RLG00000030354

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000105
Physical Location & Seq
Forward (+)
59962352 .. 59968838
6487 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030354

Sequence Viewer

Length: 2835 bp
ATGGGAACAACCCATGAGAACACCCCATCCAATATGGAAAACTCTAATCCAATGTGCCGCCGCCGATGTCCCTTAAAACAGATAGCCATGGATCATAATTGCTTCTTCTTCACCATGAAGAAACCATCAACCACCCACCCCGGATGTAATCACCATGCACCGATGAGGCCAGAGGCCCGTGTCGGCACTGGGGCTACTGGGGCTCCACCAGAAGGGAGTACCAAAGAGAAATTGGAAAAAACCAAGCCCTGTGCAAAACTACACTTTGCCGAGAAAGACGGAGCAATAGAAAAAGTTAGAGATGTCGTTACCCAATTCGAAGCCCGACTCCGAATCATCTTCCTCCTCAACCTCACAAAGCTTCACCGAGTGAAAATTTGTAAGGAGCACGAGCACTCCGACCTCGACAAGCACCTCTCCAATCTCCAGAAATCCTCCCAGGTCCTCGACATCGTCAAGTCCGACGCCGACCATGTCCTCTCCAACATCTCCTCCACCTGCGACCTTGCCTACCACGTCAGCGCCAATGTCCACACGCTCGACCTCGCCCAGTCGTGCGTCAACTCCATGCTCCTTCGTTTGGATGCGATTGTGGAGCTCGACCTCTCCCAACATCAAACAGAACTAATTGAGGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTGATAGGAATGGAGTCCCGCATTGATGATTTATTAAGCAATTACATATTTGCGCAGCTTGGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACAGTTGCTCGAGCTATCTATGATGAAATTTGTCAGGGTTTTTACCGAAGCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCCTTGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGGCAAAAGTTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGTCACAATTAGAGAAATTGGCTGGAAGTCGCAACTGGTTTGGTCCAGGGAGTAGAATTATCATAACCACCACAGATATTCAGTTGTTAAAGGCACATGATGTTGATGCTACGTACAAGGCTTATGGGTTAAACTGTGATGAAACACTGCAACTTTTGAGTTTGAAGGCTTTTAAGAAGTGTTCCCCACCAGAAGATTATTTGCAACTGTGCTACCATATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTTGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCACTGATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCAGATAAACGCATTATTGAGGTGCTTCGGATTAGTTTTGATGGCCTGGATGAAAAAGACAAAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGAGTGACACAAATACTAGACTATTGCAAGCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATAGATGTCTCATCACTATCTCCAACAACGAACTGTCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAATTGTTCGTGAACAGTCTCCTGAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACACTGTGCTGAAGAGAAATAAGAATTTTGACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGACCCCAGATCTCGGAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTGAGAATTTGGTAAGAATCCATAGATCCCTTGGATTTCACAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCAGGAGTCTGCCAAGTCAAATAAAