RchiOBHm_Chr1g0323161

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000105
Physical Location & Seq
Reverse (-)
10688007 .. 10693549
5543 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55309

Sequence Viewer

Length: 2466 bp
ATGTTGATGCTACATACAAGGTTAACGGATTATTTGCATCTGTGCTACCGTATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTGTTGTGGTTTTAGGTTCTTTTCTGTTTGGTAGAAGCGCTAATGAATGGGCAAGTGCAATAGATAGGCTAAAGAACACACCACATAAACATATTATTGAGGTACTTCGGATTAGTTTTGATGGACTGGATCAAAAAGACAAAGAAATATTCCTACATATCGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTAGAATATTGCCAGCTAAACCCTGTCATTGGTTTAAGTGTTCTTGCTGATAGATCTCTCATAACTATCTCCGACGACAAAGAACTGTCTATGCATGATTTGCTACAAGAAACGGGCTGGGAAATTGTTCGTGAACAGTCTCCTACAGATCCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAAGAATGTGCTGAACAGAAATAAGGGAACAGATTCAATCCGAGGCATGGTAATGGAGTTAACTAAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTATTCTTCTCCATATTCGTAATGTGGACCTTCCCAATGGCCTCACTTGTCTTTCTAATTCCTTGAGATTCCTTGAATGGACTGGGTATCCCTTAATATCTCTCCCACAAAATTTCGAAGCAGATGAACTTGTTGAACTTAACCTGTGCCACAGCAACATTGAACAGCTTTGGAAGGGAACAAAGAATTTTGACGAGTTGAAGTTCATCAAACTTTGCCATTCTCGAAACATTGTGGAGACCCCAGACCTCACAGGCGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGGTGTGAGAATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAGTCTCAAGAGTCTGCCAAGTAGAGTTGAAACGGAATGTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTCGGTGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCAGTGTCAATTGAACGGCTGAGTGGCCTTGTGTCATTAAATCTGAGCAACTGCAGAAATCTTTTCTGTCTTCCGAGTACCATCAATAAATTGAAGTCTATTAAAGATCTTAATCTTTCTGGATGCTTGAAACTTGGCAAACATCAGGTAAATGTGGTGGAGATGGACTGTTTTGAGGAAACTCTTGTGAATAGTGGGACTGCAATAGAAATGTCATCTACCCATGATCGCAGAAAATATGTGAGAGGTTCAATCTTTGGCAAAGTGGTTTGGCGATCTTTAAATAAGTTCTTGCCTTCTGGGTTGGTGCAAAAAAGGAGTTTCCGCTTGCCTATATCTGGTCTGTGTAATTTAACATATCTGAACCTGAGTAATTGCAATCTTGGTAAAAGAGCATTTGCCAACGAATTTGGTTACTTTCCTTCTTTGGTGACCTTGAATCTAAGTGGGAACAATTTTGTTCGTCTTCCTTCAGGTATTGGATTGCTTCCTATGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTAAAGAGTTGTCGGACCTTCCATCAAATAGTATACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATCAAACAAATCATATTTTAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACATAGCATTCAAAATGCTGAAGGCATTCATGTATCAGGGAATCTCTAATAACAGGGAAACTTTTCAAATTGTAATTCCTGGAAGGAATATTCCTGAGTGGTTCAGCCATTGGAGTGTTGGGTGTTCTGTAAGTGTATCTCTACCTGCACATTCGAAAAACAGTCGGGTTTTGGGATTTGTTTTGTGTGCTGTTTTTGTAGTCCAAGAGCACCATCGGATTGATAAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCGATGGAAGAGAATTGGAAATGTATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCAAGGTTAAGTCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGGGTGGTGGCATAACAGTTACAGTCAGTTTGAGTTCTTATTTGAAACCAGAGGGCCAGGTCTGCAGGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAAGAAGTGCAAGAGTTGAACCAAACAACCACTCAATCAAGCAGTAGGATGTCTCCTTATGAGGATATATTGATTGATTTTGACATTCCGGTTGAAGGGGAAACCAGTGGCACTGGTAGCAGAACTTGCACGCTTGAAGAATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

821

Amino Acids

93.69

Weight (kDa)

