Rorug01G0036100

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000105
Physical Location & Seq
Reverse (-)
6058871 .. 6062965
4095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0036100.1

Sequence Viewer

Length: 4095 bp
ATGGCAGTGGGAGAGATCTTTCTTGCGGCATTCCTTCAGTTGCTGCTGGACAGGTTAACGCCTCGGGAGATCCTGGGCTACTTTGGAAGCTTGGGAGGAGCTGGCAAAAAGCTGCAGAAATGGAGAGAGACACTTTCCGCAGTTGCAGCCGTGCTTTACGATGCGGAGGAGAAGCAGCTGACGAACAAGGCAGTGGATTTGTGGCTGAATGACCTCAAACACTTGGCCTCTGATATGGACGATCTACTCGACACCTTTTCCACTGAGATGTTAGCACGCAAGCAGCTCAAGCTTCACCAAACTGGCACAAGCAAGGTACGAGGCTTCTTTACCAAAGTTCCTCATAAAGTCAAGTTCAATTTCAATATGAGCTCCGAAATAGACGAGATTGCTAACCGCTTGCAAGACATACTTGACCGGAAAGAGATACTCGGTTTGAAATATATTGAGCATACCTCTTCAATAGCATCATCATCACAAAGGACACCAAGTTCGTATGTGCTAGATGGACCTGTGGTTGGAAGAGATGAAGACACAAGAAAGATTGTTGAATTGTTGTCCAGCGATGTTGATCCTTCTTCTACCAATAAATATCAAGTTGTTGCCATTGTTGGTATGGGAGGACTCGGCAAGACAACGCTTGCGGGACAAGTCTTCAATGATGTAGCTGCAATGGAACAGTTTGATCTCAAGGTCTGGGTTTCAGTGTCTGATGACTTCGATCTTCAAACGGTGACGAGAGCTATTTTTAAGGAAGTTACATCTCGGCCTTGTGATATGGATGAGTTCAGTCGACTTCAAGATAATCTGAGCAAGGAGATCGATGGTAAAAGGTTTTTGATTGTTTTAGATGATGTCTGGAGCACGTGTGGTTATGATTCATGGACAAAACTGCAAGCTCCCTTTCGTAGGGGAGCTAAGGGAAGTAAGGTAATGGTGACAACACGTGATGAAAAAGTTGCTGCATTTATGGGAGCCCCAGCCGCTGCAGTTTATTATTTAAAGACTCTATCAGATGAAGGTTGTTTGCAAGTATTTGAGCAGCATGTTAGCAATGATAGGCCACCGAATTTCGAGTTGCTTAAGAAGAAAATTGTTACGAACTGCAATGGATTGCCATTGGCTGCAAAAACTCTTGGTGGTGTTTTACGTTGTAAAGAAACTGACAAATGGGAGGAAATGCTGAATGACAAGTTATGGAGTATATCAGATGAGAGTAATATACTCCCAGTACTCAGGTTGAGTTATCATTATCTCCCTTCACCTTTGAAGAGGTGCTTTGCCTATTGCTCAATACTTCCCAATGACTATGAATTTGGGAAAACCCAATTAATCCTTCTGTGGATGGCAGAGGGTTTCCTTGAGCAAACAGAAGGGACTAAAGGAATGGAAGATATCGGTGACAAATATTTTGGGGAGCTATTATCTCGGTCATTATTTCAAACCTCGGGCAAAAACAGTTCATGCTATGTAATGCATGACCTTGTTGGTGATTTGGCACGATGGGCTGCTGCAGACACATTTTGCAGATTGGAGGATAAACAGCATGGTAGATGTTCTGCAAAGACTCGTCATCTGAGTTACATTTCTGCTAAGTTTGATGGGGAAAAAAGATTTGAGACACTTTCTGGAGCAAAACGCTTGCGGACTTTCCTACCACTTCCTGTTTCCGGTGGTTATGAGAATTATCTAACTCGTTATGCTACTTTTGATATATTGCCACAATTGAAGTACTTGCGAGTGCTCTCCTTCAATGGCTATAAACTGACCGAGGTGCCAAATTCCGTAGGTAAGTTGAGACATCTACGGTACCTTGATCTTTCTCACACATTAATAACGTGTTTGCTTGAGTCAACATGCATGCTTTACAACTTACAGACGTTAATATTAGAGAATTTTTCAAAATTGAAGACACTACCTTCAAACATGAGCAATTTATCTAATTTACGTCATCTCAACAATTCAAACATTCCTTCATTGGAAGAAATGCCTCCCAAAGTAAGTCGGTTGACTCATCTGCGAACTTTGCCTAATTTTGTGGTGGGGAAAGGTAGTGCATCAGGGATTGGAGAGATACAGTCATTGCATCTTCGTGGGACCTTGAGTGTTAGAAGATTGGAAAATGTGATTGATGTTGGGGATGCGAAGACGGCTCAAATAATGAACAAGGAAGGGCTGGAAATCTTGCAATTGGAATGGAGCGGCACAAGAGAGAAGGAGTTGGAGGTGCTGTGCAGTTTAGAACCTCATAAAAAGCTTAAGGAACTGACCATCAAGGGCTACAATGGTTTCGAATTTTCAAAATGGATTGGACATCCTTCATTCTCTGATATGACGTCTGTGAAATTGGAGAATTGTAAAAACTGTCGATTCTTACCTCCTCTTGGCCAATTACCTTTTCTGAAAAAACTTCAGATACAAGGACTGGCCAATGTTAAAAGTGTGGGTGCTGAGTTTTATGGAGAGTGCAGCTTGCCTTTTCCAGTGTTGGAAGACCTGAGGTTCGAGGATATGCAAAATTGGAAGGAGTGGCTTCCTTGCAAAAGAGATGAAGAAATTCGAGTTTTCCCATGCATGGAAGAGCTTTTAATCTTTAGTTGCCCCAAATTGAAAGGTTGGTTGCCCAAGAACATGGATTCATTATCACAGATGTATATTGCTGAATGTGAAGAGTTAGTGGTTTCAATAGCCAATTATAAACATCTTCGTATGCTGATCATCAGCGGTTGTAAAAGGGTGGTGCACAGAAGTGGAGTTAAGTTTGAGTTACTGGAGATTATGGGGCTTCATAATATTTCAGAGTTCAGTCTCGAAATAGACGGGTTCATCAGAGGATTGCCAAAGCTTCAAGGATTGTGGATTACTGGTTGTGAAGAGTTGACATGTTTATGGGAGAATGAGGATAGGTGGCTGCAGCGTCGAATTTCCAATATTATTGGAGGCAAGATATCTGACTCCCCCTCTCACTTTATTCAAGAGCTAAGAGCACTCAGTGTACTTGAGTTAACTGGATGCTCAAATCTAATTTCTTTTCCAGAAGCTAGTTGGCCACCTTGCCTTGAATTTGTAAAGATGGTGTCGTGTAATTCTTTGACGCATTTTGTAAGGTATCAAGTACCGCCAAGCATAAGAAGAATAGAGATAGAAAAATGTCAAAATTTGAAACTATTAGTGAAGGATGCTGGGGAGGTAGGGGGTTGTCTGGAGTACTTGGAGATAACAGCGTGTGGATCTTTGACATCCTTAACAGGCAAAGGAGGCCGACTACCCAGAACGCTCAAACATCTTTATTTAAGTGACTGTGAACAGTTGGAGTCAATAATCAAAACATTGCACGATGACACTTGTCTTGAAGATCTTAGGATACAGAGGTGTGCAAATTTCAAATCCTTACCAGAGGGGCTATGCCATCTCTCCGCTCTTCGCATGTTATGGATAGAGGAATGTGGAAGTCTCGTTTCCTTCCCAAGAGAAGGGTGGCCATCCAACCTGGCATGCTTGTCTATCTCCATTTGTGCTCAATTGGAAGCCCTCCCCAGAGGCATGGACAACCTCAGCTCTCTTCAGATTTTGTTGTTGAGATACTGTGGAGGTTTGGCATCCATTCTAGAAGAGGGTTTCCCACCCAACCTAATTAATCTGGAAATTGGCCATCCCGAAAGCTGCAATCCTCTCTCACAGTGGGGGCTTCGCCACTTGGACAGACTCACCTCTCTTGAAACCTTTTCTATCTACAGTGTAGATCCAGATCTGGTGTCCTTTCCGCCAAAGGAGGTGCTGCTCCCTAAATCTCTCATTAAACTCACCATTGCAGGCTTCCCAAATCTGCAGCGCCTGTCATCATCTCTTCAATCACTCACCTCTCTTGAATCCCTTGACATTCTTGGATGCCCAAAGCTAGCATCTATTATTCCAGAAAAAGAGGATCATCTGCCTCCTTCACTTACACAGCTTCGCATCCAGGACGGCTGTCCGCTGCTCACAAAGAAATACCAACCAGGTAAAGCACTACACTGGCCCAAACAAATTGCCCACATCCCTTACGTTTATGTTGGAGACTGCGATGACGAAGCCGAAGCCGACCGCCGGGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1364

Amino Acids

153.78

Weight (kDa)

6.35

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 10 - 99 3.5e-18 Rx N-terminal domain
NB-ARC PF00931 195 - 352 3.3e-37 NB-ARC domain