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTTGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACGGCTGAGTGGCCTTGTGTCATTGAATCTAAGCAACTGCAAAAATCTTGTTTGTCTTCCAAGCACCATCAATAAATTGAAGTCTGTTGTTAATCTTTCTGGATGCTTGAAACTCCGCAAACATCAGGTAAATGTGGGGGAGATGGACTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGTCATCTACCCATGATCGCATAAAATATGTGAGAGGTTCAATCTTTCATGGATGCAAAGTAGTTTGGCGATCTTTAAATAATTTCTTGGCTTCTGGATTGGTGCAAAAAGTGAACACAGAGCCAATGAATTTCCGCTTGCCTATATCTGGTCTGTGTAATTTAACATATCTAAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCTGCAAATTTGGTTACTTTCCCTCATTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGCCTTCCTTCAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGATCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATAGCAATCAAGGATGCAGTAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGTACTCCCACTACAAGCGTGTGTGTTTCTGCTCATATACTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

945

Amino Acids

106.27

Weight (kDa)

8.03

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COG4_N PF20663 139 - 202 1.4e-09 Conserved oligomeric Golgi complex subunit 4, N-terminal
NB-ARC PF00931 252 - 407 2.7e-16 NB-ARC domain
NACHT PF05729 257 - 353 4.2e-06 NACHT domain
WHD_ROQ1 PF23282 474 - 545 6.6e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 582 - 675 3.4e-06 Leucine-rich repeat region
LRR_13 PF23286 642 - 726 1.9e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 506
Acc16I TGCGCA 1 cut(s) 747
Acc36I ACCTGC 1 cut(s) 506
AccI GTMKAC 2 cut(s) 668, 2620
AciI CCGC 7 cut(s) 58, 61, 712, 787, 994, 2173, 2383
AclI AACGTT 1 cut(s) 1999
AclWI GGATC 6 cut(s) 99, 769, 782, 1044, 1488, 1833
AcsI RAATTY 7 cut(s) 375, 831, 1708, 1819, 2377, 2465, 2677
AcuI CTGAAG 4 cut(s) 1659, 1715, 2514, 2792
AcyI GRCGYC 1 cut(s) 465
AdeI CACNNNGTG 1 cut(s) 370
AfaI GTAC 4 cut(s) 220, 1157, 2647, 2793
AfiI CCNNNNNNNGG 5 cut(s) 212, 580, 1526, 1772, 2396
AjiI CACGTC 1 cut(s) 517
AjnI CCWGG 4 cut(s) 438, 1087, 1416, 1645
AjuI GAANNNNNNNTTGG 2 cut(s) 2552, 2584
AloI GAACNNNNNNTCC 2 cut(s) 11, 43
AluBI AGCT 9 cut(s) 361, 598, 751, 818, 855, 977, 1513, 1861, 2554
AluI AGCT 9 cut(s) 361, 598, 751, 818, 855, 977, 1513, 1861, 2554
Alw21I GWGCWC 3 cut(s) 390, 396, 600
Alw26I GTCTC 8 cut(s) 1559, 1638, 1674, 1754, 1787, 1898, 2018, 2578
AlwI GGATC 6 cut(s) 99, 769, 782, 1044, 1488, 1833
