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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 125, 220 |
| AcyI | GRCGYC | 1 cut(s) | 53 |
| AdeI | CACNNNGTG | 1 cut(s) | 268 |
| AfaI | GTAC | 1 cut(s) | 281 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 43, 164 |
| AjnI | CCWGG | 3 cut(s) | 48, 102, 327 |
| Alw21I | GWGCWC | 1 cut(s) | 264 |
| Alw26I | GTCTC | 2 cut(s) | 121, 218 |
| Alw44I | GTGCAC | 1 cut(s) | 260 |
| AoxI | GGCC | 1 cut(s) | 154 |
| ApaLI | GTGCAC | 1 cut(s) | 260 |
| AspS9I | GGNCC | 3 cut(s) | 46, 155, 222 |
| AsuHPI | GGTGA | 3 cut(s) | 48, 242, 376 |
| AvaII | GGWCC | 2 cut(s) | 46, 222 |
| BaeGI | GKGCMC | 2 cut(s) | 195, 264 |
| BauI | CACGAG | 1 cut(s) | 197 |
| Bbv12I | GWGCWC | 1 cut(s) | 264 |
| BccI | CCATC | 1 cut(s) | 115 |
| BceAI | ACGGC | 1 cut(s) | 56 |
| BciT130I | CCWGG | 3 cut(s) | 50, 104, 329 |
| BcoDI | GTCTC | 2 cut(s) | 121, 218 |
| BglII | AGATCT | 1 cut(s) | 274 |
| BisI | GCNGC | 1 cut(s) | 126 |
| BlsI | GCNGC | 1 cut(s) | 127 |
| Bme1390I | CCNGG | 3 cut(s) | 50, 104, 329 |
| Bme18I | GGWCC | 2 cut(s) | 46, 222 |
| BmgT120I | GGNCC | 3 cut(s) | 46, 155, 222 |
| BmiI | GGNNCC | 2 cut(s) | 47, 157 |
| BmrFI | CCNGG | 3 cut(s) | 50, 104, 329 |
| BmrI | ACTGGG | 1 cut(s) | 378 |
| BmsI | GCATC | 2 cut(s) | 192, 331 |
| BmuI | ACTGGG | 1 cut(s) | 378 |
| Bpu10I | CCTNAGC | 1 cut(s) | 345 |
| BsaHI | GRCGYC | 1 cut(s) | 53 |
| BsaI | GGTCTC | 1 cut(s) | 218 |
| BsaJI | CCNNGG | 3 cut(s) | 132, 225, 328 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 43, 164 |
| Bse1I | ACTGG | 1 cut(s) | 373 |
| Bse3DI | GCAATG | 1 cut(s) | 240 |
| BseBI | CCWGG | 3 cut(s) | 50, 104, 329 |
| BseDI | CCNNGG | 3 cut(s) | 132, 225, 328 |
| BseGI | GGATG | 1 cut(s) | 183 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 43, 164 |
| BseMI | GCAATG | 1 cut(s) | 240 |
| BseMII | CTCAG | 1 cut(s) | 359 |
| BseNI | ACTGG | 1 cut(s) | 373 |
| BseSI | GKGCMC | 2 cut(s) | 195, 264 |
| Bsh1285I | CGRYCG | 1 cut(s) | 132 |
| BshFI | GGCC | 1 cut(s) | 156 |
| BsiEI | CGRYCG | 1 cut(s) | 132 |
| BsiHKAI | GWGCWC | 1 cut(s) | 264 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 43, 164 |
| BsmAI | GTCTC | 2 cut(s) | 121, 218 |
| BsnI | GGCC | 1 cut(s) | 156 |
| Bso31I | GGTCTC | 1 cut(s) | 218 |
| Bsp1286I | GDGCHC | 2 cut(s) | 195, 264 |
| Bsp1407I | TGTACA | 1 cut(s) | 279 |
| Bsp143I | GATC | 2 cut(s) | 255, 274 |
| BspACI | CCGC | 2 cut(s) | 125, 220 |
| BspANI | GGCC | 1 cut(s) | 156 |
| BspCNI | CTCAG | 1 cut(s) | 358 |
| BspLI | GGNNCC | 2 cut(s) | 47, 157 |
| BspTNI | GGTCTC | 1 cut(s) | 218 |
| BsrDI | GCAATG | 1 cut(s) | 240 |
| BsrGI | TGTACA | 1 cut(s) | 279 |
