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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 239 |
| AccBSI | CCGCTC | 1 cut(s) | 190 |
| AciI | CCGC | 1 cut(s) | 190 |
| AclWI | GGATC | 1 cut(s) | 185 |
| AcoI | YGGCCR | 1 cut(s) | 18 |
| AcsI | RAATTY | 1 cut(s) | 259 |
| AcuI | CTGAAG | 1 cut(s) | 363 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 16, 251 |
| AgsI | TTSAA | 1 cut(s) | 259 |
| AluBI | AGCT | 5 cut(s) | 25, 70, 100, 290, 308 |
| AluI | AGCT | 5 cut(s) | 25, 70, 100, 290, 308 |
| Alw26I | GTCTC | 1 cut(s) | 198 |
| AlwI | GGATC | 1 cut(s) | 185 |
| Ama87I | CYCGRG | 2 cut(s) | 29, 93 |
| AoxI | GGCC | 2 cut(s) | 18, 265 |
| ApeKI | GCWGC | 2 cut(s) | 25, 100 |
| ApoI | RAATTY | 1 cut(s) | 259 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 351 |
| AsuC2I | CCSGG | 2 cut(s) | 94, 95 |
| AvaI | CYCGRG | 2 cut(s) | 29, 93 |
| BanII | GRGCYC | 1 cut(s) | 94 |
| BauI | CACGAG | 1 cut(s) | 356 |
| BbvI | GCAGC | 2 cut(s) | 12, 87 |
| BccI | CCATC | 1 cut(s) | 289 |
| BclI | TGATCA | 1 cut(s) | 157 |
| BcnI | CCSGG | 2 cut(s) | 94, 95 |
| BcoDI | GTCTC | 1 cut(s) | 198 |
| BfaI | CTAG | 4 cut(s) | 54, 71, 113, 197 |
| BisI | GCNGC | 2 cut(s) | 26, 101 |
| BlpI | GCTNAGC | 1 cut(s) | 66 |
| BlsI | GCNGC | 2 cut(s) | 27, 102 |
| Bme1390I | CCNGG | 2 cut(s) | 94, 95 |
| BmeT110I | CYCGRG | 2 cut(s) | 29, 93 |
| BmrFI | CCNGG | 2 cut(s) | 94, 95 |
| Bpu10I | CCTNAGC | 1 cut(s) | 59 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 66 |
| BpuMI | CCSGG | 2 cut(s) | 94, 95 |
| BsaI | GGTCTC | 1 cut(s) | 198 |
| BsaJI | CCNNGG | 1 cut(s) | 93 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 16, 251 |
| BseDI | CCNNGG | 1 cut(s) | 93 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 16, 251 |
| BseMII | CTCAG | 2 cut(s) | 57, 73 |
| BseRI | GAGGAG | 4 cut(s) | 152, 187, 221, 329 |
| BseX3I | CGGCCG | 1 cut(s) | 18 |
| BseXI | GCAGC | 2 cut(s) | 12, 87 |
| Bsh1285I | CGRYCG | 1 cut(s) | 21 |
| BshFI | GGCC | 2 cut(s) | 20, 267 |
| BsiEI | CGRYCG | 1 cut(s) | 21 |
| BsiHKCI | CYCGRG | 2 cut(s) | 29, 93 |
| BsiSI | CCGG | 1 cut(s) | 94 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 16, 251 |
| BsmAI | GTCTC | 1 cut(s) | 198 |
| BsmI | GAATGC | 1 cut(s) | 353 |
| BsnI | GGCC | 2 cut(s) | 20, 267 |
| Bso31I | GGTCTC | 1 cut(s) | 198 |
| BsoBI | CYCGRG | 2 cut(s) | 29, 93 |
| Bsp1286I | GDGCHC | 1 cut(s) | 94 |
| Bsp143I | GATC | 4 cut(s) | 115, 130, 157, 177 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 66 |
| BspACI | CCGC | 1 cut(s) | 190 |
| BspANI | GGCC | 2 cut(s) | 20, 267 |