AlwNI CAGNNNCTG 1 cut(s) 977
Ama87I CYCGRG 2 cut(s) 813, 1976
AoxI GGCC 5 cut(s) 167, 174, 895, 1414, 2068
ApeKI GCWGC 3 cut(s) 748, 855, 977
ApoI RAATTY 7 cut(s) 375, 831, 1708, 1819, 2377, 2465, 2677
AseI ATTAAT 1 cut(s) 1940
Asp700I GAANNNNTTC 3 cut(s) 918, 1620, 2777
AspLEI GCGC 2 cut(s) 524, 748
AspS9I GGNCC 3 cut(s) 175, 442, 1085
AsuC2I CCSGG 1 cut(s) 141
AsuHPI GGTGA 5 cut(s) 103, 143, 356, 2456, 2500
AsuII TTCGAA 1 cut(s) 318
AvaI CYCGRG 2 cut(s) 813, 1976
AvaII GGWCC 2 cut(s) 442, 1085
BamHI GGATCC 1 cut(s) 774
BanII GRGCYC 2 cut(s) 205, 600
BauI CACGAG 1 cut(s) 389
BbsI GAAGAC 3 cut(s) 1683, 2105, 2780
Bbv12I GWGCWC 3 cut(s) 390, 396, 600
BbvI GCAGC 3 cut(s) 760, 842, 964
BccI CCATC 8 cut(s) 34, 133, 931, 1406, 2132, 2194, 2617, 2633
BceAI ACGGC 1 cut(s) 2075
BciT130I CCWGG 4 cut(s) 440, 1089, 1418, 1647
BcnI CCSGG 1 cut(s) 141
BcoDI GTCTC 8 cut(s) 1559, 1638, 1674, 1754, 1787, 1898, 2018, 2578
BfaI CTAG 2 cut(s) 1499, 2624
BfoI RGCGCY 1 cut(s) 525
BfuAI ACCTGC 1 cut(s) 506
BglII AGATCT 3 cut(s) 1768, 1801, 2602
BisI GCNGC 5 cut(s) 58, 61, 749, 856, 978
BlsI GCNGC 5 cut(s) 59, 62, 750, 857, 979
Bme1390I CCNGG 5 cut(s) 141, 440, 1089, 1418, 1647
Bme18I GGWCC 2 cut(s) 442, 1085
BmeT110I CYCGRG 2 cut(s) 813, 1976
BmgBI CACGTC 1 cut(s) 517
BmgT120I GGNCC 3 cut(s) 175, 442, 1085
BmiI GGNNCC 2 cut(s) 204, 776
BmrFI CCNGG 5 cut(s) 141, 440, 1089, 1418, 1647
BmrI ACTGGG 3 cut(s) 198, 207, 544
BmsI GCATC 8 cut(s) 574, 1138, 1575, 2150, 2291, 2659, 2681, 2735
BmuI ACTGGG 3 cut(s) 198, 207, 544
BpiI GAAGAC 3 cut(s) 1683, 2105, 2780
BpmI CTGGAG 1 cut(s) 410
Bpu10I CCTNAGC 1 cut(s) 1275
Bpu14I TTCGAA 1 cut(s) 318
BpuEI CTTGAG 2 cut(s) 1811, 2576
BpuMI CCSGG 1 cut(s) 141
BsaAI YACGTR 1 cut(s) 1155
BsaBI GATNNNNATC 2 cut(s) 1035, 1479
BsaHI GRCGYC 1 cut(s) 465
BsaI GGTCTC 2 cut(s) 1674, 1754
BsaJI CCNNGG 6 cut(s) 87, 139, 438, 778, 1088, 1843
BsaXI ACNNNNNCTCC 6 cut(s) 187, 217, 380, 410, 476, 506
Bsc4I CCNNNNNNNGG 5 cut(s) 212, 580, 1526, 1772, 2396
Bse1I ACTGG 4 cut(s) 193, 202, 550, 1082
Bse8I GATNNNNATC 2 cut(s) 1035, 1479
BseBI CCWGG 4 cut(s) 440, 1089, 1418, 1647
BseDI CCNNGG 6 cut(s) 87, 139, 438, 778, 1088, 1843
BseGI GGATG 9 cut(s) 26, 149, 589, 967, 1426, 1817, 2165, 2306, 2750
BseJI GATNNNNATC 2 cut(s) 1035, 1479
