RchiOBHm_Chr6g0244851

resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000105
Physical Location & Seq
Forward (+)
824744 .. 829889
5146 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21943

Sequence Viewer

Length: 2502 bp
ATGGACAAAATTGAATACATAGAGGATGAATATACAGGAGCTGCTATGATTCAAAGAAGTTTATGCAAAACAAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGGCACAATTAGAAAAATTGGCTGGAAGTCGAGACTGGTTTGGTCCAGGGAGCAGAATTATCATAACCACCACCGATATTCACTTGTTAGAGGCATATGATGTTGATGCTATATACGAGGCTACCACGTTAAATTTTGGTGAGGCTCTTCAACTCTTGAGTTTTAAGGCTTTCAAGAAAAGCTCCCCACCAGAAAATTATTCGGACCTGTGCATCGATATTTTAGAGTATGCTCAGGGGCTGCCGTTAGCTCTTGTGGTTATAGGTTCTTTTCTATGTGGTAGAAGCACCAATGAATGGGCTAGTGCAAGTGATCGACTAAAAAATGCACCAAATAGAGAAATTATTGATGTGCTTCAGATTAGTTTTGATGGTCTAGAGGACATAGAGAAAGAAATATTCCTACATATTGCTTGTCTTTACAAGGGGAAAGATTGGGATCGTGTGAGACAAATACTAGACTATTGTAAGCTGAACCCTGTCATTGGATTAAGAGTTCTTGCTGATAAATCTCTCATTACTACTTCGCACAACGAAGTGTCAATGCATGATTTGCTACAAGAAATGGGCCGGGAAATAGTTCGTCGAGAGTCTCCGAAAGAGCCAGGCAAACGTAGTAGATTATGGTCTCGTGAAGAAATCCACAATGTGCTGAAGAAAAATAAGGGAACAGAAGCAATCCAAGGCATGGTAATGGAGTGGTCTGAATTAGAAGTGGCTCATTGGAATCCAGAAGCCTTGTCAAATTTGTCTCAACTTATTCTTCTCCATATTCATAATGTGGACCTTCCTAAAGGCCTTACCTGTCTTCCTAATTCCTTGAGACTCCTGGAATGGCCTGGCTGTCCGTTAAGATCTCTCCCACGAACGTTCGAACCAGATGAACTTATTGAACTTAACTTGTGTCACAGCAATATTGAACATCTTTGGAAGGGAACAAAGAATTTTAACAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAACATTGTGGAGATCCCAGACCTTGCAGGTGTTCAGAATCTTGAGAGTTTAGAGCTCGAAGGATGTAAGAGTTTGGTAAGAATCCATCAATCCCTTGGATGTCTCAAAAAGCTTATTGTCCTGAATCTCAAAGACTGCAAAAGTCTTGAGAATCTGCCAGGTAGAATTGAAATGGAATCTCTTGAAAAATTAATTCTTTCTAACTGCTCCAAAATTAAAAAGATTCCCGAGTTTGATGGAAATATGGAGCGTCTGTCTGTGCTTCATCTTGACGGGACTGCCATTGAGGGACTGCCTGTTTCAATTGGACGCCTGAGTGGCCTTGTGTCATTGAATCTAAGTAAGTGCAGAAATCTTGTTTGTCTTCCAAGCACCATTGTTCATTTATGCAAAGTAGTTTGGGGATCTTTAAATAAGTTCTTGCCTTCTGGATTGCAAAAAGTGAATGGAGAGCCAATGAGTTTCAGCTTGCCTAGGCTGTGTAATTTAACAGATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCAATGAATTCAGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGTCTTCCTTCAGGCATTGGGTCGCTTTCCAAGCTTGAGTACTGTAAGTTGAAGAATTGCAAGAGACTTGAAGATCTGTCAGACCTTCCATTGAATAGTAACCTAATTTTAAAGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTTAACAGATTAGACGAATCATGTTTTGGTTCAGGTTTCAATTTCATGAATTGCGTCAATCTAAAGGGCAATCAAGGATGCAATAGCATGGCATTTGAAATGCTGAAGGCAGTCATGTATCAGGGACTCTCTAGTGAGACGAAAACTATTCGAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCGAAGTGTTGGGTGTTCAGTATGTGTAGAGCTGCCTCCAGATTGGGATAGCAGTAGCATGAAGCTCAATGGAGAAGAATTCGCTGGAAATATCCGAGAGCCTTTATTTTGCTTTCGTGATGAAGTTTGCCCGTTTGAGTTGGATCACCTGTGGCTATTGTATGTATCTCGTGATGGATACTTTGGTAGTACAGGGTGGTGGCATAACATTTGTGGTCAGCTTAATTTCTTATTTCATACCAATGAGCTGTATCCGAATGGGAATAAGTGTGAAGTCCGTCTGTGTGGAGTCCGTCTGATATATGAGCAAGACGTGCAAGAGTTGAACAAACAAACAACTCAATCAAGCAGGAGGATGTCTCCAGATGAGGATAGATTGATTGATTTAGACATTGCAGTTGATGGGGAAACTAGTGGCACTGGTAGGTGTGGTAGCAGAACTTGCACGCTTGAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

