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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 3 cut(s) | 513, 898, 1667 |
| AatII | GACGTC | 1 cut(s) | 147 |
| Acc16I | TGCGCA | 1 cut(s) | 976 |
| Acc36I | ACCTGC | 1 cut(s) | 1189 |
| AccB1I | GGYRCC | 3 cut(s) | 368, 554, 741 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 963 |
| AccBSI | CCGCTC | 1 cut(s) | 1219 |
| AccI | GTMKAC | 2 cut(s) | 84, 207 |
| AclWI | GGATC | 3 cut(s) | 977, 1009, 1618 |
| AcoI | YGGCCR | 1 cut(s) | 1084 |
| AcuI | CTGAAG | 3 cut(s) | 89, 409, 1233 |
| AcyI | GRCGYC | 2 cut(s) | 144, 767 |
| AfaI | GTAC | 3 cut(s) | 488, 851, 1456 |
| AgsI | TTSAA | 7 cut(s) | 166, 293, 659, 754, 1036, 1247, 1322 |
| AjiI | CACGTC | 1 cut(s) | 650 |
| AjnI | CCWGG | 4 cut(s) | 14, 737, 913, 1065 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 639, 671 |
| AluBI | AGCT | 3 cut(s) | 1063, 1270, 1298 |
| AluI | AGCT | 3 cut(s) | 1063, 1270, 1298 |
| Alw21I | GWGCWC | 2 cut(s) | 1057, 1065 |
| Alw26I | GTCTC | 5 cut(s) | 218, 484, 496, 1490, 1524 |
| AlwI | GGATC | 3 cut(s) | 977, 1009, 1618 |
| Ama87I | CYCGRG | 2 cut(s) | 364, 1446 |
| AoxI | GGCC | 4 cut(s) | 705, 1084, 1485, 1707 |
| ApeKI | GCWGC | 3 cut(s) | 545, 1222, 1280 |
| AsiSI | GCGATCGC | 1 cut(s) | 999 |
| AspLEI | GCGC | 5 cut(s) | 328, 697, 860, 977, 1383 |
| AspS9I | GGNCC | 6 cut(s) | 706, 831, 907, 1201, 1485, 1669 |
| AsuHPI | GGTGA | 5 cut(s) | 145, 193, 353, 391, 1403 |
| AvaI | CYCGRG | 2 cut(s) | 364, 1446 |
| AvaII | GGWCC | 4 cut(s) | 831, 907, 1201, 1669 |
| BaeGI | GKGCMC | 3 cut(s) | 371, 559, 746 |
| BanI | GGYRCC | 3 cut(s) | 368, 554, 741 |
| BanII | GRGCYC | 1 cut(s) | 1065 |
| BauI | CACGAG | 1 cut(s) | 490 |
| Bbv12I | GWGCWC | 2 cut(s) | 1057, 1065 |
| BbvCI | CCTCAGC | 1 cut(s) | 129 |
| BbvI | GCAGC | 3 cut(s) | 557, 1209, 1292 |
| BccI | CCATC | 4 cut(s) | 666, 937, 1135, 1660 |
| BceAI | ACGGC | 1 cut(s) | 780 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 345, 379, 606, 640, 1112, 1146 |
| BciT130I | CCWGG | 4 cut(s) | 16, 739, 915, 1067 |
| BclI | TGATCA | 2 cut(s) | 1093, 1654 |
| BcoDI | GTCTC | 5 cut(s) | 218, 484, 496, 1490, 1524 |
| BfaI | CTAG | 5 cut(s) | 639, 710, 782, 1452, 1732 |
| BfoI | RGCGCY | 3 cut(s) | 329, 698, 1384 |
| BfuAI | ACCTGC | 1 cut(s) | 1189 |
| BisI | GCNGC | 8 cut(s) | 538, 546, 677, 1084, 1220, 1223, 1281, 1488 |
| BlsI | GCNGC | 8 cut(s) | 539, 547, 678, 1085, 1221, 1224, 1282, 1489 |
