Rw1G004490

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000105
Physical Location & Seq
Reverse (-)
8603219 .. 8607335
4117 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004490.1

Sequence Viewer

Length: 3483 bp
ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTCTTTCTGAGTTTCAGGGGGGCAGATACCCGCGATAATATTACCGGTCATCTGTATCATGCACTAAATCGGAAAGGAATTCTCACCTTTATGGATGACCGAGAGCTTGAGAAAGGAAAATACATTAGGCCTGAACTTTTAGCTGCAATTGAGGAGTCTAGATGTGCGATTGTGATTCTCTCATGGAGATATGCTCGTTCGTCATGGTGCTTGGATGAACTTGTCAAGATTATTCAATGCATGAAAGAGCTGGGCCAACGAGTCCTGCCCGTCTTCTACGGCGTGAATCCTTCTGAAGTGCGGCACCAAAGGGGAAGTTTTGAGCTCAAAAGGAAACCCCAAGTAGAAGTGAAAGTAAGGGAACATGAAGAAGTTTATGGGAAGAATGTGGACAGACTAAATGCCTGGACAGCTGCTTTGACAGAGGTCGCCAATCTTTCTGGCTGGGATTCCAAGAATTATCCATTCGTAAGAGAACTAATTGAGGAAATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAAGGGCTTCATAGGAATGGATTCCCGCATTGATGATCTATTAAGCAATTACATATGTCCACAGCTTTGTGGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAACAGTTGCCCGAGCTATCTACGATGAAATTTGCCAGGATTTTGACCGAAGCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGCTGGCAAAAATTGAAAATATAGACGATGAATACACAGGAGCTGCTATGATAGAAAGGCGGTTGTGTAGAATAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGGCACAATTAGAGAAATTGGCTGGAAGTCACAATTGTTTTGGTCCAGGGAGTAGAATTATCATAACCACCACAGATATTCAGTTGCTAAAGGCACATGATGTTGATGCTACATACGAGGCTAACGGGTTAAACTCTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATGTCCCCCACCAGATGATTATTTGCATCTGTGCTACCATATTTTAGGGTATGCTCAGGGGCTTCCATTGGCTCTTGTGGTTTTAGGTTCTTTTCTATTTGGTAGAAGCACTGATGAATGGGTAAGTGCAATCGATAGGCTAAAGAGCACGCCACATAAACGAATTATTGAGGTGCTTCGGATTAGTTTTGATGGACTGGAAGAAAAAGACAAAGAAATCTTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGTGTGACACAAATACTGGACTATTGCGAGCTAAACCCTGTCATTGGTTTAAGTTTCCTAGCTGATAGATCTCTCATAACTATCTCCAATAACGAACTGTCGATGCATGATTTGCTACAAGAAACAGGCTGGGAAATTGTTCGTGAACAGTCTCCTAAAGAGGCAGGCAAACGTAGTAGATTATGGTCTCATGAAGACATCAACAATGTGCTTAAGAGAAATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACCAAATTACAAGTGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTTCATATTTGTAATGTGGACCTTCCCGAAGGCCTCACTTGTCTTTCTAATTCCTTGAGACTCCTCGAATGGACTGGGTATCCCTTAAGATCTCTCCCACAAAATTTTGAAGCAGATGAGCTCATTGAACTTAACTTTTGCCACAGCAACATTGAACAGCTTTGGAAGGGAACAAAGAATTTTGACAAGCTGAAGTTCATCAAACTTTGCCGTTCTCAAAACATTGTGGAGACCCCAGACCTCGCAGGCGTTCAGAATCTTGAGAGCTTAGATCTTGAAGGATGTGAGAATTTGGTAAGAATCCATCAATCCCTTGCATTGCTCAAAAAGCTTATCTTCGTGAATCTTAAAGACTGCAAAAGCCTCGAAAGTCTGCCAAGTATAATTGAAATGGAATCTCTTGAAACATTAGTTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTTGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTGGCCTTGTGTCATTGAATCTAAGCAACTGCAGAAATCTTGTTTGTCTTCCAAGCACCATCAATAAATTGAAGTCTATTGAAGATCTTAATCTTTCTGGATGCTTGAACCTCGGCAAACCTCAGGTAAATGTGGGGGTGATGGGCTGTTTTGAGGAAACTGATGTGAATAGTGGGTCTGCAATAGAAATGTCATCTACCCATGATCGCATAAAATATGTGGGAAGCTCAATCTTGCCTTCTGGATGGGTGCAAAAAGTGAATACAGAGCCAACGAGTTTTCGCTTGCCTATATCTGGTCTGTGTAATTTAACATATTTGAACCTGAGTAATTGCAATCTTGGTGACGGAGCATTTGCCAATGAATTTGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGCCTTCCTTCAGGCATTGGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATGGTATACTTGATTTAAGGGCAGATGGTTGTACTTCACTGAAGTACTTGTTTAATGCATCAAATTTGAACAGACTAAACAAATCATATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAATAACGTAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAACAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCTATCGGACTGATGGGCGTTCCTTAGGTGTATCTCTACCTGCACATTGGAATAACAGGCGATTTTTGGGATTTGCTTTGTGTGCTGTTTTTGTACTCCATAAGCACCATCGGGGTAAGCTTTCTATTGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTCGATGGAAGAGAATTGGAAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTAAGTCAGATCACCTGTGGCTATTCTATTTATCTCGTGATAAATACTTTGGTACTGAGTGGTGGCATAACAGTTGCAGACGGCTTGAGTTCTTATTTAAAATCAGAGGGCCGGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAAGATGTGCAAGAGCTGAACCAAACGACCACTCAATCAAGCAGTAGGATGTCTCCTTATGAGGATATATTGAATGGTTTTGACTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1160

Amino Acids

131.88

Weight (kDa)

6.42

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 11 - 191 3.4e-50 TIR domain
TIR_2 PF13676 14 - 110 7.4e-15 TIR domain
NB-ARC PF00931 216 - 371 1.6e-18 NB-ARC domain
WHD_ROQ1 PF23282 438 - 509 6.1e-22 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 698 - 782 2.4e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 709 - 777 9.6e-09 Leucine-rich repeat region
C-JID PF20160 987 - 1135 1.9e-19 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 3037
AccB1I GGYRCC 1 cut(s) 342
AccB7I CCANNNNNTGG 2 cut(s) 1621, 2747
AccI GTMKAC 2 cut(s) 560, 2752
AccII CGCG 1 cut(s) 72
AciI CCGC 5 cut(s) 70, 340, 604, 679, 886
AclI AACGTT 1 cut(s) 2167
AclWI GGATC 4 cut(s) 661, 674, 936, 1380
AcuI CTGAAG 7 cut(s) 354, 1910, 2646, 2807, 2924, 3185, 3377
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 2779, 2792, 3084, 3292
AfiI CCNNNNNNNGG 4 cut(s) 1418, 1621, 2528, 2747
AflII CTTAAG 2 cut(s) 1586, 1783
AgeI ACCGGT 1 cut(s) 83
