Rroxscaffold_180G00433550

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000180
OG0000105
Physical Location & Seq
Reverse (-)
605532 .. 612415
6884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_180G00433550.1

Sequence Viewer

Length: 1572 bp
ATGATTTGCTACAAGAAAATGGGCTGGGAAATAGTTCGTCGACAGTCTCCTAAAGAGTCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCCACCATGTGTTGAAGAAAAATAAGGGAACAGAAGCAATCCAAGGGATGGTAATGGAGTTGCCTAAATTAGAAGTGGCTCATTGGAATCCAGAAGCCTTCTCAAATTTGTCTGAACTTAATCTTCTCCATATTCGTAATGTGGACCTTCCTAAAGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTGGAATGGCCTGGGTGTCCGCTAAGATCTCTCCCACAAAAGTTCGAATCAGATGAACTTATTGAACTTAACTTGTGTCACAGCAATATTGAACATCTTTGGGAGGGAGAAAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGACCTCGCGGGCGTTCAGAATCTTGAGAATTTAGATCTTGAAGGATGTAAGAGTTTGGTAAGAATCCATCAATCCCTCGGATTTCTGAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTTGAGAATCTGCCAGGTAGAATTGAAATGGAATCTCTTCAAACATTAATTCTGTCTAACTGCTCAAAAATTAAAAAGATTCCCGAGTTTGATGGAAATATGGAGCGTCTGTCTGTGCTTGATCTCGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGGACGCCTGAGTGGCCTTTTGTCATTGAATCTAAGTAACTGCACAAATCTTGTCCGTCTTCCAAGCACCATCAATAATTTGAAGTTTGTTAAAAATCTTAATCTTTCTGGATGCATGAAACTTGGCAAACATCAGGAAAGCGTGGGGGAGAGGGAATGTTATGAGGAAAATGATGTGAATAGTGGGTCTGCAATAGAAATGTCATCTACCTATGATCTCATAAAGCATGTGAGAGGTTCATTCGTTCATGGATGCGAAGTGAATAGTTTCCGCTTGCCTATATGGGGTCTCTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGCGAAGGAGCATTTGCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGTCTTCCTTCAGGCATTGGATCGCTTTCTAAGCTTGAGAACTTTAACTTGGAAAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTAGCCTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTGAACAAATCATATTTCAATTTCATCAATTGCGTCAATCTAAATGGCAATCAGGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTGATAAGAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCGAAGTGTTGGGCGTTCATTAAGGGCACGTTTACTTACTTGGAATGGAATGGAATGTAATGGAATGATTACGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

523

Amino Acids

59.15

Weight (kDa)

