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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 449 |
| Acc36I | ACCTGC | 1 cut(s) | 449 |
| Acc65I | GGTACC | 1 cut(s) | 43 |
| AccB1I | GGYRCC | 1 cut(s) | 43 |
| AccBSI | CCGCTC | 2 cut(s) | 103, 484 |
| AccII | CGCG | 3 cut(s) | 150, 229, 384 |
| AciI | CCGC | 3 cut(s) | 103, 403, 484 |
| AclWI | GGATC | 1 cut(s) | 188 |
| AcuI | CTGAAG | 1 cut(s) | 101 |
| AfaI | GTAC | 1 cut(s) | 45 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 76, 139 |
| AgsI | TTSAA | 3 cut(s) | 88, 268, 455 |
| AjnI | CCWGG | 2 cut(s) | 132, 138 |
| AluBI | AGCT | 5 cut(s) | 8, 115, 324, 451, 528 |
| AluI | AGCT | 5 cut(s) | 8, 115, 324, 451, 528 |
| AlwI | GGATC | 1 cut(s) | 188 |
| Ama87I | CYCGRG | 1 cut(s) | 251 |
| AoxI | GGCC | 3 cut(s) | 136, 240, 248 |
| ApaI | GGGCCC | 1 cut(s) | 252 |
| Asp718I | GGTACC | 1 cut(s) | 43 |
| AspLEI | GCGC | 1 cut(s) | 229 |
| AspS9I | GGNCC | 4 cut(s) | 106, 136, 248, 249 |
| AsuC2I | CCSGG | 3 cut(s) | 70, 252, 253 |
| AsuHPI | GGTGA | 2 cut(s) | 224, 473 |
| AvaI | CYCGRG | 1 cut(s) | 251 |
| AvaII | GGWCC | 1 cut(s) | 106 |
| BaeGI | GKGCMC | 1 cut(s) | 252 |
| BanI | GGYRCC | 1 cut(s) | 43 |
| BanII | GRGCYC | 1 cut(s) | 252 |
| BccI | CCATC | 3 cut(s) | 170, 187, 472 |
| BciT130I | CCWGG | 2 cut(s) | 134, 140 |
| BcnI | CCSGG | 3 cut(s) | 70, 252, 253 |
| BfaI | CTAG | 1 cut(s) | 315 |
| BfmI | CTRYAG | 1 cut(s) | 277 |
| BfuAI | ACCTGC | 1 cut(s) | 449 |
| BisI | GCNGC | 1 cut(s) | 485 |
| BlsI | GCNGC | 1 cut(s) | 486 |
| Bme1390I | CCNGG | 5 cut(s) | 70, 134, 140, 252, 253 |
| Bme18I | GGWCC | 1 cut(s) | 106 |
| BmeT110I | CYCGRG | 1 cut(s) | 251 |
| BmgT120I | GGNCC | 4 cut(s) | 106, 136, 248, 249 |
| BmiI | GGNNCC | 6 cut(s) | 45, 107, 249, 250, 342, 369 |
| BmrFI | CCNGG | 5 cut(s) | 70, 134, 140, 252, 253 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 352, 384 |
| BpuMI | CCSGG | 3 cut(s) | 70, 252, 253 |
| BsaJI | CCNNGG | 3 cut(s) | 133, 243, 251 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 76, 139 |
| BseBI | CCWGG | 2 cut(s) | 134, 140 |
| BseDI | CCNNGG | 3 cut(s) | 133, 243, 251 |
| BseGI | GGATG | 2 cut(s) | 198, 448 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 76, 139 |
| BseMII | CTCAG | 1 cut(s) | 530 |