BseLI CCNNNNNNNGG 5 cut(s) 212, 580, 1526, 1772, 2396
BseMII CTCAG 4 cut(s) 1289, 1909, 2054, 2417
BseNI ACTGG 4 cut(s) 193, 202, 550, 1082
BseRI GAGGAG 2 cut(s) 335, 481
BseXI GCAGC 3 cut(s) 760, 842, 964
BseYI CCCAGC 1 cut(s) 1611
BshFI GGCC 5 cut(s) 169, 176, 897, 1416, 2070
BsiHKAI GWGCWC 3 cut(s) 390, 396, 600
BsiHKCI CYCGRG 2 cut(s) 813, 1976
BsiSI CCGG 1 cut(s) 141
BslFI GGGAC 2 cut(s) 54, 694
BslI CCNNNNNNNGG 5 cut(s) 212, 580, 1526, 1772, 2396
BsmAI GTCTC 8 cut(s) 1559, 1638, 1674, 1754, 1787, 1898, 2018, 2578
BsmFI GGGAC 2 cut(s) 54, 694
BsnI GGCC 5 cut(s) 169, 176, 897, 1416, 2070
Bso31I GGTCTC 2 cut(s) 1674, 1754
BsoBI CYCGRG 2 cut(s) 813, 1976
Bsp119I TTCGAA 1 cut(s) 318
Bsp1286I GDGCHC 4 cut(s) 205, 390, 396, 600
Bsp19I CCATGG 2 cut(s) 87, 778
BspACI CCGC 7 cut(s) 58, 61, 712, 787, 994, 2173, 2383
BspANI GGCC 5 cut(s) 169, 176, 897, 1416, 2070
BspCNI CTCAG 4 cut(s) 1288, 1908, 2055, 2418
BspHI TCATGA 1 cut(s) 1672
BspLI GGNNCC 2 cut(s) 204, 776
BspMI ACCTGC 1 cut(s) 506
BspPI GGATC 6 cut(s) 99, 769, 782, 1044, 1488, 1833
BspQI GCTCTTC 1 cut(s) 1635
BspT104I TTCGAA 1 cut(s) 318
BspTNI GGTCTC 2 cut(s) 1674, 1754
BsrI ACTGG 4 cut(s) 193, 202, 550, 1082
BssECI CCNNGG 6 cut(s) 87, 139, 438, 778, 1088, 1843
BssNAI GTATAC 1 cut(s) 669
BssNI GRCGYC 1 cut(s) 465
BssSI CACGAG 1 cut(s) 389
BssT1I CCWWGG 3 cut(s) 87, 778, 1843
Bst1107I GTATAC 1 cut(s) 669
Bst2BI CACGAG 1 cut(s) 389
Bst2UI CCWGG 4 cut(s) 440, 1089, 1418, 1647
Bst4CI ACNGT 7 cut(s) 808, 1178, 1251, 1581, 1632, 1690, 2207
Bst6I CTCTTC 2 cut(s) 1635, 1691
BstACI GRCGYC 1 cut(s) 465
BstAPI GCANNNNNTGC 1 cut(s) 1594
BstBAI YACGTR 1 cut(s) 1155
BstBI TTCGAA 1 cut(s) 318
BstC8I GCNNGC 5 cut(s) 785, 1456, 1511, 2387, 2461
BstDEI CTNAG 7 cut(s) 1275, 1895, 2063, 2087, 2426, 2501, 2550
BstDSI CCRYGG 2 cut(s) 87, 778
BstEII GGTNACC 1 cut(s) 2488
BstF5I GGATG 9 cut(s) 26, 149, 589, 967, 1426, 1817, 2165, 2306, 2750
BstH2I RGCGCY 1 cut(s) 525
BstHHI GCGC 2 cut(s) 524, 748
BstMAI GTCTC 8 cut(s) 1559, 1638, 1674, 1754, 1787, 1898, 2018, 2578
BstMWI GCNNNNNNNGC 4 cut(s) 200, 789, 1594, 2551
BstNI CCWGG 4 cut(s) 440, 1089, 1418, 1647
BstNSI RCATGY 1 cut(s) 787
BstPI GGTNACC 1 cut(s) 2488
BstSCI CCNGG 5 cut(s) 139, 438, 1087, 1416, 1645
BstSNI TACGTA 1 cut(s) 1155
BstV1I GCAGC 3 cut(s) 760, 842, 964
