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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 750 |
| AccII | CGCG | 1 cut(s) | 588 |
| AciI | CCGC | 3 cut(s) | 388, 586, 633 |
| AclWI | GGATC | 5 cut(s) | 197, 210, 547, 912, 988 |
| AcoI | YGGCCR | 2 cut(s) | 476, 583 |
| AcuI | CTGAAG | 1 cut(s) | 716 |
| AfaI | GTAC | 3 cut(s) | 375, 676, 842 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 108, 265, 592, 773, 1003 |
| AflII | CTTAAG | 1 cut(s) | 932 |
| AgsI | TTSAA | 6 cut(s) | 47, 302, 511, 526, 794, 971 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 166 |
| AjnI | CCWGG | 1 cut(s) | 478 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 78, 110 |
| AleI | CACNNNNGTG | 1 cut(s) | 686 |
| Alw21I | GWGCWC | 3 cut(s) | 155, 1011, 1019 |
| Alw26I | GTCTC | 1 cut(s) | 197 |
| AlwI | GGATC | 5 cut(s) | 197, 210, 547, 912, 988 |
| AoxI | GGCC | 5 cut(s) | 123, 178, 213, 476, 583 |
| ApeKI | GCWGC | 2 cut(s) | 916, 985 |
| AspS9I | GGNCC | 3 cut(s) | 5, 214, 390 |
| AsuC2I | CCSGG | 1 cut(s) | 715 |
| AsuHPI | GGTGA | 2 cut(s) | 562, 607 |
| AvaII | GGWCC | 2 cut(s) | 5, 390 |
| AxyI | CCTNAGG | 1 cut(s) | 926 |
| BalI | TGGCCA | 1 cut(s) | 478 |
| BamHI | GGATCC | 1 cut(s) | 202 |
| BanII | GRGCYC | 3 cut(s) | 103, 1011, 1019 |
| Bbv12I | GWGCWC | 3 cut(s) | 155, 1011, 1019 |
| BbvI | GCAGC | 2 cut(s) | 903, 997 |
| BceAI | ACGGC | 2 cut(s) | 243, 383 |
| BciT130I | CCWGG | 1 cut(s) | 480 |
| BcnI | CCSGG | 1 cut(s) | 715 |
| BcoDI | GTCTC | 1 cut(s) | 197 |
| BfaI | CTAG | 2 cut(s) | 182, 920 |
| BfrI | CTTAAG | 1 cut(s) | 932 |
| BisI | GCNGC | 4 cut(s) | 586, 633, 917, 986 |
| BlpI | GCTNAGC | 2 cut(s) | 615, 912 |
| BlsI | GCNGC | 4 cut(s) | 587, 634, 918, 987 |
| BmcAI | AGTACT | 1 cut(s) | 676 |
| Bme1390I | CCNGG | 2 cut(s) | 480, 715 |
| Bme18I | GGWCC | 2 cut(s) | 5, 390 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 166 |
| BmgT120I | GGNCC | 3 cut(s) | 5, 214, 390 |
| BmiI | GGNNCC | 4 cut(s) | 6, 100, 204, 670 |
| BmrFI | CCNGG | 2 cut(s) | 480, 715 |
| BmsI | GCATC | 4 cut(s) | 340, 694, 700, 787 |
| Bpu10I | CCTNAGC | 1 cut(s) | 9 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 615, 912 |
| BpuEI | CTTGAG | 1 cut(s) | 995 |
| BpuMI | CCSGG | 1 cut(s) | 715 |
| BsaBI | GATNNNNATC | 1 cut(s) | 903 |
| BsaI | GGTCTC | 1 cut(s) | 197 |
| BsaJI | CCNNGG | 5 cut(s) | 126, 198, 206, 361, 479 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 108, 265, 592, 773, 1003 |
