Rroxscaffold_4G00327330

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000105
Physical Location & Seq
Forward (+)
59976424 .. 59980615
4192 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00327330.1

Sequence Viewer

Length: 2604 bp
ATGACTAGAAAGTGGAAATACGATGTCTTTCTAAGTTTCAGGGGGGAAGATACCCGCTATACTTTTACCATTCATCTGTATTCTGCACTAAAACGCCAAGGAATTCTCGCCTTTATGGATGACCCAGTGCTTGAGAAAGGAAAATCCATTAGGACTGAACTTTTCGGTGCAATTGAGGAGTCTAGATGTGCGATTGTCATTCTCTCAAAGAACTATGCTTCTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCGATGCATGAAAGACATGGGCCAACAAGTCTTCCCGGTCTTCTACTCGGGCGTGGATCCTTCTGATGTGCGGCACCAAAGGCGAAGTTTTGATCTCATCAAATGGGTGCACCAAGTAGATGTGGAAGTAAGGGAACATGAAGAAGTTTATGGGAAGAATGGTGATAGACTAAATGCGTGGAGAGATGCTTTGACTGAAGTGGCCAATCTGTCTGGCTGGGATAACAAGAATTATGAATTTGATACAAGAGTAATTGAGGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCTAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGTGTTGATGATTTAATGGGCAATTACATATGTCCACAGCTTTGTGGGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAATTACAAGTGGCTCATTGGGATCCAGAGGCCTTTTCAAATTTGTCTCAACTCAGTCTTCTCCATATTCATAATGTGGACCTTCCCAAAGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGCAAATTGTCTGTTTTGTGTGGTGGCTCACTGTTTCGATTTTATGAGCAGAATTTTGACAGGTTGAAGTTCATCAAACTCTGTCATTCTCAAAACATTGTGGAGACCCCAGACCTCACAGGCGTTCAGAATCTTGAGAGTTTAGATCTTGAAGGATGTGAGAATTTGGTTAGAATCCATGAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTCAAAGACTGCAAAAATCTCAAGAGTCTGCCAAGAAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAGCTGCTCAAAAGTTAAGAAGATTCCTGAGTTTGTTGGAAATATGGAATGTTTGTCGGTGCTTTATCTTGATGAGACTGCCATTGAGGAACTGCCTGTGTCAATTGAACGGCTGAGTGGCCTTGTGTCATTGAATCTGAGCAAATGCAGAAATCTTGTCTGTCTTCCGATCACCATCAATAAATTGAAGTCTATTAAAAATCTTAATCTTTCTGGATGCTTGAAACTCGGCAAACATCAGGTGAATGTGGGGGAGATAGATTGTTTTGAGGAAAATGATGTGAATAGTGGGTCTGCAATAGAAATGTCATCTTCCCATGCTCTCATAAACTCTGTGAGAGGTTCAATCTTTCATGTATATGAAGTAGTTTGGGGATCTTTAAATAAGTTCTTGCCTTCTGGATTGATGCAAAAAGTGAATGCAGGGCCATTGAGTTTATACTTGCCTATTTCTGGTTTGTGTAATTTAACGTATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTCCCAACGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACCATTTTGTTCGCGTTCCTTCAGGCATTCGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTAAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGACGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAATAGACTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAAGAAAGGATGCAATAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGCTAACAACCTATCTCTTCTTGCACAGGGAATCTCTAATAAGAGGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCCAAGTGGTTCGGCCATCGGAGTGTTGGGTGTTCATTAAGTGTGCCTCTACCTGCAGATTGGAATAACAGTCGGTTTTTAGGATTTGCTTTGTGTGCTGATTTTGTACTCCGTGAGCACCATCGGGTGGATGAGCTTTATATAGATGAATTCAACACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGATCGATGGAAGAGAACTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTGAATCAAATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAGTCAGCTTGAGTTCTTATTTGAAACCAGAGGGCCGGGTCTGAAGGTGAACGAGTGTGGAGTCCGTCTGATATATGAGCAAGAAGTGCAAGGGTTGAACCAAACAACCACTCAATCAAGCAGTAGGATGTCTCCTTATGCGGATATATTGATTGGTTTTGACATTCCAGTTGGAGGGGAAACCAGTGGCACTGGTAGCAGAACTTGCACGCTTGAAGAATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

867

Amino Acids

98.52

Weight (kDa)

6.1

Isoelectric Point (pI)

38.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 6 - 188 2.4e-45 TIR domain
TIR_2 PF13676 9 - 120 3.7e-16 TIR domain
LRR_13 PF23286 362 - 447 1.1e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 373 - 442 9.9e-09 Leucine-rich repeat region
C-JID PF20160 674 - 823 1.7e-18 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2098
AccB1I GGYRCC 1 cut(s) 330
AccB7I CCANNNNNTGG 2 cut(s) 664, 2164
AccI GTMKAC 2 cut(s) 551, 1786
AccII CGCG 1 cut(s) 1689
AciI CCGC 3 cut(s) 55, 328, 2519
AclWI GGATC 5 cut(s) 308, 321, 698, 711, 1492
AcoI YGGCCR 2 cut(s) 459, 2050
AcsI RAATTY 9 cut(s) 102, 494, 721, 868, 979, 1631, 1843, 2186, 2291
AcuI CTGAAG 5 cut(s) 474, 1680, 1958, 2249, 2441
AfaI GTAC 3 cut(s) 1813, 2145, 2356
AfiI CCNNNNNNNGG 4 cut(s) 664, 1562, 2164, 2552
AjnI CCWGG 2 cut(s) 2023, 2298
AjuI GAANNNNNNNTTGG 2 cut(s) 1718, 1750
AluBI AGCT 7 cut(s) 634, 1021, 1113, 1720, 1957, 2173, 2386
AluI AGCT 7 cut(s) 634, 1021, 1113, 1720, 1957, 2173, 2386
Alw21I GWGCWC 2 cut(s) 369, 2157
Alw26I GTCTC 6 cut(s) 732, 793, 914, 1178, 1744, 2514
Alw44I GTGCAC 1 cut(s) 365
AlwI GGATC 5 cut(s) 308, 321, 698, 711, 1492
Ama87I CYCGRG 1 cut(s) 304
AoxI GGCC 8 cut(s) 277, 459, 711, 772, 1228, 1535, 2050, 2411
ApaLI GTGCAC 1 cut(s) 365
ApeKI GCWGC 1 cut(s) 1113
ApoI RAATTY 9 cut(s) 102, 494, 721, 868, 979, 1631, 1843, 2186, 2291
AseI ATTAAT 1 cut(s) 1100
Asp700I GAANNNNTTC 4 cut(s) 589, 649, 1943, 2007
AspS9I GGNCC 4 cut(s) 277, 760, 1535, 2411
AsuC2I CCSGG 2 cut(s) 293, 2415
AsuHPI GGTGA 7 cut(s) 431, 1273, 1363, 1622, 1666, 2306, 2437
AvaI CYCGRG 1 cut(s) 304
AvaII GGWCC 1 cut(s) 760
BaeGI GKGCMC 1 cut(s) 369
BaeI ACNNNNGTAYC 2 cut(s) 492, 525
BalI TGGCCA 1 cut(s) 461
BamHI GGATCC 2 cut(s) 313, 703
BanI GGYRCC 1 cut(s) 330
BauI CACGAG 1 cut(s) 2337
BbsI GAAGAC 5 cut(s) 280, 289, 731, 1265, 1946
Bbv12I GWGCWC 2 cut(s) 369, 2157
BbvI GCAGC 1 cut(s) 1100
