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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 3 cut(s) | 295, 446, 448 |
| AciI | CCGC | 3 cut(s) | 218, 398, 408 |
| AclWI | GGATC | 3 cut(s) | 81, 270, 446 |
| AcoI | YGGCCR | 1 cut(s) | 116 |
| AdeI | CACNNNGTG | 1 cut(s) | 226 |
| AfeI | AGCGCT | 1 cut(s) | 371 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 61, 120, 223, 285, 397 |
| AluBI | AGCT | 1 cut(s) | 364 |
| AluI | AGCT | 1 cut(s) | 364 |
| Alw26I | GTCTC | 3 cut(s) | 99, 144, 406 |
| AlwI | GGATC | 3 cut(s) | 81, 270, 446 |
| Ama87I | CYCGRG | 4 cut(s) | 96, 280, 301, 403 |
| Aor51HI | AGCGCT | 1 cut(s) | 371 |
| AoxI | GGCC | 3 cut(s) | 116, 169, 475 |
| ApeKI | GCWGC | 1 cut(s) | 7 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 325 |
| AspLEI | GCGC | 2 cut(s) | 372, 448 |
| AspS9I | GGNCC | 1 cut(s) | 123 |
| AsuC2I | CCSGG | 1 cut(s) | 115 |
| AsuHPI | GGTGA | 2 cut(s) | 76, 200 |
| AvaI | CYCGRG | 4 cut(s) | 96, 280, 301, 403 |
| AvaII | GGWCC | 1 cut(s) | 123 |
| BccI | CCATC | 3 cut(s) | 54, 98, 480 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 438, 472 |
| BcnI | CCSGG | 1 cut(s) | 115 |
| BcoDI | GTCTC | 3 cut(s) | 99, 144, 406 |
| BfaI | CTAG | 2 cut(s) | 11, 365 |
| BfoI | RGCGCY | 1 cut(s) | 373 |
| BisI | GCNGC | 2 cut(s) | 8, 409 |
| BlsI | GCNGC | 2 cut(s) | 9, 410 |
| Bme1390I | CCNGG | 1 cut(s) | 115 |
| Bme18I | GGWCC | 1 cut(s) | 123 |
| BmeT110I | CYCGRG | 4 cut(s) | 96, 280, 301, 403 |
| BmgT120I | GGNCC | 1 cut(s) | 123 |
| BmrFI | CCNGG | 1 cut(s) | 115 |
| BmsI | GCATC | 2 cut(s) | 287, 346 |
| BpuMI | CCSGG | 1 cut(s) | 115 |
| Bsa29I | ATCGAT | 1 cut(s) | 505 |
| BsaI | GGTCTC | 3 cut(s) | 99, 144, 406 |
| BsaJI | CCNNGG | 1 cut(s) | 119 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 61, 120, 223, 285, 397 |
| Bse118I | RCCGGY | 1 cut(s) | 340 |
| BseCI | ATCGAT | 1 cut(s) | 505 |
| BseDI | CCNNGG | 1 cut(s) | 119 |
| BseGI | GGATG | 2 cut(s) | 361, 491 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 61, 120, 223, 285, 397 |
| BseX3I | CGGCCG | 1 cut(s) | 116 |
| Bsh1236I | CGCG | 3 cut(s) | 295, 446, 448 |
| Bsh1285I | CGRYCG | 1 cut(s) | 119 |
| BshFI | GGCC | 3 cut(s) | 118, 171, 477 |
| BshVI | ATCGAT | 1 cut(s) | 505 |
| BsiEI | CGRYCG | 1 cut(s) | 119 |
| BsiHKCI | CYCGRG | 4 cut(s) | 96, 280, 301, 403 |
| BsiSI | CCGG | 2 cut(s) | 115, 341 |
| BslFI | GGGAC | 2 cut(s) | 38, 200 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 61, 120, 223, 285, 397 |
