RLG00000016540

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000105
Physical Location & Seq
Forward (+)
7460142 .. 7468130
7989 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016540

Sequence Viewer

Length: 3735 bp
ATGGACGACGACTATTACTACGACTGTCGTTCACATGAAGCTAGTATTGATGATGAATGCAGCAGCATTGAGGACATGGACTATCAGGATGAAGATGGTATTTATGATTATCGCCCAAGCAATTGTAAAGCCAACTTGTTTCAACTCTGCACTCGTGATAAACTTGAGCTTAGTACTAGTATCACTCATGATGAGTCCTCTCGGCGGTCAACTTCTTCTCCCCGTCCTTCCCAATCGTACAGATACGATGTGTTTATAAGTTTCGGAAGGGAACACACCCGCTCGAGTTTTGTGAGCCATTTATATGCTGCATTAGATCTGAGTGGAATAGTCCCCTTTAAAGATTACGAAAGGCTTGAGAGAGGGAAATCCATTTCTGATCCGGAACTAGTTTTAAAAGCTATTGAGGACTCAAAAGTTGCCCTTGTCATTCTTTCACCTAACTATGGTTCTTCAACATGGTGCTTGGATGAGCTGCTCAAGATTCTTCAATGCAGAGAAGATATGGGGCTAGTAGTGATGCCAGTGTTTTACCACCTGCAAGCTTCTTTTATTCGGAAGAACTACTTATCGGAGAAAAACAATGAGTGGAAATATTTGAACGCCTTTACTGAACATGAAGAAATTTATATGGAGGAAGGGAGCGATGTCAAGAAATATACCACTGCTTTGAAAAGCTTAGCCAATCTTTCCGGCTTCGTTCTTCAAGATGGAATGGACGAGGCAAATGTCATCAAAAGCATCATTCGTGCAACAGCAAAACATGCATGTTCAAATTTTGACAAGGGCTTGGTAGGAACAATGAATAATGTATTACATAGTTACATTACTGATGACTCCTCTCGGCCGTCAACTTCCTCTCCCCGTCCTCATCAAAATCATATATACGACGTCTTTTTGAGTTTCAGAGGTGTAGACACCCGCTCCAATTTTGTGAGCCACTTATATTCTGCATTAGAGCGGAATGGAATTTTTACCTTCAAGGATGATAAAAGGCTTGAGAGAGGAAAATCCATTTCGCCAGAACTTTTAAAAGCAATTGAGGACTCAAAAGTTGCTATTGTCATTCTTTCACCAAACTATGGTACTTCAACGTGGTGCTTGGATGAGCTGTTGAAGATTCTTCAGTGCAGAGAAGATCTGGGACAAGTCGTGATGCCAGTCTTTTACCAACTGCAGCCTTCTGTTGTACGAAATAACTTTTTAAGGCAGAAGAATGAGGGAAAGTTTTGGCAGGTCTTTACTAGACATGAAGAAGAGGAACCGGGCAAATTAAAGCAATGGAGCGCTGCTTTGACAAACTTGGCCAATCTTTCAGGCTTCGTTCTTCAAGAGGGCATGGACGAGGCAGATGTCATCAGAAGTATTGTTCGTGCGACGGCAAAACATGTGTCGTATACATGCTCAAATGCTGGCAAGGACTTCGTAGGAATGGATCCCCGCATTGATGATTTATTACATAATTACATATGTCCATGGTCTGATGCCGTGCGTATTATAGGGGTTCATGGGATGCGGGGAATAGGTAAGACAACACTTGCCCGAGCTGTTTATGATCAAATTTGTGAAAGTTTTGACCATAGATGCTTTCTTTCCGATGTTAGAGAAATGTCTAAAGTCAATGGCCTAGTTTCTTTGCAAAAAAAACTTCTTTCCGGGTTCCTCATGGATAAAGTTGAAAATATTGAGGATGAATATAACGGAGCAGCTATAATAGAAAAATGCATGTGCAGAATAAGAGTGCTTCTTGTTCTTGATGATGTGGATCAACTGTCACAATTAAAAAAACTAGCTGGCCATAGCCACTGGTTCGGTCCAGGCAGTAGAATAATCATTACAAGCACAGATATTCATCTGCTGAACACGCATGGTGTTGATGCTGTCTATCAGGCTACTGTGTTAGATCCTAATGAAGCACTTCAACTTATGAGTTTAAAGGCTTTTAAGAAAATCCCCCCGCCTGAAAATTATTTGGAGCTATGCCACAGCATTTTAACCTATGCTCATGGACTTCCTTTAGCTCTTGTGGTTATGGGTTGCTTTCTGTATGGTAAAAGCACCAGTGAATGGAGAAGTGCAATAGATATGCTCAAGAAAACACCTGAAAGAAGTATCACTGATGTGCTTCAGATAAGTTTTGACGGACTAAGGGAAATAGAAAAAGAAGTATTCCTACATATTGCTTGTTTTTATAAGGGCAAGGATAGGAATCGTGTGACAGAAATACTAGACTATTGTCAGCTTCACCCTGACATTAATTTAAAAGTTCTTGTTGATAAATCTCTCATTACTATTTCAAACAACGAAATCTGCATGCATGATTTGCTACAAGAAATGGGAAGGGAAATAGTTCGTCAAGAGTCCCCTAAAGAGCCAGGCAAACGTAGTAGATTATGGTCACATGAAGACATACACAACGTGCTGAAGAAAAATGAGTGTTTGGACAAGTTGAAGCTCATCAAACTCTGCCATTCCCATAACATTGTCAAAACGCCAGACTTCACGGGCGTTCAAAATCTTGAGACATTAGATCTTGAAGGATGCATAAATCTGTCACGAGTTCATCAATCCCTTGGATTTCTCAAGAGGCTTGTTGTACTGAATCTTAAAGACTGCAAAAGTCTTGAGAGCCTGCCAGGTAAAATCGAATCACAGTCTCTTGAAACTTTTATTCTTTCTGGCTGCTCAAAAGTTAAGAAGATCCCTGAGTTTATTGAAAATATGAAACACTTGTCGACGCTTTATCTCGACAAGACTGCCATAAAAGAACTACCTTCCTCAATTGAACATCTCAGTGGCCTTGCTTCTTTGGATCTAAGCAACTGCAAAGACCTTGTTTCACTTCCGAGCACAATTCATTCGTTGAAGTCTCTTAAAAAGCTGAATCTTTCTGGATGCTTGAAACTTGGCCAAAGCCATGCCAGTGTAACAGAATGGAAGGATCTTTTAAGACATGGTTGTGAAGTGGTTTGGGAATATTTCTCATGGTCCTTGTCTGCTGTATGGATGCAAAGCCACGATACAGAACCAACTAGTATGCCGTTGTCTGTATCTGATCTGTGTAGCTCTACAGAGCAAAGAATGAACAGATCACTGAGCATGCGGTTGCCTTTATTTGGTCTTTGTAATTTAACAGATCTAAACCTAAGTAACTGCAATCTTGGTGACACAGCATTTGCCAACAACTTCGGCTGCTTTCCCTCTTTACTGGCATTAAATCTCAGTGGAAATAATTTTTCTAAGCTTCCCCCAGGCATCAGATCATGTTTGAAACTAGAGAAGATTAACTTGGAGAACTGCATGAGTCTTCAAGAGTTGTCAGGCCTTCCTTCAAATAGTACATTGGATATAAGAGCAGATGGTTGTTCTTCACTGGAAATGTTGCTTCATGCATTCAATTTTGATAGATTGAACAGATCATATTTGAATTTCATCAACTGCTTCAAAATAAAGGACAATCAAGGCTGCAATAGCATATCATTTGAAATGCTGAAGTTATTCCTTTGCCAGGGCATCTCTAATGCAAAGCAAACTTTTCAAGTTGTACTTCCTGGAAGGAAAATTCCCAGCTGGTTCAGCCATGAAAGGTCTGGAGTTTCGTTAAGTCTAAGGCGAAGTCCAGGTCAGACCCAAAAACTAATGACCAAGCTCCACAGGGGTTGTAGACAAGCTAAAGCAGGGAGCAGAGGTAAGCAAGCGAGTACTGAAAATGTCAAGGCACAAACAACCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1245

Amino Acids

140.68

Weight (kDa)

6.65

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 82 - 254 2e-35 TIR domain
TIR_2 PF13676 84 - 181 1.6e-10 TIR domain
TIR PF01582 296 - 467 4.1e-47 TIR domain
TIR_2 PF13676 298 - 395 5.4e-16 TIR domain
NB-ARC PF00931 495 - 650 2e-17 NB-ARC domain
WHD_ROQ1 PF23282 719 - 788 1.5e-21 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 858 - 966 1.2e-10 Leucine-rich repeat region
