Rmu_sc0012920.1_g000010

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0012920.1
OG0000105
Physical Location & Seq
Reverse (-)
37816 .. 46807
8992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0012920.1_g000010.1.cds

Sequence Viewer

Length: 4485 bp
ctgtatcgcctcaaaaagccttcaaacaaagctccagaacccgacggggctgcacaagaaactgccgctcattcttcttgccgtcgccctggtctgaacaccgcacagtcgccgccgccacacgaaaaccctagttttcgccttctcgagatcactccggaaagaacggaggccctgcttttgagatcgatggcgtcaacctcttctcgtcgtcaatggaaatacgatgtctttctaagtttcaggggggaagatacgcgctggtcttttaccgagtatctgtatattgtactaaatcagagaggaattctcacctttcgggatgaccgagagcttgagaaaggaaaatccattaggcccgaacttttaggtgcaattaaggagtctagatctgcgattgtcattctctcacccagatatggtcgttcgtcatggtgcttggatgaactcgtcaagattattcaatgcatgaaagagatgggccaacaagtcttccccatcttctacggcgtgaatccttctgatgtgcggcaccaaacgggatgttttgagctcaaatggatatcccaagtagatgtgcaagtaagggaacatgaagaagtttatgggaagtataatgaggacagaataaatgcctggagagctgctttggctgaggtcgccaatctttctggctgggaattcaactattatggaagtgtaacaaaactaattgaggaaattgctaatcatatgctaaagatgcccgtgcagacaccttcaagtgttgacaagggcttcataggaatgggttcccgcattgatgatttattaagcaattacatatatccgcagcttgatgcggtgcgttttatagggatccatggcatgcggggcataggtaagacaacacttgctcgagctatccatgatgaaatttgtcaggattttgaccgagcctactttctttccaatgttagagaaaggtctaaaaacaatggcctagtttctcttcaagaaaaacttctttctgaaatcctaatggcaaaaattaaaaatatagcggatgaatacacaggagctgctatgataaaaaggcggttgtgtagaataaaggtgcttgttgttattgatgatgtggatcacttgacacaattagagaaattggctggaagtcgcaactggtttggttctgggagtagaattgtcataaccaccacagatgttcagttgttaaaggcacatgctgttgatgctacatacaaggctaacgggttaaactgtgatgaagcacttcaacttttgagtttgaaggcttttaagaaatatcccccaccagcagattatttgcatctgtgctacgatattatagggtatgcccaggggcttccgttggctctcgtggttttaggttcttttctatttggtagaagaactgatgaatgggcaagtgcgatagataggctaaagaacacaccagataaacgcattattgatgtgcttcggattagttttgatggactggatgaaaaagacaaagaaatattcctacatattgcttgcttttgcaaggggaaggataaggatcgtgtgacacaaatactagactattgccagctaaaccctgtcattggtttaagtgttcttgctgatagatctctcataactctctccaacaacgaactgtggatgcatgatttgctgcaagaattgggctgggaaattgttcgtgaacagtctcccaaagagccaggcaaacgtagtagattatggtctcatgacgacatcaacaatgtgctgaagaaaaataggggaacggattcaatccaaggcatggtaatcgagttgactgaattacaagtggctcattggaatccagaagccttttcaaatttgtctcagcttggtcttctccatattcgtaatgtggaccttcccaaaggcctcaattgtctttctaattccttgagactcctcgaatggactgggtatcccttaagatctctcccaaaattttttgaaggagatgaacttattgaacttaacttgtgccacagcaacattaaacagctttggaagggaacaaagaattttgacaagttgaagttcatcaaactctgccattctcaaagcattgtggagaccccagacctcgcaggcgttcagaatcttgagagtttagatcttgaagggtgtgagaatttgagtctgccaagtagaattgaaatggaatctcttgaaacattaattctttctaactgctcaaaagttaagaagattcctgagtttgttggaaatatggaacgtttgtcggtgctttatcttgatgagactgccattgaggaactgccagtatcaattgaacggctgactggccttgtgacattgaatctgagcaactgcagaaatcttgtctgtcttcctagcaccgtcgatatattgaagtctgttgaaaatcttaatctttctggatgcttgaaactcggcaaacatcaggtaaacgtgggggtgatggactgttttgaggaaactgatgtgaatagtgggtccgcaatagaaatgtctcctacccatgatcgcagaaaatatgtgagaggttcaatctttcatggatgcaaagtagtttggcgatctttaaaaaaggtcttgccttctggattggtgcaaaaagtgaatacagagccaaagagtttccactttcctatagcacaaaaagtgaacacagagccaatgagtttccgcttgcctatatctggtctgtgtaatttaacatatctgaacctgagtaattgcaatcttggtgaaggagcatttgccaacgaatttggttactttcccactttggtgaccttgaatctaagtgggaacaattttgttcgtcttccttcaggcattggattactttctaggcttgagaactttaacttggagaattgcaagagacttcaagagttgtcagaccttccatcaaatagtatactagatttaagggcagatggttgtacttcactgaaatacttgtttgatgcatcaaatttggacaaattaaccaaatcatatttcaatttcatcaattgcttcaatctaaatggcaatcaaggatgcaataacatagcatttgaaatgctgaagacattaatgtatcagggaatctctaatgacagggaaacttttcaaattgtaattcctggaagtaatattcctgagtggttcagccatcagagtgttgggtgttctgtaagtgtatctctgcctgcacattggaataatagtcgggttttgggatttgctctgtgtgctgtttttgtactccatgagcaccatcgggtggatgagctttatatagatgaattcaagacttttaatgcaacacatcatcttgtatgttgcctcaagctcgatggaagagaattggaagtatatggcagacagcctgcatttcgctttagtgaagaattttgcaaggttaagtcagatcacctgtggctattctatgtatctcatgataaatactttggtacagagcggtggcataacagttgcagtcagtttgagttcttatttgaaaccagagggccaggtctgatggtgaaggagtgcggagtccgtctgatatatgagcaagatgtgcaagagttgaaccgaacaaccacttcatcaagcagtgggatgtctccttatggggatatattgattggttttgacattccagttgcaggggaaaccagtggcagtgcgacactgttagctagggatagatctgtagctgggttgatattcattctggcatcagataaatcttgcaagcacgtaaccatcgatcctacatctgaggcggagacaaaacttatgtccctggttaaaatggggtacagtgaattggaggtttcaatggcaatagaaagatgcagtattgcttcatcagttgttgatttgactgatttcatatcagctgctcaaatttcaaaggcagaagatggctatcttccccttgaagagatggtattgtttgtccttcttgctgatattcaatattttgactgctggtacaacttttacagcgtacacgtgtggagtgagtatcctgagaagagtaagaggaaactttgtgaatcttcgttgctgaaaaggaaaaggattatgggacatggaaaccagatcataggggaagatgctgatgcaattcatctcccaaatccaatgattgggtttgggactccagctgatctctgtcaaatctaccaaaatcttccaaaggcagccgcaggacctccttatttttactatgagaatgtggcattgacacgtaaaggagtttggagcacaatatcccgctttttgtatgacatgaaacctgagtttgttgttttgaagtatttatgtgctgctgcaagaaaaaggggccatgtttacaatcttccaatcaaaaacagattccgtctcgttccccttccaccacaaactatatttgatgacaaggaggtggtggcggcctacttgggatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1494

