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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 255 |
| AccBSI | CCGCTC | 1 cut(s) | 101 |
| AccI | GTMKAC | 1 cut(s) | 306 |
| AccII | CGCG | 1 cut(s) | 73 |
| AccIII | TCCGGA | 2 cut(s) | 28, 396 |
| AciI | CCGC | 5 cut(s) | 13, 16, 73, 101, 455 |
| AclWI | GGATC | 1 cut(s) | 239 |
| AcyI | GRCGYC | 1 cut(s) | 252 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 72, 319, 339, 461 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 440 |
| AluBI | AGCT | 1 cut(s) | 107 |
| AluI | AGCT | 1 cut(s) | 107 |
| Alw26I | GTCTC | 2 cut(s) | 207, 398 |
| AlwI | GGATC | 1 cut(s) | 239 |
| Ama87I | CYCGRG | 3 cut(s) | 212, 461, 474 |
| Aor13HI | TCCGGA | 2 cut(s) | 28, 396 |
| AoxI | GGCC | 2 cut(s) | 208, 428 |
| ApaI | GGGCCC | 1 cut(s) | 212 |
| ApeKI | GCWGC | 3 cut(s) | 168, 271, 457 |
| AspS9I | GGNCC | 3 cut(s) | 208, 209, 434 |
| AsuC2I | CCSGG | 3 cut(s) | 96, 207, 432 |
| AsuHPI | GGTGA | 3 cut(s) | 91, 131, 355 |
| AvaI | CYCGRG | 3 cut(s) | 212, 461, 474 |
| AvaII | GGWCC | 1 cut(s) | 434 |
| BaeGI | GKGCMC | 1 cut(s) | 212 |
| BanII | GRGCYC | 3 cut(s) | 49, 212, 475 |
| BbvI | GCAGC | 3 cut(s) | 155, 258, 444 |
| BccI | CCATC | 3 cut(s) | 74, 116, 280 |
| BceAI | ACGGC | 2 cut(s) | 144, 375 |
| BcnI | CCSGG | 3 cut(s) | 96, 207, 432 |
| BcoDI | GTCTC | 2 cut(s) | 207, 398 |
| BfaI | CTAG | 1 cut(s) | 6 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 10, 165 |
| BisI | GCNGC | 4 cut(s) | 14, 169, 272, 458 |
| BlsI | GCNGC | 4 cut(s) | 15, 170, 273, 459 |
| Bme1390I | CCNGG | 3 cut(s) | 96, 207, 432 |
| Bme18I | GGWCC | 1 cut(s) | 434 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 440 |
| BmeT110I | CYCGRG | 3 cut(s) | 212, 461, 474 |
| BmgT120I | GGNCC | 3 cut(s) | 208, 209, 434 |
| BmiI | GGNNCC | 5 cut(s) | 48, 59, 210, 211, 435 |
| BmrFI | CCNGG | 3 cut(s) | 96, 207, 432 |
| BmsI | GCATC | 2 cut(s) | 49, 377 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 212, 244 |
| BpuMI | CCSGG | 3 cut(s) | 96, 207, 432 |
| BsaBI | GATNNNNATC | 1 cut(s) | 243 |
| BsaHI | GRCGYC | 1 cut(s) | 252 |
| BsaI | GGTCTC | 1 cut(s) | 207 |
| BsaJI | CCNNGG | 1 cut(s) | 135 |
| BsaWI | WCCGGW | 2 cut(s) | 28, 396 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 68, 98 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 72, 319, 339, 461 |
| Bse118I | RCCGGY | 1 cut(s) | 54 |
| Bse8I | GATNNNNATC | 1 cut(s) | 243 |