| Bse1I | ACTGG | 2 cut(s) | 656, 672 |
| Bse21I | CCTNAGG | 1 cut(s) | 926 |
| Bse8I | GATNNNNATC | 1 cut(s) | 903 |
| BseBI | CCWGG | 1 cut(s) | 480 |
| BseDI | CCNNGG | 5 cut(s) | 126, 198, 206, 361, 479 |
| BseGI | GGATG | 3 cut(s) | 88, 867, 904 |
| BseJI | GATNNNNATC | 1 cut(s) | 903 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 108, 265, 592, 773, 1003 |
| BseMII | CTCAG | 4 cut(s) | 51, 606, 878, 903 |
| BseNI | ACTGG | 2 cut(s) | 656, 672 |
| BseRI | GAGGAG | 4 cut(s) | 308, 590, 759, 995 |
| BseXI | GCAGC | 2 cut(s) | 903, 997 |
| BseYI | CCCAGC | 1 cut(s) | 314 |
| Bsh1236I | CGCG | 1 cut(s) | 588 |
| BshFI | GGCC | 5 cut(s) | 125, 180, 215, 478, 585 |
| BsiHKAI | GWGCWC | 3 cut(s) | 155, 1011, 1019 |
| BsiSI | CCGG | 2 cut(s) | 109, 715 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 108, 265, 592, 773, 1003 |
| BsmAI | GTCTC | 1 cut(s) | 197 |
| BsmI | GAATGC | 1 cut(s) | 762 |
| BsnI | GGCC | 5 cut(s) | 125, 180, 215, 478, 585 |
| Bso31I | GGTCTC | 1 cut(s) | 197 |
| Bsp1286I | GDGCHC | 4 cut(s) | 103, 155, 1011, 1019 |
| Bsp143I | GATC | 6 cut(s) | 172, 202, 274, 552, 904, 993 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 615, 912 |
| BspACI | CCGC | 3 cut(s) | 388, 586, 633 |
| BspANI | GGCC | 5 cut(s) | 125, 180, 215, 478, 585 |
| BspCNI | CTCAG | 4 cut(s) | 52, 607, 879, 904 |
| BspFNI | CGCG | 1 cut(s) | 588 |
| BspLI | GGNNCC | 4 cut(s) | 6, 100, 204, 670 |
| BspPI | GGATC | 5 cut(s) | 197, 210, 547, 912, 988 |
| BspQI | GCTCTTC | 2 cut(s) | 143, 843 |
| BspTI | CTTAAG | 1 cut(s) | 932 |
| BspTNI | GGTCTC | 1 cut(s) | 197 |
| BsrI | ACTGG | 2 cut(s) | 656, 672 |
| BssECI | CCNNGG | 5 cut(s) | 126, 198, 206, 361, 479 |
| BssMI | GATC | 6 cut(s) | 172, 202, 274, 552, 904, 993 |
| BssT1I | CCWWGG | 3 cut(s) | 126, 198, 361 |
| Bst2UI | CCWGG | 1 cut(s) | 480 |
| Bst4CI | ACNGT | 3 cut(s) | 55, 564, 668 |
| Bst6I | CTCTTC | 6 cut(s) | 143, 297, 430, 638, 734, 843 |
| BstAFI | CTTAAG | 1 cut(s) | 932 |
| BstC8I | GCNNGC | 2 cut(s) | 15, 723 |
| BstDEI | CTNAG | 7 cut(s) | 9, 60, 615, 887, 912, 926, 1020 |
| BstDSI | CCRYGG | 1 cut(s) | 206 |
| BstF5I | GGATG | 3 cut(s) | 88, 867, 904 |
| BstFNI | CGCG | 1 cut(s) | 588 |
| BstKTI | GATC | 6 cut(s) | 175, 205, 277, 555, 907, 996 |
| BstMAI | GTCTC | 1 cut(s) | 197 |
| BstMBI | GATC | 6 cut(s) | 172, 202, 274, 552, 904, 993 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 23, 611, 632, 697 |
| BstNI | CCWGG | 1 cut(s) | 480 |