833

Amino Acids

93.74

Weight (kDa)

5.51

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 12 - 96 3.6e-06 NB-ARC domain
WHD_ROQ1 PF23282 164 - 233 2.4e-20 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 422 - 612 3e-11 Disease resistance protein RPS4B-like, leucine-rich repeats
C-JID PF20160 669 - 786 5.1e-07 C-JID domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000105)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00561 FvH4_2g00570
malus_domestica MD10G1006400.v1.1 MD10G1006800.v1.1 MD10G1007300.v1.1 MD12G1125200.v1.1 MD12G1125400.v1.1
prunus_persica Prupe.2G059200_v2.0.a1 Prupe.8G005200_v2.0.a1 Prupe.8G046600_v2.0.a1
pyrus_communis pycom10g00460 pycom12g12340 pycom12g12360 pycom14g00580
rosa_chinensis RchiOBHm_Chr1g0315021 RchiOBHm_Chr1g0319431 RchiOBHm_Chr1g0319441 RchiOBHm_Chr1g0321301 RchiOBHm_Chr1g0322041 RchiOBHm_Chr1g0322381 RchiOBHm_Chr1g0322421 RchiOBHm_Chr1g0322491 RchiOBHm_Chr1g0322521 RchiOBHm_Chr1g0322531 RchiOBHm_Chr1g0322651 RchiOBHm_Chr1g0322701 RchiOBHm_Chr1g0322711 RchiOBHm_Chr1g0322741 RchiOBHm_Chr1g0322781 RchiOBHm_Chr1g0322831 RchiOBHm_Chr1g0322941 RchiOBHm_Chr1g0322961 RchiOBHm_Chr1g0323161 RchiOBHm_Chr1g0323221 RchiOBHm_Chr1g0323401 RchiOBHm_Chr1g0323411 RchiOBHm_Chr1g0323501 RchiOBHm_Chr1g0323611 RchiOBHm_Chr1g0323761 RchiOBHm_Chr1g0328941 RchiOBHm_Chr1g0328951 RchiOBHm_Chr1g0329001 RchiOBHm_Chr2g0101061 RchiOBHm_Chr5g0055671 RchiOBHm_Chr6g0244821 RchiOBHm_Chr6g0244831 RchiOBHm_Chr6g0244851 RchiOBHm_Chr6g0247291 RchiOBHm_Chr6g0247321 RchiOBHm_Chr6g0247331 RchiOBHm_Chr6g0250501 RchiOBHm_Chr6g0250511 RchiOBHm_Chr6g0250551 RchiOBHm_Chr6g0250581 RchiOBHm_Chr6g0250621 RchiOBHm_Chr6g0250681 RchiOBHm_Chr6g0250801 RchiOBHm_Chr6g0250831 RchiOBHm_Chr6g0250871 RchiOBHm_Chr6g0250911 RchiOBHm_Chr6g0250931 RchiOBHm_Chr6g0251011
rosa_laevigata RLG00000002602 RLG00000013892 RLG00000015208 RLG00000015326 RLG00000015500 RLG00000015524 RLG00000015527 RLG00000015528 RLG00000015531 RLG00000015536 RLG00000016540 RLG00000017048 RLG00000023324 RLG00000024652 RLG00000030298 RLG00000030301 RLG00000030303 RLG00000030304 RLG00000030318 RLG00000030322 RLG00000030325 RLG00000030326 RLG00000030331 RLG00000030350 RLG00000030354 RLG00000030357 RLG00000030358 RLG00000030367 RLG00000030369 RLG00000030382 RLG00000030389 RLG00000035003