Amino Acids

169.14

Weight (kDa)

6.46

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 531
AccB7I CCANNNNNTGG 3 cut(s) 1813, 3307, 4184
AccBSI CCGCTC 2 cut(s) 68, 3505
AccI GTMKAC 1 cut(s) 2956
AccII CGCG 1 cut(s) 259
AccIII TCCGGA 1 cut(s) 157
AclI AACGTT 1 cut(s) 2284
AclWI GGATC 5 cut(s) 853, 866, 1128, 1572, 3794
AcuI CTGAAG 3 cut(s) 1799, 2850, 3128
AcvI CACGTG 1 cut(s) 4048
AcyI GRCGYC 1 cut(s) 194
AfaI GTAC 7 cut(s) 291, 2983, 3288, 3499, 3851, 4028, 4044
AfiI CCNNNNNNNGG 7 cut(s) 419, 1610, 1813, 2732, 3307, 3695, 4184
AflII CTTAAG 1 cut(s) 1975
AflIII ACRYGT 3 cut(s) 4045, 4047, 4283
AjnI CCWGG 7 cut(s) 88, 635, 1359, 1729, 3166, 3556, 3834
AjuI GAANNNNNNNTTGG 4 cut(s) 2888, 2920, 3023, 3055
AleI CACNNNNGTG 1 cut(s) 2822
Alw21I GWGCWC 3 cut(s) 555, 3300, 4304
AlwI GGATC 5 cut(s) 853, 866, 1128, 1572, 3794
AlwNI CAGNNNCTG 1 cut(s) 1061
Ama87I CYCGRG 2 cut(s) 146, 897
Aor13HI TCCGGA 1 cut(s) 157
AoxI GGCC 9 cut(s) 171, 356, 481, 979, 1921, 2353, 3554, 4381, 4470
ApeKI GCWGC 9 cut(s) 50, 644, 832, 1061, 1681, 3932, 4238, 4364, 4367
ArsI GACNNNNNNTTYG 2 cut(s) 3815, 3847
AseI ATTAAT 2 cut(s) 2225, 3116
Asp700I GAANNNNTTC 6 cut(s) 790, 1002, 1272, 1704, 1798, 3150
AspLEI GCGC 1 cut(s) 261
AspS9I GGNCC 8 cut(s) 172, 357, 481, 1909, 2526, 3554, 4247, 4381
AsuHPI GGTGA 7 cut(s) 304, 402, 2500, 2792, 2836, 3449, 3580
AvaI CYCGRG 2 cut(s) 146, 897
AvaII GGWCC 3 cut(s) 1909, 2526, 4247
BamHI GGATCC 1 cut(s) 858
BanI GGYRCC 1 cut(s) 531
BanII GRGCYC 1 cut(s) 555
BauI CACGAG 1 cut(s) 1379
BbrPI CACGTG 1 cut(s) 4048
BbsI GAAGAC 5 cut(s) 484, 1880, 2390, 2852, 3116
Bbv12I GWGCWC 3 cut(s) 555, 3300, 4304
BbvCI CCTCAGC 1 cut(s) 654
BbvI GCAGC 9 cut(s) 37, 631, 844, 1048, 1668, 3919, 4250, 4351, 4354
BceAI ACGGC 3 cut(s) 66, 523, 2360
BcgI CGANNNNNNTGC 4 cut(s) 32, 66, 1801, 1835
BciT130I CCWGG 7 cut(s) 90, 637, 1361, 1731, 3168, 3558, 3836
BciVI GTATCC 2 cut(s) 1980, 4071
BfaI CTAG 8 cut(s) 132, 387, 983, 1583, 2403, 2886, 2960, 3729
BfmI CTRYAG 3 cut(s) 2380, 2682, 3741
BfrI CTTAAG 1 cut(s) 1975
BfuI GTATCC 2 cut(s) 1980, 4071
