Rh6DG032400

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000105
Physical Location & Seq
Forward (+)
3184750 .. 3189542
4793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG032400.1

Sequence Viewer

Length: 444 bp
ATGCCTTCTGATGTGCTGCGCCTTCTCAAAAGGAAAACCGGACTCGGCCTTCTCAAAAAGAGAGTATATATGAACCGGACCCGGCTCATATATGTTGATTATGAGCAACATGTAGAATTAAGGTTACACGAAGACGTTTATGGCAAGAAGATTTTGTCCTCAGAGGCGTTGAGCGCTGCTTTGACAGAGTTAGCCTATCTAGAAGGGTGGGGTTCAAAGAATTTTAGACAGCCTGCATTTCGCTTTAGTGAAGAGTTTTGCCAATTGGACTCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTGGTGGCATAACAGTTGCAGTCGGCTTGATTTCTTATTTGAAACTAGAGGGCCAGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATATGAGCAAGATGTGCAAGAGTTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR UreF

Protein Analysis

147

Amino Acids

17.48

Weight (kDa)

7.72

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 220
AcuI CTGAAG 1 cut(s) 404
AfaI GTAC 1 cut(s) 319
AfeI AGCGCT 1 cut(s) 175
AflIII ACRYGT 1 cut(s) 109
AgsI TTSAA 3 cut(s) 216, 365, 439
AjnI CCWGG 1 cut(s) 376
Aor51HI AGCGCT 1 cut(s) 175
AoxI GGCC 2 cut(s) 46, 374
ApeKI GCWGC 2 cut(s) 16, 176
ApoI RAATTY 1 cut(s) 220
AspLEI GCGC 2 cut(s) 21, 176
AspS9I GGNCC 2 cut(s) 78, 374
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 2 cut(s) 269, 400
AvaII GGWCC 1 cut(s) 78
BauI CACGAG 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 138
BbvI GCAGC 2 cut(s) 3, 163
BciT130I CCWGG 1 cut(s) 378
BcnI CCSGG 1 cut(s) 82
BfaI CTAG 2 cut(s) 200, 369
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 2 cut(s) 17, 177
BlsI GCNGC 2 cut(s) 18, 178
Bme1390I CCNGG 2 cut(s) 82, 378
Bme18I GGWCC 1 cut(s) 78
BmgT120I GGNCC 2 cut(s) 78, 374
BmiI GGNNCC 1 cut(s) 80
BmrFI CCNGG 2 cut(s) 82, 378
BpiI GAAGAC 1 cut(s) 138
BpuMI CCSGG 1 cut(s) 82
BsaWI WCCGGW 2 cut(s) 38, 75
BseBI CCWGG 1 cut(s) 378
BseMII CTCAG 2 cut(s) 174, 285
BseXI GCAGC 2 cut(s) 3, 163
BshFI GGCC 2 cut(s) 48, 376
BsiSI CCGG 3 cut(s) 39, 76, 82
BsnI GGCC 2 cut(s) 48, 376
Bsp143I GATC 1 cut(s) 274
BspANI GGCC 2 cut(s) 48, 376
BspCNI CTCAG 2 cut(s) 173, 284
BspLI GGNNCC 1 cut(s) 80
BssMI GATC 1 cut(s) 274
BssSI CACGAG 1 cut(s) 300
Bst2BI CACGAG 1 cut(s) 300
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 1 cut(s) 338
Bst6I CTCTTC 1 cut(s) 246
BstAPI GCANNNNNTGC 1 cut(s) 427
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 2 cut(s) 160, 271
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 2 cut(s) 21, 176
BstKTI GATC 1 cut(s) 277
BstMBI GATC 1 cut(s) 274
BstMWI GCNNNNNNNGC 2 cut(s) 173, 427
BstNI CCWGG 1 cut(s) 378
BstNSI RCATGY 1 cut(s) 113
BstSCI CCNGG 2 cut(s) 80, 376
BstV1I GCAGC 2 cut(s) 3, 163
BstV2I GAAGAC 1 cut(s) 138
BsuRI GGCC 2 cut(s) 48, 376
Cac8I GCNNGC 1 cut(s) 234
CfoI GCGC 2 cut(s) 21, 176
Cfr13I GGNCC 2 cut(s) 78, 374
Csp6I GTAC 1 cut(s) 318
CviAII CATG 1 cut(s) 110
CviJI RGCY 7 cut(s) 48, 85, 194, 232, 286, 349, 376
CviKI_1 RGCY 7 cut(s) 48, 85, 194, 232, 286, 349, 376