| BstSCI | CCNGG | 2 cut(s) | 478, 713 |
| BstUI | CGCG | 1 cut(s) | 588 |
| BstV1I | GCAGC | 2 cut(s) | 903, 997 |
| BstX2I | RGATCY | 3 cut(s) | 202, 904, 993 |
| BstYI | RGATCY | 3 cut(s) | 202, 904, 993 |
| Bsu36I | CCTNAGG | 1 cut(s) | 926 |
| BsuRI | GGCC | 5 cut(s) | 125, 180, 215, 478, 585 |
| BtgI | CCRYGG | 1 cut(s) | 206 |
| BtsCI | GGATG | 3 cut(s) | 88, 867, 904 |
| BtsIMutI | CAGTG | 2 cut(s) | 51, 560 |
| Cac8I | GCNNGC | 2 cut(s) | 15, 723 |
| Cfr13I | GGNCC | 3 cut(s) | 5, 214, 390 |
| Csp6I | GTAC | 3 cut(s) | 374, 675, 841 |
| CviAII | CATG | 2 cut(s) | 176, 611 |
| CviQI | GTAC | 3 cut(s) | 374, 675, 841 |
| DdeI | CTNAG | 7 cut(s) | 9, 60, 615, 887, 912, 926, 1020 |
| DpnI | GATC | 6 cut(s) | 174, 204, 276, 554, 906, 995 |
| DpnII | GATC | 6 cut(s) | 172, 202, 274, 552, 904, 993 |
| DriI | GACNNNNNGTC | 1 cut(s) | 166 |
| EaeI | YGGCCR | 2 cut(s) | 476, 583 |
| Eam1104I | CTCTTC | 6 cut(s) | 143, 297, 430, 638, 734, 843 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 166 |
| EarI | CTCTTC | 6 cut(s) | 143, 297, 430, 638, 734, 843 |
| EciI | GGCGGA | 1 cut(s) | 403 |
| Ecl136II | GAGCTC | 2 cut(s) | 1009, 1017 |
| Eco130I | CCWWGG | 3 cut(s) | 126, 198, 361 |
| Eco24I | GRGCYC | 3 cut(s) | 103, 1011, 1019 |
| Eco31I | GGTCTC | 1 cut(s) | 197 |
| Eco47I | GGWCC | 2 cut(s) | 5, 390 |
| Eco53kI | GAGCTC | 2 cut(s) | 1009, 1017 |
| Eco57I | CTGAAG | 1 cut(s) | 716 |
| Eco81I | CCTNAGG | 1 cut(s) | 926 |
| EcoICRI | GAGCTC | 2 cut(s) | 1009, 1017 |
| EcoO109I | RGGNCCY | 1 cut(s) | 5 |
| EcoRII | CCWGG | 1 cut(s) | 478 |
| EcoT14I | CCWWGG | 3 cut(s) | 126, 198, 361 |
| EcoT38I | GRGCYC | 3 cut(s) | 103, 1011, 1019 |
| ErhI | CCWWGG | 3 cut(s) | 126, 198, 361 |
| FaeI | CATG | 2 cut(s) | 179, 614 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 503, 535, 786, 818 |
| FatI | CATG | 2 cut(s) | 175, 610 |
| FauNDI | CATATG | 2 cut(s) | 571, 771 |
| FblI | GTMKAC | 1 cut(s) | 750 |
| Fnu4HI | GCNGC | 4 cut(s) | 586, 633, 917, 986 |
| FokI | GGATG | 3 cut(s) | 95, 874, 911 |
| FriOI | GRGCYC | 3 cut(s) | 103, 1011, 1019 |
| Fsp4HI | GCNGC | 4 cut(s) | 586, 633, 917, 986 |
| FspBI | CTAG | 2 cut(s) | 182, 920 |
| GluI | GCNGC | 4 cut(s) | 586, 633, 917, 986 |
| GsaI | CCCAGC | 1 cut(s) | 318 |
| HaeIII | GGCC | 5 cut(s) | 125, 180, 215, 478, 585 |
| HapII | CCGG | 2 cut(s) | 109, 715 |
| Hin1II | CATG | 2 cut(s) | 179, 614 |
| HincII | GTYRAC | 1 cut(s) | 751 |
| HindII | GTYRAC | 1 cut(s) | 751 |