WHD_DRP PF23559 432 - 500 5e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 573 - 838 3.3e-21 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 689 - 808 2.4e-34 R13L1/DRL21 LRRs
LRR_14 PF23598 1092 - 1294 6e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2697
AatII GACGTC 1 cut(s) 2339
Acc65I GGTACC 1 cut(s) 1809
AccB1I GGYRCC 2 cut(s) 1774, 1809
AccBSI CCGCTC 2 cut(s) 2202, 3419
AccI GTMKAC 1 cut(s) 793
AclWI GGATC 5 cut(s) 64, 566, 3238, 3737, 3933
AcoI YGGCCR 5 cut(s) 2386, 2427, 3047, 3479, 3649
AcuI CTGAAG 3 cut(s) 20, 2396, 3548
AcvI CACGTG 2 cut(s) 867, 947
AcyI GRCGYC 1 cut(s) 2336
AdeI CACNNNGTG 1 cut(s) 2993
AfaI GTAC 7 cut(s) 318, 1233, 1733, 1811, 2997, 3117, 3209
AfiI CCNNNNNNNGG 7 cut(s) 518, 909, 1670, 2384, 2575, 3473, 4086
AflII CTTAAG 2 cut(s) 1082, 2258
AflIII ACRYGT 4 cut(s) 866, 944, 1838, 2882
AjnI CCWGG 4 cut(s) 72, 3489, 3960, 3997
AjuI GAANNNNNNNTTGG 4 cut(s) 1320, 1352, 2382, 2414
AleI CACNNNNGTG 1 cut(s) 2748
AloI GAACNNNNNNTCC 2 cut(s) 475, 507
Alw21I GWGCWC 6 cut(s) 374, 866, 1746, 2745, 2989, 3520
Alw26I GTCTC 6 cut(s) 122, 1613, 1792, 2813, 3458, 4050
Alw44I GTGCAC 1 cut(s) 2741
AlwI GGATC 5 cut(s) 64, 566, 3238, 3737, 3933
AlwNI CAGNNNCTG 4 cut(s) 43, 710, 986, 3835
Ama87I CYCGRG 2 cut(s) 63, 1447
ApaLI GTGCAC 1 cut(s) 2741
ArsI GACNNNNNNTTYG 8 cut(s) 475, 507, 1298, 1330, 1394, 1426, 2486, 2518
AseI ATTAAT 3 cut(s) 1331, 1832, 3636
Asp700I GAANNNNTTC 1 cut(s) 2556
Asp718I GGTACC 1 cut(s) 1809
AspLEI GCGC 1 cut(s) 3834
AspS9I GGNCC 3 cut(s) 509, 2097, 4017
AsuC2I CCSGG 1 cut(s) 4088
AsuHPI GGTGA 9 cut(s) 287, 745, 949, 1254, 1412, 1502, 3700, 3796, 3850
AsuII TTCGAA 1 cut(s) 2292
AsuNHI GCTAGC 1 cut(s) 3898
AvaI CYCGRG 2 cut(s) 63, 1447
AvaII GGWCC 2 cut(s) 509, 2097
AxyI CCTNAGG 1 cut(s) 2498
BaeGI GKGCMC 1 cut(s) 2745
BaeI ACNNNNGTAYC 2 cut(s) 3356, 3389
BalI TGGCCA 5 cut(s) 2388, 2429, 3049, 3481, 3651
BanI GGYRCC 2 cut(s) 1774, 1809
BanII GRGCYC 2 cut(s) 374, 979
BbrPI CACGTG 2 cut(s) 867, 947
BbsI GAAGAC 5 cut(s) 537, 646, 1916, 2153, 2499
Bbv12I GWGCWC 6 cut(s) 374, 866, 1746, 2745, 2989, 3520
BbvCI CCTCAGC 1 cut(s) 3554
BceAI ACGGC 3 cut(s) 134, 2166, 3982
BcgI CGANNNNNNTGC 2 cut(s) 802, 836
BciT130I CCWGG 4 cut(s) 74, 3491, 3962, 3999
BciVI GTATCC 1 cut(s) 3357
