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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 319, 449 |
| AccI | GTMKAC | 1 cut(s) | 131 |
| AciI | CCGC | 7 cut(s) | 135, 245, 277, 313, 316, 566, 698 |
| AcyI | GRCGYC | 1 cut(s) | 90 |
| AfaI | GTAC | 2 cut(s) | 232, 256 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 100, 283, 396, 554 |
| AgeI | ACCGGT | 1 cut(s) | 389 |
| AgsI | TTSAA | 3 cut(s) | 362, 413, 654 |
| AjiI | CACGTC | 1 cut(s) | 118 |
| AjnI | CCWGG | 1 cut(s) | 93 |
| AluBI | AGCT | 1 cut(s) | 594 |
| AluI | AGCT | 1 cut(s) | 594 |
| Alw21I | GWGCWC | 1 cut(s) | 565 |
| Alw26I | GTCTC | 1 cut(s) | 571 |
| Ama87I | CYCGRG | 2 cut(s) | 548, 574 |
| AoxI | GGCC | 3 cut(s) | 463, 571, 627 |
| AsiGI | ACCGGT | 1 cut(s) | 389 |
| AspLEI | GCGC | 2 cut(s) | 375, 697 |
| AspS9I | GGNCC | 4 cut(s) | 247, 463, 572, 634 |
| AsuC2I | CCSGG | 3 cut(s) | 252, 407, 467 |
| AsuHPI | GGTGA | 2 cut(s) | 109, 464 |
| AvaI | CYCGRG | 2 cut(s) | 548, 574 |
| AvaII | GGWCC | 2 cut(s) | 247, 634 |
| BanI | GGYRCC | 2 cut(s) | 319, 449 |
| BbsI | GAAGAC | 1 cut(s) | 420 |
| Bbv12I | GWGCWC | 1 cut(s) | 565 |
| BccI | CCATC | 4 cut(s) | 184, 532, 608, 683 |
| BceAI | ACGGC | 1 cut(s) | 614 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 149, 183, 374, 408 |
| BciT130I | CCWGG | 1 cut(s) | 95 |
| BcnI | CCSGG | 3 cut(s) | 252, 407, 467 |
| BcoDI | GTCTC | 1 cut(s) | 571 |
| BfaI | CTAG | 2 cut(s) | 396, 425 |
| BfoI | RGCGCY | 2 cut(s) | 376, 698 |
| BisI | GCNGC | 2 cut(s) | 314, 698 |
| BlsI | GCNGC | 2 cut(s) | 315, 699 |
| Bme1390I | CCNGG | 4 cut(s) | 95, 252, 407, 467 |
| Bme18I | GGWCC | 2 cut(s) | 247, 634 |
| BmeT110I | CYCGRG | 2 cut(s) | 548, 574 |
| BmgBI | CACGTC | 1 cut(s) | 118 |
| BmgT120I | GGNCC | 4 cut(s) | 247, 463, 572, 634 |
| BmiI | GGNNCC | 3 cut(s) | 249, 321, 451 |
| BmrFI | CCNGG | 4 cut(s) | 95, 252, 407, 467 |
| BmsI | GCATC | 2 cut(s) | 136, 162 |
| BpiI | GAAGAC | 1 cut(s) | 420 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 133, 165 |
| BpuMI | CCSGG | 3 cut(s) | 252, 407, 467 |
| BsaHI | GRCGYC | 1 cut(s) | 90 |
| BsaJI | CCNNGG | 4 cut(s) | 250, 466, 549, 630 |
| BsaWI | WCCGGW | 2 cut(s) | 378, 389 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 100, 283, 396, 554 |
| Bse118I | RCCGGY | 1 cut(s) | 389 |
| BseBI | CCWGG | 1 cut(s) | 95 |