| BmcAI | AGTACT | 1 cut(s) | 488 |
| Bme1390I | CCNGG | 4 cut(s) | 16, 739, 915, 1067 |
| Bme18I | GGWCC | 4 cut(s) | 831, 907, 1201, 1669 |
| BmeT110I | CYCGRG | 2 cut(s) | 364, 1446 |
| BmgBI | CACGTC | 1 cut(s) | 650 |
| BmgT120I | GGNCC | 6 cut(s) | 706, 831, 907, 1201, 1485, 1669 |
| BmiI | GGNNCC | 8 cut(s) | 275, 370, 433, 556, 743, 833, 909, 1612 |
| BmrFI | CCNGG | 4 cut(s) | 16, 739, 915, 1067 |
| BmrI | ACTGGG | 1 cut(s) | 478 |
| BmsI | GCATC | 5 cut(s) | 106, 524, 743, 1246, 1390 |
| BmuI | ACTGGG | 1 cut(s) | 478 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 493, 525, 1282, 1314 |
| BpmI | CTGGAG | 1 cut(s) | 1049 |
| Bpu10I | CCTNAGC | 1 cut(s) | 129 |
| BpuEI | CTTGAG | 2 cut(s) | 76, 418 |
| BsaHI | GRCGYC | 2 cut(s) | 144, 767 |
| BsaJI | CCNNGG | 4 cut(s) | 559, 746, 1066, 1488 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 1047, 1077, 1094, 1124, 1136, 1166 |
| Bse1I | ACTGG | 4 cut(s) | 372, 484, 622, 1393 |
| Bse3DI | GCAATG | 1 cut(s) | 199 |
| BseBI | CCWGG | 4 cut(s) | 16, 739, 915, 1067 |
| BseDI | CCNNGG | 4 cut(s) | 559, 746, 1066, 1488 |
| BseGI | GGATG | 4 cut(s) | 677, 734, 1017, 1146 |
| BseMI | GCAATG | 1 cut(s) | 199 |
| BseMII | CTCAG | 6 cut(s) | 120, 219, 455, 521, 524, 712 |
| BseNI | ACTGG | 4 cut(s) | 372, 484, 622, 1393 |
| BseRI | GAGGAG | 5 cut(s) | 47, 170, 1115, 1199, 1451 |
| BseSI | GKGCMC | 3 cut(s) | 371, 559, 746 |
| BseX3I | CGGCCG | 1 cut(s) | 1084 |
| BseXI | GCAGC | 3 cut(s) | 557, 1209, 1292 |
| BseYI | CCCAGC | 1 cut(s) | 92 |
| BsgI | GTGCAG | 1 cut(s) | 1217 |
| Bsh1285I | CGRYCG | 4 cut(s) | 683, 953, 999, 1087 |
| BshFI | GGCC | 4 cut(s) | 707, 1086, 1487, 1709 |
| BshNI | GGYRCC | 3 cut(s) | 368, 554, 741 |
| BsiEI | CGRYCG | 4 cut(s) | 683, 953, 999, 1087 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1057, 1065 |
| BsiHKCI | CYCGRG | 2 cut(s) | 364, 1446 |
| BslFI | GGGAC | 6 cut(s) | 377, 400, 774, 817, 893, 904 |
| BsmAI | GTCTC | 5 cut(s) | 218, 484, 496, 1490, 1524 |
| BsmBI | CGTCTC | 2 cut(s) | 496, 1490 |
| BsmFI | GGGAC | 6 cut(s) | 377, 400, 774, 817, 893, 904 |
| BsmI | GAATGC | 2 cut(s) | 1042, 1473 |
| BsnI | GGCC | 4 cut(s) | 707, 1086, 1487, 1709 |
| BsoBI | CYCGRG | 2 cut(s) | 364, 1446 |
| Bsp1286I | GDGCHC | 5 cut(s) | 371, 559, 746, 1057, 1065 |
| Bsp143I | GATC | 7 cut(s) | 950, 969, 996, 1014, 1093, 1623, 1654 |
| Bsp68I | TCGCGA | 1 cut(s) | 1718 |