| BseSI | GKGCMC | 1 cut(s) | 252 |
| Bsh1236I | CGCG | 3 cut(s) | 150, 229, 384 |
| BshFI | GGCC | 3 cut(s) | 138, 242, 250 |
| BshNI | GGYRCC | 1 cut(s) | 43 |
| BsiHKCI | CYCGRG | 1 cut(s) | 251 |
| BsiSI | CCGG | 2 cut(s) | 70, 252 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 76, 139 |
| BsnI | GGCC | 3 cut(s) | 138, 242, 250 |
| BsoBI | CYCGRG | 1 cut(s) | 251 |
| Bsp120I | GGGCCC | 1 cut(s) | 248 |
| Bsp1286I | GDGCHC | 1 cut(s) | 252 |
| Bsp143I | GATC | 4 cut(s) | 55, 180, 206, 379 |
| BspACI | CCGC | 3 cut(s) | 103, 403, 484 |
| BspANI | GGCC | 3 cut(s) | 138, 242, 250 |
| BspCNI | CTCAG | 1 cut(s) | 529 |
| BspFNI | CGCG | 3 cut(s) | 150, 229, 384 |
| BspHI | TCATGA | 1 cut(s) | 183 |
| BspLI | GGNNCC | 6 cut(s) | 45, 107, 249, 250, 342, 369 |
| BspMAI | CTGCAG | 1 cut(s) | 281 |
| BspMI | ACCTGC | 1 cut(s) | 449 |
| BspPI | GGATC | 1 cut(s) | 188 |
| BspQI | GCTCTTC | 1 cut(s) | 263 |
| BspT107I | GGYRCC | 1 cut(s) | 43 |
| BsrBI | CCGCTC | 2 cut(s) | 103, 484 |
| BssECI | CCNNGG | 3 cut(s) | 133, 243, 251 |
| BssMI | GATC | 4 cut(s) | 55, 180, 206, 379 |
| Bst2UI | CCWGG | 2 cut(s) | 134, 140 |
| Bst4CI | ACNGT | 2 cut(s) | 217, 434 |
| Bst6I | CTCTTC | 1 cut(s) | 263 |
| BstC8I | GCNNGC | 2 cut(s) | 6, 165 |
| BstDEI | CTNAG | 3 cut(s) | 355, 516, 534 |
| BstF5I | GGATG | 2 cut(s) | 198, 448 |
| BstFNI | CGCG | 3 cut(s) | 150, 229, 384 |
| BstHHI | GCGC | 1 cut(s) | 229 |
| BstKTI | GATC | 4 cut(s) | 58, 183, 209, 382 |
| BstMBI | GATC | 4 cut(s) | 55, 180, 206, 379 |
| BstNI | CCWGG | 2 cut(s) | 134, 140 |
| BstSCI | CCNGG | 5 cut(s) | 68, 132, 138, 250, 251 |
| BstSFI | CTRYAG | 1 cut(s) | 277 |
| BstSLI | GKGCMC | 1 cut(s) | 252 |
| BstUI | CGCG | 3 cut(s) | 150, 229, 384 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 32 |
| BsuRI | GGCC | 3 cut(s) | 138, 242, 250 |
| BtsCI | GGATG | 2 cut(s) | 198, 448 |
| BtsIMutI | CAGTG | 1 cut(s) | 222 |
| BveI | ACCTGC | 1 cut(s) | 449 |
| Cac8I | GCNNGC | 2 cut(s) | 6, 165 |
| CciI | TCATGA | 1 cut(s) | 183 |
| CfoI | GCGC | 1 cut(s) | 229 |
| Cfr13I | GGNCC | 4 cut(s) | 106, 136, 248, 249 |
| Cfr9I | CCCGGG | 1 cut(s) | 251 |
| CseI | GACGC | 2 cut(s) | 218, 262 |
| Csp6I | GTAC | 1 cut(s) | 44 |
| CviAII | CATG | 3 cut(s) | 16, 26, 184 |
| CviQI | GTAC | 1 cut(s) | 44 |