BclI TGATCA 1 cut(s) 2715
BcnI CCSGG 1 cut(s) 4088
BcoDI GTCTC 6 cut(s) 122, 1613, 1792, 2813, 3458, 4050
BfaI CTAG 4 cut(s) 503, 3042, 3608, 3899
BfmI CTRYAG 6 cut(s) 113, 987, 1512, 2912, 3733, 3827
BfoI RGCGCY 1 cut(s) 3835
BfrI CTTAAG 2 cut(s) 1082, 2258
BfuI GTATCC 1 cut(s) 3357
BglII AGATCT 3 cut(s) 15, 3355, 3748
BmcAI AGTACT 3 cut(s) 1233, 1733, 3209
Bme1390I CCNGG 5 cut(s) 74, 3491, 3962, 3999, 4088
Bme18I GGWCC 2 cut(s) 509, 2097
BmeT110I CYCGRG 2 cut(s) 63, 1447
BmgT120I GGNCC 3 cut(s) 509, 2097, 4017
BmiI GGNNCC 4 cut(s) 976, 1776, 1811, 2098
BmrFI CCNGG 5 cut(s) 74, 3491, 3962, 3999, 4088
BmrI ACTGGG 1 cut(s) 1223
BmtI GCTAGC 1 cut(s) 3902
BmuI ACTGGG 1 cut(s) 1223
BoxI GACNNNNGTC 2 cut(s) 3345, 3969
BpiI GAAGAC 5 cut(s) 537, 646, 1916, 2153, 2499
BplI GAGNNNNNCTC 2 cut(s) 440, 472
BpmI CTGGAG 4 cut(s) 880, 1650, 2792, 3224
Bpu10I CCTNAGC 2 cut(s) 918, 3554
Bpu14I TTCGAA 1 cut(s) 2292
BpuEI CTTGAG 6 cut(s) 272, 674, 1382, 1868, 2122, 3020
BpuMI CCSGG 1 cut(s) 4088
Bsa29I ATCGAT 1 cut(s) 822
BsaAI YACGTR 2 cut(s) 867, 947
BsaBI GATNNNNATC 2 cut(s) 570, 3747
BsaHI GRCGYC 1 cut(s) 2336
BsaJI CCNNGG 4 cut(s) 62, 73, 1446, 1770
BsaWI WCCGGW 2 cut(s) 417, 1670
BsaXI ACNNNNNCTCC 4 cut(s) 853, 883, 2216, 2246
Bsc4I CCNNNNNNNGG 7 cut(s) 518, 909, 1670, 2384, 2575, 3473, 4086
Bse1I ACTGG 8 cut(s) 307, 1229, 2430, 2483, 2775, 2869, 3013, 4019
Bse21I CCTNAGG 1 cut(s) 2498
Bse3DI GCAATG 6 cut(s) 678, 1060, 1114, 2081, 3329, 3807
Bse8I GATNNNNATC 2 cut(s) 570, 3747
BseBI CCWGG 4 cut(s) 74, 3491, 3962, 3999
BseCI ATCGAT 1 cut(s) 822
BseDI CCNNGG 4 cut(s) 62, 73, 1446, 1770
BseJI GATNNNNATC 2 cut(s) 570, 3747
BseLI CCNNNNNNNGG 7 cut(s) 518, 909, 1670, 2384, 2575, 3473, 4086
BseMI GCAATG 6 cut(s) 678, 1060, 1114, 2081, 3329, 3807
BseMII CTCAG 8 cut(s) 255, 800, 1249, 1568, 2442, 2489, 3004, 3568
BseNI ACTGG 8 cut(s) 307, 1229, 2430, 2483, 2775, 2869, 3013, 4019
BseRI GAGGAG 3 cut(s) 111, 182, 2370
BseSI GKGCMC 1 cut(s) 2745
BseYI CCCAGC 2 cut(s) 979, 3182
BsgI GTGCAG 2 cut(s) 2254, 2488
Bsh1285I CGRYCG 1 cut(s) 4084
BshNI GGYRCC 2 cut(s) 1774, 1809
BshVI ATCGAT 1 cut(s) 822
BsiEI CGRYCG 1 cut(s) 4084
BsiHKAI GWGCWC 6 cut(s) 374, 866, 1746, 2745, 2989, 3520
BsiHKCI CYCGRG 2 cut(s) 63, 1447
BsiSI CCGG 3 cut(s) 418, 1671, 4087