AjnI CCWGG 5 cut(s) 443, 729, 979, 2962, 3234
AjuI GAANNNNNNNTTGG 2 cut(s) 2684, 2716
Alw21I GWGCWC 3 cut(s) 366, 1262, 1821
Alw26I GTCTC 8 cut(s) 1530, 1566, 1689, 1750, 1922, 2186, 2710, 3450
AlwI GGATC 4 cut(s) 661, 674, 936, 1380
AlwNI CAGNNNCTG 1 cut(s) 869
Ama87I CYCGRG 2 cut(s) 705, 2144
AoxI GGCC 6 cut(s) 167, 292, 787, 1729, 2236, 3347
ApeKI GCWGC 4 cut(s) 182, 452, 747, 869
ArsI GACNNNNNNTTYG 4 cut(s) 439, 471, 2570, 2602
AsiGI ACCGGT 1 cut(s) 83
Asp700I GAANNNNTTC 7 cut(s) 598, 810, 1080, 1512, 1606, 2909, 2946
AspS9I GGNCC 4 cut(s) 292, 977, 1717, 3347
AsuC2I CCSGG 1 cut(s) 3351
AsuHPI GGTGA 6 cut(s) 115, 2383, 2588, 2632, 3242, 3373
AvaI CYCGRG 2 cut(s) 705, 2144
AvaII GGWCC 2 cut(s) 977, 1717
AxyI CCTNAGG 2 cut(s) 2355, 3013
BamHI GGATCC 1 cut(s) 666
BanI GGYRCC 1 cut(s) 342
BanII GRGCYC 2 cut(s) 366, 1821
BauI CACGAG 1 cut(s) 3273
BbsI GAAGAC 5 cut(s) 304, 1575, 1688, 2273, 2912
Bbv12I GWGCWC 3 cut(s) 366, 1262, 1821
BbvI GCAGC 4 cut(s) 169, 439, 734, 856
BceAI ACGGC 4 cut(s) 334, 1893, 2243, 3335
BcgI CGANNNNNNTGC 2 cut(s) 2044, 2078
BciT130I CCWGG 5 cut(s) 445, 731, 981, 2964, 3236
BciVI GTATCC 1 cut(s) 1788
BcnI CCSGG 1 cut(s) 3351
BcoDI GTCTC 8 cut(s) 1530, 1566, 1689, 1750, 1922, 2186, 2710, 3450
BfaI CTAG 3 cut(s) 198, 791, 1433
BfmI CTRYAG 1 cut(s) 2263
BfrI CTTAAG 2 cut(s) 1586, 1783
BfuAI ACCTGC 1 cut(s) 3037
BfuI GTATCC 1 cut(s) 1788
BglII AGATCT 4 cut(s) 1442, 1787, 1969, 2317
BisI GCNGC 5 cut(s) 183, 341, 453, 748, 870
BlsI GCNGC 5 cut(s) 184, 342, 454, 749, 871
BmcAI AGTACT 1 cut(s) 2792
Bme1390I CCNGG 6 cut(s) 445, 731, 981, 2964, 3236, 3351
Bme18I GGWCC 2 cut(s) 977, 1717
BmeT110I CYCGRG 2 cut(s) 705, 2144
BmgT120I GGNCC 4 cut(s) 292, 977, 1717, 3347
BmiI GGNNCC 2 cut(s) 344, 668
BmrFI CCNGG 6 cut(s) 445, 731, 981, 2964, 3236, 3351
BmrI ACTGGG 1 cut(s) 1782
BmsI GCATC 6 cut(s) 1030, 1147, 1467, 2324, 2813, 2867
BmuI ACTGGG 1 cut(s) 1782
BoxI GACNNNNGTC 1 cut(s) 464
BpiI GAAGAC 5 cut(s) 304, 1575, 1688, 2273, 2912
BplI GAGNNNNNCTC 2 cut(s) 217, 249
Bpu10I CCTNAGC 1 cut(s) 1167
BpuEI CTTGAG 5 cut(s) 167, 1774, 1979, 2708, 3344
BpuMI CCSGG 1 cut(s) 3351
Bsa29I ATCGAT 1 cut(s) 1245
BsaBI GATNNNNATC 3 cut(s) 927, 1371, 2322
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 1566, 1922
BsaJI CCNNGG 4 cut(s) 670, 980, 1615, 2344
BsaWI WCCGGW 1 cut(s) 83
Bsc4I CCNNNNNNNGG 4 cut(s) 1418, 1621, 2528, 2747
Bse118I RCCGGY 1 cut(s) 83