7.05

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 72 - 143 5.7e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 241 - 259 3.3e-07 Leucine Rich Repeat
LRR_14 PF23598 303 - 413 2.7e-09 Leucine-rich repeat region
LRR_13 PF23286 333 - 418 7.1e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 628 - 777 2.2e-16 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2275
Acc36I ACCTGC 2 cut(s) 1960, 2275
AccI GTMKAC 1 cut(s) 1675
AciI CCGC 1 cut(s) 1438
AclI AACGTT 1 cut(s) 1069
AclWI GGATC 3 cut(s) 219, 279, 431
AcsI RAATTY 8 cut(s) 580, 703, 778, 889, 1520, 1732, 2048, 2153
AcuI CTGAAG 3 cut(s) 1569, 1847, 2111
AfaI GTAC 4 cut(s) 186, 1192, 1702, 2218
AfeI AGCGCT 1 cut(s) 121
AfiI CCNNNNNNNGG 4 cut(s) 317, 523, 1451, 2414
AjnI CCWGG 3 cut(s) 439, 1885, 2275
AjuI GAANNNNNNNTTGG 2 cut(s) 1607, 1639
AluBI AGCT 5 cut(s) 304, 760, 931, 2035, 2095
AluI AGCT 5 cut(s) 304, 760, 931, 2035, 2095
Alw21I GWGCWC 1 cut(s) 2019
Alw26I GTCTC 8 cut(s) 432, 468, 591, 824, 968, 1088, 1633, 2376
AlwI GGATC 3 cut(s) 219, 279, 431
Ama87I CYCGRG 1 cut(s) 1046
Aor51HI AGCGCT 1 cut(s) 121
AoxI GGCC 3 cut(s) 631, 1138, 2273
ApoI RAATTY 8 cut(s) 580, 703, 778, 889, 1520, 1732, 2048, 2153
AseI ATTAAT 1 cut(s) 1010
Asp700I GAANNNNTTC 4 cut(s) 414, 508, 1832, 1869
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 3 cut(s) 619, 1657, 2273
AsuHPI GGTGA 3 cut(s) 1555, 2168, 2299
AsuII TTCGAA 2 cut(s) 708, 1961
AvaI CYCGRG 1 cut(s) 1046
AvaII GGWCC 2 cut(s) 619, 1657
BauI CACGAG 1 cut(s) 2199
BbsI GAAGAC 3 cut(s) 477, 1175, 1571
Bbv12I GWGCWC 1 cut(s) 2019
BccI CCATC 8 cut(s) 197, 912, 1202, 1270, 1672, 1688, 2028, 2093
BceAI ACGGC 1 cut(s) 1145
BciT130I CCWGG 3 cut(s) 441, 1887, 2277
BciVI GTATCC 1 cut(s) 690
BcoDI GTCTC 8 cut(s) 432, 468, 591, 824, 968, 1088, 1633, 2376
BfaI CTAG 2 cut(s) 290, 1679
BfmI CTRYAG 3 cut(s) 432, 1165, 2282
BfoI RGCGCY 1 cut(s) 123
BfuAI ACCTGC 2 cut(s) 1960, 2275
BfuI GTATCC 1 cut(s) 690
BglII AGATCT 3 cut(s) 341, 871, 1219
Bme1390I CCNGG 3 cut(s) 441, 1887, 2277
Bme18I GGWCC 2 cut(s) 619, 1657
BmeT110I CYCGRG 1 cut(s) 1046
BmgT120I GGNCC 3 cut(s) 619, 1657, 2273
BmrFI CCNGG 3 cut(s) 441, 1887, 2277
BmrI ACTGGG 1 cut(s) 684
BmsI GCATC 5 cut(s) 46, 1226, 1714, 1736, 1790
BmuI ACTGGG 1 cut(s) 684
BpiI GAAGAC 3 cut(s) 477, 1175, 1571
Bpu10I CCTNAGC 1 cut(s) 66
Bpu14I TTCGAA 2 cut(s) 708, 1961
BpuEI CTTGAG 4 cut(s) 676, 881, 950, 1631
BsaBI GATNNNNATC 2 cut(s) 270, 1224
BsaI GGTCTC 2 cut(s) 468, 824
BsaJI CCNNGG 2 cut(s) 517, 913