BslFI GGGAC 3 cut(s) 660, 1390, 2110
BslI CCNNNNNNNGG 7 cut(s) 518, 909, 1670, 2384, 2575, 3473, 4086
BsmAI GTCTC 6 cut(s) 122, 1613, 1792, 2813, 3458, 4050
BsmFI GGGAC 3 cut(s) 660, 1390, 2110
BsmI GAATGC 1 cut(s) 29
BsoBI CYCGRG 2 cut(s) 63, 1447
Bsp119I TTCGAA 1 cut(s) 2292
Bsp1286I GDGCHC 7 cut(s) 374, 866, 979, 1746, 2745, 2989, 3520
BspCNI CTCAG 8 cut(s) 256, 801, 1248, 1569, 2443, 2490, 3003, 3567
BspDI ATCGAT 1 cut(s) 822
BspLI GGNNCC 4 cut(s) 976, 1776, 1811, 2098
BspMAI CTGCAG 5 cut(s) 117, 991, 1516, 2916, 3831
BspOI GCTAGC 1 cut(s) 3902
BspPI GGATC 5 cut(s) 64, 566, 3238, 3737, 3933
BspQI GCTCTTC 2 cut(s) 2574, 3426
BspT104I TTCGAA 1 cut(s) 2292
BspT107I GGYRCC 2 cut(s) 1774, 1809
BspTI CTTAAG 2 cut(s) 1082, 2258
BsrBI CCGCTC 2 cut(s) 2202, 3419
BsrDI GCAATG 6 cut(s) 678, 1060, 1114, 2081, 3329, 3807
BsrI ACTGG 8 cut(s) 307, 1229, 2430, 2483, 2775, 2869, 3013, 4019
BssECI CCNNGG 4 cut(s) 62, 73, 1446, 1770
BssNI GRCGYC 1 cut(s) 2336
Bst2UI CCWGG 4 cut(s) 74, 3491, 3962, 3999
BstACI GRCGYC 1 cut(s) 2336
BstAFI CTTAAG 2 cut(s) 1082, 2258
BstBAI YACGTR 2 cut(s) 867, 947
BstBI TTCGAA 1 cut(s) 2292
BstENI CCTNNNNNAGG 1 cut(s) 907
BstH2I RGCGCY 1 cut(s) 3835
BstHHI GCGC 1 cut(s) 3834
BstMAI GTCTC 6 cut(s) 122, 1613, 1792, 2813, 3458, 4050
BstMCI CGRYCG 1 cut(s) 4084
BstMWI GCNNNNNNNGC 7 cut(s) 146, 289, 983, 1505, 2026, 2150, 3258
BstNI CCWGG 4 cut(s) 74, 3491, 3962, 3999
BstNSI RCATGY 6 cut(s) 1049, 1860, 1864, 2886, 3430, 3498
BstPAI GACNNNNGTC 2 cut(s) 3345, 3969
BstSCI CCNGG 5 cut(s) 72, 3489, 3960, 3997, 4086
BstSFI CTRYAG 6 cut(s) 113, 987, 1512, 2912, 3733, 3827
BstSLI GKGCMC 1 cut(s) 2745
BstV2I GAAGAC 5 cut(s) 537, 646, 1916, 2153, 2499
BstX2I RGATCY 6 cut(s) 15, 69, 3230, 3355, 3742, 3748
BstXI CCANNNNNNTGG 2 cut(s) 2632, 3544
BstYI RGATCY 6 cut(s) 15, 69, 3230, 3355, 3742, 3748
Bsu15I ATCGAT 1 cut(s) 822
Bsu36I CCTNAGG 1 cut(s) 2498
BsuI GTATCC 1 cut(s) 3357
BsuTUI ATCGAT 1 cut(s) 822
BtgZI GCGATG 2 cut(s) 579, 4077
BtsI GCAGTG 2 cut(s) 12, 198
BtsIMutI CAGTG 9 cut(s) 12, 198, 261, 711, 2490, 2998, 3686, 3742, 4012
CaiI CAGNNNCTG 4 cut(s) 43, 710, 986, 3835
CfoI GCGC 1 cut(s) 3834
Cfr13I GGNCC 3 cut(s) 509, 2097, 4017
ClaI ATCGAT 1 cut(s) 822
CseI GACGC 2 cut(s) 2906, 3103
CsiI ACCWGGT 1 cut(s) 3997
