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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 66 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 16 |
| AccI | GTMKAC | 1 cut(s) | 70 |
| AciI | CCGC | 2 cut(s) | 183, 266 |
| AclWI | GGATC | 1 cut(s) | 582 |
| AcsI | RAATTY | 1 cut(s) | 285 |
| AcyI | GRCGYC | 1 cut(s) | 63 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 16, 196, 211 |
| AflII | CTTAAG | 1 cut(s) | 602 |
| AgsI | TTSAA | 1 cut(s) | 641 |
| AjnI | CCWGG | 1 cut(s) | 344 |
| AluBI | AGCT | 5 cut(s) | 451, 461, 542, 586, 658 |
| AluI | AGCT | 5 cut(s) | 451, 461, 542, 586, 658 |
| Alw26I | GTCTC | 4 cut(s) | 41, 173, 410, 592 |
| AlwI | GGATC | 1 cut(s) | 582 |
| Ama87I | CYCGRG | 1 cut(s) | 206 |
| AoxI | GGCC | 1 cut(s) | 342 |
| ApeKI | GCWGC | 1 cut(s) | 655 |
| ApoI | RAATTY | 1 cut(s) | 285 |
| AspLEI | GCGC | 2 cut(s) | 43, 247 |
| AsuHPI | GGTGA | 1 cut(s) | 92 |
| AvaI | CYCGRG | 1 cut(s) | 206 |
| BbvCI | CCTCAGC | 1 cut(s) | 248 |
| BbvI | GCAGC | 1 cut(s) | 667 |
| BccI | CCATC | 1 cut(s) | 92 |
| BciT130I | CCWGG | 1 cut(s) | 346 |
| BcoDI | GTCTC | 4 cut(s) | 41, 173, 410, 592 |
| BfrI | CTTAAG | 1 cut(s) | 602 |
| BisI | GCNGC | 1 cut(s) | 656 |
| BlpI | GCTNAGC | 1 cut(s) | 582 |
| BlsI | GCNGC | 1 cut(s) | 657 |
| Bme1390I | CCNGG | 1 cut(s) | 346 |
| BmeT110I | CYCGRG | 1 cut(s) | 206 |
| BmrFI | CCNGG | 1 cut(s) | 346 |
| BmsI | GCATC | 3 cut(s) | 28, 361, 457 |
| BoxI | GACNNNNGTC | 1 cut(s) | 66 |
| BpmI | CTGGAG | 1 cut(s) | 65 |
| Bpu10I | CCTNAGC | 1 cut(s) | 248 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 582 |
| BsaBI | GATNNNNATC | 1 cut(s) | 573 |
| BsaHI | GRCGYC | 1 cut(s) | 63 |
| BsaI | GGTCTC | 2 cut(s) | 41, 173 |
| BsaJI | CCNNGG | 1 cut(s) | 205 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 16, 196, 211 |
| Bse1I | ACTGG | 1 cut(s) | 315 |
| Bse8I | GATNNNNATC | 1 cut(s) | 573 |
| BseBI | CCWGG | 1 cut(s) | 346 |
| BseDI | CCNNGG | 1 cut(s) | 205 |
| BseGI | GGATG | 3 cut(s) | 205, 298, 376 |
| BseJI | GATNNNNATC | 1 cut(s) | 573 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 16, 196, 211 |
| BseMII | CTCAG | 4 cut(s) | 222, 239, 317, 573 |
| BseNI | ACTGG | 1 cut(s) | 315 |
| BseRI | GAGGAG | 1 cut(s) | 665 |
| BseXI | GCAGC | 1 cut(s) | 667 |
| BshFI | GGCC | 1 cut(s) | 344 |
| BsiHKCI | CYCGRG | 1 cut(s) | 206 |
| BslFI | GGGAC | 1 cut(s) | 228 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 16, 196, 211 |
| BsmAI | GTCTC | 4 cut(s) | 41, 173, 410, 592 |
| BsmFI | GGGAC | 1 cut(s) | 228 |
| BsnI | GGCC | 1 cut(s) | 344 |
| Bso31I | GGTCTC | 2 cut(s) | 41, 173 |
| BsoBI | CYCGRG | 1 cut(s) | 206 |
| Bsp143I | GATC | 2 cut(s) | 109, 574 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 582 |
| BspACI | CCGC | 2 cut(s) | 183, 266 |
| BspANI | GGCC | 1 cut(s) | 344 |
| BspCNI | CTCAG | 4 cut(s) | 223, 240, 318, 574 |
| BspPI | GGATC | 1 cut(s) | 582 |