| BspANI | GGCC | 4 cut(s) | 707, 1086, 1487, 1709 |
| BspCNI | CTCAG | 6 cut(s) | 121, 220, 456, 522, 523, 711 |
| BspHI | TCATGA | 1 cut(s) | 1096 |
| BspLI | GGNNCC | 8 cut(s) | 275, 370, 433, 556, 743, 833, 909, 1612 |
| BspMI | ACCTGC | 1 cut(s) | 1189 |
| BspPI | GGATC | 3 cut(s) | 977, 1009, 1618 |
| BspQI | GCTCTTC | 1 cut(s) | 1209 |
| BspT107I | GGYRCC | 3 cut(s) | 368, 554, 741 |
| BsrBI | CCGCTC | 1 cut(s) | 1219 |
| BsrDI | GCAATG | 1 cut(s) | 199 |
| BsrI | ACTGG | 4 cut(s) | 372, 484, 622, 1393 |
| BssECI | CCNNGG | 4 cut(s) | 559, 746, 1066, 1488 |
| BssMI | GATC | 7 cut(s) | 950, 969, 996, 1014, 1093, 1623, 1654 |
| BssNI | GRCGYC | 2 cut(s) | 144, 767 |
| BssSI | CACGAG | 1 cut(s) | 490 |
| BssT1I | CCWWGG | 2 cut(s) | 559, 746 |
| Bst2BI | CACGAG | 1 cut(s) | 490 |
| Bst2UI | CCWGG | 4 cut(s) | 16, 739, 915, 1067 |
| Bst4CI | ACNGT | 4 cut(s) | 391, 632, 1253, 1535 |
| Bst6I | CTCTTC | 2 cut(s) | 70, 1209 |
| BstACI | GRCGYC | 2 cut(s) | 144, 767 |
| BstDEI | CTNAG | 8 cut(s) | 108, 129, 228, 405, 464, 510, 530, 698 |
| BstDSI | CCRYGG | 1 cut(s) | 1488 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 642 |
| BstF5I | GGATG | 4 cut(s) | 677, 734, 1017, 1146 |
| BstH2I | RGCGCY | 3 cut(s) | 329, 698, 1384 |
| BstHHI | GCGC | 5 cut(s) | 328, 697, 860, 977, 1383 |
| BstKTI | GATC | 7 cut(s) | 953, 972, 999, 1017, 1096, 1626, 1657 |
| BstMAI | GTCTC | 5 cut(s) | 218, 484, 496, 1490, 1524 |
| BstMBI | GATC | 7 cut(s) | 950, 969, 996, 1014, 1093, 1623, 1654 |
| BstMCI | CGRYCG | 4 cut(s) | 683, 953, 999, 1087 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 125, 429, 554, 932, 946, 1715 |
| BstNI | CCWGG | 4 cut(s) | 16, 739, 915, 1067 |
| BstNSI | RCATGY | 1 cut(s) | 91 |
| BstSCI | CCNGG | 4 cut(s) | 14, 737, 913, 1065 |
| BstSLI | GKGCMC | 3 cut(s) | 371, 559, 746 |
| BstV1I | GCAGC | 3 cut(s) | 557, 1209, 1292 |
| BstX2I | RGATCY | 1 cut(s) | 969 |
| BstYI | RGATCY | 1 cut(s) | 969 |
| BstZI | CGGCCG | 1 cut(s) | 1084 |
| BsuRI | GGCC | 4 cut(s) | 707, 1086, 1487, 1709 |
| BtgI | CCRYGG | 1 cut(s) | 1488 |
| BtgZI | GCGATG | 1 cut(s) | 1064 |
| BtrI | CACGTC | 1 cut(s) | 650 |
| BtsCI | GGATG | 4 cut(s) | 677, 734, 1017, 1146 |
| BtsI | GCAGTG | 3 cut(s) | 363, 1170, 1206 |
| BtsIMutI | CAGTG | 7 cut(s) | 363, 518, 629, 706, 1170, 1206, 1540 |
| BtuMI | TCGCGA | 1 cut(s) | 1718 |
| BveI | ACCTGC | 1 cut(s) | 1189 |
| CciI | TCATGA | 1 cut(s) | 1096 |