LRR_13 PF23286 886 - 973 2.8e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 257, 2193
AarI CACCTGC 1 cut(s) 546
AatII GACGTC 1 cut(s) 894
Acc36I ACCTGC 2 cut(s) 546, 1225
AccB7I CCANNNNNTGG 2 cut(s) 1082, 2067
AccBSI CCGCTC 3 cut(s) 282, 924, 961
AccI GTMKAC 4 cut(s) 915, 1397, 2735, 3664
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 8 cut(s) 205, 280, 922, 961, 1441, 1516, 1958, 3103
AclWI GGATC 8 cut(s) 374, 1430, 1443, 1773, 1898, 2695, 2820, 2949
AcoI YGGCCR 4 cut(s) 845, 1305, 1795, 2908
AcsI RAATTY 6 cut(s) 624, 775, 969, 1560, 3427, 3561
AcuI CTGAAG 4 cut(s) 1109, 2111, 2444, 3512
AcyI GRCGYC 1 cut(s) 891
AdeI CACNNNGTG 1 cut(s) 2419
AfaI GTAC 8 cut(s) 175, 239, 1087, 1191, 2598, 3340, 3546, 3703
AfeI AGCGCT 1 cut(s) 1288
AfiI CCNNNNNNNGG 6 cut(s) 204, 446, 1082, 2067, 3116, 3508
AflIII ACRYGT 1 cut(s) 1387
AhlI ACTAGT 3 cut(s) 176, 388, 3032
AjnI CCWGG 7 cut(s) 1816, 2374, 2635, 3250, 3507, 3550, 3619
AjuI GAANNNNNNNTTGG 2 cut(s) 3272, 3304
AleI CACNNNNGTG 1 cut(s) 2120
Alw21I GWGCWC 1 cut(s) 2852
Alw26I GTCTC 3 cut(s) 2516, 2661, 2874
AlwI GGATC 8 cut(s) 374, 1430, 1443, 1773, 1898, 2695, 2820, 2949
Ama87I CYCGRG 2 cut(s) 283, 1542
Aor13HI TCCGGA 1 cut(s) 382
Aor51HI AGCGCT 1 cut(s) 1288
AoxI GGCC 7 cut(s) 845, 1305, 1624, 1795, 2797, 2908, 3322
ApoI RAATTY 6 cut(s) 624, 775, 969, 1560, 3427, 3561
ArsI GACNNNNNNTTYG 6 cut(s) 1376, 1408, 3158, 3190, 3607, 3639
AseI ATTAAT 1 cut(s) 2256
Asp700I GAANNNNTTC 3 cut(s) 1917, 2349, 3497
AspLEI GCGC 1 cut(s) 1289
AspS9I GGNCC 2 cut(s) 1814, 2988
AsuC2I CCSGG 2 cut(s) 1266, 1657
AsuHPI GGTGA 4 cut(s) 429, 1065, 2237, 3176
AvaI CYCGRG 2 cut(s) 283, 1542
AvaII GGWCC 2 cut(s) 1814, 2988
BalI TGGCCA 3 cut(s) 1307, 1797, 2910
BamHI GGATCC 1 cut(s) 1435
BarI GAAGNNNNNNTAC 2 cut(s) 2157, 2189
BauI CACGAG 2 cut(s) 153, 2556
BbsI GAAGAC 2 cut(s) 2412, 3299
Bbv12I GWGCWC 1 cut(s) 2852
BccI CCATC 3 cut(s) 89, 704, 3353
BceAI ACGGC 4 cut(s) 832, 1395, 1472, 3025
BcgI CGANNNNNNTGC 4 cut(s) 1405, 1439, 2738, 2772
BciT130I CCWGG 7 cut(s) 1818, 2376, 2637, 3252, 3509, 3552, 3621
BclI TGATCA 1 cut(s) 1555
BcnI CCSGG 2 cut(s) 1266, 1657
BcoDI GTCTC 3 cut(s) 2516, 2661, 2874
BcuI ACTAGT 3 cut(s) 176, 388, 3032