BglII AGATCT 5 cut(s) 389, 1634, 1979, 2161, 3737
Bme1390I CCNGG 7 cut(s) 90, 637, 1361, 1731, 3168, 3558, 3836
Bme18I GGWCC 3 cut(s) 1909, 2526, 4247
BmeT110I CYCGRG 2 cut(s) 146, 897
BmgT120I GGNCC 8 cut(s) 172, 357, 481, 1909, 2526, 3554, 4247, 4381
BmiI GGNNCC 5 cut(s) 533, 793, 860, 2527, 4382
BmrFI CCNGG 7 cut(s) 90, 637, 1361, 1731, 3168, 3558, 3836
BmrI ACTGGG 1 cut(s) 1974
BmuI ACTGGG 1 cut(s) 1974
BpiI GAAGAC 5 cut(s) 484, 1880, 2390, 2852, 3116
BplI GAGNNNNNCTC 2 cut(s) 294, 326
BpmI CTGGAG 3 cut(s) 18, 658, 4182
Bpu10I CCTNAGC 1 cut(s) 654
BpuEI CTTGAG 5 cut(s) 356, 1966, 2171, 2912, 3356
Bsa29I ATCGAT 2 cut(s) 188, 3798
BsaAI YACGTR 3 cut(s) 3790, 4048, 4286
BsaBI GATNNNNATC 3 cut(s) 1119, 1563, 3960
BsaHI GRCGYC 1 cut(s) 194
BsaI GGTCTC 2 cut(s) 1758, 2114
BsaJI CCNNGG 6 cut(s) 88, 862, 1359, 1360, 1807, 3834
BsaWI WCCGGW 1 cut(s) 157
Bsc4I CCNNNNNNNGG 7 cut(s) 419, 1610, 1813, 2732, 3307, 3695, 4184
Bse1I ACTGG 7 cut(s) 1166, 1506, 1969, 2330, 2356, 3689, 3705
Bse8I GATNNNNATC 3 cut(s) 1119, 1563, 3960
BseAI TCCGGA 1 cut(s) 157
BseBI CCWGG 7 cut(s) 90, 637, 1361, 1731, 3168, 3558, 3836
BseCI ATCGAT 2 cut(s) 188, 3798
BseDI CCNNGG 6 cut(s) 88, 862, 1359, 1360, 1807, 3834
BseJI GATNNNNATC 3 cut(s) 1119, 1563, 3960
BseLI CCNNNNNNNGG 7 cut(s) 419, 1610, 1813, 2732, 3307, 3695, 4184
BseMII CTCAG 9 cut(s) 645, 1892, 2253, 2363, 2753, 3174, 3801, 4056, 4326
BseNI ACTGG 7 cut(s) 1166, 1506, 1969, 2330, 2356, 3689, 3705
BseRI GAGGAG 1 cut(s) 1943
BseXI GCAGC 9 cut(s) 37, 631, 844, 1048, 1668, 3919, 4250, 4351, 4354
BseYI CCCAGC 3 cut(s) 675, 1695, 3746
BsgI GTGCAG 3 cut(s) 36, 770, 3219
Bsh1236I CGCG 1 cut(s) 259
BshFI GGCC 9 cut(s) 173, 358, 483, 981, 1923, 2355, 3556, 4383, 4472
BshNI GGYRCC 1 cut(s) 531
BshVI ATCGAT 2 cut(s) 188, 3798
BsiHKAI GWGCWC 3 cut(s) 555, 3300, 4304
BsiHKCI CYCGRG 2 cut(s) 146, 897
BsiSI CCGG 1 cut(s) 158
BslFI GGGAC 3 cut(s) 3817, 4137, 4207
BslI CCNNNNNNNGG 7 cut(s) 419, 1610, 1813, 2732, 3307, 3695, 4184
BsmBI CGTCTC 1 cut(s) 4424
BsmFI GGGAC 3 cut(s) 3817, 4137, 4207
BsnI GGCC 9 cut(s) 173, 358, 483, 981, 1923, 2355, 3556, 4383, 4472
Bso31I GGTCTC 2 cut(s) 1758, 2114
BsoBI CYCGRG 2 cut(s) 146, 897
Bsp1286I GDGCHC 3 cut(s) 555, 3300, 4304
Bsp13I TCCGGA 1 cut(s) 157
Bsp19I CCATGG 1 cut(s) 862