| BsrI | ACTGG | 1 cut(s) | 373 |
| BssECI | CCNNGG | 3 cut(s) | 132, 225, 328 |
| BssMI | GATC | 2 cut(s) | 255, 274 |
| BssNI | GRCGYC | 1 cut(s) | 53 |
| BssSI | CACGAG | 1 cut(s) | 197 |
| BssT1I | CCWWGG | 1 cut(s) | 225 |
| Bst2BI | CACGAG | 1 cut(s) | 197 |
| Bst2UI | CCWGG | 3 cut(s) | 50, 104, 329 |
| Bst4CI | ACNGT | 2 cut(s) | 167, 299 |
| BstACI | GRCGYC | 1 cut(s) | 53 |
| BstAUI | TGTACA | 1 cut(s) | 279 |
| BstDEI | CTNAG | 1 cut(s) | 345 |
| BstF5I | GGATG | 1 cut(s) | 183 |
| BstKTI | GATC | 2 cut(s) | 258, 277 |
| BstMAI | GTCTC | 2 cut(s) | 121, 218 |
| BstMBI | GATC | 2 cut(s) | 255, 274 |
| BstMCI | CGRYCG | 1 cut(s) | 132 |
| BstNI | CCWGG | 3 cut(s) | 50, 104, 329 |
| BstSCI | CCNGG | 3 cut(s) | 48, 102, 327 |
| BstSLI | GKGCMC | 2 cut(s) | 195, 264 |
| BstX2I | RGATCY | 1 cut(s) | 274 |
| BstYI | RGATCY | 1 cut(s) | 274 |
| BsuRI | GGCC | 1 cut(s) | 156 |
| BtgZI | GCGATG | 2 cut(s) | 52, 275 |
| BtsCI | GGATG | 1 cut(s) | 183 |
| Cfr13I | GGNCC | 3 cut(s) | 46, 155, 222 |
| CseI | GACGC | 1 cut(s) | 42 |
| Csp6I | GTAC | 1 cut(s) | 280 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 148, 183 |
| CviAII | CATG | 1 cut(s) | 265 |
| CviJI | RGCY | 2 cut(s) | 102, 156 |
| CviKI_1 | RGCY | 2 cut(s) | 102, 156 |
| CviQI | GTAC | 1 cut(s) | 280 |
| DdeI | CTNAG | 1 cut(s) | 345 |
| DpnI | GATC | 2 cut(s) | 257, 276 |
| DpnII | GATC | 2 cut(s) | 255, 274 |
| DraIII | CACNNNGTG | 1 cut(s) | 268 |
| Eco130I | CCWWGG | 1 cut(s) | 225 |
| Eco31I | GGTCTC | 1 cut(s) | 218 |
| Eco32I | GATATC | 1 cut(s) | 41 |
| Eco47I | GGWCC | 2 cut(s) | 46, 222 |
| EcoO109I | RGGNCCY | 2 cut(s) | 46, 155 |
| EcoRII | CCWGG | 3 cut(s) | 48, 102, 327 |
| EcoRV | GATATC | 1 cut(s) | 41 |
| EcoT14I | CCWWGG | 1 cut(s) | 225 |
| ErhI | CCWWGG | 1 cut(s) | 225 |
| FaeI | CATG | 1 cut(s) | 268 |
| FaiI | YATR | 1 cut(s) | 266 |
| FatI | CATG | 1 cut(s) | 264 |
| Fnu4HI | GCNGC | 1 cut(s) | 126 |
| FokI | GGATG | 1 cut(s) | 170 |
| Fsp4HI | GCNGC | 1 cut(s) | 126 |
| GluI | GCNGC | 1 cut(s) | 126 |
| HaeIII | GGCC | 1 cut(s) | 156 |
| HgaI | GACGC | 1 cut(s) | 42 |
| Hin1I | GRCGYC | 1 cut(s) | 53 |
| Hin1II | CATG | 1 cut(s) | 268 |
| HincII | GTYRAC | 1 cut(s) | 310 |
| HindII | GTYRAC | 1 cut(s) | 310 |
| HpaI | GTTAAC | 1 cut(s) | 310 |
| HphI | GGTGA | 3 cut(s) | 48, 242, 376 |
| Hpy166II | GTNNAC | 3 cut(s) | 262, 310, 316 |
| Hpy188I | TCNGA | 2 cut(s) | 209, 274 |
| Hpy188III | TCNNGA | 3 cut(s) | 118, 338, 347 |
| Hpy8I | GTNNAC | 3 cut(s) | 262, 310, 316 |
| HpyCH4III | ACNGT | 2 cut(s) | 167, 299 |
| HpyCH4IV | ACGT | 1 cut(s) | 312 |
| HpyCH4V | TGCA | 2 cut(s) | 151, 262 |
| HpyF3I | CTNAG | 1 cut(s) | 345 |