| BseAI | TCCGGA | 2 cut(s) | 28, 396 |
| BseDI | CCNNGG | 1 cut(s) | 135 |
| BseGI | GGATG | 1 cut(s) | 291 |
| BseJI | GATNNNNATC | 1 cut(s) | 243 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 72, 319, 339, 461 |
| BseSI | GKGCMC | 1 cut(s) | 212 |
| BseXI | GCAGC | 3 cut(s) | 155, 258, 444 |
| Bsh1236I | CGCG | 1 cut(s) | 73 |
| BshFI | GGCC | 2 cut(s) | 210, 430 |
| BsiHKCI | CYCGRG | 3 cut(s) | 212, 461, 474 |
| BsiSI | CCGG | 6 cut(s) | 29, 55, 95, 206, 397, 431 |
| BslFI | GGGAC | 1 cut(s) | 447 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 72, 319, 339, 461 |
| BsmAI | GTCTC | 2 cut(s) | 207, 398 |
| BsmBI | CGTCTC | 1 cut(s) | 398 |
| BsmFI | GGGAC | 1 cut(s) | 447 |
| BsnI | GGCC | 2 cut(s) | 210, 430 |
| Bso31I | GGTCTC | 1 cut(s) | 207 |
| BsoBI | CYCGRG | 3 cut(s) | 212, 461, 474 |
| Bsp120I | GGGCCC | 1 cut(s) | 208 |
| Bsp1286I | GDGCHC | 3 cut(s) | 49, 212, 475 |
| Bsp13I | TCCGGA | 2 cut(s) | 28, 396 |
| Bsp143I | GATC | 2 cut(s) | 174, 244 |
| BspACI | CCGC | 5 cut(s) | 13, 16, 73, 101, 455 |
| BspANI | GGCC | 2 cut(s) | 210, 430 |
| BspEI | TCCGGA | 2 cut(s) | 28, 396 |
| BspFNI | CGCG | 1 cut(s) | 73 |
| BspHI | TCATGA | 1 cut(s) | 444 |
| BspLI | GGNNCC | 5 cut(s) | 48, 59, 210, 211, 435 |
| BspPI | GGATC | 1 cut(s) | 239 |
| BspTNI | GGTCTC | 1 cut(s) | 207 |
| BsrBI | CCGCTC | 1 cut(s) | 101 |
| BsrFI | RCCGGY | 1 cut(s) | 54 |
| BssAI | RCCGGY | 1 cut(s) | 54 |
| BssECI | CCNNGG | 1 cut(s) | 135 |
| BssMI | GATC | 2 cut(s) | 174, 244 |
| BssNI | GRCGYC | 1 cut(s) | 252 |
| BssT1I | CCWWGG | 1 cut(s) | 135 |
| Bst4CI | ACNGT | 1 cut(s) | 322 |
| BstACI | GRCGYC | 1 cut(s) | 252 |
| BstF5I | GGATG | 1 cut(s) | 291 |
| BstFNI | CGCG | 1 cut(s) | 73 |
| BstKTI | GATC | 2 cut(s) | 177, 247 |
| BstMAI | GTCTC | 2 cut(s) | 207, 398 |
| BstMBI | GATC | 2 cut(s) | 174, 244 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 10, 19, 165, 234 |
| BstSCI | CCNGG | 3 cut(s) | 94, 205, 430 |
| BstSLI | GKGCMC | 1 cut(s) | 212 |
| BstUI | CGCG | 1 cut(s) | 73 |
| BstV1I | GCAGC | 3 cut(s) | 155, 258, 444 |
| BsuRI | GGCC | 2 cut(s) | 210, 430 |
| BtsCI | GGATG | 1 cut(s) | 291 |
| CciI | TCATGA | 1 cut(s) | 444 |
| Cfr10I | RCCGGY | 1 cut(s) | 54 |
| Cfr13I | GGNCC | 3 cut(s) | 208, 209, 434 |
| CseI | GACGC | 1 cut(s) | 234 |
| CviAII | CATG | 1 cut(s) | 445 |
| CviJI | RGCY | 7 cut(s) | 47, 54, 107, 168, 210, 430, 473 |
| CviKI_1 | RGCY | 7 cut(s) | 47, 54, 107, 168, 210, 430, 473 |