| CfoI | GCGC | 5 cut(s) | 328, 697, 860, 977, 1383 |
| Cfr13I | GGNCC | 6 cut(s) | 706, 831, 907, 1201, 1485, 1669 |
| Cfr42I | CCGCGG | 1 cut(s) | 1491 |
| CseI | GACGC | 4 cut(s) | 594, 756, 1507, 1702 |
| Csp6I | GTAC | 3 cut(s) | 487, 850, 1455 |
| CviAII | CATG | 3 cut(s) | 88, 1058, 1097 |
| CviQI | GTAC | 3 cut(s) | 487, 850, 1455 |
| DdeI | CTNAG | 8 cut(s) | 108, 129, 228, 405, 464, 510, 530, 698 |
| DpnI | GATC | 7 cut(s) | 952, 971, 998, 1016, 1095, 1625, 1656 |
| DpnII | GATC | 7 cut(s) | 950, 969, 996, 1014, 1093, 1623, 1654 |
| DraI | TTTAAA | 1 cut(s) | 1173 |
| DrdI | GACNNNNNNGTC | 3 cut(s) | 513, 898, 1667 |
| DseDI | GACNNNNNNGTC | 3 cut(s) | 513, 898, 1667 |
| EaeI | YGGCCR | 1 cut(s) | 1084 |
| EagI | CGGCCG | 1 cut(s) | 1084 |
| Eam1104I | CTCTTC | 2 cut(s) | 70, 1209 |
| EarI | CTCTTC | 2 cut(s) | 70, 1209 |
| EciI | GGCGGA | 1 cut(s) | 982 |
| Ecl136II | GAGCTC | 1 cut(s) | 1063 |
| EclXI | CGGCCG | 1 cut(s) | 1084 |
| Eco130I | CCWWGG | 2 cut(s) | 559, 746 |
| Eco24I | GRGCYC | 1 cut(s) | 1065 |
| Eco47I | GGWCC | 4 cut(s) | 831, 907, 1201, 1669 |
| Eco52I | CGGCCG | 1 cut(s) | 1084 |
| Eco53kI | GAGCTC | 1 cut(s) | 1063 |
| Eco57I | CTGAAG | 3 cut(s) | 89, 409, 1233 |
| Eco88I | CYCGRG | 2 cut(s) | 364, 1446 |
| EcoICRI | GAGCTC | 1 cut(s) | 1063 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 642 |
| EcoO109I | RGGNCCY | 3 cut(s) | 706, 831, 907 |
| EcoRII | CCWGG | 4 cut(s) | 14, 737, 913, 1065 |
| EcoT14I | CCWWGG | 2 cut(s) | 559, 746 |
| EcoT38I | GRGCYC | 1 cut(s) | 1065 |
| ErhI | CCWWGG | 2 cut(s) | 559, 746 |
| Esp3I | CGTCTC | 2 cut(s) | 496, 1490 |
| FaeI | CATG | 3 cut(s) | 91, 1061, 1100 |
| FaqI | GGGAC | 6 cut(s) | 377, 400, 774, 817, 893, 904 |
| FatI | CATG | 3 cut(s) | 87, 1057, 1096 |
| FauI | CCCGC | 3 cut(s) | 541, 1268, 1476 |
| FauNDI | CATATG | 1 cut(s) | 959 |
| FbaI | TGATCA | 2 cut(s) | 1093, 1654 |
| FblI | GTMKAC | 2 cut(s) | 84, 207 |
| Fnu4HI | GCNGC | 8 cut(s) | 538, 546, 677, 1084, 1220, 1223, 1281, 1488 |
| FokI | GGATG | 4 cut(s) | 684, 721, 1024, 1153 |
| FriOI | GRGCYC | 1 cut(s) | 1065 |
| Fsp4HI | GCNGC | 8 cut(s) | 538, 546, 677, 1084, 1220, 1223, 1281, 1488 |
| FspBI | CTAG | 5 cut(s) | 639, 710, 782, 1452, 1732 |
| FspI | TGCGCA | 1 cut(s) | 976 |
| GlaI | GCGC | 5 cut(s) | 327, 696, 859, 976, 1382 |
| GluI | GCNGC | 8 cut(s) | 538, 546, 677, 1084, 1220, 1223, 1281, 1488 |
| GsaI | CCCAGC | 1 cut(s) | 96 |
