(s) 239, 772
NmuCI GTSAC 2 cut(s) 949, 2203
NsiI ATGCAT 3 cut(s) 1053, 2389, 2498
NspI RCATGY 4 cut(s) 250, 2496, 2500, 2987
PaeI GCATGC 1 cut(s) 250
PaeR7I CTCGAG 2 cut(s) 276, 1634
PagI TCATGA 1 cut(s) 1135
PceI AGGCCT 1 cut(s) 1302
PciI ACATGT 1 cut(s) 2983
PctI GAATGC 2 cut(s) 2238, 2465
PdmI GAANNNNTTC 6 cut(s) 169, 381, 651, 1083, 1177, 2529
PflFI GACNNNGTC 2 cut(s) 983, 2937
PflMI CCANNNNNTGG 2 cut(s) 1192, 2686
PfoI TCCNGGA 1 cut(s) 2545
PkrI GCNGC 4 cut(s) 21, 213, 442, 2438
PleI GAGTC 4 cut(s) 1323, 1647, 2836, 2969
PpsI GAGTC 4 cut(s) 1323, 1647, 2836, 2969
PscI ACATGT 1 cut(s) 2983
PshBI ATTAAT 1 cut(s) 1679
PsiI TTATAA 2 cut(s) 927, 1985
Psp1406I AACGTT 1 cut(s) 1738
Psp6I CCWGG 4 cut(s) 550, 1108, 2545, 2820
PspEI GGTNACC 1 cut(s) 2203
PspFI CCCAGC 1 cut(s) 1074
PspGI CCWGG 4 cut(s) 550, 1108, 2545, 2820
PspN4I GGNNCC 2 cut(s) 239, 772
PspPI GGNCC 3 cut(s) 548, 1288, 2933
PspXI VCTCGAGB 1 cut(s) 276
PstI CTGCAG 2 cut(s) 1838, 2617
PstNI CAGNNNCTG 2 cut(s) 440, 2577
PsuI RGATCY 5 cut(s) 237, 1013, 1358, 1540, 2033
PsyI GACNNNGTC 2 cut(s) 983, 2937
RsaI GTAC 6 cut(s) 710, 2362, 2604, 2667, 2780, 2878
RsaNI GTAC 6 cut(s) 709, 2361, 2603, 2666, 2779, 2877
RseI CAYNNNNRTG 4 cut(s) 164, 854, 1196, 2016
SatI GCNGC 4 cut(s) 20, 212, 441, 2437
Sau3AI GATC 9 cut(s) 97, 237, 499, 943, 1013, 1358, 1540, 2033, 2833
Sau96I GGNCC 3 cut(s) 548, 1288, 2933
SchI GAGTC 4 cut(s) 1323, 1648, 2837, 2970
ScrFI CCNGG 5 cut(s) 552, 1110, 2547, 2822, 2937
SduI GDGCHC 2 cut(s) 1862, 2679
SfaNI GCATC 5 cut(s) 601, 1038, 1895, 2374, 2396
SfcI CTRYAG 2 cut(s) 1834, 2613
Sfr274I CTCGAG 2 cut(s) 276, 1634
SinI GGWCC 3 cut(s) 548, 1288, 2933
SlaI CTCGAG 2 cut(s) 276, 1634
SmiMI CAYNNNNRTG 4 cut(s) 164, 854, 1196, 2016
SmlI CTYRAG 7 cut(s) 276, 1324, 1354, 1529, 1634, 2270, 2909
SmoI CTYRAG 7 cut(s) 276, 1324, 1354, 1529, 1634, 2270, 2909
SphI GCATGC 1 cut(s) 250
SseBI AGGCCT 1 cut(s) 1302
SsiI CCGC 6 cut(s) 20, 175, 209, 250, 457, 1806
SspI AATATT 2 cut(s) 9, 903
SspMI CTAG 5 cut(s) 362, 962, 977, 1647, 2339
StuI AGGCCT 1 cut(s) 1302
StyD4I CCNGG 5 cut(s) 550, 1108, 2545, 2820, 2935
StyI CCWWGG 3 cut(s) 241, 1186, 1582
TaaI ACNGT 6 cut(s) 641, 1044, 1095, 1952, 2630, 2897
TaiI ACGT 4 cut(s) 1120, 1741, 1980, 2002
TaqI TCGA 7 cut(s) 180, 277, 1046, 1335, 1635, 2632, 2757
TaqII GACCGA 1 cut(s) 327
TatI WGTACW 2 cut(s) 2360, 2665
TauI GCSGC 1 cut(s) 22
TscAI CASTG 8 cut(s) 799, 1752, 2373, 2582, 2812, 3028, 3091, 3097
TseFI GTSAC 2 cut(s) 949, 2203
TseI GCWGC 3 cut(s) 211, 440, 2436
Tsp45I GTSAC 2 cut(s) 949, 2203
TspGWI ACGGA 2 cut(s) 115, 2954
TspRI CASTG 8 cut(s) 799, 1752, 2373, 2582, 2812, 3028, 3091, 3097
Tth111I GACNNNGTC 2 cut(s) 983, 2937
Van91I CCANNNNNTGG 2 cut(s) 1192, 2686
Vha464I CTTAAG 2 cut(s) 1324, 1354
VpaK11BI GGWCC 3 cut(s) 548, 1288, 2933
VspI ATTAAT 1 cut(s) 1679
XapI RAATTY 9 cut(s) 64, 1249, 1373, 1447, 1558, 2180, 2392, 2708, 2813
XceI RCATGY 4 cut(s) 250, 2496, 2500, 2987
XcmI CCANNNNNNNNNTGG 3 cut(s) 397, 1580, 2582
XhoI CTCGAG 2 cut(s) 276, 1634
XmiI GTMKAC 2 cut(s) 131, 2335
XmnI GAANNNNTTC 6 cut(s) 169, 381, 651, 1083, 1177, 2529
XspI CTAG 5 cut(s) 362, 962, 977, 1647, 2339
Zsp2I ATGCAT 3 cut(s) 1053, 2389, 2498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.