rosa_multiflora Rmu_sc0000252.1_g000011 Rmu_sc0000252.1_g000020 Rmu_sc0000252.1_g000029 Rmu_sc0000276.1_g000063 Rmu_sc0000504.1_g000023 Rmu_sc0000745.1_g000032 Rmu_sc0000745.1_g000041 Rmu_sc0000749.1_g000028 Rmu_sc0000749.1_g000035 Rmu_sc0000749.1_g000041 Rmu_sc0000749.1_g000052 Rmu_sc0000804.1_g000001 Rmu_sc0001168.1_g000012 Rmu_sc0001209.1_g000011 Rmu_sc0001209.1_g000022 Rmu_sc0001209.1_g000029 Rmu_sc0001209.1_g000031 Rmu_sc0001209.1_g000032 Rmu_sc0001932.1_g000010 Rmu_sc0001932.1_g000023 Rmu_sc0001932.1_g000028 Rmu_sc0002132.1_g000015 Rmu_sc0002132.1_g000031 Rmu_sc0002132.1_g000047 Rmu_sc0002263.1_g000015 Rmu_sc0002263.1_g000024 Rmu_sc0002263.1_g000028 Rmu_sc0002263.1_g000030 Rmu_sc0002263.1_g000039 Rmu_sc0002634.1_g000017 Rmu_sc0002634.1_g000024 Rmu_sc0002634.1_g000038 Rmu_sc0003226.1_g000025 Rmu_sc0003226.1_g000083 Rmu_sc0003226.1_g000084 Rmu_sc0003413.1_g000022 Rmu_sc0003413.1_g000023 Rmu_sc0003418.1_g000011 Rmu_sc0003418.1_g000012 Rmu_sc0003743.1_g000036 Rmu_sc0004185.1_g000005 Rmu_sc0004368.1_g000015 Rmu_sc0004368.1_g000031 Rmu_sc0004368.1_g000032 Rmu_sc0004439.1_g000008 Rmu_sc0006638.1_g000017 Rmu_sc0007485.1_g000009 Rmu_sc0007698.1_g000015 Rmu_sc0009428.1_g000002 Rmu_sc0012920.1_g000010 Rmu_sc0019291.1_g000002 Rmu_sc0026821.1_g000001
rosa_roxburghii Rroxscaffold_160G00433940 Rroxscaffold_180G00433550 Rroxscaffold_1G00024620 Rroxscaffold_2G00121250 Rroxscaffold_2G00141590 Rroxscaffold_2G00146640 Rroxscaffold_2G00146660 Rroxscaffold_4G00320080 Rroxscaffold_4G00321280 Rroxscaffold_4G00326440 Rroxscaffold_4G00326450 Rroxscaffold_4G00326500 Rroxscaffold_4G00326530 Rroxscaffold_4G00326610 Rroxscaffold_4G00326620 Rroxscaffold_4G00326770 Rroxscaffold_4G00326830 Rroxscaffold_4G00326870 Rroxscaffold_4G00326930 Rroxscaffold_4G00326940 Rroxscaffold_4G00326960 Rroxscaffold_4G00326980 Rroxscaffold_4G00327010 Rroxscaffold_4G00327110 Rroxscaffold_4G00327120 Rroxscaffold_4G00327140 Rroxscaffold_4G00327190 Rroxscaffold_4G00327200 Rroxscaffold_4G00327230 Rroxscaffold_4G00327260 Rroxscaffold_4G00327270 Rroxscaffold_4G00327320 Rroxscaffold_4G00327330 Rroxscaffold_7G00198650 Rroxscaffold_7G00214100 Rroxscaffold_7G00217130 Rroxscaffold_7G00217160 Rroxscaffold_7G00217180