6.52

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 185 - 286 4.2e-10 Leucine-rich repeat region
LRR_13 PF23286 205 - 290 2.4e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 142
AccI GTMKAC 1 cut(s) 40
AccII CGCG 1 cut(s) 464
AciI CCGC 3 cut(s) 302, 464, 1015
AclWI GGATC 1 cut(s) 1180
AcsI RAATTY 5 cut(s) 199, 397, 484, 1097, 1309
AcuI CTGAAG 2 cut(s) 1146, 1424
AcyI GRCGYC 1 cut(s) 748
AdeI CACNNNGTG 1 cut(s) 103
AfaI GTAC 1 cut(s) 1279
AfiI CCNNNNNNNGG 2 cut(s) 142, 1028
AjnI CCWGG 4 cut(s) 282, 292, 595, 1462
AjuI GAANNNNNNNTTGG 2 cut(s) 1184, 1216
AluBI AGCT 2 cut(s) 550, 1186
AluI AGCT 2 cut(s) 550, 1186
Alw26I GTCTC 7 cut(s) 51, 87, 271, 443, 707, 1037, 1210
AlwI GGATC 1 cut(s) 1180
Ama87I CYCGRG 1 cut(s) 665
AoxI GGCC 3 cut(s) 250, 290, 757
ApoI RAATTY 5 cut(s) 199, 397, 484, 1097, 1309
AseI ATTAAT 1 cut(s) 629
Asp700I GAANNNNTTC 5 cut(s) 33, 618, 1010, 1409, 1446
AspS9I GGNCC 1 cut(s) 238
AsuHPI GGTGA 1 cut(s) 1132
AsuII TTCGAA 1 cut(s) 327
AvaI CYCGRG 1 cut(s) 665
AvaII GGWCC 1 cut(s) 238
BaeGI GKGCMC 1 cut(s) 1522
BbsI GAAGAC 4 cut(s) 96, 794, 1148, 1412
BccI CCATC 7 cut(s) 136, 531, 668, 821, 1249, 1265, 1500
BcgI CGANNNNNNTGC 2 cut(s) 698, 732
BciT130I CCWGG 4 cut(s) 284, 294, 597, 1464
BcoDI GTCTC 7 cut(s) 51, 87, 271, 443, 707, 1037, 1210
BfaI CTAG 1 cut(s) 1256
BglI GCCNNNNNGGC 1 cut(s) 756
BglII AGATCT 2 cut(s) 308, 490
Bme1390I CCNGG 4 cut(s) 284, 294, 597, 1464
Bme18I GGWCC 1 cut(s) 238
BmeT110I CYCGRG 1 cut(s) 665
BmgT120I GGNCC 1 cut(s) 238
BmrFI CCNGG 4 cut(s) 284, 294, 597, 1464
BmsI GCATC 5 cut(s) 845, 986, 1291, 1313, 1367
BpiI GAAGAC 4 cut(s) 96, 794, 1148, 1412
Bpu14I TTCGAA 1 cut(s) 327
BpuEI CTTGAG 4 cut(s) 295, 500, 605, 1208
BsaHI GRCGYC 1 cut(s) 748
BsaI GGTCTC 3 cut(s) 87, 443, 1037
BsaJI CCNNGG 3 cut(s) 136, 293, 532
BsaXI ACNNNNNCTCC 2 cut(s) 677, 707
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 1028
BseBI CCWGG 4 cut(s) 284, 294, 597, 1464
BseDI CCNNGG 3 cut(s) 136, 293, 532
BseGI GGATG 6 cut(s) 93, 147, 506, 860, 1001, 1382
BseLI CCNNNNNNNGG 2 cut(s) 142, 1028
BseMII CTCAG 3 cut(s) 743, 1049, 1470
BseSI GKGCMC 1 cut(s) 1522
BseYI CCCAGC 1 cut(s) 24
BsgI GTGCAG 1 cut(s) 769
Bsh1236I CGCG 1 cut(s) 464
BshFI GGCC 3 cut(s) 252, 292, 759
BsiHKCI CYCGRG 1 cut(s) 665
BslI CCNNNNNNNGG 2 cut(s) 142, 1028
BsmAI GTCTC 7 cut(s) 51, 87, 271, 443, 707, 1037, 1210
BsnI GGCC 3 cut(s) 252, 292, 759
Bso31I GGTCTC 3 cut(s) 87, 443, 1037
BsoBI CYCGRG 1 cut(s) 665
Bsp119I TTCGAA 1 cut(s) 327
Bsp1286I GDGCHC 1 cut(s) 1522
Bsp143I GATC 5 cut(s) 308, 490, 703, 958, 1172
BspACI CCGC 3 cut(s) 302, 464, 1015
BspANI GGCC 3 cut(s) 252, 292, 759
BspCNI CTCAG 3 cut(s) 744, 1050, 1471
BspFNI CGCG 1 cut(s) 464
BspHI TCATGA 1 cut(s) 85
BspPI GGATC 1 cut(s) 1180
BspT104I TTCGAA 1 cut(s) 327
BspTNI GGTCTC 3 cut(s) 87, 443, 1037
BssECI CCNNGG 3 cut(s) 136, 293, 532
BssMI GATC 5 cut(s) 308, 490, 703, 958, 1172