BfmI CTRYAG 2 cut(s) 1175, 3069
BfoI RGCGCY 1 cut(s) 1290
BfuAI ACCTGC 2 cut(s) 546, 1225
BglII AGATCT 4 cut(s) 316, 1138, 2530, 3136
BlpI GCTNAGC 1 cut(s) 679
BmcAI AGTACT 2 cut(s) 175, 3703
Bme1390I CCNGG 9 cut(s) 1266, 1657, 1818, 2376, 2637, 3252, 3509, 3552, 3621
Bme18I GGWCC 2 cut(s) 1814, 2988
BmeT110I CYCGRG 2 cut(s) 283, 1542
BmgT120I GGNCC 2 cut(s) 1814, 2988
BmiI GGNNCC 3 cut(s) 1263, 1437, 1661
BmrFI CCNGG 9 cut(s) 1266, 1657, 1818, 2376, 2637, 3252, 3509, 3552, 3621
BoxI GACNNNNGTC 1 cut(s) 2235
BpiI GAAGAC 2 cut(s) 2412, 3299
BpmI CTGGAG 1 cut(s) 3612
Bpu1102I GCTNAGC 1 cut(s) 679
BpuEI CTTGAG 8 cut(s) 185, 377, 464, 1019, 2075, 2540, 2567, 2645
BpuMI CCSGG 2 cut(s) 1266, 1657
BsaBI GATNNNNATC 3 cut(s) 1764, 1851, 2208
BsaHI GRCGYC 1 cut(s) 891
BsaJI CCNNGG 4 cut(s) 1475, 2572, 3250, 3508
BsaWI WCCGGW 1 cut(s) 382
BsaXI ACNNNNNCTCC 8 cut(s) 202, 232, 844, 874, 908, 938, 3673, 3703
Bsc4I CCNNNNNNNGG 6 cut(s) 204, 446, 1082, 2067, 3116, 3508
Bse1I ACTGG 7 cut(s) 524, 1160, 1811, 2061, 2922, 3213, 3378
Bse3DI GCAATG 1 cut(s) 1286
Bse8I GATNNNNATC 3 cut(s) 1764, 1851, 2208
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 7 cut(s) 1818, 2376, 2637, 3252, 3509, 3552, 3621
BseDI CCNNGG 4 cut(s) 1475, 2572, 3250, 3508
BseGI GGATG 9 cut(s) 94, 475, 991, 1111, 1518, 1696, 2546, 2900, 3012
BseJI GATNNNNATC 3 cut(s) 1764, 1851, 2208
BseLI CCNNNNNNNGG 6 cut(s) 204, 446, 1082, 2067, 3116, 3508
BseMI GCAATG 1 cut(s) 1286
BseMII CTCAG 5 cut(s) 311, 2697, 2806, 3086, 3235
BseNI ACTGG 7 cut(s) 524, 1160, 1811, 2061, 2922, 3213, 3378
BseRI GAGGAG 1 cut(s) 829
BseX3I CGGCCG 1 cut(s) 845
BseYI CCCAGC 1 cut(s) 3566
BsgI GTGCAG 3 cut(s) 133, 1150, 1750
Bsh1285I CGRYCG 1 cut(s) 848
BshFI GGCC 7 cut(s) 847, 1307, 1626, 1797, 2799, 2910, 3324
BsiEI CGRYCG 1 cut(s) 848
BsiHKAI GWGCWC 1 cut(s) 2852
BsiHKCI CYCGRG 2 cut(s) 283, 1542
BsiSI CCGG 4 cut(s) 383, 693, 1265, 1656
BslFI GGGAC 3 cut(s) 317, 1158, 2347
BslI CCNNNNNNNGG 6 cut(s) 204, 446, 1082, 2067, 3116, 3508
BsmAI GTCTC 3 cut(s) 2516, 2661, 2874
BsmFI GGGAC 3 cut(s) 317, 1158, 2347
BsmI GAATGC 2 cut(s) 62, 3392
BsnI GGCC 7 cut(s) 847, 1307, 1626, 1797, 2799, 2910, 3324
BsoBI CYCGRG 2 cut(s) 283, 1542
Bsp1286I GDGCHC 1 cut(s) 2852