rosa_rugosa Rorug01G0034500 Rorug01G0035700 Rorug01G0036000 Rorug01G0036100 Rorug01G0036200 Rorug01G0036700 Rorug01G0037900 Rorug01G0038000 Rorug01G0039000.1 Rorug01G0039400 Rorug02G0096400 Rorug02G0096500 Rorug02G0096600 Rorug02G0096700 Rorug02G0096800 Rorug05G0294500 Rorug05G0497900 Rorug05G0499300 Rorug05G0524600 Rorug06G0043700 Rorug06G0044200 RorugPtG0003400.1
rosa_samantha Rh1BG033300 Rh1BG046700 Rh1BG047600 Rh1BG109300 Rh2CG149400 Rh5CG398600 Rh5CG398700 Rh6DG004400 Rh6DG004700 Rh6DG004900 Rh6DG032100 Rh6DG032400
rosa_wichuraiana Rw0G001800 Rw0G020500 Rw0G020900 Rw1G003430 Rw1G003890 Rw1G004390 Rw1G004400 Rw1G004440 Rw1G004470 Rw1G004490 Rw1G004500 Rw1G004510 Rw1G004570 Rw1G004610 Rw1G004660 Rw1G004680 Rw1G004700 Rw1G004780 Rw1G004800 Rw1G004830 Rw1G005020 Rw2G011250 Rw2G024080 Rw2G024100 Rw3G022830 Rw6G000680 Rw6G000750 Rw6G000780 Rw6G000810 Rw6G000830 Rw6G000860 Rw6G002220 Rw6G002240 Rw6G003350 Rw6G003380 Rw6G003430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1106
Acc36I ACCTGC 1 cut(s) 1106
AccB7I CCANNNNNTGG 2 cut(s) 402, 793
AclI AACGTT 1 cut(s) 974
AclWI GGATC 5 cut(s) 108, 552, 1096, 1501, 2190
AcsI RAATTY 6 cut(s) 238, 850, 1048, 1631, 1843, 2117
AcuI CTGAAG 4 cut(s) 446, 779, 1680, 1970
AcyI GRCGYC 1 cut(s) 1399
AdeI CACNNNGTG 1 cut(s) 754
AfaI GTAC 3 cut(s) 1727, 1813, 2230
AfiI CCNNNNNNNGG 3 cut(s) 402, 590, 793
AhlI ACTAGT 1 cut(s) 2450
AjiI CACGTC 1 cut(s) 2353
AjnI CCWGG 6 cut(s) 151, 709, 933, 943, 1246, 2008
AjuI GAANNNNNNNTTGG 2 cut(s) 1854, 1886
AloI GAACNNNNNNTCC 2 cut(s) 585, 617
Alw21I GWGCWC 1 cut(s) 1146
AlwI GGATC 5 cut(s) 108, 552, 1096, 1501, 2190
AlwNI CAGNNNCTG 2 cut(s) 41, 346
Ama87I CYCGRG 1 cut(s) 1316
AoxI GGCC 4 cut(s) 673, 901, 941, 1408
ApeKI GCWGC 3 cut(s) 41, 346, 2071
ApoI RAATTY 6 cut(s) 238, 850, 1048, 1631, 1843, 2117
AseI ATTAAT 1 cut(s) 1280
Asp700I GAANNNNTTC 1 cut(s) 684
AspA2I CCTAGG 1 cut(s) 1562
AspS9I GGNCC 4 cut(s) 149, 310, 673, 889
AsuC2I CCSGG 1 cut(s) 677
AsuHPI GGTGA 4 cut(s) 257, 1622, 1666, 2177
AsuII TTCGAA 2 cut(s) 978, 1996
AvaI CYCGRG 1 cut(s) 1316
AvaII GGWCC 3 cut(s) 149, 310, 889
AvrII CCTAGG 1 cut(s) 1562
BaeI ACNNNNGTAYC 2 cut(s) 2209, 2242
BanII GRGCYC 1 cut(s) 1146
BauI CACGAG 2 cut(s) 735, 2208
BbsI GAAGAC 3 cut(s) 905, 1445, 1682
Bbv12I GWGCWC 1 cut(s) 1146
BbvI GCAGC 3 cut(s) 28, 333, 2058
BccI CCATC 7 cut(s) 470, 1182, 1319, 1799, 2046, 2207, 2435
BceAI ACGGC 1 cut(s) 334
BciT130I CCWGG 6 cut(s) 153, 711, 935, 945, 1248, 2010
BciVI GTATCC 2 cut(s) 2210, 2301
BcnI CCSGG 1 cut(s) 677
BcuI ACTAGT 1 cut(s) 2450
BfaI CTAG 6 cut(s) 408, 482, 563, 1563, 1977, 2451
BfuAI ACCTGC 1 cut(s) 1106