BstV2I GAAGAC 3 cut(s) 1683, 2105, 2780
BstX2I RGATCY 5 cut(s) 774, 1768, 1801, 1838, 2602
BstYI RGATCY 5 cut(s) 774, 1768, 1801, 1838, 2602
BstZ17I GTATAC 1 cut(s) 669
BsuRI GGCC 5 cut(s) 169, 176, 897, 1416, 2070
BtgI CCRYGG 2 cut(s) 87, 778
BtrI CACGTC 1 cut(s) 517
BtsCI GGATG 9 cut(s) 26, 149, 589, 967, 1426, 1817, 2165, 2306, 2750
BtsI GCAGTG 1 cut(s) 1187
BtsIMutI CAGTG 5 cut(s) 186, 1187, 1329, 1686, 2651
BveI ACCTGC 1 cut(s) 506
Cac8I GCNNGC 5 cut(s) 785, 1456, 1511, 2387, 2461
CaiI CAGNNNCTG 1 cut(s) 977
CciI TCATGA 1 cut(s) 1672
CfoI GCGC 2 cut(s) 524, 748
Cfr13I GGNCC 3 cut(s) 175, 442, 1085
CseI GACGC 2 cut(s) 473, 547
Csp6I GTAC 4 cut(s) 219, 1156, 2646, 2792
CviQI GTAC 4 cut(s) 219, 1156, 2646, 2792
DdeI CTNAG 7 cut(s) 1275, 1895, 2063, 2087, 2426, 2501, 2550
DraI TTTAAA 1 cut(s) 2325
DraIII CACNNNGTG 1 cut(s) 370
Eam1104I CTCTTC 2 cut(s) 1635, 1691
EarI CTCTTC 2 cut(s) 1635, 1691
Ecl136II GAGCTC 1 cut(s) 598
Eco105I TACGTA 1 cut(s) 1155
Eco130I CCWWGG 3 cut(s) 87, 778, 1843
Eco24I GRGCYC 2 cut(s) 205, 600
Eco31I GGTCTC 2 cut(s) 1674, 1754
Eco47I GGWCC 2 cut(s) 442, 1085
Eco53kI GAGCTC 1 cut(s) 598
Eco57I CTGAAG 4 cut(s) 1659, 1715, 2514, 2792
Eco88I CYCGRG 2 cut(s) 813, 1976
Eco91I GGTNACC 1 cut(s) 2488
EcoICRI GAGCTC 1 cut(s) 598
EcoO109I RGGNCCY 1 cut(s) 442
EcoO65I GGTNACC 1 cut(s) 2488
EcoRII CCWGG 4 cut(s) 438, 1087, 1416, 1645
EcoT14I CCWWGG 3 cut(s) 87, 778, 1843
EcoT22I ATGCAT 2 cut(s) 1590, 2674
EcoT38I GRGCYC 2 cut(s) 205, 600
ErhI CCWWGG 3 cut(s) 87, 778, 1843
FalI AAGNNNNNCTT 2 cut(s) 903, 935
FaqI GGGAC 2 cut(s) 54, 694
FauI CCCGC 2 cut(s) 719, 780
FblI GTMKAC 2 cut(s) 668, 2620
Fnu4HI GCNGC 5 cut(s) 58, 61, 749, 856, 978
FokI GGATG 9 cut(s) 13, 156, 596, 974, 1433, 1824, 2172, 2313, 2757
FriOI GRGCYC 2 cut(s) 205, 600
Fsp4HI GCNGC 5 cut(s) 58, 61, 749, 856, 978
FspBI CTAG 2 cut(s) 1499, 2624
FspI TGCGCA 1 cut(s) 747
GlaI GCGC 2 cut(s) 523, 747
GluI GCNGC 5 cut(s) 58, 61, 749, 856, 978
GsaI CCCAGC 1 cut(s) 1615
GsuI CTGGAG 1 cut(s) 410
HaeII RGCGCY 1 cut(s) 525
HaeIII GGCC 5 cut(s) 169, 176, 897, 1416, 2070
HapII CCGG 1 cut(s) 141
HgaI GACGC 2 cut(s) 473, 547
HhaI GCGC 2 cut(s) 524, 748
Hin1I GRCGYC 1 cut(s) 465
Hin6I GCGC 2 cut(s) 522, 746
HinP1I GCGC 2 cut(s) 522, 746
HincII GTYRAC 2 cut(s) 562, 685
HindII GTYRAC 2 cut(s) 562, 685
