RLG00000015500

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000105
Physical Location & Seq
Reverse (-)
66848850 .. 66852994
4145 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015500

Sequence Viewer

Length: 2748 bp
ATGACTACCCAAGCAGATATTCCTTCTTCTTCTGCTCCTCTCCCTCCGATCCCTAAATACGGTGTCTTTCTTAGTTCCAGCGGAGAAGACACCGATCAAAATTTTACAGATCATCTCTGTACTGCTCTGAACCGGGAGGGGATATTGACATTTAGAGATGGTGAAGAACTCGAGAGGGGAAAAGCAATTGAAGAATCAAGATTTGCAATTGTGGTTTTTTCAGGAAAGTACACCAATCCATGGTGTTTGGATGCGATTGAAAAGATCGTTGAATGCAAGGAAGTGATGAAACTGACAGTACTCCCAGTCTTTCATTATGTGGATCCATCTGATGTACGAAAACAAACAGGGGATCATTTCGGGGAAGCCTTCTCGAAGCATGAAGAGGCCTTTAAACACGAACCGGAGAAGGTGCGCAGGTGGAGACGTGCTCTGACTTCAGCAGCCAATCTCTCTGGATATCATGTGCAAAATGGTCATGAAGCCAAAGCTATTCAAGGAATGACAGAATTTATTCTCAGTGAATTAAATCCTAGAATCCCAAGTGTACCTGACGGATTAGTTGGGATGGATTCTCACCTGAACGAAATGCTCTCGTACTTGGAAGTGGGGTGTCCTGGAGTTCGTACCATAGGGATTTGGGGGATGGGGGGTATCGGAAAGACAACTATTGCAAAAGCTGTTTATGAAAGGATTTATTCTCAGTTTGAAGGTCACGCCTTTCTTGAGAATGTCAGAGAATCAAGTTATAAGCCAGGTGGCAGGGTTGGTTTACAAGAGGAACTTCTTTCGAACTTGCTGAATTGTAATGTAAATGTATCTGACATAGATAGGGGAATAAGCATGATCAAGCATAGACTACATACTAGAATGGTTCTTATCGTTCTTGATGATGTGGATGAACCAGAACAATTGGAAGCATTGTGTGGTGATTGTAGTTGGTTTGGGTCAGGGAGTAGAATCATCATAACAACAAGAGATGAGCGTTTGCTGTTATTATTTGGAGTGGAGAACATATACAAGGTCAATGCATTATCTGATGTTGAGGCTCTTCAGCTGTTTTGTTCGAGAGCCTTCAAGAAGGACCAGGTTGGAGAAGATTTTCTCAAGCTTTCTAAGGAGTATGTAAAATATGCTGCTGGCCTTCCATTAGCTCTTCAAGTATTGGCTGCGTCTCTCCGTGGTATGAAAAAAGATATGTGGTTAAGTGCATTGGCGATACTAAAACAGAAACCTGATAGACAAATTATGGATGTGCTTAAAGTAAGTTATGATGGACTAGATGACATAGATAAGGCTATTTTTTTAGACATTGCATGCTTCCTTAAAGGAGAGAGCGTGGATCGGGTAGGAAGACTATGGGAGTTCTCTATAATCAGTCGTATAAGTGTTCTTCTGGAAAAATCTCTAGTAACTTTGGTTGGAAGAAAATTATGGATGCATGACTTGCTACAACAATTGGGTGAGACAATTGTTTTCCAAGAATGTCCTGAAGAGCCTGGCAATCGTAGCAGGTTGTGGCTTCCGCAAGAAATCATCCATGTACTTGAGAAGAATAAGGGTTCAAGTGCACTTCGAGCGATAGCCTTAGACTTTCCAAAAGTAGAAGAGATACACTGCCACTCAGAGGCATTCTCTGACATGCCCAATTTGACAGACGTCAATTTAAGCTACTCTTGTAAGTTGACCGGGACCCCAGATTTTACATGCGTTTCAAGGCTTGAAAGATTAATTCTTGTAGGTTGTACAAGTTTGGTGGAGATCCATCCATCCATTGGATTTCTTAAAATGCTCACTTGCTTGAATCTTAAAGATTGCAAAAGTCTTAAAATTCTTCCAAGTAAGCTTGAAACGAAAAGCCTTCAAATTCTTGTTCTTTCAGGCTGTTCAAAAGTCAAGAAAATTCCAGATTTTGTGGGATCTATTGAATGCTTGTTAGAACTTTACTTCGATGAGACTGCTATAAAGCAATTACCTATATCAGTTCGACTTCTGACTGGCCTCACTTTATTAAATTTGAGAGATTGCAAAAATCTCAAGAGTCTTCCAAGTGACATTGGCAATTTAAAGTCCCTCAAGCGTCTCGATATCTTTGGATGCTCAAAACTTGAAGCACTACCAGACAGCTTGGGAAAGATAGACTGCTTGGAACAGCTTGATGCTGGTGATAGTTCCATAGGCAATATACCGTCCTCTATTTCCCGTTTGGAAAATCTTGAAGTGTTATCTCTTTGTGGATGTAAAGCGATGTCAGGCAACATGACAAGATATCCACTTAGTTTGCTGGTGCACAAAAGTGATTCAGGTGGTTTGCGTTCTTTAACGGAGCTAAATCTCAGTGACTGTGACCTTCAGGACGGAAGCATACCGGAAGATTTTGGCTGCTTATTCCCTTTAGTTTCACTAAATCTAAGCAAAAACAATTTTGTTAGTCTTCCAAAAAGCATCAGACAGCTTTCAAAGCTTCGAAACTTGAATTTGGAAAGTTGTAAGACTCTTCAAAAATTGCCAGACCTTTCATCAAGCATATACTTCAGTTTCGGGGCAGAGGGTGGCATTTCACAGGAAAGGCTGTCATCAAGTTTCAATTTGATTAATTGCTCCATATTGGTTATCAATCAACGATGCAACAATATAGCATTGAAAATGCTATGGAGGTTCCTTCAGTTCTTCCAGTTCCAGCTAATCGACTTGGTAAATGGAAGTTTGGGACCCATAGCACTGCAAATGGTCTTCGATGCGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 LRR_8 NB-ARC TIR

Protein Analysis

916

Amino Acids

101.94

Weight (kDa)

5.9

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 19 - 182 1.5e-36 TIR domain
TIR_2 PF13676 22 - 109 1.6e-06 TIR domain
NB-ARC PF00931 207 - 362 6.1e-20 NB-ARC domain
WHD_ROQ1 PF23282 431 - 492 1.6e-09 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 587 - 700 1.6e-08 Leucine-rich repeat region
LRR_13 PF23286 620 - 703 8.8e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 704 - 839 8.8e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 750
AarI CACCTGC 1 cut(s) 408
AatII GACGTC 1 cut(s) 1662
Acc16I TGCGCA 1 cut(s) 416
Acc36I ACCTGC 2 cut(s) 408, 1503
AccB7I CCANNNNNTGG 2 cut(s) 240, 1775
AciI CCGC 2 cut(s) 81, 1526
AclWI GGATC 7 cut(s) 43, 317, 330, 360, 1350, 1756, 1927
AcsI RAATTY 7 cut(s) 100, 509, 1830, 1866, 1902, 2014, 2476
AcuI CTGAAG 6 cut(s) 423, 1037, 1512, 2336, 2518, 2648
AcyI GRCGYC 1 cut(s) 1659
AfaI GTAC 9 cut(s) 121, 230, 300, 336, 549, 599, 628, 1545, 1747
AfiI CCNNNNNNNGG 4 cut(s) 59, 240, 1627, 1775
AjiI CACGTC 1 cut(s) 428
AjnI CCWGG 4 cut(s) 616, 754, 1086, 1498
AjuI GAANNNNNNNTTGG 1 cut(s) 35
AleI CACNNNNGTG 1 cut(s) 2295
Alw21I GWGCWC 3 cut(s) 433, 1573, 2292
Alw26I GTCTC 5 cut(s) 418, 1179, 1460, 1949, 2087
Alw44I GTGCAC 2 cut(s) 1569, 2288
AlwI GGATC 7 cut(s) 43, 317, 330, 360, 1350, 1756, 1927
AlwNI CAGNNNCTG 1 cut(s) 2343
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 3 cut(s) 387, 1141, 1999