BsaWI WCCGGW 1 cut(s) 2407
Bsc4I CCNNNNNNNGG 4 cut(s) 317, 523, 1451, 2414
Bse1I ACTGG 5 cut(s) 213, 679, 1115, 2424, 2437
Bse8I GATNNNNATC 2 cut(s) 270, 1224
BseBI CCWGG 3 cut(s) 441, 1887, 2277
BseDI CCNNGG 2 cut(s) 517, 913
BseGI GGATG 3 cut(s) 1241, 1805, 2373
BseJI GATNNNNATC 2 cut(s) 270, 1224
BseLI CCNNNNNNNGG 4 cut(s) 317, 523, 1451, 2414
BseMII CTCAG 5 cut(s) 80, 1124, 1148, 1472, 1893
BseNI ACTGG 5 cut(s) 213, 679, 1115, 2424, 2437
BseYI CCCAGC 1 cut(s) 405
BsgI GTGCAG 1 cut(s) 1938
BshFI GGCC 3 cut(s) 633, 1140, 2275
BsiHKAI GWGCWC 1 cut(s) 2019
BsiHKCI CYCGRG 1 cut(s) 1046
BsiSI CCGG 1 cut(s) 2408
BslFI GGGAC 1 cut(s) 1324
BslI CCNNNNNNNGG 4 cut(s) 317, 523, 1451, 2414
BsmAI GTCTC 8 cut(s) 432, 468, 591, 824, 968, 1088, 1633, 2376
BsmFI GGGAC 1 cut(s) 1324
BsmI GAATGC 2 cut(s) 1814, 1832
BsnI GGCC 3 cut(s) 633, 1140, 2275
Bso31I GGTCTC 2 cut(s) 468, 824
BsoBI CYCGRG 1 cut(s) 1046
Bsp119I TTCGAA 2 cut(s) 708, 1961
Bsp1286I GDGCHC 1 cut(s) 2019
BspACI CCGC 1 cut(s) 1438
BspANI GGCC 3 cut(s) 633, 1140, 2275
BspCNI CTCAG 5 cut(s) 79, 1125, 1149, 1473, 1894
BspHI TCATGA 1 cut(s) 466
BspMAI CTGCAG 2 cut(s) 1169, 2286
BspMI ACCTGC 2 cut(s) 1960, 2275
BspPI GGATC 3 cut(s) 219, 279, 431
BspT104I TTCGAA 2 cut(s) 708, 1961
BspTNI GGTCTC 2 cut(s) 468, 824
BsrI ACTGG 5 cut(s) 213, 679, 1115, 2424, 2437
BssECI CCNNGG 2 cut(s) 517, 913
BssNAI GTATAC 1 cut(s) 1676
BssSI CACGAG 1 cut(s) 2199
BssT1I CCWWGG 1 cut(s) 913
Bst1107I GTATAC 1 cut(s) 1676
Bst2BI CACGAG 1 cut(s) 2199
Bst2UI CCWGG 3 cut(s) 441, 1887, 2277
Bst4CI ACNGT 7 cut(s) 50, 375, 426, 1283, 1970, 2237, 2243
Bst6I CTCTTC 1 cut(s) 2098
BstAPI GCANNNNNTGC 3 cut(s) 388, 2326, 2445
BstBI TTCGAA 2 cut(s) 708, 1961
BstC8I GCNNGC 5 cut(s) 247, 302, 1442, 2133, 2450
BstDEI CTNAG 6 cut(s) 66, 1133, 1157, 1481, 1556, 1902
BstEII GGTNACC 1 cut(s) 1543
BstF5I GGATG 3 cut(s) 1241, 1805, 2373
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 1 cut(s) 122
BstMAI GTCTC 8 cut(s) 432, 468, 591, 824, 968, 1088, 1633, 2376
BstMWI GCNNNNNNNGC 6 cut(s) 388, 1606, 2281, 2326, 2436, 2445
BstNI CCWGG 3 cut(s) 441, 1887, 2277
BstPI GGTNACC 1 cut(s) 1543
BstSCI CCNGG 3 cut(s) 439, 1885, 2275
BstSFI CTRYAG 3 cut(s) 432, 1165, 2282
BstV2I GAAGAC 3 cut(s) 477, 1175, 1571
BstX2I RGATCY 4 cut(s) 341, 436, 871, 1219
BstYI RGATCY 4 cut(s) 341, 436, 871, 1219
BstZ17I GTATAC 1 cut(s) 1676
BsuI GTATCC 1 cut(s) 690
BsuRI GGCC 3 cut(s) 633, 1140, 2275
BtsCI GGATG 3 cut(s) 1241, 1805, 2373
BtsIMutI CAGTG 4 cut(s) 1122, 1706, 2430, 2431