Bse1I ACTGG 4 cut(s) 1314, 1395, 1777, 2239
Bse21I CCTNAGG 2 cut(s) 2355, 3013
Bse3DI GCAATG 1 cut(s) 2015
Bse8I GATNNNNATC 3 cut(s) 927, 1371, 2322
BseBI CCWGG 5 cut(s) 445, 731, 981, 2964, 3236
BseCI ATCGAT 1 cut(s) 1245
BseDI CCNNGG 4 cut(s) 670, 980, 1615, 2344
BseGI GGATG 7 cut(s) 139, 259, 1985, 2339, 2483, 2882, 3447
BseJI GATNNNNATC 3 cut(s) 927, 1371, 2322
BseLI CCNNNNNNNGG 4 cut(s) 1418, 1621, 2528, 2747
BseMI GCAATG 1 cut(s) 2015
BseMII CTCAG 6 cut(s) 38, 1181, 2369, 2549, 2970, 3285
BseNI ACTGG 4 cut(s) 1314, 1395, 1777, 2239
BseRI GAGGAG 2 cut(s) 206, 1751
BseXI GCAGC 4 cut(s) 169, 439, 734, 856
BseYI CCCAGC 3 cut(s) 289, 483, 1503
BsgI GTGCAG 1 cut(s) 3015
Bsh1236I CGCG 1 cut(s) 72
BshFI GGCC 6 cut(s) 169, 294, 789, 1731, 2238, 3349
BshNI GGYRCC 1 cut(s) 342
BshTI ACCGGT 1 cut(s) 83
BshVI ATCGAT 1 cut(s) 1245
BsiHKAI GWGCWC 3 cut(s) 366, 1262, 1821
BsiHKCI CYCGRG 2 cut(s) 705, 2144
BsiSI CCGG 2 cut(s) 84, 3350
BslFI GGGAC 1 cut(s) 1101
BslI CCNNNNNNNGG 4 cut(s) 1418, 1621, 2528, 2747
BsmAI GTCTC 8 cut(s) 1530, 1566, 1689, 1750, 1922, 2186, 2710, 3450
BsmFI GGGAC 1 cut(s) 1101
BsnI GGCC 6 cut(s) 169, 294, 789, 1731, 2238, 3349
Bso31I GGTCTC 2 cut(s) 1566, 1922
BsoBI CYCGRG 2 cut(s) 705, 2144
Bsp1286I GDGCHC 3 cut(s) 366, 1262, 1821
Bsp19I CCATGG 1 cut(s) 670
BspACI CCGC 5 cut(s) 70, 340, 604, 679, 886
BspANI GGCC 6 cut(s) 169, 294, 789, 1731, 2238, 3349
BspCNI CTCAG 6 cut(s) 39, 1180, 2368, 2550, 2971, 3286
BspDI ATCGAT 1 cut(s) 1245
BspFNI CGCG 1 cut(s) 72
BspHI TCATGA 1 cut(s) 1564
BspLI GGNNCC 2 cut(s) 344, 668
BspMAI CTGCAG 1 cut(s) 2267
BspMI ACCTGC 1 cut(s) 3037
BspPI GGATC 4 cut(s) 661, 674, 936, 1380
BspT107I GGYRCC 1 cut(s) 342
BspTI CTTAAG 2 cut(s) 1586, 1783
BspTNI GGTCTC 2 cut(s) 1566, 1922
BsrDI GCAATG 1 cut(s) 2015
BsrFI RCCGGY 1 cut(s) 83
BsrI ACTGG 4 cut(s) 1314, 1395, 1777, 2239
BssAI RCCGGY 1 cut(s) 83
BssECI CCNNGG 4 cut(s) 670, 980, 1615, 2344
BssNAI GTATAC 2 cut(s) 561, 2753
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 3273
BssT1I CCWWGG 2 cut(s) 670, 1615
Bst1107I GTATAC 2 cut(s) 561, 2753
Bst2BI CACGAG 1 cut(s) 3273
Bst2UI CCWGG 5 cut(s) 445, 731, 981, 2964, 3236
Bst4CI ACNGT 4 cut(s) 700, 1473, 1524, 3311
Bst6I CTCTTC 2 cut(s) 19, 3172
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 2 cut(s) 1586, 1783
BstAPI GCANNNNNTGC 2 cut(s) 1486, 3400
BstC8I GCNNGC 9 cut(s) 677, 831, 1262, 1348, 1403, 1540, 1945, 2519, 3207