| BseDI | CCNNGG | 4 cut(s) | 250, 466, 549, 630 |
| BseGI | GGATG | 3 cut(s) | 535, 619, 694 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 100, 283, 396, 554 |
| BseRI | GAGGAG | 2 cut(s) | 199, 365 |
| BshFI | GGCC | 3 cut(s) | 465, 573, 629 |
| BshNI | GGYRCC | 2 cut(s) | 319, 449 |
| BshTI | ACCGGT | 1 cut(s) | 389 |
| BsiHKAI | GWGCWC | 1 cut(s) | 565 |
| BsiHKCI | CYCGRG | 2 cut(s) | 548, 574 |
| BsiSI | CCGG | 5 cut(s) | 252, 379, 390, 407, 466 |
| BslFI | GGGAC | 1 cut(s) | 233 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 100, 283, 396, 554 |
| BsmAI | GTCTC | 1 cut(s) | 571 |
| BsmFI | GGGAC | 1 cut(s) | 233 |
| BsnI | GGCC | 3 cut(s) | 465, 573, 629 |
| BsoBI | CYCGRG | 2 cut(s) | 548, 574 |
| Bsp1286I | GDGCHC | 1 cut(s) | 565 |
| Bsp1407I | TGTACA | 1 cut(s) | 230 |
| Bsp143I | GATC | 1 cut(s) | 339 |
| BspACI | CCGC | 7 cut(s) | 135, 245, 277, 313, 316, 566, 698 |
| BspANI | GGCC | 3 cut(s) | 465, 573, 629 |
| BspLI | GGNNCC | 3 cut(s) | 249, 321, 451 |
| BspT107I | GGYRCC | 2 cut(s) | 319, 449 |
| BsrFI | RCCGGY | 1 cut(s) | 389 |
| BsrGI | TGTACA | 1 cut(s) | 230 |
| BssAI | RCCGGY | 1 cut(s) | 389 |
| BssECI | CCNNGG | 4 cut(s) | 250, 466, 549, 630 |
| BssMI | GATC | 1 cut(s) | 339 |
| BssNI | GRCGYC | 1 cut(s) | 90 |
| Bst2UI | CCWGG | 1 cut(s) | 95 |
| Bst6I | CTCTTC | 1 cut(s) | 26 |
| BstACI | GRCGYC | 1 cut(s) | 90 |
| BstAUI | TGTACA | 1 cut(s) | 230 |
| BstC8I | GCNNGC | 1 cut(s) | 223 |
| BstDSI | CCRYGG | 1 cut(s) | 630 |
| BstEII | GGTNACC | 1 cut(s) | 470 |
| BstF5I | GGATG | 3 cut(s) | 535, 619, 694 |
| BstH2I | RGCGCY | 2 cut(s) | 376, 698 |
| BstHHI | GCGC | 2 cut(s) | 375, 697 |
| BstKTI | GATC | 1 cut(s) | 342 |
| BstMAI | GTCTC | 1 cut(s) | 571 |
| BstMBI | GATC | 1 cut(s) | 339 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 319, 381, 615 |
| BstNI | CCWGG | 1 cut(s) | 95 |
| BstPI | GGTNACC | 1 cut(s) | 470 |
| BstSCI | CCNGG | 4 cut(s) | 93, 250, 405, 465 |
| BstV2I | GAAGAC | 1 cut(s) | 420 |
| BsuRI | GGCC | 3 cut(s) | 465, 573, 629 |
| BtgI | CCRYGG | 1 cut(s) | 630 |
| BtgZI | GCGATG | 1 cut(s) | 185 |
| BtrI | CACGTC | 1 cut(s) | 118 |
| BtsCI | GGATG | 3 cut(s) | 535, 619, 694 |
| Cac8I | GCNNGC | 1 cut(s) | 223 |
| CfoI | GCGC | 2 cut(s) | 375, 697 |
| Cfr10I | RCCGGY | 1 cut(s) | 389 |
| Cfr13I | GGNCC | 4 cut(s) | 247, 463, 572, 634 |
| CseI | GACGC | 1 cut(s) | 79 |