Csp6I GTAC 7 cut(s) 317, 1232, 1732, 1810, 2996, 3116, 3208
CspCI CAANNNNNGTGG 2 cut(s) 2837, 2872
CviQI GTAC 7 cut(s) 317, 1232, 1732, 1810, 2996, 3116, 3208
DraI TTTAAA 1 cut(s) 1002
DraIII CACNNNGTG 1 cut(s) 2993
EaeI YGGCCR 5 cut(s) 2386, 2427, 3047, 3479, 3649
EciI GGCGGA 1 cut(s) 3753
Ecl136II GAGCTC 1 cut(s) 372
Eco24I GRGCYC 2 cut(s) 374, 979
Eco32I GATATC 2 cut(s) 1396, 2949
Eco47I GGWCC 2 cut(s) 509, 2097
Eco53kI GAGCTC 1 cut(s) 372
Eco57I CTGAAG 3 cut(s) 20, 2396, 3548
Eco72I CACGTG 2 cut(s) 867, 947
Eco81I CCTNAGG 1 cut(s) 2498
Eco88I CYCGRG 2 cut(s) 63, 1447
EcoICRI GAGCTC 1 cut(s) 372
EcoNI CCTNNNNNAGG 1 cut(s) 907
EcoO109I RGGNCCY 1 cut(s) 2097
EcoRII CCWGG 4 cut(s) 72, 3489, 3960, 3997
EcoRV GATATC 2 cut(s) 1396, 2949
EcoT22I ATGCAT 3 cut(s) 1479, 1862, 2576
EcoT38I GRGCYC 2 cut(s) 374, 979
FalI AAGNNNNNCTT 4 cut(s) 396, 428, 1262, 1294
FaqI GGGAC 3 cut(s) 660, 1390, 2110
FauI CCCGC 1 cut(s) 637
FbaI TGATCA 1 cut(s) 2715
FblI GTMKAC 1 cut(s) 793
FriOI GRGCYC 2 cut(s) 374, 979
FspBI CTAG 4 cut(s) 503, 3042, 3608, 3899
GlaI GCGC 1 cut(s) 3833
GsaI CCCAGC 2 cut(s) 983, 3186
GsuI CTGGAG 4 cut(s) 880, 1650, 2792, 3224
HaeII RGCGCY 1 cut(s) 3835
HapII CCGG 3 cut(s) 418, 1671, 4087
HgaI GACGC 2 cut(s) 2906, 3103
HhaI GCGC 1 cut(s) 3834
Hin1I GRCGYC 1 cut(s) 2336
Hin6I GCGC 1 cut(s) 3832
HinP1I GCGC 1 cut(s) 3832
HincII GTYRAC 6 cut(s) 57, 794, 1854, 2010, 2880, 3006
HindII GTYRAC 6 cut(s) 57, 794, 1854, 2010, 2880, 3006
HindIII AAGCTT 4 cut(s) 88, 290, 2255, 2843
HpaI GTTAAC 2 cut(s) 57, 3006
HpaII CCGG 3 cut(s) 418, 1671, 4087
HphI GGTGA 9 cut(s) 287, 745, 949, 1254, 1412, 1502, 3700, 3796, 3850
Hpy166II GTNNAC 9 cut(s) 57, 794, 1854, 2010, 2743, 2880, 2996, 3006, 3305
Hpy8I GTNNAC 9 cut(s) 57, 794, 1854, 2010, 2743, 2880, 2996, 3006, 3305
Hpy99I CGWCG 1 cut(s) 2922
HpyCH4IV ACGT 8 cut(s) 866, 946, 1150, 1838, 1880, 1948, 2336, 4044
HpyF10VI GCNNNNNNNGC 7 cut(s) 146, 289, 983, 1505, 2026, 2150, 3258
HpySE526I ACGT 8 cut(s) 866, 946, 1150, 1838, 1880, 1948, 2336, 4044
Hsp92I GRCGYC 1 cut(s) 2336
HspAI GCGC 1 cut(s) 3832
KpnI GGTACC 1 cut(s) 1813
Ksp22I TGATCA 1 cut(s) 2715
KspAI GTTAAC 2 cut(s) 57, 3006
LguI GCTCTTC 2 cut(s) 2574, 3426
MabI ACCWGGT 1 cut(s) 3997
MaeI CTAG 4 cut(s) 503, 3042, 3608, 3899
MaeII ACGT 8 cut(s) 866, 946, 1150, 1838, 1880, 1948, 2336, 4044