BccI CCATC 5 cut(s) 1292, 1783, 2061, 2166, 2231
BceAI ACGGC 1 cut(s) 1235
BciT130I CCWGG 2 cut(s) 2025, 2300
BcnI CCSGG 2 cut(s) 293, 2415
BcoDI GTCTC 6 cut(s) 732, 793, 914, 1178, 1744, 2514
BfaI CTAG 5 cut(s) 6, 183, 561, 1110, 1790
BfmI CTRYAG 1 cut(s) 2091
BfuAI ACCTGC 1 cut(s) 2098
BglII AGATCT 1 cut(s) 961
BisI GCNGC 2 cut(s) 329, 1114
BlsI GCNGC 2 cut(s) 330, 1115
Bme1390I CCNGG 4 cut(s) 293, 2025, 2300, 2415
Bme18I GGWCC 1 cut(s) 760
BmeT110I CYCGRG 1 cut(s) 304
BmgT120I GGNCC 4 cut(s) 277, 760, 1535, 2411
BmiI GGNNCC 3 cut(s) 315, 332, 705
BmrFI CCNGG 4 cut(s) 293, 2025, 2300, 2415
BmrI ACTGGG 1 cut(s) 119
BmsI GCATC 7 cut(s) 251, 433, 1316, 1506, 1825, 1847, 1901
BmuI ACTGGG 1 cut(s) 119
BpiI GAAGAC 5 cut(s) 280, 289, 731, 1265, 1946
BpuEI CTTGAG 6 cut(s) 152, 817, 971, 1040, 1742, 2408
BpuMI CCSGG 2 cut(s) 293, 2415
Bsa29I ATCGAT 1 cut(s) 2235
BsaBI GATNNNNATC 1 cut(s) 1283
BsaI GGTCTC 1 cut(s) 914
BsaJI CCNNGG 3 cut(s) 97, 658, 1003
Bsc4I CCNNNNNNNGG 4 cut(s) 664, 1562, 2164, 2552
Bse1I ACTGG 6 cut(s) 125, 820, 2253, 2546, 2562, 2575
Bse8I GATNNNNATC 1 cut(s) 1283
BseBI CCWGG 2 cut(s) 2025, 2300
BseCI ATCGAT 1 cut(s) 2235
BseDI CCNNGG 3 cut(s) 97, 658, 1003
BseGI GGATG 7 cut(s) 124, 244, 977, 1331, 1916, 2173, 2511
BseJI GATNNNNATC 1 cut(s) 1283
BseLI CCNNNNNNNGG 4 cut(s) 664, 1562, 2164, 2552
BseMII CTCAG 5 cut(s) 748, 1128, 1214, 1238, 1583
BseNI ACTGG 6 cut(s) 125, 820, 2253, 2546, 2562, 2575
BseRI GAGGAG 2 cut(s) 191, 794
BseSI GKGCMC 1 cut(s) 369
BseXI GCAGC 1 cut(s) 1100
BseYI CCCAGC 1 cut(s) 474
BsgI GTGCAG 1 cut(s) 69
Bsh1236I CGCG 1 cut(s) 1689
BshFI GGCC 8 cut(s) 279, 461, 713, 774, 1230, 1537, 2052, 2413
BshNI GGYRCC 1 cut(s) 330
BshVI ATCGAT 1 cut(s) 2235
BsiHKAI GWGCWC 2 cut(s) 369, 2157
BsiHKCI CYCGRG 1 cut(s) 304
BsiSI CCGG 2 cut(s) 293, 2414
BslI CCNNNNNNNGG 4 cut(s) 664, 1562, 2164, 2552
BsmAI GTCTC 6 cut(s) 732, 793, 914, 1178, 1744, 2514
BsmI GAATGC 2 cut(s) 1534, 1701
BsnI GGCC 8 cut(s) 279, 461, 713, 774, 1230, 1537, 2052, 2413
Bso31I GGTCTC 1 cut(s) 914
BsoBI CYCGRG 1 cut(s) 304
Bsp1286I GDGCHC 2 cut(s) 369, 2157
Bsp143I GATC 7 cut(s) 313, 349, 703, 961, 1278, 1484, 2232
BspACI CCGC 3 cut(s) 55, 328, 2519
BspANI GGCC 8 cut(s) 279, 461, 713, 774, 1230, 1537, 2052, 2413
BspCNI CTCAG 5 cut(s) 747, 1129, 1215, 1239, 1584
BspDI ATCGAT 1 cut(s) 2235
BspFNI CGCG 1 cut(s) 1689
BspLI GGNNCC 3 cut(s) 315, 332, 705
BspMAI CTGCAG 1 cut(s) 2095
BspMI ACCTGC 1 cut(s) 2098
BspPI GGATC 5 cut(s) 308, 321, 698, 711, 1492
BspT107I GGYRCC 1 cut(s) 330