ApaLI GTGCAC 2 cut(s) 1569, 2288
ApeKI GCWGC 4 cut(s) 443, 1136, 1169, 2382
ApoI RAATTY 7 cut(s) 100, 509, 1830, 1866, 1902, 2014, 2476
AseI ATTAAT 2 cut(s) 1730, 2595
Asp700I GAANNNNTTC 2 cut(s) 513, 1101
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 3 cut(s) 1084, 1692, 2711
AsuC2I CCSGG 2 cut(s) 134, 1690
AsuHPI GGTGA 5 cut(s) 173, 569, 941, 1475, 2177
AsuII TTCGAA 2 cut(s) 791, 2467
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 3 cut(s) 1084, 1692, 2711
BaeGI GKGCMC 2 cut(s) 1573, 2292
BamHI GGATCC 1 cut(s) 322
BbsI GAAGAC 5 cut(s) 93, 1360, 2036, 2426, 2725
Bbv12I GWGCWC 3 cut(s) 433, 1573, 2292
BbvI GCAGC 4 cut(s) 455, 1123, 1156, 2369
BccI CCATC 7 cut(s) 152, 334, 562, 640, 1268, 1773, 1777
BcgI CGANNNNNNTGC 2 cut(s) 1940, 1974
BciT130I CCWGG 4 cut(s) 618, 756, 1088, 1500
BclI TGATCA 1 cut(s) 846
BcnI CCSGG 2 cut(s) 134, 1690
BcoDI GTCTC 5 cut(s) 418, 1179, 1460, 1949, 2087
BfaI CTAG 4 cut(s) 534, 867, 1280, 1409
BfuAI ACCTGC 2 cut(s) 408, 1503
BisI GCNGC 4 cut(s) 444, 1137, 1170, 2383
BlsI GCNGC 4 cut(s) 445, 1138, 1171, 2384
BmcAI AGTACT 1 cut(s) 300
Bme1390I CCNGG 6 cut(s) 134, 618, 756, 1088, 1500, 1690
Bme18I GGWCC 3 cut(s) 1084, 1692, 2711
BmeT110I CYCGRG 1 cut(s) 170
BmgBI CACGTC 1 cut(s) 428
BmgT120I GGNCC 3 cut(s) 1084, 1692, 2711
BmiI GGNNCC 6 cut(s) 324, 1693, 1694, 2660, 2712, 2713
BmrFI CCNGG 6 cut(s) 134, 618, 756, 1088, 1500, 1690
BmrI ACTGGG 1 cut(s) 299
BmsI GCATC 7 cut(s) 241, 1428, 2087, 2149, 2454, 2615, 2728
BmuI ACTGGG 1 cut(s) 299
BoxI GACNNNNGTC 1 cut(s) 1658
BpiI GAAGAC 5 cut(s) 93, 1360, 2036, 2426, 2725
BplI GAGNNNNNCTC 4 cut(s) 415, 447, 1619, 1651
BpmI CTGGAG 1 cut(s) 639
Bpu14I TTCGAA 2 cut(s) 791, 2467
BpuEI CTTGAG 5 cut(s) 746, 1091, 1568, 2021, 2060
BpuMI CCSGG 2 cut(s) 134, 1690
BsaHI GRCGYC 1 cut(s) 1659
BsaJI CCNNGG 2 cut(s) 239, 1180
BsaWI WCCGGW 2 cut(s) 403, 2368
BsaXI ACNNNNNCTCC 2 cut(s) 1323, 1353
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 240, 1627, 1775
Bse1I ACTGG 3 cut(s) 305, 2002, 2674
Bse3DI GCAATG 1 cut(s) 1311
BseBI CCWGG 4 cut(s) 618, 756, 1088, 1500
BseDI CCNNGG 2 cut(s) 239, 1180
BseLI CCNNNNNNNGG 4 cut(s) 59, 240, 1627, 1775
BseMI GCAATG 1 cut(s) 1311
BseMII CTCAG 4 cut(s) 532, 716, 1638, 2350