BfuI GTATCC 2 cut(s) 2210, 2301
BglI GCCNNNNNGGC 1 cut(s) 1407
BglII AGATCT 3 cut(s) 959, 1582, 1759
BisI GCNGC 3 cut(s) 42, 347, 2072
BlnI CCTAGG 1 cut(s) 1562
BlsI GCNGC 3 cut(s) 43, 348, 2073
BmcAI AGTACT 1 cut(s) 1727
Bme1390I CCNGG 7 cut(s) 153, 677, 711, 935, 945, 1248, 2010
Bme18I GGWCC 3 cut(s) 149, 310, 889
BmeT110I CYCGRG 1 cut(s) 1316
BmgBI CACGTC 1 cut(s) 2353
BmgT120I GGNCC 4 cut(s) 149, 310, 673, 889
BmrFI CCNGG 7 cut(s) 153, 677, 711, 935, 945, 1248, 2010
BmsI GCATC 5 cut(s) 202, 327, 1825, 1847, 1913
BpiI GAAGAC 3 cut(s) 905, 1445, 1682
BplI GAGNNNNNCTC 2 cut(s) 2383, 2415
BpmI CTGGAG 2 cut(s) 2061, 2385
Bpu10I CCTNAGC 1 cut(s) 339
Bpu14I TTCGAA 2 cut(s) 978, 1996
BpuEI CTTGAG 5 cut(s) 283, 946, 1151, 1256, 1742
BpuMI CCSGG 1 cut(s) 677
Bsa29I ATCGAT 1 cut(s) 321
BsaBI GATNNNNATC 2 cut(s) 99, 543
BsaHI GRCGYC 1 cut(s) 1399
BsaI GGTCTC 1 cut(s) 738
BsaJI CCNNGG 4 cut(s) 152, 787, 1183, 1562
BsaXI ACNNNNNCTCC 2 cut(s) 1328, 1358
Bsc4I CCNNNNNNNGG 3 cut(s) 402, 590, 793
Bse1I ACTGG 2 cut(s) 146, 2464
Bse3DI GCAATG 1 cut(s) 2430
Bse8I GATNNNNATC 2 cut(s) 99, 543
BseBI CCWGG 6 cut(s) 153, 711, 935, 945, 1248, 2010
BseCI ATCGAT 1 cut(s) 321
BseDI CCNNGG 4 cut(s) 152, 787, 1183, 1562
BseGI GGATG 5 cut(s) 31, 1157, 1193, 1928, 2400
BseJI GATNNNNATC 2 cut(s) 99, 543
BseLI CCNNNNNNNGG 3 cut(s) 402, 590, 793
BseMI GCAATG 1 cut(s) 2430
BseMII CTCAG 4 cut(s) 353, 1394, 1583, 2016
BseNI ACTGG 2 cut(s) 146, 2464
BseXI GCAGC 3 cut(s) 28, 333, 2058
BsgI GTGCAG 1 cut(s) 1456
BshFI GGCC 4 cut(s) 675, 903, 943, 1410
BshVI ATCGAT 1 cut(s) 321
BsiHKAI GWGCWC 1 cut(s) 1146
BsiHKCI CYCGRG 1 cut(s) 1316
BsiSI CCGG 1 cut(s) 676
BslFI GGGAC 3 cut(s) 1378, 1392, 1983
BslI CCNNNNNNNGG 3 cut(s) 402, 590, 793
BsmBI CGTCTC 1 cut(s) 1976
BsmFI GGGAC 3 cut(s) 1378, 1392, 1983
BsnI GGCC 4 cut(s) 675, 903, 943, 1410
Bso31I GGTCTC 1 cut(s) 738
BsoBI CYCGRG 1 cut(s) 1316
Bsp119I TTCGAA 2 cut(s) 978, 1996
Bsp1286I GDGCHC 1 cut(s) 1146
Bsp143I GATC 9 cut(s) 100, 418, 544, 959, 1101, 1493, 1582, 1759, 2182
BspANI GGCC 4 cut(s) 675, 903, 943, 1410
BspCNI CTCAG 4 cut(s) 352, 1395, 1584, 2017
BspDI ATCGAT 1 cut(s) 321
BspHI TCATGA 1 cut(s) 1890
BspMI ACCTGC 1 cut(s) 1106
BspPI GGATC 5 cut(s) 108, 552, 1096, 1501, 2190
BspQI GCTCTTC 1 cut(s) 258
BspT104I TTCGAA 2 cut(s) 978, 1996
BspTNI GGTCTC 1 cut(s) 738
BsrDI GCAATG 1 cut(s) 2430
BsrI ACTGG 2 cut(s) 146, 2464
BssECI CCNNGG 4 cut(s) 152, 787, 1183, 1562
BssMI GATC 9 cut(s) 100, 418, 544, 959, 1101, 1493, 1582, 1759, 2182