MaeIII GTNAC 8 cut(s) 733, 757, 937, 1096, 1400, 1580, 2766, 3296
MbiI CCGCTC 2 cut(s) 2202, 3419
MfeI CAATTG 3 cut(s) 1724, 2189, 3521
MflI RGATCY 6 cut(s) 15, 69, 3230, 3355, 3742, 3748
MhlI GDGCHC 7 cut(s) 374, 866, 979, 1746, 2745, 2989, 3520
MlsI TGGCCA 5 cut(s) 2388, 2429, 3049, 3481, 3651
MluNI TGGCCA 5 cut(s) 2388, 2429, 3049, 3481, 3651
MlyI GAGTC 8 cut(s) 618, 1000, 1561, 1859, 2005, 2948, 3323, 3699
MmeI TCCRAC 6 cut(s) 499, 2202, 2469, 3291, 3510, 4033
Mox20I TGGCCA 5 cut(s) 2388, 2429, 3049, 3481, 3651
Mph1103I ATGCAT 3 cut(s) 1479, 1862, 2576
MroXI GAANNNNTTC 1 cut(s) 2556
MscI TGGCCA 5 cut(s) 2388, 2429, 3049, 3481, 3651
MslI CAYNNNNRTG 4 cut(s) 266, 2091, 2748, 2887
Msp20I TGGCCA 5 cut(s) 2388, 2429, 3049, 3481, 3651
MspA1I CMGCKG 4 cut(s) 178, 986, 2724, 3976
MspCI CTTAAG 2 cut(s) 1082, 2258
MspI CCGG 3 cut(s) 418, 1671, 4087
MspR9I CCNGG 5 cut(s) 74, 3491, 3962, 3999, 4088
MunI CAATTG 3 cut(s) 1724, 2189, 3521
Mva1269I GAATGC 1 cut(s) 29
MvaI CCWGG 4 cut(s) 74, 3491, 3962, 3999
MwoI GCNNNNNNNGC 7 cut(s) 146, 289, 983, 1505, 2026, 2150, 3258
NciI CCSGG 1 cut(s) 4088
NheI GCTAGC 1 cut(s) 3898
NlaIV GGNNCC 4 cut(s) 976, 1776, 1811, 2098
NmeAIII GCCGAG 2 cut(s) 606, 745
NmuCI GTSAC 4 cut(s) 733, 937, 1400, 3296
NsiI ATGCAT 3 cut(s) 1479, 1862, 2576
NspI RCATGY 6 cut(s) 1049, 1860, 1864, 2886, 3430, 3498
NspV TTCGAA 1 cut(s) 2292
OliI CACNNNNGTG 1 cut(s) 2748
PaeI GCATGC 2 cut(s) 1864, 3498
PciI ACATGT 1 cut(s) 2882
PciSI GCTCTTC 2 cut(s) 2574, 3426
PcsI WCGNNNNNNNCGW 1 cut(s) 246
PctI GAATGC 1 cut(s) 29
PdmI GAANNNNTTC 1 cut(s) 2556
PfeI GAWTC 4 cut(s) 878, 2370, 2636, 3869
PfoI TCCNGGA 1 cut(s) 3960
PleI GAGTC 8 cut(s) 618, 1000, 1561, 1858, 2005, 2948, 3322, 3699
PmaCI CACGTG 2 cut(s) 867, 947
PmlI CACGTG 2 cut(s) 867, 947
PpsI GAGTC 8 cut(s) 618, 1000, 1561, 1858, 2005, 2948, 3322, 3699
Ppu21I YACGTR 2 cut(s) 867, 947
PpuMI RGGWCCY 1 cut(s) 2097
PscI ACATGT 1 cut(s) 2882
PshAI GACNNNNGTC 2 cut(s) 3345, 3969
PshBI ATTAAT 3 cut(s) 1331, 1832, 3636
PsiI TTATAA 1 cut(s) 2697
Psp124BI GAGCTC 1 cut(s) 374
Psp5II RGGWCCY 1 cut(s) 2097
Psp6I CCWGG 4 cut(s) 72, 3489, 3960, 3997
PspCI CACGTG 2 cut(s) 867, 947
PspFI CCCAGC 2 cut(s) 979, 3182
PspGI CCWGG 4 cut(s) 72, 3489, 3960, 3997
PspN4I GGNNCC 4 cut(s) 976, 1776, 1811, 2098