| DdeI | CTNAG | 3 cut(s) | 355, 516, 534 |
| DpnI | GATC | 4 cut(s) | 57, 182, 208, 381 |
| DpnII | GATC | 4 cut(s) | 55, 180, 206, 379 |
| Eam1104I | CTCTTC | 1 cut(s) | 263 |
| EarI | CTCTTC | 1 cut(s) | 263 |
| Eco24I | GRGCYC | 1 cut(s) | 252 |
| Eco47I | GGWCC | 1 cut(s) | 106 |
| Eco57I | CTGAAG | 1 cut(s) | 101 |
| Eco88I | CYCGRG | 1 cut(s) | 251 |
| EcoO109I | RGGNCCY | 1 cut(s) | 248 |
| EcoRII | CCWGG | 2 cut(s) | 132, 138 |
| EcoT38I | GRGCYC | 1 cut(s) | 252 |
| FaeI | CATG | 3 cut(s) | 19, 29, 187 |
| FaiI | YATR | 6 cut(s) | 17, 27, 158, 185, 373, 439 |
| FatI | CATG | 3 cut(s) | 15, 25, 183 |
| FauI | CCCGC | 2 cut(s) | 96, 396 |
| Fnu4HI | GCNGC | 1 cut(s) | 485 |
| FokI | GGATG | 2 cut(s) | 205, 455 |
| FriOI | GRGCYC | 1 cut(s) | 252 |
| Fsp4HI | GCNGC | 1 cut(s) | 485 |
| FspBI | CTAG | 1 cut(s) | 315 |
| GlaI | GCGC | 1 cut(s) | 228 |
| GluI | GCNGC | 1 cut(s) | 485 |
| HaeIII | GGCC | 3 cut(s) | 138, 242, 250 |
| HapII | CCGG | 2 cut(s) | 70, 252 |
| HgaI | GACGC | 2 cut(s) | 218, 262 |
| HhaI | GCGC | 1 cut(s) | 229 |
| Hin1II | CATG | 3 cut(s) | 19, 29, 187 |
| Hin6I | GCGC | 1 cut(s) | 227 |
| HinP1I | GCGC | 1 cut(s) | 227 |
| HincII | GTYRAC | 2 cut(s) | 430, 501 |
| HindII | GTYRAC | 2 cut(s) | 430, 501 |
| HpaI | GTTAAC | 1 cut(s) | 501 |
| HpaII | CCGG | 2 cut(s) | 70, 252 |
| HphI | GGTGA | 2 cut(s) | 224, 473 |
| Hpy166II | GTNNAC | 3 cut(s) | 13, 430, 501 |
| Hpy188I | TCNGA | 2 cut(s) | 120, 301 |
| Hpy188III | TCNNGA | 4 cut(s) | 59, 94, 184, 377 |
| Hpy8I | GTNNAC | 3 cut(s) | 13, 430, 501 |
| HpyAV | CCTTC | 2 cut(s) | 183, 487 |
| HpyCH4III | ACNGT | 2 cut(s) | 217, 434 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 279, 418 |
| HpyF3I | CTNAG | 3 cut(s) | 355, 516, 534 |
| Hsp92II | CATG | 3 cut(s) | 19, 29, 187 |
| HspAI | GCGC | 1 cut(s) | 227 |
| KpnI | GGTACC | 1 cut(s) | 47 |
| KspAI | GTTAAC | 1 cut(s) | 501 |
| Kzo9I | GATC | 4 cut(s) | 55, 180, 206, 379 |
| LguI | GCTCTTC | 1 cut(s) | 263 |
| LmnI | GCTCC | 4 cut(s) | 100, 321, 373, 481 |
| MaeI | CTAG | 1 cut(s) | 315 |
| MaeIII | GTNAC | 2 cut(s) | 72, 230 |
| MalI | GATC | 4 cut(s) | 57, 182, 208, 381 |
| MbiI | CCGCTC | 2 cut(s) | 103, 484 |
| MboI | GATC | 4 cut(s) | 55, 180, 206, 379 |
| MboII | GAAGA | 1 cut(s) | 280 |
| MfeI | CAATTG | 1 cut(s) | 168 |