Bsp13I TCCGGA 1 cut(s) 382
Bsp1720I GCTNAGC 1 cut(s) 679
Bsp19I CCATGG 1 cut(s) 1475
BspACI CCGC 8 cut(s) 205, 280, 922, 961, 1441, 1516, 1958, 3103
BspANI GGCC 7 cut(s) 847, 1307, 1626, 1797, 2799, 2910, 3324
BspCNI CTCAG 5 cut(s) 312, 2698, 2805, 3087, 3234
BspEI TCCGGA 1 cut(s) 382
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 3 cut(s) 1263, 1437, 1661
BspMAI CTGCAG 1 cut(s) 1179
BspMI ACCTGC 2 cut(s) 546, 1225
BspPI GGATC 8 cut(s) 374, 1430, 1443, 1773, 1898, 2695, 2820, 2949
BsrBI CCGCTC 3 cut(s) 282, 924, 961
BsrDI GCAATG 1 cut(s) 1286
BsrI ACTGG 7 cut(s) 524, 1160, 1811, 2061, 2922, 3213, 3378
BssECI CCNNGG 4 cut(s) 1475, 2572, 3250, 3508
BssNAI GTATAC 1 cut(s) 1398
BssNI GRCGYC 1 cut(s) 891
BssSI CACGAG 2 cut(s) 153, 2556
BssT1I CCWWGG 2 cut(s) 1475, 2572
Bst1107I GTATAC 1 cut(s) 1398
Bst2BI CACGAG 2 cut(s) 153, 2556
Bst2UI CCWGG 7 cut(s) 1818, 2376, 2637, 3252, 3509, 3552, 3621
Bst4CI ACNGT 4 cut(s) 26, 1773, 1897, 2655
Bst6I CTCTTC 1 cut(s) 1251
BstACI GRCGYC 1 cut(s) 891
BstAPI GCANNNNNTGC 2 cut(s) 764, 2323
BstC8I GCNNGC 7 cut(s) 543, 1414, 1795, 2315, 2633, 3101, 3696
BstDSI CCRYGG 1 cut(s) 1475
BstENI CCTNNNNNAGG 1 cut(s) 3506
BstF5I GGATG 9 cut(s) 94, 475, 991, 1111, 1518, 1696, 2546, 2900, 3012
BstH2I RGCGCY 1 cut(s) 1290
BstHHI GCGC 1 cut(s) 1289
BstMAI GTCTC 3 cut(s) 2516, 2661, 2874
BstMCI CGRYCG 1 cut(s) 848
BstMWI GCNNNNNNNGC 5 cut(s) 764, 1864, 2323, 3471, 3576
BstNI CCWGG 7 cut(s) 1818, 2376, 2637, 3252, 3509, 3552, 3621
BstNSI RCATGY 7 cut(s) 767, 771, 1391, 1404, 1729, 2317, 3103
BstPAI GACNNNNGTC 1 cut(s) 2235
BstSCI CCNGG 9 cut(s) 1264, 1655, 1816, 2374, 2635, 3250, 3507, 3550, 3619
BstSFI CTRYAG 2 cut(s) 1175, 3069
BstV2I GAAGAC 2 cut(s) 2412, 3299
BstX2I RGATCY 9 cut(s) 316, 1138, 1435, 1903, 2530, 2700, 2812, 2941, 3136
BstYI RGATCY 9 cut(s) 316, 1138, 1435, 1903, 2530, 2700, 2812, 2941, 3136
BstZ17I GTATAC 1 cut(s) 1398
BstZI CGGCCG 1 cut(s) 845
BsuRI GGCC 7 cut(s) 847, 1307, 1626, 1797, 2799, 2910, 3324
BtgI CCRYGG 1 cut(s) 1475
BtgZI GCGATG 1 cut(s) 660
BtsCI GGATG 9 cut(s) 94, 475, 991, 1111, 1518, 1696, 2546, 2900, 3012
BtsI GCAGTG 1 cut(s) 663
BveI ACCTGC 2 cut(s) 546, 1225
Cac8I GCNNGC 7 cut(s) 543, 1414, 1795, 2315, 2633, 3101, 3696