BseNI ACTGG 3 cut(s) 305, 2002, 2674
BseRI GAGGAG 1 cut(s) 27
BseSI GKGCMC 2 cut(s) 1573, 2292
BseXI GCAGC 4 cut(s) 455, 1123, 1156, 2369
BshFI GGCC 3 cut(s) 389, 1143, 2001
BsiHKAI GWGCWC 3 cut(s) 433, 1573, 2292
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 4 cut(s) 133, 404, 1689, 2369
BslFI GGGAC 3 cut(s) 1705, 2056, 2724
BslI CCNNNNNNNGG 4 cut(s) 59, 240, 1627, 1775
BsmAI GTCTC 5 cut(s) 418, 1179, 1460, 1949, 2087
BsmBI CGTCTC 3 cut(s) 418, 1179, 2087
BsmFI GGGAC 3 cut(s) 1705, 2056, 2724
BsmI GAATGC 3 cut(s) 278, 1631, 1934
BsnI GGCC 3 cut(s) 389, 1143, 2001
BsoBI CYCGRG 1 cut(s) 170
Bsp119I TTCGAA 2 cut(s) 791, 2467
Bsp1286I GDGCHC 3 cut(s) 433, 1573, 2292
Bsp1407I TGTACA 1 cut(s) 1745
Bsp19I CCATGG 1 cut(s) 239
BspACI CCGC 2 cut(s) 81, 1526
BspANI GGCC 3 cut(s) 389, 1143, 2001
BspCNI CTCAG 4 cut(s) 531, 715, 1637, 2349
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 6 cut(s) 324, 1693, 1694, 2660, 2712, 2713
BspMI ACCTGC 2 cut(s) 408, 1503
BspPI GGATC 7 cut(s) 43, 317, 330, 360, 1350, 1756, 1927
BspQI GCTCTTC 3 cut(s) 1056, 1161, 1488
BspT104I TTCGAA 2 cut(s) 791, 2467
BsrDI GCAATG 1 cut(s) 1311
BsrGI TGTACA 1 cut(s) 1745
BsrI ACTGG 3 cut(s) 305, 2002, 2674
BssECI CCNNGG 2 cut(s) 239, 1180
BssNI GRCGYC 1 cut(s) 1659
BssT1I CCWWGG 1 cut(s) 239
Bst2UI CCWGG 4 cut(s) 618, 756, 1088, 1500
Bst4CI ACNGT 4 cut(s) 62, 298, 2190, 2345
Bst6I CTCTTC 6 cut(s) 378, 1056, 1161, 1488, 1602, 2502
BstACI GRCGYC 1 cut(s) 1659
BstAPI GCANNNNNTGC 1 cut(s) 1447
BstAUI TGTACA 1 cut(s) 1745
BstBI TTCGAA 2 cut(s) 791, 2467
BstC8I GCNNGC 2 cut(s) 1141, 1318
BstDEI CTNAG 9 cut(s) 71, 518, 702, 1116, 1588, 1624, 2276, 2336, 2411
BstDSI CCRYGG 2 cut(s) 239, 1180
BstHHI GCGC 1 cut(s) 417
BstMAI GTCTC 5 cut(s) 418, 1179, 1460, 1949, 2087
BstMWI GCNNNNNNNGC 4 cut(s) 1447, 1509, 1577, 2461
BstNI CCWGG 4 cut(s) 618, 756, 1088, 1500
BstNSI RCATGY 3 cut(s) 1320, 1645, 1710
BstPAI GACNNNNGTC 1 cut(s) 1658
BstSCI CCNGG 6 cut(s) 132, 616, 754, 1086, 1498, 1688
BstSLI GKGCMC 2 cut(s) 1573, 2292
BstV1I GCAGC 4 cut(s) 455, 1123, 1156, 2369
BstV2I GAAGAC 5 cut(s) 93, 1360, 2036, 2426, 2725
BstX2I RGATCY 3 cut(s) 322, 1761, 1919
BstYI RGATCY 3 cut(s) 322, 1761, 1919
BsuRI GGCC 3 cut(s) 389, 1143, 2001