BspTNI GGTCTC 1 cut(s) 914
BsrI ACTGG 6 cut(s) 125, 820, 2253, 2546, 2562, 2575
BssECI CCNNGG 3 cut(s) 97, 658, 1003
BssMI GATC 7 cut(s) 313, 349, 703, 961, 1278, 1484, 2232
BssNAI GTATAC 1 cut(s) 552
BssSI CACGAG 1 cut(s) 2337
BssT1I CCWWGG 3 cut(s) 97, 658, 1003
Bst1107I GTATAC 1 cut(s) 552
Bst2BI CACGAG 1 cut(s) 2337
Bst2UI CCWGG 2 cut(s) 2025, 2300
Bst4CI ACNGT 4 cut(s) 849, 1808, 2108, 2375
Bst6I CTCTTC 2 cut(s) 1976, 2236
BstAPI GCANNNNNTGC 2 cut(s) 2464, 2583
BstC8I GCNNGC 2 cut(s) 2271, 2588
BstDEI CTNAG 8 cut(s) 32, 734, 1137, 1223, 1247, 1592, 1667, 1716
BstEII GGTNACC 1 cut(s) 1654
BstF5I GGATG 7 cut(s) 124, 244, 977, 1331, 1916, 2173, 2511
BstFNI CGCG 1 cut(s) 1689
BstKTI GATC 7 cut(s) 316, 352, 706, 964, 1281, 1487, 2235
BstMAI GTCTC 6 cut(s) 732, 793, 914, 1178, 1744, 2514
BstMBI GATC 7 cut(s) 313, 349, 703, 961, 1278, 1484, 2232
BstMWI GCNNNNNNNGC 6 cut(s) 337, 1717, 2132, 2464, 2574, 2583
BstNI CCWGG 2 cut(s) 2025, 2300
BstPI GGTNACC 1 cut(s) 1654
BstSCI CCNGG 4 cut(s) 291, 2023, 2298, 2413
BstSFI CTRYAG 1 cut(s) 2091
BstSLI GKGCMC 1 cut(s) 369
BstUI CGCG 1 cut(s) 1689
BstV1I GCAGC 1 cut(s) 1100
BstV2I GAAGAC 5 cut(s) 280, 289, 731, 1265, 1946
BstX2I RGATCY 4 cut(s) 313, 703, 961, 1484
BstYI RGATCY 4 cut(s) 313, 703, 961, 1484
BstZ17I GTATAC 1 cut(s) 552
Bsu15I ATCGAT 1 cut(s) 2235
BsuRI GGCC 8 cut(s) 279, 461, 713, 774, 1230, 1537, 2052, 2413
BsuTUI ATCGAT 1 cut(s) 2235
BtsCI GGATG 7 cut(s) 124, 244, 977, 1331, 1916, 2173, 2511
BtsIMutI CAGTG 5 cut(s) 132, 845, 1817, 2568, 2569
BveI ACCTGC 1 cut(s) 2098
Cac8I GCNNGC 2 cut(s) 2271, 2588
Cfr13I GGNCC 4 cut(s) 277, 760, 1535, 2411
ClaI ATCGAT 1 cut(s) 2235
Csp6I GTAC 3 cut(s) 1812, 2144, 2355
CviAII CATG 9 cut(s) 228, 265, 274, 395, 664, 995, 1427, 1463, 1948
CviQI GTAC 3 cut(s) 1812, 2144, 2355
DdeI CTNAG 8 cut(s) 32, 734, 1137, 1223, 1247, 1592, 1667, 1716
DpnI GATC 7 cut(s) 315, 351, 705, 963, 1280, 1486, 2234
DpnII GATC 7 cut(s) 313, 349, 703, 961, 1278, 1484, 2232
DraI TTTAAA 1 cut(s) 1491
EaeI YGGCCR 2 cut(s) 459, 2050
Eam1104I CTCTTC 2 cut(s) 1976, 2236
EarI CTCTTC 2 cut(s) 1976, 2236
Eco130I CCWWGG 3 cut(s) 97, 658, 1003
Eco147I AGGCCT 2 cut(s) 713, 774
Eco31I GGTCTC 1 cut(s) 914
Eco47I GGWCC 1 cut(s) 760
Eco57I CTGAAG 5 cut(s) 474, 1680, 1958, 2249, 2441
Eco88I CYCGRG 1 cut(s) 304
Eco91I GGTNACC 1 cut(s) 1654
EcoO65I GGTNACC 1 cut(s) 1654
EcoRI GAATTC 2 cut(s) 102, 2186
EcoRII CCWGG 2 cut(s) 2023, 2298
EcoT14I CCWWGG 3 cut(s) 97, 658, 1003
EcoT22I ATGCAT 2 cut(s) 266, 1840
ErhI CCWWGG 3 cut(s) 97, 658, 1003