| GsuI | CTGGAG | 1 cut(s) | 1049 |
| HaeII | RGCGCY | 3 cut(s) | 329, 698, 1384 |
| HaeIII | GGCC | 4 cut(s) | 707, 1086, 1487, 1709 |
| HgaI | GACGC | 4 cut(s) | 594, 756, 1507, 1702 |
| HhaI | GCGC | 5 cut(s) | 328, 697, 860, 977, 1383 |
| Hin1I | GRCGYC | 2 cut(s) | 144, 767 |
| Hin1II | CATG | 3 cut(s) | 91, 1061, 1100 |
| Hin6I | GCGC | 5 cut(s) | 326, 695, 858, 975, 1381 |
| HinP1I | GCGC | 5 cut(s) | 326, 695, 858, 975, 1381 |
| HincII | GTYRAC | 3 cut(s) | 85, 1005, 1360 |
| HindII | GTYRAC | 3 cut(s) | 85, 1005, 1360 |
| HinfI | GANTC | 5 cut(s) | 98, 336, 477, 787, 918 |
| HpaI | GTTAAC | 1 cut(s) | 1005 |
| HphI | GGTGA | 5 cut(s) | 145, 193, 353, 391, 1403 |
| Hpy166II | GTNNAC | 4 cut(s) | 85, 208, 1005, 1360 |
| Hpy188I | TCNGA | 5 cut(s) | 270, 428, 436, 531, 904 |
| Hpy188III | TCNNGA | 7 cut(s) | 55, 67, 1097, 1247, 1603, 1658, 1717 |
| Hpy8I | GTNNAC | 4 cut(s) | 85, 208, 1005, 1360 |
| Hpy99I | CGWCG | 2 cut(s) | 610, 1718 |
| HpyAV | CCTTC | 8 cut(s) | 64, 724, 865, 1118, 1131, 1417, 1468, 1609 |
| HpyCH4III | ACNGT | 4 cut(s) | 391, 632, 1253, 1535 |
| HpyCH4IV | ACGT | 4 cut(s) | 144, 649, 852, 1007 |
| HpyCH4V | TGCA | 7 cut(s) | 7, 192, 1198, 1259, 1473, 1618, 1681 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 125, 429, 554, 932, 946, 1715 |
| HpyF3I | CTNAG | 8 cut(s) | 108, 129, 228, 405, 464, 510, 530, 698 |
| HpySE526I | ACGT | 4 cut(s) | 144, 649, 852, 1007 |
| Hsp92I | GRCGYC | 2 cut(s) | 144, 767 |
| Hsp92II | CATG | 3 cut(s) | 91, 1061, 1100 |
| HspAI | GCGC | 5 cut(s) | 326, 695, 858, 975, 1381 |
| Ksp22I | TGATCA | 2 cut(s) | 1093, 1654 |
| KspAI | GTTAAC | 1 cut(s) | 1005 |
| KspI | CCGCGG | 1 cut(s) | 1491 |
| Kzo9I | GATC | 7 cut(s) | 950, 969, 996, 1014, 1093, 1623, 1654 |
| LguI | GCTCTTC | 1 cut(s) | 1209 |
| LmnI | GCTCC | 9 cut(s) | 437, 602, 1048, 1068, 1267, 1303, 1411, 1438, 1610 |
| Lsp1109I | GCAGC | 3 cut(s) | 557, 1209, 1292 |
| LweI | GCATC | 5 cut(s) | 106, 524, 743, 1246, 1390 |
| MaeI | CTAG | 5 cut(s) | 639, 710, 782, 1452, 1732 |
| MaeII | ACGT | 4 cut(s) | 144, 649, 852, 1007 |
| MaeIII | GTNAC | 3 cut(s) | 768, 988, 1074 |
| MalI | GATC | 7 cut(s) | 952, 971, 998, 1016, 1095, 1625, 1656 |
| MbiI | CCGCTC | 1 cut(s) | 1219 |
| MboI | GATC | 7 cut(s) | 950, 969, 996, 1014, 1093, 1623, 1654 |
| MboII | GAAGA | 5 cut(s) | 87, 606, 766, 1226, 1553 |
| MfeI | CAATTG | 1 cut(s) | 663 |