CciI TCATGA 1 cut(s) 187
CfoI GCGC 1 cut(s) 1289
Cfr13I GGNCC 2 cut(s) 1814, 2988
CseI GACGC 1 cut(s) 2746
Csp6I GTAC 8 cut(s) 174, 238, 1086, 1190, 2597, 3339, 3545, 3702
CspCI CAANNNNNGTGG 2 cut(s) 3641, 3676
CviQI GTAC 8 cut(s) 174, 238, 1086, 1190, 2597, 3339, 3545, 3702
DraI TTTAAA 5 cut(s) 340, 396, 1032, 1935, 2262
DraIII CACNNNGTG 1 cut(s) 2419
EaeI YGGCCR 4 cut(s) 845, 1305, 1795, 2908
EagI CGGCCG 1 cut(s) 845
Eam1104I CTCTTC 1 cut(s) 1251
EarI CTCTTC 1 cut(s) 1251
EclXI CGGCCG 1 cut(s) 845
Eco130I CCWWGG 2 cut(s) 1475, 2572
Eco147I AGGCCT 1 cut(s) 3324
Eco47I GGWCC 2 cut(s) 1814, 2988
Eco47III AGCGCT 1 cut(s) 1288
Eco52I CGGCCG 1 cut(s) 845
Eco57I CTGAAG 4 cut(s) 1109, 2111, 2444, 3512
Eco88I CYCGRG 2 cut(s) 283, 1542
EcoNI CCTNNNNNAGG 1 cut(s) 3506
EcoRII CCWGG 7 cut(s) 1816, 2374, 2635, 3250, 3507, 3550, 3619
EcoT14I CCWWGG 2 cut(s) 1475, 2572
EcoT22I ATGCAT 5 cut(s) 769, 1727, 2319, 2546, 3394
ErhI CCWWGG 2 cut(s) 1475, 2572
FalI AAGNNNNNCTT 8 cut(s) 408, 440, 551, 583, 3272, 3304, 3531, 3563
FaqI GGGAC 3 cut(s) 317, 1158, 2347
FauI CCCGC 5 cut(s) 287, 929, 1448, 1509, 1965
FauNDI CATATG 1 cut(s) 1469
FbaI TGATCA 1 cut(s) 1555
FblI GTMKAC 4 cut(s) 915, 1397, 2735, 3664
FokI GGATG 9 cut(s) 101, 482, 998, 1118, 1525, 1703, 2553, 2907, 3019
GlaI GCGC 1 cut(s) 1288
GsaI CCCAGC 1 cut(s) 3570
GsuI CTGGAG 1 cut(s) 3612
HaeII RGCGCY 1 cut(s) 1290
HaeIII GGCC 7 cut(s) 847, 1307, 1626, 1797, 2799, 2910, 3324
HapII CCGG 4 cut(s) 383, 693, 1265, 1656
HgaI GACGC 1 cut(s) 2746
HhaI GCGC 1 cut(s) 1289
Hin1I GRCGYC 1 cut(s) 891
Hin6I GCGC 1 cut(s) 1287
HinP1I GCGC 1 cut(s) 1287
HincII GTYRAC 3 cut(s) 210, 852, 2736
HindII GTYRAC 3 cut(s) 210, 852, 2736
HindIII AAGCTT 3 cut(s) 543, 676, 3242
HpaII CCGG 4 cut(s) 383, 693, 1265, 1656
HphI GGTGA 4 cut(s) 429, 1065, 2237, 3176
Hpy166II GTNNAC 7 cut(s) 32, 210, 852, 916, 1398, 2736, 3665
Hpy8I GTNNAC 7 cut(s) 32, 210, 852, 916, 1398, 2736, 3665
Hpy99I CGWCG 4 cut(s) 11, 893, 1381, 2740
HpyCH4III ACNGT 4 cut(s) 26, 1773, 1897, 2655
HpyCH4IV ACGT 4 cut(s) 891, 1094, 2383, 2418
HpyF10VI GCNNNNNNNGC 5 cut(s) 764, 1864, 2323, 3471, 3576
HpySE526I ACGT 4 cut(s) 891, 1094, 2383, 2418
Hsp92I GRCGYC 1 cut(s) 891
HspAI GCGC 1 cut(s) 1287