BssNI GRCGYC 1 cut(s) 748
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 4 cut(s) 284, 294, 597, 1464
Bst4CI ACNGT 1 cut(s) 45
Bst6I CTCTTC 1 cut(s) 624
BstACI GRCGYC 1 cut(s) 748
BstBI TTCGAA 1 cut(s) 327
BstC8I GCNNGC 2 cut(s) 466, 1019
BstDEI CTNAG 7 cut(s) 305, 752, 776, 1058, 1133, 1182, 1479
BstEII GGTNACC 1 cut(s) 1120
BstF5I GGATG 6 cut(s) 93, 147, 506, 860, 1001, 1382
BstFNI CGCG 1 cut(s) 464
BstKTI GATC 5 cut(s) 311, 493, 706, 961, 1175
BstMAI GTCTC 7 cut(s) 51, 87, 271, 443, 707, 1037, 1210
BstMBI GATC 5 cut(s) 308, 490, 703, 958, 1172
BstMWI GCNNNNNNNGC 3 cut(s) 756, 1074, 1183
BstNI CCWGG 4 cut(s) 284, 294, 597, 1464
BstNSI RCATGY 1 cut(s) 974
BstPI GGTNACC 1 cut(s) 1120
BstSCI CCNGG 4 cut(s) 282, 292, 595, 1462
BstSLI GKGCMC 1 cut(s) 1522
BstUI CGCG 1 cut(s) 464
BstV2I GAAGAC 4 cut(s) 96, 794, 1148, 1412
BstX2I RGATCY 2 cut(s) 308, 490
BstYI RGATCY 2 cut(s) 308, 490
BsuRI GGCC 3 cut(s) 252, 292, 759
BtsCI GGATG 6 cut(s) 93, 147, 506, 860, 1001, 1382
BtsIMutI CAGTG 1 cut(s) 1283
Cac8I GCNNGC 2 cut(s) 466, 1019
CciI TCATGA 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 238
CseI GACGC 3 cut(s) 677, 756, 1342
Csp6I GTAC 1 cut(s) 1278
CviAII CATG 6 cut(s) 86, 101, 859, 971, 992, 1414
CviQI GTAC 1 cut(s) 1278
DdeI CTNAG 7 cut(s) 305, 752, 776, 1058, 1133, 1182, 1479
DpnI GATC 5 cut(s) 310, 492, 705, 960, 1174
DpnII GATC 5 cut(s) 308, 490, 703, 958, 1172
DraIII CACNNNGTG 1 cut(s) 103
Eam1104I CTCTTC 1 cut(s) 624
EarI CTCTTC 1 cut(s) 624
Eco130I CCWWGG 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 252
Eco31I GGTCTC 3 cut(s) 87, 443, 1037
Eco47I GGWCC 1 cut(s) 238
Eco57I CTGAAG 2 cut(s) 1146, 1424
Eco88I CYCGRG 1 cut(s) 665
Eco91I GGTNACC 1 cut(s) 1120
EcoO65I GGTNACC 1 cut(s) 1120
EcoRII CCWGG 4 cut(s) 282, 292, 595, 1462
EcoT14I CCWWGG 1 cut(s) 136
EcoT22I ATGCAT 2 cut(s) 860, 1306
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 6 cut(s) 89, 104, 862, 974, 995, 1417
FatI CATG 6 cut(s) 85, 100, 858, 970, 991, 1413
FauI CCCGC 1 cut(s) 457
FblI GTMKAC 1 cut(s) 40
FokI GGATG 6 cut(s) 80, 154, 513, 867, 1008, 1389
FspBI CTAG 1 cut(s) 1256
GsaI CCCAGC 1 cut(s) 28
HaeIII GGCC 3 cut(s) 252, 292, 759
HgaI GACGC 3 cut(s) 677, 756, 1342
Hin1I GRCGYC 1 cut(s) 748
Hin1II CATG 6 cut(s) 89, 104, 862, 974, 995, 1417
HincII GTYRAC 1 cut(s) 41
HindII GTYRAC 1 cut(s) 41
HindIII AAGCTT 2 cut(s) 548, 1184
HphI GGTGA 1 cut(s) 1132
Hpy166II GTNNAC 3 cut(s) 41, 238, 1526
Hpy188I TCNGA 8 cut(s) 208, 334, 474, 536, 543, 1053, 1234, 1432
Hpy8I GTNNAC 3 cut(s) 41, 238, 1526
Hpy99I CGWCG 1 cut(s) 42
HpyAV CCTTC 6 cut(s) 202, 251, 491, 1073, 1170, 1247
HpyCH4III ACNGT 1 cut(s) 45
HpyCH4IV ACGT 2 cut(s) 67, 1522
HpyCH4V TGCA 8 cut(s) 576, 786, 858, 935, 1068, 1212, 1304, 1380
HpyF10VI GCNNNNNNNGC 3 cut(s) 756, 1074, 1183
HpyF3I CTNAG 7 cut(s) 305, 752, 776, 1058, 1133, 1182, 1479
HpySE526I ACGT 2 cut(s) 67, 1522
Hsp92I GRCGYC 1 cut(s) 748