| BsmAI | GTCTC | 3 cut(s) | 99, 144, 406 |
| BsmFI | GGGAC | 2 cut(s) | 38, 200 |
| BsnI | GGCC | 3 cut(s) | 118, 171, 477 |
| Bso31I | GGTCTC | 3 cut(s) | 99, 144, 406 |
| BsoBI | CYCGRG | 4 cut(s) | 96, 280, 301, 403 |
| Bsp143I | GATC | 4 cut(s) | 73, 252, 275, 451 |
| Bsp68I | TCGCGA | 1 cut(s) | 295 |
| BspACI | CCGC | 3 cut(s) | 218, 398, 408 |
| BspANI | GGCC | 3 cut(s) | 118, 171, 477 |
| BspDI | ATCGAT | 1 cut(s) | 505 |
| BspFNI | CGCG | 3 cut(s) | 295, 446, 448 |
| BspHI | TCATGA | 1 cut(s) | 151 |
| BspPI | GGATC | 3 cut(s) | 81, 270, 446 |
| BspTNI | GGTCTC | 3 cut(s) | 99, 144, 406 |
| BsrFI | RCCGGY | 1 cut(s) | 340 |
| BssAI | RCCGGY | 1 cut(s) | 340 |
| BssECI | CCNNGG | 1 cut(s) | 119 |
| BssMI | GATC | 4 cut(s) | 73, 252, 275, 451 |
| Bst4CI | ACNGT | 1 cut(s) | 244 |
| Bst6I | CTCTTC | 1 cut(s) | 16 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 498 |
| BstC8I | GCNNGC | 2 cut(s) | 440, 444 |
| BstDEI | CTNAG | 1 cut(s) | 46 |
| BstF5I | GGATG | 2 cut(s) | 361, 491 |
| BstFNI | CGCG | 3 cut(s) | 295, 446, 448 |
| BstH2I | RGCGCY | 1 cut(s) | 373 |
| BstHHI | GCGC | 2 cut(s) | 372, 448 |
| BstKTI | GATC | 4 cut(s) | 76, 255, 278, 454 |
| BstMAI | GTCTC | 3 cut(s) | 99, 144, 406 |
| BstMBI | GATC | 4 cut(s) | 73, 252, 275, 451 |
| BstMCI | CGRYCG | 1 cut(s) | 119 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 498 |
| BstNSI | RCATGY | 1 cut(s) | 442 |
| BstSCI | CCNGG | 1 cut(s) | 113 |
| BstUI | CGCG | 3 cut(s) | 295, 446, 448 |
| BstX2I | RGATCY | 3 cut(s) | 73, 275, 451 |
| BstYI | RGATCY | 3 cut(s) | 73, 275, 451 |
| BstZI | CGGCCG | 1 cut(s) | 116 |
| Bsu15I | ATCGAT | 1 cut(s) | 505 |
| BsuRI | GGCC | 3 cut(s) | 118, 171, 477 |
| BsuTUI | ATCGAT | 1 cut(s) | 505 |
| BtgZI | GCGATG | 1 cut(s) | 310 |
| BtsCI | GGATG | 2 cut(s) | 361, 491 |
| BtuMI | TCGCGA | 1 cut(s) | 295 |
| Cac8I | GCNNGC | 2 cut(s) | 440, 444 |
| CciI | TCATGA | 1 cut(s) | 151 |
| CfoI | GCGC | 2 cut(s) | 372, 448 |
| Cfr10I | RCCGGY | 1 cut(s) | 340 |
| Cfr13I | GGNCC | 1 cut(s) | 123 |
| ClaI | ATCGAT | 1 cut(s) | 505 |
| CviAII | CATG | 6 cut(s) | 80, 152, 223, 439, 456, 498 |
| CviJI | RGCY | 7 cut(s) | 113, 118, 171, 364, 390, 411, 477 |
| CviKI_1 | RGCY | 7 cut(s) | 113, 118, 171, 364, 390, 411, 477 |
| DdeI | CTNAG | 1 cut(s) | 46 |
| DpnI | GATC | 4 cut(s) | 75, 254, 277, 453 |
| DpnII | GATC | 4 cut(s) | 73, 252, 275, 451 |
| DraIII | CACNNNGTG | 1 cut(s) | 226 |
| EaeI | YGGCCR | 1 cut(s) | 116 |