PspPI GGNCC 3 cut(s) 509, 2097, 4017
PspPPI RGGWCCY 1 cut(s) 2097
PstI CTGCAG 5 cut(s) 117, 991, 1516, 2916, 3831
PstNI CAGNNNCTG 4 cut(s) 43, 710, 986, 3835
PsuI RGATCY 6 cut(s) 15, 69, 3230, 3355, 3742, 3748
PvuII CAGCTG 1 cut(s) 178
RsaI GTAC 7 cut(s) 318, 1233, 1733, 1811, 2997, 3117, 3209
RsaNI GTAC 7 cut(s) 317, 1232, 1732, 1810, 2996, 3116, 3208
RseI CAYNNNNRTG 4 cut(s) 266, 2091, 2748, 2887
SacI GAGCTC 1 cut(s) 374
SalI GTCGAC 1 cut(s) 792
SapI GCTCTTC 2 cut(s) 2574, 3426
Sau96I GGNCC 3 cut(s) 509, 2097, 4017
ScaI AGTACT 3 cut(s) 1233, 1733, 3209
SchI GAGTC 8 cut(s) 618, 1000, 1561, 1859, 2005, 2948, 3323, 3699
ScrFI CCNGG 5 cut(s) 74, 3491, 3962, 3999, 4088
SduI GDGCHC 7 cut(s) 374, 866, 979, 1746, 2745, 2989, 3520
SexAI ACCWGGT 1 cut(s) 3997
SfcI CTRYAG 6 cut(s) 113, 987, 1512, 2912, 3733, 3827
SfuI TTCGAA 1 cut(s) 2292
SinI GGWCC 2 cut(s) 509, 2097
SmiMI CAYNNNNRTG 4 cut(s) 266, 2091, 2748, 2887
SmlI CTYRAG 8 cut(s) 287, 689, 1082, 1361, 1847, 2101, 2258, 2999
SmoI CTYRAG 8 cut(s) 287, 689, 1082, 1361, 1847, 2101, 2258, 2999
SphI GCATGC 2 cut(s) 1864, 3498
SspI AATATT 4 cut(s) 1409, 1887, 2794, 2932
SspMI CTAG 4 cut(s) 503, 3042, 3608, 3899
SstI GAGCTC 1 cut(s) 374
StyD4I CCNGG 5 cut(s) 72, 3489, 3960, 3997, 4086
TaiI ACGT 8 cut(s) 869, 949, 1153, 1841, 1883, 1951, 2339, 4047
TaqII GACCGA 2 cut(s) 1419, 1784
TatI WGTACW 4 cut(s) 1231, 1731, 2995, 3207
TauI GCSGC 3 cut(s) 29, 986, 2205
TfiI GAWTC 4 cut(s) 878, 2370, 2636, 3869
TscAI CASTG 9 cut(s) 12, 198, 268, 711, 2490, 2998, 3686, 3742, 4019
TseFI GTSAC 4 cut(s) 733, 937, 1400, 3296
Tsp45I GTSAC 4 cut(s) 733, 937, 1400, 3296
TspGWI ACGGA 1 cut(s) 1774
TspRI CASTG 9 cut(s) 12, 198, 268, 711, 2490, 2998, 3686, 3742, 4019
Vha464I CTTAAG 2 cut(s) 1082, 2258
VneI GTGCAC 1 cut(s) 2741
VpaK11BI GGWCC 2 cut(s) 509, 2097
VspI ATTAAT 3 cut(s) 1331, 1832, 3636
XagI CCTNNNNNAGG 1 cut(s) 907
XbaI TCTAGA 1 cut(s) 3607
XceI RCATGY 6 cut(s) 1049, 1860, 1864, 2886, 3430, 3498
XcmI CCANNNNNNNNNTGG 3 cut(s) 613, 3042, 3474
XmiI GTMKAC 1 cut(s) 793
XmnI GAANNNNTTC 1 cut(s) 2556
XspI CTAG 4 cut(s) 503, 3042, 3608, 3899
ZraI GACGTC 1 cut(s) 2337
ZrmI AGTACT 3 cut(s) 1233, 1733, 3209
Zsp2I ATGCAT 3 cut(s) 1479, 1862, 2576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.