Kpn2I TCCGGA 1 cut(s) 382
Ksp22I TGATCA 1 cut(s) 1555
LmnI GCTCC 7 cut(s) 642, 929, 1284, 1703, 1975, 3654, 3681
MaeII ACGT 4 cut(s) 891, 1094, 2383, 2418
MaeIII GTNAC 8 cut(s) 821, 1773, 2215, 2397, 2553, 2926, 3149, 3164
MbiI CCGCTC 3 cut(s) 282, 924, 961
MfeI CAATTG 3 cut(s) 121, 1038, 2781
MflI RGATCY 9 cut(s) 316, 1138, 1435, 1903, 2530, 2700, 2812, 2941, 3136
MhlI GDGCHC 1 cut(s) 2852
MlsI TGGCCA 3 cut(s) 1307, 1797, 2910
MluNI TGGCCA 3 cut(s) 1307, 1797, 2910
MlyI GAGTC 6 cut(s) 203, 404, 830, 1040, 2369, 3313
Mox20I TGGCCA 3 cut(s) 1307, 1797, 2910
Mph1103I ATGCAT 5 cut(s) 769, 1727, 2319, 2546, 3394
MroI TCCGGA 1 cut(s) 382
MroXI GAANNNNTTC 3 cut(s) 1917, 2349, 3497
MscI TGGCCA 3 cut(s) 1307, 1797, 2910
MslI CAYNNNNRTG 5 cut(s) 303, 2120, 2793, 2922, 2958
Msp20I TGGCCA 3 cut(s) 1307, 1797, 2910
MspA1I CMGCKG 1 cut(s) 3570
MspI CCGG 4 cut(s) 383, 693, 1265, 1656
MspR9I CCNGG 9 cut(s) 1266, 1657, 1818, 2376, 2637, 3252, 3509, 3552, 3621
MunI CAATTG 3 cut(s) 121, 1038, 2781
Mva1269I GAATGC 2 cut(s) 62, 3392
MvaI CCWGG 7 cut(s) 1818, 2376, 2637, 3252, 3509, 3552, 3621
MwoI GCNNNNNNNGC 5 cut(s) 764, 1864, 2323, 3471, 3576
NciI CCSGG 2 cut(s) 1266, 1657
NcoI CCATGG 1 cut(s) 1475
NdeI CATATG 1 cut(s) 1469
NlaIV GGNNCC 3 cut(s) 1263, 1437, 1661
NmeAIII GCCGAG 2 cut(s) 181, 823
NmuCI GTSAC 5 cut(s) 1773, 2215, 2397, 2553, 3164
NsiI ATGCAT 5 cut(s) 769, 1727, 2319, 2546, 3394
NspI RCATGY 7 cut(s) 767, 771, 1391, 1404, 1729, 2317, 3103
OliI CACNNNNGTG 1 cut(s) 2120
PaeI GCATGC 2 cut(s) 2317, 3103
PaeR7I CTCGAG 1 cut(s) 283
PagI TCATGA 1 cut(s) 187
PaqCI CACCTGC 1 cut(s) 546
PceI AGGCCT 1 cut(s) 3324
PciI ACATGT 1 cut(s) 1387
PctI GAATGC 2 cut(s) 62, 3392
PdmI GAANNNNTTC 3 cut(s) 1917, 2349, 3497
PfeI GAWTC 6 cut(s) 484, 1120, 2209, 2602, 2648, 2884
PflMI CCANNNNNTGG 2 cut(s) 1082, 2067
PfoI TCCNGGA 1 cut(s) 3550
PleI GAGTC 6 cut(s) 202, 404, 830, 1040, 2368, 3312
PpsI GAGTC 6 cut(s) 202, 404, 830, 1040, 2368, 3312
PscI ACATGT 1 cut(s) 1387
PshAI GACNNNNGTC 1 cut(s) 2235
PshBI ATTAAT 1 cut(s) 2256
PsiI TTATAA 2 cut(s) 257, 2193
Psp6I CCWGG 7 cut(s) 1816, 2374, 2635, 3250, 3507, 3550, 3619
PspFI CCCAGC 1 cut(s) 3566
PspGI CCWGG 7 cut(s) 1816, 2374, 2635, 3250, 3507, 3550, 3619