| DpnI | GATC | 2 cut(s) | 176, 246 |
| DpnII | GATC | 2 cut(s) | 174, 244 |
| DriI | GACNNNNNGTC | 1 cut(s) | 440 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 440 |
| Eco130I | CCWWGG | 1 cut(s) | 135 |
| Eco24I | GRGCYC | 3 cut(s) | 49, 212, 475 |
| Eco31I | GGTCTC | 1 cut(s) | 207 |
| Eco47I | GGWCC | 1 cut(s) | 434 |
| Eco88I | CYCGRG | 3 cut(s) | 212, 461, 474 |
| EcoO109I | RGGNCCY | 1 cut(s) | 209 |
| EcoT14I | CCWWGG | 1 cut(s) | 135 |
| EcoT38I | GRGCYC | 3 cut(s) | 49, 212, 475 |
| ErhI | CCWWGG | 1 cut(s) | 135 |
| Esp3I | CGTCTC | 1 cut(s) | 398 |
| FaeI | CATG | 1 cut(s) | 448 |
| FaiI | YATR | 6 cut(s) | 35, 180, 233, 235, 350, 446 |
| FaqI | GGGAC | 1 cut(s) | 447 |
| FatI | CATG | 1 cut(s) | 444 |
| FauI | CCCGC | 2 cut(s) | 66, 462 |
| FauNDI | CATATG | 1 cut(s) | 233 |
| FblI | GTMKAC | 1 cut(s) | 306 |
| Fnu4HI | GCNGC | 4 cut(s) | 14, 169, 272, 458 |
| FokI | GGATG | 1 cut(s) | 298 |
| FriOI | GRGCYC | 3 cut(s) | 49, 212, 475 |
| Fsp4HI | GCNGC | 4 cut(s) | 14, 169, 272, 458 |
| FspBI | CTAG | 1 cut(s) | 6 |
| GluI | GCNGC | 4 cut(s) | 14, 169, 272, 458 |
| HaeIII | GGCC | 2 cut(s) | 210, 430 |
| HapII | CCGG | 6 cut(s) | 29, 55, 95, 206, 397, 431 |
| HgaI | GACGC | 1 cut(s) | 234 |
| Hin1I | GRCGYC | 1 cut(s) | 252 |
| Hin1II | CATG | 1 cut(s) | 448 |
| HincII | GTYRAC | 1 cut(s) | 87 |
| HindII | GTYRAC | 1 cut(s) | 87 |
| HindIII | AAGCTT | 1 cut(s) | 105 |
| HinfI | GANTC | 3 cut(s) | 278, 379, 416 |
| HpaII | CCGG | 6 cut(s) | 29, 55, 95, 206, 397, 431 |
| HphI | GGTGA | 3 cut(s) | 91, 131, 355 |
| Hpy166II | GTNNAC | 2 cut(s) | 87, 307 |
| Hpy188I | TCNGA | 1 cut(s) | 51 |
| Hpy188III | TCNNGA | 7 cut(s) | 29, 248, 374, 397, 420, 445, 485 |
| Hpy8I | GTNNAC | 2 cut(s) | 87, 307 |
| Hpy99I | CGWCG | 2 cut(s) | 228, 254 |
| HpyAV | CCTTC | 2 cut(s) | 417, 433 |
| HpyCH4III | ACNGT | 1 cut(s) | 322 |
| HpyCH4IV | ACGT | 1 cut(s) | 252 |
| HpyCH4V | TGCA | 1 cut(s) | 40 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 10, 19, 165, 234 |
| HpySE526I | ACGT | 1 cut(s) | 252 |
| Hsp92I | GRCGYC | 1 cut(s) | 252 |
| Hsp92II | CATG | 1 cut(s) | 448 |
| Kpn2I | TCCGGA | 2 cut(s) | 28, 396 |
| Kzo9I | GATC | 2 cut(s) | 174, 244 |
| LmnI | GCTCC | 3 cut(s) | 52, 98, 233 |
| LpnPI | CCDG | 6 cut(s) | 42, 68, 108, 219, 410, 444 |
| Lsp1109I | GCAGC | 3 cut(s) | 155, 258, 444 |
| LweI | GCATC | 2 cut(s) | 49, 377 |
| MaeI | CTAG | 1 cut(s) | 6 |