BveI ACCTGC 2 cut(s) 1960, 2275
Cac8I GCNNGC 5 cut(s) 247, 302, 1442, 2133, 2450
CciI TCATGA 1 cut(s) 466
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 3 cut(s) 619, 1657, 2273
Csp6I GTAC 4 cut(s) 185, 1191, 1701, 2217
CviAII CATG 5 cut(s) 383, 467, 523, 1337, 1837
CviQI GTAC 4 cut(s) 185, 1191, 1701, 2217
DdeI CTNAG 6 cut(s) 66, 1133, 1157, 1481, 1556, 1902
DraI TTTAAA 1 cut(s) 1395
Eam1104I CTCTTC 1 cut(s) 2098
EarI CTCTTC 1 cut(s) 2098
Eco130I CCWWGG 1 cut(s) 913
Eco31I GGTCTC 2 cut(s) 468, 824
Eco47I GGWCC 2 cut(s) 619, 1657
Eco47III AGCGCT 1 cut(s) 121
Eco57I CTGAAG 3 cut(s) 1569, 1847, 2111
Eco88I CYCGRG 1 cut(s) 1046
Eco91I GGTNACC 1 cut(s) 1543
EcoO65I GGTNACC 1 cut(s) 1543
EcoRI GAATTC 1 cut(s) 2048
EcoRII CCWGG 3 cut(s) 439, 1885, 2275
EcoT14I CCWWGG 1 cut(s) 913
EcoT22I ATGCAT 2 cut(s) 384, 1729
ErhI CCWWGG 1 cut(s) 913
FaeI CATG 5 cut(s) 386, 470, 526, 1340, 1840
FaqI GGGAC 1 cut(s) 1324
FatI CATG 5 cut(s) 382, 466, 522, 1336, 1836
FblI GTMKAC 1 cut(s) 1675
FokI GGATG 3 cut(s) 1248, 1812, 2380
FspBI CTAG 2 cut(s) 290, 1679
GlaI GCGC 1 cut(s) 121
GsaI CCCAGC 1 cut(s) 409
HaeII RGCGCY 1 cut(s) 123
HaeIII GGCC 3 cut(s) 633, 1140, 2275
HapII CCGG 1 cut(s) 2408
HhaI GCGC 1 cut(s) 122
Hin1II CATG 5 cut(s) 386, 470, 526, 1340, 1840
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HincII GTYRAC 2 cut(s) 24, 537
HindII GTYRAC 2 cut(s) 24, 537
HindIII AAGCTT 2 cut(s) 929, 2033
HpaI GTTAAC 2 cut(s) 24, 537
HpaII CCGG 1 cut(s) 2408
HphI GGTGA 3 cut(s) 1555, 2168, 2299
Hpy166II GTNNAC 5 cut(s) 24, 422, 537, 619, 1676
Hpy8I GTNNAC 5 cut(s) 24, 422, 537, 619, 1676
Hpy99I CGWCG 1 cut(s) 365
HpyCH4III ACNGT 7 cut(s) 50, 375, 426, 1283, 1970, 2237, 2243
HpyCH4IV ACGT 2 cut(s) 448, 1069
HpyF10VI GCNNNNNNNGC 6 cut(s) 388, 1606, 2281, 2326, 2436, 2445
HpyF3I CTNAG 6 cut(s) 66, 1133, 1157, 1481, 1556, 1902
HpySE526I ACGT 2 cut(s) 448, 1069
Hsp92II CATG 5 cut(s) 386, 470, 526, 1340, 1840
HspAI GCGC 1 cut(s) 120
KspAI GTTAAC 2 cut(s) 24, 537
LweI GCATC 5 cut(s) 46, 1226, 1714, 1736, 1790
MaeI CTAG 2 cut(s) 290, 1679
MaeII ACGT 2 cut(s) 448, 1069
MaeIII GTNAC 4 cut(s) 277, 1526, 1543, 2237
MboII GAAGA 7 cut(s) 482, 590, 1051, 1175, 1571, 2115, 2162
MfeI CAATTG 2 cut(s) 1122, 1771
MflI RGATCY 4 cut(s) 341, 436, 871, 1219
MhlI GDGCHC 1 cut(s) 2019
MlyI GAGTC 2 cut(s) 979, 2310
MmeI TCCRAC 3 cut(s) 384, 1036, 1635
MnlI CCTC 9 cut(s) 175, 512, 644, 851, 1099, 1282, 1352, 2264, 2374
Mph1103I ATGCAT 2 cut(s) 384, 1729