BspANI GGCC 9 cut(s) 173, 358, 483, 981, 1923, 2355, 3556, 4383, 4472
BspCNI CTCAG 9 cut(s) 646, 1891, 2254, 2364, 2754, 3175, 3802, 4057, 4327
BspDI ATCGAT 2 cut(s) 188, 3798
BspEI TCCGGA 1 cut(s) 157
BspFNI CGCG 1 cut(s) 259
BspHI TCATGA 2 cut(s) 1756, 3481
BspLI GGNNCC 5 cut(s) 533, 793, 860, 2527, 4382
BspMAI CTGCAG 1 cut(s) 2384
BspPI GGATC 5 cut(s) 853, 866, 1128, 1572, 3794
BspT107I GGYRCC 1 cut(s) 531
BspTI CTTAAG 1 cut(s) 1975
BspTNI GGTCTC 2 cut(s) 1758, 2114
BsrBI CCGCTC 2 cut(s) 68, 3505
BsrI ACTGG 7 cut(s) 1166, 1506, 1969, 2330, 2356, 3689, 3705
BssECI CCNNGG 6 cut(s) 88, 862, 1359, 1360, 1807, 3834
BssNAI GTATAC 1 cut(s) 2957
BssNI GRCGYC 1 cut(s) 194
BssSI CACGAG 1 cut(s) 1379
BssT1I CCWWGG 2 cut(s) 862, 1807
Bst1107I GTATAC 1 cut(s) 2957
Bst2BI CACGAG 1 cut(s) 1379
Bst2UI CCWGG 7 cut(s) 90, 637, 1361, 1731, 3168, 3558, 3836
Bst4CI ACNGT 9 cut(s) 108, 1262, 1665, 1716, 2410, 2498, 3518, 3723, 3854
Bst6I CTCTTC 5 cut(s) 208, 996, 3379, 3969, 4064
BstACI GRCGYC 1 cut(s) 194
BstAFI CTTAAG 1 cut(s) 1975
BstAPI GCANNNNNTGC 2 cut(s) 1678, 3607
BstBAI YACGTR 3 cut(s) 3790, 4048, 4286
BstC8I GCNNGC 8 cut(s) 869, 1540, 1595, 2137, 2723, 3234, 3414, 3785
BstDSI CCRYGG 1 cut(s) 862
BstEII GGTNACC 1 cut(s) 2824
BstFNI CGCG 1 cut(s) 259
BstHHI GCGC 1 cut(s) 261
BstMWI GCNNNNNNNGC 9 cut(s) 641, 650, 659, 742, 873, 1232, 1678, 3275, 3607
BstNI CCWGG 7 cut(s) 90, 637, 1361, 1731, 3168, 3558, 3836
BstNSI RCATGY 2 cut(s) 871, 1226
BstPI GGTNACC 1 cut(s) 2824
BstSCI CCNGG 7 cut(s) 88, 635, 1359, 1729, 3166, 3556, 3834
BstSFI CTRYAG 3 cut(s) 2380, 2682, 3741
BstUI CGCG 1 cut(s) 259
BstV1I GCAGC 9 cut(s) 37, 631, 844, 1048, 1668, 3919, 4250, 4351, 4354
BstV2I GAAGAC 5 cut(s) 484, 1880, 2390, 2852, 3116
BstX2I RGATCY 6 cut(s) 389, 858, 1634, 1979, 2161, 3737
BstYI RGATCY 6 cut(s) 389, 858, 1634, 1979, 2161, 3737
BstZ17I GTATAC 1 cut(s) 2957
Bsu15I ATCGAT 2 cut(s) 188, 3798
BsuI GTATCC 2 cut(s) 1980, 4071
BsuRI GGCC 9 cut(s) 173, 358, 483, 981, 1923, 2355, 3556, 4383, 4472
BsuTUI ATCGAT 2 cut(s) 188, 3798
BtgI CCRYGG 1 cut(s) 862
BtsI GCAGTG 2 cut(s) 3649, 3718
BtsIMutI CAGTG 6 cut(s) 2987, 3649, 3712, 3718, 3719, 3859
Cac8I GCNNGC 8 cut(s) 869, 1540, 1595, 2137, 2723, 3234, 3414, 3785
CaiI CAGNNNCTG 1 cut(s) 1061
CciI TCATGA 2 cut(s) 1756, 3481
CfoI GCGC 1 cut(s) 261