FaeI CATG 9 cut(s) 231, 268, 277, 398, 667, 998, 1430, 1466, 1951
FatI CATG 9 cut(s) 227, 264, 273, 394, 663, 994, 1426, 1462, 1947
FauI CCCGC 1 cut(s) 62
FauNDI CATATG 1 cut(s) 623
FblI GTMKAC 2 cut(s) 551, 1786
Fnu4HI GCNGC 2 cut(s) 329, 1114
FokI GGATG 7 cut(s) 131, 251, 984, 1338, 1923, 2180, 2518
Fsp4HI GCNGC 2 cut(s) 329, 1114
FspBI CTAG 5 cut(s) 6, 183, 561, 1110, 1790
GluI GCNGC 2 cut(s) 329, 1114
GsaI CCCAGC 1 cut(s) 478
HaeIII GGCC 8 cut(s) 279, 461, 713, 774, 1230, 1537, 2052, 2413
HapII CCGG 2 cut(s) 293, 2414
Hin1II CATG 9 cut(s) 231, 268, 277, 398, 667, 998, 1430, 1466, 1951
HincII GTYRAC 2 cut(s) 568, 678
HindII GTYRAC 2 cut(s) 568, 678
HindIII AAGCTT 2 cut(s) 1019, 1718
HpaII CCGG 2 cut(s) 293, 2414
HphI GGTGA 7 cut(s) 431, 1273, 1363, 1622, 1666, 2306, 2437
Hpy166II GTNNAC 8 cut(s) 367, 552, 568, 629, 678, 760, 1787, 2428
Hpy188I TCNGA 9 cut(s) 322, 945, 1248, 1278, 1587, 1768, 2058, 2421, 2448
Hpy8I GTNNAC 8 cut(s) 367, 552, 568, 629, 678, 760, 1787, 2428
HpyAV CCTTC 8 cut(s) 327, 773, 962, 1515, 1607, 1704, 1781, 2416
HpyCH4III ACNGT 4 cut(s) 849, 1808, 2108, 2375
HpyCH4IV ACGT 1 cut(s) 1580
HpyF10VI GCNNNNNNNGC 6 cut(s) 337, 1717, 2132, 2464, 2574, 2583
HpyF3I CTNAG 8 cut(s) 32, 734, 1137, 1223, 1247, 1592, 1667, 1716
HpySE526I ACGT 1 cut(s) 1580
Hsp92II CATG 9 cut(s) 231, 268, 277, 398, 667, 998, 1430, 1466, 1951
Kzo9I GATC 7 cut(s) 313, 349, 703, 961, 1278, 1484, 2232
LmnI GCTCC 1 cut(s) 1616
Lsp1109I GCAGC 1 cut(s) 1100
LweI GCATC 7 cut(s) 251, 433, 1316, 1506, 1825, 1847, 1901
MaeI CTAG 5 cut(s) 6, 183, 561, 1110, 1790
MaeII ACGT 1 cut(s) 1580
MaeIII GTNAC 2 cut(s) 1637, 1654
MalI GATC 7 cut(s) 315, 351, 705, 963, 1280, 1486, 2234
MboI GATC 7 cut(s) 313, 349, 703, 961, 1278, 1484, 2232
MfeI CAATTG 3 cut(s) 171, 1212, 1882
MflI RGATCY 4 cut(s) 313, 703, 961, 1484
MhlI GDGCHC 2 cut(s) 369, 2157
MlsI TGGCCA 1 cut(s) 461
MluNI TGGCCA 1 cut(s) 461
MlyI GAGTC 4 cut(s) 188, 795, 1069, 2448
MmeI TCCRAC 2 cut(s) 1126, 2530
Mox20I TGGCCA 1 cut(s) 461
Mph1103I ATGCAT 2 cut(s) 266, 1840
MroXI GAANNNNTTC 4 cut(s) 589, 649, 1943, 2007
MscI TGGCCA 1 cut(s) 461
MslI CAYNNNNRTG 4 cut(s) 584, 668, 1467, 2058
Msp20I TGGCCA 1 cut(s) 461
MspI CCGG 2 cut(s) 293, 2414
MspR9I CCNGG 4 cut(s) 293, 2025, 2300, 2415
MunI CAATTG 3 cut(s) 171, 1212, 1882
Mva1269I GAATGC 2 cut(s) 1534, 1701
MvaI CCWGG 2 cut(s) 2025, 2300
MvnI CGCG 1 cut(s) 1689
MwoI GCNNNNNNNGC 6 cut(s) 337, 1717, 2132, 2464, 2574, 2583
NciI CCSGG 2 cut(s) 293, 2415
NdeI CATATG 1 cut(s) 623