| HindIII | AAGCTT | 1 cut(s) | 719 |
| HinfI | GANTC | 2 cut(s) | 747, 1001 |
| HpaII | CCGG | 2 cut(s) | 109, 715 |
| HphI | GGTGA | 2 cut(s) | 562, 607 |
| Hpy166II | GTNNAC | 2 cut(s) | 567, 751 |
| Hpy188I | TCNGA | 5 cut(s) | 172, 696, 837, 998, 1006 |
| Hpy188III | TCNNGA | 5 cut(s) | 403, 511, 526, 556, 1012 |
| Hpy8I | GTNNAC | 2 cut(s) | 567, 751 |
| Hpy99I | CGWCG | 1 cut(s) | 755 |
| HpyAV | CCTTC | 5 cut(s) | 80, 249, 535, 592, 851 |
| HpyCH4III | ACNGT | 3 cut(s) | 55, 564, 668 |
| HpyCH4IV | ACGT | 1 cut(s) | 839 |
| HpyCH4V | TGCA | 3 cut(s) | 664, 691, 762 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 23, 611, 632, 697 |
| HpyF3I | CTNAG | 7 cut(s) | 9, 60, 615, 887, 912, 926, 1020 |
| HpySE526I | ACGT | 1 cut(s) | 839 |
| Hsp92II | CATG | 2 cut(s) | 179, 614 |
| Kzo9I | GATC | 6 cut(s) | 172, 202, 274, 552, 904, 993 |
| LguI | GCTCTTC | 2 cut(s) | 143, 843 |
| LmnI | GCTCC | 4 cut(s) | 98, 331, 982, 1006 |
| Lsp1109I | GCAGC | 2 cut(s) | 903, 997 |
| LweI | GCATC | 4 cut(s) | 340, 694, 700, 787 |
| MaeI | CTAG | 2 cut(s) | 182, 920 |
| MaeII | ACGT | 1 cut(s) | 839 |
| MaeIII | GTNAC | 1 cut(s) | 957 |
| MalI | GATC | 6 cut(s) | 174, 204, 276, 554, 906, 995 |
| MboI | GATC | 6 cut(s) | 172, 202, 274, 552, 904, 993 |
| MflI | RGATCY | 3 cut(s) | 202, 904, 993 |
| MhlI | GDGCHC | 4 cut(s) | 103, 155, 1011, 1019 |
| MlsI | TGGCCA | 1 cut(s) | 478 |
| MluCI | AATT | 1 cut(s) | 972 |
| MluNI | TGGCCA | 1 cut(s) | 478 |
| MlyI | GAGTC | 1 cut(s) | 756 |
| MmeI | TCCRAC | 1 cut(s) | 427 |
| Mox20I | TGGCCA | 1 cut(s) | 478 |
| MscI | TGGCCA | 1 cut(s) | 478 |
| MseI | TTAA | 3 cut(s) | 285, 933, 954 |
| MslI | CAYNNNNRTG | 1 cut(s) | 686 |
| Msp20I | TGGCCA | 1 cut(s) | 478 |
| MspCI | CTTAAG | 1 cut(s) | 932 |
| MspI | CCGG | 2 cut(s) | 109, 715 |
| MspR9I | CCNGG | 2 cut(s) | 480, 715 |
| Mva1269I | GAATGC | 1 cut(s) | 762 |
| MvaI | CCWGG | 1 cut(s) | 480 |
| MvnI | CGCG | 1 cut(s) | 588 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 23, 611, 632, 697 |
| NciI | CCSGG | 1 cut(s) | 715 |
| NdeI | CATATG | 2 cut(s) | 571, 771 |
| NdeII | GATC | 6 cut(s) | 172, 202, 274, 552, 904, 993 |
| NlaIII | CATG | 2 cut(s) | 179, 614 |
| NlaIV | GGNNCC | 4 cut(s) | 6, 100, 204, 670 |
| NmeAIII | GCCGAG | 1 cut(s) | 661 |
| OliI | CACNNNNGTG | 1 cut(s) | 686 |
| PciSI | GCTCTTC | 2 cut(s) | 143, 843 |
| PctI | GAATGC | 1 cut(s) | 762 |
| PfeI | GAWTC | 1 cut(s) | 1001 |