Cfr13I GGNCC 8 cut(s) 172, 357, 481, 1909, 2526, 3554, 4247, 4381
ClaI ATCGAT 2 cut(s) 188, 3798
CseI GACGC 1 cut(s) 183
Csp6I GTAC 7 cut(s) 290, 2982, 3287, 3498, 3850, 4027, 4043
CviQI GTAC 7 cut(s) 290, 2982, 3287, 3498, 3850, 4027, 4043
DraI TTTAAA 1 cut(s) 2616
Eam1104I CTCTTC 5 cut(s) 208, 996, 3379, 3969, 4064
EarI CTCTTC 5 cut(s) 208, 996, 3379, 3969, 4064
EciI GGCGGA 1 cut(s) 3830
Ecl136II GAGCTC 1 cut(s) 553
Eco130I CCWWGG 2 cut(s) 862, 1807
Eco147I AGGCCT 1 cut(s) 1923
Eco24I GRGCYC 1 cut(s) 555
Eco31I GGTCTC 2 cut(s) 1758, 2114
Eco32I GATATC 1 cut(s) 564
Eco47I GGWCC 3 cut(s) 1909, 2526, 4247
Eco53kI GAGCTC 1 cut(s) 553
Eco57I CTGAAG 3 cut(s) 1799, 2850, 3128
Eco72I CACGTG 1 cut(s) 4048
Eco88I CYCGRG 2 cut(s) 146, 897
Eco91I GGTNACC 1 cut(s) 2824
EcoICRI GAGCTC 1 cut(s) 553
EcoO109I RGGNCCY 2 cut(s) 172, 4247
EcoO65I GGTNACC 1 cut(s) 2824
EcoRI GAATTC 3 cut(s) 306, 680, 3329
EcoRII CCWGG 7 cut(s) 88, 635, 1359, 1729, 3166, 3556, 3834
EcoRV GATATC 1 cut(s) 564
EcoT14I CCWWGG 2 cut(s) 862, 1807
EcoT22I ATGCAT 3 cut(s) 470, 1674, 3010
EcoT38I GRGCYC 1 cut(s) 555
ErhI CCWWGG 2 cut(s) 862, 1807
Esp3I CGTCTC 1 cut(s) 4424
FalI AAGNNNNNCTT 2 cut(s) 987, 1019
FaqI GGGAC 3 cut(s) 3817, 4137, 4207
FauI CCCGC 3 cut(s) 803, 864, 4319
FauNDI CATATG 1 cut(s) 732
FblI GTMKAC 1 cut(s) 2956
FriOI GRGCYC 1 cut(s) 555
FspBI CTAG 8 cut(s) 132, 387, 983, 1583, 2403, 2886, 2960, 3729
GlaI GCGC 1 cut(s) 260
GsaI CCCAGC 3 cut(s) 679, 1699, 3750
GsuI CTGGAG 3 cut(s) 18, 658, 4182
HaeIII GGCC 9 cut(s) 173, 358, 483, 981, 1923, 2355, 3556, 4383, 4472
HapII CCGG 1 cut(s) 158
HgaI GACGC 1 cut(s) 183
HhaI GCGC 1 cut(s) 261
Hin1I GRCGYC 1 cut(s) 194
Hin6I GCGC 1 cut(s) 259
HinP1I GCGC 1 cut(s) 259
HincII GTYRAC 3 cut(s) 198, 769, 1827
HindII GTYRAC 3 cut(s) 198, 769, 1827
HpaII CCGG 1 cut(s) 158
HphI GGTGA 7 cut(s) 304, 402, 2500, 2792, 2836, 3449, 3580
Hpy99I CGWCG 4 cut(s) 47, 87, 213, 2414
HpyCH4III ACNGT 9 cut(s) 108, 1262, 1665, 1716, 2410, 2498, 3518, 3723, 3854
HpyCH4IV ACGT 6 cut(s) 1738, 2284, 2481, 3789, 4047, 4285
HpyF10VI GCNNNNNNNGC 9 cut(s) 641, 650, 659, 742, 873, 1232, 1678, 3275, 3607
HpySE526I ACGT 6 cut(s) 1738, 2284, 2481, 3789, 4047, 4285
Hsp92I GRCGYC 1 cut(s) 194
HspAI GCGC 1 cut(s) 259
Kpn2I TCCGGA 1 cut(s) 157