CviQI GTAC 1 cut(s) 318
DdeI CTNAG 2 cut(s) 160, 271
DpnI GATC 1 cut(s) 276
DpnII GATC 1 cut(s) 274
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 78
Eco47III AGCGCT 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 404
EcoRII CCWGG 1 cut(s) 376
FaeI CATG 1 cut(s) 113
FatI CATG 1 cut(s) 109
Fnu4HI GCNGC 2 cut(s) 17, 177
Fsp4HI GCNGC 2 cut(s) 17, 177
FspBI CTAG 2 cut(s) 200, 369
GlaI GCGC 2 cut(s) 20, 175
GluI GCNGC 2 cut(s) 17, 177
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 2 cut(s) 48, 376
HapII CCGG 3 cut(s) 39, 76, 82
HhaI GCGC 2 cut(s) 21, 176
Hin1II CATG 1 cut(s) 113
Hin6I GCGC 2 cut(s) 19, 174
HinP1I GCGC 2 cut(s) 19, 174
HinfI GANTC 3 cut(s) 42, 269, 402
HpaII CCGG 3 cut(s) 39, 76, 82
HphI GGTGA 2 cut(s) 269, 400
Hpy188I TCNGA 5 cut(s) 10, 163, 274, 384, 411
Hpy188III TCNNGA 2 cut(s) 200, 302
HpyAV CCTTC 6 cut(s) 15, 32, 59, 197, 379, 385
HpyCH4III ACNGT 1 cut(s) 338
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 3 cut(s) 236, 342, 430
HpyF10VI GCNNNNNNNGC 2 cut(s) 173, 427
HpyF3I CTNAG 2 cut(s) 160, 271
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 1 cut(s) 113
HspAI GCGC 2 cut(s) 19, 174
Kzo9I GATC 1 cut(s) 274
LpnPI CCDG 7 cut(s) 52, 89, 95, 246, 293, 363, 390
Lsp1109I GCAGC 2 cut(s) 3, 163
MaeI CTAG 2 cut(s) 200, 369
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 1 cut(s) 123
MalI GATC 1 cut(s) 276
MboI GATC 1 cut(s) 274
MboII GAAGA 3 cut(s) 143, 160, 263
MfeI CAATTG 1 cut(s) 263
MluCI AATT 3 cut(s) 116, 220, 263
MlyI GAGTC 3 cut(s) 36, 263, 411
MnlI CCTC 3 cut(s) 157, 169, 365
MseI TTAA 1 cut(s) 119
MspI CCGG 3 cut(s) 39, 76, 82
MspR9I CCNGG 2 cut(s) 82, 378
MunI CAATTG 1 cut(s) 263
MvaI CCWGG 1 cut(s) 378
MwoI GCNNNNNNNGC 2 cut(s) 173, 427
NciI CCSGG 1 cut(s) 82
NdeII GATC 1 cut(s) 274
NlaIII CATG 1 cut(s) 113
NlaIV GGNNCC 1 cut(s) 80
NmeAIII GCCGAG 1 cut(s) 24
NspI RCATGY 1 cut(s) 113
PciI ACATGT 1 cut(s) 109
PkrI GCNGC 2 cut(s) 18, 178
PleI GAGTC 3 cut(s) 36, 263, 410
PpsI GAGTC 3 cut(s) 36, 263, 410
PscI ACATGT 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 376
PspN4I GGNNCC 1 cut(s) 80
PspPI GGNCC 2 cut(s) 78, 374
RsaI GTAC 1 cut(s) 319
RsaNI GTAC 1 cut(s) 318
SaqAI TTAA 1 cut(s) 119
SatI GCNGC 2 cut(s) 17, 177
Sau3AI GATC 1 cut(s) 274
Sau96I GGNCC 2 cut(s) 78, 374
SchI GAGTC 3 cut(s) 36, 263, 411
ScrFI CCNGG 2 cut(s) 82, 378
SetI ASST 5 cut(s) 125, 138, 282, 382, 390
SinI GGWCC 1 cut(s) 78
Sse9I AATT 3 cut(s) 116, 220, 263
SspMI CTAG 2 cut(s) 200, 369
StyD4I CCNGG 2 cut(s) 80, 376
TaaI ACNGT 1 cut(s) 338
TaiI ACGT 1 cut(s) 138
TasI AATT 3 cut(s) 116, 220, 263
Tru1I TTAA 1 cut(s) 119
Tru9I TTAA 1 cut(s) 119
TseI GCWGC 2 cut(s) 16, 176
TspDTI ATGAA 1 cut(s) 86
TspGWI ACGGA 1 cut(s) 395
VpaK11BI GGWCC 1 cut(s) 78
XapI RAATTY 1 cut(s) 220
XbaI TCTAGA 1 cut(s) 199
XceI RCATGY 1 cut(s) 113
XspI CTAG 2 cut(s) 200, 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.