PspN4I GGNNCC 3 cut(s) 1263, 1437, 1661
PspPI GGNCC 2 cut(s) 1814, 2988
PspXI VCTCGAGB 1 cut(s) 283
PstI CTGCAG 1 cut(s) 1179
PsuI RGATCY 9 cut(s) 316, 1138, 1435, 1903, 2530, 2700, 2812, 2941, 3136
PvuII CAGCTG 1 cut(s) 3570
RsaI GTAC 8 cut(s) 175, 239, 1087, 1191, 2598, 3340, 3546, 3703
RsaNI GTAC 8 cut(s) 174, 238, 1086, 1190, 2597, 3339, 3545, 3702
RseI CAYNNNNRTG 5 cut(s) 303, 2120, 2793, 2922, 2958
SalI GTCGAC 1 cut(s) 2734
Sau96I GGNCC 2 cut(s) 1814, 2988
ScaI AGTACT 2 cut(s) 175, 3703
SchI GAGTC 6 cut(s) 203, 404, 830, 1040, 2369, 3313
ScrFI CCNGG 9 cut(s) 1266, 1657, 1818, 2376, 2637, 3252, 3509, 3552, 3621
SduI GDGCHC 1 cut(s) 2852
SfcI CTRYAG 2 cut(s) 1175, 3069
Sfr274I CTCGAG 1 cut(s) 283
SinI GGWCC 2 cut(s) 1814, 2988
SlaI CTCGAG 1 cut(s) 283
SmiMI CAYNNNNRTG 5 cut(s) 303, 2120, 2793, 2922, 2958
SmlI CTYRAG 9 cut(s) 164, 283, 356, 479, 998, 2090, 2519, 2582, 2624
SmoI CTYRAG 9 cut(s) 164, 283, 356, 479, 998, 2090, 2519, 2582, 2624
SpeI ACTAGT 3 cut(s) 176, 388, 3032
SphI GCATGC 2 cut(s) 2317, 3103
SseBI AGGCCT 1 cut(s) 3324
SsiI CCGC 8 cut(s) 205, 280, 922, 961, 1441, 1516, 1958, 3103
SspI AATATT 3 cut(s) 596, 1684, 2978
StuI AGGCCT 1 cut(s) 3324
StyD4I CCNGG 9 cut(s) 1264, 1655, 1816, 2374, 2635, 3250, 3507, 3550, 3619
StyI CCWWGG 2 cut(s) 1475, 2572
TaaI ACNGT 4 cut(s) 26, 1773, 1897, 2655
TaiI ACGT 4 cut(s) 894, 1097, 2386, 2421
TaqI TCGA 4 cut(s) 284, 2646, 2735, 2748
TaqII GACCGA 1 cut(s) 1802
TatI WGTACW 5 cut(s) 173, 2596, 3338, 3544, 3701
TfiI GAWTC 6 cut(s) 484, 1120, 2209, 2602, 2648, 2884
TseFI GTSAC 5 cut(s) 1773, 2215, 2397, 2553, 3164
Tsp45I GTSAC 5 cut(s) 1773, 2215, 2397, 2553, 3164
TspGWI ACGGA 2 cut(s) 1716, 2157
Van91I CCANNNNNTGG 2 cut(s) 1082, 2067
VpaK11BI GGWCC 2 cut(s) 1814, 2988
VspI ATTAAT 1 cut(s) 2256
XagI CCTNNNNNAGG 1 cut(s) 3506
XapI RAATTY 6 cut(s) 624, 775, 969, 1560, 3427, 3561
XceI RCATGY 7 cut(s) 767, 771, 1391, 1404, 1729, 2317, 3103
XcmI CCANNNNNNNNNTGG 1 cut(s) 3587
XhoI CTCGAG 1 cut(s) 283
XmiI GTMKAC 4 cut(s) 915, 1397, 2735, 3664
XmnI GAANNNNTTC 3 cut(s) 1917, 2349, 3497
ZraI GACGTC 1 cut(s) 892
ZrmI AGTACT 2 cut(s) 175, 3703
Zsp2I ATGCAT 5 cut(s) 769, 1727, 2319, 2546, 3394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.