BstDSI CCRYGG 1 cut(s) 670
BstEII GGTNACC 1 cut(s) 2620
BstF5I GGATG 7 cut(s) 139, 259, 1985, 2339, 2483, 2882, 3447
BstFNI CGCG 1 cut(s) 72
BstMAI GTCTC 8 cut(s) 1530, 1566, 1689, 1750, 1922, 2186, 2710, 3450
BstMWI GCNNNNNNNGC 7 cut(s) 449, 681, 1486, 2026, 2683, 3071, 3400
BstNI CCWGG 5 cut(s) 445, 731, 981, 2964, 3236
BstNSI RCATGY 1 cut(s) 679
BstPAI GACNNNNGTC 1 cut(s) 464
BstPI GGTNACC 1 cut(s) 2620
BstSCI CCNGG 6 cut(s) 443, 729, 979, 2962, 3234, 3349
BstSFI CTRYAG 1 cut(s) 2263
BstUI CGCG 1 cut(s) 72
BstV1I GCAGC 4 cut(s) 169, 439, 734, 856
BstV2I GAAGAC 5 cut(s) 304, 1575, 1688, 2273, 2912
BstX2I RGATCY 5 cut(s) 666, 1442, 1787, 1969, 2317
BstYI RGATCY 5 cut(s) 666, 1442, 1787, 1969, 2317
BstZ17I GTATAC 2 cut(s) 561, 2753
Bsu15I ATCGAT 1 cut(s) 1245
Bsu36I CCTNAGG 2 cut(s) 2355, 3013
BsuI GTATCC 1 cut(s) 1788
BsuRI GGCC 6 cut(s) 169, 294, 789, 1731, 2238, 3349
BsuTUI ATCGAT 1 cut(s) 1245
BtgI CCRYGG 1 cut(s) 670
BtsCI GGATG 7 cut(s) 139, 259, 1985, 2339, 2483, 2882, 3447
BtsIMutI CAGTG 2 cut(s) 1221, 2783
BveI ACCTGC 1 cut(s) 3037
Cac8I GCNNGC 9 cut(s) 677, 831, 1262, 1348, 1403, 1540, 1945, 2519, 3207
CaiI CAGNNNCTG 1 cut(s) 869
CciI TCATGA 1 cut(s) 1564
Cfr10I RCCGGY 1 cut(s) 83
Cfr13I GGNCC 4 cut(s) 292, 977, 1717, 3347
ClaI ATCGAT 1 cut(s) 1245
Csp6I GTAC 4 cut(s) 2778, 2791, 3083, 3291
CspAI ACCGGT 1 cut(s) 83
CviQI GTAC 4 cut(s) 2778, 2791, 3083, 3291
DraI TTTAAA 1 cut(s) 3337
Eam1104I CTCTTC 2 cut(s) 19, 3172
EarI CTCTTC 2 cut(s) 19, 3172
Ecl136II GAGCTC 2 cut(s) 364, 1819
Eco130I CCWWGG 2 cut(s) 670, 1615
Eco147I AGGCCT 2 cut(s) 169, 1731
Eco24I GRGCYC 2 cut(s) 366, 1821
Eco31I GGTCTC 2 cut(s) 1566, 1922
Eco47I GGWCC 2 cut(s) 977, 1717
Eco53kI GAGCTC 2 cut(s) 364, 1819
Eco57I CTGAAG 7 cut(s) 354, 1910, 2646, 2807, 2924, 3185, 3377
Eco81I CCTNAGG 2 cut(s) 2355, 3013
Eco88I CYCGRG 2 cut(s) 705, 2144
Eco91I GGTNACC 1 cut(s) 2620
EcoICRI GAGCTC 2 cut(s) 364, 1819
EcoO65I GGTNACC 1 cut(s) 2620
EcoRI GAATTC 2 cut(s) 117, 3122
EcoRII CCWGG 5 cut(s) 443, 729, 979, 2962, 3234
EcoT14I CCWWGG 2 cut(s) 670, 1615
EcoT22I ATGCAT 3 cut(s) 281, 1482, 2806
EcoT38I GRGCYC 2 cut(s) 366, 1821
ErhI CCWWGG 2 cut(s) 670, 1615
FalI AAGNNNNNCTT 4 cut(s) 795, 827, 2018, 2050
FaqI GGGAC 1 cut(s) 1101
FauI CCCGC 3 cut(s) 77, 611, 672
FauNDI CATATG 1 cut(s) 632
FblI GTMKAC 2 cut(s) 560, 2752
Fnu4HI GCNGC 5 cut(s) 183, 341, 453, 748, 870