BtgI CCRYGG 2 cut(s) 239, 1180
BtgZI GCGATG 1 cut(s) 2261
BtrI CACGTC 1 cut(s) 428
BtsI GCAGTG 2 cut(s) 1615, 2720
BtsIMutI CAGTG 4 cut(s) 526, 1615, 2344, 2720
BveI ACCTGC 2 cut(s) 408, 1503
Cac8I GCNNGC 2 cut(s) 1141, 1318
CaiI CAGNNNCTG 1 cut(s) 2343
CciI TCATGA 1 cut(s) 478
CfoI GCGC 1 cut(s) 417
Cfr13I GGNCC 3 cut(s) 1084, 1692, 2711
CseI GACGC 2 cut(s) 1161, 2069
CsiI ACCWGGT 1 cut(s) 1086
Csp6I GTAC 9 cut(s) 120, 229, 299, 335, 548, 598, 627, 1544, 1746
CspCI CAANNNNNGTGG 4 cut(s) 1737, 1772, 2262, 2297
CviQI GTAC 9 cut(s) 120, 229, 299, 335, 548, 598, 627, 1544, 1746
DdeI CTNAG 9 cut(s) 71, 518, 702, 1116, 1588, 1624, 2276, 2336, 2411
DraI TTTAAA 2 cut(s) 394, 2067
Eam1104I CTCTTC 6 cut(s) 378, 1056, 1161, 1488, 1602, 2502
EarI CTCTTC 6 cut(s) 378, 1056, 1161, 1488, 1602, 2502
Eco130I CCWWGG 1 cut(s) 239
Eco147I AGGCCT 1 cut(s) 389
Eco32I GATATC 3 cut(s) 461, 2089, 2270
Eco47I GGWCC 3 cut(s) 1084, 1692, 2711
Eco57I CTGAAG 6 cut(s) 423, 1037, 1512, 2336, 2518, 2648
Eco88I CYCGRG 1 cut(s) 170
EcoO109I RGGNCCY 2 cut(s) 1692, 2711
EcoRII CCWGG 4 cut(s) 616, 754, 1086, 1498
EcoRV GATATC 3 cut(s) 461, 2089, 2270
EcoT14I CCWWGG 1 cut(s) 239
EcoT22I ATGCAT 2 cut(s) 1033, 1443
ErhI CCWWGG 1 cut(s) 239
Esp3I CGTCTC 3 cut(s) 418, 1179, 2087
FalI AAGNNNNNCTT 4 cut(s) 768, 800, 1660, 1692
FaqI GGGAC 3 cut(s) 1705, 2056, 2724
FbaI TGATCA 1 cut(s) 846
Fnu4HI GCNGC 4 cut(s) 444, 1137, 1170, 2383
Fsp4HI GCNGC 4 cut(s) 444, 1137, 1170, 2383
FspBI CTAG 4 cut(s) 534, 867, 1280, 1409
FspI TGCGCA 1 cut(s) 416
GlaI GCGC 1 cut(s) 416
GluI GCNGC 4 cut(s) 444, 1137, 1170, 2383
GsuI CTGGAG 1 cut(s) 639
HaeIII GGCC 3 cut(s) 389, 1143, 2001
HapII CCGG 4 cut(s) 133, 404, 1689, 2369
HgaI GACGC 2 cut(s) 1161, 2069
HhaI GCGC 1 cut(s) 417
Hin1I GRCGYC 1 cut(s) 1659
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HincII GTYRAC 1 cut(s) 1686
HindII GTYRAC 1 cut(s) 1686
HindIII AAGCTT 3 cut(s) 1109, 1844, 2462
HinfI GANTC 9 cut(s) 194, 537, 572, 740, 960, 1804, 2041, 2300, 2494
HpaII CCGG 4 cut(s) 133, 404, 1689, 2369
HphI GGTGA 5 cut(s) 173, 569, 941, 1475, 2177
Hpy166II GTNNAC 6 cut(s) 231, 548, 773, 1571, 1686, 2290
Hpy8I GTNNAC 6 cut(s) 231, 548, 773, 1571, 1686, 2290
HpyCH4III ACNGT 4 cut(s) 62, 298, 2190, 2345