| Csp6I | GTAC | 2 cut(s) | 231, 255 |
| CspAI | ACCGGT | 1 cut(s) | 389 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 612, 647 |
| CviJI | RGCY | 6 cut(s) | 465, 573, 594, 604, 629, 680 |
| CviKI_1 | RGCY | 6 cut(s) | 465, 573, 594, 604, 629, 680 |
| CviQI | GTAC | 2 cut(s) | 231, 255 |
| DpnI | GATC | 1 cut(s) | 341 |
| DpnII | GATC | 1 cut(s) | 339 |
| Eam1104I | CTCTTC | 1 cut(s) | 26 |
| EarI | CTCTTC | 1 cut(s) | 26 |
| Eco47I | GGWCC | 2 cut(s) | 247, 634 |
| Eco88I | CYCGRG | 2 cut(s) | 548, 574 |
| Eco91I | GGTNACC | 1 cut(s) | 470 |
| EcoO65I | GGTNACC | 1 cut(s) | 470 |
| EcoRII | CCWGG | 1 cut(s) | 93 |
| FaiI | YATR | 5 cut(s) | 52, 156, 219, 284, 599 |
| FaqI | GGGAC | 1 cut(s) | 233 |
| FauI | CCCGC | 1 cut(s) | 309 |
| FblI | GTMKAC | 1 cut(s) | 131 |
| Fnu4HI | GCNGC | 2 cut(s) | 314, 698 |
| FokI | GGATG | 3 cut(s) | 542, 626, 701 |
| Fsp4HI | GCNGC | 2 cut(s) | 314, 698 |
| FspBI | CTAG | 2 cut(s) | 396, 425 |
| GlaI | GCGC | 2 cut(s) | 374, 696 |
| GluI | GCNGC | 2 cut(s) | 314, 698 |
| HaeII | RGCGCY | 2 cut(s) | 376, 698 |
| HaeIII | GGCC | 3 cut(s) | 465, 573, 629 |
| HapII | CCGG | 5 cut(s) | 252, 379, 390, 407, 466 |
| HgaI | GACGC | 1 cut(s) | 79 |
| HhaI | GCGC | 2 cut(s) | 375, 697 |
| Hin1I | GRCGYC | 1 cut(s) | 90 |
| Hin6I | GCGC | 2 cut(s) | 373, 695 |
| HinP1I | GCGC | 2 cut(s) | 373, 695 |
| HinfI | GANTC | 3 cut(s) | 128, 199, 517 |
| HpaII | CCGG | 5 cut(s) | 252, 379, 390, 407, 466 |
| HphI | GGTGA | 2 cut(s) | 109, 464 |
| Hpy166II | GTNNAC | 3 cut(s) | 21, 132, 634 |
| Hpy188I | TCNGA | 2 cut(s) | 204, 688 |
| Hpy188III | TCNNGA | 3 cut(s) | 22, 500, 548 |
| Hpy8I | GTNNAC | 3 cut(s) | 21, 132, 634 |
| Hpy99I | CGWCG | 2 cut(s) | 119, 651 |
| HpyAV | CCTTC | 2 cut(s) | 19, 691 |
| HpyCH4IV | ACGT | 3 cut(s) | 117, 417, 543 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 319, 381, 615 |
| HpySE526I | ACGT | 3 cut(s) | 117, 417, 543 |
| Hsp92I | GRCGYC | 1 cut(s) | 90 |
| HspAI | GCGC | 2 cut(s) | 373, 695 |
| Kzo9I | GATC | 1 cut(s) | 339 |
| LmnI | GCTCC | 6 cut(s) | 154, 370, 381, 568, 599, 692 |
| LpnPI | CCDG | 8 cut(s) | 80, 107, 235, 265, 392, 403, 420, 479 |
| LweI | GCATC | 2 cut(s) | 136, 162 |
| MaeI | CTAG | 2 cut(s) | 396, 425 |
| MaeII | ACGT | 3 cut(s) | 117, 417, 543 |
| MaeIII | GTNAC | 3 cut(s) | 188, 470, 511 |
| MalI | GATC | 1 cut(s) | 341 |