| MhlI | GDGCHC | 1 cut(s) | 252 |
| MluCI | AATT | 2 cut(s) | 19, 168 |
| MmeI | TCCRAC | 1 cut(s) | 299 |
| MseI | TTAA | 1 cut(s) | 500 |
| MspI | CCGG | 2 cut(s) | 70, 252 |
| MspR9I | CCNGG | 5 cut(s) | 70, 134, 140, 252, 253 |
| MunI | CAATTG | 1 cut(s) | 168 |
| MvaI | CCWGG | 2 cut(s) | 134, 140 |
| MvnI | CGCG | 3 cut(s) | 150, 229, 384 |
| NciI | CCSGG | 3 cut(s) | 70, 252, 253 |
| NdeII | GATC | 4 cut(s) | 55, 180, 206, 379 |
| NlaIII | CATG | 3 cut(s) | 19, 29, 187 |
| NlaIV | GGNNCC | 6 cut(s) | 45, 107, 249, 250, 342, 369 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| NmuCI | GTSAC | 1 cut(s) | 230 |
| PagI | TCATGA | 1 cut(s) | 183 |
| PaqCI | CACCTGC | 1 cut(s) | 449 |
| PciSI | GCTCTTC | 1 cut(s) | 263 |
| PkrI | GCNGC | 1 cut(s) | 486 |
| Psp6I | CCWGG | 2 cut(s) | 132, 138 |
| PspGI | CCWGG | 2 cut(s) | 132, 138 |
| PspN4I | GGNNCC | 6 cut(s) | 45, 107, 249, 250, 342, 369 |
| PspOMI | GGGCCC | 1 cut(s) | 248 |
| PspPI | GGNCC | 4 cut(s) | 106, 136, 248, 249 |
| PsrI | GAACNNNNNNTAC | 1 cut(s) | 37 |
| PstI | CTGCAG | 1 cut(s) | 281 |
| RsaI | GTAC | 1 cut(s) | 45 |
| RsaNI | GTAC | 1 cut(s) | 44 |
| SapI | GCTCTTC | 1 cut(s) | 263 |
| SaqAI | TTAA | 1 cut(s) | 500 |
| SatI | GCNGC | 1 cut(s) | 485 |
| Sau3AI | GATC | 4 cut(s) | 55, 180, 206, 379 |
| Sau96I | GGNCC | 4 cut(s) | 106, 136, 248, 249 |
| ScrFI | CCNGG | 5 cut(s) | 70, 134, 140, 252, 253 |
| SduI | GDGCHC | 1 cut(s) | 252 |
| SfcI | CTRYAG | 1 cut(s) | 277 |
| SinI | GGWCC | 1 cut(s) | 106 |
| SmaI | CCCGGG | 1 cut(s) | 253 |
| Sse9I | AATT | 2 cut(s) | 19, 168 |
| SsiI | CCGC | 3 cut(s) | 103, 403, 484 |
| SspMI | CTAG | 1 cut(s) | 315 |
| StyD4I | CCNGG | 5 cut(s) | 68, 132, 138, 250, 251 |
| TaaI | ACNGT | 2 cut(s) | 217, 434 |
| TaqI | TCGA | 1 cut(s) | 521 |
| TasI | AATT | 2 cut(s) | 19, 168 |
| TauI | GCSGC | 1 cut(s) | 487 |
| Tru1I | TTAA | 1 cut(s) | 500 |
| Tru9I | TTAA | 1 cut(s) | 500 |
| TscAI | CASTG | 1 cut(s) | 222 |
| TseFI | GTSAC | 1 cut(s) | 230 |
| Tsp45I | GTSAC | 1 cut(s) | 230 |
| TspDTI | ATGAA | 1 cut(s) | 32 |
| TspGWI | ACGGA | 1 cut(s) | 98 |
| TspMI | CCCGGG | 1 cut(s) | 251 |
| TspRI | CASTG | 1 cut(s) | 222 |
| VpaK11BI | GGWCC | 1 cut(s) | 106 |
| XmaI | CCCGGG | 1 cut(s) | 251 |
| XspI | CTAG | 1 cut(s) | 315 |