HpyCH4IV ACGT 2 cut(s) 427, 1659
HpyF10VI GCNNNNNNNGC 4 cut(s) 1447, 1509, 1577, 2461
HpyF3I CTNAG 9 cut(s) 71, 518, 702, 1116, 1588, 1624, 2276, 2336, 2411
HpySE526I ACGT 2 cut(s) 427, 1659
Hsp92I GRCGYC 1 cut(s) 1659
HspAI GCGC 1 cut(s) 415
KflI GGGWCCC 2 cut(s) 1692, 2711
Ksp22I TGATCA 1 cut(s) 846
LguI GCTCTTC 3 cut(s) 1056, 1161, 1488
LmnI GCTCC 3 cut(s) 40, 2326, 2606
Lsp1109I GCAGC 4 cut(s) 455, 1123, 1156, 2369
LweI GCATC 7 cut(s) 241, 1428, 2087, 2149, 2454, 2615, 2728
MabI ACCWGGT 1 cut(s) 1086
MaeI CTAG 4 cut(s) 534, 867, 1280, 1409
MaeII ACGT 2 cut(s) 427, 1659
MaeIII GTNAC 5 cut(s) 713, 1411, 2051, 2339, 2345
MfeI CAATTG 5 cut(s) 186, 207, 911, 1457, 1470
MflI RGATCY 3 cut(s) 322, 1761, 1919
MhlI GDGCHC 3 cut(s) 433, 1573, 2292
MlyI GAGTC 2 cut(s) 2050, 2488
MmeI TCCRAC 2 cut(s) 1072, 1402
Mph1103I ATGCAT 2 cut(s) 1033, 1443
MroXI GAANNNNTTC 2 cut(s) 513, 1101
MslI CAYNNNNRTG 1 cut(s) 2295
MspA1I CMGCKG 2 cut(s) 81, 1057
MspI CCGG 4 cut(s) 133, 404, 1689, 2369
MspR9I CCNGG 6 cut(s) 134, 618, 756, 1088, 1500, 1690
MunI CAATTG 5 cut(s) 186, 207, 911, 1457, 1470
Mva1269I GAATGC 3 cut(s) 278, 1631, 1934
MvaI CCWGG 4 cut(s) 618, 756, 1088, 1500
MwoI GCNNNNNNNGC 4 cut(s) 1447, 1509, 1577, 2461
NciI CCSGG 2 cut(s) 134, 1690
NcoI CCATGG 1 cut(s) 239
NlaIV GGNNCC 6 cut(s) 324, 1693, 1694, 2660, 2712, 2713
NmuCI GTSAC 4 cut(s) 713, 2051, 2339, 2345
NsbI TGCGCA 1 cut(s) 416
NsiI ATGCAT 2 cut(s) 1033, 1443
NspI RCATGY 3 cut(s) 1320, 1645, 1710
NspV TTCGAA 2 cut(s) 791, 2467
OliI CACNNNNGTG 1 cut(s) 2295
PaeI GCATGC 1 cut(s) 1320
PaeR7I CTCGAG 1 cut(s) 170
PagI TCATGA 1 cut(s) 478
PaqCI CACCTGC 1 cut(s) 408
PceI AGGCCT 1 cut(s) 389
PciSI GCTCTTC 3 cut(s) 1056, 1161, 1488
PctI GAATGC 3 cut(s) 278, 1631, 1934
PdmI GAANNNNTTC 2 cut(s) 513, 1101
PfeI GAWTC 7 cut(s) 194, 537, 572, 740, 960, 1804, 2300
PflMI CCANNNNNTGG 2 cut(s) 240, 1775
PfoI TCCNGGA 1 cut(s) 616
PkrI GCNGC 4 cut(s) 445, 1138, 1171, 2384
PleI GAGTC 2 cut(s) 2049, 2488
PpsI GAGTC 2 cut(s) 2049, 2488
PpuMI RGGWCCY 2 cut(s) 1692, 2711
PshAI GACNNNNGTC 1 cut(s) 1658
PshBI ATTAAT 2 cut(s) 1730, 2595
PsiI TTATAA 1 cut(s) 750
Psp5II RGGWCCY 2 cut(s) 1692, 2711
Psp6I CCWGG 4 cut(s) 616, 754, 1086, 1498
PspGI CCWGG 4 cut(s) 616, 754, 1086, 1498