| EagI | CGGCCG | 1 cut(s) | 116 |
| Eam1104I | CTCTTC | 1 cut(s) | 16 |
| EarI | CTCTTC | 1 cut(s) | 16 |
| EclXI | CGGCCG | 1 cut(s) | 116 |
| Eco147I | AGGCCT | 1 cut(s) | 171 |
| Eco31I | GGTCTC | 3 cut(s) | 99, 144, 406 |
| Eco47I | GGWCC | 1 cut(s) | 123 |
| Eco47III | AGCGCT | 1 cut(s) | 371 |
| Eco52I | CGGCCG | 1 cut(s) | 116 |
| Eco88I | CYCGRG | 4 cut(s) | 96, 280, 301, 403 |
| FaeI | CATG | 6 cut(s) | 83, 155, 226, 442, 459, 501 |
| FaiI | YATR | 7 cut(s) | 81, 153, 224, 335, 440, 457, 499 |
| FaqI | GGGAC | 2 cut(s) | 38, 200 |
| FatI | CATG | 6 cut(s) | 79, 151, 222, 438, 455, 497 |
| FauI | CCCGC | 1 cut(s) | 225 |
| Fnu4HI | GCNGC | 2 cut(s) | 8, 409 |
| FokI | GGATG | 2 cut(s) | 368, 498 |
| Fsp4HI | GCNGC | 2 cut(s) | 8, 409 |
| FspBI | CTAG | 2 cut(s) | 11, 365 |
| GlaI | GCGC | 2 cut(s) | 371, 447 |
| GluI | GCNGC | 2 cut(s) | 8, 409 |
| HaeII | RGCGCY | 1 cut(s) | 373 |
| HaeIII | GGCC | 3 cut(s) | 118, 171, 477 |
| HapII | CCGG | 2 cut(s) | 115, 341 |
| HhaI | GCGC | 2 cut(s) | 372, 448 |
| Hin1II | CATG | 6 cut(s) | 83, 155, 226, 442, 459, 501 |
| Hin6I | GCGC | 2 cut(s) | 370, 446 |
| HinP1I | GCGC | 2 cut(s) | 370, 446 |
| HinfI | GANTC | 2 cut(s) | 51, 283 |
| HpaII | CCGG | 2 cut(s) | 115, 341 |
| HphI | GGTGA | 2 cut(s) | 76, 200 |
| Hpy166II | GTNNAC | 1 cut(s) | 126 |
| Hpy188I | TCNGA | 2 cut(s) | 17, 167 |
| Hpy188III | TCNNGA | 5 cut(s) | 141, 152, 250, 294, 303 |
| Hpy8I | GTNNAC | 1 cut(s) | 126 |
| Hpy99I | CGWCG | 1 cut(s) | 438 |
| HpyAV | CCTTC | 1 cut(s) | 374 |
| HpyCH4III | ACNGT | 1 cut(s) | 244 |
| HpyCH4IV | ACGT | 1 cut(s) | 210 |
| HpyCH4V | TGCA | 2 cut(s) | 459, 492 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 498 |
| HpyF3I | CTNAG | 1 cut(s) | 46 |
| HpySE526I | ACGT | 1 cut(s) | 210 |
| Hsp92II | CATG | 6 cut(s) | 83, 155, 226, 442, 459, 501 |
| HspAI | GCGC | 2 cut(s) | 370, 446 |
| Kzo9I | GATC | 4 cut(s) | 73, 252, 275, 451 |
| LpnPI | CCDG | 2 cut(s) | 128, 354 |
| LweI | GCATC | 2 cut(s) | 287, 346 |
| MaeI | CTAG | 2 cut(s) | 11, 365 |
| MaeII | ACGT | 1 cut(s) | 210 |
| MalI | GATC | 4 cut(s) | 75, 254, 277, 453 |
| MboI | GATC | 4 cut(s) | 73, 252, 275, 451 |
| MboII | GAAGA | 3 cut(s) | 33, 68, 285 |
| MflI | RGATCY | 3 cut(s) | 73, 275, 451 |
| MluCI | AATT | 1 cut(s) | 192 |
| MlyI | GAGTC | 2 cut(s) | 60, 292 |
| MmeI | TCCRAC | 1 cut(s) | 145 |
| MroXI | GAANNNNTTC | 1 cut(s) | 325 |
| MseI | TTAA | 1 cut(s) | 266 |
| MslI | CAYNNNNRTG | 1 cut(s) | 150 |