HindIII AAGCTT 3 cut(s) 359, 1859, 2552
HpaII CCGG 1 cut(s) 141
HphI GGTGA 5 cut(s) 103, 143, 356, 2456, 2500
Hpy166II GTNNAC 7 cut(s) 532, 562, 669, 685, 1628, 2362, 2621
Hpy188I TCNGA 7 cut(s) 332, 400, 463, 1401, 1775, 1785, 2602
Hpy8I GTNNAC 7 cut(s) 532, 562, 669, 685, 1628, 2362, 2621
Hpy99I CGWCG 1 cut(s) 467
HpyAV CCTTC 8 cut(s) 206, 584, 1201, 1465, 1802, 2441, 2534, 2538
HpyCH4III ACNGT 7 cut(s) 808, 1178, 1251, 1581, 1632, 1690, 2207
HpyCH4IV ACGT 4 cut(s) 516, 1154, 1654, 1999
HpyF10VI GCNNNNNNNGC 4 cut(s) 200, 789, 1594, 2551
HpyF3I CTNAG 7 cut(s) 1275, 1895, 2063, 2087, 2426, 2501, 2550
HpySE526I ACGT 4 cut(s) 516, 1154, 1654, 1999
Hsp92I GRCGYC 1 cut(s) 465
HspAI GCGC 2 cut(s) 522, 746
LguI GCTCTTC 1 cut(s) 1635
LmnI GCTCC 7 cut(s) 208, 281, 385, 576, 595, 974, 2450
Lsp1109I GCAGC 3 cut(s) 760, 842, 964
LweI GCATC 8 cut(s) 574, 1138, 1575, 2150, 2291, 2659, 2681, 2735
MaeI CTAG 2 cut(s) 1499, 2624
MaeII ACGT 4 cut(s) 516, 1154, 1654, 1999
MaeIII GTNAC 6 cut(s) 307, 1044, 1486, 2471, 2488, 2750
MfeI CAATTG 2 cut(s) 2052, 2716
MflI RGATCY 5 cut(s) 774, 1768, 1801, 1838, 2602
MhlI GDGCHC 4 cut(s) 205, 390, 396, 600
MlyI GAGTC 3 cut(s) 321, 716, 1909
MmeI TCCRAC 5 cut(s) 423, 486, 507, 1593, 1966
Mph1103I ATGCAT 2 cut(s) 1590, 2674
MroXI GAANNNNTTC 3 cut(s) 918, 1620, 2777
MspI CCGG 1 cut(s) 141
MspR9I CCNGG 5 cut(s) 141, 440, 1089, 1418, 1647
MunI CAATTG 2 cut(s) 2052, 2716
MvaI CCWGG 4 cut(s) 440, 1089, 1418, 1647
MwoI GCNNNNNNNGC 4 cut(s) 200, 789, 1594, 2551
NciI CCSGG 1 cut(s) 141
NcoI CCATGG 2 cut(s) 87, 778
NlaIV GGNNCC 2 cut(s) 204, 776
NmeAIII GCCGAG 1 cut(s) 295
NmuCI GTSAC 3 cut(s) 1044, 1486, 2488
NsbI TGCGCA 1 cut(s) 747
NsiI ATGCAT 2 cut(s) 1590, 2674
NspI RCATGY 1 cut(s) 787
NspV TTCGAA 1 cut(s) 318
PaeI GCATGC 1 cut(s) 787
PaeR7I CTCGAG 1 cut(s) 813
PagI TCATGA 1 cut(s) 1672
PaqCI CACCTGC 1 cut(s) 506
PciSI GCTCTTC 1 cut(s) 1635
PcsI WCGNNNNNNNCGW 2 cut(s) 396, 459
PdmI GAANNNNTTC 3 cut(s) 918, 1620, 2777
PfeI GAWTC 9 cut(s) 333, 930, 1786, 1830, 1873, 1925, 1972, 2083, 2497
PflFI GACNNNGTC 2 cut(s) 452, 473
PkrI GCNGC 5 cut(s) 59, 62, 750, 857, 979
PleI GAGTC 3 cut(s) 321, 715, 1908
PpsI GAGTC 3 cut(s) 321, 715, 1908
Ppu21I YACGTR 1 cut(s) 1155
PpuMI RGGWCCY 1 cut(s) 442
PshBI ATTAAT 1 cut(s) 1940