| MflI | RGATCY | 1 cut(s) | 969 |
| MhlI | GDGCHC | 5 cut(s) | 371, 559, 746, 1057, 1065 |
| MluCI | AATT | 5 cut(s) | 585, 663, 1229, 1322, 1552 |
| MlyI | GAGTC | 5 cut(s) | 107, 345, 486, 796, 912 |
| MmeI | TCCRAC | 5 cut(s) | 882, 1128, 1245, 1389, 1596 |
| MseI | TTAA | 6 cut(s) | 300, 1004, 1172, 1286, 1418, 1578 |
| MslI | CAYNNNNRTG | 1 cut(s) | 739 |
| MspA1I | CMGCKG | 2 cut(s) | 548, 1490 |
| MspR9I | CCNGG | 4 cut(s) | 16, 739, 915, 1067 |
| MunI | CAATTG | 1 cut(s) | 663 |
| Mva1269I | GAATGC | 2 cut(s) | 1042, 1473 |
| MvaI | CCWGG | 4 cut(s) | 16, 739, 915, 1067 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 125, 429, 554, 932, 946, 1715 |
| NdeI | CATATG | 1 cut(s) | 959 |
| NdeII | GATC | 7 cut(s) | 950, 969, 996, 1014, 1093, 1623, 1654 |
| NlaIII | CATG | 3 cut(s) | 91, 1061, 1100 |
| NlaIV | GGNNCC | 8 cut(s) | 275, 370, 433, 556, 743, 833, 909, 1612 |
| NmeAIII | GCCGAG | 2 cut(s) | 841, 1112 |
| NmuCI | GTSAC | 2 cut(s) | 768, 988 |
| NruI | TCGCGA | 1 cut(s) | 1718 |
| NsbI | TGCGCA | 1 cut(s) | 976 |
| NspI | RCATGY | 1 cut(s) | 91 |
| PagI | TCATGA | 1 cut(s) | 1096 |
| PciSI | GCTCTTC | 1 cut(s) | 1209 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 150, 862 |
| PctI | GAATGC | 2 cut(s) | 1042, 1473 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 963 |
| PfoI | TCCNGGA | 1 cut(s) | 913 |
| PkrI | GCNGC | 8 cut(s) | 539, 547, 678, 1085, 1221, 1224, 1282, 1489 |
| Ple19I | CGATCG | 2 cut(s) | 953, 999 |
| PleI | GAGTC | 5 cut(s) | 106, 344, 485, 795, 912 |
| PpsI | GAGTC | 5 cut(s) | 106, 344, 485, 795, 912 |
| PpuMI | RGGWCCY | 2 cut(s) | 831, 907 |
| Psp124BI | GAGCTC | 1 cut(s) | 1065 |
| Psp5II | RGGWCCY | 2 cut(s) | 831, 907 |
| Psp6I | CCWGG | 4 cut(s) | 14, 737, 913, 1065 |
| PspFI | CCCAGC | 1 cut(s) | 92 |
| PspGI | CCWGG | 4 cut(s) | 14, 737, 913, 1065 |
| PspN4I | GGNNCC | 8 cut(s) | 275, 370, 433, 556, 743, 833, 909, 1612 |
| PspPI | GGNCC | 6 cut(s) | 706, 831, 907, 1201, 1485, 1669 |
| PspPPI | RGGWCCY | 2 cut(s) | 831, 907 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 257, 289 |
| PsuI | RGATCY | 1 cut(s) | 969 |
| PvuI | CGATCG | 2 cut(s) | 953, 999 |
| RgaI | GCGATCGC | 1 cut(s) | 999 |
| RruI | TCGCGA | 1 cut(s) | 1718 |
| RsaI | GTAC | 3 cut(s) | 488, 851, 1456 |
| RsaNI | GTAC | 3 cut(s) | 487, 850, 1455 |
| RseI | CAYNNNNRTG | 1 cut(s) | 739 |
| SacI | GAGCTC | 1 cut(s) | 1065 |
| SacII | CCGCGG | 1 cut(s) | 1491 |