| HpySE526I | ACGT | 1 cut(s) | 312 |
| Hsp92I | GRCGYC | 1 cut(s) | 53 |
| Hsp92II | CATG | 1 cut(s) | 268 |
| KspAI | GTTAAC | 1 cut(s) | 310 |
| Kzo9I | GATC | 2 cut(s) | 255, 274 |
| LpnPI | CCDG | 9 cut(s) | 35, 62, 72, 89, 116, 314, 332, 341, 354 |
| LweI | GCATC | 2 cut(s) | 192, 331 |
| MaeII | ACGT | 1 cut(s) | 312 |
| MaeIII | GTNAC | 4 cut(s) | 54, 94, 173, 364 |
| MalI | GATC | 2 cut(s) | 257, 276 |
| MboI | GATC | 2 cut(s) | 255, 274 |
| MfeI | CAATTG | 1 cut(s) | 283 |
| MflI | RGATCY | 1 cut(s) | 274 |
| MhlI | GDGCHC | 2 cut(s) | 195, 264 |
| MluCI | AATT | 2 cut(s) | 283, 319 |
| MmeI | TCCRAC | 1 cut(s) | 187 |
| MnlI | CCTC | 8 cut(s) | 15, 79, 127, 172, 193, 203, 345, 354 |
| MseI | TTAA | 1 cut(s) | 309 |
| MslI | CAYNNNNRTG | 1 cut(s) | 188 |
| MspR9I | CCNGG | 3 cut(s) | 50, 104, 329 |
| MunI | CAATTG | 1 cut(s) | 283 |
| MvaI | CCWGG | 3 cut(s) | 50, 104, 329 |
| NdeII | GATC | 2 cut(s) | 255, 274 |
| NlaIII | CATG | 1 cut(s) | 268 |
| NlaIV | GGNNCC | 2 cut(s) | 47, 157 |
| NmuCI | GTSAC | 4 cut(s) | 54, 94, 173, 364 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 125 |
| PkrI | GCNGC | 1 cut(s) | 127 |
| PpuMI | RGGWCCY | 1 cut(s) | 46 |
| Psp5II | RGGWCCY | 1 cut(s) | 46 |
| Psp6I | CCWGG | 3 cut(s) | 48, 102, 327 |
| PspGI | CCWGG | 3 cut(s) | 48, 102, 327 |
| PspN4I | GGNNCC | 2 cut(s) | 47, 157 |
| PspPI | GGNCC | 3 cut(s) | 46, 155, 222 |
| PspPPI | RGGWCCY | 1 cut(s) | 46 |
| PsuI | RGATCY | 1 cut(s) | 274 |
| RsaI | GTAC | 1 cut(s) | 281 |
| RsaNI | GTAC | 1 cut(s) | 280 |
| RseI | CAYNNNNRTG | 1 cut(s) | 188 |
| SaqAI | TTAA | 1 cut(s) | 309 |
| SatI | GCNGC | 1 cut(s) | 126 |
| Sau3AI | GATC | 2 cut(s) | 255, 274 |
| Sau96I | GGNCC | 3 cut(s) | 46, 155, 222 |
| ScrFI | CCNGG | 3 cut(s) | 50, 104, 329 |
| SduI | GDGCHC | 2 cut(s) | 195, 264 |
| SetI | ASST | 7 cut(s) | 108, 138, 204, 214, 227, 315, 356 |
| SfaNI | GCATC | 2 cut(s) | 192, 331 |
| SinI | GGWCC | 2 cut(s) | 46, 222 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 188 |
| Sse9I | AATT | 2 cut(s) | 283, 319 |
| SsiI | CCGC | 2 cut(s) | 125, 220 |
| StyD4I | CCNGG | 3 cut(s) | 48, 102, 327 |
| StyI | CCWWGG | 1 cut(s) | 225 |
| TaaI | ACNGT | 2 cut(s) | 167, 299 |
| TaiI | ACGT | 1 cut(s) | 315 |
| TaqI | TCGA | 2 cut(s) | 119, 337 |
| TaqII | GACCGA | 1 cut(s) | 146 |
| TasI | AATT | 2 cut(s) | 283, 319 |
| TatI | WGTACW | 1 cut(s) | 279 |
| TauI | GCSGC | 1 cut(s) | 128 |
| Tru1I | TTAA | 1 cut(s) | 309 |
| Tru9I | TTAA | 1 cut(s) | 309 |
| TseFI | GTSAC | 4 cut(s) | 54, 94, 173, 364 |
| Tsp45I | GTSAC | 4 cut(s) | 54, 94, 173, 364 |
| VneI | GTGCAC | 1 cut(s) | 260 |
| VpaK11BI | GGWCC | 2 cut(s) | 46, 222 |