Psp124BI GAGCTC 1 cut(s) 600
Psp1406I AACGTT 1 cut(s) 1999
Psp5II RGGWCCY 1 cut(s) 442
Psp6I CCWGG 4 cut(s) 438, 1087, 1416, 1645
PspEI GGTNACC 1 cut(s) 2488
PspFI CCCAGC 1 cut(s) 1611
PspGI CCWGG 4 cut(s) 438, 1087, 1416, 1645
PspN4I GGNNCC 2 cut(s) 204, 776
PspPI GGNCC 3 cut(s) 175, 442, 1085
PspPPI RGGWCCY 1 cut(s) 442
PspXI VCTCGAGB 1 cut(s) 813
PstNI CAGNNNCTG 1 cut(s) 977
PsuI RGATCY 5 cut(s) 774, 1768, 1801, 1838, 2602
PsyI GACNNNGTC 2 cut(s) 452, 473
RsaI GTAC 4 cut(s) 220, 1157, 2647, 2793
RsaNI GTAC 4 cut(s) 219, 1156, 2646, 2792
SacI GAGCTC 1 cut(s) 600
SapI GCTCTTC 1 cut(s) 1635
SatI GCNGC 5 cut(s) 58, 61, 749, 856, 978
Sau96I GGNCC 3 cut(s) 175, 442, 1085
SchI GAGTC 3 cut(s) 321, 716, 1909
ScrFI CCNGG 5 cut(s) 141, 440, 1089, 1418, 1647
SduI GDGCHC 4 cut(s) 205, 390, 396, 600
SfaNI GCATC 8 cut(s) 574, 1138, 1575, 2150, 2291, 2659, 2681, 2735
Sfr274I CTCGAG 1 cut(s) 813
SfuI TTCGAA 1 cut(s) 318
SinI GGWCC 2 cut(s) 442, 1085
SlaI CTCGAG 1 cut(s) 813
SmlI CTYRAG 3 cut(s) 813, 1790, 2555
SmoI CTYRAG 3 cut(s) 813, 1790, 2555
SnaBI TACGTA 1 cut(s) 1155
SphI GCATGC 1 cut(s) 787
SsiI CCGC 7 cut(s) 58, 61, 712, 787, 994, 2173, 2383
SspI AATATT 1 cut(s) 1440
SspMI CTAG 2 cut(s) 1499, 2624
SstI GAGCTC 1 cut(s) 600
StyD4I CCNGG 5 cut(s) 139, 438, 1087, 1416, 1645
StyI CCWWGG 3 cut(s) 87, 778, 1843
TaaI ACNGT 7 cut(s) 808, 1178, 1251, 1581, 1632, 1690, 2207
TaiI ACGT 4 cut(s) 519, 1157, 1657, 2002
TaqI TCGA 8 cut(s) 318, 405, 447, 540, 600, 814, 1483, 1583
TatI WGTACW 1 cut(s) 2645
TauI GCSGC 2 cut(s) 60, 63
TfiI GAWTC 9 cut(s) 333, 930, 1786, 1830, 1873, 1925, 1972, 2083, 2497
TscAI CASTG 5 cut(s) 193, 1194, 1336, 1693, 2658
TseFI GTSAC 3 cut(s) 1044, 1486, 2488
TseI GCWGC 3 cut(s) 748, 855, 977
Tsp45I GTSAC 3 cut(s) 1044, 1486, 2488
TspGWI ACGGA 3 cut(s) 294, 652, 1275
TspRI CASTG 5 cut(s) 193, 1194, 1336, 1693, 2658
Tth111I GACNNNGTC 2 cut(s) 452, 473
VpaK11BI GGWCC 2 cut(s) 442, 1085
VspI ATTAAT 1 cut(s) 1940
XapI RAATTY 7 cut(s) 375, 831, 1708, 1819, 2377, 2465, 2677
XceI RCATGY 1 cut(s) 787
XcmI CCANNNNNNNNNTGG 3 cut(s) 229, 934, 1841
XhoI CTCGAG 1 cut(s) 813
XmiI GTMKAC 2 cut(s) 668, 2620
XmnI GAANNNNTTC 3 cut(s) 918, 1620, 2777
XspI CTAG 2 cut(s) 1499, 2624
Zsp2I ATGCAT 2 cut(s) 1590, 2674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.