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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 150 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 220 |
| AccII | CGCG | 2 cut(s) | 88, 381 |
| AciI | CCGC | 5 cut(s) | 88, 122, 192, 225, 348 |
| AcoI | YGGCCR | 1 cut(s) | 222 |
| AcyI | GRCGYC | 1 cut(s) | 147 |
| AfaI | GTAC | 1 cut(s) | 268 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 17, 104, 220, 250, 285, 350 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 99, 131 |
| AluBI | AGCT | 1 cut(s) | 61 |
| AluI | AGCT | 1 cut(s) | 61 |
| Alw21I | GWGCWC | 1 cut(s) | 265 |
| Ama87I | CYCGRG | 2 cut(s) | 243, 384 |
| AoxI | GGCC | 2 cut(s) | 222, 240 |
| ApaI | GGGCCC | 1 cut(s) | 244 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 119, 151 |
| AspLEI | GCGC | 3 cut(s) | 88, 381, 383 |
| AspS9I | GGNCC | 3 cut(s) | 155, 240, 241 |
| AsuC2I | CCSGG | 4 cut(s) | 17, 99, 280, 323 |
| AsuHPI | GGTGA | 2 cut(s) | 87, 337 |
| AvaI | CYCGRG | 2 cut(s) | 243, 384 |
| AvaII | GGWCC | 1 cut(s) | 155 |
| BaeGI | GKGCMC | 1 cut(s) | 244 |
| BanII | GRGCYC | 1 cut(s) | 244 |
| Bbv12I | GWGCWC | 1 cut(s) | 265 |
| BccI | CCATC | 2 cut(s) | 15, 230 |
| BceAI | ACGGC | 1 cut(s) | 82 |
| BciVI | GTATCC | 1 cut(s) | 247 |
| BcnI | CCSGG | 4 cut(s) | 17, 99, 280, 323 |
| BfaI | CTAG | 3 cut(s) | 363, 416, 439 |
| BfuI | GTATCC | 1 cut(s) | 247 |
| BglII | AGATCT | 1 cut(s) | 32 |
| BisI | GCNGC | 1 cut(s) | 225 |
| BlsI | GCNGC | 1 cut(s) | 226 |
| Bme1390I | CCNGG | 4 cut(s) | 17, 99, 280, 323 |
| Bme18I | GGWCC | 1 cut(s) | 155 |
| BmeT110I | CYCGRG | 2 cut(s) | 243, 384 |
| BmgT120I | GGNCC | 3 cut(s) | 155, 240, 241 |
| BmiI | GGNNCC | 2 cut(s) | 241, 242 |
| BmrFI | CCNGG | 4 cut(s) | 17, 99, 280, 323 |
| BpuMI | CCSGG | 4 cut(s) | 17, 99, 280, 323 |
| BsaHI | GRCGYC | 1 cut(s) | 147 |
| BsaJI | CCNNGG | 5 cut(s) | 16, 38, 219, 228, 321 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 132, 162 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 17, 104, 220, 250, 285, 350 |
| Bse118I | RCCGGY | 1 cut(s) | 82 |
| BseDI | CCNNGG | 5 cut(s) | 16, 38, 219, 228, 321 |
| BseGI | GGATG | 3 cut(s) | 7, 113, 241 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 17, 104, 220, 250, 285, 350 |
| BsePI | GCGCGC | 1 cut(s) | 379 |
| BseSI | GKGCMC | 1 cut(s) | 244 |
| Bsh1236I | CGCG | 2 cut(s) | 88, 381 |
| BshFI | GGCC | 2 cut(s) | 224, 242 |
| BsiHKAI | GWGCWC | 1 cut(s) | 265 |
| BsiHKCI | CYCGRG | 2 cut(s) | 243, 384 |
| BsiSI | CCGG | 5 cut(s) | 16, 83, 99, 279, 323 |
| BslFI | GGGAC | 2 cut(s) | 104, 304 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 17, 104, 220, 250, 285, 350 |
| BsmFI | GGGAC | 2 cut(s) | 104, 304 |
| BsnI | GGCC | 2 cut(s) | 224, 242 |
| BsoBI | CYCGRG | 2 cut(s) | 243, 384 |
| Bsp120I | GGGCCC | 1 cut(s) | 240 |
| Bsp1286I | GDGCHC | 2 cut(s) | 244, 265 |
| Bsp143I | GATC | 2 cut(s) | 32, 388 |
| BspACI | CCGC | 5 cut(s) | 88, 122, 192, 225, 348 |