Hsp92II CATG 6 cut(s) 89, 104, 862, 974, 995, 1417
Kzo9I GATC 5 cut(s) 308, 490, 703, 958, 1172
LmnI GCTCC 2 cut(s) 685, 1082
LweI GCATC 5 cut(s) 845, 986, 1291, 1313, 1367
MaeI CTAG 1 cut(s) 1256
MaeII ACGT 2 cut(s) 67, 1522
MaeIII GTNAC 4 cut(s) 359, 779, 1103, 1120
MalI GATC 5 cut(s) 310, 492, 705, 960, 1174
MboI GATC 5 cut(s) 308, 490, 703, 958, 1172
MboII GAAGA 7 cut(s) 101, 121, 209, 611, 794, 1148, 1417
MfeI CAATTG 2 cut(s) 741, 1348
MflI RGATCY 2 cut(s) 308, 490
MhlI GDGCHC 1 cut(s) 1522
MlyI GAGTC 2 cut(s) 65, 273
Mph1103I ATGCAT 2 cut(s) 860, 1306
MroXI GAANNNNTTC 5 cut(s) 33, 618, 1010, 1409, 1446
MslI CAYNNNNRTG 1 cut(s) 1497
MspR9I CCNGG 4 cut(s) 284, 294, 597, 1464
MunI CAATTG 2 cut(s) 741, 1348
MvaI CCWGG 4 cut(s) 284, 294, 597, 1464
MvnI CGCG 1 cut(s) 464
MwoI GCNNNNNNNGC 3 cut(s) 756, 1074, 1183
NdeII GATC 5 cut(s) 308, 490, 703, 958, 1172
NlaIII CATG 6 cut(s) 89, 104, 862, 974, 995, 1417
NmuCI GTSAC 2 cut(s) 359, 1120
NsiI ATGCAT 2 cut(s) 860, 1306
NspI RCATGY 1 cut(s) 974
NspV TTCGAA 1 cut(s) 327
PagI TCATGA 1 cut(s) 85
PceI AGGCCT 1 cut(s) 252
PdmI GAANNNNTTC 5 cut(s) 33, 618, 1010, 1409, 1446
PflMI CCANNNNNTGG 1 cut(s) 142
PfoI TCCNGGA 2 cut(s) 282, 1462
PleI GAGTC 2 cut(s) 64, 273
PpsI GAGTC 2 cut(s) 64, 273
PshBI ATTAAT 1 cut(s) 629
Psp6I CCWGG 4 cut(s) 282, 292, 595, 1462
PspEI GGTNACC 1 cut(s) 1120
PspFI CCCAGC 1 cut(s) 24
PspGI CCWGG 4 cut(s) 282, 292, 595, 1462
PspPI GGNCC 1 cut(s) 238
PsuI RGATCY 2 cut(s) 308, 490
RsaI GTAC 1 cut(s) 1279
RsaNI GTAC 1 cut(s) 1278
RseI CAYNNNNRTG 1 cut(s) 1497
SalI GTCGAC 1 cut(s) 39
Sau3AI GATC 5 cut(s) 308, 490, 703, 958, 1172
Sau96I GGNCC 1 cut(s) 238
SchI GAGTC 2 cut(s) 65, 273
ScrFI CCNGG 4 cut(s) 284, 294, 597, 1464
SduI GDGCHC 1 cut(s) 1522
SfaNI GCATC 5 cut(s) 845, 986, 1291, 1313, 1367
SfuI TTCGAA 1 cut(s) 327
SinI GGWCC 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 1497
SmlI CTYRAG 4 cut(s) 274, 479, 584, 1187
SmoI CTYRAG 4 cut(s) 274, 479, 584, 1187
SseBI AGGCCT 1 cut(s) 252
SsiI CCGC 3 cut(s) 302, 464, 1015
SspI AATATT 2 cut(s) 370, 1474
SspMI CTAG 1 cut(s) 1256
StuI AGGCCT 1 cut(s) 252
StyD4I CCNGG 4 cut(s) 282, 292, 595, 1462
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 45
TaiI ACGT 2 cut(s) 70, 1525
TaqI TCGA 4 cut(s) 40, 327, 708, 1495
TatI WGTACW 1 cut(s) 1277
TscAI CASTG 1 cut(s) 1290
TseFI GTSAC 2 cut(s) 359, 1120
Tsp45I GTSAC 2 cut(s) 359, 1120
TspDTI ATGAA 9 cut(s) 102, 351, 406, 875, 972, 980, 1333, 1402, 1500
TspGWI ACGGA 1 cut(s) 788
TspRI CASTG 1 cut(s) 1290
Van91I CCANNNNNTGG 1 cut(s) 142
VpaK11BI GGWCC 1 cut(s) 238
VspI ATTAAT 1 cut(s) 629
XapI RAATTY 5 cut(s) 199, 397, 484, 1097, 1309
XceI RCATGY 1 cut(s) 974
XcmI CCANNNNNNNNNTGG 1 cut(s) 1499
XmiI GTMKAC 1 cut(s) 40
XmnI GAANNNNTTC 5 cut(s) 33, 618, 1010, 1409, 1446
XspI CTAG 1 cut(s) 1256
Zsp2I ATGCAT 2 cut(s) 860, 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.