| BspTI | CTTAAG | 1 cut(s) | 602 |
| BspTNI | GGTCTC | 2 cut(s) | 41, 173 |
| BsrI | ACTGG | 1 cut(s) | 315 |
| BssECI | CCNNGG | 1 cut(s) | 205 |
| BssMI | GATC | 2 cut(s) | 109, 574 |
| BssNI | GRCGYC | 1 cut(s) | 63 |
| Bst2UI | CCWGG | 1 cut(s) | 346 |
| Bst4CI | ACNGT | 2 cut(s) | 398, 594 |
| Bst6I | CTCTTC | 3 cut(s) | 171, 255, 513 |
| BstACI | GRCGYC | 1 cut(s) | 63 |
| BstAFI | CTTAAG | 1 cut(s) | 602 |
| BstC8I | GCNNGC | 2 cut(s) | 360, 459 |
| BstDEI | CTNAG | 7 cut(s) | 231, 248, 326, 363, 375, 500, 582 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 209 |
| BstF5I | GGATG | 3 cut(s) | 205, 298, 376 |
| BstHHI | GCGC | 2 cut(s) | 43, 247 |
| BstKTI | GATC | 2 cut(s) | 112, 577 |
| BstMAI | GTCTC | 4 cut(s) | 41, 173, 410, 592 |
| BstMBI | GATC | 2 cut(s) | 109, 574 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 38, 467 |
| BstNI | CCWGG | 1 cut(s) | 346 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 66 |
| BstSCI | CCNGG | 1 cut(s) | 344 |
| BstV1I | GCAGC | 1 cut(s) | 667 |
| BstX2I | RGATCY | 1 cut(s) | 574 |
| BstYI | RGATCY | 1 cut(s) | 574 |
| BsuRI | GGCC | 1 cut(s) | 344 |
| BtsCI | GGATG | 3 cut(s) | 205, 298, 376 |
| BtsIMutI | CAGTG | 1 cut(s) | 590 |
| Cac8I | GCNNGC | 2 cut(s) | 360, 459 |
| CfoI | GCGC | 2 cut(s) | 43, 247 |
| CviAII | CATG | 4 cut(s) | 11, 59, 172, 301 |
| CviJI | RGCY | 9 cut(s) | 332, 344, 362, 451, 461, 504, 542, 586, 658 |
| CviKI_1 | RGCY | 9 cut(s) | 332, 344, 362, 451, 461, 504, 542, 586, 658 |
| DdeI | CTNAG | 7 cut(s) | 231, 248, 326, 363, 375, 500, 582 |
| DpnI | GATC | 2 cut(s) | 111, 576 |
| DpnII | GATC | 2 cut(s) | 109, 574 |
| DraI | TTTAAA | 1 cut(s) | 423 |
| Eam1104I | CTCTTC | 3 cut(s) | 171, 255, 513 |
| EarI | CTCTTC | 3 cut(s) | 171, 255, 513 |
| Eco31I | GGTCTC | 2 cut(s) | 41, 173 |
| Eco88I | CYCGRG | 1 cut(s) | 206 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 209 |
| EcoRI | GAATTC | 1 cut(s) | 285 |
| EcoRII | CCWGG | 1 cut(s) | 344 |
| FaeI | CATG | 4 cut(s) | 14, 62, 175, 304 |
| FaqI | GGGAC | 1 cut(s) | 228 |
| FatI | CATG | 4 cut(s) | 10, 58, 171, 300 |
| FauI | CCCGC | 1 cut(s) | 259 |
| FblI | GTMKAC | 1 cut(s) | 70 |
| Fnu4HI | GCNGC | 1 cut(s) | 656 |
| FokI | GGATG | 3 cut(s) | 212, 305, 383 |
| Fsp4HI | GCNGC | 1 cut(s) | 656 |
| GlaI | GCGC | 2 cut(s) | 42, 246 |
| GluI | GCNGC | 1 cut(s) | 656 |
| GsuI | CTGGAG | 1 cut(s) | 65 |
| HaeIII | GGCC | 1 cut(s) | 344 |
| HhaI | GCGC | 2 cut(s) | 43, 247 |
| Hin1I | GRCGYC | 1 cut(s) | 63 |
| Hin1II | CATG | 4 cut(s) | 14, 62, 175, 304 |
| Hin6I | GCGC | 2 cut(s) | 41, 245 |
| HinP1I | GCGC | 2 cut(s) | 41, 245 |
| HincII | GTYRAC | 1 cut(s) | 169 |
| HindII | GTYRAC | 1 cut(s) | 169 |
| HinfI | GANTC | 3 cut(s) | 27, 126, 401 |
| HphI | GGTGA | 1 cut(s) | 92 |
| Hpy166II | GTNNAC | 2 cut(s) | 71, 169 |
| Hpy188I | TCNGA | 1 cut(s) | 635 |
| Hpy188III | TCNNGA | 2 cut(s) | 95, 290 |
| Hpy8I | GTNNAC | 2 cut(s) | 71, 169 |