| BspCNI | CTCAG | 2 cut(s) | 58, 72 |
| BspHI | TCATGA | 1 cut(s) | 118 |
| BspPI | GGATC | 1 cut(s) | 185 |
| BspQI | GCTCTTC | 1 cut(s) | 82 |
| BspTNI | GGTCTC | 1 cut(s) | 198 |
| BsrBI | CCGCTC | 1 cut(s) | 190 |
| BssECI | CCNNGG | 1 cut(s) | 93 |
| BssMI | GATC | 4 cut(s) | 115, 130, 157, 177 |
| BssSI | CACGAG | 1 cut(s) | 356 |
| Bst2BI | CACGAG | 1 cut(s) | 356 |
| Bst6I | CTCTTC | 1 cut(s) | 82 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 109 |
| BstC8I | GCNNGC | 1 cut(s) | 150 |
| BstDEI | CTNAG | 3 cut(s) | 59, 66, 291 |
| BstKTI | GATC | 4 cut(s) | 118, 133, 160, 180 |
| BstMAI | GTCTC | 1 cut(s) | 198 |
| BstMBI | GATC | 4 cut(s) | 115, 130, 157, 177 |
| BstMCI | CGRYCG | 1 cut(s) | 21 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 31, 109 |
| BstSCI | CCNGG | 2 cut(s) | 92, 93 |
| BstV1I | GCAGC | 2 cut(s) | 12, 87 |
| BstZI | CGGCCG | 1 cut(s) | 18 |
| BsuRI | GGCC | 2 cut(s) | 20, 267 |
| Cac8I | GCNNGC | 1 cut(s) | 150 |
| CciI | TCATGA | 1 cut(s) | 118 |
| Cfr9I | CCCGGG | 1 cut(s) | 93 |
| CviAII | CATG | 1 cut(s) | 119 |
| DdeI | CTNAG | 3 cut(s) | 59, 66, 291 |
| DpnI | GATC | 4 cut(s) | 117, 132, 159, 179 |
| DpnII | GATC | 4 cut(s) | 115, 130, 157, 177 |
| EaeI | YGGCCR | 1 cut(s) | 18 |
| EagI | CGGCCG | 1 cut(s) | 18 |
| Eam1104I | CTCTTC | 1 cut(s) | 82 |
| EarI | CTCTTC | 1 cut(s) | 82 |
| EclXI | CGGCCG | 1 cut(s) | 18 |
| Eco147I | AGGCCT | 1 cut(s) | 267 |
| Eco24I | GRGCYC | 1 cut(s) | 94 |
| Eco31I | GGTCTC | 1 cut(s) | 198 |
| Eco52I | CGGCCG | 1 cut(s) | 18 |
| Eco57I | CTGAAG | 1 cut(s) | 363 |
| Eco88I | CYCGRG | 2 cut(s) | 29, 93 |
| EcoT38I | GRGCYC | 1 cut(s) | 94 |
| FaeI | CATG | 1 cut(s) | 122 |
| FaiI | YATR | 2 cut(s) | 120, 239 |
| FatI | CATG | 1 cut(s) | 118 |
| FbaI | TGATCA | 1 cut(s) | 157 |
| Fnu4HI | GCNGC | 2 cut(s) | 26, 101 |
| FriOI | GRGCYC | 1 cut(s) | 94 |
| Fsp4HI | GCNGC | 2 cut(s) | 26, 101 |
| FspBI | CTAG | 4 cut(s) | 54, 71, 113, 197 |
| GluI | GCNGC | 2 cut(s) | 26, 101 |
| HaeIII | GGCC | 2 cut(s) | 20, 267 |
| HapII | CCGG | 1 cut(s) | 94 |
| Hin1II | CATG | 1 cut(s) | 122 |
| HindIII | AAGCTT | 1 cut(s) | 306 |
| HinfI | GANTC | 1 cut(s) | 14 |
| HpaII | CCGG | 1 cut(s) | 94 |
| Hpy188III | TCNNGA | 3 cut(s) | 119, 134, 356 |
| HpyAV | CCTTC | 1 cut(s) | 270 |
| HpyCH4V | TGCA | 3 cut(s) | 103, 283, 328 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 31, 109 |
| HpyF3I | CTNAG | 3 cut(s) | 59, 66, 291 |
| Hsp92II | CATG | 1 cut(s) | 122 |
| Ksp22I | TGATCA | 1 cut(s) | 157 |
| Kzo9I | GATC | 4 cut(s) | 115, 130, 157, 177 |
| LguI | GCTCTTC | 1 cut(s) | 82 |