FokI GGATG 7 cut(s) 146, 266, 1992, 2346, 2490, 2889, 3454
FriOI GRGCYC 2 cut(s) 366, 1821
Fsp4HI GCNGC 5 cut(s) 183, 341, 453, 748, 870
FspBI CTAG 3 cut(s) 198, 791, 1433
GluI GCNGC 5 cut(s) 183, 341, 453, 748, 870
GsaI CCCAGC 3 cut(s) 293, 487, 1507
HaeIII GGCC 6 cut(s) 169, 294, 789, 1731, 2238, 3349
HapII CCGG 2 cut(s) 84, 3350
Hin1I GRCGYC 1 cut(s) 5
HincII GTYRAC 3 cut(s) 9, 577, 1635
HindII GTYRAC 3 cut(s) 9, 577, 1635
HindIII AAGCTT 3 cut(s) 2027, 2684, 3107
HpaII CCGG 2 cut(s) 84, 3350
HphI GGTGA 6 cut(s) 115, 2383, 2588, 2632, 3242, 3373
Hpy166II GTNNAC 9 cut(s) 9, 430, 561, 577, 638, 1520, 1635, 1717, 2753
Hpy8I GTNNAC 9 cut(s) 9, 430, 561, 577, 638, 1520, 1635, 1717, 2753
Hpy99I CGWCG 1 cut(s) 24
HpyCH4III ACNGT 4 cut(s) 700, 1473, 1524, 3311
HpyCH4IV ACGT 3 cut(s) 1546, 2167, 2886
HpyF10VI GCNNNNNNNGC 7 cut(s) 449, 681, 1486, 2026, 2683, 3071, 3400
HpySE526I ACGT 3 cut(s) 1546, 2167, 2886
Hsp92I GRCGYC 1 cut(s) 5
LmnI GCTCC 2 cut(s) 866, 2582
Lsp1109I GCAGC 4 cut(s) 169, 439, 734, 856
LweI GCATC 6 cut(s) 1030, 1147, 1467, 2324, 2813, 2867
MaeI CTAG 3 cut(s) 198, 791, 1433
MaeII ACGT 3 cut(s) 1546, 2167, 2886
MaeIII GTNAC 5 cut(s) 962, 1378, 2576, 2603, 2620
MfeI CAATTG 4 cut(s) 186, 967, 2220, 2848
MflI RGATCY 5 cut(s) 666, 1442, 1787, 1969, 2317
MhlI GDGCHC 3 cut(s) 366, 1262, 1821
MlyI GAGTC 4 cut(s) 203, 309, 1752, 3384
MmeI TCCRAC 1 cut(s) 2134
Mph1103I ATGCAT 3 cut(s) 281, 1482, 2806
MroXI GAANNNNTTC 7 cut(s) 598, 810, 1080, 1512, 1606, 2909, 2946
MslI CAYNNNNRTG 3 cut(s) 128, 593, 1625
MspA1I CMGCKG 1 cut(s) 452
MspCI CTTAAG 2 cut(s) 1586, 1783
MspI CCGG 2 cut(s) 84, 3350
MspR9I CCNGG 6 cut(s) 445, 731, 981, 2964, 3236, 3351
MunI CAATTG 4 cut(s) 186, 967, 2220, 2848
MvaI CCWGG 5 cut(s) 445, 731, 981, 2964, 3236
MvnI CGCG 1 cut(s) 72
MwoI GCNNNNNNNGC 7 cut(s) 449, 681, 1486, 2026, 2683, 3071, 3400
NciI CCSGG 1 cut(s) 3351
NcoI CCATGG 1 cut(s) 670
NdeI CATATG 1 cut(s) 632
NlaIV GGNNCC 2 cut(s) 344, 668
NmeAIII GCCGAG 1 cut(s) 2325
NmuCI GTSAC 4 cut(s) 962, 1378, 2576, 2620
NsiI ATGCAT 3 cut(s) 281, 1482, 2806
NspI RCATGY 1 cut(s) 679
PaeI GCATGC 1 cut(s) 679
PagI TCATGA 1 cut(s) 1564
PceI AGGCCT 2 cut(s) 169, 1731
PdmI GAANNNNTTC 7 cut(s) 598, 810, 1080, 1512, 1606, 2909, 2946
PflMI CCANNNNNTGG 2 cut(s) 1621, 2747
PfoI TCCNGGA 1 cut(s) 2962
PinAI ACCGGT 1 cut(s) 83
PkrI GCNGC 5 cut(s) 184, 342, 454, 749, 871
PleI GAGTC 4 cut(s) 202, 308, 1752, 3383