BssNI GRCGYC 1 cut(s) 1399
BssSI CACGAG 2 cut(s) 735, 2208
BssT1I CCWWGG 3 cut(s) 787, 1183, 1562
Bst2BI CACGAG 2 cut(s) 735, 2208
Bst2UI CCWGG 6 cut(s) 153, 711, 935, 945, 1248, 2010
Bst4CI ACNGT 1 cut(s) 1730
Bst6I CTCTTC 1 cut(s) 258
BstACI GRCGYC 1 cut(s) 1399
BstAPI GCANNNNNTGC 3 cut(s) 658, 2353, 2481
BstBI TTCGAA 2 cut(s) 978, 1996
BstC8I GCNNGC 2 cut(s) 1559, 2486
BstDEI CTNAG 6 cut(s) 339, 1403, 1427, 1592, 1667, 2025
BstEII GGTNACC 1 cut(s) 1654
BstF5I GGATG 5 cut(s) 31, 1157, 1193, 1928, 2400
BstKTI GATC 9 cut(s) 103, 421, 547, 962, 1104, 1496, 1585, 1762, 2185
BstMBI GATC 9 cut(s) 100, 418, 544, 959, 1101, 1493, 1582, 1759, 2182
BstMWI GCNNNNNNNGC 5 cut(s) 658, 1407, 1717, 2353, 2481
BstNI CCWGG 6 cut(s) 153, 711, 935, 945, 1248, 2010
BstPI GGTNACC 1 cut(s) 1654
BstSCI CCNGG 7 cut(s) 151, 675, 709, 933, 943, 1246, 2008
BstV1I GCAGC 3 cut(s) 28, 333, 2058
BstV2I GAAGAC 3 cut(s) 905, 1445, 1682
BstX2I RGATCY 5 cut(s) 959, 1101, 1493, 1582, 1759
BstYI RGATCY 5 cut(s) 959, 1101, 1493, 1582, 1759
Bsu15I ATCGAT 1 cut(s) 321
BsuI GTATCC 2 cut(s) 2210, 2301
BsuRI GGCC 4 cut(s) 675, 903, 943, 1410
BsuTUI ATCGAT 1 cut(s) 321
BtrI CACGTC 1 cut(s) 2353
BtsCI GGATG 5 cut(s) 31, 1157, 1193, 1928, 2400
BtsIMutI CAGTG 2 cut(s) 1817, 2457
BveI ACCTGC 1 cut(s) 1106
Cac8I GCNNGC 2 cut(s) 1559, 2486
CaiI CAGNNNCTG 2 cut(s) 41, 346
CciI TCATGA 1 cut(s) 1890
Cfr13I GGNCC 4 cut(s) 149, 310, 673, 889
ClaI ATCGAT 1 cut(s) 321
CseI GACGC 3 cut(s) 1328, 1407, 1888
Csp6I GTAC 3 cut(s) 1726, 1812, 2229
CviAII CATG 7 cut(s) 653, 793, 1866, 1891, 1933, 1960, 2098
CviQI GTAC 3 cut(s) 1726, 1812, 2229
DdeI CTNAG 6 cut(s) 339, 1403, 1427, 1592, 1667, 2025
DpnI GATC 9 cut(s) 102, 420, 546, 961, 1103, 1495, 1584, 1761, 2184
DpnII GATC 9 cut(s) 100, 418, 544, 959, 1101, 1493, 1582, 1759, 2182
DraI TTTAAA 2 cut(s) 1500, 1797
DraIII CACNNNGTG 1 cut(s) 754
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Ecl136II GAGCTC 1 cut(s) 1144
Eco130I CCWWGG 3 cut(s) 787, 1183, 1562
Eco147I AGGCCT 1 cut(s) 903
Eco24I GRGCYC 1 cut(s) 1146
Eco31I GGTCTC 1 cut(s) 738
Eco47I GGWCC 3 cut(s) 149, 310, 889
Eco53kI GAGCTC 1 cut(s) 1144
Eco57I CTGAAG 4 cut(s) 446, 779, 1680, 1970
Eco88I CYCGRG 1 cut(s) 1316
Eco91I GGTNACC 1 cut(s) 1654
EcoICRI GAGCTC 1 cut(s) 1144
EcoO65I GGTNACC 1 cut(s) 1654
EcoRI GAATTC 2 cut(s) 1631, 2117
EcoRII CCWGG 6 cut(s) 151, 709, 933, 943, 1246, 2008
EcoT14I CCWWGG 3 cut(s) 787, 1183, 1562
EcoT22I ATGCAT 2 cut(s) 654, 1840
EcoT38I GRGCYC 1 cut(s) 1146
ErhI CCWWGG 3 cut(s) 787, 1183, 1562