| MspI | CCGG | 2 cut(s) | 115, 341 |
| MspR9I | CCNGG | 1 cut(s) | 115 |
| MvnI | CGCG | 3 cut(s) | 295, 446, 448 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 498 |
| NciI | CCSGG | 1 cut(s) | 115 |
| NdeII | GATC | 4 cut(s) | 73, 252, 275, 451 |
| NlaIII | CATG | 6 cut(s) | 83, 155, 226, 442, 459, 501 |
| NmeAIII | GCCGAG | 2 cut(s) | 144, 416 |
| NruI | TCGCGA | 1 cut(s) | 295 |
| NspI | RCATGY | 1 cut(s) | 442 |
| PaeI | GCATGC | 1 cut(s) | 442 |
| PaeR7I | CTCGAG | 1 cut(s) | 280 |
| PagI | TCATGA | 1 cut(s) | 151 |
| PceI | AGGCCT | 1 cut(s) | 171 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 292 |
| PdmI | GAANNNNTTC | 1 cut(s) | 325 |
| PflFI | GACNNNGTC | 1 cut(s) | 212 |
| PkrI | GCNGC | 2 cut(s) | 9, 410 |
| PleI | GAGTC | 2 cut(s) | 59, 291 |
| PpsI | GAGTC | 2 cut(s) | 59, 291 |
| PspPI | GGNCC | 1 cut(s) | 123 |
| PspXI | VCTCGAGB | 1 cut(s) | 280 |
| PsuI | RGATCY | 3 cut(s) | 73, 275, 451 |
| PsyI | GACNNNGTC | 1 cut(s) | 212 |
| RruI | TCGCGA | 1 cut(s) | 295 |
| RseI | CAYNNNNRTG | 1 cut(s) | 150 |
| SaqAI | TTAA | 1 cut(s) | 266 |
| SatI | GCNGC | 2 cut(s) | 8, 409 |
| Sau3AI | GATC | 4 cut(s) | 73, 252, 275, 451 |
| Sau96I | GGNCC | 1 cut(s) | 123 |
| SchI | GAGTC | 2 cut(s) | 60, 292 |
| ScrFI | CCNGG | 1 cut(s) | 115 |
| SetI | ASST | 7 cut(s) | 95, 125, 140, 213, 312, 366, 432 |
| SfaNI | GCATC | 2 cut(s) | 287, 346 |
| Sfr274I | CTCGAG | 1 cut(s) | 280 |
| SinI | GGWCC | 1 cut(s) | 123 |
| SlaI | CTCGAG | 1 cut(s) | 280 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 150 |
| SmlI | CTYRAG | 1 cut(s) | 280 |
| SmoI | CTYRAG | 1 cut(s) | 280 |
| SphI | GCATGC | 1 cut(s) | 442 |
| Sse9I | AATT | 1 cut(s) | 192 |
| SseBI | AGGCCT | 1 cut(s) | 171 |
| SsiI | CCGC | 3 cut(s) | 218, 398, 408 |
| SspMI | CTAG | 2 cut(s) | 11, 365 |
| StuI | AGGCCT | 1 cut(s) | 171 |
| StyD4I | CCNGG | 1 cut(s) | 113 |
| TaaI | ACNGT | 1 cut(s) | 244 |
| TaiI | ACGT | 1 cut(s) | 213 |
| TaqI | TCGA | 4 cut(s) | 142, 261, 281, 505 |
| TasI | AATT | 1 cut(s) | 192 |
| TauI | GCSGC | 1 cut(s) | 411 |
| Tru1I | TTAA | 1 cut(s) | 266 |
| Tru9I | TTAA | 1 cut(s) | 266 |
| TseI | GCWGC | 1 cut(s) | 7 |
| TspDTI | ATGAA | 4 cut(s) | 31, 68, 171, 486 |
| TspGWI | ACGGA | 1 cut(s) | 422 |
| Tth111I | GACNNNGTC | 1 cut(s) | 212 |
| VpaK11BI | GGWCC | 1 cut(s) | 123 |
| XceI | RCATGY | 1 cut(s) | 442 |
| XhoI | CTCGAG | 1 cut(s) | 280 |
| XmnI | GAANNNNTTC | 1 cut(s) | 325 |
| XspI | CTAG | 2 cut(s) | 11, 365 |