| SalI | GTCGAC | 1 cut(s) | 83 |
| SapI | GCTCTTC | 1 cut(s) | 1209 |
| SaqAI | TTAA | 6 cut(s) | 300, 1004, 1172, 1286, 1418, 1578 |
| SatI | GCNGC | 8 cut(s) | 538, 546, 677, 1084, 1220, 1223, 1281, 1488 |
| Sau3AI | GATC | 7 cut(s) | 950, 969, 996, 1014, 1093, 1623, 1654 |
| Sau96I | GGNCC | 6 cut(s) | 706, 831, 907, 1201, 1485, 1669 |
| ScaI | AGTACT | 1 cut(s) | 488 |
| SchI | GAGTC | 5 cut(s) | 107, 345, 486, 796, 912 |
| ScrFI | CCNGG | 4 cut(s) | 16, 739, 915, 1067 |
| SduI | GDGCHC | 5 cut(s) | 371, 559, 746, 1057, 1065 |
| SfaAI | GCGATCGC | 1 cut(s) | 999 |
| SfaNI | GCATC | 5 cut(s) | 106, 524, 743, 1246, 1390 |
| Sfr303I | CCGCGG | 1 cut(s) | 1491 |
| SgfI | GCGATCGC | 1 cut(s) | 999 |
| SgrBI | CCGCGG | 1 cut(s) | 1491 |
| SinI | GGWCC | 4 cut(s) | 831, 907, 1201, 1669 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 739 |
| SmlI | CTYRAG | 2 cut(s) | 55, 397 |
| SmoI | CTYRAG | 2 cut(s) | 55, 397 |
| Sse9I | AATT | 5 cut(s) | 585, 663, 1229, 1322, 1552 |
| SspI | AATATT | 1 cut(s) | 1636 |
| SspMI | CTAG | 5 cut(s) | 639, 710, 782, 1452, 1732 |
| SstI | GAGCTC | 1 cut(s) | 1065 |
| StyD4I | CCNGG | 4 cut(s) | 14, 737, 913, 1065 |
| StyI | CCWWGG | 2 cut(s) | 559, 746 |
| TaaI | ACNGT | 4 cut(s) | 391, 632, 1253, 1535 |
| TaiI | ACGT | 4 cut(s) | 147, 652, 855, 1010 |
| TaqI | TCGA | 6 cut(s) | 84, 153, 517, 683, 810, 1604 |
| TasI | AATT | 5 cut(s) | 585, 663, 1229, 1322, 1552 |
| TatI | WGTACW | 1 cut(s) | 486 |
| TauI | GCSGC | 5 cut(s) | 540, 679, 1086, 1222, 1490 |
| Tru1I | TTAA | 6 cut(s) | 300, 1004, 1172, 1286, 1418, 1578 |
| Tru9I | TTAA | 6 cut(s) | 300, 1004, 1172, 1286, 1418, 1578 |
| TscAI | CASTG | 7 cut(s) | 363, 518, 629, 706, 1170, 1213, 1540 |
| TseFI | GTSAC | 2 cut(s) | 768, 988 |
| TseI | GCWGC | 3 cut(s) | 545, 1222, 1280 |
| Tsp45I | GTSAC | 2 cut(s) | 768, 988 |
| TspDTI | ATGAA | 4 cut(s) | 273, 743, 1466, 1565 |
| TspGWI | ACGGA | 1 cut(s) | 615 |
| TspRI | CASTG | 7 cut(s) | 363, 518, 629, 706, 1170, 1213, 1540 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 963 |
| VpaK11BI | GGWCC | 4 cut(s) | 831, 907, 1201, 1669 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 642 |
| XceI | RCATGY | 1 cut(s) | 91 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 491 |
| XmiI | GTMKAC | 2 cut(s) | 84, 207 |
| XspI | CTAG | 5 cut(s) | 639, 710, 782, 1452, 1732 |
| ZraI | GACGTC | 1 cut(s) | 145 |
| ZrmI | AGTACT | 1 cut(s) | 488 |