Esp3I CGTCTC 1 cut(s) 1976
FaeI CATG 7 cut(s) 656, 796, 1869, 1894, 1936, 1963, 2101
FaqI GGGAC 3 cut(s) 1378, 1392, 1983
FatI CATG 7 cut(s) 652, 792, 1865, 1890, 1932, 1959, 2097
FauNDI CATATG 1 cut(s) 202
Fnu4HI GCNGC 3 cut(s) 42, 347, 2072
FokI GGATG 5 cut(s) 38, 1164, 1200, 1935, 2407
FriOI GRGCYC 1 cut(s) 1146
Fsp4HI GCNGC 3 cut(s) 42, 347, 2072
FspBI CTAG 6 cut(s) 408, 482, 563, 1563, 1977, 2451
GluI GCNGC 3 cut(s) 42, 347, 2072
GsuI CTGGAG 2 cut(s) 2061, 2385
HaeIII GGCC 4 cut(s) 675, 903, 943, 1410
HapII CCGG 1 cut(s) 676
HgaI GACGC 3 cut(s) 1328, 1407, 1888
Hin1I GRCGYC 1 cut(s) 1399
Hin1II CATG 7 cut(s) 656, 796, 1869, 1894, 1936, 1963, 2101
HindIII AAGCTT 2 cut(s) 1199, 1718
HpaII CCGG 1 cut(s) 676
HphI GGTGA 4 cut(s) 257, 1622, 1666, 2177
Hpy166II GTNNAC 1 cut(s) 889
Hpy8I GTNNAC 1 cut(s) 889
Hpy99I CGWCG 1 cut(s) 693
HpyAV CCTTC 8 cut(s) 902, 1030, 1142, 1524, 1607, 1704, 1781, 1945
HpyCH4III ACNGT 1 cut(s) 1730
HpyCH4IV ACGT 4 cut(s) 233, 718, 974, 2352
HpyF10VI GCNNNNNNNGC 5 cut(s) 658, 1407, 1717, 2353, 2481
HpyF3I CTNAG 6 cut(s) 339, 1403, 1427, 1592, 1667, 2025
HpySE526I ACGT 4 cut(s) 233, 718, 974, 2352
Hsp92I GRCGYC 1 cut(s) 1399
Hsp92II CATG 7 cut(s) 656, 796, 1869, 1894, 1936, 1963, 2101
Kzo9I GATC 9 cut(s) 100, 418, 544, 959, 1101, 1493, 1582, 1759, 2182
LguI GCTCTTC 1 cut(s) 258
LmnI GCTCC 6 cut(s) 38, 156, 293, 1301, 1336, 1616
Lsp1109I GCAGC 3 cut(s) 28, 333, 2058
LweI GCATC 5 cut(s) 202, 327, 1825, 1847, 1913
MaeI CTAG 6 cut(s) 408, 482, 563, 1563, 1977, 2451
MaeII ACGT 4 cut(s) 233, 718, 974, 2352
MaeIII GTNAC 4 cut(s) 1010, 1637, 1654, 1784
MalI GATC 9 cut(s) 102, 420, 546, 961, 1103, 1495, 1584, 1761, 2184
MboI GATC 9 cut(s) 100, 418, 544, 959, 1101, 1493, 1582, 1759, 2182
MfeI CAATTG 1 cut(s) 1392
MflI RGATCY 5 cut(s) 959, 1101, 1493, 1582, 1759
MhlI GDGCHC 1 cut(s) 1146
MlyI GAGTC 4 cut(s) 704, 924, 1965, 2337
MmeI TCCRAC 1 cut(s) 2160
Mph1103I ATGCAT 2 cut(s) 654, 1840
MroXI GAANNNNTTC 1 cut(s) 684
MslI CAYNNNNRTG 3 cut(s) 797, 2043, 2280
MspI CCGG 1 cut(s) 676
MspR9I CCNGG 7 cut(s) 153, 677, 711, 935, 945, 1248, 2010
MunI CAATTG 1 cut(s) 1392
MvaI CCWGG 6 cut(s) 153, 711, 935, 945, 1248, 2010
MwoI GCNNNNNNNGC 5 cut(s) 658, 1407, 1717, 2353, 2481
NciI CCSGG 1 cut(s) 677
NdeI CATATG 1 cut(s) 202
NdeII GATC 9 cut(s) 100, 418, 544, 959, 1101, 1493, 1582, 1759, 2182
NlaIII CATG 7 cut(s) 656, 796, 1869, 1894, 1936, 1963, 2101
NmuCI GTSAC 2 cut(s) 1010, 1654
NsiI ATGCAT 2 cut(s) 654, 1840
NspV TTCGAA 2 cut(s) 978, 1996