| MboI | GATC | 1 cut(s) | 339 |
| MboII | GAAGA | 5 cut(s) | 43, 374, 425, 635, 679 |
| MhlI | GDGCHC | 1 cut(s) | 565 |
| MluCI | AATT | 1 cut(s) | 654 |
| MlyI | GAGTC | 3 cut(s) | 137, 193, 526 |
| MseI | TTAA | 1 cut(s) | 660 |
| MslI | CAYNNNNRTG | 1 cut(s) | 116 |
| MspI | CCGG | 5 cut(s) | 252, 379, 390, 407, 466 |
| MspR9I | CCNGG | 4 cut(s) | 95, 252, 407, 467 |
| MvaI | CCWGG | 1 cut(s) | 95 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 319, 381, 615 |
| NciI | CCSGG | 3 cut(s) | 252, 407, 467 |
| NdeII | GATC | 1 cut(s) | 339 |
| NlaIV | GGNNCC | 3 cut(s) | 249, 321, 451 |
| NmuCI | GTSAC | 2 cut(s) | 470, 511 |
| PinAI | ACCGGT | 1 cut(s) | 389 |
| PkrI | GCNGC | 2 cut(s) | 315, 699 |
| PleI | GAGTC | 3 cut(s) | 136, 193, 525 |
| PpsI | GAGTC | 3 cut(s) | 136, 193, 525 |
| Psp6I | CCWGG | 1 cut(s) | 93 |
| PspEI | GGTNACC | 1 cut(s) | 470 |
| PspGI | CCWGG | 1 cut(s) | 93 |
| PspN4I | GGNNCC | 3 cut(s) | 249, 321, 451 |
| PspPI | GGNCC | 4 cut(s) | 247, 463, 572, 634 |
| RsaI | GTAC | 2 cut(s) | 232, 256 |
| RsaNI | GTAC | 2 cut(s) | 231, 255 |
| RseI | CAYNNNNRTG | 1 cut(s) | 116 |
| SaqAI | TTAA | 1 cut(s) | 660 |
| SatI | GCNGC | 2 cut(s) | 314, 698 |
| Sau3AI | GATC | 1 cut(s) | 339 |
| Sau96I | GGNCC | 4 cut(s) | 247, 463, 572, 634 |
| SchI | GAGTC | 3 cut(s) | 137, 193, 526 |
| ScrFI | CCNGG | 4 cut(s) | 95, 252, 407, 467 |
| SduI | GDGCHC | 1 cut(s) | 565 |
| SetI | ASST | 9 cut(s) | 120, 209, 328, 420, 483, 507, 546, 555, 596 |
| SfaNI | GCATC | 2 cut(s) | 136, 162 |
| SinI | GGWCC | 2 cut(s) | 247, 634 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 116 |
| Sse9I | AATT | 1 cut(s) | 654 |
| SsiI | CCGC | 7 cut(s) | 135, 245, 277, 313, 316, 566, 698 |
| SspMI | CTAG | 2 cut(s) | 396, 425 |
| StyD4I | CCNGG | 4 cut(s) | 93, 250, 405, 465 |
| TaiI | ACGT | 3 cut(s) | 120, 420, 546 |
| TaqI | TCGA | 3 cut(s) | 114, 238, 536 |
| TasI | AATT | 1 cut(s) | 654 |
| TatI | WGTACW | 1 cut(s) | 230 |
| TauI | GCSGC | 2 cut(s) | 316, 700 |
| Tru1I | TTAA | 1 cut(s) | 660 |
| Tru9I | TTAA | 1 cut(s) | 660 |
| TseFI | GTSAC | 2 cut(s) | 470, 511 |
| Tsp45I | GTSAC | 2 cut(s) | 470, 511 |
| TspDTI | ATGAA | 2 cut(s) | 100, 686 |
| TspGWI | ACGGA | 1 cut(s) | 334 |
| VpaK11BI | GGWCC | 2 cut(s) | 247, 634 |
| XmiI | GTMKAC | 1 cut(s) | 131 |
| XspI | CTAG | 2 cut(s) | 396, 425 |