PspN4I GGNNCC 6 cut(s) 324, 1693, 1694, 2660, 2712, 2713
PspPI GGNCC 3 cut(s) 1084, 1692, 2711
PspPPI RGGWCCY 2 cut(s) 1692, 2711
PstNI CAGNNNCTG 1 cut(s) 2343
PsuI RGATCY 3 cut(s) 322, 1761, 1919
PvuII CAGCTG 1 cut(s) 1057
RsaI GTAC 9 cut(s) 121, 230, 300, 336, 549, 599, 628, 1545, 1747
RsaNI GTAC 9 cut(s) 120, 229, 299, 335, 548, 598, 627, 1544, 1746
RseI CAYNNNNRTG 1 cut(s) 2295
SapI GCTCTTC 3 cut(s) 1056, 1161, 1488
SatI GCNGC 4 cut(s) 444, 1137, 1170, 2383
Sau96I GGNCC 3 cut(s) 1084, 1692, 2711
ScaI AGTACT 1 cut(s) 300
SchI GAGTC 2 cut(s) 2050, 2488
ScrFI CCNGG 6 cut(s) 134, 618, 756, 1088, 1500, 1690
SduI GDGCHC 3 cut(s) 433, 1573, 2292
SexAI ACCWGGT 1 cut(s) 1086
SfaNI GCATC 7 cut(s) 241, 1428, 2087, 2149, 2454, 2615, 2728
Sfr274I CTCGAG 1 cut(s) 170
SfuI TTCGAA 2 cut(s) 791, 2467
SinI GGWCC 3 cut(s) 1084, 1692, 2711
SlaI CTCGAG 1 cut(s) 170
SmiMI CAYNNNNRTG 1 cut(s) 2295
SmlI CTYRAG 6 cut(s) 170, 725, 1106, 1547, 2036, 2075
SmoI CTYRAG 6 cut(s) 170, 725, 1106, 1547, 2036, 2075
SphI GCATGC 1 cut(s) 1320
SseBI AGGCCT 1 cut(s) 389
SsiI CCGC 2 cut(s) 81, 1526
SspMI CTAG 4 cut(s) 534, 867, 1280, 1409
StuI AGGCCT 1 cut(s) 389
StyD4I CCNGG 6 cut(s) 132, 616, 754, 1086, 1498, 1688
StyI CCWWGG 1 cut(s) 239
TaaI ACNGT 4 cut(s) 62, 298, 2190, 2345
TaiI ACGT 2 cut(s) 430, 1662
TatI WGTACW 5 cut(s) 119, 228, 298, 1543, 1745
TfiI GAWTC 7 cut(s) 194, 537, 572, 740, 960, 1804, 2300
TscAI CASTG 4 cut(s) 526, 1622, 2344, 2727
TseFI GTSAC 4 cut(s) 713, 2051, 2339, 2345
TseI GCWGC 4 cut(s) 443, 1136, 1169, 2382
Tsp45I GTSAC 4 cut(s) 713, 2051, 2339, 2345
TspDTI ATGAA 8 cut(s) 302, 302, 396, 495, 702, 915, 1202, 2508
TspGWI ACGGA 4 cut(s) 570, 1169, 2339, 2373
TspRI CASTG 4 cut(s) 526, 1622, 2344, 2727
Van91I CCANNNNNTGG 2 cut(s) 240, 1775
VneI GTGCAC 2 cut(s) 1569, 2288
VpaK11BI GGWCC 3 cut(s) 1084, 1692, 2711
VspI ATTAAT 2 cut(s) 1730, 2595
XapI RAATTY 7 cut(s) 100, 509, 1830, 1866, 1902, 2014, 2476
XceI RCATGY 3 cut(s) 1320, 1645, 1710
XcmI CCANNNNNNNNNTGG 1 cut(s) 1772
XhoI CTCGAG 1 cut(s) 170
XmnI GAANNNNTTC 2 cut(s) 513, 1101
XspI CTAG 4 cut(s) 534, 867, 1280, 1409
ZraI GACGTC 1 cut(s) 1660
ZrmI AGTACT 1 cut(s) 300
Zsp2I ATGCAT 2 cut(s) 1033, 1443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.