PagI TCATGA 1 cut(s) 1890
PaqCI CACCTGC 1 cut(s) 1106
PceI AGGCCT 1 cut(s) 903
PciSI GCTCTTC 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 684
PflMI CCANNNNNTGG 2 cut(s) 402, 793
PfoI TCCNGGA 2 cut(s) 933, 2008
PkrI GCNGC 3 cut(s) 43, 348, 2073
PleI GAGTC 4 cut(s) 703, 924, 1965, 2336
PpsI GAGTC 4 cut(s) 703, 924, 1965, 2336
PshBI ATTAAT 1 cut(s) 1280
Psp124BI GAGCTC 1 cut(s) 1146
Psp1406I AACGTT 1 cut(s) 974
Psp6I CCWGG 6 cut(s) 151, 709, 933, 943, 1246, 2008
PspEI GGTNACC 1 cut(s) 1654
PspGI CCWGG 6 cut(s) 151, 709, 933, 943, 1246, 2008
PspPI GGNCC 4 cut(s) 149, 310, 673, 889
PstNI CAGNNNCTG 2 cut(s) 41, 346
PsuI RGATCY 5 cut(s) 959, 1101, 1493, 1582, 1759
RsaI GTAC 3 cut(s) 1727, 1813, 2230
RsaNI GTAC 3 cut(s) 1726, 1812, 2229
RseI CAYNNNNRTG 3 cut(s) 797, 2043, 2280
SacI GAGCTC 1 cut(s) 1146
SapI GCTCTTC 1 cut(s) 258
SatI GCNGC 3 cut(s) 42, 347, 2072
Sau3AI GATC 9 cut(s) 100, 418, 544, 959, 1101, 1493, 1582, 1759, 2182
Sau96I GGNCC 4 cut(s) 149, 310, 673, 889
ScaI AGTACT 1 cut(s) 1727
SchI GAGTC 4 cut(s) 704, 924, 1965, 2337
ScrFI CCNGG 7 cut(s) 153, 677, 711, 935, 945, 1248, 2010
SduI GDGCHC 1 cut(s) 1146
SfaNI GCATC 5 cut(s) 202, 327, 1825, 1847, 1913
SfuI TTCGAA 2 cut(s) 978, 1996
SinI GGWCC 3 cut(s) 149, 310, 889
SmiMI CAYNNNNRTG 3 cut(s) 797, 2043, 2280
SmlI CTYRAG 5 cut(s) 262, 925, 1130, 1235, 1721
SmoI CTYRAG 5 cut(s) 262, 925, 1130, 1235, 1721
SpeI ACTAGT 1 cut(s) 2450
SseBI AGGCCT 1 cut(s) 903
SspI AATATT 3 cut(s) 504, 1021, 2020
SspMI CTAG 6 cut(s) 408, 482, 563, 1563, 1977, 2451
SstI GAGCTC 1 cut(s) 1146
StuI AGGCCT 1 cut(s) 903
StyD4I CCNGG 7 cut(s) 151, 675, 709, 933, 943, 1246, 2008
StyI CCWWGG 3 cut(s) 787, 1183, 1562
TaaI ACNGT 1 cut(s) 1730
TaiI ACGT 4 cut(s) 236, 721, 977, 2355
TaqI TCGA 8 cut(s) 136, 321, 421, 691, 978, 1146, 1996, 2041
TatI WGTACW 3 cut(s) 1725, 1811, 2228
TscAI CASTG 2 cut(s) 1824, 2464
TseFI GTSAC 2 cut(s) 1010, 1654
TseI GCWGC 3 cut(s) 41, 346, 2071
Tsp45I GTSAC 2 cut(s) 1010, 1654
TspGWI ACGGA 3 cut(s) 942, 2306, 2321
TspRI CASTG 2 cut(s) 1824, 2464
Van91I CCANNNNNTGG 2 cut(s) 402, 793
VpaK11BI GGWCC 3 cut(s) 149, 310, 889
VspI ATTAAT 1 cut(s) 1280
XapI RAATTY 6 cut(s) 238, 850, 1048, 1631, 1843, 2117
XbaI TCTAGA 1 cut(s) 481
XcmI CCANNNNNNNNNTGG 2 cut(s) 1181, 2045
XmaJI CCTAGG 1 cut(s) 1562
XmnI GAANNNNTTC 1 cut(s) 684
XspI CTAG 6 cut(s) 408, 482, 563, 1563, 1977, 2451
ZrmI AGTACT 1 cut(s) 1727
Zsp2I ATGCAT 2 cut(s) 654, 1840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.