LmnI GCTCC 4 cut(s) 37, 1058, 2786, 4299
Lsp1109I GCAGC 9 cut(s) 37, 631, 844, 1048, 1668, 3919, 4250, 4351, 4354
MaeI CTAG 8 cut(s) 132, 387, 983, 1583, 2403, 2886, 2960, 3729
MaeII ACGT 6 cut(s) 1738, 2284, 2481, 3789, 4047, 4285
MaeIII GTNAC 6 cut(s) 700, 1570, 2359, 2807, 2824, 3790
MbiI CCGCTC 2 cut(s) 68, 3505
MfeI CAATTG 3 cut(s) 1927, 2337, 3052
MflI RGATCY 6 cut(s) 389, 858, 1634, 1979, 2161, 3737
MhlI GDGCHC 3 cut(s) 555, 3300, 4304
MlyI GAGTC 5 cut(s) 392, 1944, 2194, 3591, 4189
MmeI TCCRAC 2 cut(s) 1677, 2251
Mph1103I ATGCAT 3 cut(s) 470, 1674, 3010
MroI TCCGGA 1 cut(s) 157
MroXI GAANNNNTTC 6 cut(s) 790, 1002, 1272, 1704, 1798, 3150
MslI CAYNNNNRTG 3 cut(s) 785, 2822, 3201
MspA1I CMGCKG 2 cut(s) 3932, 4202
MspCI CTTAAG 1 cut(s) 1975
MspI CCGG 1 cut(s) 158
MspR9I CCNGG 7 cut(s) 90, 637, 1361, 1731, 3168, 3558, 3836
MunI CAATTG 3 cut(s) 1927, 2337, 3052
MvaI CCWGG 7 cut(s) 90, 637, 1361, 1731, 3168, 3558, 3836
MvnI CGCG 1 cut(s) 259
MwoI GCNNNNNNNGC 9 cut(s) 641, 650, 659, 742, 873, 1232, 1678, 3275, 3607
NcoI CCATGG 1 cut(s) 862
NdeI CATATG 1 cut(s) 732
NlaIV GGNNCC 5 cut(s) 533, 793, 860, 2527, 4382
NmeAIII GCCGAG 1 cut(s) 2442
NmuCI GTSAC 3 cut(s) 1570, 2359, 2824
NsiI ATGCAT 3 cut(s) 470, 1674, 3010
NspI RCATGY 2 cut(s) 871, 1226
OliI CACNNNNGTG 1 cut(s) 2822
PaeI GCATGC 1 cut(s) 871
PaeR7I CTCGAG 2 cut(s) 146, 897
PagI TCATGA 2 cut(s) 1756, 3481
PasI CCCWGGG 1 cut(s) 1360
PceI AGGCCT 1 cut(s) 1923
PcsI WCGNNNNNNNCGW 2 cut(s) 325, 3795
PdmI GAANNNNTTC 6 cut(s) 790, 1002, 1272, 1704, 1798, 3150
PflMI CCANNNNNTGG 3 cut(s) 1813, 3307, 4184
PfoI TCCNGGA 1 cut(s) 3166
PleI GAGTC 5 cut(s) 391, 1944, 2193, 3590, 4189
PmaCI CACGTG 1 cut(s) 4048
PmlI CACGTG 1 cut(s) 4048
PpsI GAGTC 5 cut(s) 391, 1944, 2193, 3590, 4189
Ppu21I YACGTR 3 cut(s) 3790, 4048, 4286
PpuMI RGGWCCY 1 cut(s) 4247
PshBI ATTAAT 2 cut(s) 2225, 3116
Psp124BI GAGCTC 1 cut(s) 555
Psp1406I AACGTT 1 cut(s) 2284
Psp5II RGGWCCY 1 cut(s) 4247
Psp6I CCWGG 7 cut(s) 88, 635, 1359, 1729, 3166, 3556, 3834
PspCI CACGTG 1 cut(s) 4048
PspEI GGTNACC 1 cut(s) 2824
PspFI CCCAGC 3 cut(s) 675, 1695, 3746
PspGI CCWGG 7 cut(s) 88, 635, 1359, 1729, 3166, 3556, 3834
PspN4I GGNNCC 5 cut(s) 533, 793, 860, 2527, 4382
PspPI GGNCC 8 cut(s) 172, 357, 481, 1909, 2526, 3554, 4247, 4381
PspPPI RGGWCCY 1 cut(s) 4247