| PflFI | GACNNNGTC | 1 cut(s) | 562 |
| PfoI | TCCNGGA | 1 cut(s) | 713 |
| PkrI | GCNGC | 4 cut(s) | 587, 634, 918, 987 |
| PleI | GAGTC | 1 cut(s) | 755 |
| PpsI | GAGTC | 1 cut(s) | 755 |
| PpuMI | RGGWCCY | 1 cut(s) | 5 |
| Psp124BI | GAGCTC | 2 cut(s) | 1011, 1019 |
| Psp5II | RGGWCCY | 1 cut(s) | 5 |
| Psp6I | CCWGG | 1 cut(s) | 478 |
| PspFI | CCCAGC | 1 cut(s) | 314 |
| PspGI | CCWGG | 1 cut(s) | 478 |
| PspN4I | GGNNCC | 4 cut(s) | 6, 100, 204, 670 |
| PspPI | GGNCC | 3 cut(s) | 5, 214, 390 |
| PspPPI | RGGWCCY | 1 cut(s) | 5 |
| PsuI | RGATCY | 3 cut(s) | 202, 904, 993 |
| PsyI | GACNNNGTC | 1 cut(s) | 562 |
| RsaI | GTAC | 3 cut(s) | 375, 676, 842 |
| RsaNI | GTAC | 3 cut(s) | 374, 675, 841 |
| RseI | CAYNNNNRTG | 1 cut(s) | 686 |
| SacI | GAGCTC | 2 cut(s) | 1011, 1019 |
| SalI | GTCGAC | 1 cut(s) | 749 |
| SapI | GCTCTTC | 2 cut(s) | 143, 843 |
| SaqAI | TTAA | 3 cut(s) | 285, 933, 954 |
| SatI | GCNGC | 4 cut(s) | 586, 633, 917, 986 |
| Sau3AI | GATC | 6 cut(s) | 172, 202, 274, 552, 904, 993 |
| Sau96I | GGNCC | 3 cut(s) | 5, 214, 390 |
| ScaI | AGTACT | 1 cut(s) | 676 |
| SchI | GAGTC | 1 cut(s) | 756 |
| ScrFI | CCNGG | 2 cut(s) | 480, 715 |
| SduI | GDGCHC | 4 cut(s) | 103, 155, 1011, 1019 |
| SfaNI | GCATC | 4 cut(s) | 340, 694, 700, 787 |
| SinI | GGWCC | 2 cut(s) | 5, 390 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 686 |
| SmlI | CTYRAG | 2 cut(s) | 932, 1010 |
| SmoI | CTYRAG | 2 cut(s) | 932, 1010 |
| Sse9I | AATT | 1 cut(s) | 972 |
| SsiI | CCGC | 3 cut(s) | 388, 586, 633 |
| SspI | AATATT | 1 cut(s) | 758 |
| SspMI | CTAG | 2 cut(s) | 182, 920 |
| SstI | GAGCTC | 2 cut(s) | 1011, 1019 |
| StyD4I | CCNGG | 2 cut(s) | 478, 713 |
| StyI | CCWWGG | 3 cut(s) | 126, 198, 361 |
| TaaI | ACNGT | 3 cut(s) | 55, 564, 668 |
| TaiI | ACGT | 1 cut(s) | 842 |
| TaqI | TCGA | 2 cut(s) | 750, 964 |
| TasI | AATT | 1 cut(s) | 972 |
| TatI | WGTACW | 2 cut(s) | 373, 674 |
| TauI | GCSGC | 2 cut(s) | 588, 635 |
| TfiI | GAWTC | 1 cut(s) | 1001 |
| Tru1I | TTAA | 3 cut(s) | 285, 933, 954 |
| Tru9I | TTAA | 3 cut(s) | 285, 933, 954 |
| TscAI | CASTG | 2 cut(s) | 58, 567 |
| TseI | GCWGC | 2 cut(s) | 916, 985 |
| TspDTI | ATGAA | 3 cut(s) | 99, 878, 882 |
| TspRI | CASTG | 2 cut(s) | 58, 567 |
| Tth111I | GACNNNGTC | 1 cut(s) | 562 |
| Vha464I | CTTAAG | 1 cut(s) | 932 |
| VpaK11BI | GGWCC | 2 cut(s) | 5, 390 |
| XmiI | GTMKAC | 1 cut(s) | 750 |
| XspI | CTAG | 2 cut(s) | 182, 920 |
| ZrmI | AGTACT | 1 cut(s) | 676 |