| BspANI | GGCC | 2 cut(s) | 224, 242 |
| BspFNI | CGCG | 2 cut(s) | 88, 381 |
| BspLI | GGNNCC | 2 cut(s) | 241, 242 |
| BsrFI | RCCGGY | 1 cut(s) | 82 |
| BssAI | RCCGGY | 1 cut(s) | 82 |
| BssECI | CCNNGG | 5 cut(s) | 16, 38, 219, 228, 321 |
| BssHII | GCGCGC | 1 cut(s) | 379 |
| BssMI | GATC | 2 cut(s) | 32, 388 |
| BssNI | GRCGYC | 1 cut(s) | 147 |
| BssT1I | CCWWGG | 1 cut(s) | 219 |
| Bst4CI | ACNGT | 1 cut(s) | 337 |
| Bst6I | CTCTTC | 1 cut(s) | 284 |
| BstACI | GRCGYC | 1 cut(s) | 147 |
| BstC8I | GCNNGC | 1 cut(s) | 381 |
| BstDSI | CCRYGG | 1 cut(s) | 38 |
| BstF5I | GGATG | 3 cut(s) | 7, 113, 241 |
| BstFNI | CGCG | 2 cut(s) | 88, 381 |
| BstHHI | GCGC | 3 cut(s) | 88, 381, 383 |
| BstKTI | GATC | 2 cut(s) | 35, 391 |
| BstMBI | GATC | 2 cut(s) | 32, 388 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 12, 83, 224 |
| BstSCI | CCNGG | 4 cut(s) | 15, 97, 278, 321 |
| BstSLI | GKGCMC | 1 cut(s) | 244 |
| BstUI | CGCG | 2 cut(s) | 88, 381 |
| BstX2I | RGATCY | 1 cut(s) | 32 |
| BstYI | RGATCY | 1 cut(s) | 32 |
| BsuI | GTATCC | 1 cut(s) | 247 |
| BsuRI | GGCC | 2 cut(s) | 224, 242 |
| BtgI | CCRYGG | 1 cut(s) | 38 |
| BtsCI | GGATG | 3 cut(s) | 7, 113, 241 |
| Cac8I | GCNNGC | 1 cut(s) | 381 |
| CfoI | GCGC | 3 cut(s) | 88, 381, 383 |
| Cfr10I | RCCGGY | 1 cut(s) | 82 |
| Cfr13I | GGNCC | 3 cut(s) | 155, 240, 241 |
| Csp6I | GTAC | 1 cut(s) | 267 |
| CviJI | RGCY | 8 cut(s) | 6, 15, 61, 213, 218, 224, 242, 278 |
| CviKI_1 | RGCY | 8 cut(s) | 6, 15, 61, 213, 218, 224, 242, 278 |
| CviQI | GTAC | 1 cut(s) | 267 |
| DpnI | GATC | 2 cut(s) | 34, 390 |
| DpnII | GATC | 2 cut(s) | 32, 388 |
| EaeI | YGGCCR | 1 cut(s) | 222 |
| Eam1104I | CTCTTC | 1 cut(s) | 284 |
| EarI | CTCTTC | 1 cut(s) | 284 |
| Eco130I | CCWWGG | 1 cut(s) | 219 |
| Eco24I | GRGCYC | 1 cut(s) | 244 |
| Eco47I | GGWCC | 1 cut(s) | 155 |
| Eco88I | CYCGRG | 2 cut(s) | 243, 384 |
| EcoO109I | RGGNCCY | 1 cut(s) | 240 |
| EcoT14I | CCWWGG | 1 cut(s) | 219 |
| EcoT38I | GRGCYC | 1 cut(s) | 244 |
| ErhI | CCWWGG | 1 cut(s) | 219 |
| FaiI | YATR | 1 cut(s) | 104 |
| FaqI | GGGAC | 2 cut(s) | 104, 304 |
| FauI | CCCGC | 2 cut(s) | 81, 341 |
| Fnu4HI | GCNGC | 1 cut(s) | 225 |
| FokI | GGATG | 2 cut(s) | 120, 248 |
| FriOI | GRGCYC | 1 cut(s) | 244 |
| Fsp4HI | GCNGC | 1 cut(s) | 225 |
| FspBI | CTAG | 3 cut(s) | 363, 416, 439 |
| GlaI | GCGC | 3 cut(s) | 87, 380, 382 |
| GluI | GCNGC | 1 cut(s) | 225 |
| HaeIII | GGCC | 2 cut(s) | 224, 242 |
| HapII | CCGG | 5 cut(s) | 16, 83, 99, 279, 323 |
| HhaI | GCGC | 3 cut(s) | 88, 381, 383 |
| Hin1I | GRCGYC | 1 cut(s) | 147 |
| Hin6I | GCGC | 3 cut(s) | 86, 379, 381 |
| HinP1I | GCGC | 3 cut(s) | 86, 379, 381 |
| HincII | GTYRAC | 1 cut(s) | 187 |
| HindII | GTYRAC | 1 cut(s) | 187 |
| HinfI | GANTC | 3 cut(s) | 92, 317, 412 |
| HpaII | CCGG | 5 cut(s) | 16, 83, 99, 279, 323 |
| HphI | GGTGA | 2 cut(s) | 87, 337 |
| Hpy166II | GTNNAC | 2 cut(s) | 127, 187 |
| Hpy188I | TCNGA | 1 cut(s) | 12 |