NdeII GATC 7 cut(s) 313, 349, 703, 961, 1278, 1484, 2232
NlaIII CATG 9 cut(s) 231, 268, 277, 398, 667, 998, 1430, 1466, 1951
NlaIV GGNNCC 3 cut(s) 315, 332, 705
NmeAIII GCCGAG 1 cut(s) 1317
NmuCI GTSAC 1 cut(s) 1654
NsiI ATGCAT 2 cut(s) 266, 1840
PceI AGGCCT 2 cut(s) 713, 774
PctI GAATGC 2 cut(s) 1534, 1701
PdmI GAANNNNTTC 4 cut(s) 589, 649, 1943, 2007
PflMI CCANNNNNTGG 2 cut(s) 664, 2164
PfoI TCCNGGA 1 cut(s) 2023
PkrI GCNGC 2 cut(s) 330, 1115
PleI GAGTC 4 cut(s) 187, 795, 1068, 2447
PpsI GAGTC 4 cut(s) 187, 795, 1068, 2447
PshBI ATTAAT 1 cut(s) 1100
Psp6I CCWGG 2 cut(s) 2023, 2298
PspEI GGTNACC 1 cut(s) 1654
PspFI CCCAGC 1 cut(s) 474
PspGI CCWGG 2 cut(s) 2023, 2298
PspN4I GGNNCC 3 cut(s) 315, 332, 705
PspPI GGNCC 4 cut(s) 277, 760, 1535, 2411
PstI CTGCAG 1 cut(s) 2095
PsuI RGATCY 4 cut(s) 313, 703, 961, 1484
RsaI GTAC 3 cut(s) 1813, 2145, 2356
RsaNI GTAC 3 cut(s) 1812, 2144, 2355
RseI CAYNNNNRTG 4 cut(s) 584, 668, 1467, 2058
SatI GCNGC 2 cut(s) 329, 1114
Sau3AI GATC 7 cut(s) 313, 349, 703, 961, 1278, 1484, 2232
Sau96I GGNCC 4 cut(s) 277, 760, 1535, 2411
SchI GAGTC 4 cut(s) 188, 795, 1069, 2448
ScrFI CCNGG 4 cut(s) 293, 2025, 2300, 2415
SduI GDGCHC 2 cut(s) 369, 2157
SfaNI GCATC 7 cut(s) 251, 433, 1316, 1506, 1825, 1847, 1901
SfcI CTRYAG 1 cut(s) 2091
SinI GGWCC 1 cut(s) 760
SmiMI CAYNNNNRTG 4 cut(s) 584, 668, 1467, 2058
SmlI CTYRAG 6 cut(s) 131, 796, 950, 1055, 1721, 2387
SmoI CTYRAG 6 cut(s) 131, 796, 950, 1055, 1721, 2387
SseBI AGGCCT 2 cut(s) 713, 774
SsiI CCGC 3 cut(s) 55, 328, 2519
SspI AATATT 1 cut(s) 2035
SspMI CTAG 5 cut(s) 6, 183, 561, 1110, 1790
StuI AGGCCT 2 cut(s) 713, 774
StyD4I CCNGG 4 cut(s) 291, 2023, 2298, 2413
StyI CCWWGG 3 cut(s) 97, 658, 1003
TaaI ACNGT 4 cut(s) 849, 1808, 2108, 2375
TaiI ACGT 1 cut(s) 1583
TaqI TCGA 5 cut(s) 259, 807, 853, 1705, 2235
TatI WGTACW 2 cut(s) 1811, 2143
TauI GCSGC 1 cut(s) 331
TscAI CASTG 5 cut(s) 132, 852, 1824, 2569, 2575
TseFI GTSAC 1 cut(s) 1654
TseI GCWGC 1 cut(s) 1113
Tsp45I GTSAC 1 cut(s) 1654
TspGWI ACGGA 3 cut(s) 535, 2138, 2432
TspRI CASTG 5 cut(s) 132, 852, 1824, 2569, 2575
Van91I CCANNNNNTGG 2 cut(s) 664, 2164
VneI GTGCAC 1 cut(s) 365
VpaK11BI GGWCC 1 cut(s) 760
VspI ATTAAT 1 cut(s) 1100
XapI RAATTY 9 cut(s) 102, 494, 721, 868, 979, 1631, 1843, 2186, 2291
XbaI TCTAGA 1 cut(s) 182
XcmI CCANNNNNNNNNTGG 2 cut(s) 1001, 2060
XmiI GTMKAC 2 cut(s) 551, 1786
XmnI GAANNNNTTC 4 cut(s) 589, 649, 1943, 2007
XspI CTAG 5 cut(s) 6, 183, 561, 1110, 1790
Zsp2I ATGCAT 2 cut(s) 266, 1840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.