| MaeII | ACGT | 1 cut(s) | 252 |
| MaeIII | GTNAC | 1 cut(s) | 328 |
| MalI | GATC | 2 cut(s) | 176, 246 |
| MbiI | CCGCTC | 1 cut(s) | 101 |
| MboI | GATC | 2 cut(s) | 174, 244 |
| MhlI | GDGCHC | 3 cut(s) | 49, 212, 475 |
| MluCI | AATT | 1 cut(s) | 297 |
| MlyI | GAGTC | 1 cut(s) | 425 |
| MmeI | TCCRAC | 1 cut(s) | 85 |
| MnlI | CCTC | 7 cut(s) | 70, 109, 138, 192, 285, 320, 420 |
| MroI | TCCGGA | 2 cut(s) | 28, 396 |
| MseI | TTAA | 1 cut(s) | 194 |
| MspA1I | CMGCKG | 1 cut(s) | 457 |
| MspI | CCGG | 6 cut(s) | 29, 55, 95, 206, 397, 431 |
| MspR9I | CCNGG | 3 cut(s) | 96, 207, 432 |
| MvnI | CGCG | 1 cut(s) | 73 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 10, 19, 165, 234 |
| NciI | CCSGG | 3 cut(s) | 96, 207, 432 |
| NdeI | CATATG | 1 cut(s) | 233 |
| NdeII | GATC | 2 cut(s) | 174, 244 |
| NlaIII | CATG | 1 cut(s) | 448 |
| NlaIV | GGNNCC | 5 cut(s) | 48, 59, 210, 211, 435 |
| PagI | TCATGA | 1 cut(s) | 444 |
| PfeI | GAWTC | 2 cut(s) | 278, 379 |
| PkrI | GCNGC | 4 cut(s) | 15, 170, 273, 459 |
| PleI | GAGTC | 1 cut(s) | 424 |
| PpsI | GAGTC | 1 cut(s) | 424 |
| PspN4I | GGNNCC | 5 cut(s) | 48, 59, 210, 211, 435 |
| PspOMI | GGGCCC | 1 cut(s) | 208 |
| PspPI | GGNCC | 3 cut(s) | 208, 209, 434 |
| SaqAI | TTAA | 1 cut(s) | 194 |
| SatI | GCNGC | 4 cut(s) | 14, 169, 272, 458 |
| Sau3AI | GATC | 2 cut(s) | 174, 244 |
| Sau96I | GGNCC | 3 cut(s) | 208, 209, 434 |
| SchI | GAGTC | 1 cut(s) | 425 |
| ScrFI | CCNGG | 3 cut(s) | 96, 207, 432 |
| SduI | GDGCHC | 3 cut(s) | 49, 212, 475 |
| SetI | ASST | 6 cut(s) | 81, 109, 203, 255, 312, 444 |
| SfaNI | GCATC | 2 cut(s) | 49, 377 |
| SinI | GGWCC | 1 cut(s) | 434 |
| Sse9I | AATT | 1 cut(s) | 297 |
| SsiI | CCGC | 5 cut(s) | 13, 16, 73, 101, 455 |
| SspMI | CTAG | 1 cut(s) | 6 |
| StyD4I | CCNGG | 3 cut(s) | 94, 205, 430 |
| StyI | CCWWGG | 1 cut(s) | 135 |
| TaaI | ACNGT | 1 cut(s) | 322 |
| TaiI | ACGT | 1 cut(s) | 255 |
| TaqI | TCGA | 1 cut(s) | 373 |
| TaqII | GACCGA | 1 cut(s) | 451 |
| TasI | AATT | 1 cut(s) | 297 |
| TauI | GCSGC | 1 cut(s) | 16 |
| TfiI | GAWTC | 2 cut(s) | 278, 379 |
| Tru1I | TTAA | 1 cut(s) | 194 |
| Tru9I | TTAA | 1 cut(s) | 194 |
| TseI | GCWGC | 3 cut(s) | 168, 271, 457 |
| TspDTI | ATGAA | 1 cut(s) | 461 |
| TspGWI | ACGGA | 1 cut(s) | 298 |
| VpaK11BI | GGWCC | 1 cut(s) | 434 |
| XmiI | GTMKAC | 1 cut(s) | 306 |
| XspI | CTAG | 1 cut(s) | 6 |
| ZraI | GACGTC | 1 cut(s) | 253 |