MroXI GAANNNNTTC 4 cut(s) 414, 508, 1832, 1869
MslI CAYNNNNRTG 2 cut(s) 527, 1920
MspI CCGG 1 cut(s) 2408
MspR9I CCNGG 3 cut(s) 441, 1887, 2277
MunI CAATTG 2 cut(s) 1122, 1771
Mva1269I GAATGC 2 cut(s) 1814, 1832
MvaI CCWGG 3 cut(s) 441, 1887, 2277
MwoI GCNNNNNNNGC 6 cut(s) 388, 1606, 2281, 2326, 2436, 2445
NlaIII CATG 5 cut(s) 386, 470, 526, 1340, 1840
NmuCI GTSAC 2 cut(s) 277, 1543
NsiI ATGCAT 2 cut(s) 384, 1729
NspV TTCGAA 2 cut(s) 708, 1961
PagI TCATGA 1 cut(s) 466
PaqCI CACCTGC 1 cut(s) 2275
PctI GAATGC 2 cut(s) 1814, 1832
PdmI GAANNNNTTC 4 cut(s) 414, 508, 1832, 1869
PfeI GAWTC 8 cut(s) 509, 660, 856, 900, 943, 1042, 1552, 1848
PfoI TCCNGGA 1 cut(s) 1885
PleI GAGTC 2 cut(s) 978, 2309
PpsI GAGTC 2 cut(s) 978, 2309
PshBI ATTAAT 1 cut(s) 1010
Psp1406I AACGTT 1 cut(s) 1069
Psp6I CCWGG 3 cut(s) 439, 1885, 2275
PspEI GGTNACC 1 cut(s) 1543
PspFI CCCAGC 1 cut(s) 405
PspGI CCWGG 3 cut(s) 439, 1885, 2275
PspPI GGNCC 3 cut(s) 619, 1657, 2273
PstI CTGCAG 2 cut(s) 1169, 2286
PsuI RGATCY 4 cut(s) 341, 436, 871, 1219
RsaI GTAC 4 cut(s) 186, 1192, 1702, 2218
RsaNI GTAC 4 cut(s) 185, 1191, 1701, 2217
RseI CAYNNNNRTG 2 cut(s) 527, 1920
Sau96I GGNCC 3 cut(s) 619, 1657, 2273
SchI GAGTC 2 cut(s) 979, 2310
ScrFI CCNGG 3 cut(s) 441, 1887, 2277
SduI GDGCHC 1 cut(s) 2019
SfaNI GCATC 5 cut(s) 46, 1226, 1714, 1736, 1790
SfcI CTRYAG 3 cut(s) 432, 1165, 2282
SfuI TTCGAA 2 cut(s) 708, 1961
SinI GGWCC 2 cut(s) 619, 1657
SmiMI CAYNNNNRTG 2 cut(s) 527, 1920
SmlI CTYRAG 4 cut(s) 655, 860, 965, 1610
SmoI CTYRAG 4 cut(s) 655, 860, 965, 1610
SsiI CCGC 1 cut(s) 1438
SspI AATATT 3 cut(s) 231, 296, 1897
SspMI CTAG 2 cut(s) 290, 1679
StyD4I CCNGG 3 cut(s) 439, 1885, 2275
StyI CCWWGG 1 cut(s) 913
TaaI ACNGT 7 cut(s) 50, 375, 426, 1283, 1970, 2237, 2243
TaiI ACGT 2 cut(s) 451, 1072
TaqI TCGA 4 cut(s) 708, 817, 1961, 2097
TatI WGTACW 1 cut(s) 1700
TfiI GAWTC 8 cut(s) 509, 660, 856, 900, 943, 1042, 1552, 1848
TscAI CASTG 4 cut(s) 1122, 1713, 2431, 2437
TseFI GTSAC 2 cut(s) 277, 1543
Tsp45I GTSAC 2 cut(s) 277, 1543
TspDTI ATGAA 7 cut(s) 141, 483, 732, 787, 1756, 1825, 2061
TspGWI ACGGA 4 cut(s) 41, 66, 1007, 2294
TspRI CASTG 4 cut(s) 1122, 1713, 2431, 2437
VpaK11BI GGWCC 2 cut(s) 619, 1657
VspI ATTAAT 1 cut(s) 1010
XapI RAATTY 8 cut(s) 580, 703, 778, 889, 1520, 1732, 2048, 2153
XcmI CCANNNNNNNNNTGG 2 cut(s) 911, 1922
XmiI GTMKAC 1 cut(s) 1675
XmnI GAANNNNTTC 4 cut(s) 414, 508, 1832, 1869
XspI CTAG 2 cut(s) 290, 1679
Zsp2I ATGCAT 2 cut(s) 384, 1729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.