PspXI VCTCGAGB 1 cut(s) 897
PstI CTGCAG 1 cut(s) 2384
PstNI CAGNNNCTG 1 cut(s) 1061
PsuI RGATCY 6 cut(s) 389, 858, 1634, 1979, 2161, 3737
PvuII CAGCTG 2 cut(s) 3932, 4202
RsaI GTAC 7 cut(s) 291, 2983, 3288, 3499, 3851, 4028, 4044
RsaNI GTAC 7 cut(s) 290, 2982, 3287, 3498, 3850, 4027, 4043
RseI CAYNNNNRTG 3 cut(s) 785, 2822, 3201
SacI GAGCTC 1 cut(s) 555
Sau96I GGNCC 8 cut(s) 172, 357, 481, 1909, 2526, 3554, 4247, 4381
SchI GAGTC 5 cut(s) 392, 1944, 2194, 3591, 4189
ScrFI CCNGG 7 cut(s) 90, 637, 1361, 1731, 3168, 3558, 3836
SduI GDGCHC 3 cut(s) 555, 3300, 4304
SfcI CTRYAG 3 cut(s) 2380, 2682, 3741
Sfr274I CTCGAG 2 cut(s) 146, 897
SinI GGWCC 3 cut(s) 1909, 2526, 4247
SlaI CTCGAG 2 cut(s) 146, 897
SmiMI CAYNNNNRTG 3 cut(s) 785, 2822, 3201
SmlI CTYRAG 8 cut(s) 146, 335, 897, 1945, 1975, 2150, 2891, 3371
SmoI CTYRAG 8 cut(s) 146, 335, 897, 1945, 1975, 2150, 2891, 3371
SphI GCATGC 1 cut(s) 871
SseBI AGGCCT 1 cut(s) 1923
SspI AATATT 3 cut(s) 1524, 3178, 4013
SspMI CTAG 8 cut(s) 132, 387, 983, 1583, 2403, 2886, 2960, 3729
SstI GAGCTC 1 cut(s) 555
StuI AGGCCT 1 cut(s) 1923
StyD4I CCNGG 7 cut(s) 88, 635, 1359, 1729, 3166, 3556, 3834
StyI CCWWGG 2 cut(s) 862, 1807
TaaI ACNGT 9 cut(s) 108, 1262, 1665, 1716, 2410, 2498, 3518, 3723, 3854
TaiI ACGT 6 cut(s) 1741, 2287, 2484, 3792, 4050, 4288
TaqI TCGA 8 cut(s) 147, 188, 898, 1821, 1956, 2412, 3378, 3798
TaqII GACCGA 2 cut(s) 342, 948
TatI WGTACW 3 cut(s) 289, 2981, 3286
TauI GCSGC 6 cut(s) 68, 115, 118, 532, 4244, 4472
TscAI CASTG 6 cut(s) 2994, 3649, 3712, 3718, 3726, 3859
TseFI GTSAC 3 cut(s) 1570, 2359, 2824
TseI GCWGC 9 cut(s) 50, 644, 832, 1061, 1681, 3932, 4238, 4364, 4367
Tsp45I GTSAC 3 cut(s) 1570, 2359, 2824
TspGWI ACGGA 5 cut(s) 182, 1359, 1811, 3575, 4406
TspRI CASTG 6 cut(s) 2994, 3649, 3712, 3718, 3726, 3859
Van91I CCANNNNNTGG 3 cut(s) 1813, 3307, 4184
Vha464I CTTAAG 1 cut(s) 1975
VpaK11BI GGWCC 3 cut(s) 1909, 2526, 4247
VspI ATTAAT 2 cut(s) 2225, 3116
XbaI TCTAGA 1 cut(s) 386
XceI RCATGY 2 cut(s) 871, 1226
XcmI CCANNNNNNNNNTGG 1 cut(s) 3203
XhoI CTCGAG 2 cut(s) 146, 897
XmiI GTMKAC 1 cut(s) 2956
XmnI GAANNNNTTC 6 cut(s) 790, 1002, 1272, 1704, 1798, 3150
XspI CTAG 8 cut(s) 132, 387, 983, 1583, 2403, 2886, 2960, 3729
Zsp2I ATGCAT 3 cut(s) 470, 1674, 3010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.