| LmnI | GCTCC | 2 cut(s) | 97, 187 |
| LpnPI | CCDG | 4 cut(s) | 46, 107, 134, 146 |
| Lsp1109I | GCAGC | 2 cut(s) | 12, 87 |
| MaeI | CTAG | 4 cut(s) | 54, 71, 113, 197 |
| MalI | GATC | 4 cut(s) | 117, 132, 159, 179 |
| MbiI | CCGCTC | 1 cut(s) | 190 |
| MboI | GATC | 4 cut(s) | 115, 130, 157, 177 |
| MboII | GAAGA | 2 cut(s) | 99, 356 |
| MfeI | CAATTG | 1 cut(s) | 153 |
| MhlI | GDGCHC | 1 cut(s) | 94 |
| MluCI | AATT | 3 cut(s) | 153, 259, 329 |
| MlyI | GAGTC | 1 cut(s) | 23 |
| MmeI | TCCRAC | 2 cut(s) | 165, 342 |
| MroXI | GAANNNNTTC | 1 cut(s) | 351 |
| MseI | TTAA | 1 cut(s) | 302 |
| MspI | CCGG | 1 cut(s) | 94 |
| MspR9I | CCNGG | 2 cut(s) | 94, 95 |
| MunI | CAATTG | 1 cut(s) | 153 |
| Mva1269I | GAATGC | 1 cut(s) | 353 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 31, 109 |
| NciI | CCSGG | 2 cut(s) | 94, 95 |
| NdeII | GATC | 4 cut(s) | 115, 130, 157, 177 |
| NlaIII | CATG | 1 cut(s) | 122 |
| NmeAIII | GCCGAG | 1 cut(s) | 46 |
| PaeR7I | CTCGAG | 1 cut(s) | 29 |
| PagI | TCATGA | 1 cut(s) | 118 |
| PceI | AGGCCT | 1 cut(s) | 267 |
| PciSI | GCTCTTC | 1 cut(s) | 82 |
| PctI | GAATGC | 1 cut(s) | 353 |
| PdmI | GAANNNNTTC | 1 cut(s) | 351 |
| PkrI | GCNGC | 2 cut(s) | 27, 102 |
| PleI | GAGTC | 1 cut(s) | 22 |
| PpsI | GAGTC | 1 cut(s) | 22 |
| PsiI | TTATAA | 1 cut(s) | 239 |
| PspXI | VCTCGAGB | 1 cut(s) | 29 |
| SapI | GCTCTTC | 1 cut(s) | 82 |
| SaqAI | TTAA | 1 cut(s) | 302 |
| SatI | GCNGC | 2 cut(s) | 26, 101 |
| Sau3AI | GATC | 4 cut(s) | 115, 130, 157, 177 |
| SchI | GAGTC | 1 cut(s) | 23 |
| ScrFI | CCNGG | 2 cut(s) | 94, 95 |
| SduI | GDGCHC | 1 cut(s) | 94 |
| SetI | ASST | 9 cut(s) | 27, 72, 102, 129, 237, 281, 292, 310, 321 |
| Sfr274I | CTCGAG | 1 cut(s) | 29 |
| SlaI | CTCGAG | 1 cut(s) | 29 |
| SmaI | CCCGGG | 1 cut(s) | 95 |
| SmlI | CTYRAG | 1 cut(s) | 29 |
| SmoI | CTYRAG | 1 cut(s) | 29 |
| Sse9I | AATT | 3 cut(s) | 153, 259, 329 |
| SseBI | AGGCCT | 1 cut(s) | 267 |
| SsiI | CCGC | 1 cut(s) | 190 |
| SspMI | CTAG | 4 cut(s) | 54, 71, 113, 197 |
| StuI | AGGCCT | 1 cut(s) | 267 |
| StyD4I | CCNGG | 2 cut(s) | 92, 93 |
| TaqI | TCGA | 2 cut(s) | 30, 180 |
| TasI | AATT | 3 cut(s) | 153, 259, 329 |
| Tru1I | TTAA | 1 cut(s) | 302 |
| Tru9I | TTAA | 1 cut(s) | 302 |
| TseI | GCWGC | 2 cut(s) | 25, 100 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspGWI | ACGGA | 1 cut(s) | 288 |
| TspMI | CCCGGG | 1 cut(s) | 93 |
| XapI | RAATTY | 1 cut(s) | 259 |
| XhoI | CTCGAG | 1 cut(s) | 29 |
| XmaI | CCCGGG | 1 cut(s) | 93 |
| XmnI | GAANNNNTTC | 1 cut(s) | 351 |
| XspI | CTAG | 4 cut(s) | 54, 71, 113, 197 |