PpsI GAGTC 4 cut(s) 202, 308, 1752, 3383
PshAI GACNNNNGTC 1 cut(s) 464
Psp124BI GAGCTC 2 cut(s) 366, 1821
Psp1406I AACGTT 1 cut(s) 2167
Psp6I CCWGG 5 cut(s) 443, 729, 979, 2962, 3234
PspEI GGTNACC 1 cut(s) 2620
PspFI CCCAGC 3 cut(s) 289, 483, 1503
PspGI CCWGG 5 cut(s) 443, 729, 979, 2962, 3234
PspN4I GGNNCC 2 cut(s) 344, 668
PspPI GGNCC 4 cut(s) 292, 977, 1717, 3347
PstI CTGCAG 1 cut(s) 2267
PstNI CAGNNNCTG 1 cut(s) 869
PsuI RGATCY 5 cut(s) 666, 1442, 1787, 1969, 2317
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 4 cut(s) 2779, 2792, 3084, 3292
RsaNI GTAC 4 cut(s) 2778, 2791, 3083, 3291
RseI CAYNNNNRTG 3 cut(s) 128, 593, 1625
SacI GAGCTC 2 cut(s) 366, 1821
SatI GCNGC 5 cut(s) 183, 341, 453, 748, 870
Sau96I GGNCC 4 cut(s) 292, 977, 1717, 3347
ScaI AGTACT 1 cut(s) 2792
SchI GAGTC 4 cut(s) 203, 309, 1752, 3384
ScrFI CCNGG 6 cut(s) 445, 731, 981, 2964, 3236, 3351
SduI GDGCHC 3 cut(s) 366, 1262, 1821
SfaNI GCATC 6 cut(s) 1030, 1147, 1467, 2324, 2813, 2867
SfcI CTRYAG 1 cut(s) 2263
SinI GGWCC 2 cut(s) 977, 1717
SmiMI CAYNNNNRTG 3 cut(s) 128, 593, 1625
SmlI CTYRAG 7 cut(s) 146, 1586, 1753, 1783, 1958, 2687, 3323
SmoI CTYRAG 7 cut(s) 146, 1586, 1753, 1783, 1958, 2687, 3323
SphI GCATGC 1 cut(s) 679
SseBI AGGCCT 2 cut(s) 169, 1731
SsiI CCGC 5 cut(s) 70, 340, 604, 679, 886
SspI AATATT 2 cut(s) 79, 2974
SspMI CTAG 3 cut(s) 198, 791, 1433
SstI GAGCTC 2 cut(s) 366, 1821
StuI AGGCCT 2 cut(s) 169, 1731
StyD4I CCNGG 6 cut(s) 443, 729, 979, 2962, 3234, 3349
StyI CCWWGG 2 cut(s) 670, 1615
TaaI ACNGT 4 cut(s) 700, 1473, 1524, 3311
TaiI ACGT 3 cut(s) 1549, 2170, 2889
TaqI TCGA 5 cut(s) 1245, 1475, 1764, 2064, 3171
TaqII GACCGA 2 cut(s) 153, 756
TatI WGTACW 3 cut(s) 2777, 2790, 3082
TauI GCSGC 1 cut(s) 343
TscAI CASTG 2 cut(s) 1228, 2790
TseFI GTSAC 4 cut(s) 962, 1378, 2576, 2620
TseI GCWGC 4 cut(s) 182, 452, 747, 869
Tsp45I GTSAC 4 cut(s) 962, 1378, 2576, 2620
TspGWI ACGGA 3 cut(s) 544, 2595, 3368
TspRI CASTG 2 cut(s) 1228, 2790
Van91I CCANNNNNTGG 2 cut(s) 1621, 2747
Vha464I CTTAAG 2 cut(s) 1586, 1783
VpaK11BI GGWCC 2 cut(s) 977, 1717
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 1 cut(s) 679
XcmI CCANNNNNNNNNTGG 2 cut(s) 826, 1645
XmiI GTMKAC 2 cut(s) 560, 2752
XmnI GAANNNNTTC 7 cut(s) 598, 810, 1080, 1512, 1606, 2909, 2946
XspI CTAG 3 cut(s) 198, 791, 1433
ZrmI AGTACT 1 cut(s) 2792
Zsp2I ATGCAT 3 cut(s) 281, 1482, 2806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.