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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 172 |
| AccBSI | CCGCTC | 1 cut(s) | 730 |
| AccI | GTMKAC | 1 cut(s) | 682 |
| AccII | CGCG | 2 cut(s) | 523, 551 |
| AciI | CCGC | 8 cut(s) | 64, 105, 140, 251, 606, 650, 730, 770 |
| AclWI | GGATC | 1 cut(s) | 783 |
| AcvI | CACGTG | 1 cut(s) | 488 |
| AcyI | GRCGYC | 1 cut(s) | 169 |
| AfaI | GTAC | 2 cut(s) | 406, 842 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 317, 351, 467 |
| AflIII | ACRYGT | 2 cut(s) | 393, 485 |
| AgsI | TTSAA | 1 cut(s) | 58 |
| AhlI | ACTAGT | 1 cut(s) | 536 |
| AjnI | CCWGG | 1 cut(s) | 264 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 174, 206 |
| AluBI | AGCT | 5 cut(s) | 80, 152, 445, 662, 697 |
| AluI | AGCT | 5 cut(s) | 80, 152, 445, 662, 697 |
| Alw21I | GWGCWC | 1 cut(s) | 112 |
| Alw26I | GTCTC | 4 cut(s) | 272, 341, 507, 574 |
| AlwI | GGATC | 1 cut(s) | 783 |
| Ama87I | CYCGRG | 7 cut(s) | 111, 123, 381, 452, 526, 613, 666 |
| AoxI | GGCC | 4 cut(s) | 137, 435, 616, 744 |
| ApaI | GGGCCC | 1 cut(s) | 620 |
| ApeKI | GCWGC | 1 cut(s) | 442 |
| AspLEI | GCGC | 1 cut(s) | 525 |
| AspS9I | GGNCC | 3 cut(s) | 354, 616, 617 |
| AsuC2I | CCSGG | 4 cut(s) | 614, 615, 667, 668 |
| AsuHPI | GGTGA | 3 cut(s) | 238, 268, 564 |
| AvaI | CYCGRG | 7 cut(s) | 111, 123, 381, 452, 526, 613, 666 |
| AvaII | GGWCC | 1 cut(s) | 354 |
| BaeGI | GKGCMC | 2 cut(s) | 403, 620 |
| BanII | GRGCYC | 2 cut(s) | 382, 620 |
| BbrPI | CACGTG | 1 cut(s) | 488 |
| BbsI | GAAGAC | 1 cut(s) | 243 |
| Bbv12I | GWGCWC | 1 cut(s) | 112 |
| BbvCI | CCTCAGC | 1 cut(s) | 87 |
| BbvI | GCAGC | 1 cut(s) | 454 |
| BccI | CCATC | 7 cut(s) | 356, 404, 427, 651, 718, 848, 883 |
| BciT130I | CCWGG | 1 cut(s) | 266 |
| BciVI | GTATCC | 1 cut(s) | 823 |
| BcnI | CCSGG | 4 cut(s) | 614, 615, 667, 668 |
| BcoDI | GTCTC | 4 cut(s) | 272, 341, 507, 574 |
| BcuI | ACTAGT | 1 cut(s) | 536 |
| BfaI | CTAG | 5 cut(s) | 47, 153, 162, 261, 537 |
| BfmI | CTRYAG | 1 cut(s) | 312 |
| BfuI | GTATCC | 1 cut(s) | 823 |
| BglII | AGATCT | 1 cut(s) | 530 |
| BisI | GCNGC | 3 cut(s) | 140, 443, 731 |
| BlsI | GCNGC | 3 cut(s) | 141, 444, 732 |
| Bme1390I | CCNGG | 5 cut(s) | 266, 614, 615, 667, 668 |
| Bme18I | GGWCC | 1 cut(s) | 354 |
| BmeT110I | CYCGRG | 7 cut(s) | 111, 123, 381, 452, 526, 613, 666 |
| BmgT120I | GGNCC | 3 cut(s) | 354, 616, 617 |
| BmiI | GGNNCC | 5 cut(s) | 117, 355, 356, 379, 618 |
| BmrFI | CCNGG | 5 cut(s) | 266, 614, 615, 667, 668 |
| BmrI | ACTGGG | 1 cut(s) | 458 |
| BmsI | GCATC | 2 cut(s) | 28, 118 |
| BmuI | ACTGGG | 1 cut(s) | 458 |
| BpiI | GAAGAC | 1 cut(s) | 243 |
| Bpu10I | CCTNAGC | 3 cut(s) | 87, 306, 734 |
| BpuEI | CTTGAG | 1 cut(s) | 719 |
| BpuMI | CCSGG | 4 cut(s) | 614, 615, 667, 668 |
| BsaAI | YACGTR | 1 cut(s) | 488 |
| BsaBI | GATNNNNATC | 2 cut(s) | 632, 780 |
| BsaHI | GRCGYC | 1 cut(s) | 169 |
| BsaI | GGTCTC | 2 cut(s) | 507, 574 |
| BsaJI | CCNNGG | 3 cut(s) | 451, 613, 666 |
| BsaWI | WCCGGW | 1 cut(s) | 786 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 369, 399 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 317, 351, 467 |
| Bse1I | ACTGG | 3 cut(s) | 183, 464, 747 |
| Bse8I | GATNNNNATC | 2 cut(s) | 632, 780 |
| BseBI | CCWGG | 1 cut(s) | 266 |
| BseDI | CCNNGG | 3 cut(s) | 451, 613, 666 |
| BseGI | GGATG | 3 cut(s) | 438, 643, 760 |
| BseJI | GATNNNNATC | 2 cut(s) | 632, 780 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 317, 351, 467 |
| BseMII | CTCAG | 5 cut(s) | 17, 60, 78, 297, 748 |
| BseNI | ACTGG | 3 cut(s) | 183, 464, 747 |
| BseSI | GKGCMC | 2 cut(s) | 403, 620 |
| BseXI | GCAGC | 1 cut(s) | 454 |
| BseYI | CCCAGC | 1 cut(s) | 866 |
| Bsh1236I | CGCG | 2 cut(s) | 523, 551 |
| BshFI | GGCC | 4 cut(s) | 139, 437, 618, 746 |
| BsiHKAI | GWGCWC | 1 cut(s) | 112 |
| BsiHKCI | CYCGRG | 7 cut(s) | 111, 123, 381, 452, 526, 613, 666 |
| BsiSI | CCGG | 3 cut(s) | 614, 667, 787 |
| BslFI | GGGAC | 3 cut(s) | 156, 367, 444 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 317, 351, 467 |
| BsmAI | GTCTC | 4 cut(s) | 272, 341, 507, 574 |
| BsmFI | GGGAC | 3 cut(s) | 156, 367, 444 |
| BsnI | GGCC | 4 cut(s) | 139, 437, 618, 746 |
| Bso31I | GGTCTC | 2 cut(s) | 507, 574 |
| BsoBI | CYCGRG | 7 cut(s) | 111, 123, 381, 452, 526, 613, 666 |
| Bsp120I | GGGCCC | 1 cut(s) | 616 |
| Bsp1286I | GDGCHC | 4 cut(s) | 112, 382, 403, 620 |
| Bsp143I | GATC | 3 cut(s) | 530, 627, 775 |
| Bsp68I | TCGCGA | 1 cut(s) | 551 |
| BspACI | CCGC | 8 cut(s) | 64, 105, 140, 251, 606, 650, 730, 770 |
| BspANI | GGCC | 4 cut(s) | 139, 437, 618, 746 |
| BspCNI | CTCAG | 5 cut(s) | 18, 61, 79, 298, 747 |
| BspFNI | CGCG | 2 cut(s) | 523, 551 |
| BspLI | GGNNCC | 5 cut(s) | 117, 355, 356, 379, 618 |
| BspMAI | CTGCAG | 1 cut(s) | 316 |
| BspPI | GGATC | 1 cut(s) | 783 |
| BspTNI | GGTCTC | 2 cut(s) | 507, 574 |
| BsrBI | CCGCTC | 1 cut(s) | 730 |
| BsrI | ACTGG | 3 cut(s) | 183, 464, 747 |
| BssECI | CCNNGG | 3 cut(s) | 451, 613, 666 |
| BssMI | GATC | 3 cut(s) | 530, 627, 775 |
| BssNI | GRCGYC | 1 cut(s) | 169 |
| Bst2UI | CCWGG | 1 cut(s) | 266 |
| Bst4CI | ACNGT | 4 cut(s) | 242, 292, 680, 835 |
| BstACI | GRCGYC | 1 cut(s) | 169 |
| BstBAI | YACGTR | 1 cut(s) | 488 |
| BstC8I | GCNNGC | 3 cut(s) | 150, 312, 706 |
| BstDEI | CTNAG | 6 cut(s) | 26, 69, 87, 306, 637, 734 |
| BstF5I | GGATG | 3 cut(s) | 438, 643, 760 |
| BstFNI | CGCG | 2 cut(s) | 523, 551 |
| BstHHI | GCGC | 1 cut(s) | 525 |
| BstKTI | GATC | 3 cut(s) | 533, 630, 778 |
| BstMAI | GTCTC | 4 cut(s) | 272, 341, 507, 574 |
| BstMBI | GATC | 3 cut(s) | 530, 627, 775 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 145, 158, 307, 767 |
| BstNI | CCWGG | 1 cut(s) | 266 |
| BstNSI | RCATGY | 1 cut(s) | 397 |
| BstSCI | CCNGG | 5 cut(s) | 264, 612, 613, 665, 666 |
| BstSFI | CTRYAG | 1 cut(s) | 312 |
| BstSLI | GKGCMC | 2 cut(s) | 403, 620 |
| BstUI | CGCG | 2 cut(s) | 523, 551 |
| BstV1I | GCAGC | 1 cut(s) | 454 |
| BstV2I | GAAGAC | 1 cut(s) | 243 |
| BstX2I | RGATCY | 2 cut(s) | 530, 775 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 272 |
| BstYI | RGATCY | 2 cut(s) | 530, 775 |
| BsuI | GTATCC | 1 cut(s) | 823 |
| BsuRI | GGCC | 4 cut(s) | 139, 437, 618, 746 |
| BtgZI | GCGATG | 1 cut(s) | 566 |
| BtsCI | GGATG | 3 cut(s) | 438, 643, 760 |
| BtsI | GCAGTG | 1 cut(s) | 861 |
| BtsIMutI | CAGTG | 2 cut(s) | 247, 861 |
| BtuMI | TCGCGA | 1 cut(s) | 551 |
| Cac8I | GCNNGC | 3 cut(s) | 150, 312, 706 |
| CfoI | GCGC | 1 cut(s) | 525 |
| Cfr13I | GGNCC | 3 cut(s) | 354, 616, 617 |
| Cfr9I | CCCGGG | 2 cut(s) | 613, 666 |
| Csp6I | GTAC | 2 cut(s) | 405, 841 |
| CviAII | CATG | 4 cut(s) | 95, 145, 359, 394 |
| CviQI | GTAC | 2 cut(s) | 405, 841 |
| DdeI | CTNAG | 6 cut(s) | 26, 69, 87, 306, 637, 734 |
| DpnI | GATC | 3 cut(s) | 532, 629, 777 |
| DpnII | GATC | 3 cut(s) | 530, 627, 775 |
| EciI | GGCGGA | 2 cut(s) | 266, 639 |
| Eco24I | GRGCYC | 2 cut(s) | 382, 620 |
| Eco31I | GGTCTC | 2 cut(s) | 507, 574 |
| Eco47I | GGWCC | 1 cut(s) | 354 |
| Eco72I | CACGTG | 1 cut(s) | 488 |
| Eco88I | CYCGRG | 7 cut(s) | 111, 123, 381, 452, 526, 613, 666 |
| EcoO109I | RGGNCCY | 2 cut(s) | 354, 617 |
| EcoRII | CCWGG | 1 cut(s) | 264 |
| EcoT38I | GRGCYC | 2 cut(s) | 382, 620 |
| FaeI | CATG | 4 cut(s) | 98, 148, 362, 397 |
| FaiI | YATR | 6 cut(s) | 96, 146, 323, 360, 395, 902 |
| FaqI | GGGAC | 3 cut(s) | 156, 367, 444 |
| FatI | CATG | 4 cut(s) | 94, 144, 358, 393 |
| FauI | CCCGC | 1 cut(s) | 613 |
| FblI | GTMKAC | 1 cut(s) | 682 |
| Fnu4HI | GCNGC | 3 cut(s) | 140, 443, 731 |
| FokI | GGATG | 3 cut(s) | 445, 630, 767 |
| FriOI | GRGCYC | 2 cut(s) | 382, 620 |
| Fsp4HI | GCNGC | 3 cut(s) | 140, 443, 731 |
| FspBI | CTAG | 5 cut(s) | 47, 153, 162, 261, 537 |
| GlaI | GCGC | 1 cut(s) | 524 |
| GluI | GCNGC | 3 cut(s) | 140, 443, 731 |
| GsaI | CCCAGC | 1 cut(s) | 870 |
| HaeIII | GGCC | 4 cut(s) | 139, 437, 618, 746 |
| HapII | CCGG | 3 cut(s) | 614, 667, 787 |
| HhaI | GCGC | 1 cut(s) | 525 |
| Hin1I | GRCGYC | 1 cut(s) | 169 |
| Hin1II | CATG | 4 cut(s) | 98, 148, 362, 397 |
| Hin6I | GCGC | 1 cut(s) | 523 |
| HinP1I | GCGC | 1 cut(s) | 523 |
| HincII | GTYRAC | 1 cut(s) | 676 |
| HindII | GTYRAC | 1 cut(s) | 676 |
| HinfI | GANTC | 4 cut(s) | 121, 457, 633, 858 |
| HpaII | CCGG | 3 cut(s) | 614, 667, 787 |
| HphI | GGTGA | 3 cut(s) | 238, 268, 564 |
| Hpy166II | GTNNAC | 3 cut(s) | 167, 676, 683 |
| Hpy188I | TCNGA | 3 cut(s) | 27, 288, 632 |
| Hpy188III | TCNNGA | 8 cut(s) | 35, 113, 125, 454, 528, 550, 736, 752 |
| Hpy8I | GTNNAC | 3 cut(s) | 167, 676, 683 |
| HpyAV | CCTTC | 2 cut(s) | 706, 832 |
| HpyCH4III | ACNGT | 4 cut(s) | 242, 292, 680, 835 |
| HpyCH4IV | ACGT | 2 cut(s) | 169, 487 |
| HpyCH4V | TGCA | 5 cut(s) | 148, 220, 301, 314, 589 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 145, 158, 307, 767 |
| HpyF3I | CTNAG | 6 cut(s) | 26, 69, 87, 306, 637, 734 |
| HpySE526I | ACGT | 2 cut(s) | 169, 487 |
| Hsp92I | GRCGYC | 1 cut(s) | 169 |
| Hsp92II | CATG | 4 cut(s) | 98, 148, 362, 397 |
| HspAI | GCGC | 1 cut(s) | 523 |
| KflI | GGGWCCC | 1 cut(s) | 354 |
| Kzo9I | GATC | 3 cut(s) | 530, 627, 775 |
| LmnI | GCTCC | 3 cut(s) | 115, 377, 727 |
| Lsp1109I | GCAGC | 1 cut(s) | 454 |
| LweI | GCATC | 2 cut(s) | 28, 118 |
| MaeI | CTAG | 5 cut(s) | 47, 153, 162, 261, 537 |
| MaeII | ACGT | 2 cut(s) | 169, 487 |
| MaeIII | GTNAC | 4 cut(s) | 3, 256, 340, 655 |
| MalI | GATC | 3 cut(s) | 532, 629, 777 |
| MbiI | CCGCTC | 1 cut(s) | 730 |
| MboI | GATC | 3 cut(s) | 530, 627, 775 |
| MboII | GAAGA | 4 cut(s) | 86, 248, 820, 898 |
| MflI | RGATCY | 2 cut(s) | 530, 775 |
| MhlI | GDGCHC | 4 cut(s) | 112, 382, 403, 620 |
| MlyI | GAGTC | 2 cut(s) | 115, 466 |
| MseI | TTAA | 1 cut(s) | 600 |
| MslI | CAYNNNNRTG | 1 cut(s) | 99 |
| MspA1I | CMGCKG | 1 cut(s) | 64 |
| MspI | CCGG | 3 cut(s) | 614, 667, 787 |
| MspR9I | CCNGG | 5 cut(s) | 266, 614, 615, 667, 668 |
| MvaI | CCWGG | 1 cut(s) | 266 |
| MvnI | CGCG | 2 cut(s) | 523, 551 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 145, 158, 307, 767 |
| NciI | CCSGG | 4 cut(s) | 614, 615, 667, 668 |
| NdeII | GATC | 3 cut(s) | 530, 627, 775 |
| NlaIII | CATG | 4 cut(s) | 98, 148, 362, 397 |
| NlaIV | GGNNCC | 5 cut(s) | 117, 355, 356, 379, 618 |
| NmuCI | GTSAC | 1 cut(s) | 256 |
| NruI | TCGCGA | 1 cut(s) | 551 |
| NspI | RCATGY | 1 cut(s) | 397 |
| PaeR7I | CTCGAG | 2 cut(s) | 123, 526 |
| PciI | ACATGT | 1 cut(s) | 393 |
| PfeI | GAWTC | 2 cut(s) | 633, 858 |
| PkrI | GCNGC | 3 cut(s) | 141, 444, 732 |
| PleI | GAGTC | 2 cut(s) | 115, 465 |
| PmaCI | CACGTG | 1 cut(s) | 488 |
| PmlI | CACGTG | 1 cut(s) | 488 |
| PpsI | GAGTC | 2 cut(s) | 115, 465 |
| Ppu21I | YACGTR | 1 cut(s) | 488 |
| PpuMI | RGGWCCY | 1 cut(s) | 354 |
| PscI | ACATGT | 1 cut(s) | 393 |
| Psp5II | RGGWCCY | 1 cut(s) | 354 |
| Psp6I | CCWGG | 1 cut(s) | 264 |
| PspCI | CACGTG | 1 cut(s) | 488 |
| PspFI | CCCAGC | 1 cut(s) | 866 |
| PspGI | CCWGG | 1 cut(s) | 264 |
| PspN4I | GGNNCC | 5 cut(s) | 117, 355, 356, 379, 618 |
| PspOMI | GGGCCC | 1 cut(s) | 616 |
| PspPI | GGNCC | 3 cut(s) | 354, 616, 617 |
| PspPPI | RGGWCCY | 1 cut(s) | 354 |
| PstI | CTGCAG | 1 cut(s) | 316 |
| PsuI | RGATCY | 2 cut(s) | 530, 775 |
| RruI | TCGCGA | 1 cut(s) | 551 |
| RsaI | GTAC | 2 cut(s) | 406, 842 |
| RsaNI | GTAC | 2 cut(s) | 405, 841 |
| RseI | CAYNNNNRTG | 1 cut(s) | 99 |
| SaqAI | TTAA | 1 cut(s) | 600 |
| SatI | GCNGC | 3 cut(s) | 140, 443, 731 |
| Sau3AI | GATC | 3 cut(s) | 530, 627, 775 |
| Sau96I | GGNCC | 3 cut(s) | 354, 616, 617 |
| SchI | GAGTC | 2 cut(s) | 115, 466 |
| ScrFI | CCNGG | 5 cut(s) | 266, 614, 615, 667, 668 |
| SduI | GDGCHC | 4 cut(s) | 112, 382, 403, 620 |
| SfaNI | GCATC | 2 cut(s) | 28, 118 |
| SfcI | CTRYAG | 1 cut(s) | 312 |
| Sfr274I | CTCGAG | 2 cut(s) | 123, 526 |
| SinI | GGWCC | 1 cut(s) | 354 |
| SlaI | CTCGAG | 2 cut(s) | 123, 526 |
| SmaI | CCCGGG | 2 cut(s) | 615, 668 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 99 |
| SmlI | CTYRAG | 3 cut(s) | 123, 526, 698 |
| SmoI | CTYRAG | 3 cut(s) | 123, 526, 698 |
| SpeI | ACTAGT | 1 cut(s) | 536 |
| SsiI | CCGC | 8 cut(s) | 64, 105, 140, 251, 606, 650, 730, 770 |
| SspMI | CTAG | 5 cut(s) | 47, 153, 162, 261, 537 |
| StyD4I | CCNGG | 5 cut(s) | 264, 612, 613, 665, 666 |
| TaaI | ACNGT | 4 cut(s) | 242, 292, 680, 835 |
| TaiI | ACGT | 2 cut(s) | 172, 490 |
| TaqI | TCGA | 4 cut(s) | 124, 422, 527, 845 |
| TatI | WGTACW | 1 cut(s) | 840 |
| TauI | GCSGC | 2 cut(s) | 142, 733 |
| TfiI | GAWTC | 2 cut(s) | 633, 858 |
| Tru1I | TTAA | 1 cut(s) | 600 |
| Tru9I | TTAA | 1 cut(s) | 600 |
| TscAI | CASTG | 2 cut(s) | 247, 868 |
| TseFI | GTSAC | 1 cut(s) | 256 |
| TseI | GCWGC | 1 cut(s) | 442 |
| Tsp45I | GTSAC | 1 cut(s) | 256 |
| TspDTI | ATGAA | 1 cut(s) | 827 |
| TspMI | CCCGGG | 2 cut(s) | 613, 666 |
| TspRI | CASTG | 2 cut(s) | 247, 868 |
| VpaK11BI | GGWCC | 1 cut(s) | 354 |
| XceI | RCATGY | 1 cut(s) | 397 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 754 |
| XhoI | CTCGAG | 2 cut(s) | 123, 526 |
| XmaI | CCCGGG | 2 cut(s) | 613, 666 |
| XmiI | GTMKAC | 1 cut(s) | 682 |
| XspI | CTAG | 5 cut(s) | 47, 153, 162, 261, 537 |
| ZraI | GACGTC | 1 cut(s) | 170 |