| Hpy188III | TCNNGA | 3 cut(s) | 168, 386, 409 |
| Hpy8I | GTNNAC | 2 cut(s) | 127, 187 |
| Hpy99I | CGWCG | 1 cut(s) | 152 |
| HpyAV | CCTTC | 1 cut(s) | 315 |
| HpyCH4III | ACNGT | 1 cut(s) | 337 |
| HpyCH4IV | ACGT | 1 cut(s) | 147 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 12, 83, 224 |
| HpySE526I | ACGT | 1 cut(s) | 147 |
| Hsp92I | GRCGYC | 1 cut(s) | 147 |
| HspAI | GCGC | 3 cut(s) | 86, 379, 381 |
| Kzo9I | GATC | 2 cut(s) | 32, 388 |
| LpnPI | CCDG | 6 cut(s) | 29, 96, 112, 132, 292, 336 |
| MaeI | CTAG | 3 cut(s) | 363, 416, 439 |
| MaeII | ACGT | 1 cut(s) | 147 |
| MaeIII | GTNAC | 2 cut(s) | 205, 331 |
| MalI | GATC | 2 cut(s) | 34, 390 |
| MboI | GATC | 2 cut(s) | 32, 388 |
| MboII | GAAGA | 1 cut(s) | 301 |
| MflI | RGATCY | 1 cut(s) | 32 |
| MhlI | GDGCHC | 2 cut(s) | 244, 265 |
| MlyI | GAGTC | 2 cut(s) | 86, 326 |
| MnlI | CCTC | 8 cut(s) | 36, 48, 223, 279, 285, 310, 351, 417 |
| MspA1I | CMGCKG | 2 cut(s) | 122, 192 |
| MspI | CCGG | 5 cut(s) | 16, 83, 99, 279, 323 |
| MspR9I | CCNGG | 4 cut(s) | 17, 99, 280, 323 |
| MvnI | CGCG | 2 cut(s) | 88, 381 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 12, 83, 224 |
| NciI | CCSGG | 4 cut(s) | 17, 99, 280, 323 |
| NdeII | GATC | 2 cut(s) | 32, 388 |
| NlaIV | GGNNCC | 2 cut(s) | 241, 242 |
| NmeAIII | GCCGAG | 1 cut(s) | 253 |
| PaeR7I | CTCGAG | 1 cut(s) | 384 |
| PauI | GCGCGC | 1 cut(s) | 379 |
| PfeI | GAWTC | 1 cut(s) | 412 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 220 |
| PkrI | GCNGC | 1 cut(s) | 226 |
| PleI | GAGTC | 2 cut(s) | 86, 325 |
| PpsI | GAGTC | 2 cut(s) | 86, 325 |
| PspN4I | GGNNCC | 2 cut(s) | 241, 242 |
| PspOMI | GGGCCC | 1 cut(s) | 240 |
| PspPI | GGNCC | 3 cut(s) | 155, 240, 241 |
| PsuI | RGATCY | 1 cut(s) | 32 |
| PteI | GCGCGC | 1 cut(s) | 379 |
| RsaI | GTAC | 1 cut(s) | 268 |
| RsaNI | GTAC | 1 cut(s) | 267 |
| SatI | GCNGC | 1 cut(s) | 225 |
| Sau3AI | GATC | 2 cut(s) | 32, 388 |
| Sau96I | GGNCC | 3 cut(s) | 155, 240, 241 |
| SchI | GAGTC | 2 cut(s) | 86, 326 |
| ScrFI | CCNGG | 4 cut(s) | 17, 99, 280, 323 |
| SduI | GDGCHC | 2 cut(s) | 244, 265 |
| SetI | ASST | 7 cut(s) | 63, 150, 165, 234, 362, 375, 428 |
| Sfr274I | CTCGAG | 1 cut(s) | 384 |
| SinI | GGWCC | 1 cut(s) | 155 |
| SlaI | CTCGAG | 1 cut(s) | 384 |
| SmlI | CTYRAG | 1 cut(s) | 384 |
| SmoI | CTYRAG | 1 cut(s) | 384 |
| SsiI | CCGC | 5 cut(s) | 88, 122, 192, 225, 348 |
| SspMI | CTAG | 3 cut(s) | 363, 416, 439 |
| StyD4I | CCNGG | 4 cut(s) | 15, 97, 278, 321 |
| StyI | CCWWGG | 1 cut(s) | 219 |
| TaaI | ACNGT | 1 cut(s) | 337 |
| TaiI | ACGT | 1 cut(s) | 150 |
| TaqI | TCGA | 5 cut(s) | 23, 167, 201, 385, 391 |
| TauI | GCSGC | 1 cut(s) | 227 |
| TfiI | GAWTC | 1 cut(s) | 412 |
| TspGWI | ACGGA | 1 cut(s) | 27 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 220 |
| VpaK11BI | GGWCC | 1 cut(s) | 155 |
| XhoI | CTCGAG | 1 cut(s) | 384 |
| XspI | CTAG | 3 cut(s) | 363, 416, 439 |
| ZraI | GACGTC | 1 cut(s) | 148 |