| HpyCH4III | ACNGT | 2 cut(s) | 398, 594 |
| HpyCH4IV | ACGT | 1 cut(s) | 63 |
| HpyCH4V | TGCA | 2 cut(s) | 358, 412 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 38, 467 |
| HpyF3I | CTNAG | 7 cut(s) | 231, 248, 326, 363, 375, 500, 582 |
| HpySE526I | ACGT | 1 cut(s) | 63 |
| Hsp92I | GRCGYC | 1 cut(s) | 63 |
| Hsp92II | CATG | 4 cut(s) | 14, 62, 175, 304 |
| HspAI | GCGC | 2 cut(s) | 41, 245 |
| Kzo9I | GATC | 2 cut(s) | 109, 574 |
| LmnI | GCTCC | 2 cut(s) | 526, 652 |
| Lsp1109I | GCAGC | 1 cut(s) | 667 |
| LweI | GCATC | 3 cut(s) | 28, 361, 457 |
| MaeII | ACGT | 1 cut(s) | 63 |
| MaeIII | GTNAC | 1 cut(s) | 80 |
| MalI | GATC | 2 cut(s) | 111, 576 |
| MboI | GATC | 2 cut(s) | 109, 574 |
| MboII | GAAGA | 6 cut(s) | 161, 188, 272, 530, 544, 673 |
| MflI | RGATCY | 1 cut(s) | 574 |
| MluCI | AATT | 4 cut(s) | 14, 285, 304, 353 |
| MlyI | GAGTC | 1 cut(s) | 21 |
| MmeI | TCCRAC | 4 cut(s) | 100, 348, 504, 658 |
| MseI | TTAA | 3 cut(s) | 339, 422, 603 |
| MspA1I | CMGCKG | 1 cut(s) | 658 |
| MspCI | CTTAAG | 1 cut(s) | 602 |
| MspR9I | CCNGG | 1 cut(s) | 346 |
| MvaI | CCWGG | 1 cut(s) | 346 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 38, 467 |
| NdeII | GATC | 2 cut(s) | 109, 574 |
| NlaIII | CATG | 4 cut(s) | 14, 62, 175, 304 |
| NmuCI | GTSAC | 1 cut(s) | 80 |
| PfeI | GAWTC | 2 cut(s) | 126, 401 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 16 |
| PkrI | GCNGC | 1 cut(s) | 657 |
| PleI | GAGTC | 1 cut(s) | 21 |
| PpsI | GAGTC | 1 cut(s) | 21 |
| PshAI | GACNNNNGTC | 1 cut(s) | 66 |
| Psp6I | CCWGG | 1 cut(s) | 344 |
| PspGI | CCWGG | 1 cut(s) | 344 |
| PsuI | RGATCY | 1 cut(s) | 574 |
| PvuII | CAGCTG | 1 cut(s) | 658 |
| SaqAI | TTAA | 3 cut(s) | 339, 422, 603 |
| SatI | GCNGC | 1 cut(s) | 656 |
| Sau3AI | GATC | 2 cut(s) | 109, 574 |
| SchI | GAGTC | 1 cut(s) | 21 |
| ScrFI | CCNGG | 1 cut(s) | 346 |
| SfaNI | GCATC | 3 cut(s) | 28, 361, 457 |
| SmlI | CTYRAG | 1 cut(s) | 602 |
| SmoI | CTYRAG | 1 cut(s) | 602 |
| Sse9I | AATT | 4 cut(s) | 14, 285, 304, 353 |
| SsiI | CCGC | 2 cut(s) | 183, 266 |
| StyD4I | CCNGG | 1 cut(s) | 344 |
| TaaI | ACNGT | 2 cut(s) | 398, 594 |
| TaiI | ACGT | 1 cut(s) | 66 |
| TaqI | TCGA | 1 cut(s) | 335 |
| TasI | AATT | 4 cut(s) | 14, 285, 304, 353 |
| TfiI | GAWTC | 2 cut(s) | 126, 401 |
| Tru1I | TTAA | 3 cut(s) | 339, 422, 603 |
| Tru9I | TTAA | 3 cut(s) | 339, 422, 603 |
| TscAI | CASTG | 1 cut(s) | 597 |
| TseFI | GTSAC | 1 cut(s) | 80 |
| TseI | GCWGC | 1 cut(s) | 655 |
| Tsp45I | GTSAC | 1 cut(s) | 80 |
| TspDTI | ATGAA | 3 cut(s) | 27, 317, 552 |
| TspGWI | ACGGA | 2 cut(s) | 104, 433 |
| TspRI | CASTG | 1 cut(s) | 597 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 16 |
| Vha464I | CTTAAG | 1 cut(s) | 602 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 209 |
| XapI | RAATTY | 1 cut(s) | 285 |
| XmiI | GTMKAC | 1 cut(s) | 70 |
| ZraI